Primitive and definitive hematopoietic stem cells embryonic cell differentiation. Medical search. Frequent questions

Dermal macrophages are either from embryonic progenitors or circulating progenitors. (wikipedia.org)
The prenatal population of dermal macrophages is gradually replaced by the recruitment and differentiation of circulating progenitors in adulthood. (wikipedia.org)
We found that MYB was not required for initial hematopoietic commitment of nascent mesoderm and emergence of primitive, yolk sac-type human hematopoietic progenitors. (nih.gov)
However, inactivation of MYB severely abrogated proliferation of the primitive erythroid and mixed erythroid-macrophage-megakaryocyte progenitors. (nih.gov)
Transposon-mediated rescue of MYB expression in MYB-null cells efficiently restored both the primitive hematopoietic progenitors and immature myeloid cells. (nih.gov)
This Notch ligand was also proven to regulate the hematopoietic destiny of individual hemato-endothelial progenitors (Ayllon et?al. (bioskinrevive.com)
endothelial progenitors, with CD43 manifestation marking the earliest step of hematopoietic commitment (Vodyanik et?al. (bioskinrevive.com)
2012). At later EB stage, most CD43+ cells upregulated the manifestation of CD41a and CD235a, and were enriched for megakaryocyte and erythroid progenitors, respectively (Klimchenko et?al. (bioskinrevive.com)
In vascular medicine, various stem cells and adult progenitors have been highlighted as having a vasoreparative role in ischaemic tissues. (biomedcentral.com)
Early in murine embryonic development, hematopoietic progenitors can be found in the para-aortic splanchnopleura region on embryonic day 7 (as depicted in Figure 1 A), but these progenitors are not LT reconstituting although they do possess lymphoid potential [ 11 - 13 ]. (biomedcentral.com)
These more advanced hematopoietic progenitors isolated from the aorta-gonad-mesonephros (AGM) region at embryonic day 9.5 to 10 can give rise to lymphoid lineages and are LT reconstituting definitive HSCs, but since this time point is after the establishment of the circulatory system in the embryo their source was difficult to determine [ 13 ]. (biomedcentral.com)
Cumano and colleagues' approach was to culture explants containing hematopoietic progenitors isolated from the yolk sac and the embryo proper before the onset of circulation, and to determine their LT reconstitution potential [ 10 ]. (biomedcentral.com)
Their results indicated that yolk sac progenitors before the onset of circulation did not have LT reconstitution potential while progenitors isolated from the AGM did, which indicates that primitive and definitive hematopoiesis may occur at distinct anatomical locations [ 10 ]. (biomedcentral.com)
Taken together, these fundamental studies provided the basis that the synovial macrophages were derived from hematopoietic progenitors and represented a heterogeneous population in both normal and inflamed synovium. (musculoskeletalkey.com)

To explore the role of MYB in human hematopoietic development we have inactivated the gene by bi-allelic TALEN-supported gene targeting in several lines of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), and subjected these cells to hematopoietic differentiation in well-defined cell culture conditions. (nih.gov)
The future direction of the study of hematopoietic stem cells will probably make use of pluripotent stem cells to yield specific immune cell lineages and eventual clinical applications. (biomedcentral.com)
Differentiation of human pluripotent stem cells (hPSCs) into functional cell types is a crucial step in cell therapy. (ashpublications.org)
In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)

Hematopoietic stem cells (HSCs) emerge from hemogenic endothelial (HE) cells, through endothelial-to-hematopoietic transition (EHT). (lu.se)
However, metabolic processes underlying the emergence of HSCs from HE cells remain unclear. (lu.se)
MYB is well recognized to be a key regulator of definitive hematopoiesis that plays an important role in the maintenance and multilineage differentiation of hematopoietic stem cells (HSCs). (nih.gov)
The second wave, referred to as definitive hematopoiesis, temporarily occurs in the fetal liver and transitions to the bone marrow and thymus, where multipotent hematopoietic stem cells (HSCs) contribute to the generation of all blood lineages ( 1 - 3 ). (amegroups.org)
Across vertebrate species, definitive HSCs are believed to be generated in the aorta-gonad-mesonephros (AGM) region. (amegroups.org)
These HSCs are primarily derived from endothelial cells within the ventral wall of the dorsal aorta. (amegroups.org)
The definitive HSCs in mammals first migrate to and repopulate in the fetal liver and then home to the bone marrow, while the definitive HSCs in zebrafish migrate first to the caudal hematopoietic tissue and then to their definitive anatomical sites in the kidney and thymus ( 5 - 8 ). (amegroups.org)
While this role has been best documented in the developmental migration of the neural crest and mesoderm, here we expand on previously reported preliminary findings that morpholino knock-down of snai2 impairs the generation of hematopoietic stem cells (HSCs) during zebrafish development. (bvsalud.org)

We have used RT-PCR to screen pluripotent murine embryonic stem cells to identify receptor tyrosine kinases (RTKs) potentially involved in the determination or differentiation of cell lineages during early mouse development. (silverchair.com)

The Hirschi Lab is broadly interested in endothelial cell differentiation, proliferation, specification and function during development. (hirschilabuva.com)
EPO supports the proliferation and differentiation of erythroid progenitor cells and is critical for their survival [ 1 ]. (biomedcentral.com)
We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application. (molvis.org)
The differentiation, proliferation, prognosis, and safety of MSCs for in vivo therapy remain controversial. (molvis.org)
Whereas it seems intuitive that an immune cell type that depends on regular proliferation outbursts for function may have evolved to modulate telomerase expression it is less obvious why others may also do so, as has been suggested for macrophages and neutrophils in some chronic inflammation disease settings. (biomedcentral.com)
B-cells have also been described to up-regulate telomerase expression during maturation, a process that also relies on proliferation bursts [ 16 ]. (biomedcentral.com)

Depletion of Runx1 with antisense morpholino oligonucleotides abrogated the development of both blood and vessels, as demonstrated by loss of circulation, incomplete development of vasculature and the accumulation of immature hematopoietic precursors. (biologists.com)
The administration of nonlethal irradiation to induce death of hematopoietic cells and their precursors followed by administration of donor bone marrow (radiation chimeras) revealed populations of macrophages, including synovial macrophages, Langerhans cells, and microglia, that were resistant to irradiation and remained host origin, whereas the monocyte population was derived from donor hematopoietic cells. (musculoskeletalkey.com)
Here, we have analyzed cis element regulation of the critical hematopoietic factor Gata2 , which is expressed in early precursors and repressed as GATA-1 levels rise during terminal differentiation. (prolekarniky.cz)

During embryonic development, hematopoiesis occurs through primitive and definitive waves, giving rise to distinct blood lineages. (lu.se)
Here, we show that the emergence of blood is regulated by multiple metabolic pathways that induce or modulate the differentiation toward specific hematopoietic lineages during human EHT. (lu.se)
Ligand-dependent development of the en-dothelial and hemopoietic lineages from embryonic mesodermal cells expressing vascular endothelial growth factor receptor 2. (chinagene.cn)

In zebrafish, primitive hematopoiesis occurs at early somite stages in the intermediate cell mass (ICM) and the rostral blood island (RBI). (amegroups.org)
Primitive hematopoiesis occurs at early somite stages in the ICM and the RBI. (amegroups.org)

The use of cell therapy for vascular regeneration offers an exciting new prospect in regenerative medicine. (biomedcentral.com)
However, the delivery of the correct cell type to the precise area of injury or vascular insufficiency is difficult and many factors need to be considered. (biomedcentral.com)
Cells for vascular therapy must be able to home to ischaemic or damaged tissue and engage in vessel formation alone or in unison with resident vasculature to achieve a controlled and functional reperfusion event, without causing pathological angiogenesis (for example, proliferative retinopathy in the vitreous of the eye). (biomedcentral.com)
While ER Clark first reported the observation of vascular generation in tadpoles in 1909, and Stockard described blood-vessel development and coined the term angioblast in fish embryos in 1915, it was not until 1920 that F Sabin described the formation of blood cells through the angioblast 'liquefying' within the aorta [ 1 - 3 ]. (biomedcentral.com)
VCAM-1, vascular cell adhesion molecule-1. (amegroups.org)
He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
Vessel development involves generating primary vascular endothelial tubes by aggregation of angioblasts (vasculogenesis), creating a vascular network through endothelial sprouting (angiogenesis), and pruning primary vascular tubes by recruiting smooth muscle cells to the vessel walls (vessel maturation). (chinagene.cn)
Vascular endothelial cell development and underlying mechanisms[J]. HEREDITAS, 2012, 34(9): 1114-1122. (chinagene.cn)
Notch signaling is required for arterial-venous differentiation during embryonic vascular development. (chinagene.cn)
Sonic hedgehog and vascular endothelial growth factor act up-stream of the notch pathway during arterial endothelial differentiation. (chinagene.cn)
Vascular perfusion is required for all tissues in the body to provide nutrients and oxygen, remove waste products, maintain fluid homeostasis, and traffic immune cells. (hirschilabuva.com)
Ongoing studies are further investigating underlying regulatory mechanisms, the role of endothelial cell cycle state in lymphatic specification, and the role of dysregulated of endothelial cell cycle state in vascular malformations and diseases. (hirschilabuva.com)
Vascular endothelial cell specification in health and disease. (hirschilabuva.com)

Figure 1 VCAM-1 + macrophages guide hematopoietic stem/progenitor cell to sites of definitive hematopoiesis. (amegroups.org)
The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)

The evaluation of the most suitable timing of cell delivery as well as the number of cells needed to integrate into resident vasculature and promote revascularisation of specific tissues requires careful optimisation and evaluation. (biomedcentral.com)
Cells Tissues Organs (2011) 193 (4): 239-252. (karger.com)
HSPC arterial amplification occurs prior to seeding of secondary hematopoietic tissues and proceeds, in part, through the cell cycle regulator myca (c-myc). (bvsalud.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The structural integrity of these tissues requires extensive cell-cell adhesion and interactions mediated by the adhesive junctional complexes consisting of the adherens junctions and desmosomes [ 2 - 5 ]. (oncotarget.com)
The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
In human disease studies, ethical concerns often restrict the collection of patient cells and tissues. (biomedcentral.com)
Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. (lookformedical.com)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

In both in vitro and in vivo settings, steering pyruvate use toward glycolysis or OXPHOS differentially skews the hematopoietic output of HE cells toward either an erythroid fate with primitive phenotype, or a definitive lymphoid fate, respectively. (lu.se)
We portrayed the Notch system in embryonic stem cell (ESC)-derived embryoid bodies (EBs) differentiating under the standard protocols used to assess yolk sac (YS) hematopoiesis in vitro. (karger.com)
Widespread Notch1 activation was evident at days 2-4 of EB differentiation, the time window of hemangioblast generation in this in vitro system. (karger.com)
Un rôle majeur des prostaglandines dans l'hématopoïèse, ainsi mis en évidence, a pu être étendu à la souris chez qui un traitement par la prostaglandine PGE2 provoque la multiplication des CSH in vivo et in vitro, un résultat qui génerera très probablement des applications cliniques. (hypotheses.org)
With the advent of new genomics technologies and improved biological in vitro differentiation systems, time series ChIP-seq data sets have been generated that allow for investigating chromatin states across multiple time points. (biomedcentral.com)
HADSCs were induced to confirm that they can express the characteristics of endothelial cells (ECs) in vitro. (molvis.org)
Therefore, in this study, we assessed the ability of human adipose mesenchymal stem cells (hADSCs) to express the characteristics of ECs after induction in vitro to evaluate the theoretical feasibility of maintaining or restoring the function of ECs after intraocular application. (molvis.org)
However, it also affects embryos created by in vitro fertilisation, which are manipulated or even disposed off when techniques such as pre-implantation genetic diagnosis - PGD are used to select healthy embryos and their subsequent gestation, to select children in parents with hereditary or genetic diseases, or to create embryos and later children in order to use their haematopoietic material to treat a sibling with a hereditary or genetic condition. (bioethicsobservatory.org)
Cells grown in vitro from neoplastic tissue. (lookformedical.com)

We demonstrate that glycolysis-mediated differentiation of HE toward primitive erythroid hematopoiesis is dependent on the epigenetic regulator LSD1. (lu.se)

Our data set up that hemogenic endothelium populations endowed with primitive and definitive hematopoietic potential are specified simultaneously from your mesoderm in differentiating hESCs. (bioskinrevive.com)
To time, it really is still as yet not known whether HE subsets with different hematopoietic potentials emerge in successive waves during hESC differentiation, whether HE populations are preserved inside the differentiating lifestyle as time passes, or whether one exclusive population of He's produced early from mesoderm and steadily differentiates inside the lifestyle. (bioskinrevive.com)
Following hemogenic potential of endothelium cell populations frequently during the period of hESC differentiation would address a few of these problems but to time this has hardly ever been reported. (bioskinrevive.com)
2006). In those ethnicities, different hematopoietic populations emerged from CD144+CD31+CD73? (bioskinrevive.com)
The first wave, defined as primitive hematopoiesis, takes place in the extraembryonic yolk sac and generates transitory hematopoietic cell populations consisting of primitive erythrocytes. (amegroups.org)
At least two populations of tissue macrophages exist: tissue-resident cells that are embryonically derived and a monocyte-derived population. (musculoskeletalkey.com)
A classification of B-lymphocytes based on structurally or functionally different populations of cells. (lookformedical.com)

In the vertebrate developmental context, MYB is widely regarded dispensable for primitive hematopoiesis but critically required for the development of definitive hematopoiesis. (nih.gov)
The hematopoietic developmental process has been studied in detail since the early 1900s. (biomedcentral.com)
(B) Subset of cell surface markers used in the characterization of this developmental process. (biomedcentral.com)
However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)
We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)
Research in the Hirschi Lab aims to uncover the developmental mechanisms of hemogenic endothelial cell specification and endothelial-to-hematopoietic transition, and apply insights gained to the generation of human HSPCs for clinical therapies. (hirschilabuva.com)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)

derivation of this specialized endothelium Ezetimibe enzyme inhibitor from human being embryonic stem cells (hESCs) provides an priceless platform to study and dissect blood specification and the emergence of hematopoietic stem and progenitor cells. (bioskinrevive.com)
In the last decade, there has been an increased desire for the characterization of this precursor from differentiating hESCs using several methods, generally through three-dimensional embryoid body (EB) differentiation (Ditadi et?al. (bioskinrevive.com)
2014). Using OP9 stromal cells to differentiate hESCs, both Rafii et?al. (bioskinrevive.com)
Human embryonic stem cells (hESCs) derived from an early embryo can self-renew indefinitely and differentiate into a variety of cell types. (ashpublications.org)

and the RBI cells are derived from the lateral mesoderm of the zebrafish head and differentiate into macrophages ( 4 - 8 ). (amegroups.org)
Here, we profile the transcriptome of the earliest detectable endothelial cells (ECs) during zebrafish embryogenesis to demonstrate that tissue-specific EC programs initiate much earlier than previously appreciated, by the end of gastrulation. (bvsalud.org)
Zebrafish runx1 is expressed in hematopoietic and neuronal cells during early embryogenesis. (biologists.com)
runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl , and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche . (biologists.com)
The block in definitive hematopoiesis is similar to that observed in Runx1 knockout mice, implying that zebrafish Runx1 has a function equivalent to that in mammals. (biologists.com)
Our data suggest that zebrafish Runx1 functions in both blood and vessel development at the hemangioblast level, and contributes to both primitive and definitive hematopoiesis. (biologists.com)
Moreover, we show that a subset of gut mpeg1.1 + cells express telomerase ( tert ) in young WT zebrafish, but that the relative proportion of these cells decreases with ageing. (biomedcentral.com)

Moreover, the level of MYB expression was highest at the commitment stage of differentiation and significantly decreased at the maturations stage. (nih.gov)
The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)

Snail2 is a zinc-finger transcription factor best known to repress expression of genes encoding cell adherence proteins to facilitate induction of the epithelial-to-mesenchymal transition. (bvsalud.org)
This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
Laminin 411 markedly induced the expression of the genes Foxa2 and Sox17 , markers for pancreatic precursor cells, efficiently induced IPC differentiation from MSCs, and up-regulated insulin expression at both mRNA and protein levels. (biomedcentral.com)
The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1). (lookformedical.com)

Primitive haematopoiesis allows the generation of yolk-sac derived macrophages and subsequent release into the foetal bloodstream for tissue infiltration and colonisation. (wikipedia.org)
In this process, cytokines like CSF1 are essential in facilitating the differentiation of monocytes into tissue-resident dermal macrophages and their survival. (wikipedia.org)
If the underlying ischaemia is not sufficiently resolved it can lead to tissue damage, with subsequent cell death. (biomedcentral.com)
This review will examine the clinical potential of several stem and progenitor cells that may be utilised to regenerate defunct or damaged vasculature and restore blood flow to the ischaemic tissue. (biomedcentral.com)
This leads to hypoxia and tissue damage as a consequence of the build up of waste metabolites and may result in cell death [ 1 ]. (biomedcentral.com)
A cell therapy approach should be aimed at promoting revascularisation of ischaemic tissue. (biomedcentral.com)
There is a therapeutic window in which to deliver the cells, to avoid extensive tissue damage, fibrosis and necrosis. (biomedcentral.com)
The lab also uses human stem cell culture models to study the regulation of human endothelial cell differentiation and specification, and to study the functional roles of endothelial cells in regulating tissue-resident stem cells. (hirschilabuva.com)
The tissue protective functions of the hematopoietic growth factor erythropoietin (EPO) are independent of its action on erythropoiesis. (biomedcentral.com)
The levels of catalase (CAT) and glutathione (GSH) were measured in PC12 cells and Drosophila brain tissue. (sdbonline.org)
GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
Notably, mpeg1.1 + macrophages have much longer telomeres in the gut than in their haematopoietic tissue of origin, suggesting that there is modulation of telomerase in these cells, in the gut. (biomedcentral.com)
Telomerase expression is also a hallmark of tissue stem cells, but its expression in these cells is still insufficient to completely prevent telomere shortening over time [ 11 ]. (biomedcentral.com)
Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. (lookformedical.com)

What started as a series of microscopic observations in different animal model systems has since evolved into studies of gene expression and regulation, and subsequent protein-protein interactions, cell surface protein expression profiling, and functional mapping of cell fates. (biomedcentral.com)
There is growing evidence that during primitive hematopoiesis in the developing embryo that macrophages generated in the yolk sac are involved in the regulation of primitive HSC trafficking-a mechanism suggested to involve in the secretion of inflammatory factors, extracellular matrix (ECM) components, and metalloproteinases ( 3 , 9 , 10 ). (amegroups.org)
Plakoglobin is a component of both the adherens junctions and desmosomes, and therefore plays a vital role in the regulation of cell-cell adhesion. (oncotarget.com)
Regulation of endothelial cell differentiation and specification. (hirschilabuva.com)
We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
however, little is known about a role of laminin 411 in the regulation of IPC differentiation from human MSCs. (biomedcentral.com)

2014). Definitive hematopoiesis, defined by T lymphoid potential, was restricted to the CD43? (bioskinrevive.com)
This development is also often referred to as fetal hematopoiesis, and was demonstrated by the production of embryonic erythrocytes that still possess a nucleus, express fetal hemoglobin, and lack any lymphoid potential [ 5 ]. (biomedcentral.com)
Overall, they demonstrated that the definitive HSC differs from the primitive HSC by lymphoid potential as well as in their ability to achieve LT reconstitution following transfer into adult recipients. (biomedcentral.com)
A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes. (lookformedical.com)
Lymphoid cells concerned with humoral immunity. (lookformedical.com)
Malignant lymphoma composed of large B lymphoid cells whose nuclear size can exceed normal macrophage nuclei, or more than twice the size of a normal lymphocyte. (lookformedical.com)
B-cell lymphoid tumors that occur in association with AIDS. (lookformedical.com)

Definitive haematopoiesis occurs from 11 days and onwards after fertilisation. (wikipedia.org)
Mouse hematopoiesis was thought to develop initially in the yolk sac, where primitive hematopoietic development occurs [ 4 ]. (biomedcentral.com)
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). (lookformedical.com)

B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. (lookformedical.com)

During this first wave, small number of myeloid cells (e.g., primitive monocytes/macrophages and megakaryocytes) are also generated. (amegroups.org)

Numerous dermal macrophages are present in the skin at birth due to the infiltration of yolk-sac derived macrophages and haematopoietic stem cell (HSC)-derived monocytes. (wikipedia.org)

Distinct anatomical regions of primitive and definitive hematopoietic development in both the mouse and human. (biomedcentral.com)
Chromatin states have initially been annotated in a spatial manner genome-wide, by segmenting the genome into distinct states based on histone modification ChIP-seq data from, for instance, one cell line, which represents an unsupervised learning problem. (biomedcentral.com)
In this way, investigating human immune-related diseases using iPS cells offers distinct advantages over conventional research methods. (biomedcentral.com)

There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
He performed some of the first studies to identify transcription factors that regulate the gene expression and differentiation of eosinophils, which are known to play a major role in allergic and asthmatic responses. (ubc.ca)
His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
dGLYAT was found to regulate Gadd45 -mediated JNK pathway activation and cell invasion. (sdbonline.org)

In addition, MYB-negative hESC/iPSC lines demonstrated major defects in myeloid cell development and completely failed to generate mature granulocytes. (nih.gov)
Notch receptors and Notch ligands were detected in virtually all cells throughout EB development. (karger.com)
In this review, we will discuss the milestone discoveries that have been achieved in the field of hematopoietic development, as well as the techniques that have been employed. (biomedcentral.com)
This initial wave of fetal hematopoietic development appears to have short-term reconstituting function, as demonstrated by Dieterlen-Lievre using chicken-quail chimeras [ 6 ]. (biomedcentral.com)
Primitive and definitive hematopoietic development. (biomedcentral.com)
The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
Embryonic development is tightly controlled at the level of gene expression. (amegroups.org)
Development of the dorsal aorta is a key step in the establishment of the adult blood-forming system, since hematopoietic stem and progenitor cells (HSPCs) arise from ventral aortic endothelium in all vertebrate animals studied. (bvsalud.org)
Hematopoietic stem cells are formed during embryonic development, and serve as the foundation of the definitive blood program for life. (bvsalud.org)
Although several factors such as vegf, flk1, cloche, lycat and estrp are essential for angioblast development, much of the signaling pathways underlying the derivation of angioblasts from the hemangioblasts or Flk1+ mesodermal cells remain unknown. (chinagene.cn)
Two molecules related to the VEGF receptor are expressed in early endothelial cells during avian embryonic development. (chinagene.cn)
Endothelial Cell Development and Its Application to Regenerative Medicine. (hirschilabuva.com)
Initial studies suggested that, in adults, a common monocyte/dendritic cell (MDP) progenitor in the bone marrow leads to the development of monocytes, macrophages, and dendritic cells. (musculoskeletalkey.com)
EPO and its receptors (EPOR) are expressed in multiple brain cells during brain development and upregulated in the adult brain after injury. (biomedcentral.com)
Telomerase expression is required for the maintenance of germ cells and during embryonic development, thereby ensuring the replicative potential over generations [ 10 ]. (biomedcentral.com)
In development, lineage-restricted transcription factors simultaneously promote differentiation while repressing alternative fates. (prolekarniky.cz)
GATA-2 has a broad role in hematopoietic development, as demonstrated by impaired hematopoiesis in Gata2 knock-out mice resulting in lethality during midgestation [8] , [9] . (prolekarniky.cz)

Monocytes are produced from haematopoietic stem cells in the liver, and they subsequently infiltrate the skin via the foetal bloodstream. (wikipedia.org)
Therefore, the differentiation of monocytes to dermal macrophages is CSF-1/CSF1R dependent. (wikipedia.org)

This complex process is regulated by the interplay of transcription factors with a chromatin environment, both of which provide the epigenetic information maintaining cell-type specific gene expression patterns. (amegroups.org)

However, the precise macrophage-specific signals regulating HSC recruitment to definitive sites of hematopoiesis are not well defined. (amegroups.org)

The gene reporter system showed that MYB is specifically expressed during hematopoietic commitment in the earliest primitive blood cells. (nih.gov)

Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
urgent intervention indicated Definition:A dysfunction characterised by insufficiently wholesome hematapoietic cell manufacturing by the bone marrow. (ehd.org)

2006). Using EB differentiation protocols, the onset of hematopoietic commitment was also defined from the manifestation of CD43, growing from a CD34+ endothelial precursor human population (Kennedy et?al. (bioskinrevive.com)
As immune cells typically undergo differentiation from precursor or naïve cells to mature cells, it is possible to observe the cell differentiation process by using iPS cell-derived immune cells. (biomedcentral.com)

This review will summarize our current knowledge, challenge, and future directions on molecular and cellular mechanisms of endothelial cell develop-ment. (chinagene.cn)
Ongoing research is focused on understanding mechanisms of endothelial cell specification towards hemogenic, arterial, venous and lymphatic fates. (hirschilabuva.com)
Endothelial cell specialization is associated with suppression of cell growth, but how signaling pathways that control endothelial cell cycle and fate are coordinated was not known. (hirschilabuva.com)
In subsequent studies using the Fluorescent Ubiquitination Cell Cycle Indicator (FUCCI) reporter, we found that endothelial cell cycle state determines propensity for arterial-venous fate. (hirschilabuva.com)
Endothelial Cell Cycle State Determines Propensity for Arterial-Venous Fate. (hirschilabuva.com)
Retinoic Acid Promotes Endothelial Cell Cycle Early G1 State to Enable Human Hemogenic Endothelial Cell Specification. (hirschilabuva.com)
Shear-induced Notch-Cx37-p27 axis arrests endothelial cell cycle to enable arterial specification. (hirschilabuva.com)

The cells were harvested at different time points for cell-sorting based on the expression of MYB-YFP and CD34 cell surface marker on day 6 and day 12. (nih.gov)
A higher proportion of CD34 + cells (∼ 20%) could be derived from hPSCs by inhibition of mitogen-activated protein kinase (MAPK) extracellular signal-regulated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling and activation of bone morphogenic protein-4 (BMP4) signaling. (ashpublications.org)
hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)
Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)

In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
Cadherins are single-pass transmembrane glycoproteins that form homotypic interactions with cadherin proteins on neighboring cells and interact intracellularly with proteins of the catenin family [ 4 , 5 ]. (oncotarget.com)
Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)

Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)
In addition to these basic issues concerning leukemia pathogenesis, we are devising new diagnostic procedures for detecting and monitoring leukemia patients based on molecular genetic abnormalities in the malignant cells. (stanford.edu)

In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)
Although Gata2 is normally repressed in late-stage erythroblasts, the −1.8 kb mutation unexpectedly resulted in reactivated Gata2 transcription, blocked differentiation, and an aberrant lineage-specific gene expression pattern. (prolekarniky.cz)

Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite-derived endothelial cells (SDECs) that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. (bvsalud.org)

Our results demonstrate that laminin 411 acts as a potent differentiation inducer of IPCs from UC-MSCs via the Pdx1 and Ngn3 signaling pathways. (biomedcentral.com)

RUNX1/AML1/CBFA2 is essential for definitive hematopoiesis, and chromosomal translocations affecting RUNX1 are frequently involved in human leukemias. (biologists.com)
Transient expression of runx1 in cloche embryos resulted in partial rescue of the hematopoietic defect. (biologists.com)

There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)

The first position is that of those who consider that the human embryo, in its first days of life, is a cell cluster with no biological structure, i.e. an unorganised cluster of cells and, accordingly, with no biological or ontological value. (bioethicsobservatory.org)
In other words, this law accepts the obsolete theory that identifies the human embryo as a cluster of cells. (bioethicsobservatory.org)
The third position is that of those who consider that the single-cell, polarised, asymmetrical human embryo, the zygote, obtained naturally or artificially, is a living being of our species, bearer therefore of the dignity that all human beings intrinsically possess, and consequently worthy of being treated in accordance with that dignity. (bioethicsobservatory.org)

T and B-cells cells are somatic cells that can break this rule and can modulate telomerase expression in a homeostatic manner. (biomedcentral.com)

2013). The performance of hematopoietic differentiation differs between your two methodologies because of parameters such as for example serum, stromal maintenance, or EB size, amongst others elements (Kardel and Eaves, 2012, Vodyanik et?al. (bioskinrevive.com)

Dermal macrophages are native immune cells mainly located in the dermis of the skin. (wikipedia.org)
They are also a type of antigen-presenting cells (APCs) that can mediate the infiltration of immune cells during an immune response, suggesting dermal macrophages' influence on both the innate and adaptive immune systems. (wikipedia.org)
Prenatal dermal macrophages are not to be confused with Langerhans cells as they uniquely express CD14 and FXIIIa and lack CD1a. (wikipedia.org)
The generation of dermal macrophages results from primitive haematopoiesis or definitive haematopoiesis. (wikipedia.org)
The generation of dermal macrophages is highlighted by the expression of cell markers such as CD64, MERTK and the downregulation of CCR2. (wikipedia.org)
Dermal macrophages can phagocytose and digest foreign substances similar to other cell types in the mononuclear phagocyte system. (wikipedia.org)
Dermal macrophages, Langerhans cells and dendritic cells are the main types of antigen-presenting cells (APCs) in the skin. (wikipedia.org)
Subset of macrophages expressing ICAM-1 migrate to the sites of definitive hematopoiesis in AGM and CHT to recruit hematopoietic stem cells and initiate neonatal hematopoiesis and guide them further to sites of adult and definitive hematopoiesis in kidneys and thymus. (amegroups.org)
It is now known that type A cells were synovial macrophages, type B were synovial fibroblasts, and type C were an undetermined population. (musculoskeletalkey.com)
Further studies in the 1980s and early 1990s refined the classification of synovial macrophages with the use of immunohistochemistry and known antibodies to antigen-presenting cells. (musculoskeletalkey.com)
Although CD4 T cells are the main targets of HIV infection, macrophages also become infected and resist the cytopathic effects of infection, contributing potentially to HIV reservoir persistence. (nih.gov)
In particular, iPS cell-derived monocytic cells and macrophages can be used for functional experiments, such as inflammatory cytokine production, and are often employed in research on patients with autoinflammatory diseases. (biomedcentral.com)
Specific absence of homeostatic, monocyte-independent macrophages altered the immune cell crosstalk in response to injury and induced proinflammatory neutrophil polarization, resulting in impaired cardiac remodelling without influencing infarct size. (elifesciences.org)
Using state-of-the-art fate-mapping models and genetic and pharmacological targeting approaches, the authors provide solid evidence that the absence of resident macrophages do not influence infarct size but instead alter the immune cell crosstalk in response to injury. (elifesciences.org)

Our findings reveal that during EHT, metabolism is a major regulator of primitive versus definitive hematopoietic differentiation. (lu.se)
MYB is an essential regulator of primitive human hematopoiesis in human pluripotent stem cell differentiation cultures. (nih.gov)

Further studies in mice by Cumano and colleagues sought to address the issue of an anatomical origin of the long-term (LT) reconstituting hematopoietic stem cell (HSC) found in adult animals [ 10 ]. (biomedcentral.com)

2012) demonstrated that insufficient Compact disc73 appearance proclaimed endothelium with hemogenic potential, as the upregulation of Compact disc73 marked dedication to endothelium without hematopoietic potential. (bioskinrevive.com)
Although SDECs lack hematopoietic potential, they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. (bvsalud.org)

Basic research utilizing immune-related disease patient-derived induced pluripotent stem (iPS) cells is expected to be a promising platform for elucidating the pathogenesis of the diseases and for drug discovery. (biomedcentral.com)
In summary, patient-derived iPS cells can provide a promising platform for pathological research and new drug discovery in the field of immune-related diseases. (biomedcentral.com)
Investigating human immune-related diseases using induced pluripotent stem (iPS) cells presents a novel and promising field of study. (biomedcentral.com)
We suggest an advantage of utilizing iPS cells for studying human immune-related diseases. (biomedcentral.com)
In this review, we discuss the application of iPS cells in the study of human immune-related diseases, with an emphasis on autoimmune diseases (Fig. 1 ). (biomedcentral.com)
A schematic summary of the investigation of immune-related diseases using patient-derived iPS cells. (biomedcentral.com)
Immune cells are somatic cells that break the rule, as they are capable of modulating telomerase expression in a homeostatic manner. (biomedcentral.com)
It has long been known that telomerase is up-regulated in adaptive immune cells, namely in T and B-cells [ 12 ]. (biomedcentral.com)

Particularly, non-hematopoietic cells, such as fibroblasts and cardiomyocytes, differentiated from autoimmune patient-derived iPS cells have shown promising results for further research into the pathogenesis. (biomedcentral.com)
In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

They can acquire an M1 or M2 phenotype to promote or suppress an inflammatory response, thereby influencing other cells' activity via the production of pro-inflammatory or anti-inflammatory cytokines. (wikipedia.org)

Early embryonic hematopoiesis in vertebrates proceeds in two consecutive waves ( Figure 1 ). (amegroups.org)

Recent studies, however, suggest that MDPs may also develop into other types of hematopoietic cells, such as lymphocytes. (musculoskeletalkey.com)
Another reason is that protocols for differentiating some lymphocytes associated with autoimmunity, such as CD4 + T cells or B cells, from iPS cells have not been well established. (biomedcentral.com)
They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. (lookformedical.com)
Most of these lymphomas represent the malignant counterpart of B-lymphocytes at midstage in the process of differentiation. (lookformedical.com)
A form of non-Hodgkin lymphoma having a usually diffuse pattern with both small and medium lymphocytes and small cleaved cells. (lookformedical.com)
A group of lymphomas exhibiting clonal expansion of malignant T-lymphocytes arrested at varying stages of differentiation as well as malignant infiltration of the skin. (lookformedical.com)

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. (thermofisher.com)
Insulin-producing cells (IPCs) differentiated from mesenchymal stem cells (MSCs) hold great promise in the treatment of DM. (biomedcentral.com)
Mesenchymal stem cells (MSCs) have gained interest because of its potential application in regeneration medicine and cytotherapy. (biomedcentral.com)

These results demonstrate that the cells that form neurospheres in culture are not stem cells at all, and that real stem cells are not able to form colonies under existing culture conditions. (elifesciences.org)

However, their antigen-presenting ability is relatively lower than dendritic cells (DCs) and Langerhans cells (LCs) in the skin. (wikipedia.org)
Recently, our groups established a method for differentiating dendritic cells that produce interferon-alpha, which can be applied as an SLE pathological model. (biomedcentral.com)

A region of the brain called the subventricular zone contains both neural stem cells and neural progenitor cells, and is one of only two regions of the brain where neural stem cells are found in adult mammals. (elifesciences.org)

In Drosophila with depleted RACK1 in all muscle cells or, specifically, in SC lineage resulted in a delayed recovery of skeletal muscle after physical damage as well as the low presence of active SC in the wound area. (sdbonline.org)

UC-MSCs were then cultured in the IPC induction and differentiation medium in the presence of laminin 411. (biomedcentral.com)

To date, a great deal of data describing the emergence of blood cells from human being HE have been acquired using stromal co-culture protocols Ezetimibe enzyme inhibitor (Choi et?al. (bioskinrevive.com)

Anatomy49

Organisms15

Diseases8

Chemicals and Drugs37

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes39

Disciplines and Occupations2

Information Science3

Named Groups1

Health Care1

Progenitors14

Pluripotent stem4

HSCs8

Murine embryonic1

Proliferation6

Precursors3

Lineages3

Hematopoiesis occurs2

Vascular13

Progenitor cell3

Tissues10

Vitro9

Erythroid1

Populations7

Developmental7

HESCs4

Zebrafish7

Stage of differentiation2

Genes4

Tissue14

Regulation7

Lymphoid7

Occurs3

Immature1

Myeloid cells1

Yolk1

Distinct3

Regulate4

Development18

Monocytes2

Chromatin1

Macrophage1

Earliest1

Bone marrow10

Precursor2

Endothelial cell7

CD345

Proteins3

Molecular2

Gene expression2

Dorsal aorta1

Pathways1

RUNX12

Transcriptional2

Embryo3

Modulate1

Differs1

Macrophages14

Regulator2

Anatomical1

Hemogenic2

Immune8

Fibroblasts2

Cytokines1

Waves1

Lymphocytes6

Mesenchymal3

Demonstrate1

Dendritic cells2

Found in adult1

Lineage1

MSCs1

Emergence1