Positive by microscopy. Medical search. Frequent questions

Sputum Smear Microscopy at Two Months into Continuation-Phase: Should It Be Done in All Patients with Sputum Smear-Positive Tuberculosis? (cdc.gov)
Direct sputum smear microscopy is the most widely used means for the diagnosis of pulmonary tuberculosis (TB) and is available in most primary health-care laboratories at health-centre level. (bvsalud.org)
Smear microscopy may, however, be costly and inconvenient for patients, who have to make multiple visits to health facilities to submit multiple sputum specimens over several days. (bvsalud.org)
As NTM cannot be distinguished from M. tuberculosis through smear microscopy, the presence of NTM can lead to a false-positive diagnosis of tuberculosis. (who.int)
Smear microscopy cannot distinguish between NTM and Mtb . (who.int)

Through combining cryo-electron microscopy at different pHs, X-ray crystallography and biochemical and structural analysis, we showed that NendoU can shift between open and closed forms, which probably correspond to active and inactive states, respectively. (lu.se)

A case with positive immunofluorescence microscopy findings. (medscape.com)
Sagittal sections 0.7 mm from midline were assessed using immunofluorescence microscopy. (cdc.gov)

Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. (wikipedia.org)
Phase-contrast microscopy is particularly important in biology. (wikipedia.org)
After its invention in the early 1930s, phase-contrast microscopy proved to be such an advancement in microscopy that its inventor Frits Zernike was awarded the Nobel Prize in Physics in 1953. (wikipedia.org)
The basic principle to make phase changes visible in phase-contrast microscopy is to separate the illuminating (background) light from the specimen-scattered light (which makes up the foreground details) and to manipulate these differently. (wikipedia.org)
This interactive tutorial explores relationships between the surround ( S ), diffracted ( D ), and resulting particle ( P ) waves in brightfield as well as positive and negative phase contrast microscopy. (microscopyu.com)

Phase-contrast bacterioscopic diagnosis of tuberculosis is spe cific, makes it possible to demonstrate positive results in an additional 26-32 percent of cases and cuts the time by two-three times. (dtic.mil)

Immunofluorescence and immunohistochemistry analyses were performed, and the immunostained sections were analyzed using confocal microscopy. (news-medical.net)
In addition, biofilms were grown in microscope coverslips treated as described above, followed by staining with Propidium Iodide and Fluoresceine for visualization with a confocal laser scanning microscopy. (bvsalud.org)
The confocal laser scanning microscopy analysis demonstrated that all mouthwashes, except for cetylpyridinium chloride, were able to cause membrane damage after 60 s of incubation with S. gordonii biofilms. (bvsalud.org)

In 1941, immunologic procedures were first used in electron microscopic studies of tobacco mosaic virus ( 9 ), and electron microscopy was introduced successfully in the differential diagnosis of smallpox and chickenpox infections in the late 1940s ( 10 , 11 ). (cdc.gov)
In order to characterize more fully and assess its possible functions, 15 samples of surgical and autopsy human thyroid tissue were studied by classical histology, immunohistochemistry, transmission electron microscopy, electron microscopic immunohistochemistry, and scanning electron microscopy. (nih.gov)

Upon graduation, Dr. Kopek moved to the HHMI-Janelia Research Campus in Virginia where he developed new methods for correlative super-resolution fluorescence and electron microscopy. (hope.edu)
Emerging methods in multifrequency atomic force microscopy (MF-AFM) rely on the detection and excitation of higher order eigenmodes of a microcantilever [1-3] and as such, present a number of practical challenges to cantilever instrumentation. (beilstein-journals.org)
Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems. (ki.se)

With the growing use of polarizing plastic containers in cell biology, DIC microscopy is increasingly replaced by Hoffman modulation contrast microscopy, invented by Robert Hoffman in 1975. (wikipedia.org)

Parameters such as staining time and blotting technique affect the proportions between negative and positive staining on the grid and may need to be optimized for each sample. (ki.se)
Negative and Positive Staining in Transmission Electron Microscopy for Virus Diagnosis. (ki.se)

The main constituent of the human thyroid stromal tissue was a CD34 positive reticular network involving fibroblasts, mononuclear cells and nerve terminals. (nih.gov)

This eLearning course is designed to familiarize laboratorians with routine microscopy procedures used in the microbiology laboratory. (cdc.gov)

In 1952, Georges Nomarski patented what is today known as differential interference contrast (DIC) microscopy. (wikipedia.org)
After a simple and fast negative stain preparation, the undirected, "open view" of electron microscopy allows rapid morphologic identification and differential diagnosis of different agents contained in the specimen. (cdc.gov)
Oocysts can also be detected using light microscopy of wet-mounts using differential interference contrast (DIC) microscopy (to provide structural details) or using UV fluorescent microscopy. (asm.org)

Polarised light microscopy of synovial fluid is an established diagnostic technique widely regarded as reliable for the detection of crystals. (unboundmedicine.com)
AU - Gordon,C, AU - Swan,A, AU - Dieppe,P, PY - 1989/9/1/pubmed PY - 1989/9/1/medline PY - 1989/9/1/entrez SP - 737 EP - 42 JF - Annals of the rheumatic diseases JO - Ann Rheum Dis VL - 48 IS - 9 N2 - Polarised light microscopy of synovial fluid is an established diagnostic technique widely regarded as reliable for the detection of crystals. (unboundmedicine.com)
Diagnostic electron microscopy has two advantages over enzyme-linked immunosorbent assay and nucleic acid amplification tests. (cdc.gov)
Applications of diagnostic electron microscopy in clinically or epidemiologically critical situations as well as in bioterrorist events are discussed. (cdc.gov)
To exploit the potential of diagnostic electron microscopy fully, it should be quality controlled, applied as a frontline method, and be coordinated and run in parallel with other diagnostic techniques. (cdc.gov)
Diagnostic electron microscopy reveals coccobacillary bodies that represent the T whipplei organism. (medscape.com)
This is diagnostic because a positive polymerase chain reaction (PCR) for T whipplei will be present in the affected tissue. (medscape.com)
It was found that there was sufficient generalizable evidence that a same-day diagnosis approach is equivalent, in terms of diagnostic accuracy, to conventional microscopy case-finding strategies. (bvsalud.org)

Recently, some specific Gram-positive bacteria, including Mycobacterium tuberculosis and Corynebacterium glutamicum , were found to possess a novel cell wall structure consisting of an arabinogalactan layer covalently linked to the peptidoglycan layer, and a special outer membrane consisting of mycolic acids (mycomembrane) [ 19 ]. (nature.com)
To determine the proportion of non-tuberculous mycobacteria (NTM) in samples of pulmonary tuberculosis (TB) cases from Papua New Guinea who were diagnosed using acid-fast microscopy. (who.int)

The "gold standard" of detection, evaluation of Giemsa-stained blood smears via bright-field microscopy, often requires skilled technicians and laboratory environments that are few and far between in the regions that are most in need. (medgadget.com)

Depending upon the configuration and properties of the phase ring positioned in the objective rear focal plane, specimens can be observed either in positive or negative phase contrast. (microscopyu.com)
UV microscopy of concentrated stool wet-mounts is recommended for routine identification of Cyclospora in clinical specimens. (asm.org)
In addition, lung specimens were obtained from SARS-CoV-2-positive individuals. (news-medical.net)
It has been shown conclusively that good quality microscopy of two consecutive sputum specimens identifies the vast majority (95-98%) of smear-positive TB patients. (bvsalud.org)

Gordon C, Swan A, Dieppe P. Detection of crystals in synovial fluids by light microscopy: sensitivity and reliability. (unboundmedicine.com)
TY - JOUR T1 - Detection of crystals in synovial fluids by light microscopy: sensitivity and reliability. (unboundmedicine.com)
Even when available for field-testing, white light microscopy tends to report many false positive diagnoses as well. (medgadget.com)
Bench-top polarized light microscopy systems, while more definitive, are large, expensive pieces of equipment that are complex to operate, and to maintain as well. (medgadget.com)
ABSTRACT We compared light microscopy examination and a semi-nested multiplex PCR (SnM-PCR) assay in endemic areas of the Islamic Republic of Iran. (who.int)
After 30 minutes, progressive sperm motility was assessed by light microscopy. (nih.gov)
These macrophages can be easily observed infiltrating the tissues using conventional light microscopy. (medscape.com)

In forensic examinations where topographic characterization is important like in fingermark, textile and document forgery examinations, the atomic force microscopy (AFM) imaging technique can be of value. (intechopen.com)
For applications in multifrequency atomic force microscopy (MF-AFM), we illustrate that a single piezoelectric layer can be simultaneously used for multimode excitation and detection of the cantilever deflection. (beilstein-journals.org)

We carefully select, test, and assemble microscopy equipment from a variety of manufacturers to bring you our huge selection of good quality microscopes and microscope accessories. (bargainmicroscopes.com)
Our microscope technicians and sales members take pride in making sure you get the best microscope for your specific microscopy application at a bargain price your budget can afford. (bargainmicroscopes.com)

Inside fingers hold ID slip which becomes embedded in the block for positive permanent identification. (thomassci.com)

Electron microscopy can be applied to many body samples and can also hasten routine cell culture diagnosis. (cdc.gov)

During this current outbreak, a significant number of positive samples identified in commercial laboratories have been readily identifiable with UV microscopy but barely detectable or entirely missed by MAF. (asm.org)

however, UV microscopy is significantly more reliable for detection of the oocysts and should be performed when possible. (asm.org)

MVs are released from the outer membrane of bacterial cells, such as outer membrane vesicles (OMVs) from Gram-negative bacteria, and from the cytoplasmic membrane of cells, such as MVs from Gram-positive bacteria. (nature.com)

Microscopical examination showed that 48.5% (33.8% P. vivax and 14.7% P. falciparum ) and 50% (35.3% P. vivax and 14.7% P. falciparum ) of the samples were positive in 100 and 200 fields respectively. (who.int)
Diverse Protocols for Correlative Super-Resolution Fluorescence imaging and electron microscopy of chemically fixed samples (with M. G. Paez-Segala et al. (hope.edu)
Semen samples of 12 patients were placed in positive control (HTF), negative control (10% K-Y Jelly lubricant), and 10% vol/vol Pre-Seed and FemGlide lubricants. (nih.gov)
At one reference laboratory, despite extensive experience with O&P and MAF, less than 20% of samples that were readily identified as Cyclospora -positive by UV microscopy wet-mount were readily identified by MAF (M. Couturier, unpublished data). (asm.org)
17.56% of KOH-negative samples turned out to be culture-positive (fungal). (bvsalud.org)

SnM-PCR showed the same results as the 200 field microscopy. (who.int)
RESULTS--Twenty-four of the 204 patients (12%) were culture positive for Trichomonas vaginalis and only three of these were wet mount positive. (bmj.com)
Subsequently, all 4 organ donor recipients were tested and had positive results for West Nile Virus RNA. (cdc.gov)
These results may be highly clinically significant, because in the United States alone, f10,000 women are diagnosed annually with ERa-negative/ERh-positive breast carcinoma and may benefit from adjuvant tamoxifen. (lu.se)
13-15 Exposure to NTM has been suggested to impact on the efficacy of the Bacille Calmette-Guérin vaccine 16 and to exhibit cross-reactivity to the tuberculin skin test (TST), leading to increased difficulties in interpreting TST-positive results and evaluating the protection through the only available vaccine against TB. (who.int)

This eLearning course familiarizes laboratory professionals with how to read a Gram stain, colonial characteristics, and biochemical tests used to identify Gram positive microorganisms as well as commonly used testing algorithms. (cdc.gov)

For some cells, FcCH2OH+ penetrated the cell membrane and was reduced, leading to an enhanced current known as positive feedback. (nist.gov)
Cells exhibiting both positive and negative feedback fluoresced green (live) upon exposure to calcein am, which indicates esterase activity in living cells. (nist.gov)
UV microscopy is more reliable than MAF due to the fact that all oocysts will naturally fluoresce, which mitigates the loss of sensitivity that can occur with a high abundance of "ghost cells" on MAF. (asm.org)
these cells were positive for West Nile Virus by RT-PCR, immunofluorescence, and electron microscopy. (cdc.gov)
SARS-CoV-2 spike (S) expression was sparse in cortical microtubule-associated protein 2 (MAP2)-positive and HuC/D-positive neurons and the nestin-positive and Hopx (homeodomain-only protein)-positive radial glial, and Sox2 (sex-determining region Y-box 2)-positive ventricular zone and subventricular zone progenitor cells. (news-medical.net)
En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)

In order to operate the tutorial, use the mouse cursor to translate the Phase Contrast Mode slider between Positive and Negative phase contrast or Brightfield illumination. (microscopyu.com)
The phase plate configurations, wave relationships, and vector diagrams associated with the generation of positive and negative phase contrast images are presented in Figure 1 . (microscopyu.com)

Here, we used a Gram-positive strain, Dietzia sp. (nature.com)
Empiric antibiotic therapy can be narrowly targeted at gram-positive cocci in acute infections. (medscape.com)
Whipple disease is a systemic disease most likely caused by a gram-positive bacterium, Tropheryma whipplei (formerly T whippelii ). (medscape.com)
The Gram positive bacteria Streptococcus mutans are a substantial part of the oral microbiota and their importance in the dental caries etiology is unquestionable 3 . (bvsalud.org)

Through the years of selling microscopes, we have found only a few customers who did not want a bargain, but every customer wanted good quality optical microscopy equipment. (bargainmicroscopes.com)
You have came to the right place to save lots of money on high quality microscopy items. (bargainmicroscopes.com)

High-resolution transmission electron microscopy (HRTEM) correlated the dispersion of the nanofillers and morphology of the nanocomposites. (amrita.edu)
Finally, oxA/hcrt-1-mediated excitatory synaptic transmission onto VTA neurons was not potentiated following an arousing, aversive stimulus, suggesting that oxA/hcrt-1-mediated glutamatergic synaptic transmission was potentiated selectively with highly salient positive reinforcers. (jneurosci.org)

Purpose: Endocrine therapies, such as tamoxifen, are commonly given to most patients with estrogen receptor (ERa) ^ positive breast carcinoma but are not indicated for persons with ERa-negative cancer. (lu.se)

Mixed infections as well as false-positive TB diagnosis cannot be ruled out. (who.int)

An SOP for microscopy tests has to be written before the February 2012. (who.int)

they can change the way electron microscopy is used and can have wide application for multiple techniques for analytical characterization. (2spi.com)

These experiments provide evidence for a selective role of oxA/hcrt-1 signaling in motivation for highly salient reinforcers and may represent a unique opportunity to design novel therapies that selectively reduce excessive drive to consume positive reinforcers of high salience. (jneurosci.org)
All positive phase contrast systems selectively advance the phase of the linear surround (S) wavefront relative to that of the spherical diffracted (D) wavefront. (microscopyu.com)

In the positive phase contrast optical configuration (upper row of images in Figure 1 ), the surround (S) wavefront is advanced in phase by a quarter-wavelength when traversing the phase plate, to produce a net phase shift of 180 degrees (one half wavelength). (microscopyu.com)

Electron Microscopy of Model Systems. (ki.se)

CONCLUSION--The InPouch TV test demonstrated an epidemiological important infected male population that was not indicated by wet mount microscopy. (bmj.com)

False positives were frequent. (unboundmedicine.com)

The flotation method considerably improves the re sults of any type of microscopy. (dtic.mil)

Here, we show that orexin/hypocretin receptor-1 (ox/hcrt-1R) signaling is important for motivation for highly salient, positive reinforcement. (jneurosci.org)

Prevalence of Trichomonas vaginalis in a male sexually transmitted disease clinic population by interview, wet mount microscopy, and the InPouch TV test. (bmj.com)

Anatomy58

Organisms14

Diseases2

Chemicals and Drugs44

Analytical, Diagnostic and Therapeutic Techniques and Equipment78

Phenomena and Processes55

Disciplines and Occupations9

Technology, Industry, Agriculture12

Information Science9

Health Care3

Smear Microscopy5

Cryo-electron m1

Immunofluorescence microscopy2

Phase-contrast m5

Diagnosis of tuberculosis1

Confocal3

Electron microscopic2

Methods3

19751

Staining2

Fibroblasts1

Microbiology1

Differential3

Diagnostic8

Tuberculosis2

Smears1

Specimens4

Light7

Atomic2

Microscope2

Identification1

Routine1

Outbreak1

Detection1

Cytoplasmic1

Samples5

Results5

Biochemical1

Cells6

Negative phase contrast2

Gram4

Quality2

Transmission2

Patients1

Infections1

Tests1

Techniques1

Selectively2

Optical1

Systems1

CONCLUSION1

False1

Type1

Important1

Male1