• Detection of loop-mediated isothermal amplification (LAMP) end product by color changes. (ajtmh.org)
  • The objective of this study is to develop a visual reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of hMPV and applied to the clinical samples. (biomedcentral.com)
  • Oligonucleotide primer sequences derived from Rickettsia conorii genes (accession no. (cdc.gov)
  • Primer sequences derived from ( 9 ). (cdc.gov)
  • Primers were designed to target sequences in the nodavirus capsid protein gene, yielding an amplification product of 120 nucleotides. (int-res.com)
  • A cohort of 80 healthy subjects of Western European descent was screened to evaluate and validate the detection of exomic sequences of the coding genes with 25 base pair exon padding. (researchgate.net)
  • Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)
  • The nucleotide sequences of the glycoprotein L gene served as a target for amplification. (crie.ru)
  • The sequences of reference strains published in GenBank were used to analyze and design the primers. (crie.ru)
  • RT-LAMP employs a set of six specially designed primers that recognize eight distinct sequences of the target, and only when three pairs of specific primers match the target sequence exactly can the gene amplification start. (biomedcentral.com)
  • one includes structural genes encoding enzymes that directly participate in the formation of anthocyanins and other flavonoids, and the other comprises regulatory genes controlling the transcription of structural genes. (scirp.org)
  • The S gene is one of the structural genes of the virus which encodes for a protein that sits on the surface of the SARS CoV-2 virus. (who.int)
  • Total RNA was isolated on P16, and the relative abundance of mRNA of the crystallin structural genes, redox components, and apoptotic cascade were ascertained with real-time PCR with reference to the internal control β-actin . (molvis.org)
  • Amplification and detection were performed at 41°C for 90 min in a Corbett Research Rotorgene. (int-res.com)
  • Based on the detection of cell culture-derived nodavirus, and a synthetic RNA target, the real-time NASBA procedure was approximately 100-fold more sensitive than single-tube RT-PCR. (int-res.com)
  • The innovative system combines liquid-based sample preparation with downstream PCR detection of your region of interest using CRISPR-Q Custom PCR Assays and optional sequencing using CRISPR-Q Sanger Primers. (qiagen.com)
  • After amplification the samples are typed by hybridization to the typing strips followed by colorimetric detection. (cdc.gov)
  • The conserved regions of ORF6 and ORF7 are often used as target regions for PRRSV detection by nucleotide-based assays (18-22). (researchsquare.com)
  • PCR cycling conditions were: 2 min at 50˚C, then 10 min at 95˚C followed by 40 cycles each consisting of 15 sec at 95˚C and 1 min at 60˚C. The PCR plates were covered with optical adhesive sheets and placed for amplification in 7500 Fast Real-Time PCR System (Applied Biosystems) using the Accu SEQ Real-Time PCR Detection Software v2.1. (scirp.org)
  • CoV-2 genome and can be targeted as a region for detection of VOC/VOI. (who.int)
  • PathoSEEK Detection Assays are multiplex tests based on gene amplification and detection by quantitative PCR. (medicinalgenomics.com)
  • Each PathoSEEK Detection Assay contains primers and probes specific for the target species as well as an internal cannabis control. (medicinalgenomics.com)
  • en] The detection of foot-and-mouth disease (FMD) virus from various kinds of field samples (tissue extract and cell culture isolate) was studied using the polymerase chain reaction (PCR) technique. (iaea.org)
  • This device is a real-time PCR test for the qualitative detection of exon 19 deletions and exon 21 (L858R) substitution mutations of the epidermal growth factor receptor (EGFR) gene in DNA derived from formalin-fixed paraffin-embedded (FFPET) human non-small cell lung cancer (NSCLC) tumor tissue. (pdffox.com)
  • For manual sample preparation, FFPET specimens are processed using the cobas® DNA Sample Preparation Kit and the cobas z 480 analyzer is used for automated amplification and detection. (pdffox.com)
  • 2. The cobas® EGFR Mutation Test v2 kit provides reagents for automated real-time PCR amplification and detection of the EGFR mutations. (pdffox.com)
  • Noteworthy, the study of Vg gene would be helpful to understand the molecular mechanisms of development and would be used to establish a bio-monitoring tool for detection of exposure to different EDCs. (ac.ir)
  • As a result, the expression of genes directly involved in colistin biosynthesis and secretion increased, indicating that at the transcriptional level spo0A and abrB played opposite roles in regulating colistin biosynthesis in P. polymyxa . (hindawi.com)
  • Taken together, our data demonstrated that starch instead of glucose can promote colistin production probably by affecting the expression of colistin biosynthesis-related genes, as well as reducing the repression of glucose to a secondary metabolic product. (hindawi.com)
  • In order to enhance berry coloration of bright-red grape cultivars, the effects of abscisic acid (ABA) treatment on the quantity and composition of anthocyanins as well as the expression of genes related to flavonoid biosynthesis in the berry were examined. (scirp.org)
  • Quantitative real-time PCR analysis revealed that ABA treatment around véraison resulted in the upregulation of genes encoding enzymes responsible for both general flavonoid and anthocyanin biosynthesis. (scirp.org)
  • The Cell Lysis Buffer provides efficient room-temperature lysis without affecting downstream target amplification (see figures Cell Lysis Buffer rapidly lyses cells at room temperature. Lysis of 12,000 MCF7 (A) and U2OS (B) cells at RT, 37°C and 50°C for 5, 30, 60 and 90 min. The lysis reaction was inactivated at 80°C for 10 min. PCR was carried out using the CRISPR-Q Custom PCR Assay targeting ANTXR1. The presence of PCR product was verified using the QIAxcel Advanced. "> Cell Lysis Buffer rapidly lyses cells at room temperature and Target amplification unaffected by cell lysis buffer. Cell Lysis Buffer efficiently lyses cells without affecting downstream target amplification. Different volumes of a raw cell lysate (100 cells/µl) generated with the Cell Lysis Buffer were added to an AllTaq PCR. Target amplification of ELOVL6 (A) and EMX1 (B) was performed using CRISPR-Q Custom PCR Assays. "> Target amplification unaffected by cell lysis buffer ). (qiagen.com)
  • This test, using Hybrid Capture 2 technology, is a nucleic acid hybridization microplate assay with signal amplification. (cdc.gov)
  • This assay uses Roche Linear Array HPV Genotyping test that is based on HPV L1 consensus polymerase chain reaction (PCR) with biotinylated PGMY09/11 primer sets. (cdc.gov)
  • The results revealed that two of the real-time RT-PCR assays had high sensitivities and specificities, whereas the real-time RT-PCR assay of the OIE had low sensitivity due to mismatches between nucleotides of Korean PRRSVs and forward primers. (researchsquare.com)
  • A 10× Assay Mix of the primers and probe was prepared containing 10 µM of each primer and 2.5 µM probe. (scirp.org)
  • Each lot of HotStarTaq Plus DNA Polymerase is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified. (qiagen.com)
  • All PathoSEEK qPCR assay internal controls target a sequence in the cannabis genome, which helps to ensure plant cells have been lysed to release pathogens growing endophytically, DNA survived extraction intact, and there is no contamination in your Assay Master Mix. (medicinalgenomics.com)
  • In this study, we established a RT-LAMP assay to detect hMPV, and the amplification result can be observed by hydroxynaphthol blue (HNB) dye-mediated visualization using the naked eye. (biomedcentral.com)
  • To test this hypothesis, the phylogenetic composition of faecal microbiomes was investigated by a 16S rRNA gene amplicon sequence-based approach for Bacteria and Archaea . (springer.com)
  • Current evidence has been limited to mainly cross-sectional studies using 16S rRNA gene sequencing, attempting to answer the question 'who is there? (bmj.com)
  • Further, with advancing technologies, microbiome studies are shifting beyond the examination of the 16S rRNA gene, to include whole metagenome and metatranscriptome sequencing, as well as metabolome characterisation. (bmj.com)
  • 1981 ). Although more than 400 mutations have been reported in the COL4A5 gene, of which missense mutations account for 30%, there are no hotspot mutations. (researchsquare.com)
  • Some mutations in the S gene may lead to changes in the spike protein which result in inhibition of contact and entry of the virus into human cells, however in the case of the VOC, they contain mutations in the S gene that enhance the process of contact and entry into human cells, increasing transmissibility of the virus. (who.int)
  • Currently, manufacturers are focusing on and targeting assays to mutations in the S gene. (who.int)
  • Application of TILLING to search for mutants in 14 genes identified 21 to 46 mutations per gene, which correspond to a total of 450 mutations. (biomedcentral.com)
  • Missense mutations were found for all genes while truncations were selected for all except one. (biomedcentral.com)
  • In addition, the size of saturated populations containing insertion mutants randomly generated for most genes of an organism is extremely high because each line carries only a rather small number of mutations [ 6 ]. (biomedcentral.com)
  • Therefore, an allelic series of induced mutations with different effects on gene function can be easily isolated by screening a few thousands mutagenized plants [ 6 ]. (biomedcentral.com)
  • Bacteria can acquire resistance by gaining mobile antibiotic resistance genes or by de novo mutations in pre-existing DNA. (biomedcentral.com)
  • Missense mutations in the splicing factor gene PRPF31 cause a dominant form of retinitis pigmentosa (RP11) with reduced penetrance. (molvis.org)
  • For example, in the hereditary cancer syndromes familial adenomatous polyposis or hereditary nonpolyposis colorectal cancer, mutations have been discovered in the APC gene and DNA mismatch repair genes. (medscape.com)
  • ABSTRACT: Nucleic acid sequence based amplification (NASBA) is an isothermal nucleic acid amplification procedure based on target-specific primers and probes, and the co-ordinated activity of 3 enzymes: AMV reverse transcriptase, RNase H, and T7 RNA polymerase. (int-res.com)
  • All PathoSEEK primers and probes were tested in-silico against the cannabis genome to ensure no cross-reactivity. (medicinalgenomics.com)
  • Other platforms that use primers and probes borrowed from food testing may inadvertently hit the cannabis genome. (medicinalgenomics.com)
  • Primers and fluorescent probes were synthesized by the Eurogen (Russia). (biomicsj.ru)
  • These primers amplify approximately 600 basepairs of the ITS1-5.8S-ITS2 region of the ribosomal cistron. (cdc.gov)
  • These primers amplify approximately 620 basepairs of the 28S region of the ribosomal cistron. (cdc.gov)
  • These primers amplify approximately 717 bp of the coding region of the EF-1α gene. (cdc.gov)
  • These primers amplify approximately 495 bp of exons and introns at the 5' end of the β-tubulin gene. (cdc.gov)
  • These primers amplify a section of the intergenic spacer in the ribosomal cistron. (cdc.gov)
  • Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. (cdc.gov)
  • Following the cDNA synthesis, specifically - designed primers were employed to amplify the Vg gene and ultimately sequence it. (ac.ir)
  • Using the GenBank database ( www.ncbi.nlm.nih.gov ), we selected primers RcF (5′-GACGCTGCGTGAGTGATGA-3′) and RcR (5′-CCGGTGCTTCTTTACGCAGTA-3′), and probe RcS (5′-6 carboxyfluorescein-CTTTCGGGT T GTAAA A CTCTTTCGC G CA-Black Hole Quencher 1-3′), which amplify parts of the 16S rRNA encoding gene, to specifically amplify Rhabdochlamydia spp. (cdc.gov)
  • Amplification products were detected in real-time with a molecular beacon (FAM labelled/methyl-red quenched) that recognised an internal region of the target amplicon. (int-res.com)
  • CoralLoad Concentrate can be added to the PCR without affecting amplification sensitivity or specificity. (qiagen.com)
  • Therefore, LAMP and RT-LAMP method have been used as a powerful gene amplification tool due to its high specificity and sensitivity under isothermal condition. (biomedcentral.com)
  • HotStarTaq Plus Master Mix Kit outperforms kits from other suppliers and ensures high specificity and superior performance in hot-start PCR (see figures " Highest specificity. Highest specificity. "> Highest specificity " and " Higher specificity with different primer-template systems. Higher specificity with different primer-template systems. "> Higher specificity with different primer-template systems ", and table). (qiagen.com)
  • No cross-amplification was observed when the analytical specificity was tested with human, amebal ( Acanthamoeba castellanii ATCC 30010), and bacterial DNA ( Technical Appendix ). (cdc.gov)
  • In recent decades, it has been confirmed that tumor progression of GC is a multi-step process that involves the activation of oncogenes and the inactivation of tumor suppressor genes at different stages ( 7 , 8 ). (jcancer.org)
  • p21 and other tumor suppressor genes' inactivation are therefore most likely involved in the first steps of the cancer model, with later stages possibly involving protooncogene activation and inflammation. (medscape.com)
  • Regions of these genes were targeted for amplification in a single multiplex PCR reaction. (usda.gov)
  • Un test d'amplification en chaîne par polymérase (PCR) multiplex a également été mis au point pour identifier les isolats, et il s'est avéré que cette autre solution constituait un test diagnostique rapide, sensible et précis. (who.int)
  • This document describes some of the target genes and primers that can be used for DNA sequence-based identification of fungi and the PCR conditions with which to use those primers. (cdc.gov)
  • Advances in genetic diversity studies, genome mapping and advanced high-throughput sequencing technologies have helped identify many stress-responsive adaptive genes, quantitative trait loci (QTLs) and other useful crop traits in the CWRs. (bvsalud.org)
  • Although the ITS primers are universal for fungi, the D1D2 region of the large ribosomal subunit has better discrimination for yeasts, with primers NL-1 and NL-4. (cdc.gov)
  • Genes upregulated in the treated B. burgdorferi included a number of Erp genes and rplU , a 50S ribosomal protein. (frontiersin.org)
  • For identification of Fusarium isolates within species complexes, the EF-1α primers should be used (O'Donnell, 2009). (cdc.gov)
  • For identification of Scedosporium , Aspergillus , and Penicillium isolates within species complexes, the β-tubulin primers should be used (Glass, 1995). (cdc.gov)
  • Scientists at the Beltsville Agricultural Research Service, Beltsville, Maryland created mock communities from 5 common fungal isolates found in weanling piglet feces and assessed the potential biases in mycobiome identification based on DNA input, primer target choice, complexity of the community, and the databases utilized for analysis of Illumina MiSeq datasets. (usda.gov)
  • To better understand the impacts of these factors, we created mock communities from 5 common fungal isolates found in weanling piglet feces and assessed the potential biases in mycobiome identification based on DNA input, primer target choice, complexity of the community, and the databases utilized for analysis of Illumina MiSeq datasets. (usda.gov)
  • Most studies addressing travel-related changes in the faecal flora have focused on specific mobile resistance genes, or depended on culturing of individual bacterial isolates. (biomedcentral.com)
  • This identified approximately 140 chromosome loci where nucleotides deviated from the reference sequence GRCh37 hg19 comprising the three genes TRPV1, LTB4R and LTB4R2. (researchgate.net)
  • The locus of the psl polysacchide genes encode the cells to adhere to a surface and thus maintaining the biofilm structure. (ukessays.com)
  • In general, for unknown molds, the ITS region of the rDNA is used as the primary target with primers ITS-1 and ITS-4 as the most general primer set. (cdc.gov)
  • The method was first described by Avaniss-Aghajani et al in 1994 and later by Liu in 1997 which employed the amplification of the 16S rDNA target gene from the DNA of several isolated bacteria as well as environmental samples. (wikipedia.org)
  • Sample lysis and amplification is also tolerant of residual amounts of common reagents such as cell growth media, culture dish coatings and transfection/transduction reagents (see table below and figure Increased presence of medium might affect CRISPR target amplification. MCF7 or Jurkat cells (1000 cells/µL) were lysed according to the QIAprep&amp CRISPR Kit protocol. Before cell lysis, cells were either washed or remained unwashed. Additionally, culture medium was stepwise added to washed cells before lysis until 50% of the lysate was culture medium. All downstream steps were performed according to the QIAprep&amp CRISPR Kit protocol. "> Increased presence of medium might affect CRISPR target amplification ). (qiagen.com)
  • DNA amplification with polymerase chain reaction (PCR) is a ubiquitous protocol that scientists across academia and the industry know well. (benchling.com)
  • At this point, polymerase, the active enzyme, builds off of the primer to form the new DNA strand. (benchling.com)
  • Although next generation sequencing has rapidly improved our understanding of the microbiome, mycobiome studies necessitate careful assessment of techniques including DNA isolation method, primer design and choice, polymerase selection, sequencing platform, and reference database composition. (usda.gov)
  • The gene selected for diagnosis was the polymerase gene and an amplification target product of 454 bp in length was produced using AP5/AP6 primer sets. (iaea.org)
  • Furthermore the FMD RNA polymerase gene could be detected by the PCR method in samples negative in both ELISA typing and the virus isolation test. (iaea.org)
  • Our goal was to examine gene transcription by B. burgdorferi following doxycycline treatment in an effort to identify both persister-associated genes and possible targets for antimicrobial intervention. (frontiersin.org)
  • Chlamydia trachomatis is an obligate intracellular human pathogen that exhibits stage-specific gene transcription throughout a biphasic developmental cycle. (plos.org)
  • Representative σ 66 -dependent gene transcription was repressed in the absence of rsbV1 or upon increased expression of RsbW, and increased upon elevated expression of RsbV1. (plos.org)
  • Six primers were designed and synthesized with master mix reagents, and a phenol red indicator was applied to promote LAMP technique. (who.int)
  • All forward primers incorporated a 5' universal sequence complementary to a fluorescently-label complementary universal probe containing FAM. (usda.gov)
  • Another modification which is sometimes used is to fluorescently label the reverse primer as well using a different dye, again resulting in two parallel profiles per sample each resolving a different number of variants. (wikipedia.org)
  • In manual primer design, you can also set restriction enzyme cut sites, overhangs, and check secondary structure to see Gibbs Free Energy values for monomers or homodimers. (benchling.com)
  • The rpoB gene target sequence was amplified using mutagenesis forward and reverse primers followed by HindIII enzyme digestion. (who.int)
  • The forward and reverse primers, designed in-house, were 5'-gttcccgggccttgtacacac-3' and 5'-aagtggtaagcgccctcccg-3', respectively. (scirp.org)
  • The obtained results indicated that the designed primers successfully amplified the partial cDNA sequence. (ac.ir)
  • Our previous studies indicated that knockdown of the p65 gene or IKKβ inhibited lipopolysaccharide (LPS)-induced upregulation of TF and PAI-1 in rat AECII ( 15 , 16 ), indicating that the NF-κB signaling pathway participates in coagulation and fibrinolysis regulation in LPS-treated AECⅡ cells. (spandidos-publications.com)
  • In many cases, the disease gene encodes a component of phototransduction or allied processes with expression confined to the retina, for example the rod visual pigment (reviewed in [ 1 ]), or peripherin-RDS [ 2 ], but not all RP genes show such a restricted pattern of expression. (molvis.org)
  • The culture was done, and Enterococcus species were tested for antimicrobial susceptibility and carriage of v anA-D gene subtypes. (biomedcentral.com)
  • In some cases, these primers may not provide sufficient identification, and a protein coding region may be required. (cdc.gov)
  • The viral S gene is important as it codes for the Spike protein which is the molecule that makes contact with, and allows entry of the virus into susceptible host cells, causing infection. (who.int)
  • The completed rice genome sequence has been used for the characterization of a large number of gene families involved in diverse processes and pathways. (biomedcentral.com)
  • With the advances in genome sequencing, molecular breeding, gene expression analysis and multi-omics approaches, various attempts have been made to explore the genetic architecture of rice grain pigmentation. (bvsalud.org)
  • As a complementary resource, chemically induced mutants have been shown to provide an efficient alternative because each individual line can bear single point missense and nonsense substitutions in hundreds of genes [ 7 ]. (biomedcentral.com)
  • 80%) and biological pathways (such as adipocytokine, Notch, Hedgehog and NOD-like receptor signaling) were not identified by previous gene array studies. (mdpi.com)
  • Methods A next-generation sequencing (NGS) panel was created for the human TRPV1 gene and in addition, for the leukotriene receptors BLT1 and BLT2 recently described to modulate TRPV1 mediated sensitisation processes rendering the coding genes LTB4R and LTB4R2 important co-players in pharmacogenetic approaches involving TRPV1. (researchgate.net)
  • Therefore, characterization of Vg gene is of high priority for EDCs bio-monitoring. (ac.ir)
  • Samples were diluted 1:100 (v/v) in formamide containing CHIMERx GENEFLO 625 DNA Ladder and separated in an ABI 3100 Avant gene analyzer. (usda.gov)
  • Methods: Fifteen genes were identified based on their distribution's ability to discriminate different serotypes of S. enterica. (usda.gov)
  • To investigate the effects of loss of β1 function in vivo , we have used gene-targeting methods to produce β1(-/-) mice, and we have analyzed their neuronal phenotypes. (jneurosci.org)
  • Like most other community analysis methods, TRFLP is also based on PCR amplification of a target gene. (wikipedia.org)
  • In the right side window, you can assess your primers for GC content, melting temperature, and length. (benchling.com)
  • This study underscores the importance of experimental design and preliminary studies to assess primer combinations and database choice for the mycobiome sample of interest. (usda.gov)
  • C-PC treatment possibly prevented cataractogenesis triggered by sodium selenite, by regulating the lens crystallin, redox genes, and apoptotic cascade mRNA expression and thus maintains lens transparency. (molvis.org)
  • In addition, use of starch stimulated the expression of regulatory gene spo0A but repressed the expression of another regulatory gene abrB . (hindawi.com)
  • In particular, extracellular-regulated kinase (ERK) 1/2, one of the MAPK pathway is considered as an important mediator of signal transduction processes, namely cell survival, cell division, gene expression, and cell metabolism that plays role in injury, death, and inflammation of kidney tubular cells due to cisplatin administration [ 5 ]. (thieme-connect.com)
  • In agreement with GSS activity localization in vivo , we identified six genes encoding arylsulfatase-like enzymes with a predicted C-terminal transmembrane domain, of which five showed GSS activity upon heterologous expression in insect cells. (nature.com)
  • This study was designed to examine the constrictive potential of C-Phycocyanin (C-PC) in regulating changes imposed on gene expression in the selenite-induced cataract model. (molvis.org)
  • Treatment with 200 mg/kg bodyweight C-PC (Group III) transcriptionally regulated the instability of the expression of these genes, thus ensuring C-PC is a prospective anticataractogenic agent that probably delays the onset and progression of cataractogenesis induced by sodium selenite. (molvis.org)
  • The primary data for 1-step growth, 2-step growth and gene expression summarized in Figs 4 and 5 are available in the S1 Dataset in the supplementary information. (plos.org)
  • In this research project we try to quantify the gene expression of the above genes pilB, pslA, lecA, ureB using rpoD as the house keeping gene. (ukessays.com)
  • The gene expression is quantified using real time PCR. (ukessays.com)
  • The results from the planktonic and biofilm gene expression results are compared. (ukessays.com)
  • Recently, reduced expression of CDK10 has been observed in several cancerous human tissue, suggesting that CDK10 is a tumor suppressor gene. (jcancer.org)
  • An ERh gene expression profile was identified and was markedly different from the ERa signature. (lu.se)
  • Conclusion: Expression of ERh is an independent marker for favorable prognosis after adjuvant tamoxifen treatment in ERa-negative breast cancer patients and involves a gene expression program distinct from ERa. (lu.se)
  • The method is based on digesting a mixture of PCR amplified variants of a single gene using one or more restriction enzymes and detecting the size of each of the individual resulting terminal fragments using a DNA sequencer. (wikipedia.org)
  • On the other hand, the gene expressions of enzymes involved in proanthocyanidin synthesis were drastically decreased at véraison and remained extremely low even with ABA treatment. (scirp.org)
  • Both enzymes are tetramers (X 2 Y 2 ), where DNA gyrase encoded by genes gyrA and gyrB, and topoisomerase IV is encoded by parC and parE . (biomedcentral.com)
  • For the forward primer there are two options. (cdc.gov)
  • OF, outer forward primer. (cdc.gov)
  • From there, similar to the first method, you will click and drag your cursor on the sequence map to highlight the region where you wish to attach your forward primer. (benchling.com)
  • Click Set from Selection , and then select Forward for your forward primer. (benchling.com)
  • Such antibiotics are able to stop the growth of the bacteria such that the immune system can target and clear the infection. (frontiersin.org)
  • Conclusion Genetic testing prior to pregnancy is recommended in Alport syndrome patients or family members prior to pregnancy to determine the specific location of the gene mutation. (researchsquare.com)
  • Since then the method has been applied for the use of other marker genes such as the functional marker gene pmoA to analyze methanotrophic communities. (wikipedia.org)
  • The appropriate primer sets for each of the three endemic sero-types were optimized and used to detect the PCR products from field isolate viruses. (iaea.org)
  • Splicing of RHO intron 3 and GNAT1 introns 3-5 mini-gene templates was inefficient with both spliced and unspliced products clearly detected. (molvis.org)
  • The COL4A5, COL4A3, and COL4A4 genes have 53, 52, and 48 exons, respectively, and it is estimated that only 10% of all possible pathogenic COL4A5 variants have been reported (Gubler et al. (researchsquare.com)
  • Universal primer sets exist, but they often do not have enough discriminatory power to identify species, or they do not have the discriminatory power to identify species within a species complex, often giving 100% match to multiple species. (cdc.gov)
  • The ITS primer set generally only discriminates Fusarium species into the various species complexes but does not discriminate cryptic species. (cdc.gov)
  • Similar to Fusarium , the ITS primer set generally only discriminates Scedosporium , Apsergillus , and Penicillium species into the various species complexes but does not discriminate cryptic species. (cdc.gov)
  • Trichophyton species DNA is amplified very poorly by the ITS primer set used for most other molds. (cdc.gov)
  • Individual species of Trichosporon are not well discriminated using either the ITS or D1D2 primer sets. (cdc.gov)
  • Primer target region was a critical determinant of species output as well with biases seen based on gene copy number and primer design. (usda.gov)
  • Primer target region was a critical determinant of species output. (usda.gov)
  • Whole-exome data of the COL4A5 gene of the patient's family were analyzed using Sanger sequencing and online software was used to predict the conservation and pathogenicity of mutant amino acids within species evolution. (researchsquare.com)
  • Arabidopsis thaliana is the main model species for plant molecular genetics studies and world-wide efforts are devoted to identify the function of all its genes. (biomedcentral.com)
  • A major challenge in plant biology is the identification of biological functions for all genes from the main model plant species, Arabidopsis thaliana and rice. (biomedcentral.com)
  • A few gene therapy trials now target head and neck cancer, which comprises only 4% of all cancers but has a dismal prognosis in advanced stages. (medscape.com)
  • Six primers, including two outer primers (F3 and B3), two inner primers (FIP, BIP) and two loop primers (LF and LB), were designed for hMPV N gene by the online software. (biomedcentral.com)
  • Benchling will then retrieve any suitable primers from your selected folder and display them in the search results. (benchling.com)
  • It is suitable for large-scale sequencing of TRPV1 and functionally related genes. (researchgate.net)
  • The Benchling Life Sciences R&D Cloud includes tools to model a PCR product, and design and visualize primers for PCR. (benchling.com)
  • At the end of the extraction for ELISA the amplification product band at 454 bp was detected in 74% of the negative tissue extract samples, and in 48% at the end of the virus isolation procedure. (iaea.org)
  • Terminal restriction fragment length polymorphism (TRFLP or sometimes T-RFLP) is a molecular biology technique for profiling of microbial communities based on the position of a restriction site closest to a labelled end of an amplified gene. (wikipedia.org)
  • Your target sequence will show in the Molecular Biology application, which will offer a host of insights, including a linear map and any relevant metadata. (benchling.com)
  • There is a special set of ITS primers specifically for amplification of the ITS region of dermatophytes, especially Trichophyton (Gräser, 2000). (cdc.gov)
  • From there, left click and drag your cursor to highlight the region where you want your primer to bind. (benchling.com)
  • You can adjust the number of bases in your primer by clicking and dragging the black bars that border the highlighted region of your sequence. (benchling.com)
  • region 228440 to 229981) was the amplification target for quantification of hr DNA by PCR. (scirp.org)