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  • Hematopoiesis
  • Immature stem cells showed a non-clonal pattern of X-inactivation and were detectable at diagnosis in the presence of clonal hematopoiesis. (haematologica.org)
  • The studies described herein examine the role in hematopoiesis of Osteopontin (Opn), a multidomain, phosphorylated glycoprotein, synthesized by osteoblasts, with well-described roles in cell adhesion, inflammatory responses, angiogenesis, and tumor metastasis. (edu.au)
  • A first wave of hematopoiesis follows MHE colony emergence and is predominated by primitive erythropoiesis characterized by a brilliant red hemoglobinization, CD71/CD325a (glycophorin A) expression, and exclusively embryonic/fetal hemoglobin expression. (bloodjournal.org)
  • Lin - Sca + Kit + (LSK + ) cells and Lin - Sca + Kit - (LSK - ) cells display differential abilities to reconstitute hematopoiesis, and the latter is a more mature cell type than the former, only able to support hematopoiesis in the short term. (bloodjournal.org)
  • Milner LA, Bigas A. Notch as a mediator of cell fate determination in hematopoiesis: evidence and speculation. (springer.com)
  • stem cell-
  • Regulation of stem cell-niche interactions influences the entire lymphohematopoietic system and ultimately the organism, making characterization of these pathways fundamental to the pursuit of novel therapeutic interventions involving adult stem cells. (grantome.com)
  • Therefore, OPN is a negative regulatory element of the stem cell niche that limits the size of the stem cell pool and may provide a mechanism for restricting excess stem cell expansion under conditions of niche stimulation. (rupress.org)
  • The stem cell niche is a specialized microenvironment that houses and regulates the stem cell pool. (rupress.org)
  • endothelial
  • 7 - 17 Interestingly, in both mouse and humans, there is compelling evidence that both yolk sac- and AGM-derived HSPCs are the direct progeny of a bipotential hemogenic endothelial cell or hemangioblast. (bloodjournal.org)
  • Publications] Zhang X-Q: 'Stromal cells expressing ephrin-B2 promote the growth and sprouting of ephrin-B2^+ endothelial cells'Blood. (nii.ac.jp)
  • Publications] Ohneda O: 'ALCAM(CD166):its role in hematopoietic and endothelial development'Blood. (nii.ac.jp)
  • Publications] Yokomizo T: 'Requirement of Runx1/AML1/PEBP2B for the generation of hematopoietic and endothelial cells'Genes to Cells. (nii.ac.jp)
  • By using adenovirus-mediated gene transfer, we show that overexpression of AP-1 proteins directly causes coinduction of gene expression of an adhesion molecule, intercellular adhesion molecule-1 (ICAM-1), and a chemokine, monocyte chemoattractant protein-1 (MCP-1), in human vascular endothelial cells (ECs). (ahajournals.org)
  • Endothelial cell (EC) perturbation or activation can be caused by various mechanical, chemical, or biological stimuli. (ahajournals.org)
  • mice
  • We performed a genetic screen for micro-RNAs that are differentially expressed between LSK, LS(-)K(+), erythroid and myeloid cells isolated from C57BL/6 and DBA/2 mice. (stemcellbiology.nl)
  • To functionally characterize these microRNAs, we overexpressed the entire miR-cluster 99b/let-7e/125a and miR-125a alone in BM cells from C57BL/6 mice. (stemcellbiology.nl)
  • Furthermore, infusion of normal bone marrow cells rescued mice from death induced by loss of THOC5/Fms interacting protein. (biomedcentral.com)
  • Development and aging of primitive hematopoietic stem cells in BALB/cBy mice. (jax.org)
  • Concentrations of HSC that engrafted and functioned were similar among 16-day fetal liver cells and bone marrow cells (BMC) from 3-month and 25 to 28-month-old adult mice. (jax.org)
  • Working in mouse models, it was also shown that whilst mice lacking p21 were healthy, spontaneous tumours developed and G1 checkpoint control was compromised in cells derived from these mice. (wikipedia.org)
  • Bedelbaeva K, Snyder A, Gourevitch D, Clark L, Zhang X-M, Leferovich J, Cheverud JM, Lieberman P, Heber-Katz E. Lack of p21 expression links cell cycle control and appendage regeneration in mice . (wikipedia.org)
  • Using studies that combine OPN-deficient mice and exogenous OPN, we demonstrate that OPN modifies primitive hematopoietic cell number and function in a stem cell-nonautonomous manner. (rupress.org)
  • By applying a similar strategy of transplanting FACS sorted single cells from solid tumors into immunodeficient mice, a small subpopulation of tumor-initiating cells has been identified from a variety of solid tumors, including breast (see review by Clarke and colleagues ref. 6 ). (aacrjournals.org)
  • For example, are these markers expressed on normal stem cells and are they conserved in the mammary gland in mice and other species? (aacrjournals.org)
  • assay
  • The functional ability per HSC, as well as the HSC concentration, was measured with minimal disruption to the cells in vivo using the new competitive dilution assay. (jax.org)
  • regulator
  • The p21 (CIP1/WAF1) protein binds to and inhibits the activity of cyclin - CDK2 , - CDK1 , and - CDK4 /6 complexes, and thus functions as a regulator of cell cycle progression at G 1 and S phase . (wikipedia.org)
  • yolk sac
  • Following the onset of circulation at about 21 days of development, yolk sac cells are found in embryonic blood. (bloodjournal.org)
  • The fetal liver subsequently replaces the yolk sac as the main hematopoietic organ 4 , 5 with appearance of definitive enucleate, macrocytic erythrocytes expressing fetal hemoglobins (eg, α 2 γ 2 globin chains). (bloodjournal.org)
  • respectively
  • They were either 10 or 4 HSC per million cells when tested in BALB or CByB6F1 recipients, respectively. (jax.org)
  • Given the limited material (approximately 1 million LSK + and LSK - cells, respectively, per experiment), we developed a procedure employing isobaric tags for relative quantification (iTRAQ). (bloodjournal.org)
  • physiology
  • We reasoned that the potential for OPN to affect stem cell function in the niche was great given its abundance in the proper geographic location, its receptor expression on stem cells, and the evidence for it affecting processes in other cells that might be relevant for stem cell physiology. (rupress.org)
  • molecular pathways
  • Components of stem cell niches have generally been defined in terms of cells and molecular pathways. (rupress.org)
  • The identification and characterization of tumor-initiating cells and the molecular pathways that account for their self-renewal and survival is critical to design therapies that preferentially target these cells and sensitize them to conventional chemotherapies and radiation therapies. (aacrjournals.org)
  • genes
  • Definition of cell form and function is derived from the transcription of specific sets of genes, followed by their translation and post-translational regulation. (bloodjournal.org)
  • 2 - 4 Alternatively, the critical stem cell characteristics can be defined by analysis of the function of specific genes (in the hematopoietic system, SCL, HOX-B4 , and BMI-1 5 - 7 ). (bloodjournal.org)
  • Differentially expressed genes in the Lin − CD29 H CD24 H mouse mammary gland tumor-initiating cell population include those involved in DNA damage response and repair, as well as genes involved in epigenetic regulation previously shown to be critical for stem cell self-renewal. (aacrjournals.org)
  • long-term
  • Fetal and young HSC in both types of recipients maintained clonal stability long term so that percentages of donor cells at 6 and 9 months were strongly correlated (r = 0.72 to 0.93, p (jax.org)
  • The nucleotide sugar UDP-glucose mobilizes long-term repopulating primitive hematopoietic cells. (nih.gov)
  • cord
  • Single-cell analysis identifies a CD33 + subset of human cord blood cells with high regenerative potential. (nih.gov)
  • system
  • Migration of mature and immature leukocytes in response to chemokines is not only essential during inflammation and host defense, but also during development of the hematopoietic system. (sigmaaldrich.com)
  • Thus we considered the hematopoietic system and found that seven days after poly injection, the number of blood cells in peripheral blood decreased drastically. (biomedcentral.com)
  • 1 Already in 1938 L. K. Diamond and K. D. Blackfan discussed the cause of this bewildering disease and proposed: "…there may be congenital insufficiency of red marrow tissue and inability on the part of the hematopoietic system to respond to the need for more blood as the erythrocytes wear out. (bloodjournal.org)
  • proliferating cell nu
  • p21 interacts with proliferating cell nuclear antigen ( PCNA ), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. (wikipedia.org)
  • Białko p21 (WAF1) jest w stanie oddziaływać z jądrowym antygenem komórek proliferujących ( proliferating cell nuclear antigen − PCNA), czynnikiem dodatkowym polimerazy DNA , i tym samym odgrywać rolę regulatora zarówno przy replikacji materiału genetycznego w fazie S jak i przy naprawie łańcucha . (wikipedia.org)
  • interactions
  • Interestingly, this latter effect was dependent on both cell-cell interactions and secreted factors. (biomedcentral.com)
  • OPN binds to cells through an arginine-glycine-aspartate-mediated interaction with integrins and non-arginine-glycine-aspartate-mediated interactions with CD44, activating multiple and varied signaling pathways. (rupress.org)
  • The key regulatory effects of major medullar HSPGs are described, focusing on their roles in the interactions between hematopoietic stem cells and their endosteal niche, and on their ability to interact with Heparin Binding Proteins (HBPs). (springer.com)
  • receptor
  • Publications] Nosaka K: 'Mechanism of hypercalcemia in adult T-cell leukemia : Overexpression of receptor activator of nuclear kB ligand on adult T-cell leukemia cells'Blood. (nii.ac.jp)
  • Tif1γ regulates the TGF-β1 receptor and promotes physiological aging of hematopoietic stem cells. (nih.gov)
  • Fiber mediated receptor masking in non-infected bystander cells restricts adenovirus cell killing effect but promotes adenovirus host co-existence. (lu.se)