Polyclonal antibody epitope. Medical search. Frequent questions

Polyclonal or monoclonal antibody technology offers different advantages that are desirable for specific applications. (sigmaaldrich.com)
With monoclonal antibody production, one B cell produces only one specific sequence of antibody to a given antigen. (sigmaaldrich.com)
Hence, a monoclonal antibody is considered a collection of identical antibodies secreted by a single B cell clone with specificity for only one antigenic epitope. (sigmaaldrich.com)
However, expression of these two chains on separate vectors is not an efficient way to generate monoclonal antibody producing cell lines. (sigmaaldrich.com)
Monoclonal antibody raised in mouse against the N-terminus of the Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein. (diagenode.com)
Alternative name: Csn1 Monoclonal antibody raised in mouse against the N-terminus of the Cas9 nuclease (CRISPR-associated protein 9) using a recom. (diagenode.com)
Other names: m1A, 1mA, m1Ado Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH. (diagenode.com)
Monoclonal antibody raised in mouse against 6X His epitope tag, using a KLH-conjugated synthetic peptide. (diagenode.com)
Moreover, as differences in monoclonal antibody reactivity was found between the two assays, monoclonal antibodies always should be selected by an assay mimicking the desired use of the final antibodies as closely as possible. (ku.dk)
human monoclonal antibody (b96.11 huAb) and polyclonal antibodies (SMS patients' sera) were investigated. (nottingham.ac.uk)
We isolated an MBC-derived GII.17-specific Immunoglobulin A (IgA) monoclonal antibody called NVA.1 that potently and selectively blocked GII.17 cluster IIIb and recognized an epitope targeted in serum from cluster IIIb-infected children. (cdc.gov)

Unlike monoclonal antibodies produced by hybridoma technology, recombinant antibodies maintain a high degree of specificity and low immunogenicity over longer periods of time. (sigmaaldrich.com)
Antibodies with specificity for native and denatured forms of ovalbumin differ in reactivity between enzyme-linked immunosorbent assays. (ku.dk)
These antibodies were characterized with respect to reactivity to native and denatured OVA by enzyme-linked immunosorbent assay (ELISA) employing surface-bound OVA and streptavidin-capture ELISA to determine whether effects of different coating influence antibody specificity and with respect to epitope specificity by peptide ELISA, using overlapping peptides, covering the complete OVA sequence. (ku.dk)
At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.com)
The fine specificity, as determined by the requirement of particular amino acid residues in the epitope, is shifted in these new antibody fragments. (lu.se)
Polyclonal rabbit antibodies produced against the naphthyl-modified keratins reacted with their respective antigens with threshold sensitivities of 15-31 ng/mL and high specificity over a linear range up to 500 ng/mL. (cdc.gov)

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (origene.com)
639-abx023906 Species Reactivity: Chemical Host/Isotype: Rabbit / IgG Class: Recombinant Monoclonal Type: Antibody Clone: RM3 Immunogen: 25-OH Vitamin D3 BSA. (anobase.org)
665-STJ90151-50UL Species Reactivity: Human Host/Isotype: Rabbit / IgG Class: Recombinant Monoclonal Type: Antibody Clone: 10 H13L14 Immunogen: Peptide. (anobase.org)

Primary Antibodies are guaranteed for 1 year from date of receipt. (novusbio.com)
Rabbit, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. (gentaur.com)

Moreover, comparison of antibody reactivity to N OVA revealed that in the streptavidin-capture ELISA, antibody reactivity was notably reduced compared to ELISA employing surface-bound OVA. (ku.dk)
To further define the N-terminal MoAb epitope, sequencing of N-terminal MoAbs reactive peptides which were obtained from the successful biopanning using M13 gene VIII 5C4C4 phage Iibrary and were selected by high affinity binding in immnuo-blotting assay using nitro-cellulose membrane and in capture ELISA. (nottingham.ac.uk)
Thus, the overall motif or GAD-6 may correspond to 523-528 aa of GAD-65 (M-S-R-L-S-K). To further define the GAD-6 epitope, sequencing or GAD-6 reactive peptides which were obtained from the successful biopanning using MI3 gene III C7C and linear 12-mers phage libraries, did not show a clear motif and did not show reactivity with GAD-6 by capture ELISA. (nottingham.ac.uk)
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. (gentaur.com)
Depending on the epitopes used human ELISA kits can be cross reactive to many other species. (gentaur.com)
Des préparations d'antigènes bruts de Setaria equina ont été utilisées dans le cadre des méthodes ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
This Cdc25C phospho Ser216) antibody is for WB, IHC-P, ELISA. (bd-ibr.org)
This Cdc25A phospho Thr507) antibody is for WB, ELISA. (bd-ibr.org)
Epitope Elisa Laboratories manufactures the elisa epitope tagged antibody reagents distributed by Genprice. (taq-polymerase.net)
The Elisa Epitope Tagged Antibody reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (taq-polymerase.net)
The following synthetic S-arylcysteines were incorporated into 10-mer head sequences of K1 [GGGRFSS(S-aryl-C)GG] and K10 [GGGG(S-aryl-C)GGGGG] to form the predicted immunogenic epitopes for antibody production for ELISA: S-phenylcysteine-K1 (SPK1), S-phenylcysteine-K10 (SPK10), S-(1-naphthyl)cysteine-K1 (1NK1), S-(1-naphthyl)cysteine-K10 (1NK10), S-(2-naphthyl)cysteine-K1 (2NK1), and S-(2-naphthyl)cysteine-K10 (2NK10). (cdc.gov)
Anti-S-phenylcysteine antibodies were not sufficiently specific or sensitive toward the target antigens for use in ELISA under our experimental conditions. (cdc.gov)

Epitope mapping revealed that a majority of the polyclonal antibodies recognized multiple linear epitopes, and based on these results, a semi-automated method was developed for peptide enrichment using polyclonal antibodies immobilized on protein A-coated magnetic beads. (nih.gov)
A protocol based on the simultaneous multiplex capture of more than 40 protein targets showed that approximately half of the antibodies enriched at least one functional peptide detected in the subsequent mass spectrometry analysis. (nih.gov)
As a result, the DDDDK peptide is more likely to be accessible to antibodies. (ptglab.com)
498-BS-7588R Rabbit polyclonal to A4GALT Suitable for: IHC-P Reacts with: Human Isotype: IgG Synthetic peptide within Human A4GALT aa 250-350 conjugated to keyhole limpet. (anobase.org)
Polyclonal antibody raised in rabbit against CEBP Alpha (CCAAT/enhancer binding protein (C/EBP), alpha), using a KLH-conjugated synthetic peptide. (diagenode.com)
Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein1. (diagenode.com)
Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain Helicase DNA Binding Protein 1), using a synthetic peptide containing a sequence from t. (diagenode.com)
These precise epitope investigations were performed using two different phage-displayed random peptide libraries with different characteristics (T7 phage library gene X C9C and linear 9-mers, and M13 filamentous phage library gene III C7C and linear 12-mers and gene VIII 5C4C4). (nottingham.ac.uk)
A neutralization test in vitro showed that the peptide-specific mouse antisera of IDE4 (242-256 aa) and HN341-355, a reported neutralizing linear epitope, could partially neutralize avirulent LaSota as well as virulent strains at similar levels, suggesting that IDE4 might be a potential neutralizing linear epitope. (biomedcentral.com)
Among the methods for identifying epitopes, peptide scanning technology, such as pepscan, has been widely used due to its high sensitivity and rapidity [ 19 ]. (biomedcentral.com)
Another object of the present invention is to provide a fusion anti-tyrosinase antibody or an antigen-binding fragment thereof in which a TAT peptide is further bound to the anti-tyrosinase antibody or antigen-binding fragment thereof. (justia.com)
In addition, the present invention provides a fusion anti-tyrosinase antibody or an antigen-binding fragment thereof in which a TAT peptide represented by SEQ ID NO: 7 is further bound to the antibody or antigen-binding fragment thereof. (justia.com)
Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. (novusbio.com)
Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. (gentaur.com)
Here, we report using implantable osmotic pumps to continuously deliver a series of epitope-targeted immunogens to rhesus macaques over the course of six months to elicit immune responses against the conserved fusion peptide. (bvsalud.org)
Plasma cells produce antibodies (eg, rheumatoid factor [RF], anticyclic citrullinated peptide [anti-CCP] antibody) that contribute to these complexes, but destructive arthritis can occur in their absence. (msdmanuals.com)

Monoclonal antibodies to denatured OVA showed reactivity to both OVA forms. (ku.dk)
Two of these monoclonal antibodies, HYB 94-06 and 94-07, showed reactivity to overlapping peptides and their epitopes were identified as flexible structures constituting amino acids 130-135 and 136-141, respectively. (ku.dk)
We show here that the reactivity of a high affinity, virus-neutralizing human antibody against the AD-2 epitope of cytomegalovirus gB can be modified by introducing other Vkappa sequences together with the original VH sequence. (lu.se)

The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. (ptglab.com)
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)

It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. (gentaur.com)

Here, we describe a new method (immuno-SILAC) for the absolute quantification of proteins in complex samples based on polyclonal antibodies and stable isotope-labeled recombinant protein fragments to allow affinity enrichment prior to mass spectrometry analysis and accurate quantification. (nih.gov)
We took advantage of the antibody resources publicly available from the Human Protein Atlas project covering more than 80% of all human protein-coding genes. (nih.gov)
The results suggest that polyclonal antibodies generated via immunization of recombinant protein fragments could be used for the enrichment of target peptides to allow for rapid mass spectrometry analysis taking advantage of a substantial reduction in sample complexity. (nih.gov)
In biochemistry, immunostaining is any use of an antibody-based method to detect a specific protein in a sample. (wikipedia.org)
A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. (ptglab.com)
These eukaryotic systems offer additional advantages of protein folding, post-translational modifications, and a secretion apparatus that enhances the secretory production of antibodies. (sigmaaldrich.com)
TBPL2 Polyclonal Antibody detects endogenous levels of TBPL2 protein. (gbiosciences.com)
Polyclonal antibody raised in rabbit against the Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein. (diagenode.com)
Polyclonal antibody raised in rabbit against Lachnospiraceae bacterium (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein. (diagenode.com)
Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein. (diagenode.com)
An antibody ( Ab ), also known as an immunoglobulin ( Ig ), [1] is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses . (wikipedia.org)
The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) is a major antigen that can induce protective antibodies in poultry. (biomedcentral.com)
In this study, we analyzed B-cell immunodominant epitopes (IDEs) of the HN protein from the vaccine strain LaSota using pepscan technology with LaSota-specific chicken hyperimmune antisera. (biomedcentral.com)
The KT3 epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. (novusbio.com)
Transfection experiments with epitope-tagged hPop4 protein indicated that hPop4 is localised in the nucleus and accumulates in the nucleolus. (embl-heidelberg.de)
Immunoprecipitation assays using extracts from transfected cells expressing epitope-tagged hPop4 revealed that this protein is associated with both the human RNase MRP and RNase P particles. (embl-heidelberg.de)
Polyclonal rabbit antibodies raised against recombinant hPop4 recognised a 30 kDa protein in total HeLa cell extracts and specifically co-immunoprecipitated the RNA components of the RNase MRP and RNase P complexes. (embl-heidelberg.de)
This antibody can be antigen purified or protein A or G purified. (gentaur.com)
In a recent report, a subset of patients with limbic encephalitis associated with a systemic neoplasm previously attributed to antibodies against voltage gated potassium channel antibodies actually recognize LGI1 protein complex epitopes and do not represent a channelopathy. (medscape.com)

Multiple conformation- or misfolded-specific antibodies for human SOD1 were compared. (nature.com)

This can be accomplished by heating for varying lengths of times (heat induced epitope retrieval or HIER) or using enzyme digestion (proteolytic induced epitope retrieval or PIER). (wikipedia.org)
PRAS40 was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human PRAS40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6408) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013 ). (rndsystems.com)

Here, we show that we were able to use small amounts of antibodies (50 ng per target) in this manner for efficient multiplex analysis of quantitative levels of proteins in a human HeLa cell lysate. (nih.gov)
The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag. (caslab.com)
Antibodies have become one of the most important tools in life science research, allowing the detection, quantitation, and determination of changes in proteins and other molecules with respect to time and other perturbations. (sigmaaldrich.com)
Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. (vwr.com)
Antibodies are heavy (~150 k Da ) proteins of about 10 nm in size, [7] arranged in three globular regions that roughly form a Y shape. (wikipedia.org)
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. (gentaur.com)
These antibodies react with a group of 35- to 40-kilodalton neuronal RNA-binding proteins, including HuD[4] , PLE21/HuC, and Hel-N1. (medscape.com)
Anti-Hu antibodies may alter the production of these proteins, which are essential for the development, maturation, and maintenance of the vertebrate nervous system. (medscape.com)

Therefore, defining the linear epitopes of HN, especially neutralizing epitopes, will be useful for revealing its antigenic characterization. (biomedcentral.com)

AMPA receptor variants were identified with a polyclonal antibody recognizing the conserved extracellular loop region of all four AMPA receptor subunits (GluR1-4, both flip and flop ), whereas NMDA receptors were immunolabeled with a polyclonal antibody that binds to an extracellular N-terminal epitope of the NR1 subunit, common to all splice variants. (jneurosci.org)
After an antigen binds to a BCR, the B cell activates to proliferate and differentiate into either plasma cells , which secrete soluble antibodies with the same paratope, or memory B cells , which survive in the body to enable long-lasting immunity to the antigen. (wikipedia.org)

Rabbit antibodies are very stable and can be stored for several days at room temperature. (gentaur.com)

Hence, they are considered a mixture of antibodies that can react with multiple epitopes on the surface of the antigen. (sigmaaldrich.com)
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. (abcam.com)
Polyclonal antibodies to native OVA reacted strongly with native and denatured OVA in both assays, but did not react with the overlapping peptides. (ku.dk)

To facilitate prospective surveillance for such evolution, we map how convalescent serum antibodies are impacted by all mutations to the spike's receptor-binding domain (RBD), the main target of serum neutralizing activity. (biorxiv.org)
Binding by polyclonal serum antibodies is affected by mutations in three main epitopes in the RBD, but there is substantial variation in the impact of mutations both among individuals and within the same individual over time. (biorxiv.org)
However, a limitation of these studies is that they characterize an incomplete subset of all possible mutations, and thus do not completely describe the effects of viral mutations on recognition by polyclonal serum antibodies. (biorxiv.org)
Our complete maps of how mutations impact serum antibody binding identify three major epitopes in the RBD. (biorxiv.org)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
They represent 70% or more of serum antibodies. (gentaur.com)
Polyclonal serum antibodies bound multiple clusters but showed cluster-specific blockade activity in a surrogate virus neutralization assay. (cdc.gov)

Two affinity purified goat polyclonal antibodies have been selected to detect epitopes within the amino-terminal and carboxyl-terminal regions of mouse FGF-23. (quidel.com)
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. (gentaur.com)

However, its antigenic epitopes have not been fully elucidated. (biomedcentral.com)
These results will help elucidate the antigenic epitopes of the HN and facilitate the development of NDV vaccines. (biomedcentral.com)
However, the linear antigenic epitopes of HN have not been fully elucidated. (biomedcentral.com)
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)

Collectively, immunization with native OVA preferentially generates highly specific antibodies reacting with three-dimensional epitopes, whereas immunization with denatured OVA generates antibodies occasionally reacting with continuous epitopes. (ku.dk)
High titres of HIV-neutralizing antibodies were generated after a single booster immunization. (bvsalud.org)
A long-prime, slow-delivery (12 days) immunization approach holds promise for difficult vaccine targets and suggests that patience can have great value for tuning of germinal centres to maximize antibody responses. (bvsalud.org)

In contrast, recombinant antibodies are monoclonal antibodies that are generated by cloning antibody genes into expression vectors and do not involve the use of hybridomas. (sigmaaldrich.com)
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)

With this technology, the problems of cell line drift, antibody expression variations, and antibody sequence mutations associated with classical hybridoma production and storage can be avoided. (sigmaaldrich.com)
Therefore, the M13 pVIlI 5C4C4 worked with N-terminal MoAb by expressing a relevant sequence for the N-terminal MoAb but which is unlike its epitope in GAD-65. (nottingham.ac.uk)
The recognized KT3 epitope represents the amino acid sequence KPPTPPPEPET derived from the Simian Virus 40 (SV40) large T-antigen. (novusbio.com)

The evolution of SARS-CoV-2 could impair recognition of the virus by human antibody-mediated immunity. (biorxiv.org)
To monitor for similar antigenic evolution of SARS-CoV-2, it is important to determine which viral mutations impact human polyclonal antibody immunity. (biorxiv.org)
Because these fluids were traditionally known as humors , antibody-mediated immunity is sometimes known as, or considered a part of, humoral immunity . (wikipedia.org)
Norovirus Infection in Young Nicaraguan Children Induces Durable and Genotype-Specific Antibody Immunity. (cdc.gov)

When compared to mAbs (monoclonal antibodies) pAbs were found to have significantly lower levels of concentration. (prospecbio.com)
In this work, we evaluated a panel of 16 anti-BoNT/A monoclonal antibodies (mAbs) for their ability to inhibit the in vitro activity of BoNT/A1, /A2, and /A3 complex as well as the recombinant LC of A1. (cdc.gov)

To allow the immune system to recognize millions of different antigens, the antigen-binding sites at both tips of the antibody come in an equally wide variety. (wikipedia.org)
Cross-reaction was found between S. equina antigens and antibodies in the sera of Wuchereria bancrofti-infected patients, with the highest levels observed between sera of chronic infected patients and Setaria spp. (who.int)
We used a surrogate neutralization assay that measured antibodies blocking the binding of 13 different norovirus virus-like particles (VLPs) to histo-blood group antigens (HBGAs) in pre- and post-infection sera. (cdc.gov)
A genetic predisposition has been identified and, in White populations, localized to a shared epitope in the HLA-DRB1 locus of class II histocompatibility antigens. (msdmanuals.com)

They do this by identifying different epitopes on a given antigen. (prospecbio.com)
However, the fact that these antibodies can identify various epitopes on a given antigen creates a plethora of further advantages. (prospecbio.com)

The possibility of building up a proteome-wide resource for immuno-SILAC assays based on publicly available antibody resources is discussed. (nih.gov)

The following product was used in this experiment: SSB1 Polyclonal Antibody from Thermo Fisher Scientific, catalog # A301-938A-T. (thermofisher.com)

In this study, the precise epitopes in GAD-65 of three different mouse monoclonal antibodies (MoAbs) (N-terminal MoAb within amino acid residues 4-17. (nottingham.ac.uk)

It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. (bd-ibr.org)

Here we comprehensively map how all amino-acid mutations to the SARS-CoV-2 spike RBD affect binding by the antibodies in plasma collected from convalescent individuals ~1 to ~3 months post-symptom onset. (biorxiv.org)
The amino-terminal antibody is biotinylated for capture and the carboxyl-terminal antibody is conjugated with the enzyme horseradish peroxidase (HRP) for detection. (quidel.com)

Antibody specificities and GC responses were tracked longitudinally using electron microscopy polyclonal epitope mapping (EMPEM) and lymph node fine-needle aspirates, respectively. (bvsalud.org)

The IgG antibody has 2 antigen binding sites. (gentaur.com)

Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for human tissues. (thermofisher.com)
Epitope retrieval with citrate buffer pH 6.0 is recommended for mouse tissues. (thermofisher.com)

The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. (abcam.com)

We have developed antibodies that recognize all AMPA or all NMDA receptor variants on the surface of living neurons. (jneurosci.org)
Antibodies that recognize extracellular epitopes and can thus label iGluRs on living neurons are important tools for the study of receptor localization and dynamics. (jneurosci.org)
Memory B cells generated under these long priming conditions had higher levels of antibody somatic hypermutation, and both memory B cells and antibodies were more likely to recognize non-immunodominant epitopes. (bvsalud.org)

The five IDEs could elicit specific antibodies in mice. (biomedcentral.com)

This platform acquired its unique name because of its proprietary B cell immortalization technology that also allows us to produce monoclonal antibodies in a wide range of species. (sigmaaldrich.com)
These antibodies can be cloned from any species using suitable oligonucleotide primers. (sigmaaldrich.com)
Recombinant antibody production from most species consist of a heavy and a light chain and it is important to note that to accomplish maximum IgG production in mammalian cells, one must achieve a balanced expression of both heavy and light chains. (sigmaaldrich.com)

Using subunit-specific antibodies, however, it is not possible to distinguish between the absence of AMPA receptors and the lack of a particular subunit on the cell surface. (jneurosci.org)

Antibodies are glycoproteins belonging to the immunoglobulin superfamily . (wikipedia.org)
The terms antibody and immunoglobulin are often used interchangeably, [1] though the term 'antibody' is sometimes reserved for the secreted, soluble form, i.e. excluding B-cell receptors. (wikipedia.org)
Polyclonal immunoglobulin G (IgG) anti-Hu antibodies or type 1 antineuronal nuclear antibodies are most prevalent (~50%), although several other circulating autoantibodies have been identified. (medscape.com)

For storage sodium azide is added or you can call us to request azide free antibody preparations. (gentaur.com)
Biotium adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. (gentaur.com)

Immune thrombotic thrombocytopenic purpura (iTTP) is an acute, rare life-threatening condition associated with antibodies to ADAMTS13, resulting in severe enzyme deficiency, failure of von Willebrand factor (VWF) cleavage, excess platelet-VWF binding and microthrombi formation, resulting in multi-organ damage. (haematologica.org)
iTTP is an immune-mediated condition and antibodies to ADAMTS13 are polyclonal. (haematologica.org)
Polyclonal antibodies known as Digoxin Immune Fab are used to help people suffering from toxic poisoning brought on as a side effect from taking too much digoxin medication. (prospecbio.com)
Another polyclonal antibody, Rho(D) immune globulin, has been used to help pregnant mothers. (prospecbio.com)
Using this binding mechanism, an antibody can tag a microbe or an infected cell for attack by other parts of the immune system, or can neutralize it directly (for example, by blocking a part of a virus that is essential for its invasion). (wikipedia.org)
The constant region at the trunk of the antibody includes sites involved in interactions with other components of the immune system. (wikipedia.org)
Antibodies from different classes also differ in where they are released in the body and at what stage of an immune response. (wikipedia.org)
Together with B and T cells , antibodies comprise the most important part of the adaptive immune system . (wikipedia.org)
These data indicate that multiple antigenically distinct GII.17 variants co-circulate in young children, suggesting retention of cluster diversity alongside potential for immune escape given the existence of antibody-defined cluster-specific epitopes elicited during infection. (cdc.gov)

Detection is typically performed using peroxidase linked antibodies to catalyse a chemiluminescent reaction. (wikipedia.org)
Recombinant antibodies serve as highly specific detection probes in research, they are fast finding their niche in the detection of different pathogens and toxins and have great potential in therapeutic applications. (sigmaaldrich.com)
Description: A polyclonal antibody for detection of Cdc25C phospho Ser216) from Human. (bd-ibr.org)
Description: A polyclonal antibody for detection of Cdc25A phospho Thr507) from Human, Mouse, Rat. (bd-ibr.org)

Furthermore, spacer antibodies are more likely to inhibit ADAMT13 enzyme activity, as opposed to antibodies in the C terminal region of ADAMTS13, which result in increased ADAMTS13 clearance. (haematologica.org)

Rabbits are used for polyclonal antibody production by Biotium. (gentaur.com)
Anti-PvTRAMP antisera was obtained by inoculating rabbits with PvTRAMP B cell epitopes produced as synthetic peptides in order to assess its recognition in parasite lysates by Western blot and in intact parasites by indirect immunofluorescence. (biomedcentral.com)

However, it remains unclear how mutations that escape specific monoclonal antibodies will affect the polyclonal antibody response elicited by infection or vaccination. (biorxiv.org)
1 , 2 Despite this, and as replicated by a number of groups, nearly 100% of patients demonstrate a specific target region of antibody binding to the spacer domain in the N terminal region of the metalloprotease, ADAMTS13. (haematologica.org)
This specific exosite is critical in binding to the A2 region of VWF, but it is also the major epitope for ADAMTS13 autoantibodies. (haematologica.org)
Their specific monovalent nature makes monoclonal antibodies very useful in highly targeted immunoassays and diagnostic applications. (sigmaaldrich.com)
We have established clear experimental protocols and provide specific guidelines for working, with conformational/misfolded SOD1-specific antibodies. (nature.com)
Using these antibodies, in conjunction with GluR2-specific and synaptophysin antibodies, we have directly localized and quantified surface-expressed native AMPA and NMDA receptors on cultured living hippocampal neurons during development. (jneurosci.org)
[2] [3] Each tip of the "Y" of an antibody contains a paratope (analogous to a lock) that is specific for one particular epitope (analogous to a key) on an antigen, allowing these two structures to bind together with precision. (wikipedia.org)
The antibody was purified using epitope specific phosphopeptide. (abcam.com)
The present invention relates to an anti-tyrosinase antibody for inhibiting tyrosinase and use thereof, and more particularly, to an anti-tyrosinase antibody including a heavy chain CDR and a light chain CDR of specific sequences, or an antigen-binding fragment thereof. (justia.com)
Antibodies secreted by immortalized memory B cells (MBCs) from an infant GII.17 case were highly specific to GII.17 and exhibited blockade activity against this genotype. (cdc.gov)

Polyclonal antibodies to denatured OVA reacted strongly with both OVA forms and with several of the overlapping peptides. (ku.dk)

Because some antibodies inhibit or neutralize the activity of BoNT, the choice of antibody with which to extract the toxin is critical. (cdc.gov)

Several recent studies have identified viral mutations that impact neutralization by polyclonal human sera. (biorxiv.org)
This work has shown that single mutations to the spike's receptor-binding domain (RBD) or N-terminal domain (NTD) can appreciably reduce viral neutralization by polyclonal sera, sometimes by as much as 10-fold. (biorxiv.org)

Anatomy30

Organisms20

Diseases3

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment56

Phenomena and Processes49

Disciplines and Occupations2

Information Science4

Health Care4

Monoclonal Antibody11

Specificity6

Immunogen3

Primary Antibodies2

ELISA12

Peptide16

Reactivity3

Datasheet2

Secondary Antibodies1

Protein19

Misfolded-specific antibodies1

Heat induced epitope retrieval2

Proteins8

Linear epitopes1

Binds2

Rabbit antibodies1

React3

Serum7

Goat2

Antigenic epitopes4

Immunization3

Genes2

Sequence3

Immunity4

MAbs2

Antigens4

Given antigen2

Assays1

Catalog1

Mouse monoclonal1

Affinity-chromatography1

Amino2

Specificities1

Antigen-bindi1

Retrieval2

Phosphorylation site1

Recognize3

Elicit1

Species3

Subunit1

Immunoglobulin3

Azide2

Immune9

Detection4

Enzyme1

Rabbits2

Specific10

Peptides1

Neutralize1

Sera2