• Flow immunophenotyping, DNA content analysis, and polymerase chain reaction (PCR) amplification for t(11;14) and t(14;18) were performed on 11 cases of typical mantle cell lymphoma (MCL), 5 cases of apparent MCL with proliferation centers (MCL-PC), and 5 cases of small lymphocytic lymphoma (SLL). (ashpublications.org)
  • We have successfully overcome this problem with a novel combination of two widely used approaches: target-specific multiplex amplification with 15 cycles of polymerase chain reaction (PCR), followed by single-molecule detection of amplicons with atomic force microscopy (AFM). (elsevier.com)
  • Global Polymerase Chain Reaction Technologies Industry valued approximately USD 7 million in 2016 is anticipated to grow with a healthy growth rate of more than 7% over the forecast period 2019-2025. (globalreportsstore.com)
  • Digital Polymerase Chain Reaction (dPCR) is an advancement of traditional polymerase chain reaction (PCR). (bigmarketresearch.com)
  • The major factors driving the global digital polymerase chain reaction market are increasing demand for innovative diagnostic techniques, increasing disease awareness, need for early diagnosis of viral, infectious and genetic disease, and increasing number of diagnostic centers across the globe. (bigmarketresearch.com)
  • A novel temperature control method for shortening thermal cycling time to achieve rapid polymerase chain reaction (PCR) in a disposable polymer microfluidic device. (dtu.dk)
  • We present a new temperature control method capable of effectively shortening the thermal cycling time of polymerase chain reaction (PCR) in a disposable polymer microfluidic device with external heater and temperature sensor. (dtu.dk)
  • 1/03/2009В В· Principles and applications of polymerase chain reaction in a broad range of medical diagnostic fields, methods, Polymerase Chain Reaction, You have free access to this content The polymerase chain reaction and its application in occupational and environmental medicine [Biomonitoring Methods, (hochregal.net)
  • Un examen rétrospectif des dossiers pré- et post-intervention a servi à décrire le séjour à l'hôpital des patients ayant reçu un diagnostic de BAC-SA avant et après la mise en place du système de diagnostic de la PCR. (cjhp-online.ca)
  • Au total, 98 et 99 patients ont satisfait au critère d'inclusion respectivement avant et après la mise en place du système de diagnostic de la PCR. (cjhp-online.ca)
  • Polymerase Chain Reaction invented a method of amplifying any DNA region through repeated cycles of implications for research applications and in forensics. (hochregal.net)
  • 17/04/2014В В· Polymerase Chain Reaction (PCR) Steps, Requirements and Applications biologyexams4u. (hochregal.net)
  • Polymerase Chain Reaction size and sequence by enzymatic method and cycling condition. (hochregal.net)
  • This powerful method has numerous applications in Theory and applications of the polymerase chain Theory and applications of the polymerase chain reaction. (hochregal.net)
  • Learn about PCR (polymerase chain reaction) a method of analyzing a short sequence of DNA or RNA. (hochregal.net)
  • Polymerase Chain Reaction Validation, Optimization, Limitations and Applications The method is useful for coding the genetic sequence of various disease, Polymerase Chain Reaction (PCR) In Medical Application: An Analytical Report 2014-2020 - The global PCR market is projected to reach around US$27.4 billion by 2015. (hochregal.net)
  • The study of Polymerase Chain Reaction (PCR) Products market is a compilation of the market of Polymerase Chain Reaction (PCR) Products broken down into its entirety on the basis of types, application, trends and opportunities, mergers and acquisitions, drivers and restraints, and a global outreach. (dsportstimes.com)
  • Along with a generalized market study, the report also consists of the risks that are often neglected when it comes to the Polymerase Chain Reaction (PCR) Products industry in a comprehensive manner. (dsportstimes.com)
  • For a global outreach, the Polymerase Chain Reaction (PCR) Products study also classifies the market into a global distribution where key market demographics are established based on the majority of the market share. (dsportstimes.com)
  • We wished to develop an accurate quantitative protocol based on a droplet digital polymerase chain reaction (ddPCR) involving eight individual TaqMan™ reactions to detect simultaneously, without selective enrichment, Listeria spp. (frontiersin.org)
  • The most sensitive method uses ligation-mediated polymerase chain reaction (LM-PCR) to amplify all fragments of the genomic sequence ladder (3,4). (nih.gov)
  • In the following we will briefly summarize our recent experience with the application of polymerase chain reaction (PCR) strategies to patients with chronic myelocytic leukemia (CML) and acute lymphoblastic leukemia (ALL) in complete remission according to clinical and laboratory parameters. (springer.com)
  • Analysis of major BCR-ABL1 mRNA by digital polymerase chain reaction is useful for prediction of international scale. (bioportfolio.com)
  • This study aimed to establish an accurate and sensitive polymerase chain reaction [‎PCR]‎ technique for the diagnosis of active human brucellosis in Egypt. (who.int)
  • Comparative Evaluation of Immunochromatographic and Reverse Transcriptase Polymerase Chain Reaction based tests for Diagnosis of Canine Distemper. (thefreelibrary.com)
  • 2014). This study is an attempt to compare polymerase chain reaction based molecular tests with commercially available IC based test for diagnosis of Canine distemper by testing rectal swabs and serum samples respectively from dogs suffering from gastroenteritis and suspected for Canine distemper. (thefreelibrary.com)
  • Development and evaluation of a real-time polymerase chain reaction for fast diagnosis of sporotrichosis caused by Sporothrix globosa. (bioportfolio.com)
  • polymerase chain reaction for fast diagnosis of sporotrichosis caused by Sporothrix globosa. (bioportfolio.com)
  • The recent development of novel Polymerase Chain Reaction (PCR) technologies that confer theoretical advantages over quantitative PCR has considerable potential in the diagnosis of low load infections. (bioportfolio.com)
  • Is Polymerase Chain Reaction in Neonatal Dried Blood Spots Reliable for the Diagnosis of Congenital Cytomegalovirus Infection? (bioportfolio.com)
  • With traditional PCR, the amount of product can only be worked out at the end of the reaction (the plateau phase), typically by agarose gel electrophoresis. (abpischools.org.uk)
  • MilliporeSigma's molecular biologists work to develop the newest, best performing polymerases for customer use, optimizing conditions, buffer compositions, and cycling parameters to save you valuable time and resources. (emdmillipore.com)
  • Polymerase chain reaction analysis of two biopsy tissues using the Cobas TaqMan revealed a positive result for Mycobacterium avium and a negative result for Mycobacterium tuberculosis . (hindawi.com)
  • However, polymerase chain reaction analysis of a cultured colony of acid-fast bacteria from biopsy tissue using the Cobas TaqMan and an alternative polymerase chain reaction analysis of biopsy tissue yielded discordant results. (hindawi.com)
  • TaqMan polymerase chain reaction was developed to quantify the number of Bifidobacterium . (ajol.info)
  • A common method of amplifying target sequences of DNA in vitro by polymerase chain reaction (PCR) is described in this simplified yet accurate animation. (merlot.org)
  • In a multiplex PCR reaction, it is possible for the different sequences to suffer from different inhibition effects to different extents, leading to disparity in their relative amplifications. (wikipedia.org)
  • Successful polymerase chain reaction (PCR) is crucial for amplifying DNA sequences in order to study their function, either by sequencing, mutation, transcription, or expression of gene products. (emdmillipore.com)
  • In Situ Polymerase Chain Reaction ( In situ PCR) is a powerful method that detects minute quantities of rare or single-copy number nucleic acid sequences in frozen or paraffin-embedded cells or tissue sections for the localization of those sequences within the cells. (biogenex.com)
  • Introduction The polymerase chain reaction (PCR) is a method that uses test tubes in biological laboratories for producing large amount of identical copies of a specific gene from small amount of complex molecules. (psu.edu)
  • CG island methylation changes near the GSTP1 gene in prostatic carcinoma cells detected using the polymerase chain reaction: a new prostate cancer biomarker. (aacrjournals.org)
  • Following cell lysis Chelex will bind to and remove the proteins and metal ions that inhibit the PCR reaction, but will leave the DNA in the solution. (ubc.ca)
  • In the present study, a genotyping method based on polymerase chain reaction (PCR) for the repeat regions of four genes (sdrG, sdrF, aap and sesE) that encode for bacterial surface proteins was developed and applied to a sample of well-characterised neonatal blood isolates of S. epidermidis (n = 49). (diva-portal.org)
  • The levels of DNA contamination are easily detectable at the sensitivity (40 fg) for the second round PCR reported by this group of authors and neither in the text nor in the figures is there any mention of a first round negative control as a test sample in the nested PCR reaction. (bmj.com)
  • It was determined whether Borrelia burgdorferi DNA may be detectable by polymerase chain reaction (PCR) in synovial membrane (SM) when PCR results from synovial fluid (SF) had become negative after antibiotic therapy. (bmj.com)
  • The most sensitive quantification methods are done by the real-time polymerase chain reaction , where the amount of DNA is measured after each cycle of PCR by use of fluorescent markers. (bionity.com)
  • DNA-based procedures are becoming increasingly common within the analytical laboratory where the polymerase chain reaction (PCR) has become an indispensable technique. (rsc.org)
  • To standardise and apply nested Polymerase Chain Reaction (n PCR) as a rapid and reliable laboratory diagnostic test to detect Toxoplasma gondii (T.gondii) in aqueous humor (AH) and peripheral blood samples of clinically suspected toxoplasma chorioretinitis patients. (arvojournals.org)
  • The polymerase chain reaction may not be exponential for the first several cycles, and furthermore, plateaus eventually, so care must be taken to measure the final amount of DNA while the reaction is still in the exponential growth phase. (bionity.com)
  • KOD polymerase yields more product in fewer cycles compared to other PCR enzymes. (emdmillipore.com)
  • With each repetition of this cycle, the target is doubled and soon, after about 30 cycles, the reaction will yield in excess of 1 million copies of the target DNA fragment. (mybiosource.com)
  • Mycobacterium tuberculosis isolates from previously treated patients (n = 88) from all regions of Syrian Arab Republic were characterized in terms of antibiotic sensitivity and genotyping using double-repetitive-element polymerase chain reaction (DRE-PCR) method for the proximity of the repetitive DNA elements IS6110 (a mobile genetic element) and PGRS. (who.int)
  • Recently, the application of the polymerase chain reaction (peR) technique developed by K. D. Mullis and detailed in the study by Saiki et al. (springer.com)
  • PCR (Polymerase chain reaction) was developed by Kary Mullis in the mid-1980s. (slideserve.com)
  • Since its introduction in the mid-80s, polymerase chain reaction (PCR) technology has been recognised as a rapid, sensitive and specific molecular diagnostic tool for the analysis of micro-organisms in clinical, environmental and food samples. (nih.gov)
  • This report presents a case of tuberculous lymphadenitis that was difficult to diagnose using polymerase chain reaction analysis. (hindawi.com)
  • Analysts at TMR estimate it to rise at a CAGR of 6.60% during the period from 2014 to 2020 and reach a value of US$9.6 bn by the end of the forecast period.The research report is titled Polymerase Chain Reaction (PCR) Market - Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020. (openbroadcast.de)
  • Ultra-rapid flow-through polymerase chain reaction microfluidics using vapor pressure. (biomedsearch.com)
  • The purpose of this study is to evaluate the test characteristics of a rapid intrapartum real- time polymerase chain reaction (RT-PCR) compared to the intrapartum GBS culture as the standa. (bioportfolio.com)