• To solve these problems, an amphiphilic polymer-drug conjugate was prepared by attachment of low molecular weight (ie, 2 kDa) methoxy poly(ethylene glycol) methyl ether (mPEG) to gambogic acid (GA-mPEG 2000 ) through an ester linkage and characterized by 1H nuclear magnetic resonance. (dovepress.com)
  • The probe was a conjugate of monomethyl-poly-(ethylene glycol)-5000, 1-naphthyl-ethylenediamine and biotin. (uky.edu)
  • In contrast, the equilibrium constant for distearoylphosphatidylethanolamine-poly(ethylene glycol) (DSPE-PEG5k) was 0.4±0.1 (mg/m 2 )/(mg/ml). (princeton.edu)
  • In contrast, the equilibrium constant for distearoylphosphatidylethanolamine-poly(ethylene glycol) (DSPE-PEG5k) was 0.4±0.1 (mg/m2)/(mg/ml). (princeton.edu)
  • Polyethylene glycols (PEGs) and their derivatives are non-ionic polymers of ethylene oxide commercially available with numerous synonyms, such as macrogol, oxyethylene polymer, and laureth-9. (biomedcentral.com)
  • Polyethylene glycols (PEGs) and their derivatives are non-ionic polymers of ethylene oxide commercially available over a wide range of molecular weights from 200 g/mol to 35,000 g/mol and widely used in medical, pharmaceutical, cosmetic, industrial, and food products [ 1 , 2 ]. (biomedcentral.com)
  • Blood tests such as enzyme-linked immunosorbent assay (ELISA) and immunofluorescence are routinely performed to detect anti-dsDNA antibodies in diagnostic laboratories. (wikipedia.org)
  • The serum was transferred into cryotubes and frozen at -80°C so that samples would arrive at IMTSSA for analysis still frozen (-20°C). Each sample was systematically tested for RVFV-specific immunoglobulin (Ig) G by using an enzyme-linked immunosorbent assay (ELISA). (cdc.gov)
  • Hybridomas were then screened in a solid-phase indirect enzyme-linked immunosorbent assay (ELISA) against 40:1 4,4'-MDI-human serum albumin (HSA) and 4,4'-MDI-keratin (KER) protein conjugates. (cdc.gov)
  • Enzyme-linked immunosorbent assays (ELISA) have been around as one of the primary methods of analyte detection for more than four decades. (ddw-online.com)
  • Over the years many changes to the basic format have resulted in assay improvements, but some of the most recent look set to take the traditional ELISA to new levels. (ddw-online.com)
  • These include many vendors now addressing the automation of ELISA assays. (ddw-online.com)
  • Other automated instrumentation is designed to support the creation/coating of ELISA assay plates or contains cartridge-based delivery of assay reagents to reduce set-up time. (ddw-online.com)
  • These are supported by a wide range of ELISA reagents, assay and kits providing relative profiling or absolute quantitation of proteins across a wide range of study areas. (ddw-online.com)
  • E nzyme-linked immunosorbent assays (ELISA) have been one of the primary methods of detecting the presence of a substance, usually an antigen, in an aqueous sample for more than 40 years. (ddw-online.com)
  • The immunoassay landscape has, however, dramatically changed in recent years with the development of reduced assay steps protocols, no wash ELISAs and new automated systems designed to run ELISA. (ddw-online.com)
  • This review article uses as its starting point some of the findings of HTStec's ELISA Assay Trends 2012 survey and report, published in June 2012 (1). (ddw-online.com)
  • This report discusses end-users' current practices and preferences in ELISA assays, and seeks to understand future user requirements. (ddw-online.com)
  • The main (primary) application area for survey respondents' ELISA assays was immunology (17% selecting). (ddw-online.com)
  • Myeloma cells were added to spleen cells at a 5:1 myeloma/spleen cell ratio, and the cells in the mixed suspension were fused using a 50% solution of polyethylene glycol (1450 molecular weight) at 37°C. On day ten screening for hybrids secreting antibodies was performed using an enzyme linked immunosorbent assay (ELISA). (vin.com)
  • The enzyme-linked immunosorbent assay (or ELISA as it's extra generally recognized) was developed within the 1970s and took the place of the radioimmunoassay approach that had been generally used up till that point. (populationhealthsciences.org)
  • For example, Creative Diagnostics supplies High Sensitivity Polyethylene Glycol (PEG) ELISA Kit (Catalog # DEIA6158) for research applications, which can be used to detect proteins containing one or more PEG chains as well as unconjugated PEG. (jacksonnewsreporter.com)
  • The enzyme-linked immunosorbent assay testing technique has a lot to supply . (populationhealthsciences.org)
  • Individual and pooled donations are tested for transfusion-transmissible infections, including hepatitis B virus, hepatitis C virus, human immunodeficiency virus, parvovirus B19, and hepatitis A virus, by using enzyme-linked immunosorbent assays or nucleic acid amplification technologies. (ingentaconnect.com)
  • Techniques for measuring protein S antigen include enzyme-linked immunosorbent assays (ELISAs) and radioimmunoassays. (medscape.com)
  • The method uses sulfated alpha-cyclodextrin in the presence of Mg +2 , which forms complexes with apoB containing lipoproteins, and polyethylene glycol-coupled cholesteryl esterase and cholesterol oxidase for the HDL-cholesterol measurement. (cdc.gov)
  • HDL cholesterol was measured by a direct non-precipitating method using polyethylene glycol-coupled cholesteryl esterase (PEG-modified enzyme) and cholesterol oxidase. (who.int)
  • Preliminary Results of a Local Lymph Node Assay and a 28-Day Repeated-Dose Toxicity Study in Rats with Fatty Acids, C-18-Unsaturated, Dimers, Polymers with Tall-oil Fatty Acids, Tetraethylenepentamine and Triethylenetetramine, Compounds with C18-Unsaturated, Fatty Acid Trimers. (epa.gov)
  • PEGs by Utility Polyethylene glycol(PEG) derivatives are utilized in a lot of fields, along with medical evaluation, drug-release, nanotechnology, new provides evaluation, cell custom, and totally different functions. (accenx.com)
  • Creative Diagnostics is a leading manufacturer and supplier of antibodies, viral antigens, innovative diagnostic components, and critical assay reagents. (jacksonnewsreporter.com)
  • The purpose of this review is to provide a complete update of the various analytical methods for virus detection, such as molecular, immunological, sensor-based and other detection assays, along with the advantages and limitations of these strategies. (frontiersin.org)
  • Protein S assays can be either functional or immunological. (medscape.com)
  • Immunological assays measure total protein S, both free protein S and bound protein S. In rare circumstances, the level of immunological protein S is normal but some functional abnormality exists (ie, a true type 2 deficiency). (medscape.com)
  • Assays for folate, theophylline, and tobramycin (using such labels with hydrophilic modifiers such as nonionic polyethylene glycol and polyionic spermine disulfonate and polyionic spermine dicarboxylate) are described in detail. (justia.com)
  • In this study, the properties of CAB nanofibers were improved by fabricating the composite nanofibers made of CAB and hydrophilic polyethylene glycol (PEG). (sunway.edu.my)
  • Total cholesterol, HDL-cholesterol and triglyceride concentrations were measured in serum by a Roch/Hitachi Modular P Chemistry Analyzer (Mod P). The cholesterol assay used the cholesterol oxidase/peroxidase (CHOD/POD) method. (who.int)
  • The antigen used was a precipitate (polyethylene glycol 6000) of the supernatant of Vero cells infected with the RVFV clone 13. (cdc.gov)
  • Lipid peroxidation assay protocol summary: - add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min - swap to wells of microplate - analyze with microplate reader For higher sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet sooner than analysis. (accenx.com)
  • For detecting free protein S, the C4bBP-bound protein S is removed from the assay by a pre-assay step involving precipitation of the C4bBP-bound protein S with polyethylene glycol and then using centrifugation to remove the precipitate. (medscape.com)
  • We present results on using cooperative interactions to shield liposomes by incorporating multiple hydrophobic anchoring sites on polyethylene glycol (PEG) polymers. (princeton.edu)
  • In addition to chromatography steps, the manufacturing process consists of dedicated and effective pathogen reduction steps, including pasteurization, hydrophobic interaction chromatography or polyethylene glycol precipitation, and virus filtration. (ingentaconnect.com)
  • Bacterial Endotoxins Test describes the procedures for performing Limulus amebocyte lysate (LAL) assays for the detection of endotoxins in pharmaceutical products. (pharmaceuticalonline.com)
  • P erhaps most significantly are the big increases in assay sensitivity and the widening of the dynamic range accomplished by using alternatives to the traditional colorimetric detection, or by the use of new platform technologies. (ddw-online.com)
  • The finished product specifications include tests for description, identification of the APIs (HPLC and TLC), water content, average mass, uniformity of dosage units (by content uniformity), dissolution, related compounds (HPLC), assay (HPLC), microbiological examination and XRPD (for detection of API crystalline forms). (who.int)
  • After immediate spin, the tubes are incubated at 37°C. To promote the detection of warm reactive antibodies, especially of the IgG class, additional enhancement techniques such as low ionic strength saline (LISS) and polyethylene glycol (PEG) are often used. (medscape.com)
  • Using in vitro(31)P and C-13 nuclear magnetic resonance (NMR) spectroscopy and biochemical assays, we analysed metabolite profiles of perchloric extracts from germinating radicles of cucumber to identify changes in carbon and phosphate metabolism associated with DT. (inrae.fr)
  • Serum total T4 and T3 assays measure both bound and free (unbound) hormone. (medscape.com)
  • Poly(ADP ribose) polymerase 1 (PARP1) is a multifunctional DNA repair protein of the base excision repair pathway and plays a major role in the repair of DNA strand breaks and in replication and transcriptional regulation among other functions. (nist.gov)
  • Functional assays measure only free (physiologically active) protein S. Functional assays are usually based on either the prothrombin time (PT) or the activated partial thromboplastin time (aPTT). (medscape.com)
  • With aPTT-based functional protein S assays, platelet-poor plasma is incubated at 37°C with the following: protein S-deficient plasma, phospholipid, a contact activator (eg, Kaolin), and either an excess of activated protein C or an activator of protein C .Typically, the incubation period is 1-4 minutes, after which calcium is added to initiate clotting. (medscape.com)
  • For PT-based functional protein S assays, the activated protein C cofactor activity of protein S is measured based on the degree of prolongation of a PT-based clotting time. (medscape.com)
  • A membrane receptor reagent and assay is disclosed in which liposomes are bound to an evanescent wave emitting surface. (google.com)
  • 1,2-distearoyl-sn-glycero-3-phospho-ethanolamine-N-(methoxy[polyethyleneglycol]-2000) (DSPE-PEG2000) has been widely used in the preparation of stealth liposomes. (mendeley.com)
  • The initial assay was also briefly optimized for speed and sensitivity. (9pdf.co)
  • The developed singleplex assay as singleplex defeated a reference PCR method at speed, whereas at sensitivity it was lacking. (9pdf.co)
  • The results show that polyethylene glycol (PEG) modified black phosphorus crystals (BP) nanoparticles can effectively improve their negative electricity, and have relatively stable photothermal properties. (frontiersin.org)
  • The cytotoxicity of PTX-loaded LPP2000 was also higher than that of LDP2000 and LPP5000 as observed using a WST-8 assay, while blank LPPs showed negligible toxicity. (mendeley.com)
  • Second, a study undertaken by the Pasteur Institute of Paris in collaboration with the EMVT (Département Elevage et Médecine Vétérinaire Tropicale du Centre de Coopération Internationale pour la Recherche en Agronomie pour le Développement) showed that 4% of sheep bred in Chad and Ethiopia had anti-RVFV neutralizing antibodies ( 4 ). (cdc.gov)
  • 9 While this alternative assay seemed to be a very practical solution, several challenges to assuring reproducible results were evident. (pharmaceuticalonline.com)
  • The cell viability assay results revealed that the nanofibers are non-toxic. (sunway.edu.my)
  • We tested the 2,4,5-trimethyl-2,5-dihydro-1,3-thiazole in our assay and the results look good. (polyethyleneglycolpeg.com)
  • Polyethylene glycol (PEG) is a classic non-scaling water-soluble synthetic polymer widely used in the pharmaceutical field for proteins and drug carriers. (jacksonnewsreporter.com)
  • Emerged radicles measuring 2 mm long can be rendered tolerant to desiccation by incubation in a polyethylene glycol (PEG) solution with a water potential of -1.5 MPa. (inrae.fr)
  • Water samples were assayed for potential respiratory viruses (adenoviruses and enteroviruses), but none were detected. (cdc.gov)
  • Cytotoxicities of paclitaxel niosom polyethyleneglycol, paclitaxel niosome and free paclitaxel on MCF-7 cell line after 48 hours were studied by MTT assay. (scialert.net)
  • Following 6 biweekly immunizations, splenocytes were recovered 72 h after the final immunization and fused to Sp2/0-Ag14 myeloma cell line using 50% polyethylene glycol (1500 MW) for hybridoma production. (cdc.gov)
  • These updates were reflected in the withdrawal of the 1987 FDA guidance and publication in 2012 of a question and answer (Q&A) document intended to explain how to use the LAL assay in support of patient safety assays for product release and process development. (pharmaceuticalonline.com)
  • This article reviews the development and evolution of the LAL assay, its uses, and misunderstandings that have occurred. (pharmaceuticalonline.com)
  • It resulted in development of candidate duplexed Yersinia SIBA® assay with internal control. (9pdf.co)
  • In addition to providing contract biologic R&D and manufacturing services for diagnostic manufacturers along with GMP biologics manufacturing for the biopharmaceutical market, the company aims to provide a trusted source for all researchers' assay development and manufacturing needs. (jacksonnewsreporter.com)
  • assay by using oligo screening method. (9pdf.co)
  • The U.S. Food and Drug Administration (FDA) has established regulations for pyrogenicity of drug products, and had provided guidance relating to the use of the LAL assay as an indicator of pyrogenicity in lieu of the rabbit pyrogenicity test. (pharmaceuticalonline.com)
  • and to processes for performing bioanalytical assays for target analytes which use such labels. (justia.com)
  • GNPs were functionalized with Polyethylene glycol (PEG) and RGD peptide to specifically target cancerous cells. (aapm.org)
  • These criteria were tested for a competitive bioaffinity assay of biotin. (uky.edu)
  • These proposed criteria may be universally applicable for linear responses in competitive assays of ligands. (uky.edu)
  • Presence of polyvinyl polypyrrolidone (PVP) or polyethylene glycol (PEG) 4000 was indispensable for PAL activity in all the tested methods of enzyme extraction. (ajevonline.org)
  • The study included 56 individuals with obesity, of whom 30 individuals underwent Roux-en-Y gastric bypass surgery (RYGB) and twenty-six underwent sleeve gastrectomy (SG). (biomedcentral.com)
  • Included also is an exposure to polyethylene (PE) study because PE metabolizes to PO. (who.int)
  • Therefore, based on the characteristics of black phosphorus two-dimensional nanomedicine, experimental designs were conducted on its nanosheet preparation, polyethylene glycol modification, and anti-tumor drug loading. (frontiersin.org)
  • Developed singleplex and duplex Yersinia assays were also shortly optimized by adjusting one parameter at the time. (9pdf.co)