Processing rna stability. Medical search. Frequent questions

30, but in urine, RNA degradation occurred more quickly. (cdc.gov)
A second major area of interest focuses on how proteins contribute to RNA stability and degradation. (mskcc.org)
CircRNAs are more resistant to degradation compared with mRNAs and exhibit high biological stability because of the complete circular covalently linked structure [ 7 , 8 ]. (hindawi.com)
Additional RNAs are involved in gene regulation and mRNA degradation. (dekooktips.com)
For example, the transcription, translation, and degradation of an mRNA is crucial to any gene expression event, and all three processes are controlled by a combination of elements including promoters, ribosome binding sites (RBSs), and cis -regulatory signals encoded in untranslated regions (UTRs). (universityofcalifornia.edu)

The remaining mitochondrial proteins, metabolic enzymes, DNA and RNA polymerases, ribosomal proteins, and mtDNA regulatory factors are all encoded by nuclear genes. (wikipedia.org)
DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex. (nih.gov)
They are messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). (dekooktips.com)
The other two forms of RNA, ribosomal RNA (rRNA) and transfer RNA (tRNA), are involved in the process of ordering the amino acids to make the protein. (dekooktips.com)
There are three types of RNA involved in the translation process: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). (dekooktips.com)
The three main types of RNA directly involved in protein synthesis are messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA). (dekooktips.com)
Messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA) are the three major types of RNA. (dekooktips.com)
Dyskerin is composed of 514 amino acids and has a role in ribosomal RNA processing and telomere maintenance. (medscape.com)

Messenger RNA (mRNA) is a single-stranded molecule naturally present in all of our cells. (medicalnewstoday.com)
RNA is a notoriously fragile molecule. (medicalnewstoday.com)
When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
This expression is strictly dependent on the synthesis of messenger RNAs transcripts from the DNA molecule and their subsequent translation into proteins by the ribosomes. (cea.fr)
Any process that decreases the stability of an RNA molecule, making it more vulnerable to degradative processes. (yeastrc.org)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
For early life to be built on RNA, though, this molecule must have had the ability to make copies of itself. (elifesciences.org)
This gene provides instructions for making a particular type of RNA, a molecule that is a chemical cousin of DNA. (medlineplus.gov)

RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
As they are synthesized, mRNAs undergo chemical modifications that ensure their stability, their delivery to ribosomes and eventually their translation into proteins. (cea.fr)
Our data reveals that rG4s can be found in messenger RNAs (mRNAs) and long intergenic noncoding RNAs (lincRNAs). (nih.gov)
Ribonucleic acids (RNAs) carry as messenger-RNAs (mRNAs) genetic information from DNA to cellular protein factories, where they are translated into proteins. (mpg.de)
Despite this, the mRNAs have concerns about their stability and immuno-stimulation, therefore, researchers and companies have concentrated their work to address this issue [2]. (researchgate.net)
Signal transduction pathways regulate gene expression in part by modulating the stability of specific mRNAs. (ed.ac.uk)
Nuclear mRNA transcripts acquire a protein coat composed of cap- and RNA-binding proteins that allow nuclear export and dictate the functional program of cytoplasmic mRNAs ( Moore, 2005 ). (rupress.org)
Some mRNAs are programmed for immediate translation, a process that remodels the protein coat and assembles a polysome. (rupress.org)

But they also have important regulatory functions: Small noncoding RNAs (miRNAs) influence mRNA stability and are able to switch off genes by stalling their translation into proteins. (mpg.de)
This is especially interesting, because over the past years there have been controversial discussions as to whether or not small noncoding RNAs occur in the nucleus. (mpg.de)

The expression levels of RNA of hsa_circ_0046060, mmu_circ_0002819, and miR-338-3p were determined by quantitative real-time polymerase chain reaction (RT-qPCR). (hindawi.com)
During evolution, this protein naturally lost its catalytic activity in Drosophila where Pcif1 is, like its human counterpart, expressed in the nucleus and associated with the C-terminal domain of RNA polymerase (RNA Pol II). (cea.fr)
This protein binds to the phosphorylated form of serine 5 of RNA polymerase II ( Figure ) and may directly modulate its activity or promote the recruitment of chromatin components. (cea.fr)
These results suggest a similar contribution of PCIF1 to the fine regulation of RNA polymerase II activity in mammals, in addition to its mRNA methylation activity, whose role in mRNA translation efficiency is actively studied but still controversial. (cea.fr)
87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
We report RNA-catalysed RNA synthesis on structured templates when using trinucleotide triphosphates (triplets) as substrates, catalysed by a general and accurate triplet polymerase ribozyme that emerged from in vitro evolution as a mutualistic RNA heterodimer. (elifesciences.org)
In addition, detection of viral RNA by reverse transcription--polymerase chain reaction (RT-PCR), usually in a throat swab or urine sample, and subsequent genotyping of strains is valuable for diagnosis and molecular epidemiology ( 2 ). (cdc.gov)

The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. (wiley-vch.de)
This m(6)A-dependent regulation of protein binding through a change in RNA structure, termed "m(6)A-switch", affects transcriptome-wide mRNA abundance and alternative splicing. (nih.gov)
Dr. Lima's laboratory uses structural, biochemical, and functional analyses of molecules involved in RNA processing and protein modification with a focus on understanding how these pathways contribute to cell growth and disease. (mskcc.org)
The RNA exosome is composed of many protein subunits that assemble into a unique arrangement capable of binding and degrading targeted RNA molecules. (mskcc.org)
Three main types of RNA are involved in protein synthesis. (dekooktips.com)
What are the three types of RNA and their role in protein synthesis? (dekooktips.com)
In 1961, French scientists François Jacob and Jacques Monod hypothesized the existence of an intermediary between DNA and its protein products, which they called messenger RNA. (dekooktips.com)
Two of these genes, DIS3 and FAM46C , appear to play important roles in the stability of RNA and hence its translation into protein. (broadinstitute.org)
Many different principles of thermoregulation have been identified in bacteria, which can be assigned to the class of protein-, DNA- or RNA-thermosensors. (springer.com)
Due to the inherent diversity in the underlying mechanisms, protein-based thermosensors affect different cellular processes such as transcription, translation, protein stability, signal transduction as well as proteolytic processes. (springer.com)
Studies carried out by scientists at IRIG, in collaboration with the University of Geneva, on the fruit fly model Drosophila melanogaster have revealed the role of Pcif1 in the control of gene expression, despite the fact that this protein has completely lost its RNA methyltransferase activity compared to its mammalian counterpart PCIF1. (cea.fr)
Researchers at IRIG, in collaboration with the University of Geneva, focused on a RNA mammalian methyltransferase, the PCIF1 protein (homologous to the Drosophila Pcif1 protein), which adds an extra methyl group to m 6 A (m 6 adenosine) to form m 6 Am when the first transcribed nucleotide is an adenosine. (cea.fr)
As far as humans are concerned, the argonaute protein Ago-2 is the key cellular binding partner of miRNAs: The Ago-miRNA complex binds to mRNA and impedes their translation into proteins - either by blocking the translation process or by initiating RNA decomposition. (mpg.de)
Abstract: RNA transcribed from the genome in the nucleus bears little resemblance to the RNA polymer it will ultimately become in the cytoplasm where it is translated into protein. (uci.edu)
These alterations regulate critical aspects of RNA function such as stability, transport, protein binding, and translation. (uci.edu)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
The zinc finger protein tristetraprolin (TTP) is expressed in response to LPS and regulates the stability of TNF-alpha mRNA. (ed.ac.uk)
The p38 pathway is required for the induction of TNF-alpha RNA-binding activity and for the expression of TTP protein and mRNA. (ed.ac.uk)
Our findings demonstrate a direct link between a specific signal transduction pathway and a specific RNA-binding protein, both of which are known to regulate TNF-alpha gene expression at a posttranscriptional level. (ed.ac.uk)
Genetic systems often behave unpredictably due to structural interactions between DNA, RNA and protein components as well as functional interactions with host factors and metabolites. (universityofcalifornia.edu)
Here I review recent work in yeast, flies, mice and primates that examines the influences of naturally occurring sequence variation, chromosomal order and speciation on genome-wide expression profiles of both RNA and protein. (biomedcentral.com)
Different classes of RNA granules share some protein components and may use similar mechanisms to regulate mRNA translation/decay. (rupress.org)
A neuron-specific in expression RNA-BINDING PROTEIN, involved in neurological disorders and cancer. (bvsalud.org)

The Ccr4-Not complex removes mRNA poly(A) tails to regulate eukaryotic mRNA stability and translation. (elifesciences.org)
Consequently, motif quality is a major determinant of mRNA stability for Puf3 targets in vivo and can be used for the prediction of mRNA targets. (elifesciences.org)
N6-Methyladenosine (m 6 A), a predominant internal modification of RNA in mammalian cells, has been recognized as having a vital role in mRNA stability, export, translation, splicing, and decay [ 4 ]. (hindawi.com)

Long non-coding RNAs (lncRNAs) govern fundamental biochemical and cellular processes. (biomedcentral.com)

Regulation of RNA stability and translation are two key steps of cellular homeostasis. (ens-lyon.fr)
To better apprehend how a RNA can act on gene expression regulation as part of a RiboNucleoProtein complex (RNP), we are also interested in specific RNP formations and processings. (ens-lyon.fr)
In this study, the temperature-induced regulation of this native RNA-thermometer for heterologous rhamnolipid production was evaluated and its potential application for process control is discussed. (springer.com)
A recently discovered dimension of post-transcriptional gene regulation involves co-regulatory crosstalk between RNA transcripts, which compete for common pools of microRNA (miRNA) molecules. (biomedcentral.com)
RNA structures are of crucial importance for biological function and gene regulation. (nih.gov)
Defects of these regulation processes may lead to cancer and neurodegenerative diseases. (mpg.de)
Cytoplasmic RNA granules in germ cells (polar and germinal granules), somatic cells (stress granules and processing bodies), and neurons (neuronal granules) have emerged as important players in the posttranscriptional regulation of gene expression. (rupress.org)

G-quadruplexes have been shown to be involved in a range of processes including telomere stability and RNA transcription, splicing, translation and transport. (nature.com)
What are the 3 types of RNA and what is the transcription process? (dekooktips.com)
DHX9 is an RNA/DNA helicase that is involved in multiple cellular processes, including transcription and maintaining genome stability. (gla.ac.uk)
In this study, we used real-time reverse transcription-PCR (RT-PCR) to measure levels of shed viral RNA in saliva, urine, and feces of subcutaneously inoculated bank voles until they were killed at day 133 postinfection (PI). (cdc.gov)

RNA is a single-stranded nucleic acid that is composed of three main elements: a nitrogenous base, a five-carbon sugar and a phosphate group. (dekooktips.com)
PIWI-interacting RNAs (piRNAs) are a large family of small, single-stranded, non-coding RNAs present throughout the animal kingdom. (biomedcentral.com)

In the majority of cases, Rf genes produce proteins that act directly on the CMS conferring mitochondrial transcripts by binding them specifically and promoting processing events. (frontiersin.org)
In the majority of cases, Rf genes produce proteins that bind specifically to the CMS conferring transcripts in the mitochondria and promote processing events leading to a strong reduction in the production of mitochondrial CMS-inducing proteins (reviewed in Chen and Liu, 2014 ). (frontiersin.org)

In the past, we have been working on two cellular factors, the translation initiation factor INT6/EIF3E and the RNA helicase UPF1, that act at the crossroads of RNA translation and decay. (ens-lyon.fr)
In addition to the RNA helicase UPF1, we develop an axis of research based on the G4 resolvase DHX36 (project 2) . (ens-lyon.fr)

CircRNAs are newly discovered noncoding RNAs formed through the alternative splicing of premessenger RNA (mRNA). (hindawi.com)
Additional links to NCBI resources are provided for human noncoding RNAs and repeated DNA elements and for proteins of interest from other species. (cshlpress.com)

In response to stimuli, the cell carefully regulates the abundance of certain messenger RNA by balancing the synthesis of new RNA against the destruction of old RNA once it has fulfilled its function. (mskcc.org)
An overview of how we approach process safety and a case study to demonstrate the application of our approach to a Barbier-type Grignard reaction in the synthesis of Kyprolis will be presented. (uci.edu)
The triplets cooperatively invaded and unraveled even highly stable RNA secondary structures, and support non-canonical primer-free and bidirectional modes of RNA synthesis and replication. (elifesciences.org)

The m(6)A-modified hairpin has a predisposed conformation that resembles the hairpin conformation in the RNA-HNRNPC complex more closely than the unmodified hairpin. (nih.gov)
The third class of thermosensing is based on the temperature-dependent conformation of specific RNA sequences, which are termed RNA-thermometer (RNAT). (springer.com)
Remarkably, we found that majority of our in vitro identified rG4s can change the in silico predicted RNA structure to uncover alternative RNA conformation. (nih.gov)

Since changes in RNA structure can affect diverse cellular processes, the influence of m(6)A on mRNA and lncRNA structure has the potential to be an important mechanism for m(6)A function in the cell. (nih.gov)
The m(6)A-induced structural changes in the MALAT1 hairpin can serve as a model for a large family of m(6)A-switches that mediate the influence of m(6)A on cellular processes. (nih.gov)
In half of the study's patients, the researchers found mutations in genes that control two fundamental cellular processes: how RNA is processed and proteins are folded. (broadinstitute.org)

A recently published study found that viral RNA was consistently detectable in blood of cynomolgus macaques until 3 weeks after euthanasia ( 6 ). (cdc.gov)
In addition, quantification (copies/mL) or cycle threshold (C t ) determination of EBOV RNA for estimating viral load has been shown to be a major prognosis marker in affected patients ( 1 , 7 , 8 ). (cdc.gov)
Moreover, C t value does not reflect viral viability but only presence of RNA in samples. (cdc.gov)
Levels of shed viral RNA peaked within 11-28, 14-21, and 11-28 days postinfection for saliva, urine, and feces, respectively. (cdc.gov)
In contrast, blood of 5 of 6 animals contained viral RNA at day 133 postinfection, suggesting that bank voles secrete virus only during a limited time of the infection. (cdc.gov)
To evaluate possible transmission routes for PUUV, we investigated infectivity of different excretions and used a subset of viral RNA-positive saliva, urine, and feces samples to intranasally inoculate virus-negative bank voles. (cdc.gov)
Accurate laboratory results for detection of IgM and viral RNA are dependent on proper collection, processing, shipment, and storage of clinical samples and use of accurate tests performed by a proficient laboratory. (cdc.gov)
DBS has been used for various epidemiologic studies for the detection of measles- and rubella-specific IgG and IgM antibodies and viral RNA ( 3--5 ). (cdc.gov)

RNA, or ribonucleic acid, has numerous functions in the cell including the ability to convey genetic information in a form called messenger RNA, which is read by ribosomes to make proteins. (mskcc.org)

Predicted to be involved in rRNA processing. (nih.gov)
RNA processing in chloroplasts includes mRNA 5′- and 3′-end processing, intron splicing, and intercistronic cleavages of polycistronic messages, as well as typical tRNA and rRNA processing. (dekooktips.com)
There are three types of RNA: mRNA, tRNA, and rRNA. (dekooktips.com)
Furthermore, knockdown of Nop2 results in global reduction of all RNA species, including rRNA, small nuclear RNA, small nucleolar RNA, and mRNA. (umass.edu)

Work in the Lima lab has focused on how an RNA-degrading complex called the RNA exosome targets these RNA molecules for destruction. (mskcc.org)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)

The nucleus also contains a number of distinct subnuclear foci known as nuclear bodies, which are dynamically controlled structures that help numerous nuclear processes run more efficiently. (wikipedia.org)
G-rich sequences have a propensity for forming highly stable quadruplex structures in both RNA and DNA termed G-quadruplexes. (nature.com)
RNA is then translated into proteins by structures called ribosomes. (dekooktips.com)
We apply this method to human RNA and report the first global map of rG4 structures for any organism. (nih.gov)
We also find that rG4 formation is associated with its stability, Cytosine (C)-content, and the stability of alternative RNA structures. (nih.gov)
However, regulatory elements can unpredictably interact with each other through the formation of RNA structures and the recruitment of factors that affect global transcript accessibility and stability. (universityofcalifornia.edu)
However, RNA catalysis is dependent upon RNA folding, and this yields structures that can block replication of such RNAs. (elifesciences.org)
Yet, some RNA strands can also fold to form ribozymes, 3D structures that could have guided life's chemical processes the way proteins do now. (elifesciences.org)
For example, these triplets work together to bind tightly to RNA strands and unravel structures that block RNA copying. (elifesciences.org)

N(6)-Methyladenosine (m(6)A) is a reversible and abundant internal modification of messenger RNA (mRNA) and long noncoding RNA (lncRNA) with roles in RNA processing, transport, and stability. (nih.gov)
As a result, circRNAs exert important roles in numerous physiological and pathological processes [ 9 , 10 ]. (hindawi.com)
What are the three main types of RNA describe their roles quizlet? (dekooktips.com)
rG4-seq is a broadly applicable method that enables the RNA G4 structurome and its biological roles to be interrogated and can be readily applied in other organisms on a transcriptome-wide scale. (nih.gov)
Presenting the genes of the human genome in their biological context, Guide to the Human Genome is an extensive online resource (also available in print) that provides easy access to information about human genes and their roles in specific processes. (cshlpress.com)

The majority of proteins in a cell are the product of messenger RNA transcribed from nuclear genes, including most of the proteins of the organelles, which are produced in the cytoplasm like all nuclear gene products and then transported to the organelle. (wikipedia.org)
In the recent past, the presence and function of a ROSE-like RNA-thermometer located in the 5′UTR of the rhamnosyltransferase genes rhlAB has been reported in wild type P. aeruginosa . (springer.com)
Futhermore, the rG4s that have analogues in many ortholog genes across different species show a preferential association with RNA processing and stability. (nih.gov)
With numerous illustrations and tables, each of the nearly 300 sections of the Guide describes genes involved in a specific pathway, process, or structure from the molecular and cellular levels to developmental and physiological processes. (cshlpress.com)
The Guide will be useful to researchers looking to connect sequence data with functional information, and can be used in parallel with traditional texts in undergraduate and graduate courses to provide a genomics dimension and experience of identifying genes underpinning processes of interest. (cshlpress.com)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)

Messenger RNA (mRNA) Carries information specifying amino acid sequences of proteins from DNA to ribosomes. (dekooktips.com)
We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)

lncRNA HOX transcript antisense RNA (HOTAIR) represses gene expression through recruitment of chromatin modifiers. (biomedcentral.com)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)

We review the relationship between different classes of these granules and discuss how spatial organization regulates messenger RNA translation/decay. (rupress.org)

the CRISPR-associated nuclease Cas9 (SpCas9), a specificity-determining CRISPR RNA (crRNA), and an auxiliary trans-activating crRNA (tracrRNA) 11 . (cdc.gov)

RNA is inherently less stable than DNA due to its chemical structure. (idtdna.com)

and nucleotides at the 3′ end regulate the stability of the mRNA (Fig. 1.1). (dekooktips.com)

In nature, the control of the activity of an RNA transcript is crucial to its function. (universityofcalifornia.edu)

We evaluated RNA stability of Ebola virus in EDTA blood and urine samples collected from infected patients and stored in West Africa's environmental conditions. (cdc.gov)
By using C t determination, we attempted to assess the stability of EBOV RNA in EDTA plasma (widely used in the field) and in urine from infected patients in the environmental conditions (i.e., air temperature and humidity) of West Africa. (cdc.gov)
We measured EBOV RNA stability in blood and urine samples from 7 case-patients with laboratory-confirmed Ebola. (cdc.gov)
Stability of Zika Virus in Urine: Specimen Processing Considerations and Implications for the Detection of RNA Targets in Urine. (cdc.gov)

Messenger RNA (mRNA) carries the instructions from the nucleus to the cytoplasm. (dekooktips.com)
mRNA is produced in the nucleus, as are all RNAs. (dekooktips.com)
The fly Pcif1 expressed in the nucleus and binds the phosphorylated C-terminal domain (CTD) of RNA Pol II (at the level of phosph orylated s erine 5). (cea.fr)

This biological process - and the enzymes that control it - had been studied for 30 years prior, but remained a mystery to researchers in the field until their work was described in two related studies published in Nature and the Journal of the American Chemical Society . (mskcc.org)

We identified Nop2 as an essential gene for development to the blastocyst stage while performing an RNA interference (RNAi)-based screen in mouse preimplantation embryos. (umass.edu)

In RIT's biotechnology and molecular biology degree you'll improve human health by harnessing technology advancements and biomolecular processes to research and develop technologies in genetics, agriculture, pharmaceuticals and vaccine development, environment and energy, forensic science, genetic counseling, and more. (rit.edu)
Like DNA, strands of RNA contain genetic data. (elifesciences.org)

While there are a lot of studies concerning miRNA processing, the target mRNA recognition and binding by the Ago-miRNA-complex is only poorly understood", says Gunter Meister, the head of the research group "RNA biology" at the Max Planck Institute. (mpg.de)
RNA-catalyzed RNA replication is widely believed to have supported a primordial biology. (elifesciences.org)

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, and N6-methyladenosine (m 6 A) is a predominant internal modification of RNA in various cancers. (hindawi.com)

Well-known processes such as capping, splicing and polyadenylation, as well as the recently discovered and ever-expanding list of diverse chemical modifications and editing, significantly alter the properties and fates of a given RNA during the course of its lifetime. (uci.edu)

Triplet substrates thus resolve a central incongruity of RNA replication, and here allow the ribozyme to synthesise its own catalytic subunit '+' and '-' strands in segments and assemble them into a new active ribozyme. (elifesciences.org)

A biological process that is crucial for gene expression in most animal life, including humans. (genomicglossaries.com)

Lawyer Tom Renz has exposed that the COVID-19 vaccines, widely advertised as mRNA (messenger RNA) vaccines, are in fact lab-created hybrids known as modRNA. (republicbroadcasting.org)
They claimed the COVID-19 vaccines were mRNA & that meant MESSENGER RNA (which occurs in life everywhere). (republicbroadcasting.org)
modRNA (modified messenger RNA) is a synthesized form of mRNA that has been altered at specific sites. (republicbroadcasting.org)
Pfizer admitted that during its clinical studies, participants aged 16 years and older received 30 mcg of nucleoside-modified messenger RNA (modRNA) . (republicbroadcasting.org)
Nucleoside-modified messenger RNA (modRNA) is a modified form of mRNA that encodes the spike (S) glycoprotein of the SARS-CoV-2 virus, the virus that causes COVID-19. (republicbroadcasting.org)
Messenger RNA (mRNA) vaccines represent a relatively new vaccine class showing great promise for the future. (researchgate.net)

For a long time these posttranscriptional processes were considered as intermediary minor steps of gene expression. (ens-lyon.fr)
In living organisms, gene expression is a complex process that results in the production of proteins from the genome in a time- and space-regulated manner. (cea.fr)
Our comprehensive analysis of the whole genome of F. equiseti provides basic data for studies of gene expression, regulatory and functional mechanisms, evolutionary processes, as well as disease prevention and control. (researchsquare.com)
In this section, we comprehensively analyzed the expression levels of fourteen m 6 A RNA methylation regulators and clinical factors in patients with HCC from the ICGC (International Cancer Genome Consortium, https://icgc.org/ ), Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/ ), and TCGA (The Cancer Genome Atlas, http://cancergenome.nih.gov/ ) databases. (hindawi.com)

Nuclear cells such as platelets do not possess nuclear DNA and therefore must have alternative sources for the RNA that they need to generate proteins. (wikipedia.org)
The introns containing these large expansions are transcribed and indeed in patients the GGGGCC-repeat expansion is detectable by in situ hybridization in nuclear RNA foci 5 . (nature.com)
To further characterize this first example of an m(6)A-switch in a cellular RNA, we used nuclear magnetic resonance and Förster resonance energy transfer to demonstrate the effect of m(6)A on a 32-nucleotide RNA hairpin derived from the m(6)A-switch in MALAT1. (nih.gov)

The pleiotropic effects of both proteins are perfect examples of the importance of RNA decay and translation in maintaining correct cell functioning. (ens-lyon.fr)
What are the three types of RNA involved in translation? (dekooktips.com)

In the autosomal dominant form, mutations in the RNA component of telomerase ( TERC ) or telomerase reverse transcriptase ( TERT ) are responsible for disease phenotype. (medscape.com)

With maximum values of 0.23 g/(g h) at 37 °C, this constitutes a more than 60% increase compared to the production rate of 0.14 g/(g h) at the growth optimum of 30 °C. Interestingly however, control experiments unveiled that besides the regulatory effect of the RNA-thermometer, multiple metabolic effects may contribute equally to the observed increase in production rate. (springer.com)

High-throughput RNA sequencing and bioinformatics analysis have led to the identification of several novel circRNAs that exhibit biological characteristics and regulatory functions [ 6 , 9 ]. (hindawi.com)
Whereas it takes many years to develop a vaccine and gain approval, largely because of the time it takes to conduct clinical trials, fully developing the large-scale manufacturing process intended for commercial production has always been a multiyear effort because of the science and engineering required to develop a robust process and the regulatory requirements for approval. (nature.com)
Methods and/or tools to facilitate the combination of various regulatory elements originating from various different sources to predictably control the activity of any desired RNA would be beneficial for numerous biotechnology applications. (universityofcalifornia.edu)
The invention allows for the combination of multiple regulatory elements in a fashion that predictably affects RNA activity. (universityofcalifornia.edu)

GUILDFORD, United Kingdom, Sept. 18, 2023 (GLOBE NEWSWIRE) -- SiSaf Ltd, an RNA therapeutics company, is pleased to announce the publication in the peer-reviewed journal Molecular Therapy Nucleic Acids (1) of positive in vivo data demonstrating the safety and efficacy of its siRNA therapy for Autosomal Dominant Osteopetrosis 2 (ADO2). (wane.com)
SiSaf is an RNA therapeutics company with a proprietary delivery platform. (wane.com)
The development of lipid nanoparticle (LNP) based therapeutics for delivery of RNA has triggered the advance of new strategies for formulation, such as high throughput microfluidics for precise mixing of components into well-defined particles. (lu.se)

However recent works clearly demonstrate that RNA stability per-se is critical for cell homeostasis. (ens-lyon.fr)
Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
Taken together, our results demonstrate that Nop2 is an essential gene for blastocyst formation, and is required for RNA processing and/or stability in vivo during preimplantation embryo development in the mouse. (umass.edu)

In the field in West Africa and notably in Guinea, most Ebola treatment units are located near (often in the same compound) a laboratory that can process collected samples within hours. (cdc.gov)
Few laboratory studies have investigated Ebola virus RNA stability over time in collected samples. (cdc.gov)

All life on Earth today uses a triplet RNA code to make proteins from DNA, and these experiments showed how RNA triplets might have helped RNA sustain early life forms. (elifesciences.org)

Since 2001, LabNet reference laboratories in Australia, Cote d'Ivoire, Netherlands, Turkey, Uganda, the UK, and the United States have been working to 1) determine IgM and RNA stability in DBS and OF samples and 2) optimize the methods for IgM antibody assay and protocols for RNA detection in DBS and OF samples ( 8--10 ). (cdc.gov)

Ribonucleotides, units of RNA, can become embedded in genomic DNA during processes such as DNA replication and repair, affecting the stability of the genome by contributing to DNA fragility and mutability. (labmanager.com)
The Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of singleguide RNAs (sgRNAs) to enable genome editing 1 - 10 . (cdc.gov)

Biochemical techniques were used to engineer a ribozyme that copies RNA strands by adding letters not one-by-one, but three-by-three. (elifesciences.org)

Chemical modifications during the manufacturing process can significantly improve the stability of mRNA vaccines. (medicalnewstoday.com)

Most of the m 6 A RNA methylation regulators were confirmed to be differentially expressed among groups stratified by clinical characteristics and tissues. (hindawi.com)
In summary, we demonstrated the vital role of m 6 A RNA methylation regulators in the initial presentation and progression of HCC and constructed a nomogram which would predict the clinical outcome and provide a basis for individualized therapy. (hindawi.com)

In this JoVE video, we will be sharing with you information about the collection and processing of lymph nodes from large animals for use in downstream RNA experiments. (jove.com)
For example, these experiments might include quantitative PCR on reverse transcribed products, or RNA sequencing studies of transcriptomes. (jove.com)

After acute injury in muscle of both mouse and the fruit fly Drosophila melanogaster (used as alternative in vivo model) this study found that RACK1 accumulated in regenerating fibers while it declined with the progression of repair process. (sdbonline.org)

More recently, another group of researchers reported that they had developed a process for optimizing low- abundance RNA, by combining aRNA amplification with template- switching. (genomicglossaries.com)

IGF2BP3 increased the stability of the EIF5B mRNA in an m6A-dependent manner. (techscience.com)
We find that SpCas9 tolerates mismatches between guide RNA and target DNA at different positions in a sequence-dependent manner, sensitive to the number, position and distribution of mismatches. (cdc.gov)

Anatomy5

Organisms5

Diseases1

Chemicals and Drugs61

Analytical, Diagnostic and Therapeutic Techniques and Equipment18

Phenomena and Processes50

Information Science3

Health Care2

Degradation5

Ribosomal8

Molecule9

MRNAs8

Small nonc2

Polymerase8

Protein23

MRNA stability3

Long non-codi1

Regulation7

Transcription4

Single-stranded2

Transcripts2

Helicase2

Noncoding RNAs2

Synthesis3

Conformation3

Cellular processes3

Viral8

Ribonucleic acid1

RRNA4

Molecules3

Structures9

Roles5

Genes6

Sequences2

Antisense2

Regulates1

Specificity1

Stable1

Regulate1

Transcript1

Urine4

Nucleus3

Enzymes1

Interference1

Genetic2

Biology2

Modification1

Polyadenylation1

Assemble1

Biological process1

Messenger6

Expression4

Nuclear3

Translation2

Mutations1

Metabolic1

Regulatory4

Therapeutics3

Demonstrate3

Ebola2

Make proteins1

Detection1

Genomic2

Biochemical1

Vaccines1

Regulators2

Experiments2

Vivo1

Abundance1

Dependent2