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  • samples
  • As well as methods for the removal of inhibitors from samples before PCR, some DNA polymerases offer varying resistance to different inhibitors and increasing the concentration of the chosen DNA polymerase also confers some resistance to polymerase-targeted inhibitors. (wikipedia.org)
  • copies
  • Taking into account the time it takes to change the temperature of the reaction vial, 1 million copies can be ready in about three hours. (accessexcellence.org)
  • When using dPCR, the sample is diluted and separated into a large number of reaction chambers, such that each partition contains either one or no copies of target. (sigmaaldrich.com)
  • resistance
  • Other valuable properties of the chimeric polymerases TopoTaq and PfuC2 include enhanced thermostability, specificity and resistance to contaminants and inhibitors. (wikipedia.org)
  • concentration
  • When using this technique, the sample is taken to limiting dilution and the number of positive and negative reactions is used to determine a precise measurement of target concentration. (sigmaaldrich.com)
  • Essential aspects of binding assays include, but are not limited to, the concentration level of reactants or products (see radioactive section), maintaining the equilibrium constant of reactants throughout the assay, and the reliability and validity of linked reactions. (wikipedia.org)
  • stochastic
  • However, above this size, product yields often decrease, as with increasing length stochastic effects such as premature termination by the polymerase begin to affect the efficiency of the PCR. (wikipedia.org)
  • Stochastic approaches for modelling in vivo reactions. (wikipedia.org)
  • technique
  • DNA-based procedures are becoming increasingly common within the analytical laboratory where the polymerase chain reaction (PCR) has become an indispensable technique. (rsc.org)
  • In recognition of his invention of the polymerase chain reaction (PCR) technique, he shared the 1993 Nobel Prize in Chemistry with Michael Smith and earned the Japan Prize in the same year. (wikipedia.org)
  • The Taq polymerase was heat resistant and would only need to be added once, thus making the technique dramatically more affordable and subject to automation. (wikipedia.org)
  • dPCR
  • dPCR also carries out a single reaction within a sample, however the sample is separated into a large number of partitions and the reaction is carried out in each partition individually. (wikipedia.org)
  • dPCR improves upon the current PCR practices by dividing up the reaction into multiple, smaller reactions. (wikipedia.org)
  • partitions
  • The number of reaction chambers or partitions varies between systems, from several thousand when using the QX100 Bio-Rad system to millions when using the RainDrop approach. (sigmaaldrich.com)
  • single
  • PCR carries out one reaction per single sample. (wikipedia.org)
  • Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon sizes, i.e., their base pair length, should be different enough to form distinct bands when visualized by gel electrophoresis. (wikipedia.org)