• However, it remains unclear what features of these genes dictate dynamic changes in their recognition and stepwise processing by spliceosome components to regulate splice site choice and splicing outcome. (biomedcentral.com)
  • The processes of animal cell growth, differentiation and infection by viruses result from the differential expression of specific genes, controlled primarily at the level of transcription. (rockefeller.edu)
  • To this end, our specific objectives are to determine the nature and mechanism of action of both the general transcription initiation factors that are commonly used by all genes and the gene- and cell type-specific factors that directly regulate target genes in response to various growth, developmental and viral stimuli. (rockefeller.edu)
  • The transcription of protein-coding genes involves RNA polymerase II and a number of common factors (TFIIA, -B, -D, -E, -F and -H) that form functional preinitiation complexes (on promoters) via an ordered assembly pathway. (rockefeller.edu)
  • Similarly, the transcription of small structural RNA genes (5S RNA, tRNA) by RNA polymerase III involves the assembly of common factors (TFIIIC and TFIIIB) into highly stable complexes via an ordered pathway. (rockefeller.edu)
  • To decipher the regulatory logic of adult pluripotent stem cells, we analyzed the chromatin organization of stem cell genes in the planarian Schmidtea mediterranea. (bvsalud.org)
  • We identify a special chromatin state of stem cell genes, which is distinct from that of tissue-specific genes and resembles constitutive genes. (bvsalud.org)
  • Where tissue-specific promoters have detectable transcription factor binding sites, the promoters of stem cell-specific genes instead have sequence features that broadly decrease nucleosome binding affinity. (bvsalud.org)
  • Genomic regions affected by the chromatin restructuring and host genes of exon usage differences show a strong enrichment for genes implicated in epilepsies, intellectual disability, and autism. (bvsalud.org)
  • Antioxidant genes such as ferritin are transcriptionally activated in oxidative stress via the antioxidant responsive element (ARE), to which nuclear factor-E2-related factor 2 (Nrf2) binds and activates transcription. (embl.de)
  • Numerous genes transcribed in oocytes are involved in multiple aspects of cell maintenance and protection, including metabolism, signal perception and transduction, RNA processing, cell cycle, defense against pathogens and DNA damage. (biomedcentral.com)
  • The major event of MBT was the activation of a large group of histones and other genes that modify chromatin structure preceding massive gene expression changes. (biomedcentral.com)
  • Similarly, the single-cell assay for transposase-accessible chromatin sequencing showed increased chromatin accessibility at genes associated with myeloid differentiation, including CD11b, CD11c, and IRF8. (nih.gov)
  • In eukaryotic cells, chromatin remodeling factors regulate chromatin structure by altering the assembly, disassembly, and relocalization of nucleosomes, thereby improving the local accessibility of transcription-related factors in their chromatin DNA, which further initiates or suppresses transcription of related genes. (creativebiomart.net)
  • Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)
  • Recruitment of multiple HATs to target promoters suggests an important role for chromatin remodeling in transcriptional activation of genes by steroid receptors. (drugbank.com)
  • The obvious next step is to integrate this information and learn how genes, proteins, and/or epigenetic factors influence the phenotype of a disease in context of the system. (rsc.org)
  • 1 Thecurrently available treatments use a combination of an HCV protease inhibitor withribavirin and PEGylated alpha interferon to disrupt virus replication, but the therapyis effective in only half of the people infected with HCV genotype 1 and even in thosepatients the efficacy is limited. (nature.com)
  • Replication processes permit copying a single DNA double helix into two DNA helices, which are divided into the daughter cells at mitosis. (wikipedia.org)
  • In G1 phase of the cell cycle, many of the DNA replication regulatory processes are initiated. (wikipedia.org)
  • The process of semiconservative replication for the site of DNA replication is a fork-like DNA structure, the replication fork, where the DNA helix is open, or unwound, exposing unpaired DNA nucleotides for recognition and base pairing for the incorporation of free nucleotides into double-stranded DNA. (wikipedia.org)
  • with the formation of the pre-replication complex (pre-RC) being a key intermediate in the replication initiation process. (wikipedia.org)
  • Association of the origin recognition complex (ORC) with a replication origin recruits the cell division cycle 6 protein (Cdc6) to form a platform for the loading of the minichromosome maintenance (Mcm 2-7) complex proteins, facilitated by the chromatin licensing and DNA replication factor 1 protein (Cdt1). (wikipedia.org)
  • Eukaryotic origins of replication control the formation of several protein complexes that lead to the assembly of two bidirectional DNA replication forks. (wikipedia.org)
  • The pre-RC formation involves the ordered assembly of many replication factors including the origin recognition complex (ORC), Cdc6 protein, Cdt1 protein, and minichromosome maintenance proteins (Mcm2-7). (wikipedia.org)
  • This transition involves the ordered assembly of additional replication factors to unwind the DNA and accumulate the multiple eukaryotic DNA polymerases around the unwound DNA. (wikipedia.org)
  • BACKGROUND: Anti-Silencing Factor 1 (ASF1) is a conserved H3-H4 histone chaperone involved in both Replication-Coupled and Replication-Independent (RI) nucleosomeassembly pathways. (ens-lyon.fr)
  • At DNA replication forks, ASF1 plays an important role in regulating the supply of H3.1/2 and H4 to the CAF-1 chromatin assembly complex. (ens-lyon.fr)
  • Chromatin is highly dynamic, a characteristic that is vital in regulating nuclear processes such as transcription and replication which require access to DNA. (biomedcentral.com)
  • The interactions of proteins with chromosomal DNA control a variety of cellular processes, including gene transcription, DNA packing, replication, recombination, and DNA repair. (psu.edu)
  • We have identified the proteins necessary to carry out the initial steps in strand invasion and the beginning of new DNA synthesis, which is significantly different from the normal process of replication. (brandeis.edu)
  • This is the process we have studied most intensively, but we are also interested in an alternative process known as break-induced replication (BIR), where only one end of a chromosome break locates a template sequence and assembles a complete replication fork and can copy sequences to the end of a chromosome, producing a nonreciprocal translocation. (brandeis.edu)
  • We have shown that there are differences between this repair-induced replication fork and the normal replication process, but there is much more work to do. (brandeis.edu)
  • The ORC-Cdc6 complex (product 1) assembles in step 1 around origin DNA and with the help of another replication initiator protein, Cdt1, it recruits the Mcm2-7 hexamer to the origin in step 2. (nature.com)
  • During DNA replication, obstacles to the progression of DNA replication forks can trigger repair by patching in the correct DNA sequence, using the second copy of that chromosome as a template - a process known as homologous recombination. (plos.org)
  • Violena Pietrobon, Sarah Lambert and colleagues investigated how the delicate balance between insufficient and excessive homologous recombination is maintained, identifying an evolutionarily conserved interplay between CAF-1 (a chromatin assembly factor) and RecQ-type helicases that helps to maintain genome stability in the face of replication stress. (plos.org)
  • The controlled assembly of replication forks is critical for genome stability. (imperial.ac.uk)
  • Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. (nih.gov)
  • The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. (nih.gov)
  • Using a sequential nucleoside analog incorporation assay, a high incidence of unidirectional replication fork movement is detected in testes-derived chromatin and DNA fibers. (sdbonline.org)
  • DNA replication occurs once and only once per cell cycle mainly regulated by DNA replication initiation factors in eukaryotic cells. (intechopen.com)
  • The pre-replication complex (pre-RC) assembly or the DNA replication licensing is the first step in DNA replication initiation, characterized by the sequential recruitment of ORCs, Cdc6, Cdt1 and MCMs to the DNA replication origins to form the pre-RC at the end of mitosis ( Bell and Dutta 2002 ). (intechopen.com)
  • Access to DNA is a prerequisite to the execution of essential cellular processes that include transcription, replication, chromosomal segregation, and DNA repair. (rockefeller.edu)
  • A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. (lookformedical.com)
  • This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. (lookformedical.com)
  • This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)
  • The human malaria parasite Plasmodium falciparum contains two nucleosome assembly proteins termed PfNapL and PfNapS. (biomedcentral.com)
  • Specific cloned DNA sequences can be assembled with proteins isolated from cellular extracts to provide details on the binding of each factor and its effect on the overall structure and activity of the complex. (psu.edu)
  • A hallmark of events observed during segmentation was the induction of multiple transcription factors, including a large group of homeobox proteins in pace with decay of a large fraction of maternal transcripts. (biomedcentral.com)
  • Moving lipids and proteins from the endoplasmic reticulum to the mitochondria (and vice versa) is a vital process, but the mechanism of transfer which occurs when regions of these organelles are in close contact is not known. (plos.org)
  • For the GAGA pioneer factor, experiments have recently shown that its ability to form larger oligomers is impacted by DNA binding, with a direct impact on its residence times and recruitment of additional proteins needed for transcription. (aps.org)
  • Here we implemented a reaction-diffusion model to simulate proteins binding and forming higher-order complexes on chromatin. (aps.org)
  • Histone modification will change in different biological processes, thereby providing a recognition marker and producing synergistic or antagonistic effects for the binding of other proteins to DNA. (creativebiomart.net)
  • Both proteins mediate protein-protein and protein-RNA interactions for cooperative RNA-binding during spliceosome assembly. (cipsm.de)
  • Furthermore, plasma membrane proteins and intracellular proteins can be released into the extracellular space by regulated or non-regulated processes. (cipsm.de)
  • In mice, rapamycin treatment increases expression of histone proteins and Wdfy3 transcription, and alters chromatin organisation in the small intestine, suggesting the mTORC1-histone axis is at least partially conserved in mammals and may offer new targets for anti-ageing interventions. (sdbonline.org)
  • How the proteins that regulate these processes function in the context of chromatin and its dynamic architectures is an intensive field of study. (rockefeller.edu)
  • Trans -acting splicing factors, pre-mRNA secondary structure and chromatin organization are now known to directly affect alternative splicing decisions [ 6 ]. (biomedcentral.com)
  • This genic organization makes pluripotency-related gene expression the default state in these cells, which is maintained by the activity of chromatin remodelers ISWI and SNF2 in the stem cells. (bvsalud.org)
  • NAPs participate in the assembly and disassembly of nucleosomes thus contributing to chromatin structure organization. (biomedcentral.com)
  • Elevated expression of H3/H4 in intestinal enterocytes in Drosophila alters chromatin organization, induces intestinal autophagy through transcriptional regulation, prevents age-related decline in the intestine. (sdbonline.org)
  • The 48 kDa subunit, RETINOBLASTOMA-BINDING PROTEIN 4, is also a component of several other protein complexes involved in chromatin remodeling. (lookformedical.com)
  • Co-transcriptional pre-mRNA processing relies on reversible phosphorylation of the carboxyl-terminal domain (CTD) of Rpb1, the largest subunit of RNA polymerase II (RNAP II). (cipsm.de)
  • Recognition of the 30-splice site is a key step in premRNA splicing and accomplished by a dynamic complex comprising splicing factor 1 (SF1) and the U2 snRNP auxiliary factor 65-kDa subunit (U2AF65). (cipsm.de)
  • Cleavage/polyadenylation specificity factor, A subunit, C-terminal [Interproscan]. (ntu.edu.sg)
  • Chromatin immunoprecipitation (ChIP) assay for CTCF was performed according to the Upstate protocol. (nih.gov)
  • BACKGROUND Nuclear hormone receptors are ligand-dependent transcription factors that require coactivators to regulate target gene expression. (sputnic-group.ru)
  • 1 SRC-3 phosphorylation and methylation have been shown to regulate such coactivator complex assembly. (sputnic-group.ru)
  • In this study, we demonstrate that ATP citrate lyase (Acly), which metabolizes citrate to generate cytosolic acetyl-CoA and is of clinical interest, can regulate chromatin accessibility to limit myeloid differentiation. (nih.gov)
  • Mechanistically, Acly deficiency altered chromatin accessibility and expression of multiple C/EBP family transcription factors known to regulate myeloid differentiation and cell metabolism, with increased Cebpe and decreased Cebpa and Cebpb. (nih.gov)
  • Acly inhibition thus can promote myelopoiesis through deprivation of acetyl-CoA and altered histone acetylome to regulate C/EBP transcription factor family activity for myeloid differentiation. (nih.gov)
  • The early Drosophila embryo is an attractive system to study chromatinassembly in a developmental context. (ens-lyon.fr)
  • Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex. (uci.edu)
  • Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA. (uci.edu)
  • Competition for PRPF8 availability alters the transcription-coupled splicing of RNAs in which weak 5′ splice sites predominate, enabling diversification of human gene expression during biological processes like mitosis. (biomedcentral.com)
  • Our studies are also directed toward the mechanisms by which these factors in turn are regulated in the various biological responses. (rockefeller.edu)
  • Thus, it is important to understand the fundamental biological processes of P. falciparum , which may provide avenues for the identification of new protein targets for development of new anti-malarials. (biomedcentral.com)
  • The mechanisms that underlie and dictate the different biological outcomes of E2F-1 activity have yet to be elucidated. (embl.de)
  • Our results define an exquisite level of precision in the reader-writer interplay that governs the biological outcome of E2F-1 activity. (embl.de)
  • 2,3 Accumulated results from both ex vivo and animal model studies indicate that SRC-3 plays important roles in many biological processes involving cell proliferation, cell migration, cell differentiation, somatic growth, sexual maturation, female reproductive function, and vasoprotection1. (sputnic-group.ru)
  • EF-P is present in all bacteria and orthologous to archaeal and eukaryotic initiation factor 5A, yet the biological function has so far remained enigmatic. (cipsm.de)
  • He concludes that mechanistic understanding of biological processes will lead to practical applications, and that the current rapid pace of progress is encouraging. (plos.org)
  • Here we will describe our current knowledge of the biological processes and mechanisms that can be considered bona fide epigenetic phenomena in Plasmodium biology, and attempt to distinguish them from those unlikely to involve epigenetic flow of information, even if chromatin changes occur. (plos.org)
  • In general, peak evaluation is intrinsically limited due to the lack of annotations for "true" binding sites, especially for experimental conditions in which biological signals might be shifted, changed, or depleted [ 1 ]. (biomedcentral.com)
  • Here, we review the ongoing development of accessibility measurements, we summarize the different molecular and structural mechanisms that shape the accessibility landscape, and we detail the many important biological functions that are linked to chromatin accessibility. (rockefeller.edu)
  • The precise regulations of pre-RC protein levels and assembly are effective ways to prevent reassembly of de novo MCM2-7 onto the replicated origins to re-license and re-replicate the genomic DNA in the subsequent phases of the same cell cycle ( Figure 1) . (intechopen.com)
  • The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. (lookformedical.com)
  • Most of the general factors (classes II and III) have been purified and individual subunits cloned for further structural and functional studies. (rockefeller.edu)
  • In the present study, the structural basis of nucleosome assembly activity in P. falciparum was addressed by determining and analysing the crystal structure of smaller NAP in the parasite called PfNapS. (biomedcentral.com)
  • A histone chaperone that facilitates nucleosome assembly by mediating the formation of the histone octamer and its transfer to DNA. (lookformedical.com)
  • ASF1 also provides H3.3-H4 dimers to HIRA and DAXX chaperones for RI nucleosome assembly. (ens-lyon.fr)
  • The repeating unit of chromatin is the nucleosome, formed from two histone H2A-H2B dimers and one histone H3-H4 tetramer around which 147 bp of DNA are wrapped [ 1 ]. (biomedcentral.com)
  • A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
  • Translation elongation factor P (EF-P) is critical for virulence in bacteria. (cipsm.de)
  • Recruitment of coactivators and HATs by the liganded PR in vivo may result in (i) remodeling of transcriptionally repressed chromatin to facilitate assembly and (ii) enhanced stabilization of the preinitiation complex by the activation functions of coactivators and the liganded PR itself. (drugbank.com)
  • Additional mechanisms that may control DNA accessibility in vivo include chromatin compaction and phase separation - processes that are beginning to be understood. (rockefeller.edu)
  • Chromatin target of Prmt1 (Chtop) is a vertebrate-specific chromatin-bound protein that plays an important role in transcriptional regulation. (embl.de)
  • The mechanisms effecting establishment, maintenance, and modification of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA. (lookformedical.com)
  • It is mainly manifested in the reduction of whole-genome DNA methylation level, hypermethylation of the promoter region, changes in specific histone modification, abnormal chromatin conformation, changes in non-coding RNA (ncRNA) molecules, etc . (creativebiomart.net)
  • In the study of Ras-induced tumor development, the importance of chromatin remodeling changes in cancer is also evident, with the observation that chromatin conformation is more open in early-developing tumors and more accessible in late-stage tumor development. (creativebiomart.net)
  • A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)
  • Collectively, these results suggest that targeted histone acetylation by recruited HAT cofactors and histone deacetylation are important factors affecting PR transactivation. (drugbank.com)
  • The formation of a diploid zygote begins with the unique, genome-wide RI assembly of paternal chromatin following sperm protamine eviction. (ens-lyon.fr)
  • Transcription factors (TFs) drive gene expression programs that shape specific phenotypes [ 3 ], and are frequently dysregulated in cancer [ 4 ]. (aging-us.com)
  • This separation of tasks could optimize the rapid assembly of paternal chromatin within the gigantic volume of the egg cell.In contrast, ASF1 is surprisingly dispensable for the amplification of cleavage nuclei, although chromatin integrity is likely compromised in KD embryos. (ens-lyon.fr)
  • Mouse liver were crosslinked with 1% formaldehyde for 10min, and nuclei were purified using standard sucrose cushion protocol. (nih.gov)
  • By combining super-resolution microscopy and chromatin fiber analyses with proximity ligation assays on intact nuclei, old H3 was found to be preferentially incorporated by the leading strand, whereas newly synthesized H3 is enriched on the lagging strand. (sdbonline.org)
  • These various classes of cofactors, as well as others (p300/CBP, STAGA) implicated in chromatin template modifications, offer important new mechanisms for gene regulation. (rockefeller.edu)
  • Histone modifications related to both active and inhibitory chromatin can be observed during cancer progression, and these modifications can occur simultaneously. (creativebiomart.net)
  • A closely related debate around chromatin modifications is about causality: several histone PTMs correlate with specific transcriptional states, but in many cases they are not responsible for a transcriptional outcome but rather are a consequence of it [7] . (plos.org)
  • In some processes, chromatin modifications carry heritable regulatory information that is transmitted from mother to daughter cells, whereas in other cases, they are implicated in the execution of the information contained in the DNA sequence, or occur as a consequence of dynamic nuclear processes such as transcription. (plos.org)
  • In the last few years, chromatin modifications have been extensively studied in the malaria parasite Plasmodium falciparum (for recent reviews, see [9] - [13] ). (plos.org)
  • We will not judge the use of the term epigenetic in different situations, but will rather attempt to clarify the roles of chromatin-based modifications in the different processes. (plos.org)
  • In addition, using invitro methylation assay we identified 4 new substrates for PRMT6, extending the involvement of this enzyme to other cellular processes beyond its well-established role in gene expression regulation. (embl.de)
  • Ran is a Ras-family GTPase that plays critical roles in multiple cellular processes including nucleo-cytoplasmic transport, nuclear envelope assembly and mitotic spindle assembly. (nih.gov)
  • Hence, the debate about the use of the term epigenetics is a terminology issue that affects our understanding of how cellular processes are ultimately controlled. (plos.org)
  • Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. (cancerindex.org)
  • The heterogeneity of the disease requires improved knowledge of key processes involved in tumour initiation, development and maintenance for translation into truly personalised treatment. (lu.se)
  • Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. (embl.de)
  • In S. cerevisiae , origin licensing occurs by the assembly during G1 phase of a pre-replicative complex (pre-RC) at each potential origin. (nature.com)
  • The limitations of current therapeutics primarily reflect incomplete understanding of the complex molecular signaling processes contributing to the heterogeneous nature of the disease [ 1 ]. (aging-us.com)
  • Much of our current work concerns the RanBP2 complex, which consists of RanBP2 (a large nucleoporin that is also known as Nup358), SUMO-1-conjugated RanGAP1 (the activating protein for the Ran GTPase), and Ubc9 (the conjugating enzyme for the SUMO family of ubiquitin-like modifiers). (nih.gov)
  • In general, a more aggressive behaviour is usually found in genetically unstable neoplasias with a higher number of genetic or epigenetic changes, which on the other hand, provoke a more complex chromatin rearrangement. (biomedcentral.com)
  • Furthermore, PR transactivation was repressed by recruiting HD1 into the PR-DNA complex by fusing HD1 to a PR ligand-binding domain-interacting portion of SRC-1. (drugbank.com)
  • Many processes in parasite biology involve changes at the chromatin level, including regulation of transcription along a complex life cycle, delimitation of functional elements in the genome, and antigenic variation. (plos.org)
  • Peak evaluation is complex, as gene expression regulation involves interactions between combinatorial transcription factor binding sites and chromatin states. (biomedcentral.com)
  • The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
  • This process allows for the high-fidelity passage of hereditary/genetic information from parental cell to daughter cell and is thus essential to all organisms. (wikipedia.org)
  • Sequential assembly of the human spliceosome on RNA transcripts regulates splicing across the human transcriptome. (biomedcentral.com)
  • The TAL1/SCL transcription factor regulates cell cycle progression and proliferation in differentiating murine bone marrow monocyte precursors. (nih.gov)
  • To test our hypothesis that histone H4R3 and H3R17 methylation regulates ferritin transcription, H4R3 and H3R17 protein arginine (R) methyltransferases 1 and 4 (PRMT1 and PRMT4) were investigated. (embl.de)
  • 2022 Dec 1;6(12):837-850. (nih.gov)
  • We demonstrate that HNRNPU deficiency leads to chromatin remodeling of A/B compartments, and transcriptional rewiring, partly by impacting exon inclusion during mRNA processing. (bvsalud.org)
  • We have shown that the invasion of DNA strands into a donor template region requires the action of the chromatin remodeling protein Rad54 that enables the recombination machinery to gain access to "closed" regions of DNA. (brandeis.edu)
  • SRC-3 interacts with nuclear receptors and certain other transcription factors, recruits histone acetyltransferases and methyltransferases for chromatin remodeling and facilitates target gene transcription. (sputnic-group.ru)
  • Tumor thickness, Clark's level, mitotic rate, nuclear area and fractal dimension were significant risk factors in univariate Cox regressions. (biomedcentral.com)
  • Interestingly, patients with HGPS do not develop other disease processes associated with aging, such as increased tumor formation, cataract development, or senility. (medscape.com)
  • [ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)
  • Stepwise sequential assembly of spliceosome components on these pre-mRNA motifs executes splicing reactions. (biomedcentral.com)
  • These processes are linked, as metabolites provide essential substrates for epigenetic marks. (nih.gov)
  • During the process of histone PTM, the enzymes responsible for addition or removal of these epigenetic markers are usually referred to as "writers" and "erasers", respectively. (creativebiomart.net)
  • Recruitment of 53BP1 to chromatin flanking double strand breaks (DSBs) requires γH2AX/MDC1/RNF8-dependent ubiquitination of chromatin and interaction of 53BP1 with histone H4 methylated on lysine 20 (H4K20me). (cipsm.de)
  • In asf1 KD embryos, HIRA localizes to the male nucleus but is only capable of limited and insufficient chromatin assembly. (ens-lyon.fr)
  • This process takes place in the G1 stage of the cell cycle. (wikipedia.org)
  • However, impaired splicing affects only a subset of human transcripts, enriched for mitotic cell cycle factors, leading to mitotic arrest. (biomedcentral.com)
  • In contrast, cyclin A binding to E2F-1 impedes PRMT1 methylation and augments PRMT5 methylation, thus ensuring that E2F-1 is locked into its cell-cycle progression mode. (embl.de)
  • Failure to do this results in cell death, showing that the process is essential for life. (plos.org)
  • We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)
  • Changes in dietary fatty acids, specifically the polyunsaturated fatty acids of the ω-3 and ω-6 families and some derived eicosanoids from lipoxygenases, cyclooxygenases, and cytochrome P-450, seem to control the activity of transcription factor families involved in cancer cell proliferation or cell death. (springer.com)
  • Hat1 is implicated in chromatin assembly and DNA repair but its role in cell functions is not clearly elucidated. (sdbonline.org)
  • The cell cycle is the process of accurate self-reproduction and proliferation of a cell. (intechopen.com)
  • As a transcription factor whose expression is increased by DNA damage, p53 blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
  • Our broad objectives are to understand the specific regulatory events that control these processes, as well as more fundamental aspects of transcription activation and repression mechanisms. (rockefeller.edu)
  • Some of the general factors have been shown to be direct targets for specific regulatory factors, while more recent studies have implicated a number of general and gene-specific cofactors in the function of gene-specific activators. (rockefeller.edu)
  • DNA binding factors shape the mouse methylome at distal regulatory regions [ChIP-seq]. (nih.gov)
  • Their regulation may be carried out either through direct binding to DNA as peroxisome proliferator-activated receptors or via modulation in an indirect manner of signaling pathway molecules (e.g., protein kinase C) and other transcription factors (nuclear factor kappa B and sterol regulatory element binding protein). (springer.com)
  • Using an in vitro transcription assay, we found that progesterone receptor (PR)-driven transcription is inhibited by a dominant negative PR ligand-binding domain-interacting region of SRC-1A, indicating that SRC-1A is required for actual transcriptional processes. (drugbank.com)
  • Here we show that the human PR also interacts with p300/CBP-associated factor in vitro. (drugbank.com)
  • It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. (cancerindex.org)
  • To study the collective dynamics and microscopic mechanisms that control this process, we need models that can capture the spatial and temporal dynamics of localization and assembly on and around DNA. (aps.org)
  • Knowledge of the mechanisms by which fatty acids control specific gene expression may identify important risk factors for cancer and provide insight into the development of new therapeutic strategies for a better management of whole body lipid metabolism. (springer.com)
  • 2007 M. musculus genome assembly (mm9) was used as a basis for all analyses. (nih.gov)
  • The core spliceosome component PRPF8 is essential for spliceosome assembly through its participation in ribonucleoprotein (RNP) complexes for splice-site recognition, branch-point formation and catalysis. (biomedcentral.com)
  • TAF9 is a TATA-binding protein associated factor (TAF) conserved from yeast to humans and shared by two transcription coactivator complexes, TFIID and SAGA. (thebiogrid.org)
  • Recombination between homologous sequences is a fundamentally important process both in meiosis and in mitotic cells. (brandeis.edu)
  • Disruption of RanBP2 association with kinetochores causes defective mitotic spindle assembly. (nih.gov)
  • The narrow diame- association with cellular and mitotic tubulin as ter and great length of single-walled carbon nano- well as the chromatin. (cdc.gov)
  • evm.model.tig00001501.4","tig00001501_g9219.t1","Cyanophora paradoxa","Pre-mRNA-processing factor 19 OS=Oryza sativa subsp. (ntu.edu.sg)
  • protein_coding" "Cz03g40300.t1","No alias","Chromochloris zofingiensis","Pre-mRNA processing factor 4 (PRP4)-like [Interproscan]. (ntu.edu.sg)
  • protein_coding" "Cz05g30030.t1","No alias","Chromochloris zofingiensis","mRNA splicing factor Cwf21 domain [Interproscan]. (ntu.edu.sg)
  • In addition, these coactivators also possess intrinsic histone acetyltransferase (HAT) activity and bind to each other and another HAT, p300/CBP-associated factor. (drugbank.com)