• Transient transfection analysis with deleted mutants of a luciferase reporter construct showed that the promoter activity was regulated negatively and positively in the distal and proximal promoter regions, respectively. (eurekamag.com)
  • Mechanistically, specificity protein 1 (Sp1), a major transcription factor that controls expression of lens-specific genes such as β-crystallins, was positively regulated by SUMO1 but negatively regulated by SUMO2. (nebraska.edu)
  • SUMO2 also interfered with the interaction between Sp1 and the coactivator, p300, and recruited a repressor, Sp3 to β-crystallin gene promoters, to negatively regulate their expression. (nebraska.edu)
  • cNANOG expression required transcriptional regulatory elements, which were positively regulated by POU5F3 ( OCT4) and SOX2 and negatively regulated by TP53 in PGCs. (biomedcentral.com)
  • Our published findings demonstrate that the FGFR1 gene is positively regulated by the Sp1 transcriptional regulator and negatively regulated by the transcription factor E2F4. (rosalindfranklin.edu)
  • Lnc408 can recruit transcript factor SP3 to CBY1 promoter to serve as an inhibitor in CBY1 transcription in BCSCs. (nature.com)
  • Gene transcription is mainly regulated by transcription factors (TFs) that bind to specific DNA sequences (called motifs) located in the promoter regions of genes [ 1 ]. (biomedcentral.com)
  • Many transcription factor genes regulate their own promoters and when a gene duplicates during evolution then cross-regulation often occurs. (wikipedia.org)
  • Moreover, transcription factors and their target genes form vast interacting gene regulatory networks which can be exquisitely stable. (amegroups.org)
  • PGCs express several pluripotency-related TFs such as NANOG, POU5F3 , and SOX2 , and their expression controls transcription of germness-related genes in these cells [ 11 , 29 ]. (biomedcentral.com)
  • The mechanisms that regulate myoblast cell proliferation versus differentiation involve extracellular signaling via growth factors, their cognate receptors, intracellular signal transduction cascades, and transcriptional regulation of genes associated with cell proliferation. (rosalindfranklin.edu)
  • Among these genes is the fibroblast growth factor receptor 1 (FGFR1) gene which is expressed in proliferating myoblasts and down-regulated during differentiation. (rosalindfranklin.edu)
  • Regulation of the NANOG gene by TFs, epigenetic factors, and autoregulatory factors is well characterized in ESCs, and transcriptional regulation of NANOG is well established in these cells. (biomedcentral.com)
  • Id1 is a member of the helix-loop-helix (HLH) family of transcriptional repressors that act as dominant-negative inhibitors of basic-HLH factors. (oncotarget.com)
  • Id proteins belong to the helix-loop-helix (HLH) family of transcriptional regulators, acting as dominant negative repressors of basic-HLH (bHLH) transcription factors [ 11 - 13 ]. (oncotarget.com)
  • In shortly, these findings highlight that the novel lnc408 functions as an oncogenic factor by recruiting SP3 to inhibit CBY1 expression and β-catenin accumulation in nucleus to maintain stemness properties of BCSCs. (nature.com)
  • KLFs share the similar function of transcription regulation via the recruitment of regulatory proteins. (wikipedia.org)
  • Such cross-regulation occurs extensively in transcription factor families. (wikipedia.org)
  • Interestingly, real-time PCR and translation inhibition studies revealed that survivin was over-expressed partially through the up-regulation of protein translation instead of gene transcription in A549 cancer cells. (biomedcentral.com)
  • This signaling culminates in regulation of transcription factors governing expression of the slow myosin heavy chain 2 gene - indicative of the slow muscle fiber phenotype. (rosalindfranklin.edu)
  • Previous studies revealed that COX-2 is overexpressed in many gliomas and expression level, in particular, is positively correlated with tumor grade [ 9 , 10 ]. (oncotarget.com)
  • We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)
  • We show for the first time that different trans -regulatory elements control transcription of cNANOG in a cell type-specific manner. (biomedcentral.com)
  • From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. (uth.edu)
  • Furthermore, small interfering RNA-mediated knockdown demonstrated that POU5F3 , SOX2 , and CEBP played a role in cell type-specific transcription of cNANOG . (biomedcentral.com)
  • Mechanistically, Id proteins dimerize with bHLH factors to repress their functions. (oncotarget.com)
  • Real-time Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) analysis of EcGS1b gene also showed higher expression in the roots of E.crassipes than in stems and leaves. (biomedcentral.com)
  • Some cells were positively labeled by a expression of a barcoded viral transgene to help establish clonality (marked by an SK). (uth.edu)
  • Cell-to-cell signalling is induced by specific ligands such as growth factors which activate their cognate receptor molecules. (amegroups.org)
  • This family has been expanded to also include the Sp transcription factor and related proteins, forming the Sp/KLF family. (wikipedia.org)
  • These transcription factors have a conserved structural homology between mammalian species, which allow for similar function due to similar protein interaction motifs at the N-terminal domains. (wikipedia.org)
  • There is some evidence that positively-charged amino acids within the three zinc fingers may contribute towards localizing the protein in the Nucleus. (wikipedia.org)
  • Contributing factors include obesity and a central pattern of fat accumulation with a strong genetic component. (diabetesjournals.org)
  • KLF/Sps are a family of transcription factors that contain three carboxyl-terminal (C-terminal) C2H2-type zinc finger structural motifs that bind to the GC-rich regions in DNA and regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. (wikipedia.org)
  • From the contour maps, some key structural factors responsible for the activity of these two series of PIs are revealed. (fdocument.org)
  • A leading contributing factor to metastasis and treatment resistance is the heterogeneity and plasticity of the cells within tumors 6 . (nature.com)
  • NANOG is a core transcription factor (TF) in embryonic stem cells (ESCs) and primordial germ cells (PGCs). (biomedcentral.com)
  • This factor has been shown to be regulated by COX-2 in breast carcinoma cells and recent studies suggest that Id1 may also be involved in the genesis/progression of gliomas. (oncotarget.com)
  • Competitive gel shift and supershift assays indicated that Sp2 and Sp3 were bound to an Sp1 site (-40/-31), and activating transcription factor-2 and c-Jun were bound to a CRE site. (eurekamag.com)
  • We first identified the transcription start site of cNANOG by 5′-rapid amplification of cDNA ends PCR analysis. (biomedcentral.com)
  • Together, our results establish distinct roles of different SUMO isoforms and demonstrate for the first time, to our knowledge, that Sp1 acts as a major transcription factor target for SUMO control of cell differentiation. (nebraska.edu)
  • In addition, resistin mRNA was positively and independently correlated with insulin resistance and hepatic fat as measured by liver X-ray attenuation. (diabetesjournals.org)
  • KLF1 is a very important factor in red blood cell biology. (wikipedia.org)
  • COX-2 is also regulated by epidermal growth factor receptor signaling which is important in the pathogenesis of GBMs. (oncotarget.com)