• The retinal morphology and ultrastructure of WNIN/GR-Ob and age-matched littermate lean rats were studied by microscopy and immunohistochemistry. (molvis.org)
  • Analysis of microscopy images is one of the most fundamental goals in the realm of immunohistochemistry. (hindawi.com)
  • These behavioral observations will be integrated with a quantitative 3D neuroanatomical analysis of termite brains through immunohistochemistry and confocal microscopy. (lu.se)
  • In addition, the in situ expression of VGLL3 in the GC tissues was determined by immunohistochemistry (IHC), and the patients were accordingly classified into the high and low expression groups. (researchgate.net)
  • Additionally, immunohistochemistry (IHC) was performed in multiple ovarian cancers, benign tumours and normal tissues to evaluate Gli2 and MMP-7 protein expression. (jcancer.org)
  • Since it is impractical to fuse a tag to every conceivable antigen-specific antibody, most immunolabeling processes use an indirect method of detection. (wikipedia.org)
  • Background Historically, immunohistochemistry (IHC) assays have been designed for simplicity of manual pathology, however, this is not always ideal to acquire the appropriate data for patient selection and outcomes. (flagshipbio.com)
  • Transfer tissue to a tissue cassette and store in 70% ethanol until tissue is processed and paraffin embedded. (ihcworld.com)
  • The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immunohistochemistry (IHC) staining method. (ac.ir)
  • This enables us to follow histopathological changes in course of neurodegenerative process (NDP) within the striatum and compare them with postmortem samples of human HD brains. (hindawi.com)
  • Brains and spinal cords were removed and processed for immunohistochemistry. (cdc.gov)
  • Immunolabeling is a biochemical process that enables the detection and localization of an antigen to a particular site within a cell, tissue, or organ. (wikipedia.org)
  • Discriminating between these different models requires biological information about the cells and tissue of the brain at the start of the folding process. (elifesciences.org)
  • This protocol ensures that mouse spine tissue will stay on the slide throughout the process. (ihcworld.com)
  • We can carry out the 'wet lab' aspects of your project and perform processing, tissue embedding, sectioning, and routine and specialised staining. (otago.ac.nz)
  • Using immunohistochemistry (IHC) to examine immune infiltrates in tissue biopsy samples to support exploratory investigations for immuno-oncology drug development is critical for understanding the abundance and spatial relationships of different immune cell types and how they may change with drug treatment. (flagshipbio.com)
  • Further studies are needed to establish the relationship between the initial process of resorption of the blood clot, and the involvement of MMPs 2 and 9 and its regulators/tissue inhibitors. (bvsalud.org)
  • The process of bone repair is an event finely regulated and characterized by different phases with predominance of specific cellular types, aiming at the formation of tissue in the affected area. (bvsalud.org)
  • 4) remodeling stage, characterized by the substitution process of replacement of primary by secondary bone tissue (21 days after tooth extraction, in alveolus of rats) 1 . (bvsalud.org)
  • Considering from the initial stage of blood clot formation to the last stage of newly-formed bone tissue remodeling, several cells and signaling molecules are involved which regulate (and are also regulated) during the development of this process. (bvsalud.org)
  • [ 4 ] Regardless of the underlying disease process, the universal pathophysiology is believed to be acute injury to lung parenchyma leading to chronic interstitial inflammation, tissue destruction, fibroblastic activation and proliferation, pulmonary fibrosis, and eventually architectural remodeling with honeycomb changes. (medscape.com)
  • Despite not having a standardized measurement parameter, the excess of neutrophil extracellular traps corresponds conception, manuscript to the severity of tissue damage observed in patients with respiratory tract infections, revealing the important prognostic role design, literature search, data of the neutrophil response and NETosis process in pulmonary infectious diseases. (bvsalud.org)
  • Since it was recognized that interstitial cells of Cajal express the gene product of c-kit, we performed immunohistochemistry for c-kit protein in ciliary muscle specimens of monkeys' eyes. (scielo.br)
  • Twenty-eight bones from 11 patients with RA undergoing orthopaedic surgery were analysed by quantitative and semi-quantitative immunohistochemistry. (bmj.com)
  • [ 1 ] Once a patient is diagnosed with ILD, progression to end-stage disease with honeycomb changes is based largely on the underlying disease process. (medscape.com)
  • During development, oligodendrocyte precursor cells (OPCs) differentiate into oligodendrocytes (OLs) that extend processes to contact multiple axons of CNS neurons. (jneurosci.org)
  • In the insect world, this essential navigational process is facilitated by specific neurons within a brain region known as the central complex (CX). (lu.se)
  • The most common application of immunostaining is immunohistochemistry, which is used to assist in the diagnosis of various diseases, including different types of cancer. (news-medical.net)
  • However, as the result of recent advancements in microscopic imaging technology and computational image processing techniques [ 1 ], there has been significant growth of research towards translational computational systems that can detect, analyze, classify, and quantify cell nuclei from microscopic images. (hindawi.com)
  • After extraction, the animals were euthanized at 7, 14, 21 and 30 days and the hemi-maxillae were collected for microscopic analysis (Hematoxylin and Eosin and immunohistochemistry for MMP-9) and zymography (MMP-2 and 9). (bvsalud.org)
  • The IEC is further updated on the basis of feedback Asbest study (Occupational exposure chaired by Professor Samar Al-Homoud, from IARC staff, disclosure requirements to chrysotile in workers in mines and supported by Dr Angeliki Kerasidou as set by the IARC/WHO Policy on Clinical processing facilities in Asbest, Russian vice-chair and assisted by Dr Chiara Trials Registration and Public Disclosure Federation). (who.int)
  • Whereas in the acute phase of the disease immunoregulatory processes prevail in the hippocampus and the cortex, we observed a strong activation of neurogenic processes in the hippocampal dentate gyrus, both by gene expression and immunohistology starting as early as 3 days after infection. (biomedcentral.com)
  • Another area in which it has made a significant impact is that of genetic study, where it has been used to determine the role of specific gene products, elucidating their function in vital biological processes. (news-medical.net)
  • This study intends to reveal the expression and functions of YAP during the CNV process. (molvis.org)
  • YAP expression was detected with western blotting and immunohistochemistry. (molvis.org)
  • Microscopically the process of bone repair was similar in all groups, being noted only a delay of the blood clot resorption and bone formation in the group of 50 ppm F. The expression for MMP-9 showed differences betweengroups only during the initial repair (7 days). (bvsalud.org)
  • The aims of this study were to evaluate the pretreatment PSMA expression on immunohistochemistry (IHC) and PSMA uptake on PET/CT imaging in mCRPC patients who underwent PSMA-RLT. (bvsalud.org)
  • We aimed to determine the significance of detecting expression of some antigens that might be pivotal to the process, namely CD44 and CD117, in patients with active pemphigus vulgaris. (who.int)
  • Bone immunohistochemistry is a relatively tricky procedure. (ihcworld.com)
  • The aim of this study was to evaluate comparatively the effect of fluoride (F) on the activity of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) involved in process of alveolar bone repair. (bvsalud.org)
  • Ours results suggest an effect of fluoride on the activity of MMPs 2 and 9 at the initial period of alveolar repair which could be associated to the process of blood clot remission and delay in bone repair. (bvsalud.org)
  • Among the different study models of bone repair, the model of bone repair in tooth socket has been very adequate because it enables a chronological evaluation of the repair process, detailing the main cellular events at each stage 31 . (bvsalud.org)
  • Understanding the processes of brain damage and repair following BM is a prerequisite for the development of new drugs that can preserve and restore neuronal function. (biomedcentral.com)
  • Global brain hypoxia-ischemia during cardiac arrest has a long-term impact on processing and transfer of sensory information by thalamic circuitry. (cdc.gov)
  • Remember to be wary of drying temperature, too high and some antigens (eg S100, 5D3, CMV) will be adversely affected (see Henwood, A., (2005) 'Effect of Slide Drying at 80°C on Immunohistochemistry' J Histotechnol 28(1):45-46). (utsouthwestern.edu)
  • We have previously described the histopathology reports (but not immunohistochemistry results) from four patients in this study. (bmj.com)
  • Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
  • En outre, il y avait une aug- mentation significative de la forme soluble du c-kit dans le sérum des patients atteints de pemphigus vulgaire actif par rapport aux témoins. (who.int)
  • Accurate results of cell nuclei segmentation are often adapted to a variety of applications such as the detection of cancerous cell nuclei and the observation of overlapping cellular events occurring during wound healing process in the human body. (hindawi.com)
  • Adapting to robust automated image processing techniques for primary tasks such as cell nuclei segmentation and quantification will not only prove to be time efficient for pathologists, but these techniques will also be capable of producing consistent results. (hindawi.com)
  • Computational models can be used to better understand this process. (springer.com)
  • In conjunction, ILK-deficient primary oligodendrocytes are defined by a failure in process extension and an inability to form myelin membrane upon axonal contact. (jneurosci.org)
  • Thus, targeting p75 NTR is a promising approach to reducing AD-related degenerative processes that have progressed beyond early stages. (plos.org)
  • Some molecules are involved in several stages of the process of alveolar repair, for example matrix metalloproteinases (MMPs) 2 , among others. (bvsalud.org)
  • The intracellular roles of NPM include a wide range of metabolic processes such as ribosome biogenesis, proliferation, cell cycle control, migration, DNA repair, non-programmed cell death, stress sensor ( 3 , 4 ). (frontiersin.org)
  • The process of myelination is controlled in part by cell extrinsic factors derived from the extracellular matrix (ECM). (jneurosci.org)
  • If the immunolabeling process is meant to reveal information about a cell or its substructures, the process is called immunocytochemistry. (wikipedia.org)
  • In recent years, numerous image processing techniques have been proposed for cell nuclei segmentation [ 2 ]. (hindawi.com)
  • A popular technique in the realm of image processing known as region growing is combined with a graph-cuts-based algorithm that incorporates Laplacian of Gaussian (LoG) filtering to detect cell nuclei [ 8 ]. (hindawi.com)
  • p53 is a transcription factor that participates in cell cycle checkpoint processes and apoptosis. (lu.se)
  • Furthermore, these methodologies present known challenges for design control processes and regulatory approval as companion diagnostics, preventing wide use beyond exploratory research settings. (flagshipbio.com)
  • However, the cellular and physical processes present during folding have not been defined. (elifesciences.org)
  • The committee also discussed the and advise the Director regarding the the Nouveau Centre, a new fast-track review, management, or disposal of old strategic development of the Biobank, Material Transfer Agreement process, a sample col ections in preparation for the both internal y and with external revised price structure for the biobank move to the Nouveau Centre. (who.int)