AirwayDifferentiated bronchial epithelial cellsVitroTracheal epithelial cellsHumanEpitheliumLungNHBEInflammatoryFibroblastsDifferentiationSubmucosalBiopsiesPulmonaryProgenitor cellsAsthmaDifferentially expressedExposureGrownChemokinesResponseStudiesCultureViralProteinExpressionProductionMechanismsGrowthPreviousResearch
Airway29
- The primary barriers for invading respiratory pathogens are the airway epithelial cells of the respiratory tract and antimicrobial peptides produced by these cells. (cdc.gov)
- In this study, we utilized primary normal human bronchial epithelial (NHBE) cells that are cultured at an air-liquid interface (ALI) to mimic the in vivo airway characteristics 30 . (nature.com)
- Inhaled fluticasone propionate (FP) is widely used to reduce pulmonary inflammation in chronic obstructive pulmonary disease, but the potential effects of FP on airway epithelial cells from patients with cystic fibrosis (CF) are unknown. (ersjournals.com)
- In CF disease, a nonregulated inflammatory lung response occurs through exaggerated nuclear factor (NF)-κB activation and elevated pro-inflammatory cytokines production by airway epithelial cells. (ersjournals.com)
- Cystic fibrosis (CF) is a lethal inherited disorder caused by mutations in the gene encoding the CF transmembrane conductance regulator (CFTR) in airway epithelial cells 1 . (ersjournals.com)
- One of the major clinical manifestations of CFTR mutations is neutrophil dominated inflammation 2 , 3 , implying that defective CFTR affects the immune function of airway epithelial cells, in particular the production of nuclear factor (NF)-κB-dependent chemokines. (ersjournals.com)
- During embryonic stages of lung organogenesis, TGF-β1 is pivotal in epithelial-mesenchymal interaction, during alveolarization and for airway branching morphology. (springer.com)
- Lung epithelial cells form a barrier that lines airway and alveolar surfaces and constitute an air-liquid interface. (springer.com)
- Dimethylarginine dimethylaminohydrolase (DDAH) overexpression enhances wound repair in airway epithelial cells exposed to agricultur al organic dust. (cdc.gov)
- Previous work shows that activating cAMP-dependent protein kinase (PKA) enhances airway epithelial wound repair while activating protein kinase C (PKC) inhibits wound repair. (cdc.gov)
- We hypothesized that blocking an endogenous NO inhibitor, asymmetric dimethylarginine, by overexpressing dimethylarginine dimethylaminohydrolase mitigates the effects of hog dust extract on airway epithelial would repair. (cdc.gov)
- Harnessing primary human bronchial epithelial cell culture for studies of airway epithelial injury and regeneration. (uni-muenchen.de)
- Recent progress isolating lung epithelial progenitor and stromal cells, as well as defining stem cell niche factors that are important for lung development, has led to the establishment of an in vitro three-dimensional (3D) culture system: lung organoids 2 , also known as airway organoids or pulmonary organoids. (stemcell.com)
- The human airway, from the nasal passage to the alveolar sacs, is covered with a continuous epithelial sheet that differs in morphology and cellular composition between the conducting and respiratory zones. (stemcell.com)
- Aquaporin-5 (AQP5) is a water-specific channel located on the apical surface of airway epithelial cells. (plos.org)
- These findings indicate that AQP5-mediated regulation of microtubule dynamics modulates airway epithelial barrier properties and epithelial function. (plos.org)
- Aquaporin-5 (AQP5) is a water-specific channel located on the apical membrane of epithelial cells in several sites in mammals, including corneal and pancreatic epithelium, secretory cells in salivary and lacrimal glands, and airway submucosal glands, bronchial epithelium and type I pneumocytes of the respiratory tract. (plos.org)
- Airway epithelial cells can dynamically regulate paracellular permeability in response to both physiologic and pathologic stimuli [6] , [11] . (plos.org)
- AQP5 can regulate paracellular permeability in primary airway epithelial cells [6] . (plos.org)
- Dynamic changes in airway epithelial paracellular permeability allow for receptor-ligand access across the airway epithelium, and thereby regulate cell signaling [11] . (plos.org)
- In airway epithelial cells, altered AQP5 abundance is associated with changes in actin organization and in desmoplakin localization, which could contribute to the changes in paracellular permeability. (plos.org)
- Commercially available human airway epithelial cells are compatible with air-liquid interface (ALI) culture in PneumaCult-Ex™ and PneumaCult-ALI™ when they are seeded onto cell culture inserts no later than Passage 4. (cellculturedish.com)
- In addition to human airway epithelial cells, PneumaCult media also work well with ferret and mouse airway epithelial cells. (cellculturedish.com)
- Are sphere cultures of airway epithelial cells compatible with ICC analysis? (cellculturedish.com)
- Results In IPF, the bronchiolised and enlarged distal airspaces stained for SOX2 are consistent with epithelial differentiation characteristic of conducting airway epithelium. (bmj.com)
- Conclusion Expression of SOX2 and MUC5B and lack of SPDEF in atypically differentiated cells of bronchiolised distal airspaces are consistent with abnormal programming of airway epithelial cells in IPF. (bmj.com)
- The nanotube bundles are similar to the potential of nanotubes to induce genetic damage size of microtubules that form the mitotic spindle in normal lung cells, cultured primary and immor- and may be incorporated into the mitotic spindle talized human airway epithelial cells were apparatus. (cdc.gov)
- Asthma is a clinical syndrome characterized by episodic reversible airway obstruction, increased bronchial reactivity, and airway inflammation. (medscape.com)
- Because alcohol blocks airway epithelial cell release of IL-6 in vitro , we hypothesized that alcohol exposure would alter mouse lung inflammatory responses to HDE. (cdc.gov)
Differentiated bronchial epithelial cells1
- Fully differentiated bronchial epithelial cells (n=4 replicates) were treated with 500 µg/ml (19.80 nanomoles) benzo[a]pyrene (BAP), 10 µg/ml (0.35 nanomoles) dibenzo[def,p]chrysene (DBC) or acetone vehicle control for 48 hrs and isolated mRNA were sequenced on Illumina HiSeq 3000. (nih.gov)
Vitro7
- In vitro models of RV infection of BEAS-2B and primary normal human bronchial epithelial (NHBE) cells were used. (nih.gov)
- Results: Wound closure in epithelial cells from WT mice occurred within 24 h in vitro. (cdc.gov)
- Validation and application of human in vitro models for investigating bronchial response to cigarette smoke. (uni-muenchen.de)
- Recent advances in isolating epithelial progenitor cells and stromal cells, as well as defining niche factors important for lung development, have led to the establishment of an in vitro 3D lung culture system: pulmonary (lung) organoids. (stemcell.com)
- Primary Human bronchial epithelial cells when grown in vitro have a limited lifespan and begin to deviate both in phenotype and morphology, losing the plasticity required around passage 4 or 5, for air-liquid interface (ALI) differentiation. (cancertools.org)
- The combination of VX-121/tezacaftor/VX-561 was first identified as having potential for increased efficacy based on its ability to drive higher levels of chloride transport compared to TRIKAFTA ® (elexacaftor/tezacaftor/ivacaftor and ivacaftor) in human bronchial epithelial cells in vitro . (vrtx.com)
- For in vitro studies, human bronchial epithelial cells (Beas2b) were treated with waterpipe smoke extract (WPSE, 5%), nicotine (5mM), and/or cysteamine (250muM, an autophagy inducer and anti-oxidant drug), for 6hrs. (who.int)
Tracheal epithelial cells1
- Materials/methods: We cultured primary tracheal epithelial cells in monolayers from both wild-type (WT) and dimethylarginine dimethylaminohydrolase overexpressing C57Bl/6 (DDAH1 transgenic) mice and measured wound repair using the electric cell impedance sensing system. (cdc.gov)
Human27
- Significant downregulation of β-defensin1 gene ( DEFB1 ) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
- Primary human bronchial epithelial cells HBEp cells. (cdc.gov)
- Using RNA-Seq, we identified a total of 163 and 568 differentially expressed genes in primary normal human bronchial epithelial (NHBE) cells that were exposed to diacetyl and 2,3-pentanedione, respectively. (nature.com)
- Methods: Primary human bronchial epithelial (HBE) cells cultured at air-liquid interface were treated with exogenous TGF-β1, TGF-β2, and/or a Th2 cytokine, interleukin (IL)-13. (manchester.ac.uk)
- To determine whether FP reduces cytokine production in bronchial epithelial cells via NF-κB, the authors investigated the nonstimulated and the Pseudomonas aeruginosa lipopolysaccharide (LPS) stimulated production of NF-κB-dependent interleukin (IL)-6, IL-8 and RANTES (regulated on activation, T-cell expressed and secreted) along with the activation of NF-κB in non-CF and CF human bronchial gland epithelial cells. (ersjournals.com)
- The present authors and previous studies, have previously demonstrated that epithelial expression of interleukin (IL)-8 is excessively elevated in the bronchial epithelial cells of human CF adults 6 . (ersjournals.com)
- Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research Journal of Allergy. (nottingham.ac.uk)
- Herein we elaborated effects of TGF-β1 on TJs of primary human bronchial epithelial cells. (springer.com)
- Normal human bronchial epithelial (NHBE) cell cultures have been an effective model to assess the viral-host interaction. (aacc.org)
- We are looking for a highly motivated Scientist / Senior Research Associate to help set-up and maintain ReCode's primary human bronchial epithelial. (biospace.com)
- In this study, we assess potential differences in mechanism of action for two PAHs, benzo[a]pyrene (BAP) and dibenzo-[def,p]chrysene (DBC), in a primary human 3D bronchial epithelial culture (HBEC) model based on short-term biosignatures identified from global transcriptional profiling. (nih.gov)
- While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
- For COPD, a number of disease models have been developed using primary cells, including human bronchial epithelial cells (HBECs) and pediatric asthmatic bronchial cells. (sens.org)
- The present study used isolates of the SARS-CoV-2 Delta variant and Omicron BA.1 and BA.5 sub-variants to induce an interferon response in primary human bronchial epithelial (HBE) cells grown in air-liquid interface (ALI) cultures and primary human monocytes. (news-medical.net)
- There are several categories of human disease models to study respiratory diseases and drug development: cell lines, primary cells, 3D cell models, cell co-cultures, explants and in silico models [ 4 ]. (karger.com)
- View cart "Human Lobar Bronchial Epithelial Cells, Primary" has been added to your cart. (lifelinecelltech.com)
- Lifeline ® Primary Normal Human Bronchial Tracheal Smooth Muscle Cells (HBTSMC), when grown in Lifeline® VascuLife ® SMC Medium, provide an ideal low serum (5%) culture model for the study of pulmonary biology. (lifelinecelltech.com)
- Human Bronchial/Tracheal Smooth Muscle Cells have been isolated from human bronchi and trachea and cryopreserved as primary cells. (lifelinecelltech.com)
- In silico predicted target genes were confirmed in reporter assays and in house-dust-mite (HDM) induced AAI and primary human bronchial epithelial cells (NHBE) cultured at the air-liquid interface. (edu.au)
- Here, respiratory toxicity of BP-QDs has been validated in mice and human bronchial epithelial cells. (medworm.com)
- Bronchial epithelial cells (BEAS-2B cells and primary bronchial epithelial cells) were exposed to heat-inactivated Haemophilus influenzae , Pseudomonas aeruginosa or Streptococcus pneumoniae and subsequently infected with respiratory syncytial virus (RSV), type 2 human adenovirus or influenza B. Levels of pro-inflammatory cytokines, viral replication and expression of pattern recognition receptors were determined in culture supernatants and/or cell lysates. (biomedcentral.com)
- After digestion with pronase, human epithelial cells are cultured on flask and then on inserts to recapitulate a fully differentiated epithelium. (re-place.be)
- These Human bronchial epithelial cells expressing BMI-1 retain both viability and differentiation potential of wild-type human bronchial epithelium while importantly not demonstrating changes in cell karyotype. (cancertools.org)
- Normal human bronchial epithelial cells were grown in a growth factor-supplemented medium and differentiated at ALI in bronchial epithelial differentiation medium. (cancertools.org)
- A confluent monolayer of polarized primary human bronchial epithelial cells. (cshlpress.org)
- Lysophosphatidic acid induces interleukin-13 (IL-13) receptor alpha2 expression and inhibits IL-13 signaling in primary human bronchial epithelial cells. (uchicago.edu)
- PneumaCult media also work well with primary human nasal and bronchial epithelial biopsies. (cellculturedish.com)
Epithelium4
- The alveolar epithelium is lined with type I and II alveolar epithelial cells (AECs). (stemcell.com)
- By comparison, with normal subjects, EGF immunoreactivity was significantly increased in the epithelium of bronchitic subjects and submucosa of asthmatics, and, GM-CSF immunoreactivity was increased in both epithelial and submucosal cells of asthmatics and to a lesser extent in submucosa of bronchitics. (atsjournals.org)
- Finally, we propose that Creb1/Crtc1-3 and Sec14l3 could be important for early responses of the bronchial epithelium to Th2-stimuli. (edu.au)
- The bronchial mucosa is made of pseudostratified ciliated columnar epithelium with goblet cells and basal cells. (medscape.com)
Lung7
- Viral antigens in the lung were abundant in logic, immunohistochemical (IHC), electron microscopy bronchial epithelial cells, macrophages, and fibroblasts. (cdc.gov)
- Chest radiograph of a newborn with primary pulmonary hypoplasia of the right lung showing shift of the mediastinum to the right hemithorax. (medscape.com)
- It is believed that TGF-β1 signalling is involved in controlling epithelial cell proliferation, to drive epithelial-mesenchymal transition (EMT) and it plays a major role in lung fibrosis and emphysema. (springer.com)
- The lung is a complex organ composed of many types of epithelial cells, immune cells, endothelial cells and stromal cells. (stemcell.com)
- We here investigated if pre-exposure to bacteria alters the response of lung epithelial cells to subsequent viral infection. (biomedcentral.com)
- Activation of abnormal respiratory epithelial differentiation programs may contribute to the expression of MUC5B and bronchiolisation of the distal lung, a salient feature of IPF. (bmj.com)
- This pulmonary exposure to alcohol can damage the bronchial epithelial layer of the lung [ 6 , 7 ], while impairing the lung's ability to repair epithelial wounding via inhibition of the PKA pathway [ 8 ]. (cdc.gov)
NHBE2
- Using RNA-Seq, we performed global transcriptomic profiling in primary NHBE cells exposed to diacetyl or 2,3-pentanedione. (nature.com)
- Our data showed that diacetyl and 2,3-pentanedione induced significant transcriptomic changes, including those related to ciliogenesis, in the primary NHBE cells. (nature.com)
Inflammatory3
- It was shown by the authors that fluticasone propionate exerts an anti-inflammatory effect by blocking a signal transduction leading to a reduced level of IκB-α/β kinases in bronchial epithelial cells. (ersjournals.com)
- The effects of corticosteroids are predominantly mediated by the inhibition of NF-κB deoxyribonucleic acid (DNA)-binding activity, which controls genes encoding inflammatory cytokines, chemokines and adhesion molecules in respiratory epithelial cells 10 - 13 . (ersjournals.com)
- Evidence that long-term exposure to atmospheric particulates decreases the body's defences against bacterial and viral pathogens appear sufficient, particularly by altering the response of bronchial epithelial cells via inflammatory processes and endothelial dysfunction, and exacerbating the conditions of chronically ill patients, particularly those with COPD and asthma [8-14], and children with acute respiratory infections [15]. (bmj.com)
Fibroblasts1
- TGF-β can be generated by many cells, such as macrophages, epithelial cells, fibroblasts, and eosinophils ( 9-11 ). (atsjournals.org)
Differentiation1
- Compared to primary cells, stem cells are more versatile for differentiation, are often immortalized, and do not require direct isolation from tissue. (sens.org)
Submucosal2
- A significant correlation was found between the number of epithelial or submucosal cells expressing TGF- β in both asthma and chronic bronchitis and basement membrane thickness and fibroblast number. (atsjournals.org)
- NRG1α was detected in serous cells within normal submucosal glands and in epithelial cells lining honeycombing areas in IPF, and was not detected in other patients. (bmj.com)
Biopsies1
- its expression was compared with that of epidermal growth factor (EGF) and granulocyte macrophage colony-stimulating factor (GM-CSF) in bronchial mucosal biopsies from 13 normal subjects, 24 asthmatics, and 19 patients with chronic bronchitis. (atsjournals.org)
Pulmonary1
- Pulmonary hypoplasia (PH) may be primary or secondary. (medscape.com)
Progenitor cells1
- We have developed a protocol for culturing primary neuron progenitor cells (NPCs) derived from mouse embryos at embryonic day 15 (E15). (re-place.be)
Asthma1
- We conclude that the observed effects contribute to loss of epithelial integrity during atopic asthma. (springer.com)
Differentially expressed1
- In this study, we aimed to assess host differentially expressed gene signatures in respiratory tract epithelial cells after influenza A virus pdmH1N1 or H7N9 infection. (aacc.org)
Exposure1
- Pre-exposure of epithelial cells to bacteria alters the response to subsequent viral infection depending on the types of pathogen involved. (biomedcentral.com)
Grown1
- Bronchial/Tracheal Smooth Muscle Cells can be grown without phenol red or antimicrobials when cultured in VascuLife SMC Medium. (lifelinecelltech.com)
Chemokines2
- BUD and FORM suppress RV-induced chemokines and growth factors in bronchial epithelial cells in a concentration-dependent, synergistic or additive manner. (nih.gov)
- Increasing evidence suggests that respiratory epithelial cells with dysfunctional CFTR have exaggerated activation of NF-κB/inhibitor of NF-κB complex associated with upregulated expression of chemokines 4 , 5 . (ersjournals.com)
Response1
- IL-33 is predominantly produced by epithelial cells in response to protease containing aeroallergens and its release is mediated by dual oxidase 1 (DUOX1). (justia.com)
Studies2
- By comparing with previous studies, we speculate that primary cells generally are more sensitive to BP-QDs-induced damage than cancer cells. (medworm.com)
- Imaging studies also must be performed to detect the sites of either primary tumors or metastases. (medscape.com)
Culture1
- RÉSUMÉ Cette étude prospective a été menée dans six hôpitaux d'Edmonton (Canada) a n de déterminer les facteurs associés à l'obtention d'expectorations à des ns de mise en culture et les effets de celle-ci sur la prise en charge des patients atteints de pneumonie communautaire (PC). (who.int)
Viral1
- The virus has been isolated from skin lesions demonstrated abundant viral antigens in surface epithelial on a rope squirrel from Zaire (6). (cdc.gov)
Protein2
- Expression of polycomb protein BMI-1 maintains the plasticity of basal bronchial epithelial cells. (cancertools.org)
- However, the primary defect is thought to be a GMD-regulating protein. (medscape.com)
Expression1
- Interestingly, the expression of two IκB kinases (IKK)-α/β, the degradation of cytosolic IκB-β inhibitor and the NF-κB deoxyribonucleic acid binding activity were markedly reduced after FP treatment in both CF and non-CF bronchial epithelial cells. (ersjournals.com)
Production2
- It was demonstrated that a relevant concentration of FP (10 −8 M) inhibited constitutive and P. aeruginosa LPS-induced IL-6 and IL-8 production of non-CF and CF bronchial epithelial cells. (ersjournals.com)
- Further, blocking nitric oxide (NO) production in bronchial epithelial cells prevents PKA activation. (cdc.gov)
Mechanisms1
- Throughout, the focus is on epithelial cells and how polarity mechanisms facilitate the development of their apical and basolateral surfaces, tube formation (e.g., in blood vessel development), exchange of waste and nutrients, and the generation and maintenance of specialized structures at the cell surface (e.g., cilia). (cshlpress.org)
Growth1
- Granulocyte macrophage-colony stimulating factor (GM-CSF) is a hematopoietic growth factor over-expressed in the bronchial mucosa of asthmatics, which is involved in eosinophil and macrophage accumulation in tissues ( 14 , 15 ). (atsjournals.org)
Previous1
- As described in the previous two chapters, primary strategies to achieve this goal are to prevent youth and young adults from starting smoking and to help current smokers quit expeditiously. (nationalacademies.org)
Research1
- Your research outcomes will be more representative of the general population: Primary Bronchial/Tracheal SMCs can come from many donors. (lifelinecelltech.com)