Loading...
  • RNAP
  • RNA polymerase III (RNAP III or RNA pol III) transcribes the tRNA precursors, as well as the 5S rRNA, the U6 snRNA (involved in RNA splicing )and various other small functional RNA products. (conservapedia.com)
  • Mitochondrial genes are transcribed by a separate mitochondrial RNA polymerase that is very similar in structure and function to bacterial RNAP. (conservapedia.com)
  • primer
  • This reaction is similar to that catalyzed by conventional 5′-3′ polymerases, except that the attacking oxygen nucleophile is the GTP 3′ OH moiety instead of the 3′ OH group of the elongating primer strand ( Fig. 2 ). (pnas.org)
  • yeast
  • This 3′-5′ polymerase activity also functions in vivo in yeast, but only if the tRNA His substrate is mutated to contain C73 ( 14 ). (pnas.org)
  • strand
  • In 1983, he began using two primers, one to hybridize to each strand of a target DNA, and adding DNA polymerase to the reaction. (wikipedia.org)
  • Site
  • The N-terminal domain of TFIIB brings the DNA into proper position for entry into the active site of RNA polymerase II . (wikipedia.org)
  • step
  • This G −1 addition step is a phosphodiester bond formation reaction that operates in a unique 3′-5′ direction, opposite to all conventional DNA and RNA polymerases. (pnas.org)