Pluripotent stem stem cell differentiation differentiation of cells. Medical search. Frequent questions

Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
Could extracellular vesicles derived from mesenchymal stem cells be a potential therapy for acute pancreatitis-induced cardiac injury? (wjgnet.com)
Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)

Gastric and small intestinal organoids differentiated from human pluripotent stem cells (hPSCs) have revolutionized the study of gastrointestinal development and disease. (bioregate.com)

Induced pluripotent stem cells, iPSCs, are mature, differentiated cells, such as skin cells, that are reprogrammed in the laboratory to be similar to undifferentiated embryonic stem cells. (jove.com)
To create iPSCs, mature cells, like skin fibroblasts, are taken from a patient and put into culture. (jove.com)
These cells are also pluripotent like embryonic stem cells-able to produce all cell types-and are therefore called induced pluripotent stem cells (iPSCs). (jove.com)
iPSCs are potentially valuable in medicine, because a patient who needs a particular cell type-for instance, someone with a damaged retina due to macular degeneration-could receive a transplant of the required cells, generated from another cell type in their own body. (jove.com)
To create iPSCs, mature cells such as skin fibroblasts or blood cells from a person are grown in culture. (jove.com)
It is still being studied whether iPSCs are truly equivalent to embryonic stem cells, but they appear to be similar and can produce cells from all three germ layers of the body. (jove.com)
As with other types of stem cells, scientists are learning how to promote the differentiation of specific cell types from iPSCs efficiently, so that the needed cell types can be produced in adequate quantities. (jove.com)
In previous studies, induced pluripotent stem cells (iPSCs) differentiated into imDCs, and sinomenine (SN) was used to inhibit the maturation of imDCs. (newswise.com)
The effects of iPSCs-imDCs and SN-iPSCs-imDCs on T-cell stimulatory function, and regulatory T (Treg) cell proliferative function in vitro were analyzed by mixed lymphocyte reaction. (newswise.com)
This study focused on exploring sinomenine (SN) to promote differentiation of induced pluripotent stem cells (iPSCs) into imDCs (SN-iPSCs-imDCs), and found SN-iPSCs-imDCs with worse donor-specific T-cell stimulatory function, and higher regulatory T-cell proliferative function in vitro and in vivo to induce high immune tolerance. (newswise.com)
Moreover, compared to their respective cells, iPSCs and iPSC EVs share a greater proportion of proteins, while the PD-MSC proteome appears to be more specific. (unlp.edu.ar)
To address these questions, particularly in a human-relevant system, we have focused upon the use of human induced pluripotent stem cells (iPSCs) to assess their ability to give rise to RGCs, with a particular emphasis upon ipRGC differentiation. (indianactsi.org)
We found that iPSCs can be effectively differentiated into retinal cells, including the formation of 3D retinal organoids. (indianactsi.org)
Human pluripotent stem cells, including ESCs and iPSCs, are highly sensitive cells and undergo apoptosis during these routine procedures. (nih.gov)
Differentiated human induced pluripotent stem cells (iPSCs) following differentiation protocol. (cellimagelibrary.org)

Immunocytochemistry and quantitative real-time polymerase chain reaction analysis demonstrated that neuronal differentiation of hiPSC-derived neuronal precursors was enhanced after treatment with 1-2 μg/ml RA-loaded PCANs. (edu.au)
Applying Expedition to single pluripotent stem cells undergoing neuronal differentiation, we discover that up to 20% of AS exons exhibit bimodality. (nih.gov)
In this study, we used iPSC-derived neural progenitor cells (NPCs) from individuals with ASD of known and unknown genetic cause as well as from neurotypical controls to examine the effects of exogenous IL-17a on NPC proliferation, migration and neuronal differentiation, and whether IL-17a and genetic risk factors for ASD interact exacerbating alterations in NPC function. (figshare.com)
In conclusion, our results suggest that exogenous IL-17a positively regulates the neuronal differentiation of human NPCs, which may disturb normal neuronal and synaptic development and contribute to MIA-related changes in brain function and behavior. (figshare.com)

However, these cells have limitations, such as rapid maturation and a short lifespan in vivo . (newswise.com)
We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
For identifying lineage conversion and maturation, we utilized conventional assays of cell morphology, gene expression analysis and lineage markers. (elsevierpure.com)
Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. (biomedcentral.com)
Ongoing work in the laboratory is focused on testing the hypothesis that hypomorphic mutations in the RAG genes that partially (but not completely) compromise T-cell development cause altered cross-talk with thymic stromal cells and by that mechanism also affect maturation and function of subsets of thymic epithelial cells involved in the process of negative selection of self-reactive T cells, thereby leading to increased risk of autoimmunity. (nih.gov)
A complex process that involves the proliferation of MEGAKARYOCYTES in BONE MARROW, their differentiation in size and ploidy, and maturation before the release of functional BLOOD PLATELETS. (bvsalud.org)

These studies led to our discovery of a novel type of pluripotent epiblast stem cell (EpiSC) from the late epiblast layer of mouse and rat embryos. (qscience.com)
One type of pluripotent cell, called a hematopoietic stem cell, can differentiate into a large variety of cells with different functions. (wikipedia.org)

We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. (edu.au)
My lab integrates traditional and single-nuclei RNA sequencing to elucidate transcriptome-wide changes to RNA metabolism in clinically relevant post-mortem brain tissue and human induced pluripotent stem cell (hiPSC) models of neurological disease including ALS and autism. (ed.ac.uk)

abstract = "Adoptive cell transfer (ACT) of antigen-specific CD8+ cytotoxic T lymphocytes (CTLs) is a promising treatment for a variety of malignancies 1. (psu.edu)
Abstract Background Cardiac differentiation of human-induced pluripotent stem (hiPS) cells consistently produces a mixed population of cardiomyocytes and non-cardiac cell types, even when using well-characterized protocols. (figshare.com)

These UCNPs allow programmable activation of two synergistic light-gated ion channels VChR1 and Jaws in the same cell to manipulate membrane polarization, demonstrated here for cardiac pacing. (nature.com)

Myelodysplastic syndromes Myelodysplastic Syndrome (MDS) are a group of clonal hematopoietic stem cell disorders unified by the presence of distinct mutations of hematopoietic stem cells. (msdmanuals.com)
50 × 10 9 /L) not caused by malignant transformation of a hematopoietic stem cell. (msdmanuals.com)

Here we report the differentiation of human colonic organoids (HCOs) from hPSCs. (bioregate.com)
Our investigations utilize in vivo mouse retina and human retinal organoids derived from pluripotent stem cells as study systems. (nih.gov)
Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells. (nih.gov)

Additionally, we found that substantial cell death occurs during differentiation in a manner that suggested all cells were equally likely to survive or die, suggesting that there is no intrinsic selection bias for cells descended from particular hiPS cell progenitors. (figshare.com)
We show that Zeb2 over-expression has a positive impact on skeletal muscle differentiation in pluripotent stem cells and adult myogenic progenitors. (unipv.it)
What are intrinsic control mechanisms that lead to photoreceptor cell fate from retinal progenitors? (nih.gov)

All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
Support the growth and differentiation of human pluripotent stem cells by using Vitronectin XF™, a defined and xeno-free cell culture matrix. (stemcell.com)

In recent studies we have examined the role of transforming growth factor family members in both pluripotency and differentiation. (qscience.com)
EpiSCs share many features with hESCs, and subsequent work supports our hypothesis that hESCs are the human counterparts of EpiSCs, with similar responses to growth factors and mechanisms of pluripotency and differentiation. (qscience.com)
Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
To get a new insight into pluripotency, we employed a plurifaceted experimental design combining expression proteomics with the proteome-wide integral solubility alteration (PISA) assay 7 to compare pluripotent cells with their isogenic progenies and parental cells as well as with allogeneic cells. (nature.com)
In stem cells exposed to signals from fetal cells, mechanistic gene networks of upstream regulators decreased pluripotency, while simultaneously inducing mesenchymal and epithelial properties. (elsevierpure.com)
Accordingly, human pluripotent stem cells (hPSCs) with the properties of self-renewal and pluripotency present an ideal cell model for the isolation of pericytes to study their development or the therapeutic effect in pericyte-related diseases. (researchsquare.com)
And while in human pluripotent stem cells, miRNAs have been directly linked to the core pluripotency network, including the cell cycle regulation and the maintenance of the self-renewing capacity, their role in the onset of differentiation in other contexts, such as determination of neural cell fate, remains poorly described. (muni.cz)
cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)

In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
Several academic and industry efforts are well under owned subsidiary Cyto Therapeutics, it had received way to produce dopaminergic neurons from stem approval by the Australian government to conduct a cells under conditions compliant with use in patients. (lu.se)
Without this, the patient cells lost in PD could be replaced by grafted community is left trying to interpret complex scien- immature human dopaminergic neurons [3, 5]. (lu.se)
The goal of our research is to develop stem cell-based therapies to replace lost neurons as a treatment for brain diseases. (lu.se)
With this knowledge at hand, we become better at controlling the differentiation of human pluripotent cells to subtype-specific neurons which can be used for disease modeling, drug screening, and transplantation, leading to new treatments for several types of brain disorders. (lu.se)
We aim to develop novel, lasting therapies for neurodegenerative diseases through the replacement of lost neurons in the brain with new neurons generated from stem cells. (lu.se)

However, the approach to obtain embryonic stem cells (ESCs) from patients is not feasible. (psu.edu)
In addition, like ESCs, iPS cells possess indefinite proliferative capacity in vitro, and have been shown to differentiate into hematopoietic cells. (psu.edu)
Thus, iPS cells have greater potential to be used in ACT-based immunotherapy compared to ESCs or HSCs. (psu.edu)
According to their potentiality, embryonic stem cells (ESCs) are classified as totipotent and pluripotent, both of them can differentiate into the three germ lines. (biomedcentral.com)
ESCs are obtained from inner cell mass, characterized by unlimited self-renewal and pluripotentiality, which allow them to differentiate into various specialized cells types in a morphologically and functionally way, characteristics that make them attractive for developing differentiated kidney cells (DKCs) [ 9 ]. (biomedcentral.com)
Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
Moreover, the combination of gene modification and directed differentiation of ESCs provides perfect tool for disease modelling and drug discovery. (benthamscience.com)
Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)

However, to our knowledge, there is no report on the successful generation of glucose-responsive insulin-producing beta-cells from hPSCs in vitro. (ca.gov)
The protocol, which spans five distinct stages, is an attempt to recapitulate the derivation of pancreatic beta-cells in vitro as they form in the developing embryo. (ca.gov)
Retinal ganglion cells can be purified and matured in vitro to generate models for optic neuropathies. (indianactsi.org)
Although the use of hematopoietic stem cells (HSCs) for therapeutic purposes has been widely applied in clinic 11-13 , HSCs have reduced differentiation and proliferative capacities, and HSCs are difficult to expand in in vitro cell culture 14-16 . (psu.edu)
Recent iPS cell technology and the development of an in vitro system for gene delivery are capable of generating iPS cells from patients without any surgical approach. (psu.edu)
Here, we present methods for the generation of T lymphocytes from iPS cells in vitro, and in vivo programming of antigen-specific CTLs from iPS cells for promoting cancer immune surveillance. (psu.edu)
Stimulation in vitro with a Notch ligand drives T cell differentiation from iPS cells, and TCR gene transduction results in iPS cells differentiating into antigen-specific T cells in vivo, which prevents tumor growth. (psu.edu)
In vitro, HCOs express colonic markers and contained colon-specific cell populations. (bioregate.com)
Here, we successfully derive pericyte-like cells with cranial neural crest (CNC) origin from hPSCs (designated as hPSC-CNC PCs), which may represent an ideal cell source for the treatment of pericyte dysfunction-related disorders and help to model the human BBB in vitro for the study of the pathogenesis of such neurological diseases. (researchsquare.com)
In this regard, tissue engineers have sought to leverage native mechanotransduction pathways to enhance in vitro stem cell. (biomedcentral.com)
Tissue engineering has developed many paradigms and techniques on how to best integrate cells and extracellular matrix to create in vitro structures that replicate native tissue. (biomedcentral.com)
Using a powerful in vitro platform, we have determined that the severity of clinical manifestations associated with mutations in the RAG, DCLRE1C, and LIG4 genes correlates with the residual activity of the mutant protein and with different degrees of perturbation of T- and B-cell repertoire diversity and composition. (nih.gov)
The comprehensive miRNA profiling presented here reveals novel sets of miRNAs differentially expressed during human neural cell fate determination in vitro. (muni.cz)
We demonstrate that silencing GMPPB inhibits the proliferation, migration, and invasion of GBM cells both in vitro and in vivo and that overexpression of GMPPB exhibits the opposite effects. (bvsalud.org)
In vitro experiments using NV virus-like particles (VLPs) directly showed NV VLP attachment to HBGA, resulting in VLP internalization into the cell ( 19 ). (cdc.gov)
From these critical studies, putative NV receptors were identified, and thus it was hypothesized that a successful in vitro cell culture system would most likely possess these receptors to support NV replication. (cdc.gov)
Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)

Our focus on mesoderm leads us on to molecular pathways for early human cardiomyocyte differentiation, with a goal of understanding the transcriptional networks responsible for cardiomyocyte identity and using this to generate more homogeneous cardiomyocyte populations for therapeutic applications and drug discovery. (qscience.com)
In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)
Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
We are now focused on delineating the transcription factors and signaling pathways that are responsible for generating photoreceptors from retinal progenitor cells. (nih.gov)
Can we find common cellular pathways associated with photoreceptor cell death caused by distinct genetic mutations? (nih.gov)

Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)

This work reveals the importance of BRACHYURY and CDX2 genes as key mediators of embryonic and extraembryonic lineage differentiation in hESCs and EpiSCs. (qscience.com)

In our most recent work we have focused on the role of bone morphogenetic protein (BMP)-4 in the cell fate decision between endoderm and mesoderm, demonstrating the similarity of BMP-induced hESC and EpiSC differentiation to mesoderm induction during mouse gastrulation. (qscience.com)
Differentiate human pluripotent stem cells (hPSCs) to cells of the endoderm lineage with our specialized cell culture media. (stemcell.com)
See More Use our definitive endoderm (DE) differentiation kit to obtain functional DE cells that are capable of downstream differentiation to hepatic, intestinal, pancreatic and pulmonary cells. (stemcell.com)
The cells were differentiated into definitive endoderm by using IDE1(induced definitive endoderm). (ac.ir)
In the present study, cells morphology and cells viability were evaluated by using a scanning electron microscopy and MTT assay respectively.The results of this study demonstrated the positive effect of 3D cultures, by using suitable factors, on definitive endoderm differentiation.definitive endoderm by using IDE1(induced definitive endoderm). (ac.ir)

Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem cells (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. (unlp.edu.ar)

Human embryonic stem cells (hESCs) derived from an early embryo can self-renew indefinitely and differentiate into a variety of cell types. (ashpublications.org)
To bridge this gap, we used three model cell types to study miRNA expression patterns: human embryonic stem cells (hESCs), hESCs-derived self-renewing neural stem cells (NSCs), and differentiating NSCs. (muni.cz)
Our data also indicates that miRNA clusters enriched in NSCs share the target-determining seed sequence with cell cycle regulatory miRNAs expressed in pluripotent hESCs. (muni.cz)

Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)

Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)
Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)
Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
The process of generating thrombocytes (BLOOD PLATELETS) from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via the MEGAKARYOCYTES. (bvsalud.org)

Exogenous IL-17a did not induce abnormal proliferation and migration of ASD and control NPCs but, on the other hand, it significantly increased the expression of synaptic (Synaptophysin-1, Synapsin-1) and neuronal polarity (MAP2) proteins in these cells. (figshare.com)
Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)

Cell transplantation studies were performed in mice with drug-induced acute liver failure to elicit benefits in hepatic support and tissue regeneration. (elsevierpure.com)
However, the scarcity of material sources greatly limits the application of primary pericytes in disease modeling and cell transplantation studies. (researchsquare.com)

It is known that less-differentiated and central-memory-like (termed highly reactive) CTLs are the optimal population for ACT-based immunotherapy, because these CTLs have a high proliferative potential, are less prone to apoptosis than more differentiated cells and have a higher ability to respond to homeostatic cytokines 2-7 . (psu.edu)

Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
Systematic AS characterization in single cells redefines our understanding of AS complexity in cell biology. (nih.gov)
This invention will significantly improve quality assurance, characterization, and application of pluripotent stem cells in both pre-clinical and clinical settings. (nih.gov)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)

Lactate may be applied to produce hepatocytes from iPS cells more efficiently. (spandidos-publications.com)
Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
These methods have been previously used to obtain hepatocytes from iPS cells. (spandidos-publications.com)
Therefore, methods for obtaining hepatocytes from iPS cells should be further investigated. (spandidos-publications.com)
A Hepatocyte selection medium (HSM) was developed to enrich hepatocytes from co-cultures with iPS cells ( 18 ). (spandidos-publications.com)
Gene expression profiling and comparison of regulatory networks in immature and mature hepatocytes revealed stem cell-derived hepatocytes represented early fetal-like stage. (elsevierpure.com)
The extent of metabolic and synthetic functions in stem cell-derived hepatocytes was sufficient for providing hepatic support along with promotion of tissue repair to rescue mice in acute liver failure. (elsevierpure.com)
In this study, we examined whether human iPS cells, cultured on scaffolds, can differentiate into definitive endodermal cells, as precursor for hepatocytes, pancreatic and lung cells. (ac.ir)

During liver development, cytokines released from various cell types are critical for stem/progenitor cell differentiation and lineage expansions. (elsevierpure.com)

Metabolic properties were analyzed to determine the extent of hepatic differentiation. (elsevierpure.com)
These studies showed signals emanating from fetal liver cells induced hepatic differentiation in stem cells. (elsevierpure.com)
We concluded that hepatic differentiation of pluripotent stem cells with metabolomics products will be significant for developing therapies. (elsevierpure.com)
Bandi, S , Tchaikovskaya, T & Gupta, S 2019, ' Hepatic differentiation of human pluripotent stem cells by developmental stage-related metabolomics products ', Differentiation , vol. 105, pp. 54-70. (elsevierpure.com)

Thus, induced pluripotent stem cells represent a promising combination of adult and embryonic stem cell characteristics. (prnewswire.com)
We explore the method on three circuits for haematopoiesis and embryonic stem cell development for commitment and reprogramming scenarios and illustrate how the method can be used to determine sequential steps for onsets of external factors, essential for efficient reprogramming. (lu.se)

CTLs can recognize malignant cells by interacting tumor antigens with the T cell receptors (TCR), and release cytotoxins as well as cytokines to kill malignant cells. (psu.edu)
TH2 cells produce cytokines that will trigger certain B cells. (wikipedia.org)
TH1 cells are created to make cytokines, like interferon gamma, that activate macrophages and cytotoxic T lymphocytes (CTLs). (wikipedia.org)
Many cytokines are pluripotent, in that each of these compounds can activate specific behavior in some cell types and inhibit other behavior in other cell types. (wikipedia.org)

Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for MSCs in future cell-free-based therapeutic approaches. (unlp.edu.ar)

However, when the same hiPS cells were differentiated in parallel, we did not observe high transcriptional similarity across differentiations. (figshare.com)
This project seeks to elucidate transcriptional and post-transcriptional regulatory networks that determine cell fate and guide the development of photoreceptors from retinal progenitor cells. (nih.gov)

In the Kirkeby lab, we further apply advanced human stem cells models and single-cell RNA sequencing to understand how the hundreds of human neural subtypes of cells are formed during embryo development. (lu.se)

Below, we outline a method that is based on published protocols as well as our own experience by which one can differentiate hPSCs along the pancreatic lineage to generate insulin-producing beta-cell-like cells. (ca.gov)
Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
Cells were grown in a directed, stepwise manner following established protocols. (indianactsi.org)
Australia allowed the other download Human Embryonic and Induced Pluripotent Stem Cells: Lineage Specific Differentiation Protocols to impose software for methods in 1902 and Canada was also system centuries as emotional Goodreads by video until 1922. (idealnaja.pl)
Understanding how embryonic cells biomolecularly restrict their early developmental potential and promote specific cell commitment helps to provide key insights on cancer plasticity and could improve fertilization protocols. (zeiss.com)

Endogenous cell signals regulate tissue homeostasis and are significant for directing the fate of stem cells. (elsevierpure.com)
This series covers biophysical regulation of stem cell fate and function in the context of tissue development. (biomedcentral.com)
In quick succession, the preimplantational mouse embryo undergoes a series of cellular divisions involving two critical cell fate decisions. (zeiss.com)
We previously discovered that that Maf-family bZIP transcription factor NRL is critical for rod photoreceptor fate and functional differentiation, and that loss of NRL leads to S-cones instead of rods. (nih.gov)

For example, retinal cells that could be used to replace damaged retinal tissue. (jove.com)
The differentiation mechanisms involving metabolomics products could have an impact on advancing recruitment of stem/progenitor cells during tissue homeostasis. (elsevierpure.com)
Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
Advances in the fields of stem cell biology, biomaterials, and tissue engineering over the last decades have brought the possibility of constructing tissue substitutes with a broad range of applications in reg. (biomedcentral.com)
The possibilities arising from these characteristics have resulted in great commercial interest, with potential applications ranging from the use of stem cells in reversal and treatment of disease, to targeted cell therapy, tissue regeneration, pharmacological testing on cell-specific tissues, and more. (prnewswire.com)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
To model complex human brain diseases in the dish using stem cell-derived brain tissue models. (lu.se)

These cells can differentiate into many subtypes once activated by antigen presenting cells (APCs) like dendrites. (wikipedia.org)

Pluripotent is also described as something that has no fixed developmental potential, as in being able to differentiate into different cell types in the case of pluripotent stem cells. (wikipedia.org)

Cells were initially edited by the CRISPR/Cas9 system to express the Thy1 gene at the Brn3b locus and the tdTomato gene at the melanopsin locus. (indianactsi.org)
Bimodal exons are flanked by more conserved intronic sequences harboring distinct cis-regulatory motifs, constitute much of cell-type-specific splicing, are highly dynamic during cellular transitions, preserve reading frame, and reveal intricacy of cell states invisible to conventional gene expression analysis. (nih.gov)
generation and targeted differentiation of induced pluripotent stem cells) to identify novel gene defects. (nih.gov)
Using this approach, the laboratory has identified several new gene defects, including DOCK2 deficiency, TTC7A deficiency (responsible for combined immune deficiency and intestinal atresias), HOIP deficiency (a cause of immune deficiency and autoinflammatory disease), EXTL3 deficiency (associating severe T-cell immunodeficiency, skeletal dysplasia, and neurodevelopmental delay), PAX1 deficiency and SASH3 deficiency. (nih.gov)
We are using state-of-the-art next generation sequencing combined with bioinformatic strategies, and developing stem cell-based approaches for gene therapy and drug discovery. (nih.gov)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)

therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. (biomedcentral.com)
Consequently, because of the increased rate of RF in terminal stage and few alternative treatments, new investigations focused on regenerative medicine have been developed, including the use of stem cells (SCs) [ 7 ]. (biomedcentral.com)
Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)

To determine mechanisms in these stage-specific lineage interactions, we modeled potential effects of soluble signals derived from immortalized human fetal liver parenchymal cells on stem cells, including embryonic and induced pluripotent stem cells. (elsevierpure.com)
Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

The successful differentiation of brain pericyte-like cells from human pluripotent stem cells may allow us to study their biological characteristics and their applications in the treatment of pericyte dysfunction-related neurodegenerative diseases, including ischemic stroke, Parkinson's disease (PD) and so on. (researchsquare.com)
Conditions such as Huntington's disease, Parkinson's disease, and spinal cord injuries are examples of clinical applications in which stem cells could offer benefits in halting or even reversing damage. (prnewswire.com)
Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)
In particular, we aim to enter a clinical trial with a pluripotent cell product for the treatment of Parkinson's Disease, and are also developing preclinical cell therapies for narcolepsy and dementia. (lu.se)
To enter a clinical trial with a stem cell product for the treatment of Parkinson's Disease. (lu.se)

Embryonic or adult SCs by definition are characterized by their self-renewal and potentiality, peculiarities that allow them to give rise to more SCs and differentiate into various cell lineages under appropriate conditions. (biomedcentral.com)
The involvement of microRNAs (miRNAs) in orchestrating self-renewal and differentiation of stem cells has been revealed in a number of recent studies. (muni.cz)
Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)

Our research focuses on understanding how pluripotent mammalian stem cells maintain their undifferentiated state and undergo differentiation in culture - this reflecting my enduring interest in the emergence of diversity during mouse gastrulation. (qscience.com)
Stem cells reside within most tissues throughout the lifetimes of mammalian organisms. (biomedcentral.com)

Skeletal muscle differentiation is triggered by a unique family of myogenic basic helix-loop-helix transcription factors, including MyoD, MRF-4, Myf-5, and Myogenin. (unipv.it)
We investigate the role of Zeb2 in skeletal muscle differentiation using genetic tools and transgenic mouse embryonic stem cells, together with single-cell RNA-sequencing and in vivo muscle engraftment capability. (unipv.it)

However, scientists have found a way to reprogram these mature cells so that they "de-differentiate" and return to an unspecialized, proliferative state. (jove.com)
The transcription factors then turn on many other genes that are expressed by embryonic stem cells, returning the cells to an undifferentiated, proliferative, and pluripotent state. (jove.com)

Next, we compared both datasets and found that while iPSC EVs enclose proteins that modulate RNA and microRNA stability and protein sorting, PD-MSC EVs are rich in proteins that organize extracellular matrix, regulate locomotion, and influence cell-substrate adhesion. (unlp.edu.ar)
The ability to understand and regulate human vasculature development and differentiation has the potential to benefit patients suffering from a variety of ailments, including cardiovascular disease, peripheral. (biomedcentral.com)

doi: 10.1016/j.stem.2020.07.022. (nih.gov)

However, due to difficulties in obtaining a high number of such CTLs from patients, there is an urgent need to find a new approach to generate highly reactive Ag-specific CTLs for successful ACT-based therapies.TCR transduction of the self-renewable stem cells for immune reconstitution has a therapeutic potential for the treatment of diseases 8-10 . (psu.edu)
To develop novel cell therapies for neurodegenerative diseases. (lu.se)
Such stem cell therapies have the potential to repair the damaged brain, making patients independent of daily medications with severe side effects. (lu.se)
Through our work on developing novel stem cell therapies for brain diseases, we contribute to the main goal of MultiPark on establishing novel therapeutic approaches for developing more effective symptomatic, new disease-modifying, and/or plasticity-enhancing treatments. (lu.se)

The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
Traditionally, scientists have worked with both embryonic and adult stem cells for research tools, as well as for cellular therapy. (prnewswire.com)
Although some adult stem cells do have differentiation capacity, it is often limited in nature, which results in fewer options for use. (prnewswire.com)

In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. (biomedcentral.com)

The protocol we present in the study provides a cranial neural crest originated, fast and robust forebrain pericyte-like cells differentiation method using either human embryonic stem cells or human induced pluripotent stem cells as a starting material. (researchsquare.com)

Taken together, these studies should significantly accelerate the progression from basic stem cell research to clinical applications. (qscience.com)
Here, we found that GMPPB is highly expressed in a panel of GBM cell lines and clinical samples and that expression of GMPPB is positively correlated with the WHO grade of gliomas. (bvsalud.org)
A second press release [2] has indicated stem cell source, resulting in widespread excitement that the program is planning to move forward very rapidly, with all of the patients being enrolled in the Correspondence to: Roger A. Barker, John van Geest Centre first quarter of 2016 and interim results being shared for Brain Repair, Department of Clinical Neurosciences, Univer- in October 2016. (lu.se)

Then, genes for multiple transcription factors are delivered into the cells using a viral vector, and the transcription factor proteins are expressed using the cell's machinery. (jove.com)
We then carried out a detailed analysis of the roles of Smad proteins (Smad2 and Smad3) as direct regulators of Nanog, which in turn blocks an hESC default differentiation into neuroectoderm. (qscience.com)
b Cells were collected and heated in a narrow temperature range, with one sample incubated at 37 °C for expression measurements, protein aggregates were eliminated by ultracentrifugation and the soluble proteins were digested. (nature.com)
Unexpectedly, differentiation-inducing soluble signals constituted metabolomics products and not proteins. (elsevierpure.com)

Induced pluripotent stem cells represent one of the most promising research advances within the past decade, making this a valuable report for both executives and investors to use to optimally position themselves to sell iPSC products. (prnewswire.com)

By immunostaining for cell type specific markers, I have documented the different stages of retinal differentiation and provided insight into the mechanisms of ipRGC differentiation. (indianactsi.org)
The answers to these questions will be valuable for delineating pathogenic mechanisms that contribute to photoreceptor cell death. (nih.gov)

Anatomy66

Organisms12

Diseases5

Chemicals and Drugs68

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes60

Disciplines and Occupations5

Technology, Industry, Agriculture5

Information Science3

Health Care1

Mesenchymal stem3

Differentiated from human pluripotent stem1

IPSCs15

Neuronal differentiation4

Maturation6

Type of pluripotent2

Human induced plur2

Abstract2

Cardiac1

Hematopoietic stem2

Organoids3

Progenitors3

Growth and differentiation2

Pluripotency8

Neurons6

ESCs9

Vitro17

Pathways6

Osteogenic1

Lineage differentiation1

Endoderm5

Stromal cells1

HESCs3

Molecular Biology1

Bone marrow5

Proliferation2

Transplantation studies2

Apoptosis1

Characterization4

Hepatocytes8

Progenitor cell1

Hepatic4

Embryonic stem2

Cytokines4

Vesicles1

Transcriptional2

Kirkeby1

Protocols5

Fate4

Tissue7

Subtypes1

Developmental1

Gene6

Regenerative4

Fetal3

Parkinson's8

Self-renewal3

Mammalian2

Skeletal2

Proliferative2

Regulate2

10.10161

Therapies4

Adult stem3

Spectroscopic1

Protocol1

Clinical3

Proteins4

Advances1

Mechanisms2