Pluripotent stem endothelial stem cells. Medical search. Frequent questions

The components of STEMdiff™ Endothelial Expansion Culture Kit are included in STEMdiff™ Endothelial Differentiation Kit (Catalog #08005). (stemcell.com)
Using appropriate media conditions for differentiation of human pluripotent cells to CD34+ vascular progenitor cells, we show that temporary expression of pluripotent transcription factors and treatment with chemically-defined media, will induce differentiation of human fibroblasts to CD34+ vascular progenitor cells. (nih.gov)
Scholars@Duke publication: Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress. (duke.edu)
The recently disclosed plasticity properties of adult-derived stem cells, their ability to be reprogrammed by defined factors into pluripotent stem cells and the comprehension of "epi"-genetic mechanisms underlying stem cells differentiation process has opened unexpected avenues to attempt regeneration of tissues affected by degenerative disorders and prompted the birth of the new "regenerative medicine" concept. (mpi.nl)
Dorsomorphin dihydrochloride also inhibits bone morphogenetic protein (BMP) type I receptors (ALK2, ALK3 and ALK6), promotes cardiomyogenesis in mouse embryonic stem cells (ESCs) in vitro and promotes neural differentiation of hPSCs as part of a chemical cocktail. (tocris.com)
Remaining obstacles include the high expense of differentiation media, use of animal-derived materials and the need for cell sorting [ 13 , 14 , 15 ]. (biomedcentral.com)
In vivo and in vitro experiments demonstrate that not only vascular endothelial cells growth factor (VEGF) enhances EC fate commitment, but also basic fibroblast growth factor (bFGF) further facilitates EC differentiation [ 17 , 18 , 19 ]. (biomedcentral.com)
Variation of DNA methylation on the IRX1/2 genes is responsible for the neural differentiation propensity in human induced pluripotent stem cells. (reprocell.com)
Pancreatic Differentiation of Stem Cells Reveals Pathogenesis of a Syndrome of Ketosis-Prone Diabetes. (reprocell.com)
Biomaterials USA manufactures 2D and 3D structures for robust cell growth and differentiation. (biomaterialsusa.com)
Kogut I, Roop DR, Bilousova G. Differentiation of human induced pluripotent stem cells into a keratinocyte lineage. (ucdenver.edu)
Using ATOs developed from different cell lines the trainee assessed the ability to support CD34+ differentiation. (ca.gov)
The FUNGENES project aimed to achieve a detailed basic understanding of stem cell self-renewal and differentiation. (europa.eu)
FUNGENES identified the gene subsets that are active in self-renewing cells, during the exit from pluripotency and at specific stages of mouse ES cell differentiation. (europa.eu)
Understanding the genetic pathways underlying differentiation of ES cells to somatic cells would contribute to future therapeutic strategies for degenerative diseases such as heart disease, diabetes and Parkinson's. (europa.eu)
We use our simplified differentiation platform and single-cell RNA-sequencing to achieve further insights into PGCLC specification. (bvsalud.org)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Stem cells can divide into two cells identical to the original stem cell or into differentiated cell types, depending on their origin and differentiation potency. (bvsalud.org)
By definition, adult stem cells are capable of differentiation into at least two lineages and have the property of self-renewal. (bvsalud.org)
The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)

Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most. (koreamed.org)
The work is an important step with fragile endothelial cells (ECs) made from "induced pluripotent stem cells," or iPSCs, a type of cell that can potentially be made from the cells of any human patient. (sciencedaily.com)
Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. (duke.edu)
Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. (duke.edu)
The compound can also be used in protocols for the chemical reprogramming of somatic cells to iPSCs.Shown to induce autophagy in cancer cell lines via a mechanism independent of AMPK inhibition. (tocris.com)
They are also a suitable cellular substrate for the generation of induced pluripotent stem cells (iPSCs) via nuclear reprogramming, offering a number of advantages over other cell types. (uea.ac.uk)
Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. (stanford.edu)
Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. (stanford.edu)
In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research. (stanford.edu)
This research aimed to isolate human umbilical wire blood derived endothelial colony forming cells (ECFCs) adopted by their integration free reprogramming in direction of induced pluripotent stem cells (iPSCs) and molecular characterization of each cell sorts utilizing multicolour flowcytometery and immunofluorescence respectively. (heraeus-targets.com)
The ECFCs remoted after ficoll primarily based separation of wire blood mononuclear cells (CBMNCs) which on emergence characterised by way of move cytometry and reprogrammed in direction of induced pluripotent stem cells (iPSCs) utilizing episomal vectors, the iPSCs had been characterised utilizing immunofluorescence. (heraeus-targets.com)
Our final student worked to introduce a "suicide gene" construct into engineered in iPSCs for the generation of the insulin-producing -cell to treat type 1 diabetes patients. (ca.gov)

This pure synthetic product can be used to replace matrigel, laminin, collagen, or fibronectin in supporting human endothelial progenitor cell cultures. (biomaterialsusa.com)
We searched the databases such as EMBASE, PubMed, and Web of Science with the keywords "stem cell," "progenitor cell," "satellite," "senescence" and excluded the keywords "cancer," "tumor," "malignancy" and "carcinoma" until June 2020. (magiran.com)

Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
A second trainee focused on developing a fluorescent marker to allow for the characterization of a CAR-T cell population as it interacts with the targeted brain tumor. (ca.gov)

Functional studies revealed increased expression of ICAM-1 on induced patient-derived endothelial cells (iECs) and of β2-integrins on patient neutrophils that increase neutrophil adhesion and vascular transendothelial migration (TEM). (nature.com)
However, the recent identification of blood outgrowth endothelial cells (BOECs), generated from circulating endothelial progenitors in adult peripheral blood, may circumvent this limitation by offering an endotheliallike, primary cell surrogate for patient-derived endothelial cells. (uea.ac.uk)

Endothelial colony forming cells (or ECFCs) are adult endothelial progenitor cells capable of differentiating to regenerate endothelial cell populations. (wikipedia.org)
Certain cells within the heterogeneous group of colony forming cells are demonstrated to reach significantly higher population doublings, and retain high levels of telomerase activity. (wikipedia.org)
These have been termed high proliferative potential endothelial colony forming cells, or HPP-ECFCs. (wikipedia.org)
Vascular endothelial stem cells have been defined as rare endothelial colony forming cells with extremely high proliferative potential. (wikipedia.org)
To the perfect of our information this research was the primary try and integration free reprogramming of wire blood derived endothelial colony forming cells in direction of induced pluripotent stem utilizing episomal plasmids. (heraeus-targets.com)

Collink.3D is compatible with major 3D bioprinting technologies and cell types including stem cells, induced pluripotent stem cells, endothelial, and epithelial cells. (prnewswire.com)
One trainee focused on developing in vitro growth conditions to optimize expansion of luminal epithelial cells and c-KIT+ progenitor cells to explore the early stages of breast cancer. (ca.gov)
Finally, we show that spironolactone exerts a dose-dependent inhibitory effect on viral entry in human lung epithelial cells. (stanford.edu)

It thoroughly investigated the unique ability of mouse embryonic stem cells to develop into cells of any organ ("pluripotency"), created new tools for functional genomic studies and thus provided key knowledge to understanding the commitment of cells to differentiate into cells of a particular germ layer. (europa.eu)
Embryonic stem cells are derived from the inner cell mass of a blastocyst, which is an accumulation of primordial cells (4-5 days after fertilization). (bvsalud.org)

In vascular medicine, various stem cells and adult progenitors have been highlighted as having a vasoreparative role in ischaemic tissues. (biomedcentral.com)
What is a unified definition for endothelial progenitors or angioblasts, and when do arterial vs. venous potentials segregate? (bvsalud.org)

In a paper published online in the journal Biomaterials Science , a team from the laboratories of Rice bioengineer Jordan Miller and Baylor College of Medicine biophysicist Mary Dickinson showed how to use a combination of human endothelial cells and mesenchymal stem cells to initiate a process called tubulogenesis that is crucial to the formation of blood-transporting capillaries. (sciencedaily.com)
The clinical-grade cell line RC11 was used to generate hESC-ECP which were identified as mostly endothelial (60% CD31+/CD144+), with the remainder of the subset expressing various pericyte/mesenchymal stem cell markers. (ed.ac.uk)
Comparative analysis of human induced pluripotent stem cell-derived mesenchymal stem cells and umbilical cord mesenchymal stem cells. (reprocell.com)
Our investigation indicates that most of these studies examined endothelial progenitor cells, hematopoietic stem cells, mesenchymal stem cells, adipose-derived stem cells, and a few others were about less-discussed types of stem cells such as cardiac stem cells, myeloblasts, and induced pluripotent stem cells. (magiran.com)
McGarvey SS, Ferreyros M, Kogut I, Bilousova G. Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells. (ucdenver.edu)

In particular, FUNGENES identified genes controlling the development of pluripotent embryonic stem (ES) cells into heart cells (cardiomyocytes), nerve cells (neurons), smooth muscle cells, vascular endothelial cells, fat cells (adipocytes), liver cells (hepatocytes) and insulin-producing cells of the pancreas. (europa.eu)

ECFCs are named for their ability to form colonies of cells which progress rapidly to capillary-like networks in vitro when cultured in biopolymer matrix, and in vivo. (wikipedia.org)
We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques. (stemcell.com)
Stem cell senescence in the in vitro expansion stage is an essential concern in clinical procedures of cell therapy. (magiran.com)
Owing to their manifold roles in health and disease, there have been intense efforts to synthetically generate blood vessels in vitro from human pluripotent stem cells (hPSCs). (bvsalud.org)
How can we specifically generate either arterial or venous endothelial cells (ECs) from hPSCs in vitro? (bvsalud.org)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)

Mesodermal progenitor cells are further differentiated into ECs by utilizing a combination of human vascular endothelial growth factor A165 (VEGF), basic fibroblast growth factor (bFGF), 8-Bromoadenosine 3′,5′-cyclic monophosphate sodium salt monohydrate (8Bro) and melatonin (Mel) for 48 h. (biomedcentral.com)

BACKGROUND: Autologous stem cell transplantation (autoSCT) can extend remission of mantle cell lymphoma (MCL), but the management of subsequent relapse is challenging. (koreamed.org)
BACKGROUND: This study aimed to assess the outcome of stem cell transplantation (SCT), including overall survival (OS), failure-free survival (FFS) and graft-versus-host disease (GvHD)-free/failure-free survival (GFFS), and to analyze prognostic. (koreamed.org)
Beyond their value to understanding endothelial biology and disease modeling, BOECs have potential uses in endothelial cell transplantation therapies. (uea.ac.uk)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases (such as Krabbe disease), including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses as well as peroxisome disorders such as X-linked adrenoleukodystrophy. (medscape.com)
Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)
Some evidence indicates that at least in certain disorders, combination ERT and hematopoietic stem cell transplantation together might be superior to hematopoietic stem cell transplantation alone in patients who are appropriate candidates. (medscape.com)
The availability of both ERT and hematopoietic stem cell transplantation has prompted ongoing consideration of newborn screening efforts to diagnose lysosomal storage diseases. (medscape.com)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases, including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses. (medscape.com)

These cells are classified as totipotent and they can form any of the tissue types found in the adult body, in addition to having unlimited proliferation potential 6 . (bvsalud.org)

While tissue engineers have found dozens of ways to coax stems cells into forming specific kinds of cells and tissues, they still cannot grow tissues with vasculature -- capillaries and the larger blood vessels that can supply the tissues with life-giving blood. (sciencedaily.com)
Each of the trillions of living cells in the human body are constantly supplied with oxygen and nutrients by tiny blood vessels known as capillaries. (sciencedaily.com)
Measuring just a few thousandths of a millimeter in diameter, some capillaries are so narrow that individual blood cells must squeeze through them in single-file. (sciencedaily.com)
Capillaries are made entirely from networks of endothelial cells, the type of cell that lines the inner surface of every blood vessel in the human body. (sciencedaily.com)
In the process of tubulogenesis -- the first step to making capillaries -- endothelial cells undergo a series of changes. (sciencedaily.com)
First, they form small, empty chambers called vacuoles, and then they connect with neighboring cells, linking the vacuoles together to form endothelial-lined tubes that can eventually become capillaries. (sciencedaily.com)
Brain microvascular endothelial cells (BMECs) line the capillaries, forming the BBB. (vanderbilt.edu)

Tissue engineering, also known as regenerative medicine, is a field aimed at integrating advances in stem cell biology and materials science to grow transplantable replacement tissues and organs. (sciencedaily.com)
Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)
The development of transgenic mouse models that express genes of interest in specific cell types has transformed our understanding of basic biology and disease. (stanford.edu)
His Nitric oxide study incorporates themes from Anesthesia, Gene isoform, Pharmacology and Cell biology. (research.com)
His Nitric oxide study incorporates themes from Pharmacology, Erg, Cell biology, Reperfusion injury and Cerebral blood flow. (research.com)
Development: For advances in developmental biology and stem cells. (lu.se)

Pluripotent stem cell-derived differentiated endothelial cells offer high potential in regenerative medicine in the cardiovascular system. (ed.ac.uk)
City of Hope's Train, Educate and Accelerate Mastery of Stem cell research (TEAMS) program was built around four key areas of regenerative medicine research: Cancer Stem Cells, Hematopoietic Stem Cells, Tissue-Specific Stem Cells and Human Pluripotent Stem Cells. (ca.gov)
The use of cell therapy for vascular regeneration offers an exciting new prospect in regenerative medicine. (biomedcentral.com)

Ubiquitination and autophagy target proteins via post-translational modifications for regulating the protein homeostasis of amyloid precursor protein (APP) and its harmful byproduct amyloid beta (Aβ) in neurons, but also in other cells of the neurovascular unit including the BBB. (uniklinikum-jena.de)
We study ubiquitination and autophagy in induced pluripotent stem cell-derived brain-capillary endothelial cells, pericytes, astrocytes, microglia, and neurons. (uniklinikum-jena.de)
Autophagy is a critical process, compromised in neurodegenerative disease, by which terminally differentiated cells like neurons manage cytoskeletal and organelle turnover. (omeka.net)

They have been identified by marker analysis as lin- (lineage negative) CD31+, CD105+, Sca-1+, CD117 (ckit)+ and thought have the ability to generate functional vasculature from single cells. (wikipedia.org)
After 7 days of mesoderm and endothelial induction, the resulting cells can be expanded for 5 - 6 passages to generate large numbers of highly pure, mature endothelial cells that typically reach ≥ 99% CD144+CD31+ by passage 2 and maintain this phenotype until passage 5. (stemcell.com)
Using Microfluidics to Generate Human Naïve and Primed Pluripotent Stem Cells. (reprocell.com)
In 2017, the Thomson Lab was able to generate and characterize endothelial cells. (universityresearchpark.org)
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. (stemcell.com)
Stem cell research endeavors to generate specific subtypes of classically defined "cell types. (bvsalud.org)
Here, we generate >90% pure human artery or vein endothelial cells from pluripotent stem cells within 3-4 days. (bvsalud.org)

At the Morgridge Institute, stem cell pioneer James Thomson is leading a potentially transformational project to develop a safe and functional cell-based artificial artery that could be pulled from medical inventories and used by vascular surgeons. (universityresearchpark.org)
Generating human artery and vein cells from pluripotent stem cells highlights the arterial tropism of Nipah and Hendra viruses. (stanford.edu)
We specified artery cells by inhibiting vein-specifying signals and vice versa. (bvsalud.org)

Thus, we will address the question, whether in our induced pluripotent stem cell-derived BBB models these processes are affected by age and/or genetic preposition that are associated with Alzheimer's disease. (uniklinikum-jena.de)

This review will examine the clinical potential of several stem and progenitor cells that may be utilised to regenerate defunct or damaged vasculature and restore blood flow to the ischaemic tissue. (biomedcentral.com)

Submitter supplied) We report a novel technique to reprogram human fibroblasts into endothelial and smooth muscle cells using partial iPSC reprogramming and chemically defined media. (nih.gov)
We characterize the role of increased Lyn kinase activity in neutrophils, endothelial cells and lesional liver biopsies and utilized an iPSC-derived endothelial cell platform for disease modeling of neutrophilic vasculitis and to screen and evaluate drug efficacy. (nature.com)
Chad and I have both been principal investigators on the induced pluripotent stem cell (IPSC) grant. (medscape.com)

Bone marrow appears to contain three stem cell populations: hematopoietic stem cells, stromal stem cells and endothelial precursor cells. (bvsalud.org)

Single transcription factor efficiently leads human induced pluripotent stem cells to functional microglia. (reprocell.com)
All these diseases are characterised by the irreversible loss of functional cells. (europa.eu)
Cells for vascular therapy must be able to home to ischaemic or damaged tissue and engage in vessel formation alone or in unison with resident vasculature to achieve a controlled and functional reperfusion event, without causing pathological angiogenesis (for example, proliferative retinopathy in the vitreous of the eye). (biomedcentral.com)
Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)

Three pulmonary veno-occlusive disease patients who carried the compound heterozygous variants of EIF2AK4 and two healthy controls were recruited and induced pluripotent stem cells were generated from human peripheral blood mononuclear cells. (stemcellsciencenews.com)

A second trainee examined the role of Sae2 in DNA replication, a critical step in growth and self-renewal of pluripotent stem cells. (ca.gov)
A stem cell is an undifferentiated unit with powerful self-renewal properties that is capable of organizing other cell types in the body. (bvsalud.org)
Stem cells are defined as undifferentiated cells that have precursor properties, are capable of forming many different cell types and have the property of unlimited self-renewal 5 . (bvsalud.org)

Historically, the limited availability of primary endothelial cells from patients with vascular disorders has hindered the study of the molecular mechanisms underlying endothelial dysfunction in these individuals. (uea.ac.uk)
A third trainee looked at the relationship between -cell and endothelial cell dysfunction and the role of endothelial Argonaute 1(AGO1) in this process. (ca.gov)

Moreover, the concepts discussed here are readily adapted to other genomic loci to accurately measure mixed cell populations in any tissue. (stanford.edu)
(A) Lamina-associated domains (LADs), at the nuclear envelope, schematized from analyses of cell populations using genome-wide approaches such as DamID- or ChIP-sequencing. (frontiersin.org)
(B) Analysis of single cells however (e.g., by FISH) indicates that not all LADs mapped in cell populations are found at the nuclear periphery in all cells. (frontiersin.org)

Finally, hPSC-derived PGCLCs can be easily purified by virtue of their CXCR4+PDGFRA-GARP- surface-marker profile and single-cell RNA-sequencing reveals that they harbor transcriptional similarities with fetal PGCs. (bvsalud.org)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

The present review will cover light and shaded areas in the cardiovascular cellular therapy field, and will discuss about recent advances and related patents designed to enhance efficiency of stem cell therapy in patients with cardiovascular disease. (mpi.nl)

In addition to the aging phenomenon, stem cell senescence has been investigated in various concepts such as cancer, adverse drug effects, and as a limiting factor in cell therapy. (magiran.com)
A cell therapy approach should be aimed at promoting revascularisation of ischaemic tissue. (biomedcentral.com)
Finally, one should consider cell choice, a critical aspect of any cell therapy. (biomedcentral.com)

McGrath PS, McGarvey SS, Kogut I, Bilousova G. Efficient RNA-Based Reprogramming of Disease-Associated Primary Human Fibroblasts into Induced Pluripotent Stem Cells. (ucdenver.edu)
The microenvironment, consisting of lymphocytes, macrophages, fibroblasts, endothelial elements, and stroma in which hematopoietic cells reside, creates a regulatory niche that determines the local area network. (medscape.com)

They are residents of adult vasculature and are also thought to migrate to areas of injury as one form of circulating endothelial cell. (wikipedia.org)
This approach (i) allows for the generation of patient-specific endothelial cells from a relatively small volume of adult peripheral blood and (ii) produces cells that are highly similar to primary endothelial cells in morphology, cell signaling and gene expression. (uea.ac.uk)
Organoids are grown either from pluripotent stem cells or from tissue-specific adult stem cells. (journalajpr.com)
Adult stem cells are present in a large number of tissues and are responsible for renewing the cells in these tissues. (journalajpr.com)
Many studies have shown the utility of embryonic or adult stem cells for forming teeth and for regeneration of bone and soft tissues. (bvsalud.org)
There are basically two types of stem cells: embryonic and somatic (or adult). (bvsalud.org)
Adult stem cells are classified as pluripotent and are undifferentiated cells that remain quiescent in tissues until stimulated, when they can create cell types that are compatible with the tissue in which they reside. (bvsalud.org)

Its major objective was to produce a gene expression atlas covering the development of ES cells into all three germ layers (ecto-, meso-, and endoderm) and into somatic cells. (europa.eu)
Thus, somatic cells (primitive streak) and PGCLCs are related-yet distinct-lineages segregated by temporally-dynamic signaling. (bvsalud.org)

Stem cells are thereby induced to differentiate by molecular signals and grown in culture systems that promote their three-dimensional self-organization. (journalajpr.com)

Our third trainee in this group focused on the generation of human artificial thymic organoids (ATO) from umbilical cord blood derived hematopoietic stem cells to allow for the study of T cell development. (ca.gov)

In fact, they unpredictably indicated modest, yet significant, clinical benefits in patients compared to the outstanding results using stem cells in animal models of ischemic heart and peripheral disease. (mpi.nl)

Generating primordial germ cell-like cells (PGCLCs) from human pluripotent stem cells (hPSCs) advances studies of human reproduction and development of infertility treatments, but often entails complex 3D aggregates. (bvsalud.org)

My research is focused on understanding what stem cell secreted factors are responsible for improved heart function, what their targets are and how these can be modulated to develop new cell-free therapies that can help patients with a wide spectrum of coronary disease and heart failure. (buffalo.edu)

Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
The key to these cells' utility is their ability to differentiate into many different cell types depending on the stimulus received and they have been used in treatments for diseases such as cancer and neural degeneration, in rehabilitation of tetraplegic and paraplegic patients and even in dentistry 1 . (bvsalud.org)
Hematopoietic stem cells are cells isolated from blood and bone marrow that can differentiate into a variety of different specialized cells and suffer apoptosis 1 . (bvsalud.org)

Dissecting cell identity via network inference and in silico gene perturbation. (nih.gov)
Patient-derived induced pluripotent stem cells were derived into neural precursor and endothelial cells to study gene expression. (cu.edu.eg)
This analysis led to the discovery of a population of preadipocyte cells whose gene expression is remodeled in an age-specific fashion. (ca.gov)
Specifically, the trainee looked at the ability of an endothelial cell AGO 1 gene knockout to rescue streptozotocin-induced damage in female mice. (ca.gov)
Understanding the activity of this gene will allow for an improved understanding of how cells can survive DNA damage leading to genomic rearrangements that support the initial stages of tumorgenesis and subsequent cancer development. (ca.gov)

EMBO Journal] The authors provide an in-depth review of the metabolic and epigenetic interplay regulating hematopoietic stem cell fate, and discuss the influence of metabolic stress stimuli, as well as alterations occurring during leukemic development. (stemcellsciencenews.com)

We're thrilled to have Chad Cowan, an associate professor at Harvard University who is at the Harvard Stem Cell Institute. (medscape.com)

Efficiently expand human pluripotent stem cell (hPSC)-derived endothelial cells using xeno-free STEMdiff™ Endothelial Expansion Culture Kit (Catalog #100-1218). (stemcell.com)

These cells showed lack of PCDH12 expression and impaired neurite outgrowth. (cu.edu.eg)

We established LOAD-specific induced pluripotent stem (iPS) cell models for microglia and BBB cultures. (uniklinikum-jena.de)

List of human cell types derived from the germ layers Mund JA, Estes ML, Yoder MC, Ingram DA, Case J (2012). (wikipedia.org)
Producing arteries in the lab requires two essential cell types: endothelial cells, which line the interior of blood vessels, and smooth muscle cells from pluripotent stem cells, Thomson says. (universityresearchpark.org)
We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. (nature.com)
a Cells of different types in were grown simultaneously ( n = 3 biologically independent samples per cell type). (nature.com)
However, the accurate quantitation of mixed cell types in chimeric and mosaic tissues is complicated by sample preparation bias, transgenic silencing, phenotypic similarity, and low-throughput analytical pipelines. (stanford.edu)
We demonstrate that transgenic AAVR overexpression greatly increases the efficiency of transduction of many diverse cell types, including muscle stem cells, which are normally refractory to AAV transduction. (stanford.edu)
Organoids are three-dimensional, organ-like cell assemblies in which different cell types have organized themselves in a way that is approximately typical for the corresponding organ in the body. (journalajpr.com)
They can only give rise to the cell types that are present in the particular tissue, the stem cell of the intestinal epithelium only produces cells of the intestinal epithelium, but not muscle cells or nerve cells. (journalajpr.com)
In any case, the respective cell types may then be selectively cultured to enrich their percentage population to eventually obtain a single cell type and culture. (justia.com)
Stem cells are nonspecific cells with powerful self-regeneration properties and they are capable of organizing other cell types in the body. (bvsalud.org)
Additionally, they can form specialized cell types from other tissues if they are transplanted 6 . (bvsalud.org)

Anatomy59

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