The First Strand cDNA Synthesis Kit is used for the synthesis of the first strand cDNA as the starting reaction for two-step RT PCR.The kit includes Reverse Transcriptase AMV for first strand synthesis, two different primers, our PCR Nucleotide Mix, and Control Neo pa RNA. (sigmaaldrich.com)
Maxime™ RT PreMix Kit (Oligo dT15 Primer) is a product that makes cDNA synthesis simple and fast from RNA of eukaryotic cells. (labotaq.com)
As a result, the Maxime™ RT PreMix Kit (Oligo dT15 primer) showed equivalent performance to the existing manual type, Power cDNA Synthesis Kit, and the sensitivity was also comparable to Maxime™ RT PreMix Kit (Random primer). (labotaq.com)
The purified mRNA was fragmented through the RNA fragmenta tion kit and utilized as template for very first strand cDNA synthesis making use of random hexamer primers and reverse transcriptase. (sykinhibitors.com)
UltraScript™ cDNA Synthesis Kit Separate Oligos, UltraScript Reverse Transcriptase, or UltraScript 2.0 Reverse Transcriptase should be used because they come with a buffer that does not contain random hexamers and oligod(T)s, which could interfere with miRNA specific primers. (pcrbio.com)
Use universal RT primers and add poly(A) or poly(U) tails (e.g. by poly(U)-polymerase), followed by cDNA synthesis using universal primers 1,2 . (pcrbio.com)
Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) is an RNA-dependent DNA polymerase that can be used for cDNA synthesis and subsequent PCR or qPCR in one-step or two-step RT-PCR or RT-qPCR assays. (meridianbioscience.com)
For general cDNA synthesis, random hexamer primers and oligo(dT) primers can be used either separately or mixed. (meridianbioscience.com)
Synthesis4
Tumor virus ribonucleic acid directed deoxyribonucleic acid synthesis: nucleotide sequence at the 5' terminus of nascent deoxyribonucleic acid. (wikidata.org)
Oligo dT has been used to prime the synthesis of the first strand using Moloney Murine leukemia Virus (MMLV) Reverse Transcriptase. (genelink.com)
HTBS recruits HIV reverse transcriptase, which nucleates DNA synthesis and is aided in elongation by murine leukemia reverse transcriptase. (nature.com)
Yes, to initiate reverse transcription, the MMVL-RT reverse transcriptase require a primer to bind to its complementary sequences on the RNA template and serve as a starting point for synthesis of a new strand. (meridianbioscience.com)
Strand4
Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. (cathdb.info)
Sequence complementary to the nicked genomic DNA strand serves as a primer binding site (PBS). (addgene.org)
This virus has a bipartite positive-strand RNA genome consisting of RNA1 and RNA2, which are separately encapsidated in isometric particles of 28 nm in diameter. (ac.ir)
The First cDNA strand was synthesized using antisense primer (SqMV-CPR) and Moloney murine leukemia virus (MMuLV) reverse transcriptase (Parstoos, Iran). (ac.ir)
Inhibitor1
After chilling on ice, the next components had been put into the examples: 5 invert transcriptase buffer, 2.5 mM dNTPs, RNase inhibitor, and Moloney Murine Leukemia Virus invert transcriptase. (careersfromscience.org)
Oligo5
Maxime™ RT PreMix Kit (Oligo dT15 Primer) contains all components (Oligo dT15 Primer, dNTP mixture, Reaction buffer, etc.) required for RT reaction including OptiScript™ RT System, and it is contained in each PCR tube with dried pellet state by ALHP system. (labotaq.com)
This product contains oligo dT15 primer, so it is easy to apply to eukaryotic RNA with poly (A) tail. (labotaq.com)
The RNA extracted from human cell (SNU-1) was serially diluted at intervals from 1ug to one-third level, and cDNA was synthesized using Maxime™ RT PreMix Kit (Oligo (dT)15 Primer). (labotaq.com)
Maxime™ RT PreMix (Oligo (dT)15 Primer) is a product that keeps the performance of the product for 1 year under the freezing storage condition by preventing oxidation and incorporation of moisture by the vacuum compression manufacturing method. (labotaq.com)
We advise using specific primers or oligo(dT) and optimising each aspect of the reaction, for example, the different enzyme concentrations, primers, primer concentration, temperature and temperature cycling and time. (pcrbio.com)
The complete length of the coat proteins (CPL, CPS) gene was amplified using specific primers SqMV-CPF by Taq master mix red (Amplicon, Denmark). (ac.ir)
Whole-amplicon interrogation with HRM revealed insertions, deletions, and heterozygotes in secondary oat germplasm pools, generating multiple alleles at some primer targets. (biomedcentral.com)
This method uses unlabeled primers and interrogates the entire amplicon, providing an efficient SNP genotyping system in terms of reagent cost, throughput, and data production. (biomedcentral.com)
Amplify3
The PCR conditions to amplify the target cDNA will be based on the primers selected. (genelink.com)
It should be noted that specific sequence primers as well as degenerate sequence primers can be used successfully to amplify the target sequence. (genelink.com)
These primers amplify the complete CPL and CPS genes of SqMV with 1900 bp length. (ac.ir)
RNA isolation and qPCR Total cellular RNA was extracted from 3T3-L1 cells and mice epididymal fat pads utilizing TRIzol reagent as previously reported.50,51 The cDNA was synthesized from 1 g of total RNA in 20 L working with random primers and Moloney murine leukemia virus reverse transcriptase. (acthreceptor.com)
Predominant1
The purpose of this study was to characterise the multigenic structure of the sarcomeric MHC family in the guinea pig, and to design and validate specific oligonucleotide primers to enable the assessment of the predominant adult-muscle associated MHC mRNAs in relevant disease models. (biomedcentral.com)
Enzyme1
Norwegian ticks were tested for TBE virus (TBEV) RNA by from both patients by enzyme immunoassay (Enzygnost, reverse transcription-polymerase chain reaction. (cdc.gov)
Reverse4
This is achieved through the use of a reverse transcriptase such as AMV RT (avian myeloblastis virus reverse transcriptase) or M-MuLV RT (moloney murine leukemia virus reverse transcriptase). (sigmaaldrich.com)
This primer editor protein consists of a Cas9 nickase fused to a reverse transcriptase. (addgene.org)
RNA samples (two mg) had been then reverse-transcribed with Moloney murine leukemia virus reverse transcriptase (New England Biolabs) and real-time PCR performed using SYBR Green (TOYOBO) plus a common curve for quantization, as described previously [23]. (dot1linhibitor.com)
PegRNA1
To optimize pegRNAs, the team found that extending the pegRNA primer binding site to at least eight nucleotides enabled more efficient prime editing in HEK293T cells. (addgene.org)
Specificity1
Specific oligonucleotide primers were designed, and following verification of their specificity, found to successfully determine the expression of each MHC mRNA independently. (biomedcentral.com)
Protein1
Primers were designed for canine distemper virus nucleocapsid protein coding region fragment amplification (84 bp). (scielo.br)
Avian2
A study of the relationship of reticuloendotheliosis virus to the avian leukosis-sarcoma complex of viruses. (wikidata.org)
Avian reticuloendotheliosis virus (strain T): V. DNA polymerase. (wikidata.org)
Denmark1
PCR amplicons were purified and sequenced in Norway and Denmark is caused by TBEV, but virus has by using a DNA sequencing kit (ABI Prism, PE not been isolated from humans in these countries. (cdc.gov)
Nucleotides1
3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus. (cathdb.info)
Specific5
at a site determined by a sequence-specific primer. (sigmaaldrich.com)
As such, the MHC mRNAs, and specific oligonucleotide primers of all common laboratory species have been available for some time. (biomedcentral.com)
The publication of these specific oligonucleotide primers for the guinea pig MHC variants will enable researchers to rapidly and accurately quantify acute changes in MHC mRNA expression in either developmental or in guinea pig disease models where a marker of altered skeletal muscle function is required. (biomedcentral.com)
Use specific RT primers and omit the tailing step 1,3-5 . (pcrbio.com)
We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
The global epidemiology of Hepatitis C Virus (HCV) may be roughly described by two groups of genotypes: the worldwide distributed ones (subtypes 1a, 1b, 2a and 3a, among others) and the endemic ones (subtypes 4a, 5a, 6a, among others). (cdc.gov)
Citation: Culasso ACA, Elizalde M, Campos RH, Barbini L (2012) Molecular Survey of Hepatitis C Virus in the Touristic City of Mar Del Plata, Argentina. (cdc.gov)
The purpose of this research was to determine if particular viral co-infections by individual immunodeficiency virus (HIV), human T cellular lymphotrophic virus type 1 (HTLV-1), hepatitis B virus (HBV) and hepatitis C virus (HCV) are connected with leprosy unfavorable outcomes. (researchensemble.com)
Site1
Arezi and Hogrefe, 2009 ), the Liu lab created and evaluated 19 PE1 variants with RT mutations known to increase activity, enhance binding between the template and primer binding site, increase processivity, or improve thermostability. (addgene.org)
Reaction2
A quantitative real time polymerase chain reaction (PCR) revealed canine distemper virus presence in peripheral blood samples from asymptomatic and non vaccinated dogs. (scielo.br)
The real-time PCR reaction mixture consisted of ten ml of 26SYBR green Master Mix, 0.five ml of ten pM primers, and two ml of cDNA in a total volume of 20 ml. (dot1linhibitor.com)
Type1
Present in human immunodeficiency virus type 1. (cathdb.info)
General1
Ogle and TAM amplicons from 12 primers were sequenced for SNP validation, revealing complex polymorphism in seven amplicons but general sequence conservation within SNP loci. (biomedcentral.com)
Positive3
Canine distemper virus vaccine samples in VERO cells were used as positive controls. (scielo.br)
Quantitative results of real time PCR generated by ABI Prism 7000 SDS Software showed that 54.5% of dogs with asymptomatic canine distemper were positive for canine distemper virus. (scielo.br)
However, the reconstruction of the population dynamics is a positive RNA virus [1] that currently infects approximately 3% better tool to establish whether HCV infections are either in a of the world's population [2]. (cdc.gov)