• Inorganic pyrophosphatase (or inorganic diphosphatase, PPase) is an enzyme (EC 3.6.1.1) that catalyzes the conversion of one ion of pyrophosphate to two phosphate ions. (wikipedia.org)
  • The form of inorganic pyrophosphatase isolated from Thermococcus litoralis was found to contain a total of 174 amino acid residues and have a hexameric oligomeric organization (Image 1). (wikipedia.org)
  • Though the precise mechanism of catalysis via inorganic pyrophosphatase in most organisms remains uncertain, site-directed mutagenesis studies in Escherichia coli have allowed for analysis of the enzyme active site and identification of key amino acids. (wikipedia.org)
  • In particular, the binding of pyrophosphate (PPi) to the effector site of inorganic pyrophosphatase increases its rate of hydrolysis at the active site. (wikipedia.org)
  • Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. (wikipedia.org)
  • By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for beta oxidation. (wikipedia.org)
  • Each of these two steps is reversible under biological conditions, save for the additional hydrolysis of PPi by inorganic pyrophosphatase. (wikipedia.org)
  • Examination of prokaryotic and eukaryotic forms of soluble inorganic pyrophosphatase (sPPase, Pfam PF00719) has shown that they differ significantly in both amino acid sequence, number of residues, and oligomeric organization. (wikipedia.org)
  • INORGANIC PYROPHOSPHATASE (E.C.3.6.1.1) catalyzes the hydrolysis of phosphoester bonds of inorganic pyrophosphate [P 2 O 7 4- ], thereby releasing two orthophosphate molecules. (bioassaysys.com)
  • Here, the crystal structure of a family I inorganic pyrophosphatase from Legionella pneumophila is reported at 2.0â Å resolution. (bvsalud.org)
  • In this study, a rapid visual strategy based on loop-mediated isothermal amplification (LAMP) with the addition of thermal inorganic pyrophosphatase and linked with an ammonium molybdate chromogenic buffer was established to detect Salmonella. (bvsalud.org)
  • LAMP was performed at 65°C for 45 min in the presence of thermal inorganic pyrophosphatase at a final concentration of 4 U ml-1, and 20 µl of the LAMP product was reacted with 50 µl of phosphate chromogenic buffer at 25°C for 15 min. (bvsalud.org)
  • Here, we demonstrate that the swelling at the tips of incompatible pollen tubes in pear (Pyrus bretschneideri [Pbr]) is mediated by the SI-induced acetylation of the soluble inorganic pyrophosphatase (PPA) PbrPPA5. (bvsalud.org)
  • L. pneumophila PPase (LpPPase) adopts a homohexameric assembly and shares the oligonucleotide/oligosaccharide-binding (OB) ß-barrel core fold common to many other bacterial family I PPases. (bvsalud.org)
  • sPPases are ubiquitous proteins that hydrolyse pyrophosphate to release heat, whereas H+-PPases, so far unidentified in animal and fungal cells, couple the energy of PPi hydrolysis to proton movement across biological membranes. (wikipedia.org)
  • Two types of inorganic diphosphatase, very different in terms of both amino acid sequence and structure, have been characterised to date: soluble and transmembrane proton-pumping pyrophosphatases (sPPases and H(+)-PPases, respectively). (wikipedia.org)
  • Family I PPases are essential enzymes found in all kingdoms of life and are responsible for maintaining the correct pyrophosphate equilibrium necessary to carry out nucleic acid and protein synthesis, and facilitate fatty acid beta oxidation. (bioassaysys.com)
  • By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for beta oxidation. (wikipedia.org)
  • PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. (nih.gov)
  • Thermostable soluble pyrophosphatase had been isolated from the extremophile Thermococcus litoralis. (wikipedia.org)
  • Humans possess two genes encoding pyrophosphatase, PPA1 and PPA2. (wikipedia.org)