SubunitRecombinantSubunitsReactivityRabbit polyclonalDatasheetProteinsConjugatePurificationSpeciesAlexa FluorPrimary antibodyProduced against purifiedMonoclonal antibodyMRNAImmunoglobulinSpecificityEndogenous levelsDilution 1:1000BiotinSpecific antibodyKinaseCatalogHighly conservedWestern BlotImmunohistochemistryAnti-Rabbit IgGCdc21991ProductsActivityEpitopeEnzymeHeLaCellsFITCAntigenDetectionFluorescentSecondary
Subunit12
- It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. (thermofisher.com)
- CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF) , which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. (caslab.com)
- Reference: Functional expression and characterization of the Epstein-Barr virus DNA polymerase catalytic subunit. (neb.com)
- A recombinant baculovirus containing the complete sequence for the Epstein-Barr virus (EBV) DNA polymerase catalytic subunit, BALF5 gene product, under the control of the baculovirus polyhedrin promoter was constructed. (neb.com)
- The DNA polymerase and the exonuclease activities of the EBV DNA polymerase catalytic subunit were sensitive to ammonium sulfate in contrast to those of the polymerase complex purified from EBV-producing lymphoblastoid cells, which were stimulated by salt. (neb.com)
- Furthermore, the template-primer preference for the polymerase catalytic subunit was different from that for the polymerase complex. (neb.com)
- These observations strongly suggest that the presence of EBV DNA polymerase accessory protein, BMRF1 gene product, does influence the enzymatic properties of EBV DNA polymerase catalytic subunit. (neb.com)
- Description: AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly. (bioworldantibodies.com)
- DT molecule is composed of two subunits: subunit A and subunit B. The A-subunit of DT is represented by a single domain - the catalytic or C-domain which modifies eEF-2. (org.ua)
- RAB3GAP, which is involved in regulation of RAB3 activity, is a heterodimeric complex consisting a 130-kD catalytic subunit and a 150-kD noncatalytic subunit (MIM 609275). (novusbio.com)
- One or two of these subunits, together with the well-conserved catalytic subunit, can form the PP1 holoenzyme, where the regulatory subunit functions to regulate substrate specificity and/or targeting to a particular cellular compartment. (antikoerper-online.de)
- AMPK is a heterotrimer contains an alpha catalytic subunit and non-catalytic beta and gamma subunits. (prospecbio.com)
Recombinant7
- Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. (vwr.com)
- The recombinant MMP-9 catalytic domain (rcMMP-9) was expressed in Escherichia coli and exhibited high hydrolyzing activity toward gelatin. (bvsalud.org)
- Insect cells infected with the recombinant virus produced a protein of 110 kDa, recognized by anti-BALF5 protein-specific polyclonal antibody. (neb.com)
- The expressed EBV DNA polymerase catalytic polypeptide was purified from the cytosolic fraction of the recombinant virus-infected insect cells. (neb.com)
- By immunization of animals with recombinant analogues the antibodies to DT and HB-EGF were obtained. (org.ua)
- In this work, we evaluated a panel of 16 anti-BoNT/A monoclonal antibodies (mAbs) for their ability to inhibit the in vitro activity of BoNT/A1, /A2, and /A3 complex as well as the recombinant LC of A1. (cdc.gov)
- Polyclonal antibodies were raised to CYP2C9 purified from human liver, and were then back-adsorbed against recombinant CYP2C19 coupled to a solid-phase support. (aspetjournals.org)
Subunits6
- The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. (thermofisher.com)
- The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. (enzolifesciences.com)
- This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. (abbkine.com)
- Binding of insulin to the alpha subunits causes the beta subunits to phosphorylate themselves (autophosphorylation), thus activating the catalytic activity of the receptor. (sputnic-group.ru)
- Involved in regulation of the catalytic activity of the phosphatases PP2A, PP4 and PP6 by protecting their partially folded catalytic subunits from degradative polyubiquitination until they associate with regulatory subunits. (biossusa.com)
- COX, responsible for the reduction of oxygen to water, consists of three catalytic core subunits (Cox1, Cox2, and Cox3), each derived from a mitochondrial gene and of 8-10 additional structural subunits that are expressed from nuclear genes. (life-science-alliance.org)
Reactivity3
- 1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. (abbkine.com)
- No significant cross-reactivity or interference between Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) and analogues was observed. (biomatik.com)
- Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. (pp1b.com)
Rabbit polyclonal1
- Description: A Rabbit Polyclonal antibody against AFG3L2 from Human. (bioworldantibodies.com)
Datasheet1
- If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
Proteins2
- The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. (pp1b.com)
- Additionally, I describe a novel method to produce a more unbiased polyclonal generic phosphotyrosine antibody than the monoclonal antibodies that are commonly used to enrich and track tyrosine-phosphorylated proteins. (dissertation.com)
Conjugate3
- Find APOBEC3A Antibodies with a specific conjugate such as Biotin, FITC, HRP. (antibodies-online.com)
- For facs or microscopy Alexa 1 conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. (ningbobio.com)
- Anti-rabbit IgG (H+L), F(ab') 2 Fragment (PE Conjugate) #8885 was used as a secondary antibody. (cellsignal.com)
Purification3
- The enzyme consists of the catalytic domain of human MMP-1 (Phe 100 -Gln 268 ) with a C-terminal purification tag. (enzolifesciences.com)
- The enzyme consists of residues Phe 107 -Pro 449 (NM_004994), which comprises the catalytic/fibronectin domain of human MMP-9, with a C-terminal purification tag. (enzolifesciences.com)
- Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose. (kinexusproducts.ca)
Species1
- Find APOBEC3A Antibodies for a variety of species such as anti-Human APOBEC3A, anti-Mouse APOBEC3A, anti-Rat APOBEC3A. (antibodies-online.com)
Alexa Fluor1
- The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). (epigentek.com)
Primary antibody5
- Then primary antibody (1% BSA) was incubated at 4°C overnight. (epigentek.com)
- 2. According to the recommended primary antibody dilution ratio, use the TBST Buffer containing 5% Skim Milk Powder to dilute the CD38 Antibody at , soak the PVDF Membrane in the primary antibody working solution, incubate overnight at 4 ℃, and gently shake. (elabscience.com)
- The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. (cellsignal.com)
- The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. (cellsignal.com)
- After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. (cellsignal.com)
Produced against purified1
- Studies using polyclonal antibodies produced against purified mullet CYP1A also showed the similar trend. (metu.edu.tr)
Monoclonal antibody1
- The pre-coated antibody is Mouse OCT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. (biobool.com)
MRNA1
- In conclusion, feral leaping mullet caught from contaminated water displayed induction of CYP1A at three levels of expression, namely, mRNA, apoprotein and catalytic activity. (metu.edu.tr)
Immunoglobulin1
- A broad antibody response was detected that included immunoglobulin G (IgG) and IgA subtypes with demonstrable Fc-mediated effector function targeting multiple viral antigens. (cdc.gov)
Specificity2
- Unlike their biological counterparts (principally antibodies and siRNA), synthetic small molecules may be cell-permeable, easily delivered at different doses with accurate quantitation and selectable timing, and their specificity can be modified simply. (biomedcentral.com)
- This assay has high sensitivity and excellent specificity for detection of Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka). (biomatik.com)
Endogenous levels1
- COX11 Polyclonal Antibody detects endogenous levels of COX11 protein. (abbkine.com)
Dilution 1:10002
- Antibody dilution 1:1000 using ECL procedure (1 min exposure). (enzolifesciences.com)
- 1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CSNK2B-specific (20234-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. (ptglab.com)
Biotin1
- Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. (biobool.com)
Specific antibody9
- The purified protein exhibited both DNA polymerase and 3'-to-5' exonuclease activities, which were neutralized by the anti-BALF5 protein-specific antibody. (neb.com)
- This is a highly specific antibody against ADAM23. (ningbobio.com)
- B-Raf-pS729 phosphosite-specific antibody (Cat. (kinexusproducts.ca)
- B-Raf-2 pan-specific antibody (Cat. (kinexusproducts.ca)
- BRaf-3 pan-specific antibody (Cat. (kinexusproducts.ca)
- RafB-CT (RafB-4) pan-specific antibody (Cat. (kinexusproducts.ca)
- Various lysates were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. (ptglab.com)
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:200 (under 10x lens. (ptglab.com)
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:200 (under 40x lens. (ptglab.com)
Kinase4
- Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) were tested 20 times on one plate, respectively. (biomatik.com)
- Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) were tested on 3 different plates, 8 replicates in each plate. (biomatik.com)
- Just before the kinase catalytic domain. (kinexusproducts.ca)
- A single point mutation (V600E), located in the kinase catalytic domain in the activation loop between Subdomains VII and VIII. (kinexusproducts.ca)
Catalog2
- The following product was used in this experiment: CDK4 Polyclonal Antibody from Thermo Fisher Scientific, catalog # 11026-1-AP. (thermofisher.com)
- The following product was used in this experiment: EXOSC4 Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-117154, RRID AB_2901784. (thermofisher.com)
Highly conserved1
- The primary sequences of ePK catalytic domains are highly conserved, indicating a common ancestry. (dissertation.com)
Western Blot4
- Western blot analysis of extracts of Raji cells using CD38 Polyclonal Antibody at dilution of 1:1000. (elabscience.com)
- human testis tissue were subjected to SDS PAGE followed by western blot with 55388-1-AP (PKA C-alpha speicifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. (ptglab.com)
- Western Blot Antibodies - Bioworld Consulting Laboratories, LLC. (bioworldantibodies.com)
- Western Blot: RAB3GAP1 Antibody [NBP2-38206] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. (novusbio.com)
Immunohistochemistry2
- Immunohistochemistry of paraffin-embedded Human colon using CD38 Polyclonal Antibody at dilution of 1:100 (40x lens). (elabscience.com)
- Immunohistochemistry-Paraffin: RAB3GAP1 Antibody [NBP2-38206] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts. (novusbio.com)
Anti-Rabbit IgG3
- Samples were incubated with EXOSC4 Polyclonal antibody (Product # PA5-117154) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. (thermofisher.com)
- Dilute with PBS, pH 7.2-7.4 containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). (enzolifesciences.com)
- Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody. (cellsignal.com)
Cdc21
- The antibody is specific to the 2 isoforms of CDC2. (caslab.com)
19911
Products1
- Various products were used for development of diagnostic test-systems aimed at detection of antibodies to DT. (org.ua)
Activity11
- rLv-PPO showed catalytic activity towards l-Dopa and catechol. (bvsalud.org)
- Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. (thermofisher.com)
- Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. (epigentek.com)
- Metalloprotease-disintegrin MDC9: intracellular maturation and catalytic activity. (ximbio.com)
- They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. (solarbio.cc)
- To assess the importance of this carbohydrate-active enzyme in the feeding act of Orciraptor , we recombinantly produced its catalytic domain and studied the enzymatic activity, cellular localisation and function. (megazyme.com)
- Results: The GH5_5 catalytic domain from Orciraptor showed pronounced activity on soluble cellulose derivatives and mixed-linkage glucans, with reaction optima comparable to known GH5_5 representatives. (megazyme.com)
- Because some antibodies inhibit or neutralize the activity of BoNT, the choice of antibody with which to extract the toxin is critical. (cdc.gov)
- Among the mAbs, there were significant differences in extraction efficiency, ability to extract BoNT/A subtypes, and inhibitory effect on BoNT catalytic activity. (cdc.gov)
- NP antibodies are not associated with protection through neutralizing activity, but VRP vaccination results in NP antibodies possessing Fc-mediated antibody effector functions, such as complement activation (ADCD) and antibody-mediated cellular phagocytosis (ADCP). (cdc.gov)
- We used this system to evaluate the neutralization activity of monoclonal and polyclonal antibodies, plasma from NiV-infected hamsters, and serum from human patients. (cdc.gov)
Epitope1
- Find APOBEC3A Antibodies with a specific epitope. (antibodies-online.com)
Enzyme1
- The latter enzyme, called Sc LPMO10D, and most of the enzymes found in this subclade are unique, not only because of variation in the catalytic domain, but also as their C-terminus contains a cell wall sorting signal (CWSS), which flags the LPMO for covalent anchoring to the cell wall. (nature.com)
HeLa1
- Immunofluorescence staining of Hela cells with AIMP1 Polyclonal Antibody at 1:66, counter-stained with DAPI. (epigentek.com)
Cells2
- Furthermore, the anti-GH5_5 antibody applied to live cells significantly reduced the feeding success of Orciraptor . (megazyme.com)
- Confocal immunofluorescent analysis of Ramos cells, untreated (left) or IgM-treated (middle), and Jurkat cells (right) using Phospho-CD79A (Tyr182) Antibody (green). (cellsignal.com)
FITC1
- CusabioAce2 Antibody, FITC conjugated is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send. (joplink.net)
Antigen1
- productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. (pp1b.com)
Detection2
- Description: A polyclonal antibody for detection of AFG3L2 from Human. (bioworldantibodies.com)
- Run a rapid chromatographic immunoassay for the qualitative detection of LH in urine to aid in the detection of ovulation, containing the anti-LH particles and anti-LH antibody coated on the membrane. (itmedicalteam.pl)
Fluorescent1
- Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes. (kinexusproducts.ca)
Secondary1
- The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. (epigentek.com)