SynthetaseNonribosomalAcyl carrier proteinsGenesTransferaseHypotheticalCharacterizationPutativeRibosomally synthesizedAspergillusMetabolitesReductaseFatty acidEnzymeHeterologousTypeBacteriaEnzymesKinaseBiosynthesis proteinComplexIntracellularHydrolaseAcidsBacterialLyaseMethyltransferaseFamilyAliasMolecular BiologyAssembly line
Synthetase5
- Other names in common use, disregarding the synthetase/synthase spelling difference, include acyl carrier protein holoprotein synthetase, holo-ACP synthetase, coenzyme A:fatty acid synthetase apoenzyme 4'-phosphopantetheine, acyl carrier protein synthetase (ACPS), PPTase, acyl carrier protein synthase, P-pant transferase, and CoA:apo-[acyl-carrier-protein] pantetheinephosphotransferase. (wikipedia.org)
- 0001. SCO3230 CDA peptide synthetase I 3543334:3565725 forward MW:798587 0002. (kitasato-u.ac.jp)
- SCO3231 CDA peptide synthetase II 3565722:3576734 forward MW:394636 0003. (kitasato-u.ac.jp)
- SCO0492 putative peptide synthetase 513989:524920 reverse MW:389791 0004. (kitasato-u.ac.jp)
- SCO3232 CDA peptide synthetase III 3576734:3583987 forward MW:258501 0005. (kitasato-u.ac.jp)
Nonribosomal1
- Cpr37, which belongs to the adenylation domain protein in the nonribosomal peptide synthase (NRPS), subsequently activates PABA and loads it to holo-Cpr36 Two proteins Cpr34 and Cpr35 work in concert to catalyze decarboxylative condensation between a thioester linked PABA and malonyl- S -acyl carrier protein (ACP) during aromatic polyketide biosynthesis catalyzed by type II polyketide synthases. (uky.edu)
Acyl carrier proteins1
- The human 4'-phosphopantetheine transferase is also capable of phosphopantetheinylation of peptidyl carrier and acyl carrier proteins from prokaryotes. (rhea-db.org)
Genes4
- Polyketide genes in the marine sponge Plakortis simplex: a new group of mono-modular type I polyketide synthases from sponge symbionts. (cirn-na.com)
- Activation of the acyl carrier protein (ACP) domain of the SwfA protein to its holo-form by co-expression with Svp is the first functional proof of swf type genes in marine sponges. (cirn-na.com)
- strain B2 with a G+C content of 70.1 % and 4,663 candidate protein-encoding genes. (scienceopen.com)
- SMs are biosynthesized from primary metabolites in specialized pathways (i.e. polyketides or mevalonate pathways derived from Acetyl Coenzyme A, or amino acids) and some genes are clustered together. (123dok.org)
Transferase2
Hypothetical22
- tomato) conserved hypothetical protein , psesy-PSPTO2217 Pseudomonas syringae (pv. (inrae.fr)
- SCO6428 hypothetical protein 7097567:7104511 reverse MW:242150 0007. (kitasato-u.ac.jp)
- SCO6220 hypothetical protein SC2H4.02 6836057:6842608 forward MW:233391 0011. (kitasato-u.ac.jp)
- SCO6626 hypothetical protein SC1F2.23 7348537:7353210 forward MW:169206 0016. (kitasato-u.ac.jp)
- SCO6432 hypothetical protein 7111866:7116089 forward MW:148817 0021. (kitasato-u.ac.jp)
- SCO6348 hypothetical protein SC3A7.16c 7006807:7010892 reverse MW:144986 0023. (kitasato-u.ac.jp)
- SCO2975 hypothetical protein SCE50.03 3236736:3240773 forward MW:135331 0025. (kitasato-u.ac.jp)
- SCO6572 hypothetical protein SC3F9.07 7275867:7279583 forward MW:133432 0031. (kitasato-u.ac.jp)
- SCO6431 hypothetical protein 7108264:7111779 forward MW:126560 0034. (kitasato-u.ac.jp)
- SCO0072 hypothetical protein 60740:64093 reverse MW:119295 0038. (kitasato-u.ac.jp)
- SCO4250 hypothetical protein SCD8A.23 4656900:4660235 forward MW:103694 0040. (kitasato-u.ac.jp)
- SCO5271 hypothetical protein 5731309:5734599 forward MW:117582 0041. (kitasato-u.ac.jp)
- SCO6994 hypothetical protein SC8F11.20c. 7762852:7766112 reverse MW:113220 0042. (kitasato-u.ac.jp)
- SCO5717 hypothetical protein SC3C3.03c 6233478:6236729 reverse MW:112052 0044. (kitasato-u.ac.jp)
- SCO0069 hypothetical protein 56225:59383 forward MW:114192 0046. (kitasato-u.ac.jp)
- SCO0335 hypothetical protein 336333:339467 forward MW:111223 0047. (kitasato-u.ac.jp)
- SCO4331 hypothetical protein SCD12A.14 4742776:4745904 forward MW:110720 0048. (kitasato-u.ac.jp)
- SCO5842 hypothetical protein SC9B10.09 6394529:6397648 forward MW:107570 0049. (kitasato-u.ac.jp)
- SCO4548 hypothetical protein 4964060:4967068 forward MW:105802 0052. (kitasato-u.ac.jp)
- SCO0305 hypothetical protein 299213:302092 forward MW:106033 0061. (kitasato-u.ac.jp)
- SCO4254 hypothetical protein SCD8A.27 4663248:4666118 forward MW:101164 0062. (kitasato-u.ac.jp)
- SCO2116 hypothetical protein 2273596:2276427 forward MW:100732 0063. (kitasato-u.ac.jp)
Characterization3
- Here we present the functional assignment and biochemical characterization of seven proteins, Cpr33-38 and Cpr12, that initiate the biosynthesis of the polyamide. (uky.edu)
- Functional characterization of Cpr38, which has sequence similarity to the gene products encoded by pabA and pabB from E. coli , revealed that it functions as a 4-amino-4-deoxychorismate (ADC) synthase catalyzing a two-step reaction involving amidohydrolysis of L-Gln with ammonia channeled and incorporated into chorismic acid to generate ADC. (uky.edu)
- functional characterization of nat/ncs2 proteins of aspergillus brasiliensis reveals a genuine xanthine-uric acid transporter and an intrinsically misfolded polypeptide. (liverpool.ac.uk)
Putative21
- glycinea) coronatine biosynthesis protein, putative cmae , psesy-PSPTO5448 Pseudomonas syringae (pv. (inrae.fr)
- SCO0127 putative multi-domain beta keto-acyl synthase 112932:119654 forward MW:235996 0009. (kitasato-u.ac.jp)
- SCO7682 putative non-ribosomal peptide synthase 8506283:8512972 forward MW:239268 0010. (kitasato-u.ac.jp)
- SCO0126 putative multi-domain beta keto-acyl synthase 106637:112885 forward MW:214716 0012. (kitasato-u.ac.jp)
- SCO7683 putative non-ribosomal peptide synthase 8512969:8518497 forward MW:199655 0013. (kitasato-u.ac.jp)
- SCO3285 putative large glycine/alanine rich protein 3628804:3634122 reverse MW:187287 0014. (kitasato-u.ac.jp)
- SCO3869 putative WD-40 repeat protein 4252856:4257886 forward MW:177236 0015. (kitasato-u.ac.jp)
- SCO2383 putative secreted protein 2552256:2556893 forward MW:161489 0017. (kitasato-u.ac.jp)
- SCO4508 putative cell division-related protein 4928990:4933567 reverse MW:163295 0018. (kitasato-u.ac.jp)
- SCO7015 putative membrane protein. (kitasato-u.ac.jp)
- SCO5710 putative large Pro/Ala/Gly-rich protein 6221737:6225837 forward MW:141045 0022. (kitasato-u.ac.jp)
- SCO2450 putative protein kinase (regulator) 2632795:2636844 forward MW:145674 0024. (kitasato-u.ac.jp)
- SCO2259 putative multi-domain regulatory protein. (kitasato-u.ac.jp)
- SCO6219 putative ATP/GTP binding protein 6832135:6835920 forward MW:133958 0029. (kitasato-u.ac.jp)
- SCO4263 putative transcriptional regulatory protein 4675582:4679337 forward MW:132620 0030. (kitasato-u.ac.jp)
- SCO7037 putative secreted protein 7826610:7830005 forward MW:118466 0036. (kitasato-u.ac.jp)
- SCO2672 putative membrane protein 2908366:2911707 forward MW:112750 0039. (kitasato-u.ac.jp)
- SCO0369 putative secreted protein 373998:377255 reverse MW:117277 0043. (kitasato-u.ac.jp)
- SCO0372 putative secreted protein 383578:386592 reverse MW:107736 0050. (kitasato-u.ac.jp)
- SCO0505 putative multi-domain protein (fragment) 539123:542131 forward MW:108602 0051. (kitasato-u.ac.jp)
- SCO4874 putative integral membrane protein 5303718:5306603 forward MW:99145 0060. (kitasato-u.ac.jp)
Ribosomally synthesized1
- Grisemycin, salinipeptin, and cypemycin belong to the linaridin class of ribosomally synthesized and posttranslationally modified peptides that contain multiple dehydrobutyrine and D-amino acid residues. (hokudai.ac.jp)
Aspergillus1
- the filamentous fungus aspergillus oryzae is a well-known expression host used to express homologous and heterologous proteins in a number of industrial applications. (liverpool.ac.uk)
Metabolites2
- Gas chromatography MS is ideal for screening engineered bacterial strains for small metabolites like terpenes, fatty acids or small polyketides. (drexel.edu)
- This technique has frequently been applied to animal tissue slices for the purpose to map proteins, peptides , lipids, sugars or small metabolites to find disease specific bio-markers or to study drag metabolism. (go.jp)
Reductase1
- In this scenario, hydride is predicted to be added to the re face to generate the S configuration resulting in the same stereochemical outcome as other 3-oxoacyl-ACP reductase (FabG) from bacterial type II fatty acid synthases.These findings are critical advancement for interrogating the biosynthesis of the unusual chemical components of the family of antibiotics of capuramycin. (uky.edu)
Fatty acid4
- It transfers a 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to an invariant serine in an acyl carrier protein (ACP), a small protein responsible for acyl group activation in fatty acid biosynthesis. (wikipedia.org)
- The enzyme is capable of transferring the 4'-phosphopantetheine moiety of coenzyme A to a conserved serine residue in both the acyl carrier protein domain of the human cytosolic multifunctional fatty acid synthase and the acyl carrier protein associated independently with human mitochondria. (rhea-db.org)
- Screening of the metagenome of the marine sponge Plakortis simplex led to the discovery of the swf family, a new group of mono-modular type I polyketide synthase/fatty acid synthase (PKS/FAS) specifically associated with sponge symbionts. (cirn-na.com)
- These enzymes are found in a variety of biosynthetic contexts, which include fatty-acid activating enzymes, type I or type III polyketide synthases, dialkylresorcinol-generating enzymes, monooxygenases or Rieske proteins. (biomedcentral.com)
Enzyme3
- In enzymology and molecular biology, a holo-[acyl-carrier-protein] synthase (ACPS, EC 2.7.8.7) is an enzyme that catalyzes the chemical reaction: CoA-[4'-phosphopantetheine] + apo-acyl carrier protein ⇌ {\displaystyle \rightleftharpoons } adenosine 3',5'-bisphosphate + holo-acyl carrier protein This enzyme belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. (wikipedia.org)
- The systematic name of this enzyme class is CoA-[4'-phosphopantetheine]:apo-[acyl-carrier-protein] 4'-pantetheinephosphotransferase. (wikipedia.org)
- The human enzyme, which is expressed mainly in the cytosolic compartment in a wide range of tissues, is a 329-residue, monomeric protein. (rhea-db.org)
Heterologous1
- to facilitate higher yields of proteins of interest, we constructed the pasop vector to express heterologous proteins in a. oryzae. (liverpool.ac.uk)
Type2
- Acyltransferase Domain Exchange between Two Independent Type I Polyketide Synthases in the Same Producer Strain of Macrolide Antibiotics. (go.jp)
- protein_coding" "Cz05g27200.t1","No alias","Chromochloris zofingiensis","SAM-dependent methyltransferase RsmB/NOP2-type [Interproscan]. (ntu.edu.sg)
Bacteria1
- Holmqvist, E. & Vogel, J. RNA-binding proteins in bacteria. (nature.com)
Enzymes1
- All ACPS enzymes known so far are evolutionally related to each other in a single superfamily of proteins. (wikipedia.org)
Kinase1
- However, the mechanisms underpinning neuronal losses, such as in the brainstem, Protein Tyrosine Kinase inhibitor are poorly understood. (fgfrinhibitors.com)
Biosynthesis protein1
- Bifunctional purine biosynthesis protein PurH-like [Interproscan]. (ntu.edu.sg)
Complex4
- As an example of one of the complex transformations on this pathway, the figure below shows the structure of the pyrimidine synthase catalyzing the complex rearrangement of aminoimidazole ribotide (left) to the thiamin pyrimidine (right). (tamu.edu)
- Peptide epimerase-dehydratase complex responsible for biosynthesis of the linaridin class ribosomal peptides. (hokudai.ac.jp)
- In this study, we confirmed that both GrmH and GrmL, which were shown to constitute a protein complex by a co-purification experiment, were required to catalyze the dehydration, epimerization, and proteolytic cleavage of a precursor peptide GrmA by in vivo experiments. (hokudai.ac.jp)
- Furthermore, we demonstrated that GrmH/GrmL complex accepted salinipeptin and cypemycin precursor peptides, which possess three additional amino acids. (hokudai.ac.jp)
Intracellular1
- In addition to their low toxicity, gamma-AApeptides are resistant to protease degradation, which may prove to be advantageous over alpha-peptides for further development of molecular transporters for intracellular delivery. (egfrsignals.com)
Hydrolase3
- tomato) 3-oxoadipate enol-lactone hydrolase family protein , psesy-PSPTO4089 Pseudomonas syringae (pv. (inrae.fr)
- tomato) dienelactone hydrolase family protein , psesy-PSPTO2209 Pseudomonas syringae (pv. (inrae.fr)
- protein_coding" "Cz09g17180.t1","No alias","Chromochloris zofingiensis","Inosine/uridine-preferring nucleoside hydrolase domain [Interproscan]. (ntu.edu.sg)
Acids1
- Twenty alpha-amino acids are the subunits which are polymerized to form proteins. (lookformedical.com)
Bacterial3
- Bacterial colonies on an agar surface expressing a synthase that produces a deep blue dye. (drexel.edu)
- Carrier, M.-C., Lalaouna, D. & Massé, E. Broadening the definition of bacterial small RNAs: characteristics and mechanisms of action. (nature.com)
- Bacterial siderophores are small molecules with unmatched capacity to scavenge iron from proteins and the extracellular milieu, where it mostly occurs as insoluble Fe 3+ . (biomedcentral.com)
Lyase1
- Cpr12, encoded by a gene that was originally proposed to be outside the gene cluster and sharing similarity to proteins annotated as ADC lyase, was revealed to catalyze the elimination of pyruvate to form PABA. (uky.edu)
Methyltransferase1
- protein_coding" "Cz08g18150.t1","No alias","Chromochloris zofingiensis","Class I-like SAM-dependent O-methyltransferase [Interproscan]. (ntu.edu.sg)
Family2
Alias1
- protein_coding" "Cz01g18210.t1","No alias","Chromochloris zofingiensis","DNA recombination and repair protein RecA [Interproscan]. (ntu.edu.sg)
Molecular Biology1
- Our research involves a combination of molecular biology, protein biochemistry, organic synthesis and structural studies and provides a strong training for students interested in understanding the organic chemistry of living systems and in pursuing careers in biotechnology, drug design or academia. (tamu.edu)
Assembly line1
- Biosynthetic gene cluster (BGC) of vancomycin, highlighting the conveyor-belt assembly line of this non-ribosomal peptide synthase producing the important antibiotic vancomycin, produced in Amycolatopsis orientalis. (drexel.edu)