Primers sequence analysis dna. Medical search. Frequent questions

Each mutation was verified by Sanger sequencing of PCR products spanning the variants ( Fig. 1 ). (nature.com)
However, traditional Sanger sequencing-based DNA barcoding protocols can be prohibitively expensive and laborious when large community samples have to be processed. (springer.com)
Below are instructions on how to register to iLab and create a service request for Sanger and fragment analysis services. (helsinki.fi)

RESEARCH element of the integrative conjugative element group lowed by Xba I digestion of genomic DNA. (cdc.gov)
The process starts from extracted genomic DNA, which contains both mtDNA and nuclear DNA, without the need to purify mitochondria. (northwestern.edu)
Total genomic DNA is the starting material for mtDNA-seq. (northwestern.edu)
Do I need specific DNA extraction kit or procedure to purify genomic DNA that contains mtDNA? (northwestern.edu)
Following the successful identification of genomic factors present in Lso haplotypes that are associated with ZC symptom development in potato, specific primers will be designed for use in targeted polymerase chain reaction (PCR) assays. (usda.gov)
DNMT1 preserves the methyltransferase by binding to hemi-methylated CpG sites and methylates the cytosine on the newly synthesized strand after DNA replication, whereas DNMT3a/DNMT3b are required for the de novo genomic methylation of DNA ( 15 ). (spandidos-publications.com)
Genomic DNA from both strains was analyzed by PCR using a set of 13 primer pairs that are specific for regions of the IncA/C plasmid backbone. (usda.gov)
Advancements in embryo culture, blastocyst biopsy techniques, 24-chromosome aneuploidy screening platforms, and improved genomic coverage of new sequencing platforms, such as next-generation sequencing, have made PGT safe and accessible for all patients who undergo in vitro fertilization. (medscape.com)
[ 2 ] This technique has evolved throughout the years and is now largely performed by biopsy of the blastocyst trophectoderm cells with analysis using techniques such as next-generation sequencing (NGS) and comparative genomic hybridization (CGH) to test for aneuploidy. (medscape.com)
To further investigate the genomic diversity among this group and to help characterize lineages of the plague organism that have no sequenced members, we present here the genomes of two isolates of the "classical" antiqua biovar, strains Antiqua and Nepal516. (asm.org)
Total genomic DNA was extracted from dried leaf tissue using as DNeasy® 96 Plant Kit (Cat. (uni-goettingen.de)
EC 2.1.1.6) was determined from rat cDNA and genomic libraries were screened with DNA probes and specific antiserum. (lu.se)

The rpoB gene target sequence was amplified using mutagenesis forward and reverse primers followed by HindIII enzyme digestion. (who.int)
Sequencing was performed with both the forward and reverse primers. (uni-goettingen.de)

The results showed that those 2 isolates were multi-drug resistant and the DNA sequencing analysis showed that the alignment of nucleic acid of DNA in isolates of mycobacteria other than M. tuberculosis was different from that of M. tuberculosis complex. (who.int)
DNA from the isolates was extracted and the 16S rRNA gene was amplified using primers specific for Actinomycetes. (scirp.org)
Within L. monocytogenes , sequences homologous to gtcA were found in all serogroup 4 isolates but not in strains of any other serotypes. (asm.org)
Several techniques based on the polymerase chain reaction (PCR) to facilitate the characterization and phylogenetic analysis of variola virus isolates were described. (who.int)
A total of 32 strains with one or more desirable biochemical properties were pre-selected and identified using rep-PCR fingerprinting in combination with 16S rRNA sequencing of representative rep-PCR cluster isolates. (who.int)

Total 38 nucleotides and 36 proteins sequences of linA and linB were used for constructing the phylogeny. (ispub.com)
Besides that, we offer nucleotides, buffers, DNA enhancers and PCR Cleanup, which may be one of the most important steps in a PCR reaction. (isogen-lifescience.com)

Furthermore, in-silico evaluation of primers, especially those for amplification of archaeal 16S rRNA gene regions, does not necessarily reflect the results obtained in experimental approaches. (frontiersin.org)
Overall our results indicate a rather limited comparability between community structures investigated and determined using different primer pairs as well as between metagenome and 16S rRNA gene amplicon based community structure analysis. (frontiersin.org)
In this study, we investigated fecal 16S rRNA gene sequencing analysis of changes in the gut microbiota of rats with LDA-related intestinal injury. (hindawi.com)
The maxillary arcade of each dog was sampled with a sterile swab, and swabs were submitted for next-generation DNA sequencing targeting the V1-V3 region of the 16S rRNA gene. (avma.org)

Recently, metagenomics a new approach to identify culturable and non culturable bacteria with the help of 16S rDNA sequences isolate total DNA from HCH contaminated soil and amplified 16S rDNA through universal primers and confirmed its total population with denaturing gradient gel electrophoresis and sequencing. (ispub.com)
The data were collected from capillary electrophoresis on the ABI Prism 3100® Genetic Analyzer with the Sequence Analysis Software v3.1 (Applied Biosystems). (uni-goettingen.de)

OPRM1 A118G was screened by DNA sequence analysis of polymerase chain reaction (PCR)-amplified DNA. (spandidos-publications.com)
Polymerase Chain Reaction Combined with Restriction Enzyme Analysis for Detection and Differentiation of Fowl Adenoviruses. (vin.com)
All regions were amplified by the polymerase chain reaction (PCR) using universal primers. (uni-goettingen.de)

Consequently, established primers specifically amplifying the archaeal 16S ribosomal gene region are scarce compared to the variety of primers targeting bacterial sequences. (frontiersin.org)
Epigenetics describes the study of heritable changes in gene function that occur without a change in the nuclear DNA sequence. (qiagen.com)
Molecular tools such as PCR, gene probing, mRNA transcript analysis, and DNA sequencing can aid in this endeavour. (southalabama.edu)
We will utilize several computational strategies to identify the genome difference between Lso haplotypes, including whole genome comparisons, ortholog analysis, gene neighborhood analysis, phylogenetic inference and conservation analysis. (usda.gov)
The amplified gene was sequenced and phylogeny analysis was done. (scirp.org)
We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. (scielo.br)
Translated partial amino acid sequence of pln A gene in MCC4246 displayed 48 amino acid sequences showing 100% similarity with plantaricin A of Lactobacillus plantarum (WP_0036419). (researchsquare.com)
PCR using viral DNA and primers from conserved regions of the fowl adenoviral hexon gene 3 produced an approximately 900 base pair fragment and preliminary analysis of the amplified DNA sequence suggests a novel adenovirus. (vin.com)
Highly enriched cell populations were obtained and subjected to gene expression analysis. (ku.dk)
Gene reduction analysis revealed that the gene deletion processes are under selective pressure, and many of the inactivations are probably related to the organism's interaction with its host environment. (asm.org)
Employing the DNA blot analysis only one COMT-encoding gene was found in the rat genome. (lu.se)

Molecular phylogeny of tribe Dipterocarpeae (family Dipterocarpaceae) based on sequence data of chloroplast and nuclear DNA. (uni-goettingen.de)
2006. Molecular phylogeny of Dipterocarpaceae in Indonesia based on chloroplast DNA. (uni-goettingen.de)

however, one primer set did produce a specific PCR product for both strains. (usda.gov)
Further analysis of additional E. ictaluri strains confirmed that this DNA sequence is a common feature of the E. ictaluri genome and its presence predates use of the live E. ictaluri vaccine. (usda.gov)
WHO Collaborating Centre for Orthopoxvirus Diagnosis and Repository for Variola Virus Strains and DNA, State Research Centre of Virology and Biotechnology "VECTOR", Koltsovo, Novosibirsk Region, Russian Federation. (who.int)
The Committee concluded that additional work may be needed to assess the viability of the stocks held in VECTOR, and that further molecular characterization of additional strains may be valuable in helping to identify strains from which further DNA sequences could be determined. (who.int)
These strains were further investigated for clonal relationships, using RAPD-PCR with three primers, and of the 32 a total of 16 strains were finally selected for the development as starter cultures for Gari production. (who.int)

Fungal rRNA sequencing of indoor and occupational air samples. (cdc.gov)
For traditional microscopic analyses, spores were extracted from samples and stained with lactophenol cotton blue. (cdc.gov)
The fecal samples were then analyzed using high-throughput sequencing of 16S rRNA V3-V4 amplicons. (hindawi.com)
He has been working on development and support of pipelines for processing and analyzing genome sequencing data for thousands of samples on JCVI High-throughput compute facility and AWS cloud. (jcvi.org)
QIAseq Targeted Methyl Panels deliver high mapping efficiency (figure QIAseq Targeted Methyl Panel: mapping efficiency Input >1 ng – gDNA – FFPE – ccfDNA Results show high mapping on primer and high unique mapping. "> QIAseq Targeted Methyl Panel: mapping efficiency ), high reproducibility (figure QIAseq Targeted Methyl Panel: methylation degree reproducibility To test methylation degree accuracy and reproducibility, 40 ng control DNA with 0%, 50% and 100% methylation were processed using the QIAseq Targeted Methyl Panel, which consisted of 749 primers. Samples were sequenced on MiSeq and methylation degree of CpG covered by >30 UMI was evaluated, resulting in accurate and highly reproducible methylation calling. "> QIAseq Targeted Methyl Panel: methylation degree reproducibility ) and high correlation with established methods (figures QIAseq Targeted Methyl Panel - high correlation with established methods To test correlation with established methods, the QIAseq Targeted Methyl Panel was used with 40 ng input gDNA from hepatocytes. The panel consisted of 102 primers, covering 71 CpGs. Compared to the Supplier I array, methylation degree resulted in high correlation. "> QIAseq Targeted Methyl Panel - high correlation with established methods and QIAseq Targeted Methyl Panel: methylation status of FFPE DNA: methylation degree To test methylation status of FFPE DNA, the QIAseq Targeted Methyl Panel was used with 40 ng input FFPE DNA. The panel consisting of 93 primers covered 566 CpG sites. The evaluated methylation degree of 7 CpG sites of the MGMT gene highly correlates to the methylation degree that was previously validated with Pyrosequencing. "> QIAseq Targeted Methyl Panel: methylation status of FFPE DNA: methylation degree ). (qiagen.com)
A PCR assay using 553V-specific oligonucleotide primers designed to screen for viruses in field samples is under development. (vin.com)
Three samples of each tissue type were used for the analyses. (ku.dk)

In the last two decades, DNA barcoding, the sequencing of short species-specific amplicons, has considerably simplified community analyses (Hebert et al. (springer.com)
Amplicons were cloned, sequenced, and used in BLAST searches against the NCBI nucleotide database to determine the closest fungal relative based on sequence similarity. (cdc.gov)

Development of data analysis tools and pipelines for genome analysis. (jcvi.org)

Researchers can perform restriction enzyme analyses, edit sequences, create plasmid maps, cut and ligate "in silico," analyze GC content, make homology plots, and select primer sites from a user-specified database. (the-scientist.com)
The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)

These ribosomal sequences was functional for the phylogenetic analysis and molecular taxonomic of any bacteria. (ispub.com)

In forensics, mutation rates are needed when STRs are applied to paternity testing, and Y-STRs are especially powerful in deficiency cases of disputed paternity involving male offspring where the alleged father is not available for DNA analysis but is replaced by any of his male paternal relatives. (springer.com)
Of these, studying DNA-confirmed father-son pairs is the most reliable approach but only if the number of father-son pairs investigated is large enough to reveal reliable mutation rate estimates. (springer.com)
Sequencing and alignment of this region in 11 cancer cell lines and 4 normal cell lines were also performed, followed by cloning, mutation and luciferase assay analysis. (jcancer.org)
Approximately 80% of patients with CS, 60% patients with BRRS, 20% of patients with PS/PSL syndrome will have a PTEN mutation identifiable by sequencing. (cincinnatichildrens.org)
Deletion/duplication analysis of PTEN will detect a mutation in about 10% of individuals with Bannayan-Riley-Ruvalcaba syndrome. (cincinnatichildrens.org)
An analysis of the empirical fitness landscape revealed mutation-by-mutation-by-host-genotype interactions that demonstrate coevolution modified the contours of λ's landscape. (elifesciences.org)
By coupling sequencing and pairwise competition experiments, we demonstrated that the first mutation λ evolved en route to the innovation would only evolve in the presence of the ancestral host, whereas later steps in λ's evolution required the shift to a resistant host. (elifesciences.org)

it is generally believed that all the copies in an organism are identical or nearly identical in nucleotide sequence. (ispub.com)

As we recently reported, analysis of 77 FHWT by whole genome sequencing (WGS, 58 patients) or whole exomic sequencing (WES,19 patients) identified 825 high-quality somatic, non-synonymous variants, with an average of 11 candidate mutations/case (range 2-42) (ref. 6 ). (nature.com)

restriction site searches, primer and probe design, sequence analyses etc. (bio.net)

The cytopathic effect was suggestive of an adenovirus and molecular analyses were performed to further characterize the virus isolate 553V. (vin.com)

2003 ). DNA barcodes can provide information on genetic variation within and between species, rapidly assign taxonomic status across divergent lineages (Hebert and Gregory 2005 ), and identify the prey composition of predators' gut contents (Agustí et al. (springer.com)

We compare all five currently sequenced Y. pestis genomes and the corresponding features in Yersinia pseudotuberculosis . (asm.org)

While there are numerous NUMTs existing in the nuclear DNA, the primers we use for mtDNA enrichment is highly specific to avoid any potential amplification of NUMTs from nuclear DNA. (northwestern.edu)
Phylogenetic analysis with DNA amplification technologies. (who.int)

We here use a purified recombinant mitochondrial transcription system and demonstrate that conserved sequence box II is a sequence-dependent transcription termination element in vitro. (nih.gov)

Standard mtDNA-seq bioinformatics service includes sequencing data QC, alignment to the reference mitochondrial genome, and variant analysis. (northwestern.edu)
For multiple alignment, the BioEdit Sequence Alignment Editor version 7.0.5.3 (Hall 1999) was used and the phylogenetic analysis was carried out with PAUP* v4.0b10 (Swofford 1998). (uni-goettingen.de)

The human mitochondrial transcription machinery generates the RNA primers needed for initiation of heavy strand DNA synthesis. (nih.gov)
Transcription from the light strand promoter is prematurely terminated at positions 300-282 in the mitochondrial genome, which coincide with the major RNA-DNA transition points in the D-loop of human mitochondria. (nih.gov)
Because of the increased awareness on the importance of metabolism and bioenergetics in a wide variety of human diseases, more and more mitochondrial DNA (mtDNA) studies are performed to study the link between mtDNA sequence variation and disease development. (northwestern.edu)
Nearly all DNA extraction kits or procedures purify both nuclear and mitochondrial DNA into the final product. (northwestern.edu)
Does the long-range PCR performed to enrich mtDNA from total gDNA amplify NUMTs (nuclear mitochondrial DNA sequences)? (northwestern.edu)

It provides comprehensive market analysis, highlighting the escalating use of oligonucleotide synthesis in diverse fields ranging from human health management to molecular diagnostics and therapeutics. (researchandmarkets.com)
This can be attributed to the large revenue share of oligonucleotide synthesis for production of PCR primers. (researchandmarkets.com)

The possible hypoxia response element (HRE) sequences in 5' non-coding Region before ATG start codon of Sema4D were screened, followed by point mutagenesis and luciferase assay analysis. (jcancer.org)

DNA extracted from potato psyllid specimens containing either Lso haplotype A or B will be used as a control to verify that the primers and PCR thermal cycler conditions are successful. (usda.gov)
The objective of this document is to outline the available methods and tools for screening and sequencing patient specimens for the detection of VOC or VOI, with a focus on those that offer a rapid turn-around time so as to support rapid implementation of public health actions for COVID 19 control. (who.int)

The sensitivity of DNA sequencing is over 99% for the detection of nucleotide changes, small deletions and insertions in the regions analyzed. (cincinnatichildrens.org)
DNA, serological detection of variola virus, antiviral agents and animal models of smallpox. (who.int)

DNA meth-ylation represses transcription by interfering with transcription factor binding and indirectly by recruiting methyl-CpG-binding proteins and reducing chromatin remodeling activities ( 12 ). (spandidos-publications.com)

Three archaeal 16S primer pairs as well as two bacterial and one general microbial 16S primer pairs were comprehensively tested by in-silico evaluation and performing an experimental analysis of a complex microbial community of a biogas reactor. (frontiersin.org)
The primers are in silico and experimentally validated, and used by the mtDNA research community. (northwestern.edu)

In the latter, archaeal primer pair ArchV34 showed the highest similarity to the archaeal community structure compared to observed by the metagenomic approach and thus appears to be the appropriate for analyzing archaeal communities in biogas reactors. (frontiersin.org)

The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human GAPDH Exon 1 Primers #5516 and SimpleChIP ® Human α Satellite Repeat Primers #4486. (cellsignal.com)

The primers for trnL intron and trnL-trnF intergenic spacer regions were described in Taberlet et al. (uni-goettingen.de)

In addition to RNA-associated silencing and histone modification, a major epigenetic mechanism in higher-order eukaryotes is DNA methylation. (qiagen.com)
DNA methylation occurs on cytosine residues, especially in CpG islands, which are GC-rich regions. (qiagen.com)
QIAseq Targeted Methyl Panels enable Sample to Insight, targeted next-generation sequencing (NGS) to interrogate DNA methylation degree. (qiagen.com)
Moreover, in the ketamine group, the level of Ten‑Eleven‑Translocation methylcytosine dioxygenase for demethylation as determined by reverse transcription‑quantitative PCR assay was increased in comparison with the control group, but that was not the case for the level of DNA methyltransferases for methylation. (spandidos-publications.com)
The mechanism of epigenetic regulation involves the CpG site methylation of promoter regions and the modification of DNA and histones by altering chromatin structure ( 9 - 11 ). (spandidos-publications.com)
Moreover, the CpG site methylation is mediated by DNA methyltransferases (DNMTs), which catalyze the addition of a methyl group to cytosine ( 13 , 14 ). (spandidos-publications.com)
In contrast, the Ten-Eleven-Translocation (TET) dioxygenase family, including TET1, TET2 and TET3, mediates active DNA demethylation and hydroxylate-methylated DNA by converting 5-methylcyto-sine to 5-hydroxymethylcytosine to regulate DNA methylation status. (spandidos-publications.com)

The predicted size of the COMT transcript was 1.8-2.0 kb, which could be confirmed from Northern hybridization analyses of the isolated rat liver mRNA. (lu.se)

Primer extension analysis showed that the transcription start point of the rat liver COMT mRNA was 450 bp upstream from the. (lu.se)

The sequencing reactions were performed by using ABI Prism™ Big Dye™ Terminator Cycle Sequencing Ready Reaction Kit v1.1 (Applied Biosystems). (uni-goettingen.de)

Moreover, DNA hypomethylation of the COX‑2 promoter region located from ‑1,522 to ‑829 bp might contribute to transcriptional regulation of COX‑2 expression and induce a pro‑inflammatory response in KIC. (spandidos-publications.com)
The enriched DNA was quantified by real-time PCR using human c-Myc promoter primers, SimpleChIP ® Human H19/Igf2 ICR Primers #5172, and SimpleChIP ® Human α Satellite Repeat Primers #4486. (cellsignal.com)
Promoter analysis of PTEN will identify mutations in about 10% of patients with Cowden syndrome. (cincinnatichildrens.org)

CONCLUSIONS: Compared to traditional methods of analysis, our data demonstrate a much wider diversity of fungi that may possibly contribute to personal exposure and adverse health effects in home and occupational environments. (cdc.gov)
The next-generation DNA sequencing used in the present study revealed substantially more species of fungi than previous culture-based studies. (avma.org)

However, a disadvantage of this primer pair was its low specificity for the archaeal domain in the experimental application leading to high amounts of bacterial sequences within the dataset. (frontiersin.org)
This approach involves isolating bacterial DNA directly from the environment and making a library of that environment. (southalabama.edu)
PCR using the new primer pair on a set of template DNAs of different bacterial genera showed that the method was selective for bacteria belonging to the family Sphingomonadaceae . (ispub.com)

Sequencing system used is Applied Biosystems ABI3730XL DNA Analyzer. (helsinki.fi)

The results obtained clearly demonstrate that comparability of community profiles established using different primer pairs is difficult. (frontiersin.org)
The results showed that there were four possible HREs (HRE1-4) sequences in 1275bp range before ATG start codon. (jcancer.org)
As each mitochondrion contains 2-10 copies of mtDNA and each cell contains hundreds to thousands copies, the presence of heterogeneous mtDNA sequences, due to different mutations in different copies, results in a condition called heteroplasmy. (northwestern.edu)
Raw data and analysis results are usually returned to user via OneDrive . (northwestern.edu)
Sequencing results will be further incorporated into comparative genomics and phylogenetic analyses (approaches in Objective 1) to correlate genotypic data with our phenotypic knowledge. (usda.gov)
Rare primer site variants may lead to erroneous results. (cincinnatichildrens.org)

A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. (scielo.br)
DNA fragments larger than 4 kb should be purified using the QIAquick PCR Purification Kit. (qiagen.com)

We discuss amplicon-based DNA barcoding and metabarcoding for the analysis of community diversity and molecular gut content analysis for assessing predator-prey relationships. (springer.com)

The identity of the clone and the accuracy of the sequence was verified by direct aa sequencing of the tryptic peptides derived from the purified rat liver enzyme. (lu.se)

Therefore, we investigated the 17 Yfiler Y-STRs in 1,730-1,764 DNA-confirmed father-son pairs per locus and found 84 sequence-confirmed mutations among the 29,792 meiotic transfers covered. (springer.com)
Our finding of three Y-STR mutations in one father-son pair (and two pairs with two mutations each) has consequences for determining the threshold of allelic differences to conclude exclusion constellations in future applications of Y-STRs in paternity testing and pedigree analyses. (springer.com)

The application of next-generation sequencing technology in microbial community analysis increased our knowledge and understanding of the complexity and diversity of a variety of ecosystems. (frontiersin.org)
In this study, we aimed to validate archaeal primers suitable for high throughput next generation sequencing. (frontiersin.org)

In contrast to Bacteria, the archaeal domain was often not particularly addressed in the analysis of microbial communities. (frontiersin.org)
I use primers and probes to key genes in the degradation pathways of various environmental contaminants to determine how the microbial community as a whole responds to that contaminant. (southalabama.edu)
2013. Microbial community analysis of Deepwater Horizon oil-spill impacted sites along the Gulf coast using functional and phylogenetic markers. (southalabama.edu)

In addition to DNA sequencing, small to medium scale fragment analysis service is also available. (helsinki.fi)
As a starting material we use purified PCR products mixed with sequencing primer or ready made sequencing/fragment analysis reactions. (helsinki.fi)
After sample dropoff, core staff conducts sample QC, which includes Qubit concentration measurement and Bioanalyzer fragment analysis, prior to mtDNA enrichment and library construction. (northwestern.edu)

PCR primers are short and a small-scale synthesis is sufficient for a large number of reactions. (researchandmarkets.com)

This, coupled with the very limited DNA damage repair capability in the organelle, leads to sequence alterations in mtDNA at a much higher frequency than that in the nuclear DNA. (northwestern.edu)
Virtually all DNA extractions contain both nuclear DNA and mtDNA. (northwestern.edu)
NUMTs are a result of insertion of mtDNA sequence into the nuclear genome. (northwestern.edu)
Many studies have been carried out to resolve the taxonomic relationships of subfamily Dipterocarpoideae based on chloroplast and nuclear DNA. (uni-goettingen.de)

We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)

The ARX and CDKL5 genes, both of which are located within the Xp22 region, were sequenced in the affected family members, and a deletion of nucleotide 183 of the coding sequence (c.183delT) was identified in CDKL5 in the affected family members. (nih.gov)
We will also utilize the domain-centric protein sequence and structural analyses to understand the potential functions of the identified genes. (usda.gov)
Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)

The MinElute PCR Purification Kit contains a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. (qiagen.com)
Analysis of PCR products before ( b ) and after ( a ) purification with the MinElute PCR Purification Kit. (qiagen.com)

Methods: This qualitative study was performed using content analysis approach between May-July 2022. (bvsalud.org)

Most DNA replication events from the heavy strand origin are prematurely terminated, forming a persistent RNA-DNA hybrid, which remains annealed to the parental DNA strand. (nih.gov)
Based on our findings, we propose a model for primer formation at the origin of heavy strand DNA replication. (nih.gov)

Northern analysis detected a specific 2.5-kilobase pair hOAT1 transcript only in human kidney. (aspetjournals.org)

As with targeted DNA panels, QIAseq solutions are based on single-primer extension (SPE) technology that provides a sensitive and specific solution for detecting regions all across the genome. (qiagen.com)

In this pilot study, rRNA sequencing was utilized to examine the diversity of fungal species present in indoor and occupational environments. (cdc.gov)
Standard PCR using a high fidelity, proof reading polymerase will then be conducted using DNA previously isolated from Lso-infected non-potato psyllid species collected in the Klamath Basin of Oregon. (usda.gov)
The analysis by telomeric fingerprinting showed polymorphism between both species and the chromosome minimal numbers estimated were three. (scielo.br)

Anatomy6

Organisms22

Chemicals and Drugs76

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Phenomena and Processes98

Disciplines and Occupations6

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture2

Information Science9

Health Care10

Geographicals2

Sanger3

Genomic12

Forward and reverse primers2

Isolates5

Nucleotides2

RRNA gene4

Electrophoresis2

Polymerase chain re3

Gene11

Phylogeny2

Strains5

Samples7

Amplicons2

Genome Analysis1

Plasmid2

Ribosomal1

Mutation7

Nucleotide sequence1

Whole genome1

Restriction1

Isolate1

Genetic1

Genomes1

Amplification2

Recombinant1

Alignment2

Mitochondrial5

Oligonucleotide2

Mutagenesis1

Specimens2

Detection2

Proteins1

Silico2

Similarity1

Exon1

Intron1

Methylation7

Hybridization1

MRNA1

Reaction1

Promoter3

Fungi2

Bacterial3

Analyzer1

Results6

Fragments2

Amplicon-based1

Enzyme1

Mutations2

Next generation2

Microbial3

Fragment analysis3

Synthesis1

Nuclear4

Viral1

Genes3

Purification2

20221

Replication2

Transcript1

Specific1

Species3