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Chromatography, High Pressure LiquidChromatography, AffinityElectrophoresis, Polyacrylamide GelChromatography, GelChromatographyGelsChromatography, Ion ExchangeChromatography, GasMolecular WeightChromatography, LiquidChromatography, Thin LayerChromatography, DEAE-CelluloseKineticsMolecular Sequence DataAmino AcidsChromatography, AgaroseIsoelectric FocusingAmino Acid SequenceMass SpectrometryElectrophoresis, Gel, Two-DimensionalHydrogen-Ion ConcentrationSubstrate SpecificityGas Chromatography-Mass SpectrometryChromatography, Reverse-PhaseChemistryChemical PhenomenaSepharoseIsoelectric PointChromatography, PaperCattleImmunodiffusionEscherichia coliCarbohydratesElectrophoresis, DiscRabbitsMacromolecular SubstancesSpectrophotometry, UltravioletAcrylic ResinsBase SequencePeptide FragmentsTrypsinLiverMethodsElectrophoresisTemperatureTandem Mass SpectrometryGlycoproteinsChemical FractionationPeptidesElectrophoresis, Agar GelCentrifugation, Density GradientProtein BindingDrug StabilityImmunoelectrophoresisChemical PrecipitationHydroxyapatitesBacterial ProteinsHydrolysisSodium Dodecyl SulfateSpectrometry, Mass, Electrospray IonizationSpecies SpecificitySolubilityCloning, MolecularRecombinant ProteinsIndicators and ReagentsCarbohydrate SequenceHot TemperatureOligosaccharidesCell LineProteinsAmmonium SulfateCountercurrent DistributionIsoenzymesChromatography, Micellar Electrokinetic CapillaryBinding SitesUltracentrifugationMagnetic Resonance SpectroscopySwineRadioimmunoassayDNACell MembraneImmunochemistryTritiumLectinsRats, Inbred StrainsSpectrophotometryImmune SeraElectrophoresis, Gel, Pulsed-FieldDetergentsCarrier ProteinsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationBiological AssayPolysaccharidesPeptide HydrolasesAcrylamidesBlood ProteinsReproducibility of ResultsMicrochemistryMembrane ProteinsCross Reactions
High performance liSize exclusion chroExchange ChromatographyHPLCElectrophoresisProteinsPurificationSilica gelCharacterizationMonomerFractionsMass spectrometryPeptideAnalyticalFiltrationAffinityProteasesEnzymeMolecularTemperatureAcrylamideCompoundsLiquidProcedureMethodBufferAnalysisMethodsStudyMatrixSeparateContentProduceProvideActivationAdditionActivityRoleAgentsNaturalSolutionFree
High performance li6
Size exclusion chro4
  • Two original products, Eprex (epoetin alfa) and Dynepo (epoetin delta), and two biosimilar products, Binocrit (epoetin alfa) and Retacrit (epoetin zeta), were compared using (1) high performance size exclusion chromatography, (2) ELISA, (3) SDS-PAGE, (4) capillary zone electrophoresis and (5) in-vivo potency. (springer.com)
  • Because a limited amount of EPO products was available, we were restricted to the common assays used for quality control: high performance size exclusion chromatography (HP-SEC) to assess monomer and aggregate content, ELISA to determine EPO protein content, SDS-PAGE under non-reducing conditions to detect possible fragmentation and covalent protein aggregates, capillary zone electrophoresis (CZE) for isoform profiling and a normocythemic mouse assay to test for potency. (springer.com)
  • A comparative study of AF4 with size exclusion chromatography (SE-HPLC) unveiled SE-HPLC to inhere higher recovery rates and AF4 to exhibit greater resolution. (uni-muenchen.de)
  • Sera of rabbits with hypoxia were fractionated by size exclusion chromatography, the fractions were tested for their ability to modify the expression of P450 isoforms, and serum mediators were identified through neutralization experiments. (aspetjournals.org)
Exchange Chromatography5
  • The present study aims to characterize the Cry2Ad toxin protein isolated from a Bacillus thuringiensis strain, BRC-HZP10, which have a potential insecticidal activity against larvae of the diamondback moth, Plutella xylostella (L.). The crude Bt toxin proteins were isolated and purified by cation exchange chromatography, then equilibrated with 0.2 M NaOH buffer, pH 4.0, followed by ultraviolet detection at 280 nm and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. (geneticsmr.com)
  • RPC & RP-HPLC HPLC, RP-HPLC and RPC all employ a similar underlying principle, which was first used in Ion Exchange Chromatography. (genelink.com)
  • Based on the properties of the protein to be purified, appropriate methods are selected such as ion exchange chromatography, gel filtration chromatography, and affinity chromatography. (fujifilm.com)
  • In the first dimension, ion-exchange chromatography (IEC) separates peptides based on ionic interactions with the solid phase and an increasing salt buffer. (hindawi.com)
  • XPB-6 was purified to homogeneity with 2.15 fold of purity and 60.31% yield, respectively by ammonium sulfate precipitation and DEAE ion exchange chromatography. (scirp.org)
HPLC4
  • Oligo purification Gene Link provides polyacrylamide gel electrophoresis purification (PAGE) and Reverse Phase Cartridge (RPC) (as a substitute for Reverse Phase HPLC (RP-HPLC). (genelink.com)
  • Gel purification routinely yields 99% pure full-length product and RPC and RP-HPLC purification yields a purity of 85%-96% depending on the oligo length, base composition and sequence. (genelink.com)
  • RPC purification employs the same principle as that of Reverse Phase High Pressure Liquid chromatography (RP-HPLC). (genelink.com)
  • Also, prep-HPLC is an effective technology currently used for natural product isolation in industry ( 17 ). (spandidos-publications.com)
Electrophoresis10
  • SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins with molecular masses between 5 and 250 kDa. (wikipedia.org)
  • Alternatively, polyacrylamide gel electrophoresis can also be performed with the cationic surfactants CTAB in a CTAB-PAGE, or 16-BAC in a BAC-PAGE. (wikipedia.org)
  • The SDS-PAGE method is composed of gel preparation, sample preparation, electrophoresis, protein staining or western blotting and analysis of the generated banding pattern. (wikipedia.org)
  • When using different buffers in the gel (discontinuous gel electrophoresis), the gels are made up to one day prior to electrophoresis, so that the diffusion does not lead to a mixing of the buffers. (wikipedia.org)
  • Protein electrophoresis is an effective method to separate specific proteins from a protein mixture. (fujifilm.com)
  • The in-gel digestion methodology has become routine for proteins separated by 1-D or 2-D electrophoresis. (creative-proteomics.com)
  • Proteins of pooled ticks were separated by 1-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and gel slices were digested in situ with trypsin before using LC-MS/MS. Upwards of ≈7,000 spectra were submitted to a protein identification algorithm for each pooled sample. (cdc.gov)
  • The Anomalous Behavior of Collagen Peptides on Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis is human to the Low Content of Hydrophobic Amino Acid Residues. (naturheilpraxis-gisbert-fussek.de)
  • Electrophoresis is often a companion technique to chromatography because it allows researchers an orthogonal method with which to visualize the protein species present in a sample. (bioradiations.com)
  • Native gel electrophoresis revealed a rapidly-modulated reciprocal relationship between the burden of unfolded proteins and BiP oligomers and slower equilibration between oligomers and inactive, covalently-modified BiP. (elifesciences.org)
Proteins8
  • The combined use of sodium dodecyl sulfate (SDS, also known as sodium lauryl sulfate) and polyacrylamide gel eliminates the influence of structure and charge, and proteins are separated by differences in their size. (wikipedia.org)
  • The intrinsic charges of the proteins are negligible in comparison to the SDS loading, and the positive charges are also greatly reduced in the basic pH range of a separating gel. (wikipedia.org)
  • Two of the phosphotyrosine-containing proteins, migrating in SDS-polyacrylamide gels of approximately 43 and 45 kDa, corresponded in mobility to phosphorylated species of the 42- and 44-kDa mitogen-activated protein kinase (MAPK) isoforms. (nih.gov)
  • In SDS-PAGE, proteins are given negative charges by addition of sodium dodecyl sulfate (SDS) and then electrophoresed on a polyacrylamide gel. (fujifilm.com)
  • Summary, conclusions and prospective 177 Given the background that effective AF4 fractionations are due to differences in analyte size - i.e., in diffusion coefficients -, the separation of equal-sized proteins is prima facie considered to be impractical. (uni-muenchen.de)
  • Yet, the retaining impact of sample-membrane interaction was evidenced to decrease the effective diffusion coefficient, resulting in successful fractionations of proteins differing ~1% in size (i.e. (uni-muenchen.de)
  • Though many methods are used in the process of obtaining pure proteins, chromatography is commonly used to separate complex biological mixtures and isolate proteins of interest prior to downstream characterization. (bioradiations.com)
  • One of the most widely used methods for the isolation and purification of recombinant proteins is chromatography. (bioradiations.com)
Purification10
  • Gel purification is the gold standard of oligo purification methods and routinely yields ~99% full length product and is not dependent on size, base composition or sequence. (genelink.com)
  • Both methods provide nearly identical purification and are effective up to a length of 40mer. (genelink.com)
  • For oligos over 40mer or shorter oligos to be used in cloning or other precision downstream applications, we recommend gel purification. (genelink.com)
  • Gel purification separates exclusively by charge, which is equivalent to size in linearized DNA. (genelink.com)
  • Column chromatography is often used for protein purification. (fujifilm.com)
  • various drawbacks have been identified in the purification techniques of ion resin adsorption ( 12 , 13 ), silica gel column chromatography ( 14 ) and supercritical extraction ( 15 ). (spandidos-publications.com)
  • Nowadays, automating chromatography is helping to save time and effort in protein purification. (bioradiations.com)
  • This chromatogram and SDS-PAGE gel highlight the importance of protein purity assessment throughout your purification workflow. (bioradiations.com)
  • Over the past three decades, chromatography, specifically liquid chromatography, has become the technology of choice in the purification of biotherapeutics, due in large part to its resolving power. (bioradiations.com)
  • Bio-Rad's NGC™ Chromatography Systems were designed with this is mind and provide a range of customizable chromatography systems that can increase purification capabilities and be adapted to meet growing throughput needs, obviating the need to buy a new instrument. (bioradiations.com)
Silica gel3
  • This is based on thin-layer chromatography (TLC) performed on silica gel [ 13 ] . (encyclopedia.pub)
  • Active compounds are identified on the basis of their Rf values, while nonidentifiable active volatile compounds can be cut out from the silica gel and analyzed using the headspace solid-phase microextraction method coupled to gas chromatography-mass spectrometry (HS-SPME/GC-MS) [ 14 ] . (encyclopedia.pub)
  • With this, the purity or percentage composition of antimicrobial active compounds from silica gel can be determined. (encyclopedia.pub)
Characterization1
  • We conclude that this analytical platform is effective for the characterization of complex bioactive samples. (hindawi.com)
Monomer1
  • The residual monomer content of polyacrylamide produced by this method can be as low as 0.02% ( Gas chromatography). (gdsxhb.com)
Fractions1
  • Chromatography of soluble fractions from these cells on Mono Q columns revealed early and late eluting peaks of myelin basic protein kinase activity, which contained the 42- and 44-kDa MAPK isoforms, respectively. (nih.gov)
Mass spectrometry3
  • The peptide fragments are then recovered from the gel and subjected to mass spectrometry (LC-MS/MS or MALDI-TOF MS) to determine the mass of each fragment. (fujifilm.com)
  • The whole cortex of wild-type and symptomatic RTT male littermates ( n = 4 per genotype) were analyzed using RNA-sequencing and data-independent acquisition liquid chromatography tandem mass spectrometry. (biomedcentral.com)
  • A proteomics informed by transcriptomics (PIT) approach was applied to analyse samples of female and male saliva separately using the previously obtained O. erraticus sialotranscriptome as a reference database and two different mass spectrometry techniques, namely liquid chromatography-tandem mass spectrometry (LC-MS/MS) in data-dependent acquisition mode and sequential window acquisition of all theoretical fragment ion spectra MS (SWATH-MS). (biomedcentral.com)
Peptide1
Analytical1
Filtration1
Affinity5
  • The vitellogenin was partially purified and isolated by low-ionic-strength precipitation and DEAE-affinity chromatography. (lsu.edu)
  • If the protein of interest has no ligand that can be used for affinity chromatography, an affinity tag (epitope tag) can be added to the protein and purified using the ligand for the affinity tag. (fujifilm.com)
  • In this study, we reveal the suppression of non-specific hydrophobic interaction in poly(ethylene-co-glycidylmethacrylate) (PEGM) based spongy monolith (SPM), PEGM-SPM, which has recently be reported as a new platform of separation medium for affinity chromatography in our previous study, by an simple acidic treatment. (go.jp)
  • Additionally, the immobilization procedures of protein-A toward the PEGM-SPM and the separation conditions for immunoglobulin G (IgG) were optimized for further effective affinity separations. (go.jp)
  • Various forms of chromatography, including affinity, ion exchange, and mixed mode, are now being utilized to ensure that the final product is as pure as can be without compromising yield. (bioradiations.com)
Proteases1
  • A piece of gel containing the target protein is excised, and the protein is digested in the gel by proteases (peptidases). (fujifilm.com)
Enzyme2
  • The enzyme requires divalent cations in the activity assay, Mg2+ being most effective. (uni-regensburg.de)
  • Use of abundantly available agro-residues in fermentation processes serves the dual purpose of cost effective enzyme production and environment security [9]. (scirp.org)
Molecular2
  • Therefore, gamma irradiation was an effective method for producing high-molecular-weight sericin and for developing functional foods and cosmetics. (interleukinreceptor.com)
  • At least two subunits having molecular weights of 112,000 and 40,000 daltons, respectively, were identified by calibrated SDS-polyacrylamide gels. (lsu.edu)
Temperature1
  • Since the monomers are not volatile and cannot be removed after the reaction, unreacted monomers will remain in the polyacrylamide, prolonging the reaction time, and increasing the reaction temperature can reduce the amount of residual monomers, but the production capacity decreases and the insoluble content will increase. (gdsxhb.com)
Acrylamide1
  • For the gel solution, acrylamide is mixed as gel-former (usually 4% V/V in the stacking gel and 10-12 % in the separating gel), methylenebisacrylamide as a cross-linker, stacking or separating gel buffer, water and SDS. (wikipedia.org)
Compounds1
  • Maca polysaccharides are one of the main effective components of Maca and can be explored as sources of bioactive compounds [ 13 ]. (hindawi.com)
Liquid1
  • Spherical polyimide (PI) particles have been introduced as a stationary phase in liquid chromatography (LC). (go.jp)
Procedure1
  • One key conclusion is the importance of excess amounts of collagen degradation activity in the islet isolation procedure to ensure effective degradation of native collagen. (lidsen.com)
Method3
  • The most effective for activity screening is the simple drop plate or paper filter-based disc diffusion method [ 1 ] , which is used on the lawn of the test organism. (encyclopedia.pub)
  • Method for industrial production of polyacrylamide at home and abroad-Guangdong Shouxin Environmental Protection Materials Technology Co., Ltd. (gdsxhb.com)
  • The polyacrylamide synthesized according to the above method is a high-viscosity fluid or a gel-like non-flowing substance. (gdsxhb.com)
Buffer1
  • The lower gel (separating gel) is poured first and covered with a few drops of a barely water-soluble alcohol (usually buffer-saturated butanol or isopropanol), which eliminates bubbles from the meniscus and protects the gel solution of the radical scavenger oxygen. (wikipedia.org)
Analysis3
Methods1
  • At present, domestic and foreign industrial production methods of polyacrylamide are as follows. (gdsxhb.com)
Study1
  • It is very urgent to study the pathogenesis of metabolic diseases and take effective treatment measures to delay the progress of metabolic diseases. (ijpsonline.com)
Matrix1
  • The medium (also referred to as ′matrix′) is a polyacrylamide-based discontinuous gel. (wikipedia.org)
Separate1
Content1
  • 3.1 When the provider accepts the transfer or the customer's order, an agreement is concluded between the provider and the customer, the scope and content of which is effective from the date of acceptance. (erlegr.click)
Produce1
  • In order to produce polyacrylamides containing the fewest hair groups, an appropriate amount of sodium carbonate can be added to the polymerization formula to hydrolyze a small amount of amide groups into carboxyl groups, reducing the formation of insolubles. (gdsxhb.com)
Provide1
Activation1
  • In this project, Bio Normalizer will be tested to see if it is effective in inhibiting NF-ƙB and HIV activation. (osri.asia)
Addition1
Activity1
  • TAK-285 was as effective as lapatinib in antitumor activity in a mouse subcutaneous BT-474 breast cancer xenograft model. (jcancer.org)
Role2
  • These results indicate that the Cry2Ad protein plays an effective role in controlling the population of P. xylostella . (geneticsmr.com)
  • This work revealed the important role of proline as an effective compatible solute that is required to protect cells against butanol chaotropic effect and to maintain cellular functions in B. subtilis 168 during butanol exposure. (biomedcentral.com)
Agents1
  • Consequently, there remains an urgent need to develop new effective agents for these patients ( 2 , 3 ). (jcancer.org)
Natural1
  • Natural resilin, the rubber-like protein that exists in specialized compartments of most arthropods, possesses excellent mechanical properties such as low stiffness, high resilience and effective energy storage. (unh.edu)
Solution2
  • For pouring the gel solution, the plates are usually clamped in a stand which temporarily seals the otherwise open underside of the glass plates with the two spacers. (wikipedia.org)
  • 2. In-solution or in-gel digestion. (creative-proteomics.com)
Free2
  • The gel is produced by free radical polymerization in a mold consisting of two sealed glass plates with spacers between the glass plates. (wikipedia.org)
  • Scientists can characterize drug targets and potential therapeutics with greater confidence when using stain-free gels and gel imaging instruments. (bioradiations.com)