To study the cellular mechanisms for this post-transcriptional control and to determine the contribution of mRNA stability to GAP-43 gene expression, we examined the effects of several agents that affect PC12 cell differentiation on the level of induction and rate of degradation of the GAP-43 mRNA. (rupress.org)
The aim of this course is to provide an overview of post-transcriptional gene regulations at multiple levels, including (pre-)messenger RNA splicing, polyadenylation, modifications (epitranscriptome), localization, translation and degradation. (eu-life.eu)
Post-transcriptional control of animal histone gene expression: not so different after all. (elsevierpure.com)
Overall, our data suggest that butyrate can reduce the expression of inflammatory genes not only by transcriptional regulation, but also by post-transcriptional regulation via inhibition of mRNA stabilizing proteins. (metu.edu.tr)
Post-transcriptional regulation, via 5′-UTRs (5′-untranslated regions), plays an important role in the control of eukaryotic gene expression. (silverchair.com)
Efficient use of artificial micro-RNA to downregulate the expression of genes at the post-transcriptional level in Arabidopsis thaliana. (bvsalud.org)
Role of Post-transcriptional Gene Regulation and. (hhmi.org)
From 2017, she joined King's College London as a Lecturer and principal investigator of the Epigenetics and Post-transcriptional Gene Regulation group. (kcl.ac.uk)
Besides classical gene regulation, miRNAs have emerged as post-transcriptional regulators of angiogenesis. (plos.org)
Post-transcriptional modifications including alternative splicing (AS) events, fusion genes, and nonsense-mediated mRNA decay (NMD) events were found. (usda.gov)
Virus-induced gene silencing (VIGS) can be harnessed to sequence-specifically degrade host transcripts and induce heritable epigenetic modifications referred to as virus-induced posttranscriptional gene silencing (ViPTGS) and virus-induced transcriptional gene silencing (ViTGS), respectively. (ed.ac.uk)
While differential gene expression is among the most important of these processes, relatively little is known about other sources of transcriptional variation. (lu.se)
Previous work suggests that alternative splicing plays an extensive and functionally unique role in transcriptional plasticity, though plastically spliced genes may be more constrained than the remainder of expressed genes. (lu.se)
Genes with unique transcriptional changes through diapause consistently had the lowest nucleotide diversity, and this effect was consistently stronger among genes that were differentially spliced compared to those with just differential expression through diapause. (lu.se)
Although the plant and animal kingdoms were separated more than 1,6 billion years ago, multicellular development is for both guided by similar transcriptional, epigenetic and posttranscriptional machinery. (lu.se)
MicroRNAs (miRNAs) are an integral part of the post-transcriptional machinery of gene expression and have been implicated in the carcinogenic cascade. (cdc.gov)
Expression31
Posttranscriptional regulation of GAP-43 gene expression in PC12 cells through protein kinase C-dependent stabilization of the mRNA. (rupress.org)
Therefore, as the name indicates, it occurs between the transcription phase and the translation phase of gene expression. (wikipedia.org)
In short, the dsRNA sequences, which will be broken down into siRNA inside of the organism, will match up with the RNA to inhibit the gene expression in the cell. (wikipedia.org)
As an interesting fact, there are some mechanisms that attack this nuclear export process to regulate gene expression. (wikipedia.org)
The expression of histone genes is cell cycle controlled and coupled to DNA replication, to ensure the packaging of replicated DNA into chromatin. (elsevierpure.com)
Butyrate is a SCFA that was reported to alter the mRNA stability of inflammatory genes by increasiing the expression of the RNA binding protein (RBP) Tristetraprolin (TTP). (metu.edu.tr)
We have hypothesized that butyrate may regulate gene expression post-transcriptionally through global effects on the expression or cytoplasmic trans location of RBPs. (metu.edu.tr)
Recent analyses of the mammalian transcriptome suggest that most of the genes express multiple alternative 5′-UTRs and inappropriate expression of these regions has been shown to contribute to the development of carcinogenesis. (silverchair.com)
Micro-RNAs are cellular components regulating gene expression at the post-transcription level. (bvsalud.org)
The expression of the two target genes was checked through quantitative real-time polymerase chain reaction to confirm reduced transcript levels for AtExpA8 and AHL25. (bvsalud.org)
This technology could be used to prevent the expression of exogenous and invading genes posing a threat to the normal cellular physiology of the host plant . (bvsalud.org)
MicroRNAs (miRNAs) are potent effectors in gene regulatory networks where aberrant miRNA expression can contribute to human diseases such as cancer. (hindawi.com)
For a better understanding of the regulatory role of miRNAs in coordinating gene expression, we here present a systems biology approach combining data-driven modeling and model-driven experiments. (hindawi.com)
We are interested in gene-environment interactions, with a particular focus on the molecular mechanisms linking extracellular signalling to changes in gene expression and cell behaviour. (kcl.ac.uk)
Here the authors identify chromatin-bound RNAs and their binding sites in human embryonic stem cells suggesting that most chromatin-associated RNAs act proximal to their encoding loci and single RNAs are unlikely to alter gene expression. (nature.com)
miRNAs comprise a large group of endogenous non-coding RNAs that can block mRNA translation or negatively regulate mRNA stability and thereby play a central role in regulating gene expression [10] , [11] . (plos.org)
Daniel Kim's lab studies the role of long noncoding RNA molecules (lncRNAs) in gene expression and cancer. (ucsc.edu)
This study described gene expression in three alfalfa germplasms with different levels of tolerance to drought and high salt. (usda.gov)
The analyses of gene ontology and weighted gene co-expression network allowed us to identify key genes that play important roles in drought and salt stress tolerance in alfalfa. (usda.gov)
(B) Expressions of RNASET2 in human tissues were analyzed in the Human Protein Atlas database, and the resulting transcript expression values, denoted normalized expression (NX), were calculated for each gene in every sample. (frontiersin.org)
Mechanisms of regulation of gene expression by structured RNA molecules and RNA-protein complexes. (nyu.edu)
Both ViPTGS and ViTGS enable manipulation of endogenous gene expression without the need for transgenesis. (ed.ac.uk)
In this study, we explore the relationship between expression and splicing plasticity, along with the genetic diversity in those genes, in an ecologically consequential polyphenism: facultative diapause. (lu.se)
Splicing differs strongly between diapausing and direct developing trajectories but alters a smaller and functionally unique set of genes compared to differential expression. (lu.se)
Although more and animals and plants and regulate gene/protein expression more progressions have been made about the through direct complementarity between their 5' region pathogenesis of asthma in recent years, the increasing and the 3' untranslated region of target mRNAs. (cdc.gov)
The ability to regulate transgene expression will be crucial for development of gene therapy to the brain. (lu.se)
Using toxin-induced lesions we investigated to what extent transgene expression could be regulated in accordance with the activation of the endogenous GFAP gene. (lu.se)
An aspect of gene delivery to the brain that is currently being regulate the endogenous GFAP expression in a lesioned brain. (lu.se)
MicroRNAs (miRNAs) are small non-coding RNAs responsible for posttranscriptional regulation of gene expression. (lu.se)
MiRNAs5
Although microRNAs (miRNAs) are physically small, they have been shown to play an important role in gene regulation [ 1 ]. (hindawi.com)
The systems biology approach, combining data-driven modeling and model-driven experiments, provides a systematic and comprehensive perspective on the regulatory roles of miRNAs in gene regulatory networks [ 3 - 5 ]. (hindawi.com)
The application of the systems biology approach to the analysis of a gene regulatory network is demonstrated with a case study of the regulation of p21 by multiple miRNAs [ 4 ]. (hindawi.com)
SNPs in 6 miRNA processing genes and in 78 miRNAs which target genes known to be important in breast cancer among 906 African American (AA) and 653 European American (EA) cases and Author Manuscript controls enrolled in the Women's Circle of Health Study (WCHS). (cdc.gov)
We found a number of SNPs in miRNAs and processing genes in association with breast cancer overall or stratified by estrogen receptor (ER) status. (cdc.gov)
Translational3
This binding prospects to gene silencing by miRNA mediated degradation and translational suppression by disruption of the ribosomal complex.9 12 13 Therefore miRNA activity may regulate sets of genes for specific biological processes during development metabolism and homeostasis. (immune-source.com)
I pinpoint the most interesting and most likely MAP4K3-modulated genes at the translational level, and propose new paths worth validating and pursuing. (le.ac.uk)
RNA-protein multiome approach helps to discover that the posttranscriptional regulation of the translational machinery is crucial for the fidelity of cortical development. (elifesciences.org)
RNAs3
In the present study, artificial micro-RNAs were used to decrease the transcript level of two genes , AtExpA8 (encoding an expansin) and AHL25 (encoding an AT-hook motif nuclear localized protein ) in Arabidopsis thaliana . (bvsalud.org)
The backbone of the Arabidopsis endogenous MIR319a micro-RNA was used in a site-directed mutagenesis approach for the generation of artificial micro-RNAs targeting two genes . (bvsalud.org)
a map is constructed that shows interactions among molecular entities (such as genes, proteins and RNAs), using information from literature and databases. (hindawi.com)
Proteins4
His fields of research comprise RNA-mediated gene silencing processes with a focus on epigenetic phenomena, including studies on RNA-directed DNA methylation, the characterization of virus silencing suppressor proteins, the development of plant bioreactor platforms and viroid research. (degruyter.com)
The protein produced from this gene, called heterogenous nuclear ribonucleoprotein K (hnRNP K), attaches (binds) to DNA or its chemical cousin RNA and to other proteins, acting as a docking station for these molecules so they can interact. (medlineplus.gov)
By bringing certain proteins together with DNA or RNA, the hnRNP K protein helps control the activity of genes and the production of proteins. (medlineplus.gov)
By regulating certain genes and proteins, the hnRNP K protein plays a role in the normal development and function of several body systems, including the brain. (medlineplus.gov)
Phenotypes2
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. (yeastgenome.org)
We further test the hypothesis that among these expressed loci, plastically spliced genes are likely to experience the strongest purifying selection to maintain seasonally plastic phenotypes. (lu.se)
Epigenetic3
Michelle then joined the Victor Chang Cardiac Research Institute as a post-doctoral researcher to study epigenetic control of gene regulation in the laboratory of Professor Catherine Suter before taking up an RCUK Academic Fellowship in Professor Vardhman Rakyan's laboratory, Blizard Institute, London, UK. (kcl.ac.uk)
As a post-doctoral researcher, she expanded her interest in the role of epigenetic mechanisms as mediators of gene-environment interactions. (kcl.ac.uk)
Using a novel mismatch-siRNA tool, which precisely distinguishes virus-derived (primary) from target-generated (secondary) siRNAs, we demonstrated that the lack of secondary siRNA production/amplification was responsible for inefficient ViTGS at 29°C. Moreover, inefficient ViTGS at 29°C inhibited the transmission of epigenetic gene silencing to the subsequent generations. (ed.ac.uk)
Sets of genes1
This work identified sets of genes in leaves, stems and roots that were influenced by salt and drought stress treatments. (usda.gov)
Transcriptome1
Pan-transcriptome identifying master genes and regulation network in response to drought and salt stresses in alfalfa (Medicago sativa L. (usda.gov)
Pathways1
The non-specific lethal complex regulates genes and pathways genetically linked to Parkinson's disease. (medscape.com)
Mutations that cause1
HNRNPK gene mutations that cause Au-Kline syndrome result in the production of little or no hnRNP K protein. (medlineplus.gov)
MiRNA mediated1
6/2013 School Award - Joerg Braun, a postdoctoral associate, was awarded the Otto Hahn Medal for his outstanding PhD work on the mechanisms of miRNA-mediated gene silencing from the Max Planck Institute. (umassmed.edu)
Genomic1
Genomic actions involve the regulation of target genes, and suppress most of the vascular elements of inflammation and angiogenesis in the airway. (ersjournals.com)
Protein4
As a proof of concept, we show butyrate-mediated inhibition in binding of HuR to the 3'UTR of COX-2 mRNA resulting in reduced mRNA and protein levels of the inflammatory gene. (metu.edu.tr)
This gene encodes a nuclear matrix protein, which is proposed to stabilize certain messenger RNA species. (nih.gov)
Alternatively spliced transcript variants, including read-through transcripts composed of the upstream small nucleolar RNA host gene 4 (non-protein coding) and matrin 3 gene sequence, have been identified. (nih.gov)
A shortage of this protein alters gene activity and protein production, which disrupts the normal development or functioning of several body systems, resulting in the varied features of Au-Kline syndrome. (medlineplus.gov)
Encodes1
This gene encodes the enzyme responsible for formation of the blood group I antigen. (nih.gov)
Ontology3
according to their Panther/Gene Ontology Classification. (thermofisher.com)
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies ( Molecular Function , Biological Process , and Cellular Component ), a reference, and an evidence code. (yeastgenome.org)
Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes. (yeastgenome.org)
Endogenous1
The vast majority of these cells did not divide, suggesting that the transgene was indeed regulated in a similar fashion as the endogenous GFAP gene. (lu.se)
Tissues2
To inactivate the miRNA processing RNase III gene in the stromal tissues during kidney development we crossed the (allele (Body 1A). (immune-source.com)
These controls are critical for the regulation of many genes across human tissues. (wikipedia.org)
Modifications1
RNA modifications have emerged as key gene regulators. (nature.com)
Biological1
In turn, these hypotheses, which provide reasonable explanations for the biological phenomenon, lead to an enhanced understanding of the gene regulatory network. (hindawi.com)
Regulatory1
To investigate a gene regulatory network, an iterative process of four steps is needed. (hindawi.com)
Cell3
Vaccines, cell and gene therapies, antiretroviral drugs, microbicides, and behavioral strategies for the treatment and prevention of HIV infections are also explored. (cshlpress.com)
One may ask to what extent there are similarities and differences in the gene regulation circuits and their dynamics when it comes to important processes like stem cell regulation. (lu.se)
which means one copy of the altered gene in each cell is sufficient to cause the disorder. (medlineplus.gov)
Phenotype1
Mutations in this gene have been associated with adult i blood group phenotype. (nih.gov)
Nuclear2
Ribonucleoproteína nuclear heterogénea que se encuentra en el NÚCLEO CELULAR y en el CITOPLASMA. (bvsalud.org)
Se ha implicado a la ribonucleoproteína nuclear heterogénea K en la regulación de la expresión genética en casi todos los niveles: TRANSCRIPCIÓN GENÉTICA, procesamiento del ARNm (PROCESAMIENTO DEL ARN POSTRANSCRIPCIONAL), transporte del ARNm, estabilidad del ARNm y traducción (TRADUCCIÓN GENÉTICA). (bvsalud.org)
Human1
The use of viral vectors to deliver foreign genes to the brain is highly logical states in the human brain (Eng et al. (lu.se)