The ActiveHOOK⢠BSA rapidly couples to free sulfhydryl groups on peptides and proteins. (gbiosciences.com)
Bovine serum a2
Current, antibody-based diagnostical methods utilize antibodies against histamine raised by conjugating histamine to a large immunogenic protein carrier, such as bovine serum albumin (BSA) or ovalbumin (OVA) via its primary amine group. (justia.com)
Bovine serum albumin is a single polypeptide of 67kDa and consists of 59 lysine residues, of which 30-35 have primary amines that can react with crosslinkers for the coupling of peptides. (gbiosciences.com)
Receptors1
Although the efficacy of adoptive transfer of tumor infiltrating lymphocytes (TILs) was examined over several decades, genetically engineered T cells expressing chimeric antigen receptors (CARs) rapidly replaced the application of TILs due to their high specificity, non-MHC-restricted recognition of tumor antigen, superior potency, and improved in vivo persistency [ 9 , 13 , 14 ]. (biomedcentral.com)
Specificity1
Syd Labs team has successfully developed a large number of high specificity and high affinity rabbit monoclonal antibodies against a wide range of difficult targets such as small molecules, antibody idiotype, and antigens non-immunogenic in murine, camel or Ilama. (sydlabs.com)
Antibodies2
Consequently, only the imidazole will be exposed to lymphocytes, which commonly results in generation of antibodies that recognize protein-bound histamine with only limited affinity and sensitivity for free histamine. (justia.com)
Syd Labs has successfully developed human and monkey monoclonal antibodies using antigen specific single B cell-based antibody generation technologies. (sydlabs.com)
Nuclear3
Studies on rodent UGT genes have demonstrated that the transcription factors hepatocyte nuclear factor 1 (HNF1) and CAAT-enhancer binding protein are important positive regulators of UGT expression in the liver (Hansen et al. (aspetjournals.org)
The viral C promoter regulates the expression of all EBV nuclear antigens (EBNAs), some of which are very far away from the C promoter. (bvsalud.org)
The mammalian genome comprises nuclear DNA (nDNA) derived from both parents and mitochondrial DNA (mtDNA) that is maternally inherited and encodes essential proteins required for oxidative phosphorylation. (regenerativemedicine.net)
After detection and isolation of antigen specific memory B cells by flow cytometry-based single B cell sorting, the antibody variable regions (VH and VL) from single or clonal B cells are cloned and sequenced. (sydlabs.com)
We will link two approved anti-tumor antibiotics to your purified monoclonal antibody using our proprietary conjugation method. (sydlabs.com)
The anti-tumor agent will remain bound to the monoclonal antibody and inactive but will be released and activated by reduction once the immunocomplex binds to the cell surface antigen and internalized. (sydlabs.com)
Reaction2
ActiveHOOK⢠carrier proteins have been pre-activated with the heterobifunctional protein cross linker sulfoSMCC and therefore are ready for the addition of cysteine containing peptides or proteins for a simple one step reaction. (gbiosciences.com)
Due to the risk of adverse or immunogenic reaction or replication connected with the more efficient viral vectors, non-viral vectors are in many cases considered as a preferred strategy for gene delivery into eukaryotic cells. (bvsalud.org)
Chemicals1
The UGT content of cells and tissues is a major determinant of our response to those chemicals that are primarily eliminated by conjugation with glucuronic acid. (aspetjournals.org)
Cells4
Along this line, nanoparticle (NP) delivery systems can enhance the immunogenicity of antigens by protecting them from degradation and increasing their uptake by antigen-presenting cells, as well as offering co-delivery with adjuvants. (univ-nantes.fr)
All the fluorinated compounds are unable to transfect RD-4 cells, if used alone, but they are all able to deliver a plasmid carrying an enhanced green fluorescent protein (EGFP) expression cassette, when co-formulated with 1,2-dioleyl-sn-glycero-3-phosphoethanolamine (DOPE) in a 1:2 ratio. (bvsalud.org)
Research endeavors over the past several decades have identified various types of cells as suitable living drugs and versatile drug carriers. (biomedcentral.com)
In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
Suitable1
However, inclusion of protein antigens fromStreptococcus pneumoniaecombined with potent adjuvants and a suitable delivery system are expected to both extend protection to serotype strains not represented in the formulation and stimulate a broader immune response, thus more effective in young children, elderly, and immunocompromised populations. (univ-nantes.fr)
Small3
Furthermore, very small molecules, such as histamine (11.14 g/mol) which consists of an imidazole ring and a short carbon chain terminated with a primary amine, pose even more of a challenge due in part to their limited functionalities. (justia.com)
The importance of histamine in various physiological functions and its involvement in allergenic responses make this small molecule one of the most studied biogenic amines. (justia.com)
Keyhole limpet hemocyanin is a widely used carrier protein due to its large molecular mass (4.5x105-1.3x107Da aggregates) and large number of primary amines that can react with cross-linkers for the coupling of peptides. (gbiosciences.com)
Response1
The use of cationized BSA is known to elicit greater immunogenic response compared to normal BSA (1-3). (gbiosciences.com)
Viral2
There are over 150 viral proteins - many of which are uncharacterized - and humoral immunity to ASFV has not been closely examined. (preprints.org)
Nonviral vectors or carriers, of the type with which the present invention is concerned, will thus have to overcome the same obstacles as a viral vector. (justia.com)
Recombinant1
Through molecular biology techniques, the pentapeptide sequence, molecular weight, and architecture of the ELP can be precisely controlled for subsequent an purified as a recombinant polypeptide, resulting in monodisperse, non-immunogenic (Urry et al. (justia.com)
Immune2
For the development of vaccines against African swine fever virus (ASFV), immunogenic antigens that generate protective immune responses need to be identified. (preprints.org)
Genetic material can also be used to provide protective immune responses in vivo by injection of DNA that encodes immunogenic proteins, i.e., ones that can stimulate the desired immune response. (justia.com)
Vivo1
Although the efficacy of adoptive transfer of tumor infiltrating lymphocytes (TILs) was examined over several decades, genetically engineered T cells expressing chimeric antigen receptors (CARs) rapidly replaced the application of TILs due to their high specificity, non-MHC-restricted recognition of tumor antigen, superior potency, and improved in vivo persistency [ 9 , 13 , 14 ]. (biomedcentral.com)
Prevents1
Genetic material has also been transferred into cells to introduce proteins that are absent due to an inherent genetic flaw in the cell that expresses an inactive protein or else prevents expression of the protein altogether. (justia.com)
Drug carriers2
Genetically engineered biopolymers show great promise as macromolecule, gene and drug carriers due to genetic control of composition and monodispersity. (justia.com)
Research endeavors over the past several decades have identified various types of cells as suitable living drugs and versatile drug carriers. (biomedcentral.com)
Fusion protein2
For instance, an ELP composition may comprise an ELP domain covalently linked to a small molecule or an ELP linked to a therapeutic polypeptide by a peptide bond (i.e. an ELP fusion protein). (justia.com)
Microvesicles loaded with MCs encoding a thymidine kinase (TK)/nitroreductase (NTR) fusion protein produced prolonged TK-NTR expression in mammary carcinoma cells. (regenerativemedicine.net)
Gene2
The UGT1 family constitutes a complex gene locus on human chromosome 2q37 and comprises 13 first exons that encode the unique N-terminal domains of the UGT1A proteins and exons 2 to 5 that encode the C-terminal domain, which is identical in all UGT1A family members ( Owens and Ritter, 1992 ). (aspetjournals.org)
In particular, the present invention relates to nonviral gene carriers comprising multifunctional molecular conjugates which include, inter alia, lipopolyamines of a particular configuration, a component which promotes endosome disruption, and a receptor specific binding component. (justia.com)
Expression1
Studies on rodent UGT genes have demonstrated that the transcription factors hepatocyte nuclear factor 1 (HNF1) and CAAT-enhancer binding protein are important positive regulators of UGT expression in the liver (Hansen et al. (aspetjournals.org)
Potential1
Our present research areas are: (1) opioid receptor subtypes and bifunctional ligands, (2) design and synthesis of highly G-protein biased opioid agonists as potential replacements for medical narcotics presently in use (3) design and synthesis of haptens for vaccine construction for treatment and prevention of drug abuse (4) design and synthesis of Toll-4 receptor antagonists (5) synthesis and pharmacology of new clandestine designer drugs. (nih.gov)
High1
The animal positive respone to the antigen is critical to isolate high specificity and high affinity rabbit monoclonal antibodies. (sydlabs.com)