Primary perform assays and flow cytometry image cytometry. Medical search. Frequent questions

The aim of this work was to study invasion dynamics in GBM using an innovative in vivo imaging approach. (nature.com)
To further investigate the physiological processes 40 underlying GBM cell movement, this study aimed to image and analyze distinct GBM invasive growth patterns found in vivo , similar to those observed in patients. (nature.com)
Cell morphology was microscopied with HE staining and an in vivo imaging system was performed to detect the migration and lung-specific metastasis. (magtechjournal.com)
in vivo imaging results validated that the fluorescent signal was mainly concentrated in the lung after injection of 4T07LuM cells labeled with luciferase in situ . (magtechjournal.com)
In addition, the feasibility of imaging of DAbR1-positive T cells in vivo after intravenous injection of 86 Y/ 177 Lu-AABD was studied and radiation doses determined. (snmjournals.org)
Imaging studies showed 86 Y-AABD was retained by DAbR1-positive T cells while it continuously cleared from normal tissues, allowing for in vivo tracking of intravenously administered CAR T cells. (snmjournals.org)
IRDye 680LT Secondary Antibodies are not intended for use in In-Cell Western ™ Assays or in vivo imaging applications. (licor.com)
A Met-directed imaging agent was engineered by labeling Met-specific onartuzumab with zirconium-89 (Zr-89) and its in vivo performance was evaluated in subcutaneous and orthotopic PDAC xenograft models. (thno.org)
Cell functional assays, in vivo xenografts tumor growth experiments, and survival analysis were used to investigate the functional significance and mechanism of chemosensitization after SETD8 downregulation. (biomedcentral.com)
In vitro T cell-mediated killing assays and in vivo syngeneic mouse models were used to investigate the functional roles of circIGF2BP3 and its downstream target PKP3 in antitumor immunity in NSCLC. (biomedcentral.com)
In vivo , orthotopic tumor xenografts assay was performed to investigate the role of C1QBP in RCC progression. (medsci.org)

Wound healing and Transwell assays were performed to detect migration of cell lines in vitro . (magtechjournal.com)
C1QBP knockdown and overexpression cells were established via lentiviral infection and subjected to apoptosis and ROS assay in vitro . (medsci.org)
The material from each step isscreened using acellular and in vitro assays for evaluating general toxicity, mechanisms of toxicity, and macrophage function. (cdc.gov)

Cell apoptosis or cell cycle was analyzed by flow cytometry, and chromosome misalignment was observed by a fluorescent microscopy. (biomedcentral.com)
Flow cytometry was used to examine apoptosis, cell cycle distribution, and accumulation of reactive oxygen species (ROS). (jcancer.org)
MTT analysis, PI/Annexin V staining, transwell and metabolic flux assays were performed to examine the effects of C1QBP on proliferation, apoptosis, migration, invasion, and oxidative phosphorylation of HCC cells. (jcancer.org)
Loss-of-function assays showed that proliferation, migration and invasion of HCC cells were mitigated while cell apoptosis was augmented upon the loos of C1QBP. (jcancer.org)

In addition, according to the results of Transwell and wound healing assays, 4T07LuM, compared to the parental cells 4T07, had shown a significantly more invasive migration( P ＜0.001). (magtechjournal.com)
The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)

After irradiation, the expression of DDR-related genes and proteins in ΔNp63α-expressing and control cells was analysed by RT-qPCR, Western blotting, and flow cytometry. (researchsquare.com)

Astrocytes were transfected with siRNA directed to GW182 and hAgo2 and unlike control astrocytes, a primary cilium was no longer associated with the centrosome as detected in indirect immunofluorescence assays. (biomedcentral.com)
We assessed cell membrane Met levels in human patient samples and pancreatic ductal adenocarcinoma (PDAC) cell lines (BxPC3, Capan2, Suit2, and MIA PaCa-2) using immunofluorescence, flow cytometry and cell-surface biotinylation assays. (thno.org)

The initial resistance that the high-content/high-throughput community had to face has been overcome, and now most in the field acknowledge that flow cytometry is enormously high throughput and very high content. (genengnews.com)
Moreover, inclusion of a high throughput imaging cytometer allows for many of these same comparisons to be made without the need for trypsinization and extensive cell processing. (utsouthwestern.edu)
The Celigo is a bench-top, high throughput, micro-well plate based, bright field and fluorescent imaging system. (utsouthwestern.edu)

In certain circumstances, when a lymph node is not easily accessible for excisional or incisional biopsy, a combination of core biopsy and FNA biopsies in conjunction with appropriate ancillary techniques for the differential diagnosis (immunohistochemistry, flow cytometry, PCR for IGHV and TCR gene rearrangements, karyotype, and fluorescence in situ hybridization [FISH] for major translocations) may be sufficient for diagnosis. (medscape.com)

Cells viability was detected by Cell Counting Kit 8 (CCK8) assays. (azd8931inhibitor.com)
Cell viability or clonogenicity was tested by violet crystal assay. (biomedcentral.com)
Collectively, both arms of this Module add scientific value to the work of vision scientists by reducing the time and effort required for basic cell culture assays such as viability and cell cycle assessments. (utsouthwestern.edu)
and a Nexcelom Cellometer for rapid determination of cell number, cell viability, transfection efficiency, and cell cycle assays. (utsouthwestern.edu)
To determine whether Met expression levels correlate with sensitivity to Met inhibition by tyrosine kinase inhibitors (TKIs), we performed cell viability studies. (thno.org)

Based on ELISA and flow cytometry, the antibody reacts with the heavy chain of mouse IgG 1 . (licor.com)

The expression levels of mRNA and proteins were detected by qPCR and Western blot, or evaluated by ﬂow cytometry. (azd8931inhibitor.com)

Flow cytometry and cell-surface biotinylation studies showed that cell-membrane Met was significantly more abundant in BxPC3, Capan2, and Suit2 when compared with MIA PaCa-2 pancreatic tumor cells. (thno.org)

He described the graphical interface the group developed-a logic map designed to analyze the data from functional live-cell assays that incorporate fluorescent markers. (genengnews.com)
DAbR1 was subcloned together with green fluorescent protein into an SFG-retroviral vector and used to transduce CD3/CD28-activated primary human T cells and second-generation 1928z (CAR) T cells. (snmjournals.org)
The flow cytometer is equipped with multiple lasers for analysis of multi-fluorescent labelled cell populations and acoustical focusing for improved cell separations. (utsouthwestern.edu)
It consists of 4 fluorescent channels (UV to far red) and has extensive applications including bright field cell counting, wound-healing and cell growth assays, cell line development, induced pluripotent stem cell (iPSC) reprogramming, 3D invasion and migration modeling, immune-oncology, virology, and fluorescent-based assays. (utsouthwestern.edu)

Flow cytometry was used to analyze the changes of immune cells in primary site, metastasis and spleen. (magtechjournal.com)
Overall the use of flow cytometry to analyze individual cell populations and evaluate the effects of drugs on cell function allows you "to ask the what-if questions" and to begin to integrate pathways, he said. (genengnews.com)

Automation of the entire process-from sample preparation, to running complex assays, to data analysis-makes it possible to perform functional phenotypic profiling of cell populations efficiently. (genengnews.com)
The goal of the MLP is to screen compounds in the Small Molecule Repository in target-based and phenotypic assays to identify and subsequently optimize small molecule s as research probes. (nih.gov)

Furthermore, flow cytometric analysis revealed an increase in apoptotic cells and a decrease in 5-ethynyl-2´-deoxyuridine uptake in sip63 cells compared to scr cells. (researchsquare.com)
Applications Reported: The WM-59 (WM59) antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
Applications Tested: The WM-59 (WM59) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
Facility provides instrumentation, technical and professional assistance, training and consultations for performing laser-based flow cytometric analysis and sorting. (nationaljewish.org)

Finally, according to the results of flow cytometry detection, 4T07LuM cell had showed significantly altered the immune cell ratios as compared to that of 4T07 cell. (magtechjournal.com)
The results of this project will be used to support the 'deep tissue Raman imaging by dynamic wavefront correction' project. (stanford.edu)
Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection. (licor.com)
Conditional logistic regression was used to compare demographics, presenting symptoms, laboratory and imaging results, treatments administered, and outcomes between cohorts. (cdc.gov)

Our lab is focused on high-sensitivity high-specificity ultrasound imaging system involving both technological advancements in the detection of bound microbubbles under ultrasound as well as the development of novel peptide ligands that can be used to formulate targeted-microbubbles. (stanford.edu)
Heterogeneity assessment, publication bias, and sensitivity analysis will be performed. (magiran.com)

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (thermofisher.com)
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. (thermofisher.com)

Further, our study demonstrates that repression of key effector proteins in the post-transcriptional miRNA pathway impairs primary cilium formation. (biomedcentral.com)
In addition to H4K20, SETD8 protein is able to perform lysine-methylations of many other cancer-related proteins. (biomedcentral.com)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)

Describing the benefits of using flow cytometry to study disease specificity as it relates to cell types and signaling pathways, Dr. Kritzik reported that it is possible to measure at least 10 parameters in 5,000-10,000 single cells per second. (genengnews.com)

The NIH Molecular Libraries Roadmap Initiative wishes to solicit HTS assay applications from the scientific community from investigators who have the interest and capability to work with the Molecular Libraries Probe Production Centers Network (MLPCN) in support of chemical probe development. (nih.gov)
The Cell Culture arm of this module provides technical expertise to investigators to assist in the establishment of primary cultures and development of new immortalized cell lines. (utsouthwestern.edu)
The NJH lab provides a resource for storage of multiple cell tissue samples, technical consultation, assay development, training for CTRC investigators, staff, visiting physicians, and students. (nationaljewish.org)

Using this approach, a biological sample can be analyzed in parts-exploiting the ability to separate individual cells for analysis using flow cytometry-with the data then combined to piece together the complete picture. (genengnews.com)
Biological assays showed that invasive and migratory behavior was strengthened by hypoxia only in GBM stem cells. (biomedcentral.com)
In addition the Core facility provides users with access to state-of -the-art instrumentation for biological imaging. (nationaljewish.org)

Using high-resolution time-lapse intravital imaging, tumor cell migration in the tumor core, border and invasive front was recorded. (nature.com)

Metabolic markers were measured in fasting serum and ATMs characterized by immunohistology, flow cytometry, and tissue culture studies. (diabetesjournals.org)

Design and Methods OPM2 and high-risk primary myeloma tumors were grown in human fetal bone implanted into non-obese diabetic severe combined immunodeficiency mice with a deficient interleukin-2 receptor gamma chain. (haematologica.org)
The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. (biomedcentral.com)

Glioblastoma (GBM) is one of the most aggressive primary brain tumors, with a median survival time of about 14.6 months despite maximal therapy 1 . (nature.com)
Primary endpoints were tumor response and overall survival. (thno.org)

To elucidate the role of the p53-repressive effect of ΔNp63α in radiation response, we performed a p63-siRNA knockdown experiment using human mammary epithelial cells (HMECs) expressing ΔNp63α and then performed ectopic and entopic expression experiments using human induced pluripotent stem cells (hiPSCs). (researchsquare.com)
In most cells, the centriolar component of the centrosome can function as a basal body supporting the formation of a primary cilium, a non-motile sensory organelle that monitors information from the extracellular matrix and relays stimuli into the cell via associated signaling pathways. (biomedcentral.com)
This led us to investigate if a similar relationship exists in primary cells and if the inhibition of the miRNA pathway impairs primary cilium formation. (biomedcentral.com)
Most vertebrate cells contain a single non-motile primary cilium that is assembled in a step-wise manner from the distal end of a mature centriole within the centrosome. (biomedcentral.com)
Further, defects in early stages of ciliogenesis were reported as a common feature in astrocytoma/glioblastoma cells including highly malignant T98G glioblastoma multiforme cells which expressed abnormally long centrioles and no primary cilia as evidenced by ultrastructure analysis [ 2 ]. (biomedcentral.com)
Peter Krutzik, Ph.D., a senior scientist in Gary Nolan's group at the Baxter Lab in genetics, pharmacology, microbiology, and immunology at Stanford University, described the use of phosphor flow cytometry to profile perturbations in signaling networks when peripheral blood cells are exposed to drug compounds. (genengnews.com)
Dr. Kritzik and colleagues are using phosphor-flow cytometry to study three phosphoproteins-all linked to disease and all potential drug targets-in subsets of peripheral blood cells. (genengnews.com)
Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
Adoptive transfer of expanded natural killer cells inhibited the growth of established OPM2 and high-risk primary myeloma tumors grown in the murine model. (haematologica.org)
Provides primary human cells to faculty to support research projects and NIH grant applications. (nationaljewish.org)
Seeks to conduct and enable research and NJH aimed at stem cell characterization, regeneration of the lung, and the genetic editing of stem and other primary cells. (nationaljewish.org)
Flow Cyt (intra): PD-L1 stably expressed CHO cells. (abcam.com)
Here, we show that although this receptor is present on most vascular and blood cells, the key regulatory function in tumor and wound angiogenesis is performed by β 3 integrin on bone marrow-derived cells (BMDCs) recruited to sites of neovascularization. (rupress.org)
The clinical presentation of patients with aplastic anemia includes symptoms related to the decrease in bone marrow production of hematopoietic cells (see the image below). (medscape.com)

Metabolomics assay was applied to investigate the role of C1QBP in RCC metabolism. (medsci.org)
RNA stability assay was applied to characterize the mechanism of C1QBP regulating XDH transcription. (medsci.org)

On the basis of the experiments we performed, we can conclude there is no evident difference except secreted Aβ 1-40 levels in phenotype between fAD and sAD samples. (biomedcentral.com)

Since it is technically challenging to examine the two centrosomal GW/P bodies in isolation, we investigated the potential relationship between the global population of GW/P bodies and primary ciliogenesis. (biomedcentral.com)

Aplastic anemia is a syndrome of bone marrow failure characterized by peripheral pancytopenia and marrow hypoplasia (see the image below). (medscape.com)

The effects of Pts on hepatotoxicity were measured by commercial kits, Hematoxylin and Eosin (H&E) straining, TUNEL assay, Western blot analysis, and Flow cytometry assay. (karger.com)
At CHI's recent "High-Content Flow Cytometry" meeting, Dr. Robinson talked about the ability to automate flow cytometry-based high-content screens and data processing to perform multiplexed high-content analysis in real-time, running multiple assays and samples in parallel, and to miniaturize the technology to conserve resources and reduce costs. (genengnews.com)
In addition, Dr. Robinson presented an example of multicolor flow analysis to demonstrate the ability to perform cytomics studies for pathway analysis. (genengnews.com)
The increasing use of automation and miniaturization is lowering the cost of flow cytometry-based high-content analysis in primary and secondary drug screening, making it possible to run larger screening campaigns. (genengnews.com)
We performed bioinformatics analysis to profile and identify the circRNAs mediating immune evasion in NSCLC. (biomedcentral.com)

We performed whole exome sequencing (WES) of 156 cervical cancer tissues to assess genomic features related to platinum-based chemoresistance. (biomedcentral.com)

The favorable biodistribution and high image contrast observed warrant further studies of this new reporter gene. (snmjournals.org)
A luciferase reporter assay, RNA immunoprecipitation (RIP), RNA pulldown assays and fluorescence in situ hybridization were performed to identify the interactions among circIGF2BP3, miR-328-3p, miR-3173-5p and plakophilin 3 (PKP3). (biomedcentral.com)

Through its funding of the MLPCN, NIH will support the costs of assay automation, screening and optimization chemistry. (nih.gov)

Taking advantage of the capacity for multiplexing, they can perform 14 assays per well, generating reproducible data with low intra- and inter-run variance. (genengnews.com)

Other laboratory studies can be performed on the basis of clinical judgment, depending on the nature of the infection and the organ system involved. (medscape.com)
Our focus is specialized immunologic assays relevant to immunity and inflammation and not clinical diagnostic assays. (nationaljewish.org)

The limitation of this study is that one patient cannot represent the complete heterogeneity of PeCa, especially primary tumor. (oncotarget.com)

Separate but compatible signal amplification systems enable the multiplex assay (up to two targets per assay). (wikipedia.org)

The goal of Case Studies in Environmental Medicine (CSEM) is to increase the primary care provider's knowledge of hazardous substances in the environment and to help in evaluation and treating of potentially exposed patients. (cdc.gov)

Defects in the formation and function of primary cilia underlie multiple human diseases and are hallmarks of malignancy. (biomedcentral.com)

Participants included 38 patients who underwent primary allogeneic bone marrow transplantation. (magiran.com)

The facility is a focal point for research involving microscopy and cell imaging. (nationaljewish.org)

EC are also a primary target of active CMV infection ( 16 , 17 ). (aai.org)
Although the primary site of astroviral replication seems to be the gastrointestinal tract, disseminated diseases and encephalitis have been associated with infection with classic and nonclassic astroviruses ( 11 - 16 ). (cdc.gov)

We are interested in whether this same technique can be used as a prognostic indicator for the development of therapy-related myeloid neoplasms (tMN) in patients undergoing chemotherapy and/or radiation for a primary malignant disease prior to beginning the course of treatment. (stanford.edu)

Equipment in this laboratory includes an Attune NxT Flow Cytometer from Life Technologies and a Sony SH800 Cell Sorter. (utsouthwestern.edu)

The reason why a conclusive image of the invasive process in malignant gliomas is still not available is partly due to the lack of relevant models. (nature.com)
GW/P bodies are found throughout the cytoplasm and previously published live cell imaging data suggested that in a malignant cell type (U2OS), two GW/P bodies reside at the centrosome during interphase. (biomedcentral.com)

Electrodes are connected to a secondary coil of HV resonant transformer operating at a voltage of about 2-5 kV and a frequency of about 30 kHz, with a helium flow rate of about 11 l min −1 . (nature.com)

The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)

These efforts have established that the primary cilium is a key coordinator for a variety of signaling pathways that function in development and tissue homeostasis. (biomedcentral.com)

The total number of assays to be selected depends on the scientific merit of applications and the availability of resources within the MLPCN. (nih.gov)
With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)

Ultrasound molecular imaging (UMI) is a promising tool that can provide noninvasive, non-ionizing, real-time, freehand breast cancer tumor assessment at the point of care. (stanford.edu)
However, poor UMI image quality has led researchers to trade the beneﬁts of real-time and freehand imaging for better image quality, resulting in longer exam times, higher UCA dosage, and potentially missed targets. (stanford.edu)

Two GW/P bodies as marked by GW182 and hAgo2 colocalized to the basal body of primary human astrocytes as well as human synoviocytes during interphase and specifically with the distal end of the basal body in the pericentriolar region. (biomedcentral.com)
We have previously shown that primary ciliogenesis in cultured human astrocytes and synoviocytes proceeds through stages beginning with the formation of a membrane bound vesicle at the distal end of the basal body (here referred to as stage 1) followed by the establishment and growth of a 9+0 ciliary axoneme [ 2 , 3 ]. (biomedcentral.com)
To address this question we first developed human oligonucleotide microarrays with 30.000 elements and then performed large-scale expression profiling of long-term expanded MSC and MSC during differentiation into osteoblasts. (biomedcentral.com)

The molecular mechanism of PKP3-induced PD-L1 upregulation was explored by immunoprecipitation, RIP, and ubiquitination assays. (biomedcentral.com)

Diseases1

Chemicals and Drugs1

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Phenomena and Processes2

Disciplines and Occupations1

Information Science1

Vivo11

Vitro3

Apoptosis4

Transwell2

Analysed by RT-qPCR1

Immunofluorescence2

High throughput3

Immunohistochemistry1

Viability5

ELISA1

MRNA1

Tumor cells1

Fluorescent4

Analyze2

Phenotypic2

Cytometric analysis4

Results4

Sensitivity2

Antibody2

Proteins3

Specificity1

Investigators3

Biological3

Migration1

Tissue culture1

Methods2

Survival2

Cells14

C1QBP2

Experiments1

Examine1

Peripheral1

Analysis5

Tissues1

Reporter2

Automation1

Intra1

Clinical2

Heterogeneity1

Signal1

Studies1

Hallmarks1

Allogeneic1

Microscopy1

Infection2

Chemotherapy1

Laboratory1

Malignant2

Secondary1

Defects1

Development1

Applications2

Real-time2

Human3

Mechanism1