Glycodelin-F reduces the blinding of spermatozoa to the zona pellucida which is mainly expressed in the ovary, and synthesised in the granulosa cells, has a function in principle similar to that of Glycodelin-A. It also binds the sperm head, thereby inhibiting acrosome reaction and sperm-egg binding. (wikipedia.org)
Polymerase chain re2
Peripheral karyotyping and polymerase chain reaction (PCR) techniques are highlighted. (drpankajtalwar.com)
Using polymerase chain reaction Mycoplasma hominis and Ureaplasma urealyticum were detected in cervical secretions of 19.2% and 13.7% of infertile women, and the presence of mycoplasma was significantly correlated with the presence of AZP-Ab and ASA. (who.int)
Spermatozoa3
Boar spermatozoa arising from the sperm-rich ejaculate fraction are reported to have a more stable plasma membrane and are more resistant to cold shock and premature acrosome reaction than spermatozoa from the whole ejaculate. (fapesp.br)
We also reveal using a combination of immunological techniques and proteomics analytical tools that the PDE10A isoform X4 is present in the area of the developing acrosome of spermatids and of the acrosome of mature spermatozoa. (plos.org)
However, this hypothesis began to be questioned when it was found that (i) acrosome-reacted sperm cells are still able to pass through extracellular layers and fertilize the oocyte (ii) most of the sperm cells undergo premature acrosomal reaction before they reach the oocyte. (nusl.cz)
As a major part of differentiation, sperm undergo extensive post-meiotic maturation en route to the oocyte. (silverchair.com)
Infertility2
The protamine status, chromatin structure, chromatin condensation, and acrosome reaction of sperm and assisted reproductive outcomes were determined in couples with different male infertility factors. (ecerm.org)
If it grows big enough, it may interfere with the implantation of embryo in the uterus leading to premature delivery, miscarriage and increasing the risk of infertility if they grow near the opening of both Fallopian tubes and distort the shape of uterine captivity. (worldwidehealth.com)
Important1
Therefore, we aimed to characterize proteomes of the acrosome and acrosome- reacted sperm cells, identify important proteins using advanced bioinformatics approaches and discuss the function of the acrosome as a vesicle that serves to store and process metabolites after the previous maturation process. (nusl.cz)
Women1
She is a patron of the Daisy Network a charity for women with premature ovarian failure. (uaesymposium.com)
Function1
Although it is evident that the acrosome is an essential structure, its main function has not been solved yet. (nusl.cz)
Normal2
En normal syklus kan være fra 21-36 dager, pluss/minus 4 dager hver måned, med eggløsning ca 14 dager før starten av menstruasjon i hvert enkelt tilfelle. (tjenester.org)
According to the study ejaculates from short (1-3 hours) compared with long (3-7 days) periods of abstinence showed increases in motile sperm count, sperm vitality, normal sperm morphology, acrosome reaction capacity, total antioxidant capacity, sperm mitochondrial membrane potential, high DNA stainability, and a decrease in the sperm DNA Fragmentation Index (DFI). (ivfclinicsworldwide.com)
Motility and acrosome reaction3
6. Enhancement of motility and acrosome reaction in human spermatozoa: differential activation by type-specific phosphodiesterase inhibitors. (nih.gov)
To address this deficit, we developed a fully-automated robotic screening platform that provided quantitative evaluation of compound activity against two key attributes of human sperm function: motility and acrosome reaction. (elifesciences.org)
Hence, a male contraceptive could work in a number of different ways - it could completely halt spermatogenesis, block an essential function of the spermatozoon (such a motility) or perturb a cellular process that results in a non-productive interaction with the oocyte (such as prematurely triggering the acrosome reaction, and/or by blocking exocytosis or inhibiting receptor binding). (elifesciences.org)
By this way it promotes the maintenance of membrane stability, thus avoiding the premature fusion of plasma membrane (PM) and outer acrosome membrane (OAM), which is mandatory for the exocytosis of acrosome content. (univaq.it)
Integrity6
Sperm acrosome integrity and induction of apoptosis were assessed using the acrosomal content marker PSA-FITC and annexin V kit, respectively. (medscape.com)
Sperm was collected from the epididymis and several parameters of sperm function, such as sperm density, motility, viability, mitochondrial function, acrosome integrity and morphology, were evaluated. (ua.pt)
Thus, the results obtained indicate that, together with impaired motility, the effect of lead toxicity on acrosome integrity, leading to premature reaction, may compromise the ability of sperm to fertilize the oocyte. (ua.pt)
The left caudal epididymis was used to count the sperm number and analyze motility, viability, morphological abnormalities, acrosome reaction, DNA integrity, and histone-protamine replacement in the spermatozoa. (ijfs.ir)
Mice exposed to sodium arsenite showed a significant decrease in the num- ber, motility, viability, normal sperm morphology and acrosome integrity of spermato- zoa compared to the control group. (ijfs.ir)
After thawing, we measured total and progressive mobility, vitality, acrosome integrity, markers of capacitation signaling pathway and nuclear quality. (biomedcentral.com)
Human sperm1
1. The cyclic GMP-specific phosphodiesterase inhibitor, sildenafil, stimulates human sperm motility and capacitation but not acrosome reaction. (nih.gov)
Sperm-egg1
Glycodelin-F reduces the blinding of spermatozoa to the zona pellucida which is mainly expressed in the ovary, and synthesised in the granulosa cells, has a function in principle similar to that of Glycodelin-A. It also binds the sperm head, thereby inhibiting acrosome reaction and sperm-egg binding. (wikipedia.org)
Membrane4
The spermatozoa are then able to undergo a unique exocytotic process called the acrosome reaction (AR) which facilitates penetration of the zona pellucida, the oocyte's outer glycoprotein shell and allows fusion with the oocyte's plasma membrane, delivering its DNA. (elifesciences.org)
The viable population of the obtained gametes was characterized by median values 53.2% (4.5-80.3%) of intact sperm membrane, 50.8 (26.0-76.6%) of intact acrosome, 0.4% (0-98.7%) of fragmented chromatin, 5.9% (0.0-88.8%) of cells with high mitochondrial potential and 42.1% (8.3-63.7%) without lipid peroxidation. (bvsalud.org)
The viable population of the frozen/thawed gametes was characterized by median values: 18.4% (2.4-57.9%) of intact sperm membrane, 35.1 (11.9-56.7%) of intact acrosome, 0.07% (0-89.2%) of fragmented chromatin, 12.8% (0.0-49.7%) of cells with high mitochondrial potential and 16.3% (2.2-53.6%) without lipid peroxidation. (bvsalud.org)
Receptor1
Additionally, ZP3 serves as a primary sperm receptor and acrosome reaction-inducer, and ZP2 serves as a secondary sperm receptor during fertilization. (karger.com)
Intact1
However, a decrease in the percentage of intact acrosomes was also observed, mirroring a lead-induced premature acrosome reaction. (ua.pt)