ChromatographyAgilentPurificationAnalytical HPLCDetectorChiralCompoundsPrepChromatogramsPurityFractionSeparationMethodsKNAUERDetectionTasksBiological activityHighHardwareSemiMaterialsGuardStandardSingleColumnsUHPLCAnalytical and preparativeChromatographicPumpAutosamplerYieldsLiquidPumpsSolventFractionsTemperatureReagentScaleMethodGuard columnSystemPhaseSelectorCellularAnalysisSpectralApplicationMixtureCompactFound
Chromatography12
- In the present study, cordycepin was prepared by preparative high‑performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large‑scale isolation of cordycepin in the future. (spandidos-publications.com)
- HPLC, high-performance liquid chromatography. (spandidos-publications.com)
- Preparative high performance liquid chromatography (prep-HPLC) can shorten the production cycle, has relatively low operating costs, and is simple to carry out. (spandidos-publications.com)
- In addition, an efficient method was successfully established to separate and purify the above anthraquinones by high-speed counter-current chromatography and preparative HPLC. (virginia.edu)
- The fractionation of the alkaloids was performed with medium pressure liquid chromatography (MPLC) and the fractions were analyzed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). (helsinki.fi)
- Used Gilson Preparative HPLC and Flash Chromatography System. (bidspotter.com)
- The separation of the active pharmaceutical ingredients (APIs) from their impurities can be easily achieved with a preparative chromatography system. (advion.com)
- There is then compared with semi-preparative chromatography followed by its inability to distinguish fenofibrate signals from different molecules. (medes.ru)
- Fitting to your needs and environment The Pure chromatography instruments are extremly compact, ensure safety on the highest level and are easy to use for any flash or prep HPLC application. (thomassci.com)
- With the capability to purify compounds by preparative HPLC and flash chromatography on the same instrument, Gilson's PLC 2050/2250/2500 Purification Systems simplify and streamline compound purification. (thomassci.com)
- Development of HPLC columns has began around 1980 and resulted in the development of a series of polymer-based columns which have turned into commercial products, including analysis SEC columns, preparative SEC columns, sugar and organic acid analysis columns, aqueous SEC columns, reverse-phase partition columns, and ion chromatography columns. (hitachi-hightech.com)
- The AZURA® Prep Compact system is the perfect start into preparative chromatography. (gmi-inc.com)
Agilent1
- Explore Agilent offering on HPLC and UHPLC systems and solutions, suitable for a broad range of analytical, bio-analytical, purification and other specialized workflows. (agilent.com)
Purification5
- Preparative and semi-preparative HPLC columns are available with proven chemistries for seamless scaling from analytical HPLC to gram scale purification, providing a tailored solution for even the most challenging samples. (agilent.com)
- InfinityLab Prep LC columns offer optimal purification speed, throughput and lifetime, and are precisely matched with corresponding InfinityLab analytical HPLC columns. (agilent.com)
- This application note features the purification of five active ingredients found in over-the-counter (OTC) drugs including caffeine, glafenine, ketoprofen, flavone, and fenofibrate (Figure 1), by a preparative purification workflow with confirmation using a compact mass spectrometer. (advion.com)
- With the complete, semi-preparative HPLC system you master your isocratic purification tasks. (gmi-inc.com)
- Separation and purification of bioactive botrallin and TMC-264 by a combination of HSCCC and semi-preparative HPLC from endophytic fungus Hyalodendriella sp. (bvsalud.org)
Analytical HPLC1
- carry out chemical analyses using modern instruments including analytical HPLC, LC-MS, TLC, UV/Vis and fluorescence spectrophotometry. (biotium.com)
Detector1
- High-effective preparative system containing UV-VIS DAD detector, preparative pump, and gradient box. (stakrr.com)
Chiral2
- Available in over 40 unique achiral and chiral selectivities, Axia advanced preparative column packings and column hardware designs offer several advantages. (phenomenex.com)
- Various set-ups isozid involving coupling GC, HPLC and in preparative chiral LC market. (bussfuses.net)
Compounds2
- Following the positive ID of the five compounds of interest and their elution points, the drug mixture was then ready for a preparative LC-UV run on the puriFlash ® 5.250 iELSD. (advion.com)
- The purity of these compounds can be verified using analytical scale HPLC. (advion.com)
Prep6
- The highly pure cordycepin obtained by prep‑HPLC demonstrated inhibitory activity against LigA from various bacteria in vitro. (spandidos-publications.com)
- Prep-HPLC can be used to provide purified samples on a kilogram, or even ton, scale. (spandidos-publications.com)
- Also, prep-HPLC is an effective technology currently used for natural product isolation in industry ( 17 ). (spandidos-publications.com)
- The present study describes a new method of obtaining high-purity cordycepin by prep-HPLC, which may be useful for the large-scale isolation of cordycepin in the future. (spandidos-publications.com)
- Protect Your Investment - Hamilton guard columns protect analytical, semi-prep and preparative HPLC columns. (thomassci.com)
- Semi-prep/preparative holders are available only in stainless steel. (thomassci.com)
Chromatograms2
- Structure and HPLC chromatograms of cordycepin and the broad-spectrum adenosine-analog. (spandidos-publications.com)
- The semi-preparative HPLC method resulted in reproducible chromatograms for the seventeen fractionations of MWF. (cdc.gov)
Purity1
- Due to high loadability and further characteristics like excellent stability these HPLC columns packed with high purity NUCLEODUR C18 HTec silica gel are the choice for up-scaling to preparative scale. (mn-net.com)
Fraction1
- The intuitive preparative software PurityChrom controls the compact system and regulates the fraction collection via a 12-port fractionating valve. (gmi-inc.com)
Separation2
- The Separation Science Slam held by laboratory instrument manufacturer KNAUER with the support of The Analytical Scientist magazine on June 19 at the HPLC Conference 2023 in Düsseldorf, Germany, was a great success! (knauer.net)
- HPLC fractionation of cod head hydrolysate and sardine autolysate was necessary to evidence biological activity, whereas HPLC separation of shrimp hydrolysate exhibited low biological activity fractions. (nofima.no)
Methods1
- Unlike traditional preparative LC column packing methods, the Axia packing method offers increased sorbent bed density for increased performance and eliminates media bed collapse as a source of premature column failure in preparative HPLC/SFC columns. (phenomenex.com)
KNAUER3
- KNAUER has passed the certification audit according to EN ISO 13485. (knauer.net)
- In an increasingly competitive HPLC market where differentiation is challenging, KNAUER is committed to providing smart solutions that offer unique benefits to its customers. (knauer.net)
- They are available for all preparative KNAUER column dimensions and up to 100°C. Do you need to heat up columns with different dimensions or up to other temperatures? (knauer.net)
Detection1
- Humic substances are classified into three derived phenols generated by CuO oxidation are based on subcategories: humin, humic acid, and fulvic acid, based on GC-MS, HPLC with diode array detection (DAD) and CE- extractability at different pH values [3]. (lu.se)
Tasks1
- Regardless if you want to reduce viscosity or improve your performance, the column sleeves are a very compact and cost-effective solution for your preparative column heating tasks. (knauer.net)
Biological activity1
High1
- Due to high loadability and further characteristics like excellent stability these HPLC columns. (mn-net.com)
Hardware1
- Our column heating sleeves are the perfect solution for thermostatting your preparative column hardware. (knauer.net)
Semi1
- New developments in terms eryped 400 of preparative and semi-preparative HPLC will generate a mass spectrum. (medes.ru)
Materials1
- Polymer particles with sizes in the range 3 to 20 µm are extensively used for analytical purposes such as environmental analysis including air samplers, solid-phase extraction materials, and HPLC columns. (hitachi-hightech.com)
Guard2
- For protection of your main HPLC column from contamination, it is recommended to use guard. (mn-net.com)
- The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. (mn-net.com)
Standard1
- C) HPLC chromatogram of purified cordycepin sample compared with the standard. (spandidos-publications.com)
Single1
- Axia packed preparative columns involve a single axial compression step, unlike conventional packed preparative columns. (phenomenex.com)
Columns7
- HPLC columns can be damaged by sudden pump starts and stops or extreme gradient changes. (mandel.ca)
- Like every preparative phase from MACHEREY-NAGEL it is offered in VarioPrep columns with axially adjustable end fittings. (chromatographytoday.com)
- For protection of your main HPLC column from contamination, it is recommended to use guard columns. (mn-net.com)
- CFD simulation of preparative HPLC columns with consideration of nonlinear isotherms. (gdch.de)
- Guard columns can provide many benefits when used upstream from an analytical, preparative, or semi-preparative column. (hamiltoncompany.com)
- In addition to protective uses, guard columns can be used as sacrificial preparative components. (hamiltoncompany.com)
- Traditionally, choose a few analytical columns packed with preparative stationary phase material for method development. (labmate-online.com)
UHPLC2
- Phenomenex has launched mixed-mode selectivities and particle sizes for UHPLC, HPLC and preparative work in the Luna column range. (foodnavigator.com)
- Shimadzu has introduced a model of the i-Series integrated HPLC and UHPLC (Ultra High Performance Liquid Chromatograph) systems family. (foodnavigator.com)
Analytical and preparative2
- This innovative RP18 phase is qualified for analytical and preparative applications due to its high selectivity, the good base deactivation and the outstanding mechanical and chemical stability. (chromatographytoday.com)
- Further fractionation of extracts can be performed by analytical and preparative HPLC -- the scale of which depends on the application. (labx.com)
Chromatographic4
- The aforementioned comparison proven that HSCCC could display higher effectiveness than HPLC so long as enantioseparation element in the chromatographic program was high plenty of. (sciencepop.org)
- Alternatively generally HPLC requirements reagent of chromatographic quality while HSCCC requirements reagent of just analytical or chemical substance grade. (sciencepop.org)
- Assessment of preparative HSCCC and HPLC was evaluated concerning chromatographic guidelines efficiency solvent and chiral selector consumptions. (sciencepop.org)
- 5. Preparative Thin Layer Chromatographic Separation Followed by Identification of Antifungal Compound in Cassia laevigata by RP-HPLC and GC-MS. Panigrahi, G., R. Maheshwari, V. Kumar, J. Prakash, S. Kumar, and J. Prabakaran. (nih.gov)
Pump1
- The integrated degasser and AZURA® inline filter are completing this analytical AZURA® P 6.1L HPLC pump and turn it into a work horse in the lab. (knauer.net)
Autosampler1
- Thank you for your interest in the Agilent 1100 Series - G2260A HPLC Preparative Autosampler (SKU:18545) To speak with a representative, please fill out the contact form below or call us directly. (cambridgescientific.com)
Yields2
- The remarkably high loadability of the C18 HTec phase allows high yields of the target products in preparative HPLC, even under extreme method conditions. (chromatographytoday.com)
- Preparative HPLC yields higher separation efficiency than HSCCC generally. (sciencepop.org)
Liquid1
- The latter analyses were done in the laboratory of P. E. Hare, Geophysical Laboratory, Carnegie Institute of Washington, D.C., using high-performance liquid chroma- tography (HPLC), and fluorescence detection of o-phthalaldehyde amino acid derivatives. (nih.gov)
Pumps1
- KNAUER high pressure pumps are well-known for their compactness and reliability as HPLC pumps as well as for dosing applications. (knauer.net)
Solvent4
- The lyophilized sample was purified by preparative RP-HPLC on a C4 column using a gradient of 40 to 100% solvent B over 60 min. (medscape.com)
- Though total solvent and total quantity of chiral selector consumed by HSCCC had been greater than HPLC its device efficiency (mg min?1) device solvent usage (mL mg?1) and device chiral selector usage (mmol mg?1) were lower than HPLC. (sciencepop.org)
- For the fixed phase a typical fixed phase column is essential for HPLC parting while just solvent could possibly be used because the fixed stage for HSCCC. (sciencepop.org)
- Optimization of cellular stage for preparative HPLC as well as the biphasic solvent systems for AR-C155858 preparative HSCCC had been accomplished. (sciencepop.org)
Fractions1
- The fractions were analyzed by electrospray ionisation mass spectrometry (ESI-MS) and analytical RP-HPLC and, where appropriate, pure fractions were combined to give final compound. (medscape.com)
Temperature1
- High-speed centrifuges, preparative ultracentrifuges, a scintillation counter, a French press, an ultrasonicator for cell disruption, variable Temperature preparative shakers and incubators, -80 °C freezers, and an autoclave, and a 600 ft 2 cold room. (nih.gov)
Reagent1
- Samples were analyzed directly or were dialyzed prior to analysis twice against 6-liter volumes of phosphate buffered saline, pH 7.4, 0.05% NaN, (PBS) containing 0.1% sodium dodecyl sulfate (SDS} and then twice against 1-liter volumes of 0.01% SDS prepared with Baker HPLC reagent water (J. T. Baker Chemical Co., Phillipsburg, NJ) at 25°C over a 24-hr period. (nih.gov)
Scale1
- Figure 3 is Chromatogram of the extract from Coptis japonica powder using semi-preparative HPLC scaled up from the analytical scale method. (jascoinc.com)
Method2
Guard column1
- The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. (mn-net.com)
System1
Phase1
- Table 2 Assessment of HPLC and HSCCC in enantioseparation of ��-cyclopentylmandelic acidity 4 Conclusions Enantioseparations of ��-substitution mandelic acids had been looked into by preparative HPLC and HSCCC with Horsepower-��-Compact disc or SBE-��-Compact disc because the chiral cellular phase chemicals. (sciencepop.org)
Selector1
- No maximum quality was noticed for enantioseparation of ��-methylmandelic acidity by HSCCC with SBE-��-Compact disc like a chiral selector but preparative HPLC offered satisfactory maximum quality for ��-methylmandelic acidity using the same chiral selector because the cellular stage additive. (sciencepop.org)
Cellular2
- Sample loading limitations for preparative HPLC with Horsepower-��-Compact disc as chiral cellular stage additive was 10 mg with moderate quality although it could reach 250 mg of racemate injected in preparative HSCCC with higher quality. (sciencepop.org)
- methylmandelic acidity had been attained by preparative HPLC with Horsepower-��-Compact disc and SBE-��-Compact disc as chiral cellular stage additive respectively. (sciencepop.org)
Analysis1
- The molecular weight of the LPs was confirmed by ESI-MS, with each product obtained at greater than 99% purity (by analytical RP-HPLC AUC analysis). (medscape.com)
Spectral1
- The third peak, representing a new compound eluting later than DY in HPLC, was purified and subjected to mass spectral and nuclear magnetic resonance (NMR) analyses and identified as 3-bromotyrosine. (usda.gov)
Application1
- A series of tools to help you navigate to the best HPLC column choice for your application. (agilent.com)
Mixture1
- The main purposes for using HPLC are for identifying, quantifying, and purifying the individual components of the mixture. (selectscience.net)
Compact1
- For enantioseparation of ��-cyclopentylmandelic acidity with AR-C155858 Horsepower-��-Compact disc as chiral portable stage additive both strategies could give sufficient maximum resolutions but preparative HSCCC demonstrated a much higher preparative capability than preparative HPLC. (sciencepop.org)
Found1
- An evaluation of preparative HSCCC and HPLC found in enantioseparation of ��-cyclopentylmandelic acidity was summarized in Desk 2. (sciencepop.org)