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  • tissues
  • The key elements of a successful islet isolation procedure are: (1) enzymatically digesting the tissues connecting the islets to the exocrine tissue, (2) separating islets from non-islet tissue, and (3) culturing isolated islets in an environment that maintains cell viability. (biomedcentral.com)
  • isolates
  • Oil seed protein isolates, in particular canola protein isolate , having a decreased phytic acid content is prepared by a procedure in which extraction of phytic acid from oil seed meal is inhibited during extraction of protein from the oil seed meal. (patentsencyclopedia.com)
  • Procedure for confirmation of non-O157 STEC isolates has been clarified with instructions to perform WGS and PFGE on all STEC isolates. (fda.gov)
  • Pulmonary vein isolation (PVI) is an option that electrically isolates 'hot spots' in the pulmonary veins that trigger AF. (intracare.co.nz)
  • laboratory
  • A detailed protocol is also included that describes a common method for rodent islet isolation that our laboratory uses to obtain viable and functional mouse islets for in vitro study of islet function, beta-cell physiology, and in vivo rodent islet transplantation. (biomedcentral.com)
  • This chapter establishes general policy and procedures pertaining to the management of laboratory services. (ihs.gov)
  • This revised chapter describes the policies, procedures, responsibilities and guidelines of the Laboratory Service. (ihs.gov)
  • The incumbent is responsible for coordination, formulation and development of IHS-wide laboratory policies, procedures and standards. (ihs.gov)
  • Specifically
  • I read a couple of more profound papers about CTAB-DNA-isolation-procedures (e.g. that one from Rogers & Bendich or Shivij, Rogers & Bendich), but I did not find any information why CTAB specifically binds to proteins at high salt concentrations, and to nucleic acids at low salt concentrations. (bio.net)
  • The procedure is conducted in an angiography suite ("cath lab") specifically set up for EP at Intra. (intracare.co.nz)
  • method
  • Herein, we report a convenient method for the isolation of gram quantities of homogeneous SGP from commercially available egg yolk powder using solid/liquid extraction and HILIC-HPLC purification. (uu.nl)
  • This rapid and inexpensive isolation and concentration method results in a MK recovery and purification that is comparable or better than other more elaborate techniques. (grantome.com)
  • In addition, we present a common method for the isolation of rodent islets used regularly in our islet isolation facility to consistently procure viable and functional islets for in vitro study of islet function, beta-cell physiology, and in vivo islet transplantation. (biomedcentral.com)
  • The method used for the isolation of large scale cosmid and plasmid DNA is an unpublished modification (16) of an alkaline lysis procedure (17,18) followed by equilibrium ultracentrifugation in cesium chloride-ethidium bromide gradients (1). (protocol-online.org)
  • provide
  • For patients who require both a PE and an airborne infection isolation room (AIIR), use an anteroom to ensure proper air balance relationships and provide independent exhaust of contaminated air to the outside, or place a HEPA filter in the exhaust duct. (psychiatryadvisor.com)
  • results
  • But only the CTAB-procedure (using a 2%CTAB-buffer with 2% CTAB, EDTA, Tris-HCl and beta-Mercaptoethenol) produced reproducible results. (bio.net)
  • Results: Sample collection techniques significantly affected the quantity of DNA for both, silica membrane based and phenol-chloroform isolations. (chemeurope.com)
  • series
  • The procedure, which involved a series of partitioning and column chromatography steps, was performed on 500 g of Mytilus edulis hepatopancreas tissue containing 14 mg of 1. (marine.ie)