BiomarkersCarcinogenicCompoundsAminesChromosomal2020ChemicalsMechanisticMutagenicApproachesMutationsTherapiesSubstancesAssaysVivoDetectionAmesBiomarkerPathwayPotentialSensitivityProteinBiologicalBEACONLiverMethodsHumansFindingsResponseLidocaineEvaluateDamageAnalyticalEffectsDoseGeneExpansionStudySingleProvideGuideResultsFoodMethodChemical
Biomarkers4
- The future of tipifarnib lies in achieving a better understanding of the mechanism of clinical response and the use of predictive biomarkers for patient selection. (hsp90-inhibitors.com)
- Multi-omics approaches have been applied to numerous clinical studies for better identification of clinical subtypes or drug resistance, prediction of effective combination therapies, and identification of predictive biomarkers to increase the response rate to targeted treatments. (molcells.org)
- Background Molecular subtyping of urothelial cancer (UC) has significantly advanced the understanding of bladder tumor heterogeneity and development of prognostic and predictive biomarkers. (bmj.com)
- A comprehensive understanding of DDR gene inactivation associated expression of immune regulatory genes could thus aid in expansion of current immunotherapies and predictive biomarkers for the design of patient-tailored combination treatments. (bmj.com)
Carcinogenic6
- The SOS chromotest is comparable in accuracy and sensitivity to established methods such as the Ames test and is a useful tool to screen genotoxic compounds, which could prove carcinogenic in humans, in order to single out chemicals for further in-depth analysis. (wikipedia.org)
- "Only with a zero tolerance of MOAH in food can the EU ensure the absence of this potentially carcinogenic contaminant," said Suzy Sumner, Head of Brussels Office, foodwatch international. (foodnavigator.com)
- The lowest dose tested (450 mg/m in mice, 900 mg/m in rats, 60 times SDA) was carcinogenic in both species. (drugcentral.org)
- Heterocyclic aromatic amines (HAA) are environmental and food contaminants that are potentially carcinogenic for humans. (peerj.com)
- The ToxTracker assay is a panel of six validated GFP-based mouse embryonic stem (mES) reporter cell lines that can be used to identify the biological reactivity and potential carcinogenic properties of compounds in a single test. (europa.eu)
- Stem cells are genetically stable and proficient in all cellular pathways required for accurate detection of potentially carcinogenic properties of compounds. (europa.eu)
Compounds6
- The SOS chromotest is a biological assay to assess the genotoxic potential of chemical compounds. (wikipedia.org)
- This test was developed as a practical complement or alternative to the traditional Ames test assay for genotoxicity, which involves growing bacteria on agar plates and comparing natural mutation rates to mutation rates of bacteria exposed to potentially mutagenic compounds or samples. (wikipedia.org)
- The SOS response plays a central role in the response of E. coli to genotoxic compounds because it responds to a wide array of chemical agents. (wikipedia.org)
- Firstly, because of its simplicity and rapidity, the SOS chromotest may be used as a screening test for a large number of potentially genotoxic compounds. (wikipedia.org)
- The updated US FDA report included a comprehensive review of biocompatibility and toxicology test reports, a critical review and risk assessment of tantalum nanoparticle toxicity, and ISO 10993-compliant toxicological risk assessments of extractable and leachable compounds. (gradientcorp.com)
- DNA adduct formation pathway through the prediction of potential genotoxic compounds) and detoxification of MeIQx in order to predict the behaviour of this environmental contaminant in the human liver. (peerj.com)
Amines1
- 2022. "A Rubric for Identifying Potentially Genotoxic Polycyclic Amines and Derivation of a Chemical Class-Specific TTC. (gradientcorp.com)
Chromosomal3
- There are a variety of well-established genotoxicity tests that can evaluate acquired DNA damage and chromosomal instability. (westminster.ac.uk)
- Mutagenesis: The mutagenic potential of lidocaine HCl has been tested in bacterial reverse (Ames) assay test in Salmonella, an in vitro chromosomal aberration assay using human lymphocytes and in an in vivo micronucleus test in mice. (drugcentral.org)
- Unlike nuclease editors, the BEAM-101 base editor is designed to avoid double stranded breaks during editing, which can result in unwanted chromosomal abnormalities and genotoxic stress. (beamtx.com)
20201
- 2020. "Predictive Toxicology Approaches for Medical Device Biocompatibility Assessment. (gradientcorp.com)
Chemicals6
- Secondly, it may allow the detection of genotoxic chemicals which yield false negatives in the Ames test (such as estradiol, a compound of growing concern). (wikipedia.org)
- Because no battery of tests is required on dietary supplement ingredients, results from safety tests common to other chemicals are not widespread, although they are available for some ingredients. (nationalacademies.org)
- ACuteTox set the very ambitious overall objective of developing an in vitro test strategy sufficiently robust and powerful to completely replace in vivo testing of acute toxicity of chemicals. (enhesa.com)
- Over 97% of all tested chemicals were classified correctly. (europa.eu)
- 2012). The ToxTracker assay: novel GFP reporter systems that provide mechanistic insight into the genotoxic properties of chemicals. (europa.eu)
- Recommended lists of genotoxic and non-genotoxic chemicals for assessment of the performance of new or improved genotoxicity tests: a follow-up to an ECVAM workshop. (europa.eu)
Mechanistic5
- Martin's research is focused on the nanoparticle-(mammalian)cell interaction, with a view to determining the mechanistic toxicological, immunological and genotoxic effects that nanoparticles, with varying physico-chemical characteristics, may cause at the cellular level by using advanced, next-level in vitro systems combined with state-of-the-art microscopy and bioanalytical approaches. (swansea.ac.uk)
- Another clear ambition of Martin's research is to create, study and promote alternative in vitro models that mimic in vivo environments, in order to gain a mechanistic understanding of how nanoparticles may, potentially, adversely impact human health. (swansea.ac.uk)
- Furthermore, by creating sophisticated in vitro multi-cellular models of different human organs in combination with state-of-the art microscopic and biochemical analyses, Martin is able to obtain a mechanistic understanding of the health risks posed by nanomaterials under realistic conditions, as well as gain valuable insight into the potential of in vitro as alternative test systems to in vivo methodologies. (swansea.ac.uk)
- The committee aims to catalyze adoption of new translational and predictive tools that guide decision-making based on mechanistic understanding of toxicological response. (hesiglobal.org)
- The marker has the potential to improve upon the low specificity of in vitro chromosome damage assays used in current testing and to aid drug development by providing mechanistic insights into transcriptional changes occurring in genes involved in key DNA damage pathways. (hesiglobal.org)
Mutagenic1
- One such marker is 8- OH-2-deoxy Guanosine (8-OHdG), a DNA lesion that is potentially mutagenic. (westminster.ac.uk)
Approaches1
- Validation of this predictive marker is critical for future therapeutic approaches and may generate important information that will restore interest in tipifarnib as an active agent in AML and MDS Annotated bibliography Agrawal AG, Somani RR. (hsp90-inhibitors.com)
Mutations3
- BEAM-101 aims to potentially alleviate the effects of mutations causing SCD by leading to increases in fetal hemoglobin (HbF), which inhibits hemoglobin S (HbS) polymerization. (beamtx.com)
- Fine-tuned responses at various degrees and scales within the human body are central to the homeostatic mechanism that copes with potentially harmful environmental perturbations, including pathogens, smoking, and drugs, and interacts with the genetic background arising from spontaneous somatic mutations and numerous germline variants. (molcells.org)
- In alignment with these concepts, recent immune checkpoint blockade (ICB) therapies in UC have demonstrated the predictive potential of mutations in the DNA damage repair (DDR) genes. (bmj.com)
Therapies1
- Discovery of non-genotoxic mechanisms through which p53 protein may be activated within tumor cells harboring wild-type p53 remains an important therapeutic objective, both in effort to alleviate traditional off-target chemotherapeutic side-effects and to prevent the unpredictable mutagenicity characteristic of these therapies. (biomedcentral.com)
Substances3
- Experts also looked at two different types of MOAH, concluding for one that it may contain genotoxic substances that can damage DNA in cells and may cause cancer. (foodnavigator.com)
- The data collection identified 2,255 tests that were deemed to be sufficiently reliable for the analysis, including reports for 232 substances with at least two test results. (nih.gov)
- CDC Laboratory rapidly developed assays to analyze potentially harmful and addictive substances in bronchoalveolar lavage (BAL) fluid collected from EVALI case patients. (cdc.gov)
Assays1
- 2-Nitrotoluene was genotoxic in a range of in vitro and in vivo assays, was notably clastogenic in human peripheral lymphocytes and formed DNA adducts in exposed rodents. (gc.ca)
Vivo2
- Instead, we will take the opportunity to streamline the BEACON trial while focusing the next phase of our investment in sickle cell disease on our Wave 2 programs for non-genotoxic conditioning, as well as our Wave 3 strategy for in vivo lipid nanoparticle delivery of base editors, continuing to leverage both HbF and Makassar editing strategies. (beamtx.com)
- BEAM-302 is a first-in-kind program targeting direct in vivo DNA correction of the disease-causing E342K mutation in patients with severe AATD, which could address both the lung and liver manifestations of the disease with a potentially one-time treatment. (beamtx.com)
Detection1
- The detection of sensitivity by skin testing is of doubtful value. (drugcentral.org)
Ames1
- It serves as a useful and cost effective complement to the traditional Ames test for a number of reasons. (wikipedia.org)
Biomarker3
- The predictive power of the ratio of RASGRP Hedgehog Pathway APTX gene expression not only provides a potentially important biomarker of response, it also suggests that responsive patients have underlying pathology related to abnormalities in the DNA excision repair pathway. (hsp90-inhibitors.com)
- As a result, numerous authors have suggested an introduction of sperm DNA damage testing into the andrological laboratory workup as an independent tool as DNA fragmentation apart from normal sperm morphology appears to be a reliable and more robust parameter than conventional semen analysis due to its low biological variability and thus is a useful biomarker that should be implemented in any andrological diagnostic workup [ 19 - 21 ]. (hindawi.com)
- Perform biomarker analyses to identify potential markers predictive of response patterns or side effect profiles 3. (rwjbh.org)
Pathway1
- As discussed later in this chapter, some studies have identified cytotoxicity of e-cigarette aerosols, potentially contributing to tissue repair and mitogenic response, which is another important pathway in the development of chemically induced cancers. (nationalacademies.org)
Potential2
- Scientists with CPSC , FDA , and NIEHS and collaborators compiled such a data set for human skin sensitization potential by collecting data from the scientific literature for human predictive patch tests that used the human maximization or human repeated insult patch test protocols. (nih.gov)
- We will collaborate with stakeholders in enhancing MSS through qualitative interviews and usability testing of potential enhancements. (rwjbh.org)
Sensitivity2
- The quantitative relationship between the in vitro susceptibility of human cytomegalovirus (CMV) or herpes simplex virus 1 and 2 (HSV-1 and HSV-2) to FOSCAVIR and clinical response to therapy has not been established and virus sensitivity testing has not been standardized. (nih.gov)
- Sensitivity test results, expressed as the concentration of drug required to inhibit by 50% the growth of virus in cell culture (IC 50 ), vary greatly depending on the assay method used, cell type employed and the laboratory performing the test. (nih.gov)
Protein2
- Two genes play a key role in the SOS response: lexA encodes a repressor for all the genes in the system, and recA encodes a protein able to cleave the LexA repressor upon activation by an SOS inducing signal (caused in this case by the presence of a genotoxic compound). (wikipedia.org)
- Since the chemical tested may inhibit protein synthesis at higher concentrations, which would lead to an underestimation of B-galactosidase induction, alkaline phosphatase is assayed simultaneously with β-galactosidase in order to scale the data to survivability of the cells. (wikipedia.org)
Biological1
- Such type of study can provide novel frameworks to untangle biological phenomena or models to test certain hypotheses using various datasets. (molcells.org)
BEACON1
- We are also accelerating our overall BEAM-101 development program, where we see an opportunity to potentially seek regulatory approval on data generated from the BEACON trial. (beamtx.com)
Liver1
- This newly formed group is planning a proof of concept study to see if Cell Painting data (with or without transcriptomic data) can be predictive of existing rat liver data. (hesiglobal.org)
Methods1
- Developed and maintained by the American Herbal Products Association (AHPA), the AHPA Botanical Identity References Compendium is a centralized source of information on physical characteristics and test methods that can be used to determine the identity of plant species and articles of trade obtained from these plants. (enhesa.com)
Humans1
- Because humans are the primary subject of interest for regulatory safety testing, it is advantageous to have human reference data available for evaluation of NAMs for assessing chemical safety. (nih.gov)
Findings2
- Findings revealed one in eight products tested was contaminated. (foodnavigator.com)
- Conclusion Findings from our study provide compelling evidence for co-expression of multiple immune checkpoint genes including, PD-1 , PD-L1, IDO1, TIGIT, TIM-3, TGFB1, LAG3, and others, that potentially contribute to compensatory immune evasion in bladder tumors. (bmj.com)
Response2
- If using a micro-plate reader, the test is quantitative and dose-response curves have an initial linear region. (wikipedia.org)
- Groves FD, Zhang L, Chang YS, Ross PF, Casper H, Norred WP, You buy, Fraumeni JF Jr. Fusarium rates in response and growth drugs in a MCF-7 contamination for predictive surface in Shandong Province, China. (xn--drpverein-rahe-vpb.de)
Lidocaine1
- Lidocaine and prilocaine cream contains no preservative, however it passes the USP antimicrobial effectiveness test due to the pH. (themeditary.com)
Evaluate1
- Wilcoxons rank sum test was used to evaluate statistically signifi cant changes. (ampk-signal.com)
Damage1
- These types of tests can be utilized to inform susceptibility to disorders that are strongly associated with genome damage, such as cancer. (westminster.ac.uk)
Analytical1
- Foodwatch tests have proven again and again that it is possible for the food industry to have no MOAH detectable in food, when tested to the highest analytical standards of the JRC. (foodnavigator.com)
Effects1
- We will conduct a RCT comparing MSS and a non-interactive educational webpage with 300 survivors to test its effects on SSE. (rwjbh.org)
Dose1
- None of the protocol variables such as test type, skin patch size, sample size, or dose applied were associated with high or low variability. (nih.gov)
Gene1
- The test is a colorimetric assay which measures the expression of genes induced by genotoxic agents in Escherichia coli, by means of a fusion with the structural gene for β-galactosidase. (wikipedia.org)
Expansion1
- This newly formed group is exploring the use of error corrected sequencing to detect clonal expansion of non-genotoxic carcinogens. (hesiglobal.org)
Study1
- Not applicable - Since this is an in vitro study there is no information on test animals. (europa.eu)
Single2
- The effect of variation in vehicle used could not be analyzed because the majority of tests used a single vehicle. (nih.gov)
- We showed that LGO was more genotoxic after repeated exposure than single exposure due to persistent lung inflammation. (biomedcentral.com)
Provide1
- NLM developed ALTBIB to provide access to PubMed citations for users seeking information on alternatives to animal testing. (nih.gov)
Guide1
- Treatment history and, when available, genotypic or phenotypic testing should guide the use of Darunavir/ritonavir. (wikidoc.org)
Results1
- HACCP storage has an shared favorite of an vast method end in the wealth of results, and these should know therapists for outbreak, maize, and test from milk. (xn--drpverein-rahe-vpb.de)
Food1
- The legal obligation to use the highest standards of testing possible and to deliver food free from detectable MOAH has been overdue for many years. (foodnavigator.com)
Method1
- Antifungal activity of EO and fractions were tested by a broth microdilution method, whereby minimum inhibitory concentration (MIC) was determined against several fungal organisms (Candida albicans, Candida krusei, Candida parapsilosis, Candida tropicalis, Cryptococcus gattii, and Cryptococcus neoformans). (researchgate.net)
Chemical1
- The SOS chromotest consists of incubating the E. coli with increasing concentrations of the chemical to be tested. (wikipedia.org)