• Native gel electrophoresis revealed a rapidly-modulated reciprocal relationship between the burden of unfolded proteins and BiP oligomers and slower equilibration between oligomers and inactive, covalently-modified BiP. (elifesciences.org)
  • The PCR products were detected by agarose gel electrophoresis (staining gel with ethidium bromide, followed by visualization under UV light). (cdc.gov)
  • A novel gel electrophoresis technique has reduced the time for the sample pretreatment of trace marker proteins derived from clinical samples from around 20 to 5 hours. (spectroscopyeurope.com)
  • Total cell lysates (50ng recombinant protein per lane) from rmSDF-1β, rhSDF-1β, rmSDF-1α and rhSDF-1α were resolved by 4-12% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with anti-CXCL12 (SDF-1β) (clone M4201G03). (biolegend.com)
  • Upon further chromatography analysis and acid hydrolysis of the modified proteins, Stark came to the conclusion that cyanate indeed reacts with amino and sulfhydryl groups, with the latter being a more rapid reaction. (wikipedia.org)
  • Proteins were visualized using a goat anti-rat IgG secondary antibody conjugated to HRP and chemiluminescence detection. (biolegend.com)
  • PCR amplicon of the pan- Orthohepevirus RT-PCR assay was extracted using the QIAquick gel extraction kit (Qiagen). (cdc.gov)
  • Jena Bioscience Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. (jenabioscience.com)
  • recommended agarose gel conc. (jenabioscience.com)
  • Equivalent amounts of radioactivity were loaded onto a 1% agarose denaturing gel. (mergullo.net)
  • It has GCMS liquid sample injection, Headspace injection, Solid Phase Micro Extraction (SPME) and DI Probe. (utm.my)
  • For headspace mode of analysis, it permits the detection of volatile substances in a liquid or solid sample and minimizes column contamination. (utm.my)
  • The Gel Loading Buffer provides highest convenience during routine handling and avoids commonly used gel staining procedures with Ethidium Bromide. (jenabioscience.com)
  • Creative Proteomics provides an activated carbon detection service platform to help you understand the chemical properties of the main ingredients in substances, ensure the quality and safety of substances, and comply with laws and regulations. (creative-proteomics.com)
  • Creative Proteomics has developed a rapid detection platform suitable for manufacturers, enterprises and government agencies, which can be used for the detection and analysis of activated carbon products testing analysis service. (creative-proteomics.com)
  • 6). Detection of specific nucleic acid sequences by hybridization a. mini-prep of plasmid DNA b. (ntu.edu.tw)
  • A simple fluorometric immunological method in combination with a magnetic concentration step was developed for rapid detection of target bacteria with high sensitivity and specificity in less than 2 h without enumeration. (docksci.com)
  • First of all, the magnetic Fe 3 O 4 NPs as core in the Fe 3 O 4 @SiO 2 NPs would facilitate the magnetic separation and recuperation from the detection system within the magnetic field. (hindawi.com)
  • High-throughput extraction, amplification, and detection (HEAD) of HCV-RNA in individual blood donations. (thermofisher.com)
  • Site-specific proteolysis and crosslinking were used to probe the architecture of oligomers formed by the endoplasmic reticulum (ER) Hsp70, BiP. (elifesciences.org)
  • In-situ fluorescent immunomagnetic multiplex detection of foodborne pathogens in very low numbers. (docksci.com)
  • Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. (enzolifesciences.com)
  • In addition to its high specificity, NIR-27 also shows lower cytotoxicity than indocyanine green, a nonspecific NIR probe widely used in clinic. (dovepress.com)
  • Most importantly, a qualified cancer NIR sensor should have high specificity for cancer-selective detection. (dovepress.com)
  • The magnetic Fe 3 O 4 @SiO 2 nanoparticles (NPs) functionalized with 8-chloroacetylaminoquinoline as a fluorescent sensor for detection and removal of Zn 2+ have been synthesized. (hindawi.com)
  • These characteristics suggest that NIR-27 is a promising prospect as a new NIR fluorescent sensor for sensitive cancer detection. (dovepress.com)
  • Biological molecules can be tagged with a fluorescent chemical group (fluorophore) by a simple chemical reaction , and the fluorescence of the tag enables sensitive and quantitative detection of the molecule. (newworldencyclopedia.org)
  • Conventional RT-PCR for HEV RNA detection in plasma, stool and liver biopsy samples was performed using primers listed in Technical Appendix Table 1. (cdc.gov)
  • A microfluidic device for preparing next generation DNA sequencing libraries and for automating other laboratory protocols that require one or more column chromatography steps. (thermofisher.com)
  • The complexity of food matrices, the wide variety of microorganisms therein, and the varying growth and replication traits of target pathogenic microorganisms that, when present, are often in much smaller numbers than the nonpathogenic microorganisms, pose significant challenges for pathogen detection. (docksci.com)
  • Primer & probe sequences, gene targets and product size of the 2 qRT-PCR assays specific for HEV-A and HEV-C are included in Technical Appendix Table 1. (cdc.gov)
  • So far, many selective smart-molecular fluorescent probes targeting Zn 2+ have been reported, that are mostly based on quinoline, fluorescein, benzazole, or fluorophores [ 6 - 10 ]. (hindawi.com)
  • Over the past decade, the modification of small molecular fluorescent probes on the surface of nanostructured materials has been attracting significant attention [ 15 - 18 ]. (hindawi.com)
  • Near-infrared (NIR) fluorescent sensors have emerged as promising molecular tools for cancer imaging and detection in living systems. (dovepress.com)
  • FITC-conjugated as well mainly because TRITC-conjugated secondary antibodies were from Molecular Probes and horseradish peroxidase-conjugated ones from SIGMA. (mergullo.net)
  • Noninvasive imaging with light photons represents an intriguing avenue to explore for cancer detection and diagnosis. (dovepress.com)
  • Semi-solid (gels) or solid formulations (freeze-dried tablets with controlled release) were also developed. (europa.eu)
  • The Shimadzu GCMS-TQ8040 is the first Triple Quadrupole with Smart Productivity for high efficiency sample throughput, Smart Operation for quick and easy method development, and Smart Performance for low detection limits and Scan/MRM. (utm.my)
  • The gel volume is affected by many environmental parameters including temperature, pH, ionic strength, and solvent composition. (uwaterloo.ca)
  • Using chromatography, Stark was able to detect a change in the amino acid sequence of ribonuclease, specifically the loss of lysine residues, in the presence of cyanate, and so he postulated that the cyanate facilitated the carbamylation of amino groups in the urea solution. (wikipedia.org)
  • In this work, 8-chloroacetylaminoquinoline (CAAQ) was synthesized and covalently linked to Fe 3 O 4 @SiO 2 NPs surface to establish an efficient system for the detection and removal of Zn 2+ (labeled as Fe 3 O 4 @SiO 2 -CAAQ). (hindawi.com)
  • This regulation requires enhanced detection of pathogens in both imported and domestically produced foods coupled with effective intervention methods to achieve food safety. (docksci.com)
  • comprehensive pathogen detection methods will enable implementation of effective strategies to avoid, or when needed intervene in, the distribution of foods that have been contaminated with foodborne pathogens. (docksci.com)