Population distinct inner cell. Medical search. Frequent questions

Our study provides a conceptual basis for future strategies aiming to create functionally distinct T cell populations for adoptive therapies by selectively tuning mechanical input provided by biomaterials-based engineered cell culture systems. (sciencedaily.com)
These data suggest that CCK interneurons of the BLA are a heterogeneous population and may be functionally distinct subpopulations that differentially contribute to the processing of emotionally salient stimuli. (nih.gov)
Finally, we provide evidence that colonic subepithelial PDGFRα hi fibroblasts comprise several functionally distinct populations that originate from an Fgfr2-expressing fibroblast intermediate. (lu.se)

Cell identities of samples derived from heterogeneous subpopulations are then determined by clustering of scRNA-seq data. (nih.gov)
My lab's work has pursued how multiple discrete subpopulations of malignant cells within these tumors cooperatively resist therapy via both tumor cell autonomous mechanisms and crosstalk with the stromal microenvironment. (upenn.edu)
Fluorochrome-labeled antibodies distinguish populations and subpopulations of WBCs. (cdc.gov)

Methods AF-MSCs, hepatic progenitor-like (HPL) cells and hepatocyte-like (HL) cells derived from AF-MSCs were transplanted into CCl 4 -injured NOD/SCID mice with the AHF phenotype in order to evaluate their therapeutic potential. (bmj.com)
However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
We examine this hypothesis through functional and molecular analysis of individual blood cells captured from self-renewal cultures, during cytokine-driven differentiation and from primary stem and progenitor bone marrow compartments. (lu.se)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

In a recent study published in the Journal of Hepatology , researchers reported that the coronavirus disease 2019 (COVID-19) vaccination could elicit a distinct T cell-dominant immune-mediated hepatitis. (news-medical.net)
A 5.3-fold increase in immune cells was observed relative to control (non-diseased) tissue. (news-medical.net)
The cluster of differentiation 8 (CD8) T lymphocytes was the most abundant among immune cells, a condition unanticipated for AIH. (news-medical.net)
Spatial analysis of liver parenchyma for different subsets of immune cells revealed more extensive immune infiltration in the periportal areas. (news-medical.net)
It consists of 4 fluorescent channels (UV to far red) and has extensive applications including bright field cell counting, wound-healing and cell growth assays, cell line development, induced pluripotent stem cell (iPSC) reprogramming, 3D invasion and migration modeling, immune-oncology, virology, and fluorescent-based assays. (utsouthwestern.edu)
The successful campaign of adoptive T cell therapies, a type of immunotherapy in which immune T cells are collected from a patient, enhanced outside of the body, and reinfused back into the same patient, especially against blood cancers is well under way. (sciencedaily.com)
Multiple myeloma (MM) is an incurable cancer in which uncontrolled plasma cell proliferation disrupts the bone marrow environment and impairs immune function. (hrb.ie)
Third, peripheral blood mononuclear cells are used to explore heterogeneous immune populations. (nih.gov)
Among immune cells, Foxp3 + regulatory T cells (Treg cells) are potent inhibitors of cancer immunity, and their presence within solid tumors is generally associated with a poor prognosis. (mdpi.com)
We subsequently show how eQTLs have dynamic allelic effects in B cells transitioning from naïve to memory states, and demonstrate how commonly segregating alleles lead to inter-individual variation in immune function. (iscb.org)
Ultimately the virus is able to persist in a latent state in memory B cells such that it is invisible to the immune system and also harmless. (tufts.edu)
Anatomically the CNS and immune systems are quite distinct and different. (directlabs.com)
Accurate and reliable measures of CD4+ T-lymphocytes (CD4+ T-cells) are essential to the assessment of the immune system of human immunodeficiency virus (HIV)-infected persons (1-3). (cdc.gov)
A database search curated for known matrix protease substrates and predicted signaling motifs enabled identification of 73 MWCNT-responsive peptides, which were significantly associated with an abnormal cardiovascular phenotype, extracellular matrix organization, immune-inflammatory processes, cell receptor signaling, and a MWCNT-altered serum exosome population. (cdc.gov)

The cells in the light fraction suppressed the in vitro PFC response to SRBC and DNP-LPS. (aai.org)
This enabled them to demonstrate a distinct impact of tissue viscoelasticity on T cell development and function in vitro and in vivo , and to identify a molecular pathway driving the phenomenon. (sciencedaily.com)
In vitro , CD3 + CD8 + CD27 - CD28 - compared to CD3 + CD8 + CD27 + CD28 + CART cells displayed similar CD19 + target cell-specific cytotoxicity, but were hypoproliferative and produced less cytotoxic cytokines (IFN-γ and TNF-α). (frontiersin.org)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
Finally, EPB41L4A-AS2 inhibited breast cancer cell proliferation, migration and invasion and induced cell apoptosis in vitro. (cancerindex.org)
Analyses of in vitro self-renewing multipotent systems have revealed cellular heterogeneity with individual cells transiently exhibiting distinct biases for lineage commitment 1-6 . (lu.se)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

First, a mixture of Jurkat and 293T cell lines provides two distinct cellular populations. (nih.gov)

Single cell RNA-Seq reveals support cell heterogeneity. (elifesciences.org)
Trajectory analysis of single cell RNA-seq datasets of E12.5 and adult mesenchymal cells suggest that adult smooth muscle cells and fibroblasts derive from distinct embryonic intermediates and that adult fibroblast subsets develop in a linear trajectory from CD81 + fibroblasts. (lu.se)

Single-cell genetic analysis of clonal dynamics in colorectal adenomas indicates CDX2 gain as a predictor of recurrence. (cancerindex.org)
Whereas their clonal populations are genetically identical, they contain phenotypically distinct individuals. (weizmann.ac.il)

Splenic transitional B-cells (T1 and T2) are selected to avoid self-reactivity and to safeguard against autoimmunity, then differentiate into mature follicular (FO-I and FO-II) and marginal zone (MZ) subsets. (miami.edu)
Transcriptomic analysis by RNA-seq of the five B-cell subsets revealed T1 cell signature genes included RAG suggesting a potential for receptor revision. (miami.edu)
Together, these findings suggest, developmental plasticity among splenic B-cell subsets, potential for receptor revision in peripheral tolerance whereas enhanced metabolism coincides with T2 to mature B-cell differentiation. (miami.edu)
This includes the expression of distinct antigens on subsets of MSCs, which was utilized recently to define and separate functionally different subsets from bulk MSCs. (hindawi.com)
We therefore briefly discuss differences found in subsets of human bmMSCs and in MSCs isolated from some other sources and touch upon how this could be utilized for cell-based therapies. (hindawi.com)
We perform in vivo transplantation and lineage-tracing experiments to demonstrate that adult intestinal fibroblast subsets, smooth muscle cells and pericytes derive from Gli1-expressing precursors present in embryonic day 12.5 intestine. (lu.se)

nonclassical memory B cells/(nCMCD19)/CD27+IgD+ and classical memory B cells/(CMCD19)/CD27+IgD-.We performed intra-arm analyses by paired t-test to compare memory-related phenotypic features observed in participants with previous vaccination with paired samples collected early after vaccination: NV(d0) versus PV(d30-45) and #PV(y≥10) versus RV(d30-45). (cdc.gov)
In the process, we have developed the ability to analyze heterogeneity and phenotypic plasticity within cancer cell lines, patient-derived xenografts, and primary human cancer samples based on molecular and functional criteria. (upenn.edu)
Yet, the phenotypic landscape in any given species remains largely unexplored due to the technical challenges of systematically profiling individual bacterial cells. (weizmann.ac.il)

Retinal ganglion cells, the sole output neurons of the retina, exhibit surprising diversity. (frontiersin.org)
We evaluate our approach using synthetic spike trains, and demonstrate that major known cell types are identified in high-density recording sessions from the mouse retina with around 1,000 retinal ganglion cells. (frontiersin.org)
It is well established that the retina has multiple, functionally complementary populations of retinal ganglion cells (RGCs), which together transmit visual information to various central visual areas ( Roska and Werblin, 2001 ). (frontiersin.org)
These results suggested that the endogenous neurogenic capacity of retinal Mller glial cells could be improved by this little molecular agonist from the SHH signaling pathway. (exposed-skin-care.net)
Consequently, the present research looked into whether SHH could be changed by purmorphamine within the transdifferentiation of Mller glial cells to retinal neurons, and therefore, attempted to give a far more convenient, stabilized and effective therapy. (exposed-skin-care.net)
Mller glial cell tradition Primary ethnicities of retinal Mller glial cells had been ready as previously referred to (14). (exposed-skin-care.net)

Figure 7: Female VMHvl has anatomically and molecularly distinct subdivisions. (nature.com)

Despite this heterogeneous response, blood pre-infusion biomarkers predicting responsiveness to CART cell therapy are currently understudied. (frontiersin.org)
However, little is known about the physiological properties of CCK interneurons in the BLA and/or whether they represent a homogeneous or heterogeneous population. (nih.gov)
Altogether our FACS (fluorescence-activated cell sorter) analyses reveal that the neonatal subventricular zone is far more heterogeneous than previously suspected and our studies provide new insights into the signals and mechanisms that regulate their self-renewal and proliferation. (karger.com)

Importantly, CD45-AP mutant mice had reduced transitional and increased mature MZ and FO B-cells, suggesting that it prevents premature entry of transitional B-cells to the mature B-cell pool or their survival and proliferation. (miami.edu)
Following 2 days of culture, cells on the coverslips were fixed in 4% paraformaldehyde at 4C for 10 min and processed for immunocytochemistry to detect proliferation-associated markers. (exposed-skin-care.net)
The latter findings point to the participation of Nogo-A/NgR1 signaling in the regulation of other aspects of growth, such as tissue expansion or turnover by cell proliferation. (jneurosci.org)
Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)

Single-cell RNA-sequencing (scRNA-seq) allows us to dissect transcriptional heterogeneity arising from cellular types, spatio-temporal contexts and environmental stimuli. (nih.gov)
We are particularly interested in how single-cell heterogeneity manifests in plant-associated bacteria and opportunistic pathogens. (weizmann.ac.il)

Unlike the development of squamous cell carcinoma, the development of sarcomas is unrelated to smoking and alcohol use. (medscape.com)
Oral HPV infection is newly appreciated as a strong risk factor for a distinct type of oropharyngeal squamous cell carcinoma that is rising in incidences in the United States. (cdc.gov)
Several classes of drug have been approved for the treatment of melanoma, renal cell carcinoma, non-small cell lung cancer, bladder cancer, and head and neck squamous cell carcinoma, among others. (medscape.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
T cells experience different mechanical signals in different tissues. (sciencedaily.com)
Researchers have engineered a tissue-mimicking hydrogel model to show that more elastic tissues induce T cells to become effector-like T cells with strong tumor-killing potential, while more viscous tissues induce them to become memory-like T cells. (sciencedaily.com)
Researchers at Harvard's Wyss Institute and Harvard SEAS led by David Mooney engineered a tissue-mimicking hydrogel model to show that more elastic tissues induce T cells to become effector-like T cells with strong tumor-killing potential, while more viscous tissues induce them to become memory-like T cells. (sciencedaily.com)
One way to approach this goal is to better understand how T cells' traits and functions, including their cytotoxic effects on unwanted target cells (effector T cells) or their ability to recall and eliminate them if they show up again (memory T cells), are shaped by the mechanical resistance of the tissues they encounter while infiltrating them. (sciencedaily.com)
By engineering a 3-dimensional model of the extracellular matrix (ECM), produced by cells that are responsible for tissues' different stiffnesses and viscoelasticities, they were able to tune both parameters independently. (sciencedaily.com)
Importantly, the phenotypes, functions, and gene expression programs of T cells trained in variations of the system correlated well with those we found in T cells in mechanically distinct tissues from patients with cancer or fibrosis," said Mooney who also is the Robert P. Pinkas Family Professor of Bioengineering at SEAS, and leads the Wyss Institute's Immunomaterials Initiative. (sciencedaily.com)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
Our lab applies single-cell and spatial transcriptomics approaches to globally study cell-cell variation across lifestyles, from free-living populations and biofilms assemblies to bacterial colonization of host tissues. (weizmann.ac.il)
Cilia are important for the structure and function of many types of cells and tissues, including cells in the kidneys, liver, brain, and the light-sensitive tissue at the back of the eye ( the retina ). (medlineplus.gov)
In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)

Next, flow cytometry analyzed intrahepatic and peripheral populations of CD8 T cells in depth. (news-medical.net)
The incorporation of a flow cytometer and a cell sorter also reduces the cost associated with recurrent use of the main flow cytometry facility on campus. (utsouthwestern.edu)
Here we define a 4-color flow cytometry panel using CD133, LeX, CD140a, NG2 to define a neural stem cell (NSC) as well as 4 classes of multipotential progenitors and 3 classes of bipotential progenitors, several of which have not been described previously. (karger.com)
The CDC guidelines concerning CD4+ T-cell determinations (33) were first published in the MMWR in 1992 to provide laboratorians with the most complete information about how to measure CD4+ T-lymphocytes in blood from HIV-infected persons by using flow cytometry. (cdc.gov)

Yet, if different RGC types have distinct cellular physiological properties, light responses, and systematic differences in their presynaptic input neurons, it may be possible to use a sufficiently rich stimulus to evoke responses to unmask their distinctive functional features. (frontiersin.org)
This is consistent with a negative-feedback loop, in which newly generated neurons modulate cell division of SVZ stem cells. (jneurosci.org)

This differentiation of stem cells to a cell type that performs a specific function is thought to be irreversible. (medicalnewstoday.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)

Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)

This glossary of genetics and evolutionary biology is a list of definitions of terms and concepts used in the study of genetics and evolutionary biology, as well as sub-disciplines and related fields, with an emphasis on classical genetics, quantitative genetics, population biology, phylogenetics, speciation, and systematics. (wikipedia.org)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
The proposed research project is strongly multidisciplinary, and involves a combination of polymer chemistry, nanomaterials engineering, cell biology, and imaging techniques. (europa.eu)

Background There is increasing interest in the therapeutic potential of human mesenchymal stem cells (hMSCs), especially in diseases such as acute hepatic failure (AHF) that are predominantly caused by a variety of drugs and viruses. (bmj.com)
Further understand the mechanism of host cells promotion of cancer metastasis with a focus on myeloid cells and mesenchymal stem cells. (ed.ac.uk)

Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)

Brain myeloid cells, particularly microglia, play key roles in these conditions. (elsevierpure.com)
The proteins produced from NPHP1 and the other genes involved in nephronophthisis are known or suspected to play roles in cell structures called cilia. (medlineplus.gov)
In addition, as GPR37L1 expression in the chick glial cells differed from GPR37 expression in mammalian glial cells, the roles of GPR37 and GPR37L1 for prosaposin may differ between birds and mammals. (bvsalud.org)

Further, the team found infiltrates of T and B lymphocytes, macrophages, granulocytes, and plasma cells in the liver. (news-medical.net)
While B and plasma cells were enriched predominantly in the periportal regions, a panlobular distribution was noted for CD8 T lymphocytes. (news-medical.net)
A 3.4-fold enrichment of SARS-CoV-2 S-specific CD8 T lymphocytes was noted in the intrahepatic CD8 T cell population compared to peripheral blood. (news-medical.net)
Moreover, S-specific CD8 T lymphocytes were 10.2-fold more abundant in the peripheral blood than T lymphocytes specific for an Epstein-Barr virus-specific T cell control epitope. (news-medical.net)
and the percentage of lymphocytes that are CD4+ T-cells. (cdc.gov)

Notably, cytotoxic CD8 T cells (characterized by granzyme B) were highly accumulated, while the levels of other granzyme B-expressing cells remained unchanged. (news-medical.net)
Compared to healthy controls (n=24), DLBCL patients (n=33) showed significant lymphopenia, due to low CD3 + CD4 + T helper and CD3 - CD56 + NK cell counts, while cytotoxic CD3 + CD8 + T cell counts were similar. (frontiersin.org)
A Tip leads cytotoxic T cells to the crime scene in neuroinflammation. (stanford.edu)

Blood-derived monocytes comprised five populations and migrated to the brain in EAE, but not in HD and ALS models. (elsevierpure.com)

In summary, we find that ERV expression is a hallmark of cellular identity and cell potency that characterizes the cell populations in early human embryos. (ntu.edu.sg)
The researchers took these X-only (or "XO") cells and injected them into normal mouse embryos, which they then implanted in a surrogate mother mouse. (livescience.com)

The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)

A two-stepped smFISH protocol allows for many genes to be measured sequentially in the same cells by selectively turning fluorescence ON or OFF. (weizmann.ac.il)
NfsB is a dimeric flavoprotein enzyme that has broad electron acceptor specificity 15 and has been used in conjunction with prodrugs to selectively ablate cells in vertebrates. (cdc.gov)

MZ B-cells possessed the most distinct transcriptome including down-regulation of CD45 phosphatase-associated protein (CD45-AP/PTPRC-AP), as well as upregulation of IL-9R and innate molecules TLR3, TLR7, and bactericidal Perforin-2 (MPEG1). (miami.edu)
In haematopoiesis, population-based studies have indicated that lineage choice is governed by global transcriptional noise, with self-renewing multipotent cells reversibly activating transcriptome-wide lineage-affiliated programs 7 . (lu.se)
We show dissociation between self-renewal potential and transcriptome-wide activation of lineage programs, and instead suggest that multipotent cells experience independent activation of individual regulators resulting in a low probability of transition to the committed state. (lu.se)

Conversion of human embryonic stem cells (hESCs) to an epiblast-like state activates blastocyst-specific ERV elements, indicating that their activity dynamically reacts to changes in regulatory networks. (ntu.edu.sg)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)

Further, unique core transcriptional signatures in MZ B-cells may control their innate features. (miami.edu)
Transcriptional Control of Regulatory T Cells in Cancer: Toward Therapeutic Targeting? (mdpi.com)

E ) Table of marker genes that distinguish the different cell clusters. (elifesciences.org)
However, stem cells are difficult to distinguish from multipotential progenitors, which may look and act like them. (karger.com)

Abstract: Epstein-Barr virus (EBV) infects more than 95% of the adult world population. (tufts.edu)

Stem cells rely on extracellular signals produced by the niche, which dictate their ability to self-renew, expand and differentiate. (karger.com)

RNAhello will construct such vesicles from polymers and employ them as mRNA shuttles between distinct protocell populations. (europa.eu)
Previous generations of polymer protocells have been shown to allow diffusion based communication with small molecules and naked DNA strands - the project will expand upon this by investigating polymersomes as mRNA shuttles between distinct protocell populations. (europa.eu)
While GPR37L1 mRNA expression was observed in the oligodendrocytes/Schwann cells in the vestibular ganglion, GPR37 mRNA expression was observed in the crista ampullaris base region. (bvsalud.org)

First, researchers engineered cells from a male mouse fetus - Father No. 1 - to turn the cells into pluripotent stem cells. (livescience.com)
Pluripotent stem cells can become any cell in the body. (livescience.com)
However, when researchers make pluripotent stem cells in the lab, about 1 percent spontaneously lose their Y chromosomes because of natural genetic mistakes. (livescience.com)

CD8 T cells in peripheral blood also expressed CD38. (news-medical.net)
We present single cell RNA sequence data from 1.3m peripheral blood mononuclear cells from 982 healthy human subjects. (iscb.org)
The serum peptide fraction, having cell-surface receptor ligand properties, conveys peripheral bioactivity in promoting endothelial cell inflammation, vasodilatory dysfunction and inhibiting angiogenesis. (cdc.gov)

The intestinal lamina propria contains a diverse network of fibroblasts that provide key support functions to cells within their local environment. (lu.se)

Furthermore, SHH-treated cells had been shifted to neural lineage by expressing neuron-specific course III -tubulin (Tuj1), directing cell destiny to pole Cbz-B3A cells (14). (exposed-skin-care.net)
In the adult mammalian subventricular zone (SVZ), GFAP-positive neural stem cells (NSCs) generate neuroblasts that migrate tangentially along the rostral migratory stream (RMS) toward the olfactory bulb (OB). (jneurosci.org)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

Chimeric antigen receptor T (CART) cell therapy targeting the B cell specific differentiation antigen CD19 has shown clinical efficacy in a subset of relapsed/refractory (r/r) diffuse large B cell lymphoma (DLBCL) patients. (frontiersin.org)

Translocation carcinoma is a renal cell carcinoma (RCC) subtype that harbors a translocation involving a member of the microphthalmia transcription factor gene family. (medscape.com)
In Xp11.2 translocation-associated renal cell carcinoma (RCC), cells typically have voluminous cytoplasm, which can range from eosinophilic and granular to clear. (medscape.com)
In Xp11.2 translocation-associated renal cell carcinoma, tumor cells with abundant eosinophilic cytoplasm and high nuclear grade are often arranged in large nests with a delicate, intervening vascular stroma. (medscape.com)

While molecular subtyping now guides treatment selection for patients with non-small cell lung cancer and other cancers, SCLC is still treated as a single disease entity. (oncotarget.com)
During the third quarter of 2021, we continued to advance our clinical trials of CA-4948, our first-in-class, small molecule IRAK4 inhibitor in nine distinct patient populations across AML, MDS and B cell cancers, including the recent initiation of our Phase 1 combination study in AML/MDS. (biospace.com)
Enrollment remains on track in nine distinct patient populations across AML, MDS and B cell cancers. (biospace.com)

allochronic isolation The isolation of two populations of a species due to a change in breeding periods. (wikipedia.org)
This isolation acts as a precursor to allochronic speciation, a type of speciation which results when two populations of a species become isolated due to differences in reproductive timing. (wikipedia.org)
allopatric speciation A mode of speciation where the evolution of reproductive isolation is caused by the geographic separation of two or more populations of a single species. (wikipedia.org)
allopatry The phenomenon by which two or more populations of a single species exist in geographic isolation from one another. (wikipedia.org)
fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

Recent studies suggest that cells in the ventrolateral part of the ventromedial hypothalamus (VMHvl) that express estrogen receptor-α (Esr1) and progesterone receptor are essential for male but not female mouse aggression. (nature.com)

injection of purmorphamine promoted the activation of Mller glial cells, and in turn, the production of rod-like photoreceptors in acute damaged retina. (exposed-skin-care.net)
Cell transdifferentiation methodology To examine the regenerative potential of Mller glial cells, 1104 cells/ml were plated on poly-D-lysine (500 g/ml) and laminin (5 g/ml) coated glass coverslips. (exposed-skin-care.net)
R&D Systems, Inc.) was added to purmorphamine-stimulated Mller glial cells to determine whether the Wnt pathway was involved. (exposed-skin-care.net)

We combined this with whole cell patch-clamp recording techniques to examine the physiological properties of CCK-containing interneurons of the rat BLA. (nih.gov)

central memory T cells/(CMCD4;CMCD8)/CD27+CD45RO+ and effector memory T cells/(EMCD4;EMCD8)/CD27-CD45RO+. (cdc.gov)

2004), the ABC trans- panded neurosphere cells and also mark subfractions porter Bcrp1/ABCG2 (Hulspas et al. (lu.se)

In spite of such accumulating knowledge of Rac1 and Cdc42 downstream pathways, little is known about the functional differences between Rac1 and Cdc42, in particular, how these Rho GTPases participate in cell migration. (nature.com)
Single-cell analysis resolved differences in signaling and cytokine production within similar myeloid populations in EAE compared to HD and ALS models. (elsevierpure.com)

Blocking studies of IL-10 secretion from HPL cells confirmed the therapeutic significance of this cytokine in the AHF mouse model. (bmj.com)

Furthermore, imaging of intact lungs revealed that macrophages are required for efficient tumor cell extravasation. (elsevierpure.com)
Conclusion/Significance: These data indicate a direct enhancement of metastatic growth by macrophages through their effects on tumor cell extravasation, survival and subsequent growth and identifies these cells as a new therapeutic target for treatment of metastatic disease. (elsevierpure.com)

This new concept could help advance adaptive T cell therapies by producing desired patient-specific T cell populations in the dish that could provide stronger effects when infused back into the same patient. (sciencedaily.com)
But improving the ability to create patient-specific T cell populations with specific traits and functions could broaden clinicians' repertoire of T cell therapies. (sciencedaily.com)
Thus, understanding how Treg cell identity is controlled, is of utmost importance for the development of novel anti-cancer therapies. (mdpi.com)

Using gene-deleted and overexpressing transgenic mice we show that IL-9/IL-9R interaction resulted in rapid activation of STAT1, 3, and 5, primarily in MZ B-cells. (miami.edu)
For 14 cell types, we identified 26,597 independent cis-expression quantitative trait loci (eQTLs), and 62,305 trans-eQTL, with the majority showing cell type specific effects on gene expression. (iscb.org)
which means both copies of the gene in each cell have mutations. (medlineplus.gov)
11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)
Finally, we constructed a Tva gene-edited cell line with L55R and W69L substitutions that could fully resist ALV-A entry, while Cbl uptake was not affected. (bvsalud.org)
Additionally, we constructed gene-edited cell lines targeting these two amino acids, which completely restricted ALV-A entry without affecting Cbl uptake. (bvsalud.org)
Other factors have been suggested to correlate with response to immunotherapy based on clinical and preclinical studies, including elevated type 1 helper T cell (Th1) tumoral gene signature, [ 11 ] and IFN-γ expression, possibly attributable to its ties to other proteins and cytokines within the tumor microenvironment. (medscape.com)

Our results revealed that PBS degradation and re-synthesis comprise a rapid response to nitrogen fluctuations, with coordinated populations of cells undergoing pigment modifications. (osti.gov)
Hair follicles contain several different types of cells, including melanocyte stem cells that generate cells that produce the pigment melanin responsible for hair color. (medicalnewstoday.com)
A new study in mice shows that these melanocyte stem cells migrate between two sites in the hair follicle during each cycle of hair growth and shedding- from a site where they produce the pigment for hair color to another where they produce stem cells. (medicalnewstoday.com)
The increase in the number of follicle growth cycles with aging is associated with deficits in melanocyte stem cells (McSCs) , the stem cells residing in the follicle that can form hair pigment-producing melanocytes. (medicalnewstoday.com)
Instead, a recent study published in Nature suggests that the McSCs can migrate back and forth between the two aforementioned locations, differentiating to produce hair pigment-producing melanocytes in the hair germ area and then translocating to the bulge and dedifferentiating to ensure the maintenance of an adequate supply of stem cells. (medicalnewstoday.com)
This results in fewer stem cells that can develop into pigment-producing melanocytes, thus leading to hair graying. (medicalnewstoday.com)

at least focally, tumor cells often have abundant clear cytoplasm, mimicking clear cell RCC. (medscape.com)

PCC 6803 cells using hyperspectral confocal fluorescence microscopy and multivariate image analysis. (osti.gov)
C) Cells are then imaged together and fluorescence reports the condition from which they were taken. (weizmann.ac.il)
The fluorochrome-labeled cells are analyzed by using a flow cytometer, which categorizes individual cells according to size, granularity, fluorochrome, and intensity of fluorescence. (cdc.gov)

MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
First, with a profiling study, I identified a unique pattern of viral miRNA expression by normal persistently infected primary B cells in vivo and a subset of miRNAs that might be associated with cell growth and are deregulated in tumors. (tufts.edu)
The peptide fraction was then found bioactive, producing endothelial cell inflammation and vascular dysfunction ex vivo akin to that induced with whole serum. (cdc.gov)
The "In vivo Brain Circuit Analysis" platform is a research infrastructure devoted to integrating methods and techniques to interrogate neuronal circuits in health and disease using advanced methods for the perturbation and recording of cell activity. (lu.se)

The suppressor cells found in the spleens of mice bearing advanced tumors could be divided into two different populations by centrifugation on Hypaque-Ficoll of specific gravity 1.08. (aai.org)
Figure 8: VMHpvlm and VMHpvll in female mice have distinct downstream targets. (nature.com)
In a first for stem-cell technology, researchers have produced mice with two fathers. (livescience.com)
The resulting baby mice were chimeras , or organisms with several distinct populations of cells. (livescience.com)
About half of the mice were female chimeras with some XX cells (which came from the normal embryo) and some XO cells, which contained DNA from Father No. 1. (livescience.com)
To do so without the multigenerational approach used to produce the mice, scientists would have to coax male cell lines to generate egg cells. (livescience.com)

As a result, rapid compensation to targeting these pathways occurs not only at the level of signaling networks in individual cells but also through dynamics among diverse cell states cooperatively sustaining cancer progression. (upenn.edu)
In this review, we summarize the current knowledge on the different intracellular pathways involved in the programming of Treg cell homeostasis and functions in cancer. (mdpi.com)

They appeared to be of the macrophage/monocyte line since they were adherent to plastic and nylon wool but were not removed by anti-BA-ϑ serum plus complement (C). They did not require DNA synthesis for activity since they were not inactivated by mitomycin C. The cells in the dense fraction suppressed only the response to SRBC. (aai.org)
They may have been T cells since they were not adherent to plastic or nylon wool and their activity was reduced by treatment with anti-BA-ϑ serum plus C. They did require DNA synthesis since their activity was removed by treatment with mitomycin C. Suppression of normal lymphocyte function could be affected when splenic suppressor cells were separated from the normal population by a cell-impermeable membrane. (aai.org)
Blood cell and serum markers, along with clinical data of DLBCL patients who were scheduled for CART cell therapy were evaluated to search for biomarkers predicting CART cell responsiveness. (frontiersin.org)
The clinical characteristics were: pubertal onset, female preponderance, obesity, strong family history of type 2 diabetes mellitus, high plasma glucose at presentation, adequate cell reserve and serum pancreatic islet cell antibody negativity. (who.int)

Anatomy58

Organisms13

Diseases8

Chemicals and Drugs46

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Psychiatry and Psychology1

Phenomena and Processes57

Disciplines and Occupations2

Information Science3

Functionally distinct3

Subpopulations3

Progenitor6

Immune14

Vitro9

Cellular populations1

Single cell RNA-Seq2

Clonal2

Subsets6

Phenotypic3

Retinal6

Anatomically1

Heterogeneous3

Proliferation4

Heterogeneity2

Squamous3

Tissues11

Flow cytometry4

Neurons2

Differentiation of stem cells2

Murine1

Biology3

Mesenchymal2

Hematopoietic1

Roles3

Lymphocytes5

Cytotoxic3

Monocytes1

Embryos2

Bone marrow2

Selectively2

Transcriptome3

Embryonic3

Transcriptional2

Distinguish2

Abstract1

Extracellular1

MRNA3

Pluripotent stem3

Peripheral3

Fibroblasts1

Neural3

Antigen receptor1

Renal cell carc3

Cancers3

Isolation5

Receptor1

Glial cells3

Physiological properties1

Effector1

Neurosphere cells1

Differences2

Cytokine1

Tumor cell2

Therapies3

Gene7

Pigment6

Least focally1

Fluorescence3

Vivo4

Mice6

Pathways2

Serum4