Podocyte and glomerular. Medical search. Frequent questions

Image is low magnification vies of the basement membrane filtration slits, urinary space, and the glomerular filtration capillary lumen. (cellimagelibrary.org)
The series of images in this group have particular historic and cell biologic importance for determining the role of the glomerular basement membrane in urinary filtration, especially the charge properties and size selectivity in retention of proteins in the circulation. (cellimagelibrary.org)
Podocytes secrete and maintain the basement membrane. (wikipedia.org)
We propose the outline of such a mechanism based on following aspects: (1) The glomerulus is a complex structure, the stability of which depends on the cooperative function of the basement membrane, mesangial cells and podocytes, counteracting the distending forces originating from the high glomerular hydrostatic pressures. (scienceopen.com)
No specific or efficient treatment exists for Alport syndrome, an X-linked hereditary disease caused by mutations in collagen type IV, a crucial component of the glomerular basement membrane. (hindawi.com)
Microhematuria and subsequently proteinuria are hallmarks of kidney involvement, which are due to primary basement membrane alterations that mainly cause endothelial thrombosis and podocyte contraction and ulterior irreversible detachment. (hindawi.com)
What might your study reveal about thickening of the glomerular basement membrane-the cause of membranous nephropathy? (renalandurologynews.com)
It has long been recognized that the thickening of the glomerular basement membrane (GBM) is a reaction to the injury of presumed antigen-antibody complexes depositing on the subepithelial surface of the GBM. (renalandurologynews.com)
The podocyte is a highly differentiated cell located in the outer space of the glomerular basement membrane that deals with many different functions. (degruyter.com)
podocytes run the risk of detachment from the basement membrane due to a decrease in the adherence to the surrounding matrix after contraction, a process called foot processes effacement. (degruyter.com)
Podocytes change their shape and under constant mechanical stress they finally detach, rendering the glomerular basement membrane unprotected unless other contiguous podocytes are capable of covering the surface. (degruyter.com)
The glomerular filtration barrier mainly exerts its function via three layers: fenestrated endothelium, glomerular basement membrane (GBM), and podocytes. (biomedcentral.com)
We have recently begun studying how transitions in the repertoire of integrin adhesion receptors for the glomerular basement membrane during podocyte injury affect the transcriptional response. (bidmc.org)
Membranous nephropathy is an autoimmune disorder in which immune complexes deposit along the subepithelial region of the glomerular basement membrane. (medscape.com)
Membranous nephropathy is deposition of immune complexes on the glomerular basement membrane (GBM) with GBM thickening. (msdmanuals.com)

Human renal glomerular endothelial cells (HRGEnC) (ScienCell) were maintained in endothelial cell medium ECM (ScienCell). (biomedcentral.com)

The Kreidberg-Schumacher laboratory's research is focused on the molecular biology of glomerular diseases such as Focal Segmental Glomerular Sclerosis (FSGS), with the goal of developing novel therapeutic approaches to prevent End Stage Kidney Disease. (bidmc.org)
The laboratory studies how transcriptional regulation by WT1 and other transcription factors regulates the podocyte response to injury, thus determining whether injury may be repaired or leads to irreversible kidney diseases such as focal segmental glomerular sclerosis (FSGS). (bidmc.org)
Faulty podocyte structure and filtration also characterize diabetic nephropathy and glomerular diseases (e.g., focal and segmental glomerular sclerosis), which were once considered orphan diseases but have become more prevalent in recent years as sedentary lifestyles and diets high in processed foods have become commonplace. (sciencedaily.com)
Today we'll be discussing focal segmental glomerular sclerosis (FSGS) with my guest, Dr Kirk Campbell. (medscape.com)

The images here clearly show the glomerular filtration barrier. (cellimagelibrary.org)
Nephrotis syndrome occurs when there is a breakdown of the glomerular filtration barrier. (wikipedia.org)
The podocytes form one layer of the filtration barrier. (wikipedia.org)
Genetic mutations can cause podocyte dysfunction leading to an inability of the filtration barrier to restrict urinary protein loss. (wikipedia.org)
We found ten different NPHS2 mutations, comprising nonsense, frameshift and missense mutations, to segregate with the disease, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier. (scienceopen.com)
Polarity signaling through the atypical PKC (aPKC)-Par polarity complex is essential for the development and maintenance of the podocyte architecture and the function of the glomerular filtration barrier of the kidney. (uni-koeln.de)
Despite its well-established role in aPKC-mediated signaling, Par3A appears to be dispensable for the function of the glomerular filtration barrier. (uni-koeln.de)
In conclusion, Par3A function is either dispensable for slit diaphragm integrity, or compensatory mechanisms and a high redundancy of the different polarity proteins, including Par3B, Lgl, or PALS1, maintain the function of the glomerular filtration barrier, even in the absence of Par3A. (uni-koeln.de)
Podocytes are the last barrier of the glomerular filtration membrane, and podocyte dysfunction can cause proteinuria and glomerular sclerosis, exacerbating the progression of DN [ 3 ]. (hindawi.com)
We are investigating how podocyte injury results in transcriptional reprogramming that determines whether podocytes are able to repair the glomerular filtration barrier, or suffer irreversible damage, leading to proteinuria and End Stage Kidney Disease. (bidmc.org)
Neph1 is essential for the maintenance of podocytes--neuron-like cells with long finger-like projections that wrap around the glomerular capillaries, serving as the final barrier between the blood and the urine. (sciencedaily.com)
In addition, treatment with Nutlin-3a caused in healthy mice stabilization of p53 levels and subsequent podocytopenia, promoting albuminuria and impaired glomerular filtration barrier. (uni-muenchen.de)
Schematic drawing of the glomerular barrier. (medscape.com)

In 2002 Professor Moin Saleem at the University of Bristol made the first conditionally immortalised human podocyte cell line. (wikipedia.org)
The human podocyte cell line was derived from human normal podocytes conditionally transformed with a temperature-sensitive mutant of the simian virus 40 (SV40) large T antigen. (biomedcentral.com)

Podocytes have primary processes called trabeculae, which wrap around the glomerular capillaries. (wikipedia.org)

Its cationic nature allows binding to the anionic glomerular capillary wall with resultant immune complex formation, a parallel to experimental models. (medscape.com)

Podocyte-specific overexpression of human angiotensin-converting enzyme 2 attenuates diabetic nephropathy in mice. (ohri.ca)
Protection of CTGF Antibody Against Diabetic Nephropathy in Mice Via Reducing Glomerular β-Catenin Expression and Podocyte Epithelial-Mesenchymal Transition. (ncbcs.org)
mTORC1 activation inhibits autophagy, and it is considered to be a molecular marker of diabetic nephropathy [ 7 , 8 ], as the decrease in podocyte autophagic activity caused by mTORC1 activation plays a vital role in the progression of this disease [ 9 ]. (hindawi.com)
More importantly, astragaloside IV (the main component of Astragali Radix) and emodin (the main component of Rhei Radix Et Rhizoma) ameliorate podocyte damage in diabetic nephropathy by enhancing podocyte autophagy [ 13 , 16 ]. (hindawi.com)
Their findings suggest that Myo1c uses the actin cytoskeleton as a 'track' for Neph1 transport -- a finding with translational relevance for glomerular diseases such as diabetic nephropathy, as movement of Neph1 to and from the surface membrane triggers the injury/recovery response. (sciencedaily.com)

It has subsequently been found to be located in the glomerular epithelial cell, i.e., the podocyte. (renalandurologynews.com)

Podocytes possess a well-developed endoplasmic reticulum and a large Golgi apparatus, indicative of a high capacity for protein synthesis and post-translational modifications. (wikipedia.org)
Disruption of the filtration slits or destruction of the podocytes can lead to massive proteinuria, where large amounts of protein are lost from the blood. (wikipedia.org)
NPHS2, encoding the glomerular protein podocin, is mutated in autosomal recessive steroid-resistant nephrotic syndrome. (scienceopen.com)
NPHS2 is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. (scienceopen.com)
Glomerular epithelium cell (podocyte) (at protein level). (joplink.net)
The impaired motility depends on viral replication and transfection of podocytes with N protein of PUUV or HTNV reveals that the expression of N protein alone is sufficient to deteriorate podocyte function. (biomedcentral.com)
Hantaviral N protein was detected in tubular and glomerular cells and cell-to-cell contact structures were changed in biopsy samples of patients with serologically confirmed PUUV infections [ 3 ]. (biomedcentral.com)
A flexible, multilayered protein scaffold maintains the slit in between glomerular podocytes. (uni-koeln.de)
When podocyte structure fails, the kidney is no longer able to act as an effective filter, and excessive protein escapes the capillaries into the urine. (sciencedaily.com)
This heavily glycosylated mucin type protein is named for its expression on kidney glomerular epithelial cells known as podocytes. (bdbiosciences.com)
3 g of protein/day due to a glomerular disorder plus edema and hypoalbuminemia. (msdmanuals.com)

CD2AP regulates the podocyte cytoskeleton and stabilizes the slit diaphragm. (wikipedia.org)

These findings broaden our understanding of the pathogenesis of 2 different glomerular diseases: RPGN and FSGS. (scienceopen.com)
Progression of glomerular diseases: is the podocyte the culprit? (scienceopen.com)
Urinary podocyte loss is a silent phenomenon that precedes proteinuria in glomerular diseases [ 3 , 4 ]. (hindawi.com)
The Beck / Salant lab explores the immune basis of glomerular diseases and the mechanisms of podocyte injury. (bu.edu)
Therefore, in diseases that can threaten the glomerular normal environment, the presence and the quantification of urinary podocytes can be of remarkable relevance, as it can herald or accompany the appearance of proteinuria, and could offer another view to the interpretation and clinical approach and outcome of proteinuria. (degruyter.com)
Understanding whether that transport contributes to protection or injury could have translational importance for the treatment of glomerular diseases," says Nihalani. (sciencedaily.com)

Beforehand, we've got demonstrated its protecting function in glomerular mesangial cells. (holliseden.com)

To mimic activation of Gα12 pathways, constitutively active Gα12 (QL) was conditionally expressed in podocytes using Nphs2-Cre and LacZ/floxed QLα12 transgenic mice. (medscape.com)

Although various viscera have epithelial layers, the name visceral epithelial cells usually refers specifically to podocytes, which are specialized epithelial cells that reside in the visceral layer of the capsule. (wikipedia.org)

DN progression is associated with podocyte damage due to reduced autophagy caused by mTORC1 activation. (hindawi.com)

The glomerular podocyte is particularly sensitive to mitochondrial dysfunction, as seen in various genetic disorders manifesting as progressive glomerulosclerosis. (jci.org)
Podocyte-specific deletion of Klf6 expression in mice leads to mitochondrial dysfunction and apoptosis, followed by glomerulosclerosis. (jci.org)

The podocytes have long foot processes called pedicels, for which the cells are named (podo- + -cyte). (wikipedia.org)
The foot processes known as pedicels that extend from the podocytes wrap themselves around the capillaries of the glomerulus to form the filtration slits. (wikipedia.org)
A loss of the foot processes of the podocytes (i.e., podocyte effacement) is a hallmark of minimal change disease, which has therefore sometimes been called foot process disease. (wikipedia.org)
Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. (joplink.net)

Podocyte Injury and Albuminuria in Mice with Podocyte-Specific Overexpression of the Ste20-Like Kinase, SLK. (ohri.ca)

Chronic kidney disease (CKD) is a chronic complication of diabetes, characterized by the presence of pathological quantities of urine albumin excretion and/or accompanied by a gradual deterioration in the glomerular filtration rate (GFR). (medsci.org)
Podocytes work with the endothelium of the capillaries to form a thin filter to separate urine from blood passing through the glomerulus. (innerbody.com)
The outer layer of the glomerular capsule holds the urine separated from the blood within the capsule. (innerbody.com)
The renal tubule carries urine from the glomerular capsule to the renal pelvis. (innerbody.com)
Noteworthy, the podocytes that are encountered in the urine are viable despite the glomerular hostile environment and the urinary acidity. (degruyter.com)

We performed whole animal studies and used biosensor measurements, electron microscopy, electrophysiology, and live calcium imaging experiments to evaluate the contribution of this pathway to the physiology of the podocytes in freshly isolated glomeruli.Results Upon induction of type 1 diabetes with streptozotocin, SSNox4-/- rats exhibited significantly lower basal intracellular Ca2+ levels in podocytes and less DKD-associated damage than SS rats did. (siu.edu.ar)
Finally, electron microscopy revealed that podocytes of glomeruli isolated from Trpc6-knockout or Trpc5/6 double-knockout mice were protected from damage induced by H2O2 to the same extent.Conclusions These data reveal a novel signaling mechanism involving NOX4 and TRPC6 in podocytes that could be pharmacologically targeted to abate the development of DKD. (siu.edu.ar)

Podocytes are cells in Bowman's capsule in the kidneys that wrap around capillaries of the glomerulus. (wikipedia.org)
Responsible for filtering the blood , our renal corpuscle is formed by the capillaries of the glomerulus and the glomerular capsule (also known as Bowman's capsule). (innerbody.com)
Special epithelial cells known as podocytes form the layer of the glomerular capsule surrounding the capillaries of the glomerulus. (innerbody.com)

Abstract: Background: Loss of glomerular podocytes is an indicator of diabetic kidney disease (DKD). (siu.edu.ar)

You've become a leader, and this is an area that has really been expanding over the past decade - FSGS and glomerular disorders in general. (medscape.com)

Ubiquitin C-terminal hydrolase L1 deletion ameliorates glomerular injury in mice with ACTN4-associated focal segmental glomerulosclerosis. (ohri.ca)
We hypothesized that podocytes are susceptible to filtered plasma components, including hormones and growth factors that stimulate signaling pathways leading to glomerulosclerosis. (medscape.com)
Herein, we report that genetic activation of Gα12 in podocytes leads to time-dependent increases in proteinuria and glomerulosclerosis. (medscape.com)

Urinary Podocyte Microparticles Identify Prealbuminuric Diabetic Glomerular Injury. (ohri.ca)
Podocyte injury associated with mutant alpha-actinin-4. (ohri.ca)
Accumulation of α-synuclein mediates podocyte injury in Fabry nephropathy. (uib.no)
Herein, in vitro experiments using a high glucose-induced podocyte injury model were performed. (hindawi.com)
Experimental research found a markedly decreased expression of autophagy-related proteins such as Beclin-1, Atg12-Atg5, and LC3-II in a diabetic mouse model and in a high glucose-induced podocyte injury model [ 4 ]. (hindawi.com)
We are studying the function of transcription factors crucial to maintaining gene expression in podocytes and that modulate a repair response after injury. (bidmc.org)
The laboratory's research interest is focused the molecular biology of glomerular disease, and particularly on injury to podocytes, a key cell type in the glomerulus. (bidmc.org)
Most recently, he has combined his interest in cell adhesion and transcriptional regulation, through studies on how integrins regulate the YAP/TAZ transcription factors during podocyte injury. (bidmc.org)
To solve that mystery, Nihalani and his collaborators have developed a mouse model in which Myo1c is knocked out in the podocytes and are conducting experiments to better understand the effects of Myo1c knockout on the injury/recovery response. (sciencedaily.com)
Taken together, these studies suggest that Gα12 activation promotes glomerular injury without podocyte depletion through a novel mechanism regulating collagen (α)IV expression, and supports the notion that glomerular damage may accrue through persistent GPCR activation in podocytes. (medscape.com)
Gα12/13 are expressed in podocytes and couple to angiotensin II, thrombin, endothelin and LPA receptors, which are important in renal injury. (medscape.com)

Using these methods, it could be established that the proliferating cells in Bowman's space, which are characteristically found in RPGN, are derived almost exclusively from the glomerular epithelium - primarily PECs. (scienceopen.com)
Surrounding the glomerulus is the glomerular capsule, a cup-shaped double layer of simple squamous epithelium with a hollow space between the layers. (innerbody.com)

The renal manifestations of SCD range from various tubular and glomerular functional abnormalities to gross anatomic alterations of the kidneys. (medscape.com)
Renal filtration depends on the integrity of epithelial and endothelial monolayers in the tubular and glomerular apparatus. (biomedcentral.com)
Tubular and glomerular cells represent target cells of hantavirus infection. (biomedcentral.com)
Normal kidney filter function depends on the integrity of GBM and of tubular and glomerular monolayers. (biomedcentral.com)
Its administration in a mouse AKI model showed a diminished capacity of healing and the specific deletion of Mdm2 in both podocytes and tubular cells induced p53-overexpression dependent cell death (podoptosis). (uni-muenchen.de)
For this reason we hypothesized that the administration of Nutlin-3a to healthy mice damages renal function by promoting podoptosis in podocytes and tubular cells. (uni-muenchen.de)
Consistent with the hypothesis, we observed that stimulation with Nutlin-3a increased cell death in cultured mouse podocytes and tubular cells. (uni-muenchen.de)

The present case illustrates the reduction rate of urinary podocyte loss and proteinuria after amiloride administration and suggests the molecular pathways involved in Alport renal disease. (hindawi.com)

We hypothesized that NOX4-derived H2O2 contributes to podocyte damage in DKD via elevation of podocyte calcium.Methods We used Dahl salt-sensitive (SS) rats with a null mutation for the Nox4 gene (SSNox4-/-) and mice with knockout of the nonselective calcium channel TRPC6 or double knockout of TRPC5 and TRPC6. (siu.edu.ar)
H2O2 stimulated TRPC-dependent calcium influx in podocytes from wild-type mice, but this influx was blunted in podocytes from Trpc6-knockout mice and, in a similar manner, in podocytes from Trpc5/6 double-knockout mice. (siu.edu.ar)

Nephropathy and Elevated BP in Mice with Podocyte-Specific NADPH Oxidase 5 Expression. (ohri.ca)
Expression of SHP-1 induced by hyperglycemia prevents insulin actions in podocytes. (ohri.ca)
In this issue of the JCI , Mallipattu and coworkers show that KLF6 expression is reduced in mouse and human glomerular disease. (jci.org)
Results showed that TSN treatment enhanced the weakened nephrin expression and autophagic activity of podocytes and inhibited the mTORC1 pathway (p-mTOR, mTOR, p-p70S6K, p70S6K, ULK1, and 4EBP1) under high glucose conditions. (hindawi.com)

QLα12 LacZ+/Cre+ mice showed no changes in podocyte number, apoptosis, proliferation or Rho/Src activation. (medscape.com)

Neutral endopeptidase, a podocyte antigen that can digest biologically active peptides, was identified as the target antigen in a subset of patients with antenatal membranous nephropathy. (medscape.com)
The M-type phospholipase A2 receptor (PLA2R) in the glomerular podocyte has been identified as the major target antigen in deposited immune-complexes. (msdmanuals.com)

When podocytes contract, they cause closure of filtration slits. (wikipedia.org)

The stereotyped development of the glomerular lesions in many animal models and human forms of progressive renal disease suggests that there are common mechanisms of disease progression. (scienceopen.com)
Podocyte damage caused by weak autophagic activity is an essential mechanism underlying DN progression [ 5 ]. (hindawi.com)

In summary, TSN reduces podocyte damage induced by high glucose via inhibiting mTORC1 pathway and downstream targets and restoring podocyte autophagy. (hindawi.com)
TSN has also been confirmed to reduce podocyte damage in DN rats and high glucose-cultured podocytes [ 11 , 12 ]. (hindawi.com)
We have now determined that in cultured podocytes, high glucose led to increases in activation of EGFR signaling but decreases in autophagy activity as indicated by decreased beclin-1 and inhibition of LC3B autophagosome formation as well as increased rubicon (an autophagy inhibitor) and SQSTM1 (autophagy substrate). (diabetesjournals.org)

At the far end of the glomerular capsule, opposite the glomerulus, is the mouth of the renal tubule. (innerbody.com)

Recent evidence suggests that the factor could be released by T-cells or B-cells, podocyte cell lines can be treated with plasma from patients with nephrotic syndrome to understand the specific responses of the podocyte to the circulating factor. (wikipedia.org)
when podocytes are lost for any reason they cannot be replaced by new cells. (scienceopen.com)

The aim of this study was therefore to evaluate the plasma levels of FABP3 at different stages of estimated glomerular filtration rate (eGFR) in patients with type 2 diabetes mellitus (T2DM). (medsci.org)

Podocytes make up the epithelial lining of Bowman's capsule, the third layer through which filtration of blood takes place. (wikipedia.org)
Podocytes are found lining the Bowman's capsules in the nephrons of the kidney. (wikipedia.org)

Other sites of abnormal cell vacuolization include the renal glomerular podocytes and in the fibroblasts of the liver's periportal spaces. (medscape.com)

One of the most exciting developments in recent years in understanding the pathophysiology of nephrotic syndrome has occurred in the area of podocyte biology (see the image below). (medscape.com)

Podocyte harm, one other key issue related to proteinuria in IgAN, has additionally attracted rising consideration. (holliseden.com)

Another third of patients will have persistent low-grade proteinuria but long-term preservation of glomerular filtration function-that is, normal serum creatinine. (renalandurologynews.com)

Anatomy28

Organisms17

Diseases29

Chemicals and Drugs114

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes37

Disciplines and Occupations1

Information Science1

Basement15

Endothelial cells1

Segmental Glomerular Sclerosis4

Barrier13

Human podocyte cell line2

Wrap around the glomerular capillaries1

Capillary1

Diabetic Nephropathy5

Epithelial cell1

Protein11

Regulates the podocyte1

Diseases6

Mesangial1

Conditionally1

Visceral epithelial cells1

MTORC1 activation1

Dysfunction2

Foot processes4

Albuminuria1

Urine5

Electron microscopy2

Capillaries3

Kidney Disease1

FSGS1

Glomerulosclerosis3

Injury11

Epithelium2

Tubular7

Urinary1

TRPC62

Expression4

Mice1

Antigen2

Filtration slits1

Progression2

High glucose3

Glomerulus1

Cells2

EGFR1

Bowman's2

Cell1

Nephrotic syndrome1

Harm1

Persistent1