• Ovarian culture is an in-vitro process that allows for the investigation of the development, toxicology and pathology of the ovary. (wikipedia.org)
  • This method allows follicle-ovary interactions to be studied. (wikipedia.org)
  • The ovaries and follicles are dissected as above and then one follicle is placed in contact with one pole of a neonatal ovary on a plate. (wikipedia.org)
  • The follicle-ovary plate is cultured in follicle culture medium at 37 °C, 5% CO2 for up to 5 days. (wikipedia.org)
  • To facilitate differentiation between tissue origins the ovary and the follicle should be from different genetic sources. (wikipedia.org)
  • Ovarian cultures are useful to research as they can allow us to replicate systematic follicle development, periodical ovulation, and follicle atresia in an environment with modulated culture conditions.The ability of in vitro ovarian cultures to detect damage to the ovary and its specialised structures of the follicles and oocytes, allows for faster screening of potential developmental and/or reproductive toxicants. (wikipedia.org)
  • Culture of the whole ovary or ovarian fragments allows evaluation of various parameters in a controlled way and, therefore, has the potential for more complete reproductive toxicity studies. (wikipedia.org)
  • Our granulosa-like cells form ovary-like organoids (ovaroids) when aggregated with hPGCLCs, and recapitulate key ovarian phenotypes including support of germ cell maturation, follicle formation, and steroidogenesis. (biorxiv.org)
  • In mammals , the first part of oogenesis starts in the germinal epithelium , which gives rise to the development of ovarian follicles , the functional unit of the ovary . (wikipedia.org)
  • The number of antral follicles visible on ultrasound is indicative of the number of microscopic (and sound asleep) primordial follicles remaining in the ovary. (advancedfertility.com)
  • 5To evaluate whether 4 gram myoinositol and 400 mcg folic acid (MYO) therapy has any effects on ovarian stromal blood flow by using pulsed and color Doppler at 3 months follow-up period in polycystic ovary syndrome (PCOS). (infertilityschool.ru)
  • Only a few follicles in the human ovary survive to complete the cytodifferentiation process, with 99.9% dying by a programmed cell death mechanism called apoptosis. (glowm.com)
  • The normal ovary contains about 3-5 antral follicles. (roadlesstraveledstore.com)
  • An Antral Follicle Count (AFC), also known as a resting follicle count, tallies the number of primordial egg follicles within the ovary per menstrual cycle. (roadlesstraveledstore.com)
  • Selected candidates were further evaluated in ex vivo follicle/ovary culture assays and in vivo in mice and rats. (nih.gov)
  • The aims of the study were to investigate the influence of FSH and the ovary cutting treatments on the development of primordial follicles into preantral and antral follicles. (yarsi.ac.id)
  • All ovary groups were cultured in Dubellco's Modified Eagle Medium (DMEM) containing 5ug/ml insulin, 10ug/ml transferrin, 5ug/ml selenium (ITS), 5% FBS, 50ug/ml gentamycin with and without 100uIU/ml Follicle Stimulating Hormone (FSH). (yarsi.ac.id)
  • The ovary cutting treatment significantly increased the average number of collected preantral follicles. (yarsi.ac.id)
  • Development of follicles in the mammalian ovary. (yarsi.ac.id)
  • In summary, our results indicate that JAK signaling contributes to germline cyst breakdown and primordial follicle formation by regulating oocyte loss and pregranulosa cell proliferation in the fetal mouse ovary. (appliedbioinfo.com)
  • An increased electron density in the zona pellucida in some of the multilaminar primary and secondary follicles in the Polycystic ovary syndrome model was also observed at the ultrastructural level. (balkanmedicaljournal.org)
  • These results suggest that the decrease in the growth differentiation factor 9 and bone morphogenetic protein 15 expression initiated at the primary follicle stage effect the follicle development and zona pellucida structure and may cause subfertility or infertility in Polycystic ovary syndrome. (balkanmedicaljournal.org)
  • Ovarian insufficiency is a failure of the ovary to function adequately in a woman younger than 40 years, in its role either as an endocrine organ or as a reproductive organ. (medscape.com)
  • As defined by the World Health Organization, ovarian insufficiency can be caused by a primary disorder in the ovary or it can occur as a result of secondary causes. (medscape.com)
  • Ovarian insufficiency is considered primary if the ovary fails to function normally in response to appropriate gonadotropin stimulation provided by the hypothalamus and pituitary. (medscape.com)
  • In this review, we highlighted some of the main gene networks and regulatory mechanisms involved in the plasticity of sex-determining pathways that help to establish a functional ovary that can nurture the follicles to generate a good ovarian reserve, both in quantity and quality. (lidsen.com)
  • Polycystic Ovary Syndrome (PCOS) and Premature Ovarian Insufficiency (POI), were selected and briefly discussed in this review. (lidsen.com)
  • The most common human ovarian pathologies that cause infertility are Polycystic Ovary Syndrome (PCOS) and Premature Ovarian Insufficiency (POI), and both pathologies have been briefly discussed at the end of the review. (lidsen.com)
  • The act of ovarian stimulation is the ovary (Yin) being stimulated by Qi or IVF medications (Yang) for growth. (berkeley-acupuncture.com)
  • But the key is beginning with a truly, proper Yin environment for the ovary, follicles and oocytes (eggs) to flourish. (berkeley-acupuncture.com)
  • Yin is essentially the fluid nourishing the egg inside the follicle in the ovary and the cervical mucus in the uterus. (berkeley-acupuncture.com)
  • The mammalian ovary has all the molecular machinery required for the biosynthesis of melatonin and interestingly concentration of melatonin in the follicular fluid of pre-ovulatory follicles is much higher than circulatory melatonin even in humans. (jrhm.org)
  • We aimed to investigate the effects of different components of e-liquid on the ovary and compare the impact of low nicotine concentration e-liquids (LN e-liquids) and high nicotine concentration e-liquids (HN e-liquids) on ovarian toxicity. (tobaccoinduceddiseases.org)
  • Our findings indicate that nicotine has limited impact on the ovary, while flavoring, PG, and VG all cause ovarian damage including morphological damage, disruption of oxidative balance and promotion of apoptosis, with VG having the most significant effect. (tobaccoinduceddiseases.org)
  • Insulin signalling and glucose transport in the ovary and ovarian function during the ovarian cycle. (ijfs.ir)
  • At present no proven method can determine that a woman has no primordial follicles remaining in the ovary, so, in effect, this term is merely a construct (ie, a concept that cannot be proven). (medscape.com)
  • however, these terms are misleading because women with primary ovarian insufficiency do not always stop menstruating and their ovaries do not always completely stop functioning. (msdmanuals.com)
  • Thus, a diagnosis of primary ovarian insufficiency does not always mean that pregnancy is impossible. (msdmanuals.com)
  • Genetic disorders that confer a Y chromosome can also cause primary ovarian insufficiency. (msdmanuals.com)
  • Primary ovarian insufficiency (POI) causes female infertility by abolishing normal ovarian function. (nature.com)
  • Ovarian insufficiency is considered secondary if the hypothalamus and pituitary fail to provide appropriate gonadotropin stimulation. (medscape.com)
  • Primary ovarian insufficiency (POI) (premature ovarian failure, premature menopause, or early menopause) is a condition characterized by amenorrhea, hypoestrogenism, and elevated serum gonadotropin levels in women younger than 40 years. (medscape.com)
  • A karyotype should be performed as a part of the routine evaluation after the diagnosis of primary ovarian insufficiency is established. (medscape.com)
  • Ovarian ultrasonography can be useful in the workup of patients with primary ovarian insufficiency, as it will identify those women with multifollicular ovaries and suggest the diagnosis of either autoimmune oophoritis or 17-20 desmolase deficiency. (medscape.com)
  • Dividing the continuum of ovarian insufficiency into 4 clinical states is the authors' preferred method to facilitate explanation. (medscape.com)
  • Occult primary ovarian insufficiency presents as unexplained infertility in a patient with a normal basal serum follicle-stimulating hormone (FSH) level. (medscape.com)
  • Next on the continuum, biochemical primary ovarian insufficiency presents as unexplained infertility in patients with an elevated basal serum FSH level. (medscape.com)
  • Devenutto L, Quintana R, Quintana T. activation of ovarian cortex and autologous transplantation: A novel approach to primary ovarian insufficiency and diminished ovarian reserve. (revistareproduccion.org.ar)
  • There is much less data on this new approach in women with a natural ovarian insufficiency, which can be caused by different factors, including viral infection. (infertilityschool.ru)
  • In this case report this advanced methodology was used in a young patient suffering from ovarian insufficiency which was possibly associated with Epstein-Barr virus and infectious mononucleosis (glandular fever). (infertilityschool.ru)
  • Commonly used cancer therapies diminish the ovarian reserve, thus, resulting in primary ovarian insufficiency, which clinically presents as infertility. (koreamed.org)
  • Overt primary ovarian insufficiency is the clinical condition that has previously been referred to as premature ovarian failure or premature menopause. (medscape.com)
  • This method of culturing supports the growth of individual follicles from late pre-antral to pre-ovulatory stage. (wikipedia.org)
  • Late pre-antral follicles are identified as having a diameter of 180-200 µm and containing 2-3 layers of granulosa cells. (wikipedia.org)
  • Antral follicle counts by ultrasound are one of the best ovarian reserve tests that we currently have available. (advancedfertility.com)
  • What are Antral Follicles? (advancedfertility.com)
  • Antral follicles are small follicles (about 2-9 mm in diameter) that we can see - and measure and count - with ultrasound. (advancedfertility.com)
  • Antral follicles are also referred to as resting follicles. (advancedfertility.com)
  • Antral follicle counts (along with female age) are by far the best tool that we currently have for estimating ovarian reserve, the expected response to ovarian stimulating drugs, and the chance for successful pregnancy with in vitro fertilization. (advancedfertility.com)
  • When there are only a few antral follicles visible, there are far fewer eggs remaining as compared to when there are more antrals. (advancedfertility.com)
  • As women age, they have less eggs (primordial follicles) remaining and they have fewer antral follicles. (advancedfertility.com)
  • Antral follicle counts are a good predictor of the number of mature follicles that we will be able to stimulate in the woman's ovaries when we give injectable FSH medications that are used for in vitro fertilization. (advancedfertility.com)
  • When there are an average (or high) number of antral follicles, we tend to get a "good" response with many mature follicles. (advancedfertility.com)
  • When there are few antral follicles, we tend to get a poor response with few mature follicles. (advancedfertility.com)
  • When the number of antral follicles is intermediate, the response is not as predictable. (advancedfertility.com)
  • There are numerous antral follicles visible - marked with red. (advancedfertility.com)
  • 9 antral follicles are seen - marked with red. (advancedfertility.com)
  • Unfortunately, we do not live in a perfect world, and some ovaries have not yet read up on antral follicle counts to know how they are supposed to respond to stimulation. (advancedfertility.com)
  • Therefore, what we decide looks like 6 antral follicles, at another clinic might have been read as 4 or 8, etc. (advancedfertility.com)
  • The development of preantral and antral follicles is gonadotropin independent and gonadotropin dependent, respectively. (glowm.com)
  • What should antral follicle count be? (roadlesstraveledstore.com)
  • The antral follicles are the 2-10mm follicles within the ovarian stroma that represent the next waves of ovulation and can be visualized on a day 3 tranvaginal ultrasound. (roadlesstraveledstore.com)
  • In general, the more antral follicles we visualize, the better prognosis for the patient. (roadlesstraveledstore.com)
  • How can I increase my antral follicle count? (roadlesstraveledstore.com)
  • Supplementation with selenium and vitamin E as antioxidants can increase the level of AMH in women with OPOI and increase the number of antral follicles and ovarian volume. (roadlesstraveledstore.com)
  • Why is antral follicle count on Day 3? (roadlesstraveledstore.com)
  • The Basal Antral Follicle Count, along with the woman's age and Cycle Day 3 hormone levels, are used as indicators for estimating ovarian reserve and the woman's chances for pregnancy with in vitro fertilization. (roadlesstraveledstore.com)
  • Each antral follicle has the potential to develop into a viable egg for fertilization. (roadlesstraveledstore.com)
  • After additional cultured for 8 days, the percentage of preantral follicles developing into the antral follicles in DMEM with and without FSH were 26.7 + 5.7 and 11.7 + 2.9, respectively. (yarsi.ac.id)
  • In conclusion, the supplementation of FSH in the culture medium did not increase the number of preantral follicles, but significantly increased the number of antral follicles. (yarsi.ac.id)
  • Antral follicles develop in xenografted cryopreserved African elephant (Loxodonta africana) ovarian tissue. (yarsi.ac.id)
  • Factors influencing the rate of preantral and antral growth of mouse ovarian follicles in vitro. (yarsi.ac.id)
  • A study on rats demonstrated that the oocyte-derived GDF9-enhanced passage of primordial follicles to the primary stage was required for pre-antral follicle development (10). (balkanmedicaljournal.org)
  • In females with normal fertility, pre-antral ovarian theca cells respond to stimulation by inhibin B to provide androgen-based support for the developing follicle. (biomedcentral.com)
  • Although antral follicles were observed in her ovaries by ultrasound monitoring, she was amenorrhoeic with menopausal concentrations of follicle-stimulating hormone (FSH) and without mature follicles. (infertilityschool.ru)
  • Besides, a substantial decrease was detected in the number of ovarian antral, Graafian, and primordial follicles and also in the total number of corpus luteum following DHEA administration. (ijfs.ir)
  • Comparison of structural alterations in ovarian tissue between the PCOS+insulin and the PCOS groups displayed that insulin administration improved the total number of Graafian, primordial, and antral follicles and also corpus luteum. (ijfs.ir)
  • The AFC count is a major predictor of the ovarian reserve and could reflect the size of the remaining primordial follicle pool. (roadlesstraveledstore.com)
  • While existing hormone stimulation strategies targeted the growing follicles, the remaining dormant primordial follicles were ruled out from clinical use. (infertilityschool.ru)
  • Objective To compare the Follicular Output Rate (FORT) between corifollitropin alfa (CFA) and recombinant follicle-stimulating hormone (rFSH) during controlled ovarian stimulation (COS). (infertilityschool.ru)
  • After ovarian stimulation, it consists in collecting mature eggs (preferably) or even sometimes after in vitro maturation. (fertilys.org)
  • It usually requires ovarian stimulation. (fertilys.org)
  • It is thought that complications can be reduced by preferring mild stimulation in patients with high ovarian reserve. (ecerm.org)
  • It has been reported that 9%-24% of patients who undergo ovarian stimulation for IVF have poor ovarian response [ 12 , 13 ]. (ecerm.org)
  • This can be used to estimate the response to stimulation, for example, by In Vitro Fertilisation (IVF) but does not correlate with pregnancy success. (randoxhealth.com)
  • AMH data can be used by clinicians to determine the correct stimulation protocol and dosage to optimise the response to follicle stimulation in those with a low AMH result, and to avoid ovarian hyperstimulation syndrome (OHSS) in those with a high AMH result. (randoxhealth.com)
  • This technique can also be used to study possible applications of fertility treatments e.g. isolating oocytes from primordial ovarian follicles that could be used for fertilisation. (wikipedia.org)
  • Estrogenic endocrine disruptors may alter follicle formation/development and impair fertility or normal development of offspring. (hal.science)
  • At present, the only possibility to maintain fertility as well as hormonal cyclicity and endocrinological function is the cryopreservation and autologous re-transplantation of ovarian cortical fragments taken before chemotherapy or radiotherapy. (biomedcentral.com)
  • Direct-to-consumer (DTC) testing has become increasingly popular in recent years, with many companies offering tests that claim to evaluate fertility or ovarian reserve. (randoxhealth.com)
  • We performed a literature review to summarize data on the assessment of fertility potential and response to in vitro fertilization techniques on BRCA mutation carriers. (bmj.com)
  • While deteriorate as the potential mother ages, compromising, the receptivity of the uterus and the ovarian reserve of for example, the function of the chromosomal apparatus follicles can explain the 'maternal age effect' in part, it is or the ability to scavenge reactive oxygen species known that age-related decline of a female's fertility is resulting from mitochondrial reactions ( also rooted in the quality and developmental potential of her oocytes. (gotomydoctor.com)
  • Aging is associated with a decline in the number of ovarian follicles, menstrual irregularities, ovarian hormonal deficiency, anovulation, decreased fertility, and, finally, a complete and irreversible cessation of menses known as menopause, usually occurring at a mean age of 51 years. (medscape.com)
  • Melo P, Navarro C, Jones C, Coward K, Coleman L. The use of autologous platelet- rich plasma (PRP) versus no intervention in women with low ovarian reserve undergoing fertility treatment: a non-randomized interventional study. (revistareproduccion.org.ar)
  • In this review, we focused on the molecular mechanisms of ovarian sex determination and on understanding mutual cross-talks between central molecules in sex development which might impact fertility later in adult life. (lidsen.com)
  • The mature oocytes can be vitrified and stored for in vitro fertilization, which, along with ovarian tissue cryopreservation, is mostly practiced in young cancer patients to preserve their fertility. (infertilityschool.ru)
  • The devastating impact on fertility in such disorder can be successfully avoided by in vitro maturation of oocytes from excised ovarian tissue. (infertilityschool.ru)
  • The ovarian reserve is necessary for female fertility and endocrine health. (koreamed.org)
  • This system allows follicle growth and hormone production to be studied. (wikipedia.org)
  • Wells containing follicle culture medium (α-Minimal Essential Media, recombinant human follicle stimulating hormone, ascorbic acid and adult female mouse serum) is overlaid with sterilised silicon oil, which prevents medium evaporation. (wikipedia.org)
  • As follicles grow, they acquire receptors for luteinizing hormone, a pituitary hormone that reinitiates meiosis in the oocyte and causes ovulation of a fertilizable egg. (wikipedia.org)
  • Luteinizing hormone acts on receptors in the outer layers of granulosa cells of the follicle, causing a decrease in cyclic GMP in the granulosa cells. (wikipedia.org)
  • This hormone is strictly related to ovarian function. (infertilityschool.ru)
  • Specialized hypothalamic neurons secrete pulses of gonadotropin-releasing hormone (GnRH) into the portal blood vessels, which acts on the gonadotrophs to cause a pulsatile release of follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which act on ovarian follicle cells to control folliculogenesis. (glowm.com)
  • Assessment of the need for follicle stimulating hormone in early preantral mouse follicle culture in vitro. (yarsi.ac.id)
  • Regulation of mouse follicle development by follicle stimulating hormone in a three-dimensional in vitro culture system is dependent on follicle stage and dose. (yarsi.ac.id)
  • One of the key components of these tests is the measurement of Anti-Müllerian Hormone (AMH), which is produced by follicles in the ovaries and is known to reflect functional ovarian reserve. (randoxhealth.com)
  • AMH is a hormone produced by granulosa cells of small, growing ovarian follicles. (randoxhealth.com)
  • Hormones like follicle-stimulating hormone (FSH) and luteinizing hormone (LH) regulate the process of oocyte maturation. (randoxhealth.com)
  • This accelerated loss is poorly understood and is often associated with a small monotropic rise in the level of follicle-stimulating hormone (FSH) and decreased fecundity, 9 , 10 as well as an increased risk of aneuploidy. (ldysinger.com)
  • A disruption of this process may result in anovulation and ovarian steroid hormone deficiency. (medscape.com)
  • Typically, the human ovaries produce a single dominant follicle that results in a single ovulation each menstrual cycle (Fig. 1). (glowm.com)
  • In any given cycle, the dominant follicle must complete all the steps in folliculogenesis in a timely manner. (glowm.com)
  • 1 , 2 , 3 In each menstrual cycle, the dominant follicle that ovulates its egg originates from a primordial follicle that was recruited to initiate growth almost 1 year earlier (Fig. 2). (glowm.com)
  • The dominant follicle of the cycle appears to be selected from a cohort of class five follicles, and it requires about 20 days to develop to the ovulatory stage. (glowm.com)
  • The dominant follicle becomes a Graafian follicle, which releases the egg cell during ovulation. (randoxhealth.com)
  • Folliculogenesis and the menstrual cycle consists of the selection of the dominant follicle, stages of follicular development and ovulation and synchronised endometrial changes (4). (balkanmedicaljournal.org)
  • BMP15 is mitogenic for somatic cells and is known to induce granulosa cell proliferation and affect the choice of the dominant follicle as well as the formation of follicular atresia (12). (balkanmedicaljournal.org)
  • Ovarian development requires the combination of germ cells and their supporting somatic cells, known as granulosa cells. (biorxiv.org)
  • Whereas efficient protocols exist for generating human primordial germ cell-like cells (hPGCLCs) from human iPSCs, a method of generating granulosa cells has been elusive. (biorxiv.org)
  • [7] The cells that comprise the follicle, known as granulosa cells, are connected to each other by proteins known as gap junctions, that allow small molecules to pass between the cells. (wikipedia.org)
  • The remaining cells in the follicle wall ( i.e. granulosa, membrana and periantral, theca, the theca interna and externa) develop into the corpus luteum. (glowm.com)
  • Female mice lacking TAF(II)105 are viable but infertile because of a defect in folliculogenesis correlating with restricted expression of TAF(II)105 in the granulosa cells of the ovarian follicle. (appliedbioinfo.com)
  • AMH is the best reflection of a woman's functional ovarian reserve, or in other words, the pool of growing follicles in any given month. (randoxhealth.com)
  • The ovarian cortex samples were fixed with paraformaldehyde and histological analysis was done. (bvsalud.org)
  • The purpose of this practice is to conserve the primordial ovarian Follicles Located inside the ovaries, these tiny pockets of fluid contain the ova (or eggs). ">follicles , at the level of the ovarian cortex. (fertilys.org)
  • The aim of this study was to examine the effectiveness of Tumor Dissociation Enzyme (TDE) on the viability of follicles after digestion of fresh and cryopreserved ovarian cortex fragments (OCFs). (biomedcentral.com)
  • The enzymatic digestion of ovarian cortex with TDE provides recovery of a higher number of healthy preantral follicles in contrast to earlier described Liberase TM procedure. (biomedcentral.com)
  • Some reports show that it is possible to isolate immature oocytes from human ovarian tissue retrieved by a cortex biopsy or ovariectomy of non-stimulated ovaries and mature them in vitro. (infertilityschool.ru)
  • Therefore, a small biopsy of ovarian cortex tissue was performed using laparoscopy to retrieve immature oocytes. (infertilityschool.ru)
  • Follicles from different genetic sources can be co-cultured so that tissue origins can be differentiated within the co-culture. (wikipedia.org)
  • These new methods allow us to culture isolated ovarian follicles, embryos, ovaries (whole organ or only part of the tissue), and embryonic stem cells. (wikipedia.org)
  • Ovarian tissue transplantation has already led to the birth of hundreds of children around the world. (fertilys.org)
  • Freiman RN, et al 2001 reported the requirement of Tissue-Selective TBP-Associated Factor TAFII105 in Ovarian Development. (appliedbioinfo.com)
  • Successful isolation of preantral follicles from cryopreserved tissue is especially important in global practice: a large amount of ovarian tissues have been already conserved from patients with different types of cancers. (biomedcentral.com)
  • Four immature, germinal vesicle (GV) oocytes were found and removed from tissue, denuded mechanically by a pipette, and matured in vitro in a maturation medium with added FSH and hCG as well as in co-culture with cumulus cells, which were retrieved by their denudation. (infertilityschool.ru)
  • Three oocytes matured in vitro to the metaphase II (MII) stage and were vitrified for in vitro fertilization along with ovarian tissue cryopreservation. (infertilityschool.ru)
  • While much research has been conducted on e-cigarettes, it has been suggested that e-liquids themselves can damage tissues in vitro , inducing tissue oxidation and disrupting steroidogenesis 6 , 7 . (tobaccoinduceddiseases.org)
  • The total volume of ovarian tissue in mice receiving DHEA significantly declined compared to the control group. (ijfs.ir)
  • Detection of DNA damage in oocytes of small ovarian follicles following phosphoramide mustard exposures of cultured rodent ovaries in vitro. (hal.science)
  • At present research within the field of reproductive toxicology is principally carried out in vivo, however new culture methods have been developed with the aim of allowing ovarian follicles to be grown in vitro. (wikipedia.org)
  • Therefore, ovarian culture systems have become increasingly widely used in reproductive biology and toxicology. (wikipedia.org)
  • This model system will provide unique opportunities for studying human ovarian biology, and may enable the development of therapies for female reproductive health. (biorxiv.org)
  • It is a fact that while even basic reproductive information on alpacas is unavailable, the normal ovarian reserve of this species in comparison to other species is also unidentified. (bvsalud.org)
  • Women are born with a finite population of ovarian follicles, which are slowly depleted during their reproductive years until reproductive failure (menopause) occurs. (hal.science)
  • The primordial follicle (PF) assembly in the mouse occurs during the perinatal period, which determines the whole ovarian reserve in reproductive lifespan. (scite.ai)
  • The size of the primordial follicle pool determines the reproductive ability of female mammals. (appliedbioinfo.com)
  • Predictable menstrual cyclicity is a hallmark of healthy ovarian function during the reproductive years. (medscape.com)
  • Reduced quality and accelerated follicle loss with female reproductive aging - does decline in theca dehydroepiandrosterone (DHEA) underlie the problem? (biomedcentral.com)
  • Available: Herraiz S, Romeu M, Buigues A, Martínez S, Díaz-García C, Gómez- Seguí I, Martínez J, Pellicer N, Pellicer A. Autologous stem cell ovarian transplantation to increase reproductive potential in patients who are poor responders. (revistareproduccion.org.ar)
  • The eggs' freezing has greatly improved since the development of a vitrification technique (year 2000) which improved egg's survival (90%) and the chances of pregnancy after in vitro fertilization. (fertilys.org)
  • After in vitro fertilization, the embryos produced are generally vitrified at the Blastocyst The development stage of an embryo 5 to 7 days after fertilization. ">blastocyst stage (5 days of life). (fertilys.org)
  • In total, 789 patients who underwent OPU procedures for in vitro fertilization (IVF) were included in the study. (ecerm.org)
  • In vitro fertilization (IVF), in which fertilization takes place after mature eggs are collected from the ovaries, is a widely used method in the treatment of infertility [ 3 ]. (ecerm.org)
  • 11 Among women undergoing in vitro fertilization-embryo transfer in the United States, deliveries per oocyte retrieved decreased from 36.9 percent among women under the age of 35 years to 20.5 percent among women between the ages of 38 and 40 years and to 10.7 percent among women between the ages of 41 and 42 years. (ldysinger.com)
  • Each month, highly coordinated hormonal and ovarian morphological changes develop and release a mature oocyte that is ready for fertilization. (medscape.com)
  • Since successful in-vitro fertilization and embryo transfer demand optimal endometrial receptivity at the time of implantation a study was done to see if acupuncture could actually increase blood flow to the uterus. (berkeley-acupuncture.com)
  • Usage of melatonin during in vitro fertilization (IVF) procedures has been found to improve oocyte quality, survival, and fecundity. (jrhm.org)
  • Recognition that only a few follicles become dominant beautifully demonstrates the fundamental principle that folliculogenesis in mammals is a highly selective process. (glowm.com)
  • Folliculogenesis is the process in which a recruited primordial follicle grows and develops into a specialized graafian follicle with the potential to either ovulate its egg into the oviduct at mid-cycle to be fertilized or to die by atresia. (glowm.com)
  • During the course of folliculogenesis, growth is achieved by cell proliferation and formation of follicular fluid, whereas development involves cytodifferentiation of all the cells and tissues in the follicle. (glowm.com)
  • Although GnRH, FSH, and LH are critically important in regulating folliculogenesis, hormones and growth factors, which are themselves products of the follicle, can act locally to modulate (amplify or attenuate) FSH and LH action. (glowm.com)
  • It is believed that this local regulatory system plays an important role in the complex mechanisms governing the timing of folliculogenesis and whether a follicle becomes dominant or atretic. (glowm.com)
  • We designed an in vitro platform to screen and validate small molecules that can activate MISR2 signaling and suppress ovarian folliculogenesis. (nih.gov)
  • Here, we report that SP600125, CYC-116, gandotinib, and ruxolitinib can specifically inhibit primordial follicle activation and repress folliculogenesis by stimulating the MISR2 pathway. (nih.gov)
  • Differential FSH exposure in preantral follicle culture has marked effects on folliculogenesis and oocyte developmental competence. (yarsi.ac.id)
  • Ovarian folliculogenesis is organised by the interaction of extraovarian and intraovarian factors that coordinate the processes of oocyte growth and follicular development in the periovulatory period (5). (balkanmedicaljournal.org)
  • Although this level of plasticity is still found in the fully differentiated gonads, errors like mutations or epigenetic modifications impact ovarian development and, later, folliculogenesis, resulting in infertility. (lidsen.com)
  • The ovaries of young mice (19-23 days) are removed and halved, and follicles are identified under a microscope. (wikipedia.org)
  • Most in vitro toxicology studies use female mice and rat models. (wikipedia.org)
  • In adult mice, CPA also induced follicle loss in vivo. (hal.science)
  • In the current investigation, we administered ZEN orally to perinatal mice from 16.5 days post coitum (dpc) to postnatal day 3 (PD3), and single-cell RNA sequencing (scRNA-seq) was performed on PD0 and PD3 ovarian tissues in the offspring to check ZEN toxic to primordial follicle formation at the single cell level. (scite.ai)
  • Effect of follicle isolation technique on in vitro follicular development, oocyte maturation and embryo development in mice. (yarsi.ac.id)
  • JAK signaling regulates germline cyst breakdown and primordial follicle formation in mice. (appliedbioinfo.com)
  • 17 ]. It was demonstrated the real possibility for successful re-transplantation of follicles seeded with scaffolds to SCID-mice with subsequent pups born through natural mating. (biomedcentral.com)
  • 3 Although ovarian aging in mice has a hypothalamic component, this has not been found to be the case in humans. (ldysinger.com)
  • In GDF9 null mice, follicle development was arrested at the primary stage with no further growth (11). (balkanmedicaljournal.org)
  • Photomicrograph of ovulation shows the expanded egg-cumulus complex leaving the follicle through the stigma. (glowm.com)
  • From Blandau RJ: Growth of the ovarian follicle and ovulation. (glowm.com)
  • The LH surge initiates the luteinization of preovulatory follicles and causes hormonal and structural changes that ultimately lead to ovulation and the formation of corpora lutea. (appliedbioinfo.com)
  • factor(s) relies on gene transcription that oocytes largely Oocytes spend most of their time quiescent in primordial perform during follicular growth prior to ovulation, and follicles, mature over days or weeks during follicular the transcriptional activities of oocytes may be influ- growth and then become quiescent again near the enced by maternal age. (gotomydoctor.com)
  • BMP15 and GDF9 play a critical role in follicle development, oocyte maturation, ovulation and embryo development (8,9). (balkanmedicaljournal.org)
  • The mechanisms regulating follicle growth and development are under the control of changing concentrations of ligands ( i.e. hormones and growth factors). (glowm.com)
  • Maintenance of meiotic arrest also depends on the presence of a multilayered complex of cells, known as a follicle, that surrounds the oocyte. (wikipedia.org)
  • Later, the mature Graafian follicle ruptures, releasing the egg cell. (randoxhealth.com)
  • Using a model ovotoxic alkylating drug, cyclophosphamide (CPA), and its active metabolite, phosphoramide mustard (PM), we previously showed that PM (≥3μM) caused significant follicle loss in postnatal day 4 (PND4) mouse ovaries in vitro. (hal.science)
  • Cigarette smoking is linked to menopause occurring 1-4 yr earlier than with nonsmokers, and components of smoke, polycyclic aromatic hydrocarbons, can cause follicle depletion in rodents or in ovaries in vitro. (hal.science)
  • In an industrial setting, 2-bromopropane caused infertility in men and women, and it can induce ovarian follicle depletion in rats. (hal.science)
  • This might affect the ovarian reserve and lead to the diagnosis of infertility later in life. (lidsen.com)
  • This case report included a 27-year-old patient who attended our infertility clinic because of ovarian failure as a part of autoimmune polyendocrinopathy that occurred after Epstein-Barr virus infection, which has rarely been reported until now. (infertilityschool.ru)
  • ABSTRACT The development of preantral ovarian follicular (POF) culture systems with stem cells can reduce oxidative stress by means of the secretasomes released by these cells, which are small molecules composed of antioxidants, growth factors and chemokines. (ufpi.br)
  • Human primordial, primary and secondary ovarian follicles in long-term culture: effect of partial isolation. (yarsi.ac.id)
  • A reproducible two-step culture system for isolated primary mouse ovarian follicles as single functional units. (yarsi.ac.id)
  • Xenobiotic effects on ovarian preantral follicles. (hal.science)
  • Ionizing radiation reduces preantral follicle numbers in rodents and humans in a dose-dependent manner. (hal.science)
  • In this capacity, it survives the negative events that operate to destroy the other follicles by atresia. (glowm.com)
  • Atresia can occur in preantral class 1, 2, and 3 follicles. (glowm.com)
  • The subtle increase in the level of FSH is thought to increase atresia, which is coupled with an accelerated loss of follicles and a further increase in FSH, thus resulting in a positive-feedback loop. (ldysinger.com)
  • It has been hypothesized that there is a fixed window of some 13 years before menopause, during which accelerated ovarian atresia takes place. (ldysinger.com)
  • Lo que ya se sabe: se postula que el PRP podría ser beneficioso para retrasar la atresia del folículo y la degeneración de los ovocitos, además de promover el desarrollo de los folículos primordiales y primarios hasta el estadio preantral. (revistareproduccion.org.ar)
  • With depletion of follicle numbers, inhibin B is reduced with consequent reduction in theca DHEA. (biomedcentral.com)
  • Premature ovarian failure is the extreme state of complete primordial follicle depletion. (medscape.com)
  • Exposure of cultured PND4 mouse ovaries to 3 and 0.1μM PM induced significant losses of primordial and small primary follicles, respectively. (hal.science)
  • The normal follicles were measured and classified in primordial, transitional, primary and secondary stages. (bvsalud.org)
  • primary (6.10%) and secondary (0.37%) follicles were rarely found. (bvsalud.org)
  • The primary and secondary follicles were larger in diameter when compared with the primordial and transitional follicles. (bvsalud.org)
  • Practically, cycle regulation, chromosome stability and epigenetic F9 cells allow for the efficient metabolic labelling of the modification, in both mouse and human oocytes SILAC reference in vitro, overcoming the difficulty of directly labelling oocytes in vivo. (gotomydoctor.com)
  • Ovarian reserve parameters and IVF outcomes in 510 women with poor ovarian response (POR) treated with intraovarian injection of autologous platelet rich plasma (PRP). (revistareproduccion.org.ar)
  • What is new in the management of poor ovarian response in IVF? (revistareproduccion.org.ar)
  • In some cases, normal ovarian function may even return for a period of time. (medscape.com)
  • In vivo and in vitro studies have suggested that GDF9 and BMP15 contribute to the formation of the pathogenesis of PCOS (13-15). (balkanmedicaljournal.org)
  • It is possible because the basal lamina surrounding the follicles separates them from the surrounding ovarian stroma, blood vessels and nerves, which prevents the invasion of metastatic cells [ 14 ]. (biomedcentral.com)
  • This chapter considers what is known about the process underlying the expression of the structural and functional organization of developing follicles and how they are controlled. (glowm.com)
  • The follicles are dysfunctional (as occurs in autoimmune ovarian dysfunction). (msdmanuals.com)
  • Our results show that Epstein-Barr infection is possibly associated with autoimmune ovarian failure. (infertilityschool.ru)
  • This locally produced melatonin has been shown to modulate various pathways governing ovarian steroidogenesis. (jrhm.org)
  • Ovarian cortical strips had been Handshake antibiotic stewardship purchased from mares (N Equates to 15) via the Biopsy Pick-Up approach in the propagation time of year. (sykpathway.com)
  • These disorders, which are usually evident by age 35, increase risk of ovarian germ cell cancer. (msdmanuals.com)
  • For this reason, the authors prefer not to use the term premature ovarian failure (POF). (medscape.com)
  • The mammalian oocyte orchestrates the rate of ovarian follicular development. (yarsi.ac.id)
  • Materiales y métodos: se incluyeron 61 pacientes bajas respondedoras que realizaron inyección de PRP entre octubre 2021 a marzo 2022 en nuestra institución, comparando la función ovárica previa y posterior a la misma. (revistareproduccion.org.ar)
  • Chemotherapeutic agents, such as alkylating drugs and cisplatin, also cause loss of preantral ovarian follicles. (hal.science)