Processes rna 3. Medical search. Frequent questions

Both RRMs follow this pattern: β1-α1-β2-β3-α2-β4-β5, which allows them to bind to both RNA and DNA onto U G/T G-repeats of 3'UTR (Untranslated Terminal Regions) end of mRNA/DNA. (wikipedia.org)
These sequences mainly ensure mRNA processing, RNA export and RNA stabilizing. (wikipedia.org)
The mRNA processing reactions thus serve as a critical check point in viral RNA synthesis which may control the synthesis of incorrectly modified RNAs. (harvard.edu)
The minimal RNA length required for mRNA cap addition was also sufficient for methylation since the 31-nucleotide long transcripts were methylated at both ribose-2'-O and guanine-N-7 positions. (harvard.edu)
The 3' regulatory regions have a great diversity of cis -regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. (frontiersin.org)
Polyadenylation is essential for the stability of the transcript, preventing the mRNA from being the target of posttranscriptional gene silencing (PTGS) via RNA-dependent RNA polymerase 6 (RDR6) in plants ( Luo and Chen, 2007 ). (frontiersin.org)
CircRNAs are newly discovered noncoding RNAs formed through the alternative splicing of premessenger RNA (mRNA). (hindawi.com)
The mRNA acquires a 5' prime cap and 3' prime poly-A tail for protection and translation promotion. (khanacademy.org)
Chen J, Wang S, Jia S, Ding G, Jiang G, Cao L. Integrated Analysis of Long Non-Coding RNA and mRNA Expression Profile in Pancreatic Cancer Derived Exosomes Treated Dendritic Cells by Microarray Analysis. (jcancer.org)
For example, exosomes and nanovesicles secreted by living cells serve as intercellular couriers of mRNA, microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and proteins and have thus garnered considerable interest [ 3 ]. (jcancer.org)
Lawyer Tom Renz has exposed that the COVID-19 vaccines, widely advertised as mRNA (messenger RNA) vaccines, are in fact lab-created hybrids known as modRNA. (republicbroadcasting.org)
They claimed the COVID-19 vaccines were mRNA & that meant MESSENGER RNA (which occurs in life everywhere). (republicbroadcasting.org)
modRNA (modified messenger RNA) is a synthesized form of mRNA that has been altered at specific sites. (republicbroadcasting.org)
Nucleoside-modified messenger RNA (modRNA) is a modified form of mRNA that encodes the spike (S) glycoprotein of the SARS-CoV-2 virus, the virus that causes COVID-19. (republicbroadcasting.org)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
They found that one round of amplification produced approximately 10 3 fold of the estimated amount of starting mRNA, and two rounds produced an approximately 10 5 fold increase. (genomicglossaries.com)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)
A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
What parts will be included in the processed mRNA? (chiyuanye.com)
A cancer vaccine based on the messenger RNA (mRNA) technology, provided alongside the checkpoint inhibitor pembrolizumab (Keytruda), has shown encouraging results in an open label phase 2b clinical trial. (medscape.com)
Moderna is still developing a phase 3 clinical trial for mRNA-4157/V940, which the company hopes to launch sometime in 2023, Holen said during a press conference. (medscape.com)

Interestingly, one member of this module, Polr2a, polymerase (RNA) II (DNA directed) polypeptide A, is downregulated by the combination of prenatal ethanol and postnatal stress in an RNA-Seq experiment and qPCR validation (q = 2e-12, p = 0.004, respectively). (bepress.com)
The RNA synthesis machinery of vesicular stomatitis virus (VSV) comprises the genomic RNA encapsidated by the viral nucleocapsid protein (N) and associated with the RNA dependent RNA polymerase, the viral components of which are a large protein (L) and an accessory phosphoprotein (P). The 241 kDa L protein contains all the enzymatic activities necessary for synthesis of the viral mRNAs, including capping, cap methylation and polyadenylation. (harvard.edu)
This work provides insights into the spatial relationship between the active sites for the RNA dependent RNA polymerase and polyribonucleotidyltransferase responsible for capping of the viral RNA. (harvard.edu)
A new yeast poly(A) polymerase complex involved in RNA quality control. (nature.com)
The expression levels of RNA of hsa_circ_0046060, mmu_circ_0002819, and miR-338-3p were determined by quantitative real-time polymerase chain reaction (RT-qPCR). (hindawi.com)
Tracy says that the poly-A tail helps to promote termination of transcription by the RNA polymerase. (khanacademy.org)
If a promoter were to be after the operator, then a repressor (which binds to the operator) would have no effect on transcription because it would not block the RNA polymerase. (khanacademy.org)
87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
The MIT physicist has demonstrated the importance of clusters of RNA polymerase and other transcription mediators in regulating RNA production. (the-scientist.com)
Most importantly, we demonstrate that uridylation mediates DIS3L2 degradation of short RNA polymerase II-derived RNAs. (nih.gov)
A BRD4-mediated elongation control point primes transcribing RNA polymerase II for 3′-processing and termination. (mpg.de)
One of the main reasons that the virus is challenging, is due to its' RNA polymerase. (cdc.gov)

The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. (nih.gov)
In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)
Engineering RNA-Binding Proteins by Modular Assembly of RanBP2-Type Zinc Fingers. (rochester.edu)
DNA transcribes into RNA, with exons coding for proteins and introns removed by spliceosomes. (khanacademy.org)
These processes involve communication between the mitochondria and the rest of the cell via signaling pathways on the mitochondrial surface that mediate interactions with cytoplasmic proteins. (stonybrook.edu)
Our experimentation uncovered a new role for mitochondria in a specialized form of RNA-processing that appears to take place at the interface between the mitochondrial surface and adjacent granules of RNA and RNA-associated proteins," says Dr. Frohman, summarizing the research results. (stonybrook.edu)
Or you may see its SNR and P. And this is the combination of the small nuclear RNA and the proteins that make up the splices own. (pearson.com)
ELAV/Hu proteins comprise a family of highly conserved neuronal RNA binding proteins important for the development of the nervous system and for neuronal functions. (findaphd.com)
His fields of research comprise RNA-mediated gene silencing processes with a focus on epigenetic phenomena, including studies on RNA-directed DNA methylation, the characterization of virus silencing suppressor proteins, the development of plant bioreactor platforms and viroid research. (degruyter.com)
Viroids are non-encapsidated, single-stranded (ss), 250-400 nucleotide (nt)-long circular RNA molecules that do not encode proteins (1, 2). (degruyter.com)
If initial targeted genetic testing (either single gene or a panel of LGMDs) is negative, a muslce biopsy showld be obtained to look at the immunohistochemical staining patterns using antibodies directed at known disease associated proteins (e.g. dystrophin, sarcoglycans, merosin, α-dystroglycan, dysferlin, cveloin-3, etc) and to look for distinguishing features (e.g. rimmed vacuoles, myofibrillar myopathy). (medscape.com)

We use RNA-seq followed by clustering of expression profiles through weighted gene co-expression network analysis (WGCNA) to analyze transcriptomic changes that result from the treatments. (bepress.com)

The expression profiles of long noncoding RNAs (lncRNAs) and mRNAs of PEX-treated dendritic cells are unknown. (jcancer.org)
GO and pathway analysis revealed differentially expressed mRNAs involved in many critical biological processes and molecular functions. (jcancer.org)
In mammals, uridylated pre-let-7 miRNAs and mRNAs are targeted by the 3' to 5' exoribonuclease DIS3L2. (nih.gov)
Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. (bvsalud.org)
Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. (bvsalud.org)

2022 March 7 (3)Epub 01/22/2022. (rochester.edu)

Since then, he has been working as a postodoc in Dr. Wassenegger's lab (RLP AgroScience GmbH, AlPlanta) and his main field of interest is RNA-interference (RNAi) and RNA-directed DNA methylation (RdDM) in plants. (degruyter.com)
At least in plants, DNA regions that become de novo methylated can be defined by homologous RNA molecules in a process termed RNA-directed DNA methylation (RdDM). (degruyter.com)
The recruitment of this machinery results in the production of viroid-derived small RNAs (vd-sRNAs) that mediate RNA degradation and DNA methylation of cognate sequences. (degruyter.com)

Wang, X., Jia, H., Jankowsky, E. & Anderson, J.T. Degradation of hypomodified tRNA(iMet) in vivo involves RNA-dependent ATPase activity of the DExH helicase Mtr4p. (nature.com)
RNA degradation by the exosome is promoted by a nuclear polyadenylation complex. (nature.com)
Callahan, K.P. & Butler, J.S. TRAMP complex enhances RNA degradation by the nuclear exosome component Rrp6. (nature.com)
And that cap protects the RNA for degradation. (pearson.com)
Uridylation of various cellular RNA species at the 3' end has been generally linked to RNA degradation. (nih.gov)

Those RNA processing reactions are intimately coordinated with nucleotide polymerization such that failure to cap results in termination of transcription and failure to methylate can result in hyper polyadenylation. (harvard.edu)
Dr. Frohman explained that the significance of the finding is that piRNAs are known for suppressing cellular transcription (copying of RNA) and thus mobilization of genetic elements known as transposons, which make up almost half of the human genome. (stonybrook.edu)
So after transcription RNA has to undergo through um a few various processing steps before translation can occur because the M. RNA when it's trans transcribed is not at all ready to be translated. (pearson.com)
Once the TATA bos is exposed, TFIID come to bind the promotor and then recruit RNA Pol to come and start transcription. (chiyuanye.com)

The availability of multiple PAS in the same 3' regulatory region enables the inclusion or exclusion of 3' untranslated region (3'UTR) sequences, resulting in transcripts that may differ in particularities involving post-transcriptional processes such as stability, transport and translation ( Mayr, 2016 ), and even protein localization ( Berkovits and Mayr, 2015 ). (frontiersin.org)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
And pretty much when you have an M. RNA transcript it looks kind of like this where you have the coding sequences the exxons intertwined with the N. Tron and that's what the entire transcript looks like. (pearson.com)
RNA sequences that serve as templates for protein synthesis. (bvsalud.org)

DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex. (nih.gov)
Sloan, K.E., Bohnsack, M.T., Schneider, C. & Watkins, N.J. The roles of SSU processome components and surveillance factors in the initial processing of human ribosomal RNA. (nature.com)

The conserved Saccharomyces cerevisiae Ski2-like RNA helicase Mtr4p plays essential roles in eukaryotic nuclear RNA processing. (nature.com)
Our analysis of this data reveals an absolute protein-to-RNA ratio of 10 2 -10 4 for bacterial populations and 10 3 -10 5 for an archaeon, which is more comparable to Eukaryotic representatives' humans and yeast. (nature.com)

While the field of microbiology has adapted to the study of complex microbiomes via modern meta-omics techniques, we have not updated our basic knowledge regarding the quantitative levels of DNA, RNA and protein molecules within a microbial cell, which ultimately control cellular function. (nature.com)
Viroids are pathogenic circular, non-coding, single-stranded RNA molecules. (degruyter.com)
Upon inoculation, mature Pospiviroidae RNA molecules enter the plant cell nucleus. (degruyter.com)

The RNA recognition motif ranges between residues 105 and 181, much like many hnRNPs, TDP-43's RRMs encompass highly conserved motifs of primary importance for fulfilling their function. (wikipedia.org)

3: 100210, 2023 Dec 15. (bvsalud.org)

Pfizer admitted that during its clinical studies, participants aged 16 years and older received 30 mcg of nucleoside-modified messenger RNA (modRNA) . (republicbroadcasting.org)

A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. (nih.gov)
All of this raises the possibility that NTD and the RNA Recognition Motifs (later on defined) could cooperatively interact with nucleic acids to accomplish TDP-43's physiological functions. (wikipedia.org)

It corrects an error in what scientists call RNA splicing that prevents people with FD from manufacturing sufficient functional IKAP protein. (news-medical.net)
In their work on SMA, Krainer's team had shown that an ASO designed to bind to an RNA near a defective splice site could correct splicing and induce production of functional, full-length protein. (news-medical.net)
Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis. (nih.gov)
The protein sequence also has a nuclear localization signal (NLS, residues 82-98), a former nuclear export signal (NES residues 239-250) and 3 putative caspase-3 cleavage sites (residues 13, 89, 219). (wikipedia.org)
Here we report the temporal measurements of absolute RNA and protein levels per gene within a mixed bacterial-archaeal consortium. (nature.com)
RNA/protein levels) to highlight phenotypical complementarity. (nature.com)
For example, when Escherichia coli is grown axenically in steady state, we can expect that each RNA molecule corresponds to 10 2 -10 4 of the matching protein (absolute protein-to-RNA ratio, hereafter referred protein-to-RNA ratio) and the variation in the level of cellular RNA explains ~29% of the variation in the amount of detectable protein 2 . (nature.com)
In this context, the exploration of temporal interplay between populations with different lifestyles (comprising metabolism, motility, sporulation, etc.) becomes of primary importance to interpret the changes in fundamental quantities in a microbial community, such as the protein-to-RNA ratio that ultimately impacts the overarching community phenotype(s). (nature.com)
In order to perform studies of such design and test if previously defined quantitative data about the functioning of microorganisms (i.e., protein-to-RNA ratio) is applicable to real world consortia, we must first sample microbial communities across transition events and employ quantification techniques that are absolute. (nature.com)
Indeed, the protein-to-RNA ratio per sample of the bacterial populations matched previous calculations for the existing example from axenically cultured E. coli 2 . (nature.com)
The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. (wiley-vch.de)
1 It turns out that the bacterial genome has a few high-level instructions that control a few middle-level processes, that in turn control a massive number of protein-coding genes. (creation.com)
Presumably, this process involves the interaction between the host's bromodomain-containing viroid RNA-binding protein 1 (VIRP1) and the viroid's TR domain (6). (degruyter.com)

We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
To identify genes, signaling pathways and networks that are controlled by SRC-3 and potentially important for hormone-dependent decidualization, we performed RNA-sequencing on HESCs in which SRC-3 levels were significantly reduced at the time of administering the deciduogenic stimulus. (frontiersin.org)
Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)

3' Untranslated regions (3' UTRs) length is regulated in relation to cellular state. (nih.gov)
We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)
We show here that, like SRC-2, SRC-3 is expressed in the epithelial and stromal cellular compartments of the human endometrium during the proliferative and secretory phase of the menstrual cycle as well as in cultured HESCs. (frontiersin.org)
These molecular findings are supported at the cellular level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell when endogenous SRC-3 levels are markedly reduced. (frontiersin.org)
These predictive bioanalytic results were confirmed by the demonstration that SRC-3 is required for the expansion, migratory and invasive activities of the HESC population, cellular properties that are required in vivo in the formation or functioning of the decidua. (frontiersin.org)
5-FU inhibits tumor cell growth through at least 3 different mechanisms that ultimately disrupt DNA synthesis or cellular viability. (medscape.com)
Britt Glaunsinger discusses how viruses steal from their hosts to take over cellular processes and promote their own survival. (the-scientist.com)

Among persons aged 6 years and older who participated in NHANES during the 2013-2014 and 2015-2016 cycles, antibody confirmation testing was conducted using a 3rd generation commercial line immunoassay test for the subset of participants whose serum was reactive using a hepatitis C virus (HCV) antibody screening procedure, subsequently found to be negative for HCV RNA. (cdc.gov)
Samples with positive antibody confirmation results were then tested with a nucleic acid amplification test for hepatitis C virus RNA. (cdc.gov)

QIAseq UPX 3' Targeted RNA Panels use unique molecular index (UMIs) and ultraplex (UPX) technology to enable accurate gene expression using 3' RNA-seq from single cells (1-1000), cell pellets and previously isolated total RNA (1 pg to 10 ng). (qiagen.com)
Although a small number of 3' regulatory regions have been identified and validated so far, many studies have shown that plant 3' regulatory regions have a higher potential to regulate gene expression in plants compared to widely used 3' regulatory regions, such as NOS and OCS from Agrobacterium tumefaciens and 35S from cauliflower mosaic virus. (frontiersin.org)
In this review, we discuss the role of 3' regulatory regions in gene expression, and the superior potential that plant 3' regulatory regions have compared to NOS, OCS and 35S 3' regulatory regions. (frontiersin.org)
A biological process that is crucial for gene expression in most animal life, including humans. (genomicglossaries.com)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
Gene expression studies provide useful information about normal processes in the skin and responses of the skin to exogenous chemicals. (cdc.gov)

RNA helicase activity of Mtr4p is critical for biological functions of the enzyme, but the molecular basis for RNA unwinding is not understood. (nature.com)
The current gold-standard molecular diagnosis for COVID-19 is based on a multi-step assay involving RNA-extraction and RT-PCR analysis for the detection of SARS-CoV-2. (medrxiv.org)
Molecular Mechanisms of RNA Targeting by Cas13-containing Type VI CRISPR-Cas Systems. (rochester.edu)
Since perturbation of normal homeostatic levels of SRC-3 is linked with common gynecological disorders diagnosed in reproductive age women, this endometrial coregulator-along with its new molecular targets described here-may open novel clinical avenues in the diagnosis and/or treatment of a non-receptive endometrium, particularly in patients presenting non-aneuploid early pregnancy loss. (frontiersin.org)
Here we describe a molecular diagnostic approach for SARS-CoV-2 RNA detection using loop-mediated isothermal amplification (LAMP) and simple visual detection of amplification for potential use in rapid, field applications. (medrxiv.org)
To obtain mechanistic insights into how phosphorylation impacts on alternative splicing regulation we will combine available structural information for molecular modelling of ELAV multimerization and RNA binding to instruct experimental validation. (findaphd.com)
Further, this project will apply statistical analysis of data and we willl use molecular modelling of available structural information to make predictions how phosphorylations impacts on ELAV multimerization and RNA binding. (findaphd.com)

Since then, she has been working as a postodoc in Dr. Wassenegger's lab (RLP AgroScience GmbH, AlPlanta) and her main field of interest is RNA-interference (RNAi) and viroid biology in plants. (degruyter.com)

Scientists at Cold Spring Harbor Laboratory (CSHL) have identified a therapeutic RNA molecule that corrects the error in genetic processing that leads to familial dysautonomia, a rare inherited neurodegenerative disorder. (news-medical.net)
The RNA-like molecule--known as an antisense oligonucleotide (ASO)--was injected into mice whose cells carry the human IKBKAP mutation that causes FD. (news-medical.net)
Here, single-molecule high-resolution optical trapping measurements reveal that Mtr4p unwinds RNA duplexes by 3′-to-5′ translocation on the loading strand, that strand separation occurs in discrete steps of 6 base pairs and that a single Mtr4p molecule performs consecutive unwinding steps. (nature.com)
We can immediately and directly see the function of DNA (the molecule) as a carrier of information (the particular sequence of codons) to the ribosome level process. (uncommondescent.com)

Classic antimetabolite anticancer drug with chemical structure similar to endogenous intermediates or building blocks of DNA or RNA synthesis. (medscape.com)

Figure 2: Mtr4p unwinding rates versus tether tension and nucleotide composition for unwinding the 16-bp RNA hairpin. (nature.com)
New design strategies for ultra-specific CRISPR-Cas13a-based RNA-diagnostic tools with single-nucleotide mismatch sensitivity. (rochester.edu)

ELAV mediated 3'-end processing of ewg transcripts is evolutionary conserved despite sequence degeneration of the ELAV binding site. (findaphd.com)
It's now clear that gene transcripts can be constructed in various ways, yet many questions remain about the process. (the-scientist.com)

This step interferes with DNA and to a lesser degree with RNA synthesis. (medscape.com)
Doxorubicin is an anthracycline antibiotic that inhibits DNA and RNA synthesis. (medscape.com)

Researchers at Stanford University used such a method to produce amplified heterogeneous populations of RNA from limited quantities of cDNA. (genomicglossaries.com)
Amplified RNA synthesized from limited quantities of heterogeneous cDNA. (genomicglossaries.com)

Virus RNA, viral antigen, or infectious viral particles could be detected in postmortem tissues. (cdc.gov)
A new study by CDC is the first to show Zika virus RNA replicating in brain tissues of infants with microcephaly who later died and in placentas of women who suffered pregnancy losses after Zika infection during pregnancy. (cdc.gov)

Comparison of RNA-seq and microarray platforms for splice event detection using a cross-platform algorithm. (bioconductor.org)
Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. (rochester.edu)
Here we report a method to identify SARS-CoV-2 (COVID-19) virus RNA from purified RNA or cell lysis using loop-mediated isothermal amplification (LAMP) using a visual, colorimetric detection. (medrxiv.org)
Immunofluorescence study showed replication of SARS- tum is only seen in the later stage of infection (1-3,13), CoV in the cells derived from throat wash, demonstrating oral droplets generated during talking may represent an the possibility of developing a convenient antigen detection important route of transmission. (cdc.gov)

The nuclear localization signal (NLS) domain is located between residues 82 and 98 is of critical importance in ALS, and such is witnessed by the depletion or the mutations (notably A90V) of this domain, which cause loss-of-function from nucleus and promote aggregating, two processes very likely to conduct to TDP-43's toxic gain of function. (wikipedia.org)

Optimization of specific RNA knockdown in mammalian cells with CRISPR-Cas13. (rochester.edu)

Application of PAQR and KAPAC to RNA sequencing data from normal and tumor tissue samples uncovers motifs that can explain changes in cleavage and polyadenylation in specific cancers. (nih.gov)

Beginning in the 2017-2018 cycle, the HCV confirmatory antibody test (INNO-LIA) will be released in the HEPC dataset with HCV RNA and Genotype data. (cdc.gov)

Once considered an unimportant curiosity, Z-DNA is now recognized to provide an on-the-fly mechanism to regulate how an RNA transcript is edited. (the-scientist.com)
Using RT-PCR, 5'- and 3'-RACE approaches we clearly show that PDE10A transcript variants X3 and X5 are expressed in bovine testis as well as in primary spermatocytes and spermatids. (plos.org)

Exosomes secreted by the umbilical cord mesenchymal stromal cells (UMSCs) and circular RNAs (circRNAs) derived from exosomes have been shown to be associated with the progression of GDM-related complications. (hindawi.com)

Where do you find the 5' and 3' untranslated regions (UTRs)? (chiyuanye.com)

Bernstein, J., Patterson, D.N., Wilson, G.M. & Toth, E.A. Characterization of the essential activities of Saccharomyces cerevisiae Mtr4p, a 3′>5′ helicase partner of the nuclear exosome. (nature.com)
de la Cruz, J., Kressler, D., Tollervey, D. & Linder, P. Dob1p (Mtr4p) is a putative ATP-dependent RNA helicase required for the 3′ end formation of 5.8S rRNA in Saccharomyces cerevisiae . (nature.com)
The RNA helicase Mtr4p modulates polyadenylation in the TRAMP complex. (nature.com)
Johnson, S.J. & Jackson, R.N. Ski2-like RNA helicase structures: common themes and complex assemblies. (nature.com)

Long-recognized by scientists as the powerhouse of the cell involved in the role of energy production, mitochondria help regulate numerous cell biological processes. (stonybrook.edu)
When the nucleic acid spirals to the left, it takes on a zig-zag shape known as Z-DNA that appears to regulate RNA editing. (the-scientist.com)

NELF - negative elongation factor Binding to RNA pol II causes it to stably pause 30-50nt downstream of txn. (chiyuanye.com)

Members of the Pospiviroidae family replicate in the nucleus through double-stranded RNA intermediates, attracting the host RNA silencing machinery. (degruyter.com)

As a result, circRNAs exert important roles in numerous physiological and pathological processes [ 9 , 10 ]. (hindawi.com)

Now, researchers led by CSHL Professor Adrian Krainer, together with their collaborators at Ionis Pharmaceuticals, have used a synthetic piece of RNA to enable human and mouse cells to produce normal levels of IKAP, despite the presence of the disease mutation. (news-medical.net)

The software returns a data.frame with the detected alternative splicing events: gene name, type of event (cassette, alternative 3',…,etc), genomic position, statistical significance and increment of the percent spliced in ( \(\Delta \Psi\) ) for all the events. (bioconductor.org)

The lack of oxygen and glucose in the area of ischemic injury initiates a pathological cascade leading to blood-brain barrier (BBB) breakdown, inflammation, and neuronal cell death, a process that may be intercepted to limit stroke progression. (lu.se)

We describe the RNA sequence as information because that's how we (as humans) understand it. (uncommondescent.com)

Recently, non-coding RNAs have attracted more and more attention from researchers, as they have been found to affect a wider range of biological processes than previously believed. (mdpi.com)

The donor's serum sample was positive for West Nile Virus IgM, IgG, and neutralizing antibodies by serologic testing but negative for West Nile Virus RNA by nucleic acid amplification testing. (cdc.gov)

CDC scientists found Zika virus RNA persisted in fetal brains and in placentas for more than seven months after the pregnant women contracted Zika. (cdc.gov)

We exposed rats, cutaneously, to sodium lauryl sulfate (SLS, 1% and 10% aqueous solution), m- xylene (pure liquid), and d-limonene (pure liquid) for 1 h and measured transcriptional responses at the end of the exposure and 3 h later for comparison with untreated skin samples. (cdc.gov)

B) Immunohistochemistry analysis showed RusV antigen in cortical neurons and their processes. (cdc.gov)

Aging cells with weakened gene regulation spuriously transcribe RNAs, but their impact on health and longevity still needs to be examined. (the-scientist.com)

A special issue of Non-Coding RNA (ISSN 2311-553X). (mdpi.com)

We further show that RNA unwinding by Mtr4p requires interaction with upstream RNA duplex. (nature.com)

Novel therapeutic approaches that can be given beyond the limited time window of 3-4.5 h are still an unmet need to be addressed to improve stroke outcomes. (lu.se)

Studies report that circular RNAs (circRNAs) derived from exosomes may play a key role in GDM development. (hindawi.com)

Participants aged 6 years and older who were examined, had available surplus sera, and with specific laboratory findings based on the prior testing (HCV antibody screening reactive and HCV RNA negative) were eligible for inclusion for the Hepatitis C Confirmed Antibody (INNO-LIA) testing. (cdc.gov)

Anderson, J.T. & Wang, X. Nuclear RNA surveillance: no sign of substrates tailing off. (nature.com)
Schmidt, K. & Butler, J.S. Nuclear RNA surveillance: role of TRAMP in controlling exosome specificity. (nature.com)
Bernstein, J. & Toth, E.A. Yeast nuclear RNA processing. (nature.com)
van Hoof, A., Lennertz, P. & Parker, R. Yeast exosome mutants accumulate 3′-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs. (nature.com)
So the spices own is made up of RNA is called small nuclear RNA. (pearson.com)

We also found that SRC-3 depletion in cultured HESCs results in a significant attenuation in the induction of a wide-range of established biomarkers of decidualization, despite exposure of these cells to a deciduogenic stimulus and normal progesterone receptor expression. (frontiersin.org)

Enables RNA binding activity. (nih.gov)
RNA Binding and HEPN-Nuclease Activation Are Decoupled in CRISPR-Cas13a. (rochester.edu)

High-throughput RNA sequencing and bioinformatics analysis have led to the identification of several novel circRNAs that exhibit biological characteristics and regulatory functions [ 6 , 9 ]. (hindawi.com)

HCV-RNA positive samples were then tested with a line probe assay to determine the virus genotype from six genotypes, and subtype for genotype 1, the most prevalent genotype in the US. (cdc.gov)

After acquiring samples from patients, sequencing, running the algorithm to identify specific mutations, manufacturing the RNA, and delivering the vaccine to patients take about 6 weeks in total. (medscape.com)

Data from family studies confirmed familial clustering [3-5]. (springer.com)

A) Histopathologic analysis of cerebral sample from lion 3 indicated lymphohistiocytic meningoencephalitis and vasculitis. (cdc.gov)
In the first one, the complete analysis for junction arrays is described and the second one describes the analysis for RNA-Seq data. (bioconductor.org)
Cognizant of these limitations, we evaluated the extraction-free methods described in the literature and have developed an innovative, simplified and easy protocol employing limited reagents to extract RNA for subsequent RT-PCR analysis. (medrxiv.org)

Anatomy16

Organisms27

Diseases1

Chemicals and Drugs120

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes76

Information Science4

Health Care1

MRNA22

Polymerase13

Proteins11

Weighted gene1

MRNAs5

20221

Methylation3

Degradation5

Transcription4

Sequences4

Ribosomal2

Eukaryotic2

Molecules3

Highly conserved1

20231

MESSENGER RNA1

Recognition motifs2

Protein13

Genes4

Cellular7

Hepatitis C vir2

Gene expression6

Molecular7

Biology1

Molecule4

Endogenous1

Nucleotide2

Transcripts2

Synthesis2

CDNA2

Tissues2

Detection4

Residues1

Mammalian1

Cleavage1

20181

Transcript2

Stromal cells1

UTRs1

Helicase4

Regulate2

Elongation1

Intermediates1

Physiological1

Synthetic1

Genomic1

Neuronal1

Humans1

Biological processes1

Nucleic1

Fetal1

Transcriptional1

Antigen1

Regulation1

ISSN1

Upstream1

Therapeutic1

Exosomes1

Findings1

Nuclear5

Depletion1

Binding2

CircRNAs1

Assay1

Mutations1

Familial1

Analysis3