• This signaling pathway regulates various cellular processes and is involved in the growth of cells. (medlineplus.gov)
  • While results from mouse cells may not mirror cellular processes in humans , it is believed that the 145 height genes could be used in clinical settings to help find treatments for patients with skeletal dysplasia and other skeletal disorders. (medscape.com)
  • Here, we combined computational modeling with quantitative cell physiology to understand the dynamics of the plasma membrane (PM)-localized BR response pathway during the initiation of cellular responses in the epidermis of the Arabidopsis root tip that are be linked to cell elongation. (elifesciences.org)
  • Thus, we established the landscape of components and parameters for physiological processes potentially linked to cell elongation, a central process in plant development. (elifesciences.org)
  • In endochondral bone formation, parathyroid hormone-related protein (PTHrP)-expressing RZSCs are the source of growth plate chondrocytes, which give rise to bone-forming osteoblasts as well as bone marrow skeletal stem cells 1 . (nature.com)
  • The SAP49 protein is particularly important for the maturation of cells that build bones and cartilage (osteoblasts and chondrocytes). (medlineplus.gov)
  • Chondrocytes are the primary cell type involved in bone growth, multiplying and maturing in areas of tissue called growth plates , which sit near the ends of children's long bones and determine the child's eventual height . (medscape.com)
  • Although the effect of this protein shortage is unknown, researchers suspect that it disrupts spliceosome formation, which may impair mRNA processing and alter the activity of genes involved in the development of several parts of the body. (medlineplus.gov)
  • On the subject of integration and "complete solutions," GE Healthcare's ReadyToProcess™ product line covers all unit operations from media preparation and cell cultivation to protein purification. (genengnews.com)
  • Thus, crosstalk between periosteal and growth plate stem cells is essential for post-developmental skeletal growth. (nature.com)
  • In this system however, osteoblast differentiation of PSCs is selectively inhibited by Sp7 deficiency but PSCs are still present and may still play a role in skeletal growth. (nature.com)
  • This is a summary of the article "Genome-wide CRISPR screening of chondrocyte maturation newly implicates genes in skeletal growth and height-associated GWAS loci" in the Journal Cell Geonomics on April 14, 2023. (medscape.com)
  • Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (bioprocessintl.com)
  • An established biofilm structure comprises microbial cells and EPS, has a defined architecture, and provides an optimal environment for the exchange of genetic material between cells. (cdc.gov)
  • A biofilm is an assemblage of surface-associated microbial cells that is enclosed in an extracellular polymeric substance matrix. (cdc.gov)
  • Mammalian bone is formed through two distinct growth processes: endochondral and intramembranous bone formation 6 . (nature.com)
  • Mammalian cell culture reactors were adapted from chemical mixing tanks, which used shaft-and-impeller mixing. (genengnews.com)
  • While previous studies have characterized bacterial growth physiology at steady-state, a quantitative understanding of bacterial physiology in time-varying environments is lacking. (nature.com)
  • Here we develop a quantitative theory connecting bacterial growth and division rates to proteome allocation in time-varying nutrient environments. (nature.com)
  • To understand the dynamics of bacterial growth physiology and size control in dynamic nutrient environments, we have developed a coarse-grained proteome sector model which connects gene expression to growth rate and division control, and accurately predicts the cell-level E. coli response to nutrient perturbations in both exponential and stationary phase seen in experimental data 5 , 25 . (nature.com)
  • Heukelekian and Heller ( 1 ) observed the "bottle effect" for marine microorganisms, i.e., bacterial growth and activity were substantially enhanced by the incorporation of a surface to which these organisms could attach. (cdc.gov)
  • The CTS Rotea system offers exceptional cell recovery, flexible input and output volume capability, and high throughput, making it ideal for cell separation, concentration, washing, and more. (labmanager.com)
  • 3 ) used scanning and transmission electron microscopy to examine biofilms on trickling filters in a wastewater treatment plant and showed them to be composed of a variety of organisms (based on cell morphology). (cdc.gov)
  • VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
  • Many technologies are used to characterize biological products, manufacturing processes, and raw materials. (bioprocessintl.com)
  • The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
  • Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
  • Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
  • Moreover GE's recent announcement that it will acquire media specialist PAA Laboratories further strengthens its upstream process offerings. (genengnews.com)
  • A system designed for process flexibility allows users to optimize protocols for cell separation, washing, and concentration, and closed single-use kits enable sterile processing to facilitate scale-up to manufacturing. (labmanager.com)
  • Due to their reduced cell density, dead cells can be removed to optimize viability before delivery. (labmanager.com)
  • Bacteria reallocate their proteome to relieve metabolic or translational bottlenecks and increase growth rate under a given nutrient limitation 21 , but do not always allocate resources in order to optimize steady-state growth rate 22 . (nature.com)
  • Microorganisms, like bacteria and fungi, are becoming an emerging resource for the development of eco-sustainable plastic degradation and recycling processes. (frontiersin.org)
  • We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
  • Excessive proliferation of keratinocytes, abnormalities in the differentiation process, and continuous shedding of the thickened epidermis are characteristics of psoriasis (Figures 1 (a) and 1 (b)) [ 2 ]. (hindawi.com)
  • Two major thrusts in the last decade have dramatically impacted our understanding of biofilms: the utilization of the confocal laser scanning microscope to characterize biofilm ultrastructure, and an investigation of the genes involved in cell adhesion and biofilm formation. (cdc.gov)
  • Bacteria dynamically regulate cell size and growth to thrive in changing environments. (nature.com)
  • As a result, it remains unclear how cells sense changes in the environment and dynamically regulate division and growth in response. (nature.com)
  • Considering from the initial stage of blood clot formation to the last stage of newly-formed bone tissue remodeling, several cells and signaling molecules are involved which regulate (and are also regulated) during the development of this process. (bvsalud.org)
  • Beginning with harvest of material from a bioreactor, downstream processing removes or reduces contaminants to acceptable levels through several steps that typically include centrifugation, filtration, and/or chromatographic technologies. (bioprocessintl.com)
  • In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
  • PBS' U-shaped bioreactors employ a patented mixing technology, the Air-Wheel™, that exploits the fact that cell cultures must be oxygenated. (genengnews.com)
  • Furthermore, preclinical models of Wilms tumour, from cell lines to organoid cultures, have evolved. (bvsalud.org)
  • Angiogenesis is a balanced process controlled by pro- and anti-angiogenic molecules of which the regulation is not fully understood. (plos.org)
  • Angiogenesis is a balanced process controlled by pro- and anti-angiogenic molecules [3] . (plos.org)
  • Some molecules are involved in several stages of the process of alveolar repair, for example matrix metalloproteinases (MMPs) 2 , among others. (bvsalud.org)
  • Attachment is a complex process regulated by diverse characteristics of the growth medium, substratum, and cell surface. (cdc.gov)
  • For most of the history of microbiology, microorganisms have primarily been characterized as planktonic, freely suspended cells and described on the basis of their growth characteristics in nutritionally rich culture media. (cdc.gov)
  • We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
  • At distinct anatomical sites, bone tissue harbors multiple types of skeletal stem cells, which may independently supply osteogenic cells in a site-specific manner. (nature.com)
  • Rediscovery of a microbiologic phenomenon, first described by van Leeuwenhoek, that microorganisms attach to and grow universally on exposed surfaces led to studies that revealed surface-associated microorganisms (biofilms) exhibited a distinct phenotype with respect to gene transcription and growth rate. (cdc.gov)
  • Spliceosomes help process messenger RNA (mRNA), which is a chemical cousin of DNA that serves as a genetic blueprint for making proteins. (medlineplus.gov)
  • The model, consisting of ordinary differential equations, comprises the BR induced hyperpolarization of the PM, the acidification of the apoplast and subsequent cell wall swelling. (elifesciences.org)
  • Using CRISPR genome-editing technology to look at 600 million mouse cartilage cells , researchers began eliminating genes that influence how the cells proliferate and mature to determine which would influence skeletal changes . (medscape.com)
  • Researchers identified 145 genes in mouse cartilage cells that were similar to those seen in certain skeletal disorders that affect the growth development of bones, joints, and cartilage in human babies . (medscape.com)
  • Solutions for manufacturing cell therapy products must be designed for safety, process flexibility, and scale-up from research through commercial manufacturing. (labmanager.com)
  • The multipurpose Gibco™ Cell Therapy Systems (CTS™) Rotea™ Counterflow Centrifugation System offers unparalleled flexibility. (labmanager.com)
  • The compact footprint, process flexibility, and closed single?use kit allow the system to seamlessly scale with your process, from research through commercial manufacturing. (labmanager.com)
  • According to Christian Kaisermayer, Ph.D., project manager for cell culture at GE Healthcare Life Sciences the two significant drivers are process flexibility and complete solutions. (genengnews.com)
  • 4) remodeling stage, characterized by the substitution process of replacement of primary by secondary bone tissue (21 days after tooth extraction, in alveolus of rats) 1 . (bvsalud.org)
  • The CTS Rotea Counterflow Centrifugation System is a highly versatile tool that is suitable for cell separation, as well as buffer exchange and cell concentration between cell therapy processing steps. (labmanager.com)
  • Biofilm-associated cells can be differentiated from their suspended counterparts by generation of an extracellular polymeric substance (EPS) matrix, reduced growth rates, and the up- and down- regulation of specific genes. (cdc.gov)
  • Acetylation, which occurs normally in a cell, neutralizes the positive charges on the histone by changing amines into amides and decreases the ability of the histones to bind to DNA. (wikipedia.org)
  • Angiogenesis - the formation of new blood vessels - occurs during tissue growth and development, but also during wound healing and cancer [1] , [2] . (plos.org)
  • A counterflow centrifugation system designed for closed manufacturing reduces the risk of errors associated with manual cell processing to create a safer product. (labmanager.com)
  • However, it remains unclear whether there is functional crosstalk between these two types of skeletal stem cells. (nature.com)
  • Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
  • In such environments, cell size and growth are regulated by trade-offs between prioritization of biomass accumulation or division, resulting in decoupling of single-cell growth rate from population growth rate. (nature.com)
  • Among the different study models of bone repair, the model of bone repair in tooth socket has been very adequate because it enables a chronological evaluation of the repair process, detailing the main cellular events at each stage 31 . (bvsalud.org)
  • Please join us for a #BPIAskTheExpert on "Development of IEX Purification Process for Lentiviral Vectors. (bioprocessintl.com)
  • We apply our framework to well-known models of cell size control, such as the sizer, the timer, and the adder. (aps.org)
  • A greater understanding of biofilm processes should lead to novel, effective control strategies for biofilm control and a resulting improvement in patient management. (cdc.gov)
  • 1-1 Complexity of carcinogenic processes n rasng concerns about cancer causaton and ways to control the dsease n Europe. (who.int)
  • Disposables play an obvious role in scaleup, i.e., that form factors for smaller single-use cell culture vessels (e.g. (genengnews.com)
  • The secondary palate develops from the right and left palatal processes. (medscape.com)
  • A recent study identified cathepsin K ( Ctsk )-Cre expressing PSCs as a unique class of skeletal stem cells residing in the periosteum 3 . (nature.com)
  • We study the Stochastic Thermodynamics of cell growth and division using a theoretical framework based on branching processes with resetting. (aps.org)
  • The aim of this study was to evaluate comparatively the effect of fluoride (F) on the activity of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) involved in process of alveolar bone repair. (bvsalud.org)
  • Experts believe that most improvements in volumetric productivity-a key element in designing process scale-involve media and feed strategies. (genengnews.com)
  • Periosteal stem cells (PSCs) and growth plate resting zone stem cells (RZSCs) critically contribute to intramembranous and endochondral bone formation, respectively. (nature.com)
  • Here we show PSCs are not only required for intramembranous bone formation, but also for the growth plate maintenance and prolonged longitudinal bone growth. (nature.com)
  • Microscopically the process of bone repair was similar in all groups, being noted only a delay of the blood clot resorption and bone formation in the group of 50 ppm F. The expression for MMP-9 showed differences betweengroups only during the initial repair (7 days). (bvsalud.org)
  • The process of bone repair is an event finely regulated and characterized by different phases with predominance of specific cellular types, aiming at the formation of tissue in the affected area. (bvsalud.org)
  • We demonstrate that the competence of the root epidermal cells for the BR response predominantly depends on the amount and activity of H+-ATPases in the PM. The model further predicts that an influx of cations is required to compensate for the shift of positive charges caused by the apoplastic acidification. (elifesciences.org)
  • Ours results suggest an effect of fluoride on the activity of MMPs 2 and 9 at the initial period of alveolar repair which could be associated to the process of blood clot remission and delay in bone repair. (bvsalud.org)
  • According to Daniel Giroux, vp of R&D, duplicating the agitation mechanisms of small reactors in large systems is problematic without subjecting cells to significant shear damage. (genengnews.com)
  • Fluid-suspended cells are gently concentrated without ever forming a pellet in the cone and washed quickly and efficiently at unmatched recovery rates. (labmanager.com)
  • Failure of merging between the medial nasal and maxillary processes at 5 weeks' gestation, on one or both sides, results in CL. (medscape.com)
  • Furthermore, it is unclear if bacterial size modulation is simply a byproduct of the complex cellular response to changing environmental conditions, or if it serves as an adaptive mechanism employed by the cell to improve fitness in time-varying environments. (nature.com)
  • This property suggests an analogy between our model for cell growth and division and heat engines, and the introduction of a thermodynamic efficiency, which quantifies the conversion of one form of entropy production to another. (aps.org)
  • Cell division may be split into two sub-processes: branching, by which a given cell gives birth to an identical copy of itself, and resetting, by which some properties of the daughter cells (such as their size or age) are reset to new values following division. (aps.org)
  • Specifically, bacteria transiently prioritize biomass accumulation over production of division machinery during nutrient upshifts, while prioritizing division over growth during downshifts. (nature.com)