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Fatty Acid Synthesis InhibitorsNucleic Acid Synthesis InhibitorsCeruleninNucleic AcidsEnterobacter aerogenesRNAUridineProtein Synthesis InhibitorsMetabolismDNAThymidinePhosphorus IsotopesTritiumRNA, BacterialCarbon IsotopesPeptide Nucleic AcidsUracilHypoxanthinesCycloheximideAdenineDNA, BacterialProtein BiosynthesisPurinesPolymerase Chain ReactionKineticsEscherichia coliDNA ReplicationDNA-Directed DNA PolymeraseAnisomycinResearchBase SequenceMolecular Sequence DataRNA Polymerase IITime FactorsTranscription, GeneticRNA, MessengerFatty AcidsEmetineNucleic Acid ConformationDNA-Directed RNA PolymerasesDactinomycinPuromycinNucleic Acid HybridizationDNA Polymerase IMutationNucleic Acid ProbesCell LineAmino Acid SequenceDNA Polymerase IINucleic Acid Amplification TechniquesCells, CulturedLiverNucleic Acid DenaturationTemplates, GeneticBile Acids and SaltsDNA, ViralRNA, ViralDNA Polymerase IIIFatty Acid Synthases
InhibitionRNase Inhibitor2023ProteinsProteinFoscarnetAphidicolinAmplificationChain reactionNucleotideMRNA synthesisNovo synthesisReplicationInhibitAmino acid residuesReverse transcriptasePurificationEnzymeCDNA synthesisBacterialEnzymesBiosynthesisAssayMetabolismIsolationCatalyticTotal synthesis2019GuanineViralResearchMimicsMechanismRecombinantAbemaciclibStrandPathwayClinicalEscherichiaConcentrationsAmplifyEnzymaticGenomeQuantitativeIntracellularActivityNucleotidesTranscriptionCellRoom temperature
Inhibition7
  • Inhibition of eukaryotic DNA polymerase-alpha by polydeoxynucleotides. (neb.com)
  • Benzofuranes (WSSA Group 16), chlorocarbonic acids (Group 26), phosphorodithioates (Group 8), and thiocarbamates (Group 8) are examples of herbicides that are known inhibitors of several plant processes including: 1) biosynthesis of fatty acids and lipids which may account for reported reductions in cuticular wax deposition, 2) biosynthesis of proteins, isoprenoids (including gibberellins), and flavonoids (including anthocyanins), and 3) gibberellin synthesis inhibition which may result from the inhibition of kaurene synthesis. (weedscience.org)
  • Plant death results from events occurring in response to ALS inhibition and low branched-chain amino acid production, but the actual sequence of phytotoxic processes is unclear. (weedscience.com)
  • EPSP inhibition leads to depletion of the aromatic amino acids tryptophan, tyrosine, and phenylalanine, all needed for protein synthesis or for biosynthetic pathways leading to growth. (weedscience.com)
  • Lee 1980) suggests that factors other than protein synthesis inhibition may be involved. (weedscience.com)
  • Inhibition of fatty acid synthesis presumably blocks the production of phospholipids used in building new membranes required for cell growth. (weedscience.com)
  • 3] This antagonism might be related to topoisomerase I inhibition of DNA synthesis, which is required for the cytotoxic effect of topoisomerase II-induced cleavable complexes. (cancernetwork.com)
RNase Inhibitor1
  • RNase inhibitor is a human placenta inhibitor, which works to inhibit RNase A, B and C activity by forming a tight, non-covalent 1:1 complex. (levprot.com)
20231
  • 2023) Nucleic Acid Research, https://doi.org/10.1093/nar/gkad512. (umu.se)
Proteins2
  • The analysis involved 54 amino acid sequences, including the GluRS proteins from Methanocaldococcus jannaschii and Archaeoglobus fulgidus as an outgroup. (gp120-inhibitors.com)
  • some compounds in this group may also inhibit carotenoid biosynthesis (fluometuron) or synthesis of anthocyanin, RNA, and proteins (propanil), as well as effects on the plasmalemma (propanil) (Devine et al. (weedscience.org)
Protein9
  • While the mechanism of action of betaenone B has yet to be elucidated, betaenone C has been shown to inhibit RNA and protein synthesis. (wikipedia.org)
  • 13 , 14 However, the amino acid perfectly maintains the stability of the mutual structural conformation of the virus S-protein and the ACE2 receptor in a holistic manner. (medsci.org)
  • Real‑time quantitative polymerase chain reaction (PCR) and western blot analyses were used to detect SPRY2 expression and miR‑21 protein expression levels. (spandidos-publications.com)
  • The SPRY2 protein contains 315 human amino acid residues (35 kDa), with the C-terminal residues 178-282 being rich in cysteine. (spandidos-publications.com)
  • The complementary structure immediately suggested to Watson and Crick how DNA could (and does) replicate, and further explains how information from DNA is transferred to RNA for protein synthesis. (rainis.pics)
  • Read in groups of three, the base sequence directly gives the amino acid sequence in the encoded protein. (rainis.pics)
  • Synthesis of protein & urea, Ornithine cycle, fate of carbon skeleton. (aadharinstitute.com)
  • The architecture of virions is composed of nucleic acid and nucleocapsid protein to form the helical nucleocapsid. (newvita.com)
  • RESULTS: We expressed the gene encoding Enterococcus faecium phage IME199 DNA polymerase (IME199 DNAP) in Escherichia coli BL21(DE3), and characterized protein function. (bvsalud.org)
Foscarnet1
  • First-line and second-line antiviral drugs used to treat HSV infections, acyclovir and related compounds, as well as foscarnet and cidofovir, selectively inhibit herpesvirus DNA polymerase (DNA-pol). (bvsalud.org)
Aphidicolin2
  • The mode of inhibitory action by aphidicolin on eukaryotic DNA polymerase alpha. (neb.com)
  • Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha. (neb.com)
Amplification7
  • Conventional PCR is a qualitative technique for detection and amplification of nucleic acids. (levprot.com)
  • Isothermal nucleic acid amplification is a unique method to amplify limited amounts of DNA at constant temperature, eliminating the thermal cycling process and thus, the need of a thermocycler equipment. (levprot.com)
  • At Levprot we offer a Bst DNA polymerase which is uniquely useful for a variety of isothermal amplification reactions. (levprot.com)
  • This means that nucleic acids from the specimen have to be purified prior to amplification. (medixbiochemica.com)
  • BACKGROUND: Identification and characterization of novel, faithful and processive DNA polymerases is a driving force in the development of DNA amplification methods. (bvsalud.org)
  • More importantly, the IME199 DNAP has strand displacement and processive synthesis capabilities, and can perform rolling circle amplification and multiple displacement amplification with very low error rates (approximately 3.67 × 10-6). (bvsalud.org)
  • Based on the characterized properties, IME199 DNAP is expected to be developed as a high-fidelity polymerase for DNA amplification at room temperature. (bvsalud.org)
Chain reaction4
Nucleotide2
  • Thus, both nucleoside and nucleotide RT inhibitors (NRTIs) and non-nucleoside RT inhibitors (NNRTI) are extensively used as antiretroviral agents against HBV and HIV infection [ 1 , 2 ]. (biomedcentral.com)
  • Others join two substrates together, such as DNA polymerase linking a nucleotide to DNA . (wikipedia.org)
MRNA synthesis2
  • mRNA synthesis has gained importance since mRNA vaccines and therapeutics became relevant in biopharma. (levprot.com)
  • Synthesis of mRNA requires several steps from DNA template to pure mRNA, including post-translational modifications and additional components needed for mRNA synthesis. (levprot.com)
Novo synthesis1
  • While a complete de novo synthesis of betaenone B has yet to be reported, Daniel Pratt and Paul Hopkins in 1988 proposed a method for synthesizing a precursor of betaenone B from methoxybenzoquinone and 1,3-butadiene via a Diels-Alder reaction and Claisen chemistry[clarification needed]. (wikipedia.org)
Replication2
  • Table 35-2 describes the mode of action of antiviral drugs that block early events in viral replication, and Table 35-3 describes the mode of action of antiviral drugs that block viral nucleic acid synthesis. (mhmedical.com)
  • Deuremidevir, an oral nucleoside analog, is a broad-spectrum virus replication inhibitor targeting the highly conserved RNA-dependent RNA polymerase. (bvsalud.org)
Inhibit2
  • Imidazolinones, pyrimidinylthiobenzoates, sulfonylaminocarbonyltriazolinones, sulfonylureas, and triazolopyrimidines are herbicides that inhibit acetolactate synthase (ALS), also called acetohydroxyacid synthase (AHAS), a key enzyme in the biosynthesis of the branched-chain amino acids isoleucine, leucine, and valine (LaRossa and Schloss 1984). (weedscience.com)
  • Glycines (glyphosate) are herbicides that inhibit 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (Amrhein 1980) which produces EPSP from shikimate-3-phosphate and phosphoenolpyruvate in the shikimic acid pathway. (weedscience.com)
Amino acid residues3
  • and even the role of particular amino acid residues in the mechanism. (wikipedia.org)
  • The amino acid residues Asp30, Glu32, Asp112 and Asp251 are the 3'-5' exonuclease active sites of IME199 DNAP, while residues Asp596 and Tyr639 are essential for DNA synthesis by IME199 DNAP. (bvsalud.org)
  • Partial Order Optimum Likelihood (POOL), a machine learning method that uses input features from Theoretical Microscopic Titration Curve Shapes (THEMATICS), was used to identify amino acid residues most likely involved in catalytic activity. (bvsalud.org)
Reverse transcriptase1
  • This inhibitor does not affect the activity of Taq DNA polymerase, AMV or M-MLV reverse transcriptase or phage RNA polymerases. (levprot.com)
Purification2
  • Targets can be reliably amplified from a variety of sources without a purification step, because the polymerase exhibits a strong tolerance to common PCR inhibitors. (medixbiochemica.com)
  • DIA-UP has rich experience in RNA synthesis and complete RNA synthesis and purification technology, and can directly provide customers with high-quality RNA synthesis services or products. (diagreat.com)
Enzyme6
  • DNA polymerase-beta from the nuclear fraction of sea urchin embryos: characterization of the purified enzyme. (neb.com)
  • Each fragment was amplified from S. flexneri genomic DNA using the indicated primers with selleck the High Fidelity PCR Enzyme Mix polymerase and cloned into pQF50. (gp120-inhibitors.com)
  • The mutation in the α helix 28 of Taq Hotstart DNA Polymerase confers inactivity of the enzyme at room temperature. (medixbiochemica.com)
  • Enzyme inactivation at lower temperatures is achieved by a tight bond of the aptamer to the polymerase. (medixbiochemica.com)
  • These two candidates were incorporated by solid-phase synthesis into DNA carrying the sequence of a promoter for an RNA polymerase enzyme. (chemistryviews.org)
  • Studying an enzyme's kinetics in this way can reveal the catalytic mechanism of this enzyme, its role in metabolism , how its activity is controlled, and how a drug or a modifier ( inhibitor or activator ) might affect the rate. (wikipedia.org)
CDNA synthesis1
Bacterial3
  • Bacterial growth was moni tored by optical Inhibitors,Modulators,Libraries density at 600 nm. (gp120-inhibitors.com)
  • Hassan HM, Degen D , Jang KH, Ebright RH , Fenical W. Correction to: Salinamide F, new depsipeptide antibiotic and inhibitor of bacterial RNA polymerase from a marine-derived Streptomyces sp. (academictree.org)
  • Design, Synthesis, and Characterization of TNP-2198, a Dual-Targeted Rifamycin-Nitroimidazole Conjugate with Potent Activity against Microaerophilic and Anaerobic Bacterial Pathogens. (academictree.org)
Enzymes2
  • NGS has several enzymatic workflow steps, in which polymerases, restriction enzymes, ligases and kinases-phosphorilases have an important role. (levprot.com)
  • The main difference between ribozymes and enzymes is that RNA catalysts are composed of nucleotides, whereas enzymes are composed of amino acids. (wikipedia.org)
Biosynthesis1
Assay2
  • Lyophilized PlexTaq performs as effectively as the liquid form whilst offering the intrinsic advantages of an engineered, room-temperature-stable Taq DNA polymerase as well as reduced time for assay preparation (just add primers/probes). (medixbiochemica.com)
  • A real-time reverse transcription-polymerase chain (e.g., gel electrophoresis), were time-consuming, and were reaction (RT-PCR) assay was developed to rapidly detect prone to false-positive results from amplicon contamina- the severe acute respiratory syndrome-associated coron- tion. (cdc.gov)
Metabolism2
  • Metabolism of nucleic acids. (aadharinstitute.com)
  • Phosphatidic acid (PA) is an important intermediate in membrane lipid metabolism that acts as a key component of signaling networks, regulating the spatio-temporal dynamics of the endomembrane system and the cytoskeleton. (frontiersin.org)
Isolation2
  • RNA isolation and synthesis of cDNA Total mRNA was obtained during the growth Inhibitors,Modulators,Libraries of S. flexneri 2457T using the RNeasy mini kit following the supplier instructions. (gp120-inhibitors.com)
  • A total of 340 clinical specimens collected from 246 were quickly developed: tissue culture isolation, antibody persons with confirmed or suspected SARS-CoV infec- detection, and reverse transcription-polymerase chain tion (13) were used in this study. (cdc.gov)
Catalytic1
  • The common A467T mutation in the human mitochondrial DNA polymerase (POLG) compromises catalytic efficiency and interaction with the accessory subunit. (musc.edu)
Total synthesis3
  • While it was also shown that betaenone B could be converted to betaenone A by oxidation by PCC followed by exposure to base, it wasn't until 1988 that a semi-complete total synthesis was reported. (wikipedia.org)
  • Bioactivity of this synthetic product was not tested and no further work on total synthesis of betaenones has been published since. (wikipedia.org)
  • Elbatrawi YM, Gerrein T, Anwar A, Makwana KM , Degen D , Ebright RH , Del Valle JR. Total Synthesis of Pargamicin A. Organic Letters . (academictree.org)
20191
Guanine1
Viral1
  • The results revealed that Azvudine could reduce nucleic acid-negative conversion time, viral load, and time to improvement in clinical conditions in patients with moderate COVID-19. (bvsalud.org)
Research2
Mimics1
  • DIA-UP's advanced synthesis technology can provide customers with miRNA inhibitors, miRNA mimics, miRNA negative controls, Agomir and Antagomir. (diagreat.com)
Mechanism3
  • Tapryal N, Mukhopadhyay C, Mishra MK, Das D, Biswas S, and Mukhopadhyay C. K. Glutathione synthesis inhibitor butathione sulfoximine regulates ceruloplasmin by dual but opposite mechanism: Implication in hepatic iron overload. (jnu.ac.in)
  • Benzoic acids, phenoxycarboxylic acids, pyridine carboxylic acids, and quinoline carboxylic acids (O(4) and L(27)) are herbicides that act similar to that of endogenous auxin (IAA) although the true mechanism is not well understood. (weedscience.com)
  • 3] This is believed to constitute an important mechanism of resistance to topoisomerase I inhibitors in malignant cells. (cancernetwork.com)
Recombinant1
  • Recombinant HIPKs and DYRK1A are auto-activated and phosphorylate the negative elongation factor SPT5, the transcription factor c-Myc, and the C-terminal domain of RNA polymerase II, suggesting a direct function in transcriptional regulation. (nature.com)
Abemaciclib2
  • Based on a database search, we identified abemaciclib, an FDA-approved Cdk4/Cdk6 inhibitor used for the treatment of metastatic breast cancer, as potent inhibitor of HIPK2, HIPK3, and DYRK1A. (nature.com)
  • A third inhibitor, abemaciclib was FDA approved in September 2017. (medscape.com)
Strand1
  • All organisms-bacteria, archaea, and eukaryotes-have a transcription initiation factor that contains a structural module that binds within the RNA polymerase (RNAP) active-center cleft and interacts with template-strand single-stranded DNA (ssDNA) in the immediate vicinity of the RNAP active center. (osti.gov)
Pathway1
  • The backbone carbon units of betaenone B are known to be synthesized via the polyketide synthesis (PKS) pathway. (wikipedia.org)
Clinical2
  • Being susceptible to potential inhibitors from clinical specimens. (medixbiochemica.com)
  • Nucleic acids were recovered from clinical specimens cies in this study were selected for further study (Table 1). (cdc.gov)
Escherichia1
Concentrations1
  • The task was to test whether lead, mercury, copper, zinc and benzene in environmentally relevant concentrations influence porphyrin synthesis of cells. (who.int)
Amplify1
  • In addition, IME199 DNAP has dNTP-dependent 5'-3' polymerase activity and can amplify DNA at 15-35 °C and a pH range of 5.5-9.5. (bvsalud.org)
Enzymatic1
  • The DNA turned out to be photoresponsive and allowed to modulate the rate of enzymatic RNA synthesis by light. (chemistryviews.org)
Genome1
  • Even if poxvirus genome synthesis projects were to be undertaken, the creation of a synthetic variola virus is prohibited by existing regulations. (who.int)
Quantitative1
  • However, real time PCR, also known as quantitative PCR (qPCR), allows sensitive and specific quantification of nucleic acids. (levprot.com)
Intracellular2
  • Changes in intracellular location of DNA polymerase-alpha during oocyte maturation of the toad, Bufo bufo japonicus. (neb.com)
  • Cheng A, Wan D, Ghatak A, Wang C , Feng D, Fondell JD, Ebright RH , Fan H. Identification and Structural Modeling of the RNA Polymerase Omega Subunits in Chlamydiae and Other Obligate Intracellular Bacteria. (academictree.org)
Activity3
  • TDF is a non-selective RT inhibitor with demonstrated in vitro activity against both wild-type and mutant strains of HIV [ 4 - 6 ] and is also effective for treating chronic hepatitis B (CHB) [ 7 - 10 ]. (biomedcentral.com)
  • In preclinical models of MM, synergy was observed in anti-tumor activity when poly (ADPribose) polymerase (PARP) inhibitors (olaparib, talazoparib) were used in combination with melphalan. (haematologica.org)
  • Conversely, suppressing PA degradation by inhibiting LPP activity using any of three different inhibitors significantly stimulated pollen tube growth, and similar effect was achieved by suppressing the expression of tobacco pollen LPP4 using antisense knock-down. (frontiersin.org)
Nucleotides1
  • Why, then, do cosubstrates so often possess nucleotides as their binding tags, rather than for example amino acids or peptides? (heresy.is)
Transcription1
  • In transcription antitermination by Qλ, NusA induces refolding of Qλ to form a nozzle that extends the RNA polymerase RNA-exit channel. (academictree.org)
Cell2
  • Nucleic acids, DNA and RNA, can be considered as the information molecules of the cell. (rainis.pics)
  • The model used was the permanent epithelial larynx carcinoma cell line HEp-2, which was stimulated to synthesize porphyrins by adding δ-amino-levulinic acid (δ-ALA) in excess. (who.int)
Room temperature1
  • 1-4 Medix Biochemica's unique engineered Taq DNA polymerase variant, which is used in PlexTaq ® products, is very stable at room temperature, offers a fast start feature and exhibits high specificity and sensitivity in a PCR setup. (medixbiochemica.com)