Present polypyrimidine tract binding protein. Medical search. Frequent questions

Polypyrimidine tract-binding protein 1 is a protein that in humans is encoded by the PTBP1 gene. (wikipedia.org)
During neuronal differentiation, miR-124 reduces PTBP1 levels, leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. (wikipedia.org)
The RNA-binding protein, polypyrimidine tract-binding protein 1 (PTBP1), also named hnRNP I, acts as a critical mediator of insulin biosynthesis through binding to the pyrimidine-rich region in the 3'-untranslated region (UTR) of insulin mRNA. (molcells.org)
PTBP1 is present in β cells of both mouse and monkey, where its levels are increased by glucose and insulin, but not by insulin-like growth factor 1. (molcells.org)

These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. (wikipedia.org)
This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. (wikipedia.org)
Initiation of BAG-1 protein synthesis can occur by both cap-dependent and cap-independent mechanisms and it has been shown that synthesis of BAG-1S is dependent upon the presence of an internal ribosome entry segment (IRES) in the 5′-UTR of BAG-1 mRNA. (nature.com)
2002). Translation of polioviral mRNA is inhibited by cleavage of polypyrimidine tract-binding proteins executed by polioviral 3C(pro). (nature.com)
Interestingly, an alternative initiation codon has been shown to be present within the coding region of p53 mRNA. (iisc.ac.in)
In this mechanism, the ribosomes are recruited to a complex RNA structural element known as 'Internal Ribosome Entry Site (IRES)', generally present in the 5' untranslated region (UTR) of mRNA. (iisc.ac.in)
The IRES directing the translation of p53 is in the 5'-untranslated region of the mRNA, whereas the IRES mediating the translation of ΔN-p53 extends further into the protein-coding region. (iisc.ac.in)
thereby ensuring that sufficient insulin mRNA is present to serve as the substrate for rapid insulin biosynthesis under conditions of greater insulin demand. (molcells.org)
It binds the 3′ AG splice acceptor dinucleotide of the pre-mRNA target intron, and forms a heterodimer with U2AF2 , which binds the adjacent polypyrimidine tract. (massgenomics.org)
This machinery recognizes pre-mRNA sequences at several motifs - the 5′ and 3′ splice sites, the branch point, and polypyrimidine tracts - positioned at exon-intron boundaries [ 4 ]. (biomedcentral.com)
Proteins shown in red (FDR 0.01) represent the mRNA interactome. (nature.com)
c ) Overlap of mRNA interactome proteins in yeast and HuH-7. (nature.com)
d ) Validation of the yeast mRNA interactome using western blotting of input samples and eluate after interactome capture with specific antibodies (ADH1, alcohol dehydrogenase 1, PUB1) or against TAP-tagged proteins (PGK1, phosphoglycerate kinase 1, TDH1, triose phosphate dehydrogenase, TRX2, thioredoxine 2, SHE2, Swi5p-dependent HO Expression 2). (nature.com)
It is transcribed in a 14 kb mRNA, and the 11kb cDNA encodes a 3685 amino acid protein of 427 kDa called Dystrophin. (pressbooks.pub)
Phosphorylation by mTOR of 4E-BP1 disrupts its binding to eIF4E, a protein that binds the 5'-cap structure of mRNA. (biomedcentral.com)

The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. (wikipedia.org)
Chemical shift changes upon the addition of single-stranded RNAs (ssRNAs) identified a group of residues that form a positively charged patch on the protein surface as the binding site responsible for the previously reported affinity for nucleic acids. (pdfroom.com)
To address the second, we investigated RNAs bound by the metabolic enzyme hydroxysteroid dehydrogenase 17-β 10 (HSD17B10). (nature.com)
After cell lysis, polyadenylated RNAs were captured on oligo d(T) beads followed by stringent washes to remove non-crosslinked proteins. (nature.com)
Human ZAP inhibits many viruses, including HIV and coronaviruses, by binding to viral RNAs to promote their degradation and/or translation suppression. (bvsalud.org)
instead, GAPDH binds to the 3' termini of plus- and minus-strand RNAs of JEV by electrophoretic mobility shift assays. (biomedcentral.com)
Accordingly, GAPDH binds to the minus strand more efficiently than to the plus strand of JEV RNAs. (biomedcentral.com)

The hnRNP proteins have distinct nucleic acid binding properties. (wikipedia.org)
In order to obtain more detailed information regarding the interaction between host proteins and the defined viral nucleic acid and/or proteins, we investigated the host factors associating with the main replicase enzyme, NS5, in the JEV-infected cells using yeast-two hybrid screening. (biomedcentral.com)

An extensive bioinformatics analysis of the coronavirus replicase proteins by Snijder et al. (pdfroom.com)

The coronavirus replication cycle begins with the translation of the 29-kb positive-strand genomic RNA to produce two large polyprotein species (pp1a and pp1ab), which are subse- quently cleaved to produce 15 or possibly 16 nonstructural proteins (nsp's) (11). (pdfroom.com)

It may also promote the binding of U2 snRNP to pre-mRNAs. (wikipedia.org)
The large ~280-kDa U5 snRNP protein PRPF8 is central to the dynamics of spliceosome assembly [ 11 ]. (biomedcentral.com)
The spliceosomes consist of four small nuclear ribonucleoproteins (snRNPs) and over a hundred of non-snRNP proteins that associate with snRNPs at some point during the splicing [ 4 - 6 ]. (ijbs.com)
The major difference lies in the ribonucleotide components, while the major spliceosome contains U1, U2, U4, and U6 snRNPs, the minor one consists of functionally equivalent U11, U12, U4atac, and U6atac snRNPs, with U5 snRNP present in both spliceosomes [ 9 - 10 ]. (ijbs.com)

Insect genes that harbor U12-type introns belong to several functional categories among which proteins binding ions and nucleic acids are enriched and these few categories are also overrepresented among these genes that preserved minor type introns in Diptera . (ijbs.com)
Most eukaryotic protein coding genes are intervened by non-coding sequences called introns ( Int ervening r egi ons ) [ 1 ], which are being removed from the primary transcript in the process of splicing [ 2 - 3 ]. (ijbs.com)
Additionally, U12-type introns lack a polypyrimidine tract between the BPS and the 3′ splice site. (ijbs.com)
We know now that the high adaptive value of introns resides in the fact that they permitted exon shuffling in the past and alternative splicing in the present ( Sharp, 1994 ). (biologists.com)

Polypyrimidine tract-binding protein interacts with HnRNP L". FEBS Letters. (wikipedia.org)

RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
RNA-binding proteins (RBPs) regulate gene expression at the post-transcriptional level. (encyclopedia.pub)

The different interactions between viral proteins and cellular host proteins are required for efficient replication of HIV-1. (biomedcentral.com)
The integration process is catalyzed by viral protein integrase which utilizes different host proteins for integration of viral DNA [ 1 ]. (biomedcentral.com)

2006). Polypyrimidine tract binding protein regulates IRES-mediated gene expression during apoptosis. (nature.com)
Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)
They play major roles in the tissue- and stage-specific expression of protein isoforms as well as in the maintenance of protein homeostasis. (encyclopedia.pub)

The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). (wikipedia.org)
This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)

Transcriptomic analysis of ZAP-isoform-specific knockout cells revealed uncharacterized host mRNAs targeted by ZAPL/S with broad cellular functions such as unfolded protein response (UPR), epithelial-mesenchymal transition (EMT), and innate immunity. (bvsalud.org)

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a cellular metabolic protein, was found to colocalize with viral RNA-dependent RNA polymerase (NS5) in JEV-infected cells. (biomedcentral.com)

There are three major isoforms of BAG-1 in mammalian cells, termed BAG-1L (p50), BAG-1M (p46) and BAG-1S (p36) that function as pro-survival proteins and are associated with tumorigenesis and chemoresistance. (nature.com)
CRP is a dynamic protein undergoing conformational changes upon activation in inflammatory microenvironments between pentameric and monomeric isoforms. (bvsalud.org)

RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
LIN28 (LIN28A and LIN28B) proteins are highly conserved small cytoplasmic RNA-binding proteins (RBPs) that function as pluripotency factors, regulating the transition from self-renewal to a differentiated cell fate [ 1 ] . (encyclopedia.pub)

Polypyrimidine tract binding protein and associated splicing factor (PSF) has diverse functions inside the cell such as transcriptional regulation, DNA repair, acts as nucleic acids binding protein and regulate replication and infectivity of different viruses. (biomedcentral.com)

Here, we show that the BAG-1 IRES maintains synthesis of BAG-1 protein following exposure of cells to the chemotoxic drug vincristine but not to cisplatin and that this is brought about, in part, by the relocalization of PTB and PCBP1 from the nucleus to the cytoplasm. (nature.com)
One of the important mechanisms used for protein synthesis during these conditions is internal initiation. (iisc.ac.in)
In many cell lines, exposure to rapamycin results in a relatively small decrease in overall protein synthesis (~15-20%), but dose result specifically in G1 cell cycle arrest. (biomedcentral.com)

ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. (thermofisher.com)

In addition, proteasome inhibitors blocked the degradation of the incoming viral protein N, suggesting the uncoating process and genomic RNA release were suppressed. (bvsalud.org)

1996). HGF receptor associates with the anti-apoptotic protein BAG-1 and prevents cell death. (nature.com)

To develop a vaccine capable of stimulating intestinal mucosal immunity, we prepared a bacterium (Lactococcus lactis)-like particle (BLP) vaccine (S1-BLPs) displaying the S1 protein, a domain of PEDV spike protein (S), based on gram-positive enhancer matrix (GEM) particle display technology. (bvsalud.org)
In contrast to ACE2, the short dACE2 isoform lacks the ability to bind the spike protein of SARS-CoV-2. (bvsalud.org)

Gene Ontology and pathway enrichment analyses and assessment of RNA binding proteins were performed by R software, circlncRNAnet and Encyclopedia of RNA Interactomes (ENCORI). (xiahepublishing.com)
b ) Volcano plot showing the distribution of proteins according to their enrichment in crosslinked (CL) over non-CL samples. (nature.com)

1999). Cellular stress in Xenopus kidney cells enhances the phoshorylation of eukaryotic initiation factor eIF(4E) and the association of eIF4F with poly(A)-binding protein. (nature.com)

For example, a word in an open reading frame (ORF) has a direct influence on the primary amino acid sequence of a protein and hence is under strong selective pressure. (biomedcentral.com)
Until the discovery of ribozymes, it had been assumed that enzymes could only be generated with the diversity of structures possible with the amino acids in proteins. (pressbooks.pub)

We show that this conserved RNA interactome harbours many proteins without previously assigned roles in RNA biology (enigmRBPs), including surprisingly many metabolic enzymes. (nature.com)

The transcriptomic and epigenomic regulatory landscape of the MAC genome, which has 80% protein-coding genes and short intergenic regions, is poorly understood. (uni-frankfurt.de)
Mutations in cis-acting splicing elements or changes in the activity of constitutive or alternative splicing could have a profound regulatory proteins that compromise the accuracy of either impact on human pathogenesis, in particular in tumor development and progression. (biologists.com)

Further, it has been shown that polypyrimidine tract binding (PTB) protein acts as an important regulator of p53 IRES activities. (iisc.ac.in)
C-reactive protein (CRP), an active regulator of the innate immune system, has been related to COVID-19 severity. (bvsalud.org)

Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. (nature.com)
We also determined the RNA targets of an RNA-binding mitochondrial enzyme and show its specificity in RNA binding. (nature.com)
They are also present within mitochondrial and chloroplast genes. (pressbooks.pub)

Currently, there are 171 genes in SZGR 2.0 (with evidence in schizophrenia) that are present at this pathway. (uth.edu)

It specifically binds Na+/K+-ATPase -subunit to inhibit Na+/K+-ATPase, which has been explored in a number of clinical studies for cancers treatment today. (tech-strategy.org)

In the present study, we have identified a new HIV-1 integrase interacting protein, polypyrimidine tract binding protein and associated splicing factor (PSF) through pull down assay and co immunoprecipitation of IN with mammalian cell protein. (biomedcentral.com)

This binding site is similar to the ssRNA-binding site of the sterile alpha motif domain of the Saccharomyces cerevisiae Vts1p protein, although the two proteins do not share a common globular fold. (pdfroom.com)

In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. (thermofisher.com)

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)

We have shown previously that BAG-1 IRES-meditated initiation of translation requires two trans -acting factors poly (rC) binding protein 1 (PCBP1) and polypyrimidine tract binding protein (PTB) for function. (nature.com)

2 Risk factors, including viral infection, alcoholic cirrhosis and fatty liver, along with chronic inflammatory disorders of the biliary tract, genetic diseases and carcinogens, contribute to the risk of HCC. (xiahepublishing.com)
In an important model of premature aging, Werner's Syndrome, the genetic problem seems to be lack of a helicase protein that can unwind G-quadruplexes. (anti-agingfirewalls.com)

In an attempt to study the host interacting protein of IN, we have identified a new interacting host protein PSF which is a splicing factor and elucidated its role in integration and viral replication. (biomedcentral.com)
Identification of these interacting host cellular protein provide better understanding of mechanism of viral replication and subsequently development of new therapeutic approaches. (biomedcentral.com)
viral proteins may decoy some host factors for assisting viral replication. (biomedcentral.com)

A search of the protein structure database indicates that the globular domain of the NAB represents a new fold, with a parallel four-strand �-sheet holding two �-helices of three and four turns that are oriented antiparallel to the �-strands. (pdfroom.com)
The formation of G-quadruplex DNA is easily conceivable in DNA sequences that are present as single strands in cells, such as the telomeric overhang. (hindawi.com)

Numerous studies have shown that replication of RNA viruses is involved in many specific RNA-RNA, RNA-protein, and protein-protein interactions. (biomedcentral.com)

While the level of p53 protein is maintained low in unstressed conditions, there is a rapid increase in the functional p53 protein levels during stress conditions. (iisc.ac.in)
The structural and functional integrity of this overhang is based on its association with a constellation of specific proteins, some of them belonging to the shelterin complex [ 10 ]. (hindawi.com)

The probable mechanism of action of PTB protein has also been investigated. (iisc.ac.in)
Alternative splicing is a crucial mechanism for generating protein diversity. (biologists.com)

Stimulated by the pioneering studies on the cationic porphyrin TMPyP4 and the natural product telomestatin, follow-up studies have developed, rapidly leading to a large diversity of macrocyclic structures with remarkable-quadruplex binding properties and biological activities. (hindawi.com)

Different splice variants of a given protein can display different and even antagonistic biological functions. (biologists.com)

The present review mainly focused on the latest updates on RUNX2 in BCSCs and their roles in breast cancer progression and drug resistance, providing insight that may aid the development of RUNX2‑based diagnostics and treatments for breast cancer in clinical practice. (spandidos-publications.com)
CCCTC-binding factor (CTCF) is a conserved transcription factor that performs diverse roles in transcriptional regulation and chromatin architecture. (preprints.org)

The former protein allows BAG-1 IRES RNA to attain a structure that permits binding of the ribosome, while the latter protein appears to be involved in ribosome recruitment. (nature.com)

Treating SMMC-7721 cells with Rapamycin, a specific mTOR inhibitor, significantly reduce hTERT protein level but did not affect hTERT transcription. (biomedcentral.com)

Anatomy5

Organisms1

Chemicals and Drugs15

Phenomena and Processes14

Information Science3

PTBP14

MRNA15

RNAs7

Nucleic acid2

Replicase proteins1

Nonstructural proteins1

SnRNP4

Introns4

HnRNP1

Post-transcriptional2

Host proteins2

Expression3

Nuclear2

MRNAs1

Polymerase1

Isoforms2

RBPs3

Regulate1

Synthesis3

Cytoplasmic1

Degradation1

Receptor1

Spike protein2

Enrichment2

Eukaryotic1

Amino2

Enzymes1

Regulatory2

Regulator2

Mitochondrial3

Genes1

Subunit1

Mammalian1

Motif1

Associate1

Knockdown1

Initiation1

Genetic2

Viral replication3

Strands2

Interactions1

Functional2

Mechanism2

Diversity1

Biological functions1

Roles2

IRES1

Specific1