Post transcriptional regulation messenger rna localization of specific rnas. Medical search. Frequent questions

Nuclear mRNA transcripts acquire a protein coat composed of cap- and RNA-binding proteins that allow nuclear export and dictate the functional program of cytoplasmic mRNAs ( Moore, 2005 ). (rupress.org)
Thus, GCGs contain proteins involved in translation initiation, translation control, and mRNA decay, which is consistent with their proposed role in the regulation ofs maternal mRNA expression. (rupress.org)
These genes, encoding for RNA binding proteins, contain a highly conserved RNA recognition motif and at least one DAZ repeat encoding for a 24 amino acids sequence able to bind other mRNA binding proteins. (ijbs.com)
This family includes three members, Boule (or Boll ), Dazl (or Dazla ) and DAZ , encoding RNA binding proteins. (ijbs.com)
The localization of DAZ family proteins suggests that they can regulate mRNA translation occurring in the cytoplasm. (ijbs.com)
These proteins have a highly conserved RNA recognition motif (RRM) for binding target mRNAs and at least one characteristic sequence of 24 amino acids, which are termed as DAZ repeats [ 1 ]. (ijbs.com)
Dazl can regulate the expression, transport and localization of target mRNAs of proteins which control the differentiation, growth and maturation of germ cells. (ijbs.com)
They function by binding to target messenger RNA (mRNA) molecules, leading to their degradation or inhibiting their translation into proteins. (bvsalud.org)
Numerous fundamental cellular processes, including virtually every aspect of gene expression and regulation, depend on the cooperation of RNAs and proteins. (fu-berlin.de)
Specific RNAs and proteins associate to build up some of the most complex macromolecular machineries of living cells, such as ribosomes and spliceosomes, which mediate particular steps of gene expression. (fu-berlin.de)
RNAs and proteins can also engage in more transient interactions, for example during co-transcriptional regulation of RNA polymerases or during post-transcriptional regulation of (pre-)mRNA life cycles. (fu-berlin.de)
We investigate the molecular mechanisms, by which RNAs and proteins cooperate to bring about the biological functions of selected RNPs involved in transcription and pre-mRNA splicing. (fu-berlin.de)
These biomarkers are detectable in liquid biopsy, include circulating tumor cells (CTCs), proteins, cell free DNA (cfDNA) and RNA (cfRNA) that recently have acquired interest in cancer for their role in diagnosis and treatment response [ 1 , 3 ]. (scientificarchives.com)
RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)

Some miRNAs are hosted in pri-miRNAs annotated as long non-coding RNAs (lncRNAs) and defined as MIRHGs (for miRNA Host Genes). (mdpi.com)
Circulating RNAs are represented by a broad range of subtypes, such as microRNA (miRNAs), long non coding RNA (lncRNAs), messenger RNAs (mRNAs), transfer RNAs (tRNAs), snRNAs (small nuclear RNAs), snoRNA (small nucleolar RNAs), piwiRNAs (piRNA) and circRNAs (circular RNAs). (scientificarchives.com)
Circulating RNAs are represented by, such as for intercellular RNAs, a broad range of subtypes, mainly represented by microRNA (miRNAs), long non coding RNA (lncRNAs) and messanger RNAs (mRNAs). (scientificarchives.com)
Purpose: GG-H whole transcriptome array analysis suggested involvement of PININ (PNN) in the alternative splicing of multiple long non-coding RNAs (lncRNAs). (molvis.org)
Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
Alternatively spliced lncRNAs were detected with in situ hybridization with variant-specific RNA probes on human cornea sections. (molvis.org)
Conclusions: The data suggest PNN's role in the alternative splicing of a specific subset of lncRNAs might have a significant impact on the corneal epithelium. (molvis.org)

Many eukaryotic precursor messenger RNAs (pre-mRNAs) bear coding regions (exons) interspersed with non-coding intervening sequences (introns). (fu-berlin.de)
Puf3 allows Ccr4-Not to select specific mRNAs from a mixture of molecules. (elifesciences.org)
Interestingly, compared to total mRNAs, polysome mRNAs contained high levels of m 5 C. Furthermore, an integrative analysis indicated a transcript-specific relationship between RNA m 5 C methylation and mRNA translation efficiency. (biomedcentral.com)

Epitranscriptomics, also known as RNA modifications, refers to the study of post-transcriptional modifications of RNA molecules. (encyclopedia.pub)
MicroRNAs (miRNAs) are small non-coding RNA molecules that play critical roles in post-transcriptional gene regulation. (bvsalud.org)

In addition, RNA modifications have been characterized not only in abundant non-coding RNA, such as transfer RNA (tRNA), ribosomal RNA (rRNA), and small nuclear RNA (snRNAs), but also in messenger RNA (mRNA) [ 2 ] . (encyclopedia.pub)
RNP-based processive anti-termination underlies the switching from immediate-early to delayed-early gene expression in other lambdoid phages as well as ribosomal RNA synthesis in Escherichia coli . (fu-berlin.de)
Huang YH, Said N, Loll B, Wahl MC (2019) Structural basis for the function of SuhB as a transcription factor in ribosomal RNA synthesis. (fu-berlin.de)

More than 60% of all RNA modifications are methylated modifications, and m6A is the most abundant chemical modification in eukaryotic messenger RNA (mRNA). (biomedcentral.com)

Given its diverse roles in mRNA metabolism and gene regulation, altered m6A profiles have been linked to various illnesses, including cancers and psychiatric disorders [ 4 ] . (encyclopedia.pub)
We also study selected RNP-remodeling enzymes as drivers of molecular RNP machineries and as mediators of co- and post-transcriptional gene regulation. (fu-berlin.de)

In endometrial cancer only some of these cf-RNAs have been investigated, no studies have been performed on snoRNAs, piRNAs, snRNAs and tRNA, the most analyzed one is represented by cf-miRNA in plasma. (scientificarchives.com)

Folate is an essential B-group vitamin and a key methyl donor with important biological functions including DNA methylation regulation. (biomedcentral.com)
Epigenetics refers to heritable changes in gene expression without changing the nucleotide sequence of the gene, including DNA methylation, histone modifications, and RNA modifications. (beds.ac.uk)

Because maturing oocytes and early embryos lack appreciable transcription, posttranscriptional regulatory processes control their development. (elifesciences.org)
Despite their critical implication in worldwide public healthcare, essential and available resources such as deep transcriptome annotations remain poor, which also limits our understanding of post-transcriptional control small regulatory RNA (sRNA) functions in these bacteria. (frontiersin.org)
Future research will aim at identifying specific inhibitors and/or inducers of UPR regulatory markers as well as expand the list of UPR-related animal models. (molvis.org)
Bacteria transcribe their genomes with the help of multi-subunit RNA polymerases (RNAPs), which comprise two large β and β' subunits that form the active site, two regulatory α subunits and an ω subunit that supports RNAP assembly. (fu-berlin.de)
Pausing and termination can be further modulated by elongation factors, such as N-utilization substances A and G. Some regulatory factors or RNAs can stably insulate RNAP from the destabilizing effects of terminators over long distances (processive anti-termination). (fu-berlin.de)
In these processes, transcript-borne regulatory RNAs alone or in conjunction with protein factors assemble on the surface of RNAP and accompany the enzyme during further transcription by an RNA looping mechanism, rendering the elongation complex resistant to pause and/or termination signals downstream of the original modification site. (fu-berlin.de)
MicroRNAs (miRNAs) are small regulatory non-coding RNAs, resulting from the cleavage of long primary transcripts (pri-miRNAs) in the nucleus by the Microprocessor complex generating precursors (pre-miRNAs) that are then exported to the cytoplasm and processed into mature miRNAs. (mdpi.com)

These findings open an immediate use of selected cf-miRNA as biomarker in liquid biopsy of EC and suggest further investigation of the other cf-RNAs to implement the knowledge in this fascinating field. (scientificarchives.com)
Even though the role of circulating non coding RNAs as miRNA and lncRNA can be easily understood taking into consideration that can be secreted and persist in biofluid in remarkably stable forms, as "free" or associated to exosomes, less is known concerning the role of circulating mRNA, mainly taking into consideration its instability and the presence of RNase, nevertheless its presence is detected in blood. (scientificarchives.com)
The quality and quantity changes of circulating RNAs, mainly miRNA, are broadly investigated and associated with the initiation and progression of cancer [ 5 ]. (scientificarchives.com)

In the present study, we report the characterization of the full coding sequence of rainbow trout PGMRC1 and mPR beta cDNAs, their tissue distribution, their ovarian expression profiles during oogenesis, their hormonal regulation in the full grown ovary and the in situ localization of PGMRC1 mRNA in the ovary. (biomedcentral.com)

Among the hundreds of known RNA modifications, N6-methyladenosine (m6A) is the most abundant mRNA modification in eukaryotes. (encyclopedia.pub)
It is the most abundant RNA modification, with approximately 25% of mammalian messenger RNAs (mRNA) bearing the mark [ 3 ] . (encyclopedia.pub)
The chemical modification of RNA is a newly discovered epigenetic regulation mechanism in cells and plays a crucial role in a variety of biological processes. (biomedcentral.com)
N6-methyladenine (m6A) mRNA modification is the most abundant form of posttranscriptional RNA modification in eukaryotes. (biomedcentral.com)
Through the development of m6A RNA sequencing, the relevant molecular mechanism of m6A modification has gradually been revealed. (biomedcentral.com)
It has been found that the effect of m6A modification on RNA metabolism involves processing, nuclear export, translation and even decay. (biomedcentral.com)
Recent studies have found that non-coding RNAs and post transcriptional modification of RNAs have become the active fields of cancer research. (biomedcentral.com)
It also opens a new way of thinking for biomedical scientists, whether RNA modification will be another important factor to regulate the biological development of neoplastic diseases. (biomedcentral.com)
In this review, we provide a comprehensive introduction of the latest research progress on m6A modification and elucidate the origin of m6A modification, its regulation, biological functions and its correlation with the central nervous system and gliomas. (biomedcentral.com)
Here, we provide transcriptome-wide landscapes of m 5 C modification in poly(A)-enriched RNAs together with mRNA transcription and translation profiles for mouse neural stem cells (NSCs) cultured in three different concentrations of folate. (biomedcentral.com)
M7G modification, known as one of the common post-transcriptional modifications of RNA, is present in many different types of RNAs. (beds.ac.uk)
With the rapid development of sequencing technology, m7G has become a new research hotspot of RNA modification. (beds.ac.uk)
High-throughput sequencing refers to the use of antibody immunoprecipitation or chemical methods to accurately locate the site of m7G modification in RNA. (beds.ac.uk)

We review the relationship between different classes of these granules and discuss how spatial organization regulates messenger RNA translation/decay. (rupress.org)

With the accurate identification of m7G modifications within RNAs, their functional roles in the regulation of gene expression and different physiological functions have been revealed. (beds.ac.uk)

Different classes of RNA granules share some protein components and may use similar mechanisms to regulate mRNA translation/decay. (rupress.org)

A better understanding of how these species cause disease and spread antibiotic resistance requires a knowledge of how its genes are controlled, on both the DNA and the RNA level. (frontiersin.org)

Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP. (eurekaselect.com)
Regulation of microRNA function in animals. (eurekaselect.com)

Although chemical modifications of RNA have been described for a half-century, only recently with the advancement of technology have we started to elucidate their functions [ 1 ] . (encyclopedia.pub)
To date, over 170 post-transcriptional base modifications have been identified. (encyclopedia.pub)
The post-transcriptional modifications of mRNA, which include N6-methyladenosine (m6A), N1-methyladenosine (m1A), 5-methylcytidine (m5C), N7-methylguanosine (m7G), and N6,2-O-dimethyladenosine (m6Am) add a new layer to regulating mRNA metabolism and gene expression [ 2 ] . (encyclopedia.pub)
Molecular formula of common RNA modifications. (beds.ac.uk)
The detection of RNA modifications is fundamental to the study of m7G modifications in the regulation of gene expression. (beds.ac.uk)

The interacting complex contains the RNA binding motif protein 15/15B (RBM15/15B), vir-like m6A methyltransferase associated (VIRMA), zinc finger CCCH-type containing 13 (ZC3H13), and HAKAI which support the functioning and positioning of a writer complex. (encyclopedia.pub)

Moreover, RNA-protein complexes (RNPs) provide glimpses at the molecular ancestry of modern cells, which most likely evolved from an RNA-dominated world. (fu-berlin.de)

Not only is m7G involved in the normal physiological metabolism of RNA, but recent research also shows that m7G and related regulators appear to be considerably dysregulated in tumors [ 12 ]. (beds.ac.uk)

As seen with many other bacteria, RNA-seq technology has now become the method of choice for transcriptome analysis in the enterococcus. (frontiersin.org)

In this review, we discuss the available RNA-based cancer immunotherapies targeting the TIME. (biomedcentral.com)
More importantly, we summarize the potential of various RNA-based therapeutics clinically available for cancer treatment. (biomedcentral.com)
RNA-dependent targeting of the TIME, as monotherapy or combined with other evolving therapeutics, might be beneficial for cancer patients' treatment in the near future. (biomedcentral.com)
Malentacchi F, Sorbi F, Cipriani N, Sgromo C, Antonuzzo L, Pillozzi S. Circulating Cell-Free RNA: A New Perspective for Endometrial Cancer. (scientificarchives.com)
Recently, circulating RNAs (cfRNAs) are increasing their importance as biomarkers in liquid biopsy for cancer patients both for tumor characterization and development and for setting a monitoring personalized therapy. (scientificarchives.com)

RBM15/15B mediate the binding of a writer complex to the U enriched region on mRNA and recruit writer complexes to specific sites [ 9 ] . (encyclopedia.pub)

However, recent studies have suggested that BRAFi/MEKi and ERK1/2i resistance can arise through activation of a parallel signalling pathway leading to activation of ERK5, an unusual protein kinase that contains both a kinase domain and a transcriptional transactivation domain. (babraham.ac.uk)
When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)

This comprehensive global TSS mapping atlas provides a valuable resource for RNA biology and gene expression analysis in the Enterococci. (frontiersin.org)

Additionally, the delivery of miRNAs to specific target tissues and cells remains a challenge that needs to be addressed for effective clinical translation. (bvsalud.org)

Like all plus-strand RNA viruses, the SARS-CoV-2 replication process within cells requires de novo production of viral RNA species, including single-strand (ss)RNA and double-strand (ds)RNA that can be sensed by cytosolic pattern recognition receptors (PRRs) subsequently activating antiviral pathways 7 . (nature.com)
Substrate recognition by either RNA or DNA sensors leads to signaling cascades that activate two major branches of the innate immune response, the type I/III IFN response and the inflammatory cytokine response (reviewed in ref. 11 ). (nature.com)

Accumulating research suggests that the tumor immune microenvironment (TIME) plays an essential role in regulation of tumor growth and metastasis. (biomedcentral.com)
Different tumor types can also design their specific microenvironment by encouraging tumor angiogenesis and stimulating peripheral immune tolerance. (biomedcentral.com)
The ratio of pro- to antitumor immune populations in the TIME plays a critical role in the regulation of tumor progression and metastasis. (biomedcentral.com)
During virus infection, the specific immune signals produced from infected cells are important for dictating the recruitment and activation of innate or adaptive immune cells that are required to fight virus infection. (nature.com)
Plus-strand RNA viruses have evolved numerous ways to limit or block these cellular immune pathways. (nature.com)

Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)

Knockdown of PNN in HCET cells led to specific changes in the inclusion of multiple cassette exons as well as in the use of alternative splice sites in RP11-322M19.1 and RP11-18I14.1, resulting in considerable net changes in the ratio between the splice variants. (molvis.org)

Identification of phlebovirus and arenavirus RNA sequences that stall and repress the exoribonuclease XRN1. (nih.gov)

Cytoplasmic RNA granules in germ cells (polar and germinal granules), somatic cells (stress granules and processing bodies), and neurons (neuronal granules) have emerged as important players in the posttranscriptional regulation of gene expression. (rupress.org)
Important recent specific progress was brought to my attention by Dr. Victoria Lunyak , a lab director at the Buck Institute who presented on her recent work at the Cell signaling, inflammation and aging symposium in Las Vegas last week, where I was also a presenter. (anti-agingfirewalls.com)

Cellular 5'-3' mRNA exonuclease Xrn1 controls double-stranded RNA accumulation and anti-viral responses. (nih.gov)

Advancements in RNA-based technology have improved understanding of the complexity and diversity of the TIME and its effects on therapy. (biomedcentral.com)

To further assess the importance of these VOCCs, induction of acrosome reaction in capacitated human sperm was quantitated after incubation with SIZP in presence or absence of pharmacological inhibitors of L or T type specific VOCC. (vegfr-3inhibitor.com)

The α 2 ββ'ω core enzyme cooperates with transcription factors and responds to signals on DNA templates and nascent RNAs to achieve full functionality in vivo . (fu-berlin.de)

In the context of skeletal diseases, such as osteoporosis, osteoarthritis, and bone metastasis, there is growing evidence osteoblastic miRNAs, are involved in the regulation of bone formation and maintenance.Osteoblasts are bone-forming cells responsible for synthesizing and depositing the extracellular matrix, which ultimately mineralizes to form bone tissue. (bvsalud.org)
Building on these observations, we found a specific activation of NF-κB and a block of IRF3 nuclear translocation in SARS-CoV-2 infected cells. (nature.com)

Bentham is offering subject-based scholarly content collections which are tailored to meet specific research needs. (eurekaselect.com)

Anatomy25

Organisms16

Diseases1

Chemicals and Drugs87

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Psychiatry and Psychology1

Phenomena and Processes91

Disciplines and Occupations2

Information Science4

Proteins14

LncRNAs7

MRNAs3

Molecules2

Ribosomal3

Eukaryotic1

Gene regulation2

SnRNAs1

Including DNA methylation2

Regulatory7

MiRNA3

Situ1

Modification13

Regulates1

Roles1

Regulate mRNA translation1

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Modifications5

Motif1

Molecular1

Metabolism1

Bacteria1

Cancer5

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Protein2

Biology1

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Important2

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Signals1

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