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Alkaline PhosphatasePhosphoprotein PhosphatasesAcid PhosphataseProtein Tyrosine PhosphatasesProtein Phosphatase 2Protein Phosphatase 1Phosphoric Monoester HydrolasesGlucose-6-PhosphataseIsoenzymesHypophosphatasiaBone and BonesPhosphatesPhosphorylationOsteoblastsOsteocalcinMolecular Sequence DataKineticsCalcification, PhysiologicDual-Specificity PhosphatasesPlacentaProtein Tyrosine Phosphatase, Non-Receptor Type 11cdc25 PhosphatasesProtein Tyrosine Phosphatase, Non-Receptor Type 1Amino Acid SequenceOkadaic AcidOsteogenesisProtein Tyrosine Phosphatase, Non-Receptor Type 6LiverMyosin-Light-Chain PhosphataseTetramisoleHistocytochemistryEscherichia coliPhosphorylase PhosphataseProtein Tyrosine Phosphatases, Non-ReceptorElectrophoresis, Polyacrylamide GelHomoarginineMolecular WeightCell LineCells, Culturedgamma-GlutamyltransferaseEthers, CyclicReceptor-Like Protein Tyrosine Phosphatases, Class 2Cell MembraneHydrogen-Ion ConcentrationCalciumBase SequenceSubstrate SpecificityIntestinesCell DifferentiationEnzyme ActivationPhosphatidate PhosphataseLevamisoleNitrophenolsPhosphorusSignal TransductionDual Specificity Phosphatase 1CattleTime FactorsNucleotidasesRabbitsClinical Enzyme TestsMutationAspartate AminotransferasesElectrophoresis, Cellulose AcetateMagnesiumZincTartratesReceptor-Like Protein Tyrosine Phosphatases, Class 3Enzyme InhibitorsCloning, MolecularBone Morphogenetic Protein 2Receptor-Like Protein Tyrosine Phosphatases, Class 4VanadatesProtein BindingOsteomalaciaMitogen-Activated Protein Kinase PhosphatasesOsteitis DeformansOrganophosphorus CompoundsBone DiseasesParathyroid HormoneRecombinant Fusion ProteinsChromatography, GelRNA, MessengerRecombinant ProteinsMicroscopy, ElectronPhosphoric Diester HydrolasesEnzyme InductionHot TemperatureCore Binding Factor Alpha 1 SubunitBinding SitesOxazolesMicrovilli4-NitrophenylphosphataseBacterial ProteinsDual Specificity Phosphatase 6Protein Tyrosine Phosphatase, Non-Receptor Type 2CalcineurinMembrane ProteinsElectrophoresisBiological Markers
SerumAlbuminAntibodyConjugatesEnzymeAntibodiesIsoenzymesCommonlyCREATININEEDTAConcentrationsSkeletalInhibitorsReagentsTissuesHodgkinMembraneHepatitisProteinEnzymesBonesTestsLevels are highPhysicianResultsSpotted feverSpecificHighSide effectsReagentBlood testHealthCellBusiness DaysReportProductionMeasuresActivitySpecialSampleGroup
Serum10
Albumin1
Antibody4
  • Conjugation of enzymes to antibodies involves the formation of a stable, covalent linkage between an enzyme [e.g., horseradish peroxidase (HRPO), urease, or alkaline phosphatase] and an antigen-specific monoclonal or polyclonal antibody in which neither the antigen-combining site nor the active site of the enzyme is functionally altered. (omicsonline.org)
  • The enzyme most commonly used in the immunoreagent (the antibody enzyme conjugate) preparation is horseradish peroxides. (omicsonline.org)
  • The selection of an optimal conjugation time for preparing alkaline phosphatase?antibody conjugates varies for different antibodies, in particular when monoclonal antibodies are used. (omicsonline.org)
  • It is then incubated with a primary antibody followed by a detection antibody or a primary antibody conjugated to a detection molecule (commonly HRP or alkaline phosphatase). (wikipedia.org)
Conjugates2
Enzyme3
  • ALP" stands for "alkaline phosphatase," another name for this enzyme. (cura4u.com)
  • Alkaline Phosphatase (ALP) is an enzyme found in the bones, liver and bile ducts. (sarwarpro.com)
  • Polyphenols found in the enzyme preparation were found to interfere with the reduction of the dinitrosalicylic acid reagent. (who.int)
Antibodies2
Isoenzymes1
  • Although alkaline phosphatases are present in different body tissues and have different physiochemical properties, they are true isoenzymes because they catalyze the same reaction. (statpearls.com)
Commonly1
  • Alkaline Phosphatase (ALP) is commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry. (bbisolutions.com)
CREATININE1
EDTA2
  • ALP is stable for 48 hours at 4°C and 2 months at - 20°C. EDTA, oxalate, fluoride, citrate are known inhibitors of ALP and should be avoided in sample preparation. (gentaur.be)
  • EDTA interferes with alkaline phosphatase. (labpedia.net)
Concentrations2
Skeletal1
  • Appreciation of the significant amounts of F in some modern preparations of tea suggested to us that [skeletal fluorosis] caused our patient's high bone mass when she recounted her remarkable volume of instant tea consumed over three decades. (fluoridealert.org)
Inhibitors4
  • Protease and phosphatase inhibitors are essential components of most cell lysis and protein extraction procedures. (thermofisher.com)
  • In whole cells, protease and phosphatase activities are tightly regulated by compartmentalization or inhibitors to prevent indiscriminate damage to cellular proteins and to maintain proper function of signaling pathways. (thermofisher.com)
  • Protease and phosphatase inhibitors can be added to the lysis reagents in order to prevent degradation of extracted proteins, and to obtain the best possible protein yield and activity following cell lysis. (thermofisher.com)
  • Proteases inhibitors are nearly always needed, while phosphatase inhibitors are required only when phosphorylation states (activation states) are being investigated. (thermofisher.com)
Reagents1
  • This diagnostic method uses compatible reagents for all involved assays and standard fluidics for automatic sample preparation at POC. (indianabiosciences.org)
Tissues5
  • Like other calcium channel antagonists, diltiazem decreases sinoatrial and atrioventricular conduction in isolated tissues and has a negative inotropic effect in isolated preparations. (nih.gov)
  • Even though alkaline phosphatases are present in many tissues throughout the body, their precise physiological function remains largely unknown. (statpearls.com)
  • Biocare Medical has developed a Mouse-on-Rat alkaline phosphatase (AP) polymer detection system with minimal cross-reactivity to rat tissues. (micro-shop.pl)
  • It can be used with formalin-fixed paraffin-embedded tissues, floating sections, frozen sections and cell culture preparations. (micro-shop.pl)
  • dc.title: A Preliminary study of the invertase activity in coconut dc.contributor.author: ALLES, NH dc.description.abstract: AB : A ` particulate ` invertase preparation of low activity (sedimenting in the 900 g fraction) was extracted from the stalk and mesocarp tissues of coconut (Cocos nucifera L.). The activity o invertase was found to increase linearly with the time of incubation (0 - 3h). (who.int)
Hodgkin1
Membrane2
Hepatitis1
Protein2
  • Disruption of cellular and tissue architecture during protein extraction distorts the in vivo state by making all proteins potentially accessible for degradation or modification by endogenous proteases and phosphatases. (thermofisher.com)
  • They also reduce cholesterol, tri-glyceride, and alkaline phosphatase levels along with increased total protein contents 6 . (ijpsr.com)
Enzymes3
  • What are proteases (proteolytic enzymes) and phosphatases? (thermofisher.com)
  • Proteases and phosphatases are important enzymes in a variety of biochemical pathways in living cells. (thermofisher.com)
  • Thus, while numerous compounds have been identified and used to inactivate or block these enzymes, no single chemical is effective for all types of proteases and phosphatases (see tables below). (thermofisher.com)
Bones2
Tests4
Levels are high1
Physician1
Results1
  • The blood test is performed by drawing blood from the arm and sending the sample to the lab for analysis.You'll find that out from the results if your alkaline phosphatase levels are low, high, or within the normal range. (cura4u.com)
Spotted fever1
  • For serodiag- Shizuoka Prefecture, Japan, who had frequently predominates by A. phago- nosis of HGA, A. phagocytophilum rickettsiosis-like symptoms, were sus- cytophilum cultured in HL60 cells ( 6 ) propagated in HL60 cells is usually pected to have Japanese spotted fever as representatives for the preparation used as an antigen, especially by in- or scrub typhus, but they were sero- of recombinant proteins. (cdc.gov)
Specific5
  • Known phosphatases are specific for cleavage of either serine-threonine or tyrosine phosphate groups. (thermofisher.com)
  • While elevated alkaline phosphatase levels can sometimes be seen in kidney cancer, it is not the most common or specific marker for this condition. (cura4u.com)
  • Alkaline phosphatases are classified as tissue-specific and tissue-nonspecific types. (statpearls.com)
  • Alkaline phosphatases found in the intestine, placenta, and germinal tissue are tissue-specific. (statpearls.com)
  • We have also developed a series of preparations to provide a range of specific activities to suit a wide range of applications. (bbisolutions.com)
High4
Side effects1
  • Ease of availability, low cost, and least side effects make plant-based preparations the main key player of all available therapies, especially in rural areas 1, 2 . (ijpsr.com)
Reagent1
  • This evolving marketplace creates exciting opportunities for a variety of new instruments, reagent systems, and auxiliary products, such as specimen preparation devices, controls, and calibrators. (marketresearch.com)
Blood test2
Health2
Cell1
  • Cell lysis disturbs the carefully controlled cellular environment, allowing proteases and phosphatases to become unregulated. (thermofisher.com)
Business Days1
  • The delivery time for the electronic version of this report is between 1-3 business days to allow the preparation of an updated version prior to dispatch. (marketresearch.com)
Report1
Production1
Measures1
Activity1
  • BioAssay Systems' QuantiChromTM Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. (gentaur.be)
Special1
Sample2
Group1