• Expression of procollagen C-proteinase enhancer in cultured ra. (mysciencework.com)
  • Expression of PCP was assessed by RT-PCR and its activity in the culture media was determined using radioactive procollagen as the substrate. (mysciencework.com)
  • We have taken advantage of our previous finding that PDI interacts with procollagen chains early on in their assembly pathway to address the role of this protein in directly retaining unassembled chains within the ER. (nih.gov)
  • Identification and expression of a novel type I procollagen C-proteinase enhancer protein gene from the glaucoma candidate region on 3q21-q24. (wikigenes.org)
  • We also show how the crystal structure of the complex between the C-propeptide trimer of procollagen III and the active fragment of procollagen C-proteinase enhancer-1 leads to a detailed model for accelerating release of the C-propeptides from procollagen by bone morphogenetic protein-1 and related proteinases. (portlandpress.com)
  • The main proteinase involved in C-propeptide release is bone morphogenetic protein-1 (BMP-1) which is assisted by another ECM protein, procollagen C-proteinase enhancer-1 (PCPE-1) that binds strongly to the C-propeptides [ 4 ]. (portlandpress.com)
  • Defective folding and stable association with protein disulfide isomerase/prolyl hydroxylase of type I procollagen with a deletion in the pro alpha 2(I) chain that preserves the Gly-X-Y repeat pattern. (uniprot.org)
  • While the abnormal procollagen molecules were not bound by the ER-resident protein BiP, they stably associated with protein disulfide isomerase, the beta-subunit of prolyl-4-hydroxylase. (uniprot.org)
  • In enzymology, a procollagen glucosyltransferase (EC 2.4.1.66) is an enzyme that catalyzes the chemical reaction UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen ⇌ {\displaystyle \rightleftharpoons } UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen Thus, the two substrates of this enzyme are UDP-glucose and 5-(D-galactosyloxy)-L-lysine-procollagen, whereas its two products are UDP and 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen. (wikipedia.org)
  • The systematic name of this enzyme class is UDP-glucose:5-(D-galactosyloxy)-L-lysine-procollagen D-glucosyltransferase. (wikipedia.org)
  • Ordering To order Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 3 (PLOD3) Polyclonal Antibody , please use the Cat. (gentaur.com)
  • When cells were heat shocked, or when stable triple-helix formation was inhibited by treatment with alpha,alpha'-dipyridyl, coprecipitation of procollagen with HSP47 was increased. (rupress.org)
  • The procollagen ELISA takes advantage of a one-step format in which test samples and peroxidase-labeled anti-PIP are simultaneously added to the wells of the plate and incubated. (clontech.com)
  • We hypothesized that thrombin might also affect the deposition of connective tissue proteins at these sites by directly stimulating fibroblast procollagen production. (biochemj.org)
  • These results suggest that thrombin might influence the deposition of connective tissue proteins during normal wound healing and the development of tissue fibrosis by stimulating fibroblast procollagen production. (biochemj.org)
  • Chemical crosslinking studies were used to test this hypothesis both before and after the perturbation of procollagen processing. (rupress.org)
  • Those that form the regular banded fibrils seen by electron microscopy are synthesized as soluble procollagen molecules with large N- and C-propeptides flanking the triple-helical domain. (portlandpress.com)