Polymerase chain reaction. Medical search. FAQ

Flow immunophenotyping, DNA content analysis, and polymerase chain reaction (PCR) amplification for t(11;14) and t(14;18) were performed on 11 cases of typical mantle cell lymphoma (MCL), 5 cases of apparent MCL with proliferation centers (MCL-PC), and 5 cases of small lymphocytic lymphoma (SLL). (ashpublications.org)
We have successfully overcome this problem with a novel combination of two widely used approaches: target-specific multiplex amplification with 15 cycles of polymerase chain reaction (PCR), followed by single-molecule detection of amplicons with atomic force microscopy (AFM). (elsevier.com)

Then, to perform PCR, the DNA template that contains the target is added to a tube that contains primers, free nucleotides, and an enzyme called DNA polymerase, and the mixture is placed in a PCR machine. (latestgkgs.com)
The DNA polymerase begins to synthesize new strands of DNA starting from the primers. (latestgkgs.com)

Low-level malaria infections detected by a sensitive polymerase chain reaction assay and use of this technique in the evaluation of malaria vaccines in an endemic area. (ox.ac.uk)

Polymerase Chain Reaction (PCR) is one of the most important molecular diagnostic tools which allow the detection of nucleic acid targets. (scialert.net)
Nucleic acid hybridisation and polymerase chain reaction in the the use of nucleic acid hybridisation and polymerase methods and does not, Application of polymerase chain reaction for detection of goats' milk adulteration by milk of cow - Volume 68 Issue 2 - JACEK BANIA, MACIEJ UGORSKI, ANTONI POLANOWSKI. (hochregal.net)
US7393644B2 Method for real-time detection of polymerase. (hochregal.net)
Detection of human papillomavirus type 16 DNA sequences in archival cervical tissues by the polymerase chain reaction. (ox.ac.uk)
We have evaluated the polymerase chain reaction for the detection of viral DNA sequences in paraffin-embedded archival tissues. (ox.ac.uk)
In three Southern blotting-positive cases, the DNA of the paraffin-embedded sections was too scant or too degraded to allow the detection of HPV DNA by the polymerase chain reaction. (ox.ac.uk)
In 21 Southern blotting-negative cases, HPV type 16 DNA could be demonstrated in the archival sections by the polymerase chain reaction technique--a sensitivity improvement of more than 80% over the standard method of HPV detection in tissues. (ox.ac.uk)

Global Polymerase Chain Reaction Technologies Industry valued approximately USD 7 million in 2016 is anticipated to grow with a healthy growth rate of more than 7% over the forecast period 2019-2025. (globalreportsstore.com)

Using barcodes and barcode databases, species specific Quantitative Polymerase Chain Reaction (qPCR) assays can be designed to detect the presence of target species where traditional sampling is inefficient or impossible. (confex.com)

The polymerase chain reaction Application-specific PCR F.A. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. (hochregal.net)
The methods and systems may The polymerase chain reaction Application-specific PCR F.A. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. (hochregal.net)

Digital Polymerase Chain Reaction (dPCR) is an advancement of traditional polymerase chain reaction (PCR). (bigmarketresearch.com)
The major factors driving the global digital polymerase chain reaction market are increasing demand for innovative diagnostic techniques, increasing disease awareness, need for early diagnosis of viral, infectious and genetic disease, and increasing number of diagnostic centers across the globe. (bigmarketresearch.com)

Polymerase chain reaction( PCR) is a laboratory technique used to make multiple copies of a segment of DNA. (latestgkgs.com)

A novel temperature control method for shortening thermal cycling time to achieve rapid polymerase chain reaction (PCR) in a disposable polymer microfluidic device. (dtu.dk)
We present a new temperature control method capable of effectively shortening the thermal cycling time of polymerase chain reaction (PCR) in a disposable polymer microfluidic device with external heater and temperature sensor. (dtu.dk)

1/03/2009В В· Principles and applications of polymerase chain reaction in a broad range of medical diagnostic fields, methods, Polymerase Chain Reaction, You have free access to this content The polymerase chain reaction and its application in occupational and environmental medicine [Biomonitoring Methods, (hochregal.net)
Un examen rétrospectif des dossiers pré- et post-intervention a servi à décrire le séjour à l'hôpital des patients ayant reçu un diagnostic de BAC-SA avant et après la mise en place du système de diagnostic de la PCR. (cjhp-online.ca)
Au total, 98 et 99 patients ont satisfait au critère d'inclusion respectivement avant et après la mise en place du système de diagnostic de la PCR. (cjhp-online.ca)

Polymerase Chain Reaction invented a method of amplifying any DNA region through repeated cycles of implications for research applications and in forensics. (hochregal.net)

17/04/2014В В· Polymerase Chain Reaction (PCR) Steps, Requirements and Applications biologyexams4u. (hochregal.net)

Polymerase Chain Reaction size and sequence by enzymatic method and cycling condition. (hochregal.net)
This powerful method has numerous applications in Theory and applications of the polymerase chain Theory and applications of the polymerase chain reaction. (hochregal.net)
Learn about PCR (polymerase chain reaction) a method of analyzing a short sequence of DNA or RNA. (hochregal.net)
Polymerase Chain Reaction Validation, Optimization, Limitations and Applications The method is useful for coding the genetic sequence of various disease, Polymerase Chain Reaction (PCR) In Medical Application: An Analytical Report 2014-2020 - The global PCR market is projected to reach around US$27.4 billion by 2015. (hochregal.net)

PCR Methods Appl 1, 17 Genetic applications of an inverse polymerase chain reaction. (hochregal.net)
Methods Enzymol https://simple.wikipedia.org/wiki/Polymerase_chain_reaction Polymerase Chain Reaction. (hochregal.net)
Methods and systems for polymerase chain reactions (PCR) that are capable of detecting amplified DNA during or after the PCR process. (hochregal.net)

In test experiments comparing the relative expression of ten transcripts in two different human total RNA samples, we find good agreement between our single reaction, multiplexed PCR/AFM data, and data from 20 individual singleplex quantitative PCR reactions. (elsevier.com)

In MCL-PC, the mean intensity of IgM, Ig light chain, and CD20 expression was intermediate to the intensities observed in MCL and SLL. (ashpublications.org)

In the polymerase chain reaction analysis of the formalin-fixed, paraffin-embedded mirror biopsy specimens, 46 (73%) of the tissues were found to be positive for HPV type 16. (ox.ac.uk)
Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. (moluna.de)

PDF) Polymerase chain reaction Theory practice and. (hochregal.net)

The study of Polymerase Chain Reaction (PCR) Products market is a compilation of the market of Polymerase Chain Reaction (PCR) Products broken down into its entirety on the basis of types, application, trends and opportunities, mergers and acquisitions, drivers and restraints, and a global outreach. (dsportstimes.com)
Along with a generalized market study, the report also consists of the risks that are often neglected when it comes to the Polymerase Chain Reaction (PCR) Products industry in a comprehensive manner. (dsportstimes.com)
For a global outreach, the Polymerase Chain Reaction (PCR) Products study also classifies the market into a global distribution where key market demographics are established based on the majority of the market share. (dsportstimes.com)

During a typical PCR, template DNA (containing the region of interest) is mixed with deoxynucleotides (dNTPs), a DNA polymerase and primers. (neb.com)
Primers are short segments of DNA that are complementary with the template DNA upstream of the region of interest and serve as recruitment sites for the polymerase. (neb.com)
The single-stranded sequences generated by denaturing are used as templates for the primers and the DNA polymerase. (psu.edu)
Primers bind to the strand to give the polymerase something to extend. (physicsforums.com)
PCR employs two main reagents - primers (which are short single strand DNA fragments known as oligonucleotides that are a complementary sequence to the target DNA region) and a DNA polymerase . (wikipedia.org)
A PCR solution is made similarly to a TaqMan assay, which consists of template DNA (or RNA), fluorescence-quencher probes, primers, and a PCR master mix, which contains DNA polymerase, dNTPs, MgCl2, and reaction buffers at optimal concentrations. (wikipedia.org)
A labelled probe is included in the PCR reagent mix in addition to the primers used in a traditional PCR reaction. (abpischools.org.uk)
Both reactions are run for the same length of time in identical conditions (preferably using the same primers, or at least primers of similar annealing temperatures). (bionity.com)
Using agarose gel electrophoresis separate the products of the reaction from their original DNA and spare primers. (bionity.com)
PCR works the same way, but instead of a banquet hall full of wedding guests, it takes place in a test tube full of DNA templates and primers, polymerase, and nucleotides. (scienceline.org)
Rychlik W (1993) Selection of primers for polymerase chain reaction. (els.net)
Sample DNA , nucleotides, DNA primers & thermostable DNA polymerase placed in PCR machine. (slideserve.com)
PCR requires template DNA, Taq polymerase, di-deoxynucleic acids with each of the four DNA bases, Mg2+, primers and a buffer. (slideserve.com)
We developed a single multiplex PCR reaction to detect ETEC, EPEC, EIEC, and STEC, using specific previously described ( 4 - 6 ) or new primers (GIBCO-BRL, Gaithersburg, MD) for diverse virulence traits ( Table 1 ). (cdc.gov)
Taq DNA polymerase is known to be contaminated with low levels of bacterial DNA not originating from either Thermus aquaticus or Escherichia coli and is easily amplified using universal bacterial primers based on ribosomal gene sequences. (bmj.com)
The specific Taq used in the study (AmpliTaq DNA polymerase) is well known for being unsuitable for bacterial PCR using pan-bacterial primers such that the company itself (Perkin-Elmer) has more recently introduced a "low DNA" Taq (Amplitaq LD) in order to reduce the size of the problem. (bmj.com)
During PCR, the template DNA is denatured to single-strands at a high temperature, then allowed to anneal with an excess of primers at a lower temperature, followed by synthesis of DNA or RNA from the primers at a temperature optimum for the specific polymerase enzyme. (ubc.ca)
A sample of the supernatant containing chromosomal DNA is mixed with Taq DNA polymerase, oligonucleotide primers, the four deoxyribonucleotides, and the cofactor magnesium chloride. (ubc.ca)
uses a thermostable polymerase (S-Tbr) that can extend from short primers ("smalligos") as short as 9 or 10 nucleotides. (primidi.com)
Oligonucleotide consensus primers were designed to anneal to any of the four dengue virus types and amplify a 511-bp product in a reverse transcriptase-polymerase chain reaction (PCR). (asm.org)
The current study was designed to develop and validate a rapid and accurate diagnostic assay to detect two important genera of dermatophytes in dogs, Microsporum and Trichophyton differentially by a uniplex PCR reaction.18S ribosomal RNA gene of both the genera was used to design common forward and reverse primers. (thefreelibrary.com)
The PCR process is completed in vitro and utilizes a DNA preparation containing the desired segment to be target sequence , two nucleotide primers about 20 bases long specific, the four deoxynucleoside triphosphates and a heat stable DNA polymerase. (assignmenthelp.net)
Three essential steps to PCR (Figure 1) include (a) melting of the target (b) annealing of two oligonucleotide primers to the denatured DNA strands, and (c) primer extension by a thermostable DNA polymerase (123). (unl.edu)
External primers were used to amplify a fragment of the expected size (441 bp) in all the samples evaluated using reverse transcriptase polymerase chain reaction (RT-PCR), but with very low intensity. (eurekamag.com)
In the next step, The reaction mixture is cooled (about 50-65 o C) for 20-40 seconds, to allow primers to select and bind (hybridize) to their complementary positions on the ssDNA template molecules. (mybiosource.com)

DNA polymerases, whether from humans, bacteria, or viruses, cannot copy a chain of DNA without a short sequence of nucleotides to "prime" the process, or get it started. (accessexcellence.org)
A PCR vial contains all the necessary components for DNA duplication: a piece of DNA, large quantities of the four nucleotides, large quantities of the primer sequence, and DNA polymerase. (accessexcellence.org)
The Taq polymerase begins adding nucleotides to the primer and eventually makes a complementary copy of the template. (accessexcellence.org)
The two DNA strands then become templates for DNA polymerase to enzymatically assemble a new DNA strand from free nucleotides , the building blocks of DNA. (wikipedia.org)
Chemical compound made up of long chains of nucleotides joined together e.g. (abpischools.org.uk)
Photochemical control of the polymerase chain reaction has been achieved through the incorporation of light-triggered nucleotides into DNA. (rsc.org)
Direct solid-phase sequence analysis of the human p53 gene by use of multiplex polymerase chain reaction and alpha-thiotriphosphate nucleotides. (diva-portal.org)

96 QPCR reactions can be setup in less than 10 minutes. (sigmaaldrich.com)
Quantitative polymerase chain reaction (qPCR) is a modification of the polymerase chain reaction used to rapidly measure the quantity of DNA , complementary DNA or ribonucleic acid present in a sample. (bionity.com)
In real-time RT-PCR, also known as quantitative RT-PCR (RT-qPCR), data is collected while the reaction is occurring. (golden.com)
Real-time quantitative polymerase chain reaction analysis combined with reverse transcription (RT-qPCR) has been widely used to measure the level of RNA virus infections. (jove.com)
Merck's One Step RT-PCR kits , NovaTaq™ Polymerase mastermixes and NovaQUANT™ qPCR assay kits make real-time PCR accessible, reproducible and standardized. (merckmillipore.com)

We wished to develop an accurate quantitative protocol based on a droplet digital polymerase chain reaction (ddPCR) involving eight individual TaqMan™ reactions to detect simultaneously, without selective enrichment, Listeria spp. (frontiersin.org)

The most sensitive method uses ligation-mediated polymerase chain reaction (LM-PCR) to amplify all fragments of the genomic sequence ladder (3,4). (nih.gov)

It uses thermal cycler including repeated heating and cooling mechanisms for melting of DNA and replication using thermo stable DNA polymerase, primer with specific complementary sequence of the target region and dNTPs. (mybiosource.com)
The PCR reaction begins with a high temperature of 94-98 °C for an extended period of 1-9 minutes to activate the heat stable DNA polymerase. (mybiosource.com)

In the following we will briefly summarize our recent experience with the application of polymerase chain reaction (PCR) strategies to patients with chronic myelocytic leukemia (CML) and acute lymphoblastic leukemia (ALL) in complete remission according to clinical and laboratory parameters. (springer.com)

3) Finally, the DNA-Polymerase has to fill in the missing strands. (slideshare.net)
As the two strands separate, DNA polymerase makes a copy using each strand as a template. (accessexcellence.org)
The three steps in the polymerase chain reaction - the separation of the strands, annealing the primer to the template, and the synthesis of new strands - take less than two minutes. (accessexcellence.org)
It helps to use a DNA polymerase that is not denatured by the high temperature needed to separate the DNA strands. (biology-pages.info)

2. The Polymerase Chain Reaction The PCR uses the mechanism of DNA replication. (psu.edu)
As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the original DNA template is exponentially amplified. (wikipedia.org)
An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. (google.com)
PCR involves replication of the DNA template by a thermostable DNA polymerase. (emdmillipore.com)
this reaction is the same as that occurs in vivo during replication of the leading strand of a DNA duplex. (assignmenthelp.net)
The key to the replication reaction is that it is driven by a heat-stable polymerase molecule that reads a template DNA in the 3'-5' direction and synthesises a new complementary template in the 5'-3' direction, using free dideoxy nucleoside triphosphates (dNTP's = nucleotide bases) as building blocks. (mybiosource.com)
Taq polymerase shows highest activity at the temperature range of 75-78 o C. The ssDNA/primer solution is thus heated to 72 o C. In the presence of the heat stable polymerase, PCR buffer, dNTP's and magnesium (Mg2+) molecules, the replication procedure begins. (mybiosource.com)

i.e. this will be a heat stable polymerase. (ubc.ca)

Analysis of major BCR-ABL1 mRNA by digital polymerase chain reaction is useful for prediction of international scale. (bioportfolio.com)

This study aimed to establish an accurate and sensitive polymerase chain reaction [‎PCR]‎ technique for the diagnosis of active human brucellosis in Egypt. (who.int)
Comparative Evaluation of Immunochromatographic and Reverse Transcriptase Polymerase Chain Reaction based tests for Diagnosis of Canine Distemper. (thefreelibrary.com)
2014). This study is an attempt to compare polymerase chain reaction based molecular tests with commercially available IC based test for diagnosis of Canine distemper by testing rectal swabs and serum samples respectively from dogs suffering from gastroenteritis and suspected for Canine distemper. (thefreelibrary.com)
Development and evaluation of a real-time polymerase chain reaction for fast diagnosis of sporotrichosis caused by Sporothrix globosa. (bioportfolio.com)
polymerase chain reaction for fast diagnosis of sporotrichosis caused by Sporothrix globosa. (bioportfolio.com)
The recent development of novel Polymerase Chain Reaction (PCR) technologies that confer theoretical advantages over quantitative PCR has considerable potential in the diagnosis of low load infections. (bioportfolio.com)
Is Polymerase Chain Reaction in Neonatal Dried Blood Spots Reliable for the Diagnosis of Congenital Cytomegalovirus Infection? (bioportfolio.com)

With traditional PCR, the amount of product can only be worked out at the end of the reaction (the plateau phase), typically by agarose gel electrophoresis. (abpischools.org.uk)

If the polymerase used was heat-susceptible, it would denature under the high temperatures of the denaturation step. (wikipedia.org)
It may be performed manually by heating the reaction components to the denaturation temperature (e.g., 95°C) before adding the polymerase. (primidi.com)
First, we produced a cDNA copy of a portion of the viral genome in a reverse transcriptase reaction in the presence of primer D2 and then carried out a standard PCR (35 cycles of heat denaturation, annealing, and primer extension) with the addition of primer D1. (asm.org)
The reaction mixture is first heated to a temperature between 90-98°C that ensures DNA denaturation and this step is called denaturation step. (assignmenthelp.net)

This is a machine that heats and cools the reaction tubes within it to the precise temperature required for each step of the reaction. (slideshare.net)
Since the Taq polymerase works best at around 75 degrees C (the temperature of the hot springs where the bacterium was discovered), the temperature of the vial is raised. (accessexcellence.org)
Taking into account the time it takes to change the temperature of the reaction vial, 1 million copies can be ready in about three hours. (accessexcellence.org)
HOWEVER, we do have one problem: the high temperature used to open up the helical DNA molecule will basically knacker out any protein structures,including our polymerase. (ubc.ca)
A recent modification on this process, known as L inear- A fter- T he- E xponential-PCR (LATE-PCR), uses a limiting primer with a higher melting temperature (Tm) than the excess primer to maintain reaction efficiency as the limiting primer concentration decreases mid-reaction. (primidi.com)
Hot-start/cold-finish PCR is achieved with new hybrid polymerases that are inactive at ambient temperature and are instantly activated at elongation temperature. (primidi.com)
Temperature-controlled supply chains (cold chains) require an unbroken chain of refrigeration to maintain product quality and safety. (bioportfolio.com)
In general, the PCR reaction is based on 20-40 repeated cycles with temperature change. (mybiosource.com)

KOD Hot Start polymerase amplifies genomic DNA up to 12 kbp and plasmid DNA up to 21 kbp, including GC-rich regions. (emdmillipore.com)
KOD Xtreme™ Hot Start Polymerase amplifies GC-rich targets more efficiently than other polymerases. (emdmillipore.com)

Repeated thermocycling unzips each DNA molecule, allowing polymerase to grab free floating bases and rebuild the other side of the chain. (scienceline.org)

MilliporeSigma's molecular biologists work to develop the newest, best performing polymerases for customer use, optimizing conditions, buffer compositions, and cycling parameters to save you valuable time and resources. (emdmillipore.com)

PCR inhibitors usually affect PCR through interaction with DNA or interference with the DNA polymerase. (wikipedia.org)
As well as methods for the removal of inhibitors from samples before PCR, some DNA polymerases offer varying resistance to different inhibitors and increasing the concentration of the chosen DNA polymerase also confers some resistance to polymerase-targeted inhibitors. (wikipedia.org)
Polymerase inhibitors present in both foods and enrichment media were removed efficiently. (biomedsearch.com)

Polymerase chain reaction analysis of two biopsy tissues using the Cobas TaqMan revealed a positive result for Mycobacterium avium and a negative result for Mycobacterium tuberculosis . (hindawi.com)
However, polymerase chain reaction analysis of a cultured colony of acid-fast bacteria from biopsy tissue using the Cobas TaqMan and an alternative polymerase chain reaction analysis of biopsy tissue yielded discordant results. (hindawi.com)
TaqMan polymerase chain reaction was developed to quantify the number of Bifidobacterium . (ajol.info)

contribs ) (Created page with 'A procedure that enzymatically amplifies a DNA polymerase. (webref.org)
A procedure that enzymatically amplifies a DNA polymerase. (webref.org)

Polymerase chain reaction ( PCR ) is a method widely used in molecular biology to make many copies of a specific DNA segment. (wikipedia.org)
A mainstay of virtually every molecular biology lab, polymerase chain reaction (PCR) is an easy and affordable method for amplifying specific fragments of DNA by several orders of magnitude. (sigmaaldrich.com)
The invention of polymerase chain reaction has proved a revolutionary step for molecular biology. (mybiosource.com)

A common method of amplifying target sequences of DNA in vitro by polymerase chain reaction (PCR) is described in this simplified yet accurate animation. (merlot.org)
In a multiplex PCR reaction, it is possible for the different sequences to suffer from different inhibition effects to different extents, leading to disparity in their relative amplifications. (wikipedia.org)
Successful polymerase chain reaction (PCR) is crucial for amplifying DNA sequences in order to study their function, either by sequencing, mutation, transcription, or expression of gene products. (emdmillipore.com)
In Situ Polymerase Chain Reaction ( In situ PCR) is a powerful method that detects minute quantities of rare or single-copy number nucleic acid sequences in frozen or paraffin-embedded cells or tissue sections for the localization of those sequences within the cells. (biogenex.com)

Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. (semanticscholar.org)

The purpose of this study is to demonstrate the efficacy of active study vaccine in the prevention of reverse transcriptase polymerase chain reaction (RT-PCR) confirmed respiratory syncytial virus (RSV)-mediated lower respiratory tract disease (LRTD), when compared to placebo. (centerwatch.com)
BACKGROUND Real-time reverse transcription polymerase chain reaction (RT-PCR) is routinely used to detect viral infections. (bireme.br)
Reverse-transcriptase polymerase chain reaction (RT-PCR) is a widely used method that enables transcriptional profiling and sequencing analysis on bulk populations of cells. (eurekamag.com)
An analysis of the transcript level of the 10 members of the family has been performed using the technique of real-time quantitative reverse transcriptase-polymerase chain reaction. (plantphysiol.org)

Introduction The polymerase chain reaction (PCR) is a method that uses test tubes in biological laboratories for producing large amount of identical copies of a specific gene from small amount of complex molecules. (psu.edu)
CG island methylation changes near the GSTP1 gene in prostatic carcinoma cells detected using the polymerase chain reaction: a new prostate cancer biomarker. (aacrjournals.org)

Q-PCR, Assembly PCR, Inverse PCR, RT-PCR and Digital PCR the Polymerase Chain Reaction (PCR) market to witness a value of US$12 billion by 2020. (reportsnreports.com)

9. A method in accordance with claim 1 wherein said first subset of PCR reagents consists of all PCR reagents except a nucleic acid polymerase. (google.es)
Furthermore, automation has been facilitated, as reaction buffers and additional reagents can be rapidly changed without centrifugation or precipitation steps. (springer.com)

Following cell lysis Chelex will bind to and remove the proteins and metal ions that inhibit the PCR reaction, but will leave the DNA in the solution. (ubc.ca)
In the present study, a genotyping method based on polymerase chain reaction (PCR) for the repeat regions of four genes (sdrG, sdrF, aap and sesE) that encode for bacterial surface proteins was developed and applied to a sample of well-characterised neonatal blood isolates of S. epidermidis (n = 49). (diva-portal.org)

The levels of DNA contamination are easily detectable at the sensitivity (40 fg) for the second round PCR reported by this group of authors and neither in the text nor in the figures is there any mention of a first round negative control as a test sample in the nested PCR reaction. (bmj.com)
It was determined whether Borrelia burgdorferi DNA may be detectable by polymerase chain reaction (PCR) in synovial membrane (SM) when PCR results from synovial fluid (SF) had become negative after antibiotic therapy. (bmj.com)

The contamination of Taq polymerase by bacterial DNA is now well established in the published press. (bmj.com)

The most sensitive quantification methods are done by the real-time polymerase chain reaction , where the amount of DNA is measured after each cycle of PCR by use of fluorescent markers. (bionity.com)

In this report, we describe a novel droplet-based microfluidic system for performing ~50000 single-cell RT-PCR reactions in a single experiment while consuming a minimal amount of reagent. (eurekamag.com)

NovaTaq™ DNA polymerase is a premium quality recombinant form of Thermus aquaticus (Taq) DNA polymerase. (merckmillipore.com)
NovaTaq™ master mixes have all the advantages of NovaTaq™ polymerase, plus the convenient setup and lower risk of cross contamination associated by the master mix format. (merckmillipore.com)

DNA-based procedures are becoming increasingly common within the analytical laboratory where the polymerase chain reaction (PCR) has become an indispensable technique. (rsc.org)
To standardise and apply nested Polymerase Chain Reaction (n PCR) as a rapid and reliable laboratory diagnostic test to detect Toxoplasma gondii (T.gondii) in aqueous humor (AH) and peripheral blood samples of clinically suspected toxoplasma chorioretinitis patients. (arvojournals.org)

The polymerase chain reaction may not be exponential for the first several cycles, and furthermore, plateaus eventually, so care must be taken to measure the final amount of DNA while the reaction is still in the exponential growth phase. (bionity.com)
KOD polymerase yields more product in fewer cycles compared to other PCR enzymes. (emdmillipore.com)
With each repetition of this cycle, the target is doubled and soon, after about 30 cycles, the reaction will yield in excess of 1 million copies of the target DNA fragment. (mybiosource.com)

Quantitative PCR reaction setup is tedious and time consuming. (sigmaaldrich.com)
Automated methods have been developed and validated for Quantitative PCR reaction setup. (sigmaaldrich.com)

If the template contains a G, it adds a C to the new chain, and so on to the end of the DNA strand. (accessexcellence.org)
In a PCR reaction DNA polymerase is responsible for building the new strand of DNA. (physicsforums.com)
However because of the mechanism by which DNA polymerase works it cannot just build a new strand opposite the old, it can only build off of existing DNA. (physicsforums.com)

The DNA polymerase copies the DNA again. (slideserve.com)
Polymerase Chain Reaction (PCR) is a technique used by Molecular Biologists to expand a sample to create millions of copies of tiny fragments of DNA. (ccoex.com)

Mycobacterium tuberculosis isolates from previously treated patients (n = 88) from all regions of Syrian Arab Republic were characterized in terms of antibiotic sensitivity and genotyping using double-repetitive-element polymerase chain reaction (DRE-PCR) method for the proximity of the repetitive DNA elements IS6110 (a mobile genetic element) and PGRS. (who.int)

The purpose of this study is to compare the diagnostic efficacy of nested and realtime polymerase chain reaction for Mycobacterium tuberculosis using EBUS-TBNA samples in patients with iso. (bioportfolio.com)

The polymerase chain reaction, now widely used in research laboratories and doctor's offices, relies on the ability of DNA-copying enzymes to remain stable at high temperatures. (accessexcellence.org)
When any cell divides, enzymes called polymerases make a copy of all the DNA in each chromosome. (accessexcellence.org)

Recently, the application of the polymerase chain reaction (peR) technique developed by K. D. Mullis and detailed in the study by Saiki et al. (springer.com)
PCR (Polymerase chain reaction) was developed by Kary Mullis in the mid-1980s. (slideserve.com)

Since its introduction in the mid-80s, polymerase chain reaction (PCR) technology has been recognised as a rapid, sensitive and specific molecular diagnostic tool for the analysis of micro-organisms in clinical, environmental and food samples. (nih.gov)
This report presents a case of tuberculous lymphadenitis that was difficult to diagnose using polymerase chain reaction analysis. (hindawi.com)
Analysts at TMR estimate it to rise at a CAGR of 6.60% during the period from 2014 to 2020 and reach a value of US$9.6 bn by the end of the forecast period.The research report is titled Polymerase Chain Reaction (PCR) Market - Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020. (openbroadcast.de)

Ultra-rapid flow-through polymerase chain reaction microfluidics using vapor pressure. (biomedsearch.com)
The purpose of this study is to evaluate the test characteristics of a rapid intrapartum real- time polymerase chain reaction (RT-PCR) compared to the intrapartum GBS culture as the standa. (bioportfolio.com)

Anatomy16

Organisms18

Diseases20

Chemicals and Drugs66

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Phenomena and Processes84

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Anthropology, Education, Sociology and Social Phenomena1

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Assay1

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Application of polymerase chain re1

Strands4

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Heat stable polymerase1

Digital Polymera1

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Denaturation4

Temperature8

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