• With traditional PCR, the amount of product can only be worked out at the end of the reaction (the plateau phase), typically by agarose gel electrophoresis. (abpischools.org.uk)
  • In a multiplex PCR reaction, it is possible for the different sequences to suffer from different inhibition effects to different extents, leading to disparity in their relative amplifications. (wikipedia.org)
  • Successful polymerase chain reaction (PCR) is crucial for amplifying DNA sequences in order to study their function, either by sequencing, mutation, transcription, or expression of gene products. (emdmillipore.com)
  • MilliporeSigma's molecular biologists work to develop the newest, best performing polymerases for customer use, optimizing conditions, buffer compositions, and cycling parameters to save you valuable time and resources. (emdmillipore.com)
  • To order a free set of ABPI schools posters on the following topics: biotechnology, cloning, genetic engineering, unravelling the genome, polymerase chain reaction and stem cells, please fill in our order form . (abpischools.org.uk)
  • A mainstay of virtually every molecular biology lab, polymerase chain reaction (PCR) is an easy and affordable method for amplifying specific fragments of DNA by several orders of magnitude. (sigmaaldrich.com)
  • DNA-based procedures are becoming increasingly common within the analytical laboratory where the polymerase chain reaction (PCR) has become an indispensable technique. (rsc.org)
  • In the following we will briefly summarize our recent experience with the application of polymerase chain reaction (PCR) strategies to patients with chronic myelocytic leukemia (CML) and acute lymphoblastic leukemia (ALL) in complete remission according to clinical and laboratory parameters. (springer.com)
  • In the case of RT-PCR, these are labelled with a fluorescent reporter dye at the 5' end and a quencher dye at the 3' end and only produce fluorescence when DNA polymerase moves along the template and cleaves (splits) the probe. (abpischools.org.uk)
  • As the DNA polymerase moves along the template, the probe is cleaved (broken) between the reporter and quencher dye. (abpischools.org.uk)
  • However there may be considerable cross-reaction between related species, and this is the case with the BT and EHD serogroups. (scribd.com)
  • The amount of amplified product is determined by the available substrates in the reaction, which become limiting as the reaction progresses. (wikipedia.org)
  • Like all brilliant ideas, the polymerase chain reaction is incredibly simple yet extremely clever at the same time. (abpischools.org.uk)
  • RT-PCR is a version of the polymerase chain reaction which allows the DNA to be amplified and quantified in real time. (abpischools.org.uk)
  • Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. (nih.gov)
  • This overview will walk you through how the Polymerase Chain Reaction (PCR) works. (neb.com)
  • The three parts of the polymerase chain reaction are carried out in the same vial, but at different temperatures. (accessexcellence.org)
  • TO get around this, let's use a polymerase from a bug that grows in high temperatures (thermophile). (ubc.ca)