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  • Substrate
  • In an effort to understand the potential role that two putative alginate lyases (Smlt1473 and Smlt2602) from S. maltophilia may play in bacterial virulence, each enzyme was heterologously expressed in Escherichia coli, purified in a one-step fashion via affinity chromatography, and assayed for catalytic activity as well as substrate specificity for a range of polysaccharides. (lehigh.edu)
  • Altogether, this work furthers our understanding of S. maltophlia, an emergent, multidrug resistant opportunistic pathogen that is increasingly associated with chronic lung infections, and describes two unique polysaccharide lyases that could be utilized as platforms for the design of highly active enzymes who substrate specificity has been fine-tuned for the problem at hand. (lehigh.edu)
  • The large substrate range of these CAZymes is compatible with the diversity of the dietary plant cell wall polysaccharides that are presented to members of the microbiota. (nature.com)
  • protein
  • however, whether this protein is a lyase has yet to be determined. (cazypedia.org)
  • Opportunistic pathogens such as Pseudomonas aeruginosa and Stenotrophomonas maltophilia colonize the lungs of cystic fibrosis patients and secrete an extracellular matrix of protein, DNA, and polysaccharides which acts as a diffusion barrier against dehydration, phagocytosis, and antibiotic treatment. (lehigh.edu)
  • Proteins
  • Proteins containing this C-terminal domain consist of a group of secreted bacterial lyase enzymes capable of acting on a variety of substrates. (ebi.ac.uk)
  • breakdown
  • 3 ] for the breakdown of C-6 carboxylated polysaccharides ( Figure 1 ), with the contrasting feature that PL-catalysed cleavage occurs without intervention of a water molecule. (biochemj.org)
  • structure
  • Crystal structure of Proteus vulgaris chondroitin sulfate ABC lyase I at 1.9A resolution. (ebi.ac.uk)
  • Due to the complex structure of plant cell walls and the variety of monosaccharide and polysaccharide cell wall components, plants encode a large variety of enzymes to synthesize, modify and degrade cell walls. (springer.com)