Primary human cardiomyocyte cell substrate cardiomyocytes. Medical search. Frequent questions

Kussauer S, David R, Lemcke H ( 2019 ) hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us. (multichannelsystems.com)
In the area of cellular mechanics, Professor Saif's projects involve neurons, cancer and cardiac cells, and interactions between cells in clusters. (illinois.edu)
StemoniX ® microHeart ® platform provides a structured environment for human induced pluripotent stem cell (iPSC) derived cardiac cells to passively align into a physiologically relevant structure to closely represent human heart tissue. (stratech.co.uk)
Cardiac cells on a structured microHeart plate resemble heart tissue alignment while standard flat cultureware results in a random arrangement. (stratech.co.uk)
microHeart Assay Ready plate products are pre-plated with StemoniX human iPSC derived cardiac cells providing structured cell organization and accelerated features of maturity, delivered in ambient conditions and ready for assay use to simplify your workflow. (stratech.co.uk)

Tahrir FG, Gordon J, Feldman AM, Cheung J, Khalili K, Mohseni Ahooyi T ( 2019 ) Evidence for the impact of BAG3 on electrophysiological activity of primary culture of neonatal cardiomyocytes. (multichannelsystems.com)
The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)

The epsilon isoform of PKC is abundantly expressed in adult cardiomyocytes, being the most highly expressed of all novel isoforms, PKC-δ, -ζ, and -η. (wikipedia.org)
Since adult cardiomyocytes in humans rarely divide, the number of dysfunctional mitochondria cannot easily be diluted through cell division. (bvsalud.org)
Laminin-211 has also been used for the culture of adult human cardiomyocytes (Kuroda, 2015). (biolamina.com)
Laminin-521 also has properties that improve the isolation and expansion of adult stem cells and progenitors. (biolamina.com)
Cardiovascular diseases often cause substantial heart damage and even heart failure due to the limited regenerative capacity of adult cardiomyocytes. (thno.org)
Cardioprotective Effect of Ranolazine in the Process of Ischemia-reperfusion in Adult Rat Cardiomyocytes [J].2016,(1). (wanfangdata.com.cn)

Nuclear enriched extracts (30 µg lysate) of iPSC (Lane 1), iPSC (differentiated to cardiomyocytes) (Lane 2), FaDu (Lane 3), HeLa (Lane 4), HCT 116 (Lane 5), SK-O-V3 (Lane 6), Mouse Brain (Lane 7), Mouse Skeletal Muscle (Lane 8) were electrophoresed us. (thermofisher.com)
The same researchers later published another article with Shinya Yamanaka as co-author, where they again used laminin-211 for cardiac differentiation of human iPSC (Hirata, 2014). (biolamina.com)
Dr. Joseph Wu´s lab also showed that laminin-521 is an optimal matrix for chemically defined differentiation of human iPSC to cardiomyocytes (Burridge, 2014). (biolamina.com)
Biolaminin 521 is an optimal matrix for chemically defined differentiation of human iPSC to cardiomyocytes. (biolamina.com)

The proximal events of TLR-mediated intracellular signaling are initiated by interactions with cytosolic adapters, mainly myeloid differentiation primary response protein 88 (MyD88) [ 25 ]. (hindawi.com)
During stem cell cardiac differentiation, a distinct swish in the expression profile of laminin subunits has been reported with laminin-411/421 pre-dominantly expressed early in progenitors and laminin-211/221 expressed later in cardiomyocytes (Ja, 2015). (biolamina.com)
A highly reproducible, chemically defined, xeno-free laminin-based differentiation protocol to generate stem cell-derived cardiovascular progenitors (CVPs) was reported in 2019 (Yap, 2019). (biolamina.com)
Laminin-221 (LN221) was identified as the most likely expressed cardiac laminin and the authors show that this protein promotes differentiation of pluripotent human embryonic stem cells (hESCs) toward cardiomyocyte lineage and downregulates pluripotency and teratoma-associated genes. (biolamina.com)
This in accordance with recent data presented by researchers at Icagen Inc., demonstrating that the laminin-521 cell culture matrix maintains the differentiation potential of mouse and human satellite cell-derived myoblasts, even during long-term culture expansion (Penton, 2016). (biolamina.com)
show that laminin-521 supports more consistent and reliable differentiation over long-term culture and is the only substrate facilitating high-level fusion following long-term culture. (biolamina.com)
Laminin-521 supports increased differentiation potential without altering the traditional Pax7/MyoD paradigm and the results are translational across several mouse backgrounds, human cells, and disease states. (biolamina.com)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)

Limitations in the usage of AAV in murine models include cell types that are refractory to transduction and difficulties achieving tissue-specificity. (nature.com)

Zaniar's work is focused on identifying the mechanisms of cardiac arrhythmias using several experimental systems ranging from genetically engineered animal models to human pluripotent stem cell derived cardiac cell types. (stanford.edu)
Amend N, Thiermann H, Worek F, Wille T ( 2019 ) The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM). Toxicol Lett 308:1-6. (multichannelsystems.com)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
An immunologically identical cardiac tissue can be produced by direct reprogramming from the patients' tissue without the potential risk of tumorigenesis that occurs with induced pluripotent stem cells [ 2 ] and the ethical issue using embryonic stem cells. (thno.org)

Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
Nano-thin and nano-porous membranes in the culture system facilitated interactions between fibroblasts and cardiomyocytes in coculture. (thno.org)
The cardiac-mimetic culture system may serve as an effective tool for producing a feasible number of reprogrammed cardiomyocytes from fibroblasts. (thno.org)

MYH6:mCherry knockin human embryonic stem cell (hESC) reporter line, which allows the identification and purification of SAN-like cells. (stanford.edu)

In cardiac muscle cells, PKCε regulates muscle contraction through its actions at sarcomeric proteins, and PKCε modulates cardiac cell metabolism through its actions at mitochondria. (wikipedia.org)
As the primary source of energy in cardiomyocytes, mitochondria are the central organelle for maintaining cardiac function. (bvsalud.org)
Mitophagy is active in cardiomyocytes at baseline and in response to stress, and plays an essential role in maintaining the quality of mitochondria in cardiomyocytes. (bvsalud.org)

Western blot was performed using Anti-ATF2 Recombinant Rabbit Monoclonal Antibody (SZ17-01) (Product # MA5-32022) and 60kDa and 40kDa bands corresponding to Cyclic AMP-dependent transcription factor ATF-2 was observed across all the tested cell lines and tissues, except Mouse Skeletal Muscle. (thermofisher.com)

Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)

Superior specificity is achieved by combining Cre-mediated AAVR overexpression with whole-body knockout of endogenous Aavr , which is demonstrated in heart cardiomyocytes, liver hepatocytes and cholinergic neurons. (nature.com)

Inflammatory signaling in cardiomyocytes usually occurs as an early response to myocardial injury and entails cytosolic and mainly mitochondrial reactive oxygen species (ROS) overproduction [ 10 , 11 ]. (hindawi.com)

We and others have reported that myocardial inflammation develops in human patients and experimental models of type 1 (T1DM) and type 2 (T2DM) diabetes mellitus [ 8 , 14 , 15 ]. (hindawi.com)
These CVPs were transplanted into myocardial infarction mice, where heart function was improved as measured by echocardiogram and human heart muscle bundle formation was identified histologically. (biolamina.com)
For this purpose, a cellular model of senescent myocardial cells was set up and evaluated using colorimetric, fluorimetric, and immunometric techniques. (hindawi.com)
These results suggested that naringenin has antiaging effects on myocardial cells. (hindawi.com)
Because aging has been demonstrated to be directly related to the occurrence of cardiac disorders, together, the data have prompted us to investigate the effects of Nar in a cellular model of aged myocardial cells. (hindawi.com)
The results demonstrated that Nar exerts effective antiaging properties in myocardial cells. (hindawi.com)
The impact of ischemia-reperfusion injury on the effectiveness of primary angioplasty in ST-segment elevation myocardial infarction. (wanfangdata.com.cn)

HGF is produced and secreted by adjacent stromal and mesenchymal cells, it contributes to the development of epithelial organs in a paracrine fashion, exerts regenerative effects on epithelia in the liver, kidney, lung, and other tissues, and promotes the regression of fibrosis in numerous organs ( 7 , 8 ). (spandidos-publications.com)

HGF was cloned as a growth factor for hepatocytes ( 1 , 2 ), is identical to scatter factor (SF) and was originally discovered as a fibroblast-derived cell motility factor for epithelial cells ( 3 ). (spandidos-publications.com)

TLRs are membrane-anchored proteins present in several cell types ranging from macrophages and T and B cells to nonimmune cells such as cardiomyocytes [ 20 , 21 ]. (hindawi.com)
A Disintegrin and Metalloproteases (ADAM), originally named metalloproteinases disintegrin cystein-rich (MDC), are membrane-anchored cell surface proteins containing both disintegrin and metalloproteinase domains. (frontiersin.org)

Although the conventional activities of p53 such as cell cycle arrest, senescence, and apoptosis are well accepted as the major checkpoints in stress responses, accumulating evidence implicates the importance of other tumor suppression mechanisms. (nature.com)
To date, various mechanisms have been suggested to explain the powerful tumor-suppressive effect of p53, including the induction of cell cycle arrest, senescence, and apoptosis. (nature.com)
Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)

However it remains unknown which endocytic pathways are active in brain endothelial cells. (helsinki.fi)
In present study we characterized human brain endothelial cells, hCMEC/D3, which are widely utilized as BBB in vitro model. (helsinki.fi)
As brain endothelial cells are polarized in vivo, the aim of the study was to demonstrate the cell polarization of hCMEC/D3 cells and to study the activity and functionality of different endocytic pathways as a function of cell polarization. (helsinki.fi)

Human PRKCE gene (Ensembl ID: ENSG00000171132) encodes the protein PKCε (Uniprot ID: Q02156), which is 737 amino acids in length with a molecular weight of 83.7 kDa. (wikipedia.org)
specifically, the C2 domain of PKCε at amino acids 14-21 (also known as εV1-2) binds (RACK/RACK2), and peptide inhibitors targeting εV1-2 inhibit PKCε translocation and function in cardiomyocytes, while peptide agonists augment translocation. (wikipedia.org)
Different amino acid transporters at cell membranes work together to facilitate the movement of amino acids into and out of the brain. (edu.au)

The PKC family of serine-threonine kinases contains thirteen PKC isoforms, and each isoform can be distinguished by differences in primary structure, gene expression, subcellular localization, and modes of activation. (wikipedia.org)

In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)

Protein kinase C epsilon type (PKCε) is an enzyme that in humans is encoded by the PRKCE gene. (wikipedia.org)
Prolyl oligopeptidase (PREP) is a peptidase enzyme that has several substrates. (helsinki.fi)

The derived hESC-SAN cells display molecular and electrophysiological characteristics of bona fide nodal cells, which allowed exploration of their transcriptional profile at single-cell level. (stanford.edu)

Conditional Met KO mice were generated using Cre‑loxP methodology and characterization of these mice indicated that the HGF‑Met signaling pathway is essential in regeneration, protection, and homeostasis in various tissue types and cells. (spandidos-publications.com)

We hypothesized that AAV transgene expression could be targeted to specific tissues or cell types by selectively overexpressing AAVR. (nature.com)
The structure and structure-based modulation of ADAM17 are also described for better understanding of the various ADAM17 regulatory pathways in different cell types or tissues. (frontiersin.org)

The human SAN is poorly understood due to limited primary tissue access and limitations in robust in vitro derivation methods. (stanford.edu)
An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
This research often requires the tissue-specific expression of transgenes to better mirror human diseases, to visualize pathways being studied or to modulate the expression of a disease-related gene. (nature.com)
microHeart cardiomyocytes contract mostly along one single direction, more closely resembling the physiological contractile behavior of human heart tissue. (stratech.co.uk)

Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
The development of transgenic mouse models that express genes of interest in specific cell types has transformed our understanding of basic biology and disease. (nature.com)
Cardiomyocytes plated on microHeart express increased levels of key cardiac physiology genes compared to standard flat cultureware. (stratech.co.uk)

DCM entails the damage of the myocardium through fibrosis, steatosis, apoptosis, and hypertrophy [ 3 ] and results from the switch of substrate supply to free fatty acids (FFA) that follows the reduced levels of insulin, glucose transporters, and glucose consumption [ 4 , 5 ]. (hindawi.com)

Once activated, p53 coordinates multiple downstream pathways, thereby maintaining the homeostasis of the host cell or organism (if the stress is mild, transient, and repairable) or eliminating damaged cells (if the stress is acute, prolonged, and difficult to resist). (nature.com)
To characterize hCMEC/D3 cells for the presence of specific endocytic pathways, proteins involved into each pathway were selected. (helsinki.fi)

Human embryonic kidney cells 293 (HEK-293) and hPREP knockout cell line created from them by using CRISPR/Cas9-silencing were used in the experiments. (helsinki.fi)

ADAM17 is a metalloprotease and disintegrin that lodges in the plasmatic membrane of several cell types and is able to cleave a wide variety of cell surface proteins. (frontiersin.org)
It is estimated that as much as 10% of the cell surface proteins undergo ectodomain shedding. (frontiersin.org)
Since rate of autophagy in cells was not detected, it is impossible to determine, if there were changes in autophagy that were not reflected as changes in colocalization of these two proteins. (helsinki.fi)

Diabetic cardiomyopathy is defined as a ventricular dysfunction initiated by alterations in cardiac energy substrates in the absence of coronary artery disease and hypertension. (hindawi.com)
Is p53-dependent ferroptosis sufficient for tumor suppression in the absence of cell cycle arrest, senescence, and apoptosis? (nature.com)

Macrophage stimulating protein (MSP) was discovered as a serum protein that promoted mouse macrophage motility ( 13 ), and was later purified to homogeneity from human plasma ( 14 ). (spandidos-publications.com)
Some studies have suggested a role for this protein in maintaining centriole-nucleated sensory organelles termed primary cilia, and AS is now considered to belong to the growing class of human genetic disorders linked to ciliary dysfunction (ciliopathies). (springer.com)

The sinoatrial node (SAN) is the primary pacemaker of the heart. (stanford.edu)
Nano-thin and nano-porous membranes and heart like electric stimulus were used in the cardiac-mimetic cell-culture system. (thno.org)

Because aging has been demonstrated to be directly related to the occurrence of cardiac disorders, in the present study, the ability of naringenin to prevent cardiac cell senescence was investigated. (hindawi.com)
Relevant cellular senescence markers, such as X-gal staining, cell cycle regulator levels, and the percentage of cell cycle-arrested cells, were found to be reduced in the presence of naringenin. (hindawi.com)
To the best of our knowledge, the effect of Nar in cardiac cell senescence has not yet been studied. (hindawi.com)
Various cellular senescence hallmarks (the percentage of X-gal staining cells, the mRNA levels of the p16 and p21 cell cycle regulators, and the percentage of cell cycle-arrested cells) were investigated. (hindawi.com)

MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells. (stanford.edu)
Using this line, we performed several rounds of chemical screens and developed an efficient strategy to generate and purify hESC-derived SAN-like cells (hESC-SAN). (stanford.edu)
Single-cell RNA sequencing of CVPs derived from hESC lines identified three main progenitor subpopulations. (biolamina.com)

It is essential for cell homeostasis and survival and has been related to various diseases and pathophysiology. (helsinki.fi)

Here we generate a robust system for in vivo transgene delivery by combining the versatility of AAV-mediated transgene delivery with the specificity of cell-type-specific Cre-mediated recombination. (nature.com)

The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. (justia.com)
In any case, the respective cell types may then be selectively cultured to enrich their percentage population to eventually obtain a single cell type and culture. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
They were dissolved (10 −2 M) in DMSO and further diluted in cell culture medium. (hindawi.com)

Kraushaar U, Guenther E ( 2019 ) Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays. (multichannelsystems.com)
Oleaga C, Jalilvand G, Legters G, Martin C, Ekman G, McAleer CW, Long CJ, Hickman JJ ( 2019 ) A human in vitro platform for the evaluation of pharmacology strategies in cardiac ischemia. (multichannelsystems.com)

Reperfusion injury in humans: a review of clinical trials on reperfusion injury inhibitory strategies. (wanfangdata.com.cn)

The enhanced cell maturity results in functionality and responses which can improve assay outcomes and reduce assay cost. (stratech.co.uk)

Cell Calcium 97:102417. (multichannelsystems.com)
High throughput calcium flux analysis indicates faster and more efficient calcium handling of human cardiomycoytes in microHeart. (stratech.co.uk)

In sum, our dual reporter system facilitated an effective strategy for deriving human SAN-like cells, which can potentially be used for future disease modeling and drug discovery. (stanford.edu)

Cardiomyocytes plated on microHeart align and organize with the corresponding plate substrate topography compared to lack of cell organization in standard flat cultureware. (stratech.co.uk)
microHeart Cell Ready plate products are ready for plating with your cells allowing for structured cell organization and accelerated features of maturity. (stratech.co.uk)

We also discuss the possibility of targeting p53-mediated ferroptotic responses for the treatment of human cancers and potentially, other diseases. (nature.com)

We demonstrate that transgenic AAVR overexpression greatly increases the efficiency of transduction of many diverse cell types, including muscle stem cells, which are normally refractory to AAV transduction. (nature.com)

however it is unknown whether PKCε binds sarcomeric actin in muscle cells. (wikipedia.org)
With the use of cardiac muscle-specific laminin-211, human iPSCs were effectively differentiated into cardiomyocytes using small molecules (Minami, 2012). (biolamina.com)

Receptors for activated C-kinase (RACK) have been found to anchor active PKC in close proximity to substrates. (wikipedia.org)
Two experiments were performed on regular HEK-cells: inhibitor experiment with KYP-2047 (1 or 10 µM) and overexpression experiment (transfection with either active or inactive hPREP plasmid). (helsinki.fi)

Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. (wikipedia.org)
The level of cell polarization in cells grown on transwell permeable supports was characterized at multiple timepoints with four different methods: transepithelial electrical resistance (TEER) measurement, lucifer yellow permeability assay, alkaline phosphatase expression and ZO-1 expression. (helsinki.fi)

The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
microHeart Cell Ready and Assay Ready products provide an ideal model for cardiotoxicity and cardiac safety studies, high throughput screening and drug discovery. (stratech.co.uk)

The initiation and development of tumor cell is accompanied by elevated stresses, such as oncogene activation, DNA damage, genome instability, and reprogrammed tumor metabolism. (nature.com)

Among these unconventional activities, an iron-dependent form of non-apoptotic cell death, termed ferroptosis, attracts great interest. (nature.com)

Anatomy14

Organisms8

Diseases13

Chemicals and Drugs26

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes34

Information Science3

Cardiac Cells5

Neonatal3

Adult6

IPSC4

Differentiation20

Murine1

Pluripotent4

Fibroblasts3

Embryonic stem1

Mitochondria3

Antibody1

Functional1

Neurons1

Cytosolic1

Myocardial7

Regenerative1

Epithelial cells1

Membrane-anchored2

Mechanisms3

Endothelial cells3

Amino3

Isoform1

Cytokines1

Enzyme2

Electrophysiological1

Mice1

Tissues2

Tissue4

Genes3

Hypertrophy1

Pathways2

Knockout1

Proteins3

Absence2

Protein2

Heart2

Senescence4

HESC3

Homeostasis1

Vivo1

Culture5

20192

Clinical Trials1

Results1

Calcium2

Effective1

Organization2

Responses1

Types1

Muscle2

Active2

Expression2

Screening2

Development1

Death1