AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and Processes
In Vitro Oocyte Maturation TechniquesOocytesOvarian FollicleOogenesisEmbryo Culture TechniquesOvulationGranulosa CellsTheca CellsFollicle Stimulating HormoneFollicular PhaseOvaryProgesteroneFollicular FluidHair FollicleChorionic GonadotropinLuteinizing HormoneEstradiolGonadotropins, EquineMeiosisCumulus CellsFollicular AtresiaCorpus LuteumGonadotropinsProestrusEstrusLuteinizationAndrostenedioneCattleGonadotropin-Releasing HormoneOvulation InductionRNA, MessengerGonadotropins, PituitaryInhibinsSuperovulationXenopus laevisFertilizationPregnancyEstrous CycleHorsesFertilization in VitroOocyte RetrievalOocyte DonationReceptors, LHAromataseSexual MaturationXenopusCells, CulturedChickensGrowth Differentiation Factor 9SheepMaturation-Promoting FactorOvumReceptors, GonadotropinBone Morphogenetic Protein 15Embryonic DevelopmentGenes, mosSteroidsSwine
OvariesOvarianOvulationFertilizationDevelopmentalEmbryoTransferActivityMetaphase II oocytesOxidative stressFollicular FluidMorphological qualityMorphologyZona pellucidaOogenesisOvaryCompetenceICSIEmbryosInfertilityApoptosisImmatureGranulosaDeclineSignificantlyAssessInfluencesDevelopmentQualityReproductiveProteinPrimaryMaturePatientsProduceCompleteImportantProductionGrowthHumanSelectionPotentialHigh
Ovaries1
  • Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)
Ovarian2
  • in 2015 showed that supplementation of CoQ10 in an aged animal model delayed decline in ovarian reserve, restored oocyte mitochondrial gene expression, and improved mitochondrial activity with a significant reduction in oocyte aneuploidy. (arvigen.com)
  • The number of ovarian follicles and corpora lutea also were not affected by treatment. (ejast.org)
Ovulation2
  • These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
  • Therefore, a potential conflict was observed between low-fat diets, which stimulate follicle growth and ovulation, and high-fat diets (insulin-depressing), which improve the development of the embryo and pregnancy outcome [ 15 ]. (ejast.org)
Fertilization1
  • The present review discusses methods to improve oocyte quality in older women and new stimulation protocols that may improve the number of mature oocytes retrieved during an in vitro fertilization cycle. (arvigen.com)
Developmental2
  • Increased IGFBP-4 and -5 protease along with lower amounts of IGFBP-4 binding activity and greater amounts of free IGF-I are some of the earliest developmental changes documented in bovine growing antral follicles. (bioone.org)
  • As a result, these aged mice had more oocytes with stimulation, the developmental potential of the oocytes was improved, and more offspring were born compared to old animals receiving placebo 1. (arvigen.com)
Embryo1
  • Moreover, the in vitro experiment demonstrated high glucose amounts' deleterious effect on embryo development [ 3 ]. (ejast.org)
Transfer1
  • In vivo development was assessed after transfer of injected oocytes to the oviducts of recipient mares. (bioone.org)
Activity1
  • In some species (e.g., bovine), evidence indicates that decreases in IGFBP-4 and -5 levels in estrogen-dominant preovulatory follicles are likely due, in part, to increased protease activity, whereas lower levels of IGFBP-2 are not due to increased proteolysis. (bioone.org)
Metaphase II oocytes2
  • A total of 1496 metaphase II oocytes ( n = 602 for phenotype A combining PCOM + HA + OA, n = 462 oocytes for phenotype C: PCOM + HA, and n = 432 for phenotype D: PCOM + OA) were assessed. (biomedcentral.com)
  • Secondary outcomes include the total number of retrieved metaphase II oocytes, average daily dose of gonadotropin, total gonadotropin dose and duration of ovarian stimulation, cycle cancellation rate, top quality embryos rate, blastocyst formation rate, embryo implantation rate, clinical pregnancy rate, early miscarriage rate and endometrial thickness on trigger day. (bvsalud.org)
Oxidative stress2
  • Oocyte-specific deletion of Gsα induces oxidative stress and deteriorates oocyte quality in mice [1] "The stimulatory heterotrimeric Gs protein alpha subunit (Gsα) is a ubiquitous guanine nucleotide-binding protein that regulates the intracellular cAMP signaling pathway and consequently participates in a wide range of biological events. (edu.au)
  • Numerous studies have shown that its mechanism may be related to a series of changes such as increased oocyte apoptosis, oxidative stress disorder, energy conversion disorder, and endocrine and metabolic abnormalities caused by age[2-6]. (researchsquare.com)
Follicular Fluid11
  • Follicular fluid and its contents representing the oocyte environment are now gaining more attention because not only of its crucial influence on oocyte developments but also due to easy isolation of follicular fluid with every case of ICSI. (scirp.org)
  • Objectives: The aim of this study was to detect the possible effect of follicular fluid lactoferrin on oocyte quality and hence pregnancy rate in ICSI cycles. (scirp.org)
  • Methods: Follicular fluid was obtained from 64 patients undergoing Intra-cytoplasmic sperm injection (ICSI) procedure in Ain Shams University Maternity Hospital. (scirp.org)
  • Follicular fluid (FF) was collected at the time of oocyte harvesting. (scirp.org)
  • Conclusion: Lactoferrin level in follicular fluid does not correlate with oocytes quality or pregnancy rate. (scirp.org)
  • Follicular fluid (FF), which consists of products of components that crossed the blood follicular barrier as well as products of the secretory activity of granulosa and theca cells, represents the oocyte microenvironment so it plays a vital role in oocyte development and maturation. (scirp.org)
  • Follicular fluid is an important external environment for the growth and development of oocytes. (researchsquare.com)
  • A thorough identification of specific components in follicular fluid can better the existing understand of intracellular signal transduction and reveal potential biomarkers of oocyte health in women undergoing assisted reproductive therapy. (researchsquare.com)
  • A combination of Ultra-high-performance liquid chromatography and high resolution mass spectrometry techniques were used to analyze the follicular fluid of 230 patients enrolled for the IVF cycle. (researchsquare.com)
  • The quality of oocytes decline with age,and the lipid composition in follicular fluid also changes,The lipid metabolism that changes with age may be related to the quality of oocytes.The main differences were in lipid metabolites. (researchsquare.com)
  • Follicular fluid is the culture medium in which an oocyte grows and differentiates. (researchsquare.com)
Morphological quality1
  • The present study is the first to show that the PCOS phenotype (notably the presence vs. absence of OA and/or HA) is not significantly associated with the morphological quality of oocytes. (biomedcentral.com)
Morphology3
  • The BCB-staining in high-quality oocytes did not have a significant impact on their maturation ability compared to a control group, but it might be useful for selecting developmentally competent oocytes with low morphology characteristics. (macvetrev.mk)
  • It is believed that one of the causes is an early stage of degeneration of oocytes with normal morphology ( 7 ). (macvetrev.mk)
  • Likewise, there were no significant intergroup differences in oocyte morphology. (biomedcentral.com)
Zona pellucida1
  • The released oocyte is surrounded by a thick specialised extracellular matrix, the zona pellucida , that in turn is covered in layers of cells, the granulosa layer . (edu.au)
Oogenesis3
  • 8 ) suggested a simple method for selection of porcine oocytes based on the enzymatic activity of glucose-6-phosphate dehydrogenase (G6PDH), a component of the pentose cycle synthesized during the oogenesis. (macvetrev.mk)
  • Complete in vitro oogenesis: retrospects and prospects [3] "In reality the vast majority of oocytes formed from primordial germ cells (PGCs) will undergo apoptosis, or other forms of cell death. (edu.au)
  • However, to recapitulate mammalian oogenesis and produce fertilizable oocytes in vitro is a complex process involving several different cell types, precise follicular cell-oocyte reciprocal interactions, a variety of nutrients and combinations of cytokines, and precise growth factors and hormones depending on the developmental stage. (edu.au)
Ovary2
  • The primary objective of the present study of women participating in an ICSI program was to determine whether the morphologic quality of oocytes was related to the polycystic ovary syndrome (PCOS) phenotype. (biomedcentral.com)
  • The oocyte (eggs, ova, ovum) is arrested at an early stage of the first {{meiosis))(first meiotic) division as a primary oocyte (primordial follicle) within the ovary . (edu.au)
Competence2
  • Oocyte quality and maturity are the most important determinants of blastocyst developmental competence ( 3 ). (macvetrev.mk)
  • Therefore, MTOR-dependent pathways in primordial or growing oocytes differentially affected downstream processes including follicular development, sex-specific identity of early granulosa cells, maintenance of oocyte genome integrity, oocyte gene expression, meiosis, and preimplantation developmental competence. (edu.au)
ICSI2
  • Assessment of oocyte quality to avoid overproduction of embryos is now considered an important goal in ICSI cycles. (scirp.org)
  • Although in classic Intra Cytoplasmic Sperm Injection (ICSI) cycles selection is mainly done for embryos and not for follicles. (scirp.org)
Embryos1
  • On the one hand, Alikani and colleagues reported reduced pregnancy and implantation rates in women with exclusive replacement of embryos, originating from dysmorphic oocytes regardless of the oocyte abnormality features [ 21 ]. (biomedcentral.com)
Infertility2
  • meiosis in Gsα-deficient oocytes precociously resumed in only 43% of antral follicles from mutant mice, indicating that alteration of meiotic pause was not the key factor in infertility. (edu.au)
  • Here we show that conditional knockout (cKO) of Mtor in either primordial or growing oocytes caused infertility but differentially affected oocyte quality, granulosa cell fate, and follicular development. (edu.au)
Apoptosis1
  • GV oocytes from mutant mice showed early-stage apoptosis. (edu.au)
Immature3
  • However, only 30-35% of immature oocytes in mammals reach the blastocyst stage ( 2 ). (macvetrev.mk)
  • The catalytic activity of G6PDH in immature oocytes on brilliant cresyl blue (BCB) renders it colorless ( 9 ). (macvetrev.mk)
  • Early oocytes are also classified as immature (germinal vesicle (GV) or metaphase I (MI) stage). (edu.au)
Granulosa1
  • Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice [2] "MTOR (mechanistic target of rapamycin) is a widely recognized integrator of signals and pathways key for cellular metabolism, proliferation, and differentiation. (edu.au)
Decline1
  • Meanwhile, the Gsα knockout-induced decline in oocyte quality and low developmental potential was partially rescued by antioxidant supplementation. (edu.au)
Significantly1
  • The phenotypes A, C and D did not differ significantly with regard to the proportion of normal oocytes (adjusted percentages (95%CI): 35.2% (31.5 to 39.1%), 25.8% (21.9 to 29.9%) and 34.0% (29.7 to 38.6%), respectively: adjusted p = 0.13). (biomedcentral.com)
Assess1
  • The aim of the current study was to assess the relationship between BCB staining in porcine oocytes with high and low morphological characteristics and its maturation rate. (macvetrev.mk)
Influences1
  • It directly or indirectly influences the fertility and up growth potency of oocytes thus indirectly affecting women's reproductive function.Changes in follicular microenvironment in older women may be responsible for altered protein function thus affecting the viability of oocytes. (researchsquare.com)
Development3
  • In an adult human female, the development of a primordial follicle containing an oocyte to a preovulatory follicle takes in excess of 120 days. (edu.au)
  • Among these metabolites, lipid metabolism undergoes significant changes that affect the development of oocytes thus causing reduced fertility in older women. (researchsquare.com)
  • These differential metabolites related to follicular development may provide possible detection and treatment targets for promoting oocyte health, and provide scientific basis for understanding the environment of oocyte development. (researchsquare.com)
Quality3
  • The selection of oocytes with high quality is not important only for in vitro production (IVP), but also for other procedures such as pronuclear microinjections, intracytoplasmic sperm injection and nuclear transplantation ( 4 ). (macvetrev.mk)
  • The primary criterion widely used for oocyte quality assessment is morphological classification. (macvetrev.mk)
  • Nevertheless, it is likely that the oocytes are of poor morphologic quality in cases of full-blown PCOS. (biomedcentral.com)
Reproductive1
  • In the reproductive system, despite Gsα being associated with oocyte meiotic arrest in vitro, the exact role of Gsα in female fertility in vivo remains largely unknown. (edu.au)
Protein1
  • Protein refolding, cleavage of fusion moieties and chromatography techniques are involved in the protein purification process. (cusabio.com)
Primary2
Mature1
  • A positive correlation between a number of mature oocytes and lactoferrin concentration was not found (r.101). (scirp.org)
Patients1
  • All patients in the post-gonadotoxic treatment group had their oocytes frozen. (bvsalud.org)
Produce1
  • A way to solve this limitation could be to produce large numbers of oocytes, in vitro, from stem cells. (edu.au)
Complete1
  • It has been established that G6PDH gets inactivated as soon as the oocytes complete their growth ( 4 ). (macvetrev.mk)
Important2
  • The assisted reproduction techniques in animal species remain an important and essential tool for safe and efficient breeding, as well as for the improvement of the genetic value of domestic animals ( 1 ). (macvetrev.mk)
  • It is highly important for these oocytes to be assessed prior to their use in IVP because they can be in different developmental stages ( 5 ). (macvetrev.mk)
Production2
  • Oocytes used for in vitro production are most frequently retrieved from ovaries obtained from slaughterhouses. (macvetrev.mk)
  • reported in vitro procedures that appear to reproduce efficiently these conditions allowing for the production, completely in a dish, of a relatively large number of oocytes that are fertilizable and capable of giving rise to viable offspring in the mouse. (edu.au)
Growth1
  • Oocytes with completed growth phase have blue colored ooplasm and are classified as BCB-positive (BCB+). (macvetrev.mk)
Human1
  • The breakdown of the germinal vesicle indicates a resumption of meiosis and the extrusion of the first polar body (1 PB) indicates completion of the first meiotic division in human oocytes. (edu.au)
Selection1
  • Brilliant cresyl blue (BCB) staining is a method used for selection of developmentally competent porcine oocytes, in addition to the standard morphological classification. (macvetrev.mk)
Potential1
  • Furthermore, the level of ROS (reactive oxygen species) and the mitochondrial aggregation increased, and antioxidant glutathione (GSH) content, ATP level, mtDNA copy number, and mitochondrial membrane potential decreased in Gsα-deficient oocytes. (edu.au)
High1
  • Due to the high enzymatic activity of G6PDH in growing oocytes, the BCB stain is metabolized, the oocytes remain unstained, and are categorized as BCB-negative (BCB-) ( 10 ). (macvetrev.mk)