• Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from a somatic cell. (wikipedia.org)
  • citation needed] Since iPSCs can be derived directly from adult tissues, they not only bypass the need for embryos, but can be made in a patient-matched manner, which means that each individual could have their own pluripotent stem cell line. (wikipedia.org)
  • dubious - discuss] In his Nobel seminar, Yamanaka cited the earlier seminal work of Harold Weintraub on the role of myoblast determination protein 1 (MyoD) in reprogramming cell fate to a muscle lineage as an important precursor to the discovery of iPSCs. (wikipedia.org)
  • iPSCs are typically derived by introducing products of specific sets of pluripotency-associated genes, or "reprogramming factors", into a given cell type. (wikipedia.org)
  • Unlike the first generation of iPSCs, these second generation iPSCs produced viable chimeric mice and contributed to the mouse germline, thereby achieving the 'gold standard' for pluripotent stem cells. (wikipedia.org)
  • Differentiated human induced pluripotent stem cells (iPSCs) following differentiation protocol. (cellimagelibrary.org)
  • The discovery in 2006 that human and mouse fibroblasts could be reprogrammed to generate induced pluripotent stem cells (iPSCs) with qualities remarkably similar to embryonic stem cells has created a valuable new source of pluripotent cells for drug discovery, cell therapy, and basic research. (thermofisher.com)
  • Our products have been an integral part of induced pluripotent stem cell research from the initial discovery of iPSCs to current breakthroughs. (thermofisher.com)
  • Induced pluripotent stem cells, iPSCs, are mature, differentiated cells, such as skin cells, that are reprogrammed in the laboratory to be similar to undifferentiated embryonic stem cells. (jove.com)
  • To create iPSCs, mature cells, like skin fibroblasts, are taken from a patient and put into culture. (jove.com)
  • These cells are also pluripotent like embryonic stem cells-able to produce all cell types-and are therefore called induced pluripotent stem cells (iPSCs). (jove.com)
  • iPSCs are potentially valuable in medicine, because a patient who needs a particular cell type-for instance, someone with a damaged retina due to macular degeneration-could receive a transplant of the required cells, generated from another cell type in their own body. (jove.com)
  • To create iPSCs, mature cells such as skin fibroblasts or blood cells from a person are grown in culture. (jove.com)
  • It is still being studied whether iPSCs are truly equivalent to embryonic stem cells, but they appear to be similar and can produce cells from all three germ layers of the body. (jove.com)
  • As with other types of stem cells, scientists are learning how to promote the differentiation of specific cell types from iPSCs efficiently, so that the needed cell types can be produced in adequate quantities. (jove.com)
  • In previous studies, induced pluripotent stem cells (iPSCs) differentiated into imDCs, and sinomenine (SN) was used to inhibit the maturation of imDCs. (newswise.com)
  • The effects of iPSCs-imDCs and SN-iPSCs-imDCs on T-cell stimulatory function, and regulatory T (Treg) cell proliferative function in vitro were analyzed by mixed lymphocyte reaction. (newswise.com)
  • This study focused on exploring sinomenine (SN) to promote differentiation of induced pluripotent stem cells (iPSCs) into imDCs (SN-iPSCs-imDCs), and found SN-iPSCs-imDCs with worse donor-specific T-cell stimulatory function, and higher regulatory T-cell proliferative function in vitro and in vivo to induce high immune tolerance. (newswise.com)
  • This microscopic image shows a cross section from a teratoma, generated in the lab by authors of a June 9 study in Stem Cell Reports that tested the quality of induced pluripotent stem cells (iPSCs). (neurosciencenews.com)
  • Teratomas, benign tumors containing the developing cells of different body parts, allowed researchers to see if the iPSCs could form the body's three basic germ cell lines - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)
  • In a study published June 9 by the journal Stem Cell Reports , and funded by the National Heart, Lung, and Blood Institute, the multi-institutional research team reports on the comprehensive characterization of a large set of induced pluripotent stem cells (iPSCs). (neurosciencenews.com)
  • Specialized iPSCs are reprogrammed from adult skin or infant cord blood cells and can become any cell type in the body - a condition called pluripotency that mimics the function of human embryonic stem cells (hESCs). (neurosciencenews.com)
  • Although the technology to produce safe and effective iPSCs exists, study authors report they encountered an unexpected number of wobbly production processes for the cells. (neurosciencenews.com)
  • Researchers also compared the molecular and functional characteristics of iPSCs to human embryonic stem cells, which are used sparingly as a gold standard for benchmarking quality. (neurosciencenews.com)
  • Being pluripotent means iPSCs can generate cells from each of the three basic germ cell lines that form the body - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)
  • Unlike embryonic stem cells, which are also pluripotent, iPSCs do not require destruction of an early embryo but can be derived from almost any cell in the body. (thebeefsite.com)
  • Unlike iPSCs in other species, the bovine cells did not require viruses to carry pluripotency-inducing genes into cells. (thebeefsite.com)
  • Therefore, mMSCs were reprogrammed into induced pluripotent stem cells (iPSCs) by non-integrative chromosomal technologies and differentiated into corresponding iP-MSCs. (uni-muenchen.de)
  • To address these questions, particularly in a human-relevant system, we have focused upon the use of human induced pluripotent stem cells (iPSCs) to assess their ability to give rise to RGCs, with a particular emphasis upon ipRGC differentiation. (indianactsi.org)
  • We found that iPSCs can be effectively differentiated into retinal cells, including the formation of 3D retinal organoids. (indianactsi.org)
  • Moreover, compared to their respective cells, iPSCs and iPSC EVs share a greater proportion of proteins, while the PD-MSC proteome appears to be more specific. (unlp.edu.ar)
  • Neuronal cells expressing pro-opiomelanocortin (POMC), neuropeptide-Y/agouti-related protein (NPY/AgRP) were generated from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) obtained from patients with monogenic forms of obesity. (columbia.edu)
  • The iPSC technology was pioneered by Shinya Yamanaka and Kazutoshi Takahashi in Kyoto, Japan, who together showed in 2006 that the introduction of four specific genes (named Myc, Oct3/4, Sox2 and Klf4), collectively known as Yamanaka factors, encoding transcription factors could convert somatic cells into pluripotent stem cells. (wikipedia.org)
  • It is also clear that pro-mitotic factors such as C-MYC/L-MYC or repression of cell cycle checkpoints, such as p53, are conduits to creating a compliant cellular state for iPSC reprogramming. (wikipedia.org)
  • Induced pluripotent stem cells represent one of the most promising research advances within the past decade, making this a valuable report for both executives and investors to use to optimally position themselves to sell iPSC products. (prnewswire.com)
  • Other iPSC-based models affecting neural crest lineages include Schwann cell and melanocyte-related disorders, including our effort to model human melanoma in pluripotent stem cells. (mskcc.org)
  • In a multilab collaboration, we have developed methods to model primary herpes simplex encephalitis (HSE) using iPSC cell technology. (mskcc.org)
  • We are particularly focused on improving the iPSC-based tools for modeling late-onset disorders such as Parkinson's disease using state-of-the-art genetic tools for gene repair, cell purification, and manipulation of cell maturation and age. (mskcc.org)
  • The ability to induce age-like features in PD-iPSC-derived dopamine nerve cells has yielded late-stage disease phenotypes not commonly observed in standard PD-iPSC models. (mskcc.org)
  • Here we performed a systematic analysis of two different induced pluripotent stem cell lines (iPSC 3.4 and 4.1) and an embryonic stem cell (ESC) line-derived cardiac myocytes at two different developmental stages. (nih.gov)
  • Our complete portfolio of stem cell research products and services provides optimized tools for each step of the stem cell workflow: from reprogramming somatic cells to iPSC expansion, validation, and differentiation. (thermofisher.com)
  • Reprogramming of somatic cells for iPSC generation can be accomplished with a number of technologies. (thermofisher.com)
  • Analyze stem cells and their derivatives using a wide range of cellular and molecular characterization methods, as validation is critical in iPSC research. (thermofisher.com)
  • It gives scientists open access to data from the study to support their own research into potential iPSC-based stem cell therapies. (neurosciencenews.com)
  • It also is vital that - prior to being instructed to become a specific cell type - iPSC lines continuously renew and expand in a blank slate form without the introduction of genetic errors. (neurosciencenews.com)
  • How well the 58 iPSC lines met quality criteria depended on the origin of the reprogrammed cells (skin vs. blood, male vs. female) and specific reprogramming methods. (neurosciencenews.com)
  • Pluripotency can be tested to determine if iPSC lines are able to form what are called teratomas - benign tumors made up of different cell types (teeth, bone, brain, etc. (neurosciencenews.com)
  • It had been thought that poor quality iPSC lines could not be pluripotent, Lutzko said, although until the current study this theory had not been carefully tested because of the expense. (neurosciencenews.com)
  • We found that differentiating induced pluripotent stem cells (iPSC) to DA neuron progenitors followed by transplantation into the rat brain may enable differentiation into DA neurons. (suny.edu)
  • Next, we compared both datasets and found that while iPSC EVs enclose proteins that modulate RNA and microRNA stability and protein sorting, PD-MSC EVs are rich in proteins that organize extracellular matrix, regulate locomotion, and influence cell-substrate adhesion. (unlp.edu.ar)
  • Nkx2.1 progenitor cells at 12 days of differentiation from iPSC integrated into the hypothalamus following injection into the lateral ventricle of NSG mice. (columbia.edu)
  • Here, we summarize how the m.3243G mtDNA mutation affects mitochondrial function in different tissues using iPSC and iPSC-differentiated cell type disease models and what significant findings have been replicated in the independent studies. (bvsalud.org)
  • Through this brief review and with a focus on mitochondrial dysfunction in iPSC-differentiated cell types, namely fibroblast , neuron , and retinal pigment epithelium cells , we aim to bring awareness of hiPSC as a robust mitochondrial disease model even if many unanswered questions remain. (bvsalud.org)
  • Chad and I have both been principal investigators on the induced pluripotent stem cell (IPSC) grant. (medscape.com)
  • The most well-known type of pluripotent stem cell is the embryonic stem cell. (wikipedia.org)
  • Patient-matched embryonic stem cell lines can now be derived using somatic cell nuclear transfer (SCNT). (wikipedia.org)
  • They hypothesized that genes important to embryonic stem cell (ESC) function might be able to induce an embryonic state in adult cells. (wikipedia.org)
  • Thus, induced pluripotent stem cells represent a promising combination of adult and embryonic stem cell characteristics. (prnewswire.com)
  • Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes. (ca.gov)
  • Obviously, it's a different cell type: it is neither a embryonic stem cell nor an adult stem cell. (scitizen.com)
  • Nanog and transcriptional networks in embryonic stem cell pluripotency. (bdbiosciences.com)
  • For their virus-free approach, AgResearch scientists simply incubated skin cells in plasmid DNA encoding the pluripotency factors - and watched the cells reprogramme back into an embryonic stem cell-like state. (thebeefsite.com)
  • We propose a computational model of the embryonic stem cell network, in which a core set of transcription factors (TFs) interact with each other and are induced by external factors. (lu.se)
  • Media of known composition that maintain human embryonic stem (hES) cells have been developed, but finding chemically defined, robust substrata has proven difficult. (nih.gov)
  • With an acronym like "HipSci," the Human Induced Pluripotent Stem Cell Initiative certainly sounds cool. (genengnews.com)
  • The standard tests for LQTS use animal models or hamster cells that express human heart genes at high levels. (ca.gov)
  • Unfortunately, cardiac physiology in animal models (rabbits and dogs) differs from that in humans, and hamster cells lack many key features of human heart cells. (ca.gov)
  • Human embryonic stem cells (hESCs) can be differentiated into heart cells, but we do not know the culture conditions that would make the assay most similar to LQTS in a living person. (ca.gov)
  • These problems could be solved if we had a method to grow human heart cells from people with genetic LQTS mutations, so that we know the exact test conditions that would reflect the human disease. (ca.gov)
  • Our long-term goal is to develop a panel of iPS cell lines that better represent the genetic diversity of the human population. (ca.gov)
  • In this video, Canadian scientist Dr. Mick Bhatia explains how induced pluripotent stem cells (iPS cells) are created and how they can be used to create new therapies for human disease. (eurostemcell.org)
  • Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)
  • Derivation and differentiation of haploid human embryonic stem cells. (nature.com)
  • Zhang, MD, PhD, S.-C. Neural Subtype Specification from Human Pluripotent Stem Cells . (wisc.edu)
  • CaMKII inhibition in human primary and pluripotent stem cell-derived chondrocytes modulates effects of TGFbeta and BMP through SMAD signaling. (ca.gov)
  • Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes. (ca.gov)
  • Differentiation of human and murine induced pluripotent stem cells to microglia-like cells. (alzforum.org)
  • Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. (stemcell.com)
  • Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
  • Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
  • While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
  • The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
  • We aimed to produce highly homogeneous induced pluripotent stem cell-derived mesenchymal stem cells (iP-MSCs) in an autologous manner from initially isolated human mucosa mesenchymal stem cells (mMSCs) of the upper respiratory tract. (uni-muenchen.de)
  • The human body is made up of about 220 different kinds of specialized cells such as nerve cells, muscle cells, fat cells and skin cells. (benthamscience.com)
  • Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
  • We developed a model of the aberrant epithelial reprogramming observed in IPF using alveolar organoids derived from human-induced pluripotent stem cells stimulated with a cocktail of pro-fibrotic and inflammatory cytokines. (lu.se)
  • We have a new publication out ( direct link ), Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells authored by Karl Wahlin , Julien Maruotti , Srinivasa Sripathi , John Ball, Juan Angueyra, Catherin Kim, Rhonda Grebe, Wei Li , myself and Don Zack . (utah.edu)
  • Development of reliable methods to culture complex retinal tissues from human pluripotent stem cells (hPSCs) could offer a means to study human retinal development, provide a platform to investigate the mechanisms of retinal degeneration and screen for neuroprotective compounds, and provide the basis for cell-based therapeutic strategies. (utah.edu)
  • We aim to develop a cell culture platform to automatically differentiate human induced pluripotent stem cells (hiPSCs) for lab-on-a chip applications. (dim-elicit.fr)
  • Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
  • Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
  • Human embryonic stem cells also exhibit the Warburg effect ( 15 ). (spandidos-publications.com)
  • Human pluripotent stem cell-derived dopamine neurons after transplantation into the brain of a Parkinsonian monkey. (sloankettering.edu)
  • Based on those results, we are currently pursuing the development of a clinical-grade dopamine neuron cell product and are in the process of generating the data necessary for an IND (investigational new drug) application from the FDA, which is required for the first human clinical use of human ESC-derived dopamine neurons. (sloankettering.edu)
  • In addition to pursuing the first clinical application of human ESC-derived dopamine neurons, we continue to work on detailing the mechanisms of graft function by implementing novel tools such as optogenetics, a technology that enables "switching off" or "switching on" grafted cells in freely moving animals . (sloankettering.edu)
  • This small (3-4 mm in thickness in human) neuro-endocrine brain region, located just above the median eminence, is comprised of cell types that subserve specific metabolic and behavioral aspects of the control of body weight, as well as hepatic glucose production, body temperature, autonomic physiology, neuroendocrine axes, serum osmolarity and circadian rhythms. (columbia.edu)
  • however, human hypothalamic cells are largely inaccessible for direct study. (columbia.edu)
  • My thesis research focused on establishing an in vitro model for understanding the molecular neurophysiology of obesity using, as 'proof-of-principle', neurons derived from human pluripotent stem cells (hPSCs) derived from individuals with monogenic forms of obesity. (columbia.edu)
  • Are you new to working with human pluripotent stem cells? (lu.se)
  • The emergence of human induced pluripotent stem cell (hiPSC) technology has allowed researchers to work with SZ patient-derived neuronal and glial cell types in vitro and to investigate the molecular basis of the disorder in a human neuronal context. (lu.se)
  • We discuss the pros and cons of these models and describe the potential of using such models for deciphering the contribution of specific human neural cell types to the development of the disease. (lu.se)
  • Mitochondrial dysfunction characterized in human induced pluripotent stem cell disease models in MELAS syndrome: A brief summary. (bvsalud.org)
  • Human induced pluripotent stem cells (hiPSCs) for MELAS syndrome ( mitochondrial myopathy , encephalopathy , lactic acidosis , stroke -like episodes) may allow deeper understanding of how tissue -specific mitochondrial dysfunction result in multi-systemic disease . (bvsalud.org)
  • I fell in love with the idea of human pluripotent stem cells and started surveying the United States and even Europe for who was playing in that area. (medscape.com)
  • 5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
  • Because the embryos then lose the ability to grow into a complete human being, the use of stem cells from embryos is controversial. (msdmanuals.com)
  • Here, we characterize SpCas9 targeting specificity in human cells to inform the selection of target sites and avoid off-target effects. (cdc.gov)
  • Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • DBS is a widely used FDA-approved treatment and stem cell-derived dopamine neuron replacement has now evolved to the first in-human clinical trials. (lu.se)
  • Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)
  • However, despite the common ability of hiPSCs and hESCs to differentiate into all 3 germ layers, their functional equivalence at the single cell level remains to be demonstrated. (jci.org)
  • Here, we used single cell analysis to resolve the gene expression profiles of 362 hiPSCs and hESCs for an array of 42 genes that characterize the pluripotent and differentiated states. (jci.org)
  • Comparison between single hESCs and single hiPSCs revealed markedly more heterogeneity in gene expression levels in the hiPSCs, suggesting that hiPSCs occupy an alternate, less stable pluripotent state. (jci.org)
  • Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
  • We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a robotic liquid handling unit that delivers footprint-free hiPSCs within 3 weeks with state-of-the-art efficiencies. (frontiersin.org)
  • Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
  • Our HiPSCs display classic pluripotent stem cell morphology, with a high nucleus to cytoplasm size ratio, as well as they are amenable to be cultivated in serum-free media, independent of feeder cells and of feeder-conditioned media as colonies or high density monolayers. (cellapplications.com)
  • Post-thawing viability of HiPSCs is typically higher than 70%, and HiPSC have demonstrated coherent pluripotent behavior over more than 60 passages. (cellapplications.com)
  • In addition, their "unspecialized" nature allows them to differentiate into a wide variety of specialized cell types. (prnewswire.com)
  • While the appeal of embryonic cells has been their ability to differentiate into any type of cell, there has been significant ethical, moral, and spiritual controversy surrounding their use. (prnewswire.com)
  • These iPS cells differentiate into beating cardiomyocytes. (ca.gov)
  • You and your colleagues found amniotic fluid-derived (AFS) stem cells to have the potential to differentiate into muscle, bone, fat, blood vessel, nerve and liver cells. (scitizen.com)
  • However, scientists have found a way to reprogram these mature cells so that they "de-differentiate" and return to an unspecialized, proliferative state. (jove.com)
  • Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
  • Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
  • HiPSC differentiate into cell derivatives from the 3 embryonic layers: Neuronal marker beta III tubulin (TUJ1), Smooth Muscle Actin (SMA) and Hepatocyte Nuclear Factor 3 Beta (HNF3b). (cellapplications.com)
  • A computational approach can be used as a framework to explore the dynamics of a simplified network of the ESC with the aim to understand how stem cells differentiate and also how they can be reprogrammed from somatic cells. (lu.se)
  • Stem cell-derived cardiac myocytes are potential sources for testing cardiocytoprotective molecules against ischemia/reperfusion injury in vitro. (nih.gov)
  • Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques. (stemcell.com)
  • Retinal ganglion cells can be purified and matured in vitro to generate models for optic neuropathies. (indianactsi.org)
  • This project was designed to establish an in vitro model for studying hypothalamic cell-molecular physiology in neurons derived from hPSCs. (columbia.edu)
  • Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. (nature.com)
  • Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
  • Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases (such as Krabbe disease), including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses as well as peroxisome disorders such as X-linked adrenoleukodystrophy. (medscape.com)
  • Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)
  • Some evidence indicates that at least in certain disorders, combination ERT and hematopoietic stem cell transplantation together might be superior to hematopoietic stem cell transplantation alone in patients who are appropriate candidates. (medscape.com)
  • The availability of both ERT and hematopoietic stem cell transplantation has prompted ongoing consideration of newborn screening efforts to diagnose lysosomal storage diseases. (medscape.com)
  • Stem cell transplantation is the removal of stem cells (undifferentiated cells) from a healthy person and their injection into someone who has a serious blood disorder. (msdmanuals.com)
  • Overview of Transplantation Transplantation is the removal of living, functioning cells, tissues, or organs from the body and then their transfer back into the same body or into a different body. (msdmanuals.com)
  • But researchers think that these stem cells have the most potential for producing different kinds of cells and for surviving after transplantation. (msdmanuals.com)
  • These stem cells are most often used for transplantation. (msdmanuals.com)
  • The possibilities arising from these characteristics have resulted in great commercial interest, with potential applications ranging from the use of stem cells in reversal and treatment of disease, to targeted cell therapy, tissue regeneration, pharmacological testing on cell-specific tissues, and more. (prnewswire.com)
  • Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
  • Unguided differentiation confirms HiPSC ability to generate cell derivatives of tissues arising from the three embryonic layers. (cellapplications.com)
  • Researchers hope to use stem cells to repair or replace cells or tissues damaged or destroyed by such disorders as Parkinson disease, diabetes, and spinal injuries. (msdmanuals.com)
  • These unlimited supplies of autologous cells could be used to generate transplants without the risk of immune rejection. (wikipedia.org)
  • The proposed candidate is a universal cell therapy designed to generate new articular cartilage in these defects and interrupt the cycle of degeneration. (ca.gov)
  • Finally an outline of disease-specific pluripotent stem cells is reviewed within the context of their capacity to generate the cell type of interest. (edu.au)
  • We are interested in applying our ability to generate disease-relevant cell types and in establishing clinical-grade protocols for the development of cell therapy approaches in other CNS and PNS disorders. (sloankettering.edu)
  • Upon introduction of reprogramming factors, cells begin to form colonies that resemble pluripotent stem cells, which can be isolated based on their morphology, conditions that select for their growth, or through expression of surface markers or reporter genes. (wikipedia.org)
  • hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)
  • Fig. 2: Analysis of cell-essential genes. (nature.com)
  • Then, typically, genes for transcription factors, are delivered by viral vectors into the cell nuclei, where they are incorporated into the genome. (jove.com)
  • The transcription factors then turn on genes that are expressed by embryonic stem cells. (jove.com)
  • Then, genes for multiple transcription factors are delivered into the cells using a viral vector, and the transcription factor proteins are expressed using the cell's machinery. (jove.com)
  • The transcription factors then turn on many other genes that are expressed by embryonic stem cells, returning the cells to an undifferentiated, proliferative, and pluripotent state. (jove.com)
  • This includes some cell lines contaminated with bacteria or carrying genes and mutations associated with cancer. (neurosciencenews.com)
  • The cells were generated with a variety of genes, methods and cells of origin, such as adult skin or infant cord blood cells. (neurosciencenews.com)
  • The different methods included a variety of reprogramming genes, vectors (engineered viruses that deliver genetic material to cells), or the use of plasmids (small DNA molecules that can deliver reprogramming genes). (neurosciencenews.com)
  • The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)
  • While t(8;21) is important for leukemia development, a mutation in one or more additional genes is typically needed for the myeloid blasts to develop into cancerous leukemia cells. (medlineplus.gov)
  • By triggering certain genes, researchers may be able to cause the stem cells to specialize and become the cells that need to be replaced. (msdmanuals.com)
  • One way to induce these cells is to inject them with material that affects their genes, a process called reprogramming. (msdmanuals.com)
  • The fibroblasts were engineered so that any cells reactivating the ESC-specific gene, Fbx15, could be isolated using antibiotic selection. (wikipedia.org)
  • The production of iPS cells from dermal fibroblasts has generated intense interest in the utility of such cells for research purposes and clinical applications. (cam.ac.uk)
  • An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. (nature.com)
  • We then made sure the cells had the specific characteristics of stem cells that we now have come to realize as a scientific community. (scitizen.com)
  • Essentially, it's just an additional stem cell line that has some characteristics that will hopefully be useful clinically someday. (scitizen.com)
  • What we've identified has characteristics of both, so as such, it's going to have different characteristics and different implications than other cell types. (scitizen.com)
  • We demonstrated that mMSCs and iP-MSCs show similar cell characteristics in terms of morphology, clonogenic potential, differentiation, and surface phenotype. (uni-muenchen.de)
  • Importantly, these cells displayed hypothalamic neuronal characteristics, including production and secretion of neuropeptides and responsiveness to metabolic hormones such as insulin and leptin. (columbia.edu)
  • Because they can propagate indefinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease. (wikipedia.org)
  • We have tested many types of stem cells over the years as a potential source of dopamine neurons. (sloankettering.edu)
  • Collaborative studies are ongoing to explore the potential of hPSC derivatives such as oligodendrocytes, Schwann cell precursors, and peripheral neurons. (sloankettering.edu)
  • These hypothalamic-like neurons accounted for over 90% of differentiated cells and exhibited transcriptional profiles characteristic of specific hypothalamic neurons (and explicitly lacking pituitary markers). (columbia.edu)
  • Single cell transcriptome analysis of day 27 hESC-derived hypothalamic neurons enabled us to identify specific hypothalamic cell types (e.g. (columbia.edu)
  • Using stem cell-derived hypothalamic neurons to investigate the neurophysiology of obesity caused by prohormone convertase 1/3 deficiency (Chapter 3). (columbia.edu)
  • HiPSC-derived Neural Stem Cells (L) and Neurons (R) . i-HNSC stained w/ Nestin (neural stem cell marker, green), SOX 2 (stem cell marker, red) & DAPI (nuclear stain, blue). (cellapplications.com)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • The use of stem cell-derived dopamine neurons or deep brain stimulation (DBS) represents two alternative approaches to treat Parkinson's Disease. (lu.se)
  • Wutz, A. Haploid mouse embryonic stem cells: rapid genetic screening and germline transmission. (nature.com)
  • Forward and reverse genetics through derivation of haploid mouse embryonic stem cells. (nature.com)
  • Traditionally, scientists have worked with both embryonic and adult stem cells for research tools, as well as for cellular therapy. (prnewswire.com)
  • Although some adult stem cells do have differentiation capacity, it is often limited in nature, which results in fewer options for use. (prnewswire.com)
  • Our objective is to produce a cell-based test for LQTS with induced pluripotent stem (iPS) cell technology, which allows adult cells to be "reprogrammed" to be stem cell-like cells. (ca.gov)
  • Adult Stem Cells: The New Plastic Surgery? (scitizen.com)
  • The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
  • Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS. (bvsalud.org)
  • Cells were initially edited by the CRISPR/Cas9 system to express the Thy1 gene at the Brn3b locus and the tdTomato gene at the melanopsin locus. (indianactsi.org)
  • Using signal inhibition, we induced stem cell markers in bovine skin cells. (thebeefsite.com)
  • Moreover, iP-MSCs demonstrated related immunosuppressive capacity as mMSCs including the secretion of cytokines, and T cell inhibition. (uni-muenchen.de)
  • Hence, both gene delivery technology and iPS cell characterization and subsequent cell differentiation are critical aspects of iPS cell biology. (cam.ac.uk)
  • Part of the Progenitor Cell Biology Consortium, the scientists are working to make sure this growing area of medical research is grounded in safe and sound science. (neurosciencenews.com)
  • More recently, advances in medical biology have shown that the genomic state of a somatic cell can be altered or 'reprogrammed' to become pluripotent. (edu.au)
  • We will characterize iPS cells with well-defined mutations that have clinically proven responses to drugs that cause LQTS. (ca.gov)
  • What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)
  • Since the virus does not go through a DNA phase, its genetic material and transgenes do not integrate into the host cell genome. (cellapplications.com)
  • Both crRNA-tracrRNA duplexes and sgRNAs can be used to target SpCas9 for multiplexed genome editing in eukaryotic cells 1 , 3 . (cdc.gov)
  • Scientists are developing ways of enabling (inducing) other cells (such as a blood or skin cell) to act as stem cells. (msdmanuals.com)
  • By immunostaining for cell type specific markers, I have documented the different stages of retinal differentiation and provided insight into the mechanisms of ipRGC differentiation. (indianactsi.org)
  • It was very surprising to us the high number of unstable cell lines identified in the study, which highlights the importance of setting safety standards for stem cell therapies," said Carolyn Lutzko, PhD, senior author and director of translational development in the Translational Core Laboratories at Cincinnati Children's Hospital Medical Center. (neurosciencenews.com)
  • A good number of the cell lines we studied met quality standards, although the unexpected number of lines that did not meet these standards could not be used for clinical therapies. (neurosciencenews.com)
  • This full potentiality makes them particularly suitable for developing cell replacement therapies or establishing cellular model systems. (edu.au)
  • This brings as oral dopamine therapies with the advantage that with it continued infective risks for the patients the cells can be targeted to the site where dopamine with DBS which is not so for patients with trans- is most needed, namely the striatum. (lu.se)
  • In particular, it plays an important role in development of hematopoietic stem cells, early blood cells that have the potential to develop into all types of mature blood cells such as white blood cells, red blood cells, and platelets. (medlineplus.gov)
  • The symposium was funded by the Royal Physiographic Society and its partners at Lund University Cancer Center, Lund Stem Therapy, Lund Stem Cell Center and the Geological Department. (lu.se)
  • Deconvolution of RNA-seq data of alveolar organoids indicated that the fibrosis cocktail rapidly increased the proportion of transitional cell types including the KRT5-/KRT17+ aberrant basaloid phenotype recently identified in the lungs of. (lu.se)
  • However, there are genetic forms of LQTS that should allow us to make iPS cell-derived heart cells that have the key features of LQTS. (ca.gov)
  • These studies will provide the foundation for an expanded panel of iPS cell lines from people with other genetic mutations and from people who have no genetically defined risk factor but still have potentially fatal drug-induced LQTS. (ca.gov)
  • Genetic modification and screening in rat using haploid embryonic stem cells. (nature.com)
  • 2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
  • We offer choices in integration-free reprogramming technologies and services that fit your research and translational needs including our Cell Therapy Systems (CTS) Products, which are designed for clinical and translational research applications. (thermofisher.com)
  • Which stem cell reprogramming technology is right for you? (thermofisher.com)
  • Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
  • Of particular relevance is to elucidate those molecular interactions which govern the reprogramming of somatic cells into ESC. (lu.se)
  • Our model also hosts reprogramming of a committed cell into an ESC by over-expressing OCT4. (lu.se)
  • Furthermore, the model (iv) provides a framework for reprogramming from somatic cells and conveys an understanding of reprogramming efficiency as a function of OCT4 over-expression. (lu.se)
  • However, these cells have limitations, such as rapid maturation and a short lifespan in vivo . (newswise.com)
  • A multimodal therapeutic approach involving radiotherapy is required when treating head and neck squamous cell carcinoma. (uni-muenchen.de)
  • Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
  • Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for MSCs in future cell-free-based therapeutic approaches. (unlp.edu.ar)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • This change in gene activity blocks the maturation (differentiation) of blood cells and leads to the production of abnormal, immature white blood cells called myeloid blasts. (medlineplus.gov)
  • These stem cells can produce different types of blood cells. (msdmanuals.com)
  • These stem cells can produce only blood cells. (msdmanuals.com)
  • Stem cell research and experimentation have been in process for well over five decades, as stem cells have the unique ability to divide and replicate repeatedly. (prnewswire.com)
  • The third in a series of short (around 1 minute) animated films that answer basic questions about stem cell research. (eurostemcell.org)
  • From feeder-based and feeder-free media to optimized passaging reagents and substrates to media for stem cell therapy research, Gibco products enable you to culture with confidence. (thermofisher.com)
  • Survey Report: Where is Pluripotent Stem Cell Research Now? (stemcell.com)
  • We offer a new course containing substantial hand-on practice in the cell lab as well as seminars covering topics such as history, growth, quality controls and how they can be used in research and the clinic. (lu.se)
  • The aim of this 5-day course is to get a better theoretical and practical understanding of the pluripotent stem cells, their culture requirements and extensive potential for use in modern research and medicine. (lu.se)
  • At International Society for Stem Cell Research-the big stem cell meeting-Yamanaka revealed one more factor. (medscape.com)
  • Moreover, single cell heterogeneity amongst stem cell populations may underlie important cell fate decisions. (jci.org)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
  • However, only recently have we resolved some of the major challenges in generating the correct nerve cell type at sufficient scale and purity. (sloankettering.edu)
  • Using this novel differentiation strategy we have demonstrated that lab-grown dopamine nerve cells can efficiently engraft and function in mouse, rat or monkey models of PD. (sloankettering.edu)
  • Stem cells are undifferentiated cells that have the potential to become one of 200 types of cells in the body, including blood, nerve, muscle, heart, glandular, and skin cells. (msdmanuals.com)
  • Others are already partially differentiated and can only become, for example, some kinds of nerve cell. (msdmanuals.com)
  • Conditions such as Huntington's disease, Parkinson's disease, and spinal cord injuries are examples of clinical applications in which stem cells could offer benefits in halting or even reversing damage. (prnewswire.com)
  • Celebrated by the 2012 Nobel Prize in Medicine, a type of cell known as an " induced pluripotent stem cell " or "iPS cell" has important implications for the emerging field of biomedical technology. (eurostemcell.org)
  • We are just describing a new type of cell. (scitizen.com)
  • That is, they divide and are pluripotent, able to produce any cell type in the body. (jove.com)
  • The cells can now divide, and ideally, be directed to produce a specific cell type in culture. (jove.com)
  • Ordinarily, cells that have differentiated into a specific cell type are post-mitotic-that is, they no longer divide. (jove.com)
  • This is a significant leap forward for obtaining patient-specific pluripotent stem cells that can then be differentiated to the cell type of interest. (edu.au)