• To generate iPSCs, dermal fibroblasts were cultured from the skin biopsy sample and transduced according to previously described protocols 5 . (nature.com)
  • The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
  • Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. (elifesciences.org)
  • In this study, employing iPS technology, we derived and characterized iPSCs from dermal fibroblasts of an 82-year-old female patient affected by sporadic AD. (biomedcentral.com)
  • These properties can be illustrated with relative ease in vitro, using methods such as clonogenic assays, where the progeny of a single cell is characterized. (wikipedia.org)
  • However, it is known that in vitro cell culture conditions can alter the behavior of cells, proving that a particular subpopulation of cells possesses stem cell properties in vivo is challenging, and so considerable debate exists as to whether some proposed stem cell populations in the adult are indeed stem cells. (wikipedia.org)
  • It can be induced by modifying the growth medium when stem cells are cultured in vitro or by transplanting them to an organ of the body different from the one they were originally isolated from. (wikipedia.org)
  • Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
  • The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
  • Cell culture -Growth of cells in vitro on an artificial medium for experimental research. (cellmedicine.com)
  • Embryonic stem cell line -Embryonic stem cells, which have been cultured under in vitro conditions that allow proliferation without differentiation for months to years. (cellmedicine.com)
  • Some researchers are attempting to use stem cells to bioengineer human organs in the lab in vitro , rather than inside another species (5). (the-scientist.com)
  • Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
  • On the other hand, even if 3D aggregate culture methods are able to recapitulate in vitro the cell-extracellular matrix interactions properly observed in vivo, and the synthetic/natural matrix and scaffolds have biochemical and mechanical properties, in order to mimic the native extracellular matrix, both of these systems possesses some limitations and some methodological improvements are needed. (razavihospital.ir)
  • Results: The processes by which re-aggregated adult single cell types or adult and embryonic explanted tissues are able to recapitulate embryogenesis in vitro, when cultured in adhesion or embedded in 3D gels, is not surprising and is clearly under the control of a reminiscent cellular memory which recapitulates early developmental stages. (razavihospital.ir)
  • Moscona A. The Development in Vitro of Chimeric Aggregates of Dissociated Embryonic Chick and Mouse Cells. (razavihospital.ir)
  • Kurosawa H. Methods for inducing embryoid body formation: in vitro differentiation system of embryonic stem cells. (razavihospital.ir)
  • Culture and differentiation of preadipocytes in two-dimensional and three-dimensional in vitro systems. (razavihospital.ir)
  • For research purposes, embryonic stem cells from surplus, artificially inseminated embryos (in vitro fertilization) are currently being harvested in various countries, for example in Belgium and the United Kingdom. (understanding-stemcells.info)
  • Therefore, the implantation of human cells reprogrammed in vitro represents an alternative strategy [ 16 - 19 ]. (thno.org)
  • This suggests that the natural cardiac milieu may be a critical factor for direct cardiac reprogramming [ 2 , 14 , 22 , 23 ], and delivery of cues in the cardiac microenvironment to fibroblasts may improve the efficiency of direct cardiac reprogramming in vitro . (thno.org)
  • The neuronal cells expressed numerous genes associated with sub-regions within the brain thus suggesting the usefulness of our in-vitro model. (biomedcentral.com)
  • Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)
  • As a result of over five decades of investigation, mesenchymal stromal/stem cells (MSCs) have emerged as a versatile and frequently utilized cell source in the fields of … MSC have been shown to differ-entiate in vitro into adipocytes, chon-drocytes, osteoblasts, myocytes, and ß-pancreatic islets cells. (tomeipowered.com)
  • The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. (tomeipowered.com)
  • 2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
  • Sääf AM, Halbleib JM, Chen X, Tsan Yuen S, Yi Leung S, Nelson WJ, Brown PO "Parallels between Global Transcriptional Programs of Polarizing Caco-2 Intestinal Epithelial Cells In Vitro and Gene Expression Programs in Normal and Colon Cancer. (openwetware.org)
  • Induced pluripotent stem cells (iPSCs) generated from patient fibroblasts could potentially be used as a source of autologous cells for transplantation in retinal disease. (nature.com)
  • Using a patient's own cells for transplantation would avoid these pitfalls and is possible with induced pluripotent stem cells (iPSCs). (nature.com)
  • Derivation of autologous induced pluripotent stem cells (iPSCs) through direct reprogramming of easily accessible somatic cells holds the potential to transform the field of regenerative medicine. (biomedcentral.com)
  • Here, we summarize current reprogramming methodologies with a focus on the production of transgene-free or genetically unmanipulated iPSCs and highlight important technical details that ultimately may influence the biological properties of pluripotent stem cells. (biomedcentral.com)
  • In 2006, a major breakthrough was reported in Japan by Takahashi and Yamanaka, who described the generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts via over expression of defined transcription factors [ 2 ]. (biomedcentral.com)
  • Ideally, iPSC-based therapies in the future will rely on the isolation of skin fibroblasts or keratinocytes, their reprogramming into iPSCs, and the correction of the genetic defect followed by differentiation into the desired cell type and transplantation. (biomedcentral.com)
  • Several experimental strategies have been developed to derive iPSCs from differentiated somatic cells (summarized in Figure 1 ). (biomedcentral.com)
  • The cells are formally termed "iPSCs. (psychiatrictimes.com)
  • For reasons not fully understood, this integrated gene combination goes right to work reprogramming the skin, transforming it into a batch of versatile iPSCs. (psychiatrictimes.com)
  • Induced pluripotent stem cells (iPSCs) were discovered in 2006 by Shinya Yakama and his student Kazutoshi Takahashi in Japan. (ysjournal.com)
  • By taking somatic stem cells and activating specific genes [3], induced pluripotent stem cells (iPSCs) were originated, making these theories of regenerative treatments mentioned before a current reality. (ysjournal.com)
  • Induced Pluripotent Stem Cells (iPSCs) are cells derived from non-pluripotent cells, such as adult somatic cells, that are genetically manipulated so as to return to an undifferentiated, pluripotent state. (asu.edu)
  • Research on iPSCs, initiated by Shinya Yamanaka in 2006 and extended by James Thompson in 2007, has so far revealed the same properties as embryonic stem cells (ESCs), making their discovery potentially very beneficial for scientists and ethicists alike. (asu.edu)
  • GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
  • These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
  • The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)
  • To investigate underlying disease mechanisms, induced pluripotent stem cells (iPSCs) allow the generation of patient-derived neuronal cells in a dish. (biomedcentral.com)
  • The AD-iPSCs were differentiated into neuronal cells, in order to generate disease-specific protein association networks modeling the molecular pathology on the transcriptome level of AD, to analyse the reflection of the disease phenotype in gene expression in AD-iPS neuronal cells, in particular in the ubiquitin-proteasome system (UPS), and to address expression of typical AD proteins. (biomedcentral.com)
  • We detected the expression of p-tau and GSK3B, a physiological kinase of tau, in neuronal cells derived from AD-iPSCs. (biomedcentral.com)
  • Treatment of neuronal cells differentiated from AD-iPSCs with an inhibitor of γ-secretase resulted in the down-regulation of p-tau. (biomedcentral.com)
  • Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
  • However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
  • Progenitor cells can be far easier to handle in culture than ES cells. (ddw-online.com)
  • For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)
  • Therefore, the therapeutic use of appropriate populations derived from stem and progenitor cells has been considered for cell-based therapies for neurodegenerative diseases, primarily Parkinson's disease, and brain injuries. (ehu.eus)
  • Only from the primordial germ cells of the gonadal ridge (progenitor cells of egg and sperm cells) can pluripotent stem cells be cultivated under laboratory conditions. (understanding-stemcells.info)
  • The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)
  • These approaches result in the loss of all histological information pertaining to the in vivo locale of MaSC and progenitor cells. (frontiersin.org)
  • Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)
  • MaSC/progenitor cells were identified based on their ability to retain bromodeoxyuridine for an extended period. (frontiersin.org)
  • whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
  • Efficient commitment to functional CD34+ progenitor cells from human bone marrow mesenchymal stem-cell-derived induced pluripotent stem cells. (1library.org)
  • At first, the mesenchymal stem cell was thought to be the primary component of this undifferentiated cell type, however it is now evident that within the adipose extracellular matrix are also adipocytic precursors (known as progenitor cells) adherent to adipocytes, and in close approximation to a variety of additional undifferentiated multipotent and pluripotent cells, including pericytes and endothelial cells, all thought to play important roles in mesenchymal-stromal derived tissue regeneration. (journalofprolotherapy.com)
  • Unlike embryonic stem cells, the use of human adult stem cells in research and therapy is not considered to be controversial, as they are derived from adult tissue samples rather than human embryos designated for scientific research. (wikipedia.org)
  • Under normal conditions, tissue stem cells divide slowly and infrequently. (wikipedia.org)
  • The results from a study that used cells derived from a deceased patient's skin tissue has recently been published. (psychiatrictimes.com)
  • 2 It was reported that stem cells have an ability to transform themselves into different cell types, depending on the tissue, essentially at the scientist's whim. (psychiatrictimes.com)
  • When stem cells were first discovered in 1961 by the biophysicist James Till and the cellular biologist Ernest McCulloch, the scientific community became quickly enthusiastic about its coming possibilities: with the ability to self-renew and replace damaged tissue in the body, these new-known cells came to be the core of the upcoming field of medical treatment, the regenerative medicine. (ysjournal.com)
  • Speaking on disease treatments, these cells could be used to treat several types of diseases, such as diabetes and Parkinson's disease [4], since with them any type of tissue needed could be formed and used to replace a damaged one. (ysjournal.com)
  • E.g in cardiac therapies, the induced pluripotent stem cell line promotes cardiac tissue regeneration and when this one is applied to the organ, it leads to improvements in cardiac function [2]. (ysjournal.com)
  • Adult stem cell -An undifferentiated cell found in a differentiated tissue that can renew itself and (with certain limitations) differentiate to yield all the specialized cell types of the tissue from which it originated. (cellmedicine.com)
  • Bone marrow stromal cells -A stem cell found in bone marrow that generates bone, cartilage, fat, and fibrous connective tissue. (cellmedicine.com)
  • Mesenchymal stem cells -Cells from the immature embryonic connective tissue. (cellmedicine.com)
  • Practitioners of tissue engineering seek to repair, replace, maintain, and enhance the abilities of a specific tissue or organ by means of living cells. (asu.edu)
  • In particular, the advent of human embryonic stem cells followed by reprograming technologies for generation of induced pluripotent stem cells have instigated studies into modeling human brain development and disease by providing a means to simulate a human tissue otherwise completely or largely inaccessible to researchers. (ehu.eus)
  • Brain organoids mimic more closely the in vivo tissue composition and architecture and are more physiologically relevant than monolayer cultures. (ehu.eus)
  • Depending on the type of stem cell and its influence, they have the potential to develop into any tissue (embryonic stem cells) or certain specified tissue types (adult stem cells). (understanding-stemcells.info)
  • These stem cells have a potential that in the transition between embryonic and tissue stem cells. (understanding-stemcells.info)
  • However, they still grow faster than tissue stem cells, which are already defined as cell types of a particular tissue. (understanding-stemcells.info)
  • Tissue stem cells (= adult stem cells) are specific dividable cells in already mature tissues. (understanding-stemcells.info)
  • These adult stem cells serve self-renewal and the development of specialized tissue cell types. (understanding-stemcells.info)
  • Mesenchymal stem cells (MSC) are tissue stem cells originating in the mesoderm germ layer. (understanding-stemcells.info)
  • They are also called stromal cells and are very similar to connective tissue cells, the fibroblasts. (understanding-stemcells.info)
  • It has been demonstrated that MSC can differentiate into chondrocytes (cartilage-forming cells), osteoblasts (bone-forming cells) and adipocytes (adipose tissue cells). (understanding-stemcells.info)
  • Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
  • However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)
  • An immunologically identical cardiac tissue can be produced by direct reprogramming from the patients' tissue without the potential risk of tumorigenesis that occurs with induced pluripotent stem cells [ 2 ] and the ethical issue using embryonic stem cells. (thno.org)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • [6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
  • Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • Tissue extracts of Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)
  • ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
  • Mesenchymal stem cells (MSCs) are an example of tissue or 'adult' stem cells. (tomeipowered.com)
  • Explaining the difference between embryonic and cord tissue-derived stem cells. (tomeipowered.com)
  • Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
  • Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
  • Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
  • The culmination of these biological processes results in the replacement of normal skin structures with fibroblastic mediated scar tissue. (medscape.com)
  • MEFs overexpressing these genes showed epithelial cell characteristics, such as cobblestone appearance and E-cadherin positivity, and formed oral epithelial-like tissue under air-liquid interface culture conditions. (bvsalud.org)
  • Although stem cell aging leads to a decline in tissue homeostasis and regenerative capacity, it remains unclear whether salivary gland stem cell function changes during this process. (bvsalud.org)
  • Klapholz-Brown Z, Walmsley GG, Nusse YM, Nusse R, Brown PO "Transcriptional program induced by wnt protein in human fibroblasts suggests mechanisms for cell cooperativity in defining tissue microenvironments. (openwetware.org)
  • Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)
  • One explanation for deficient repair is when undifferentiated adult stem repair cells are inadequate in number or cannot be stimulated within the damaged tissue site. (journalofprolotherapy.com)
  • With improved understanding of tissue healing and regeneration, stem cell Prolotherapy is gaining significant clinical importance and potential. (journalofprolotherapy.com)
  • Integrated spatial multiomics reveals fibroblast fate during tissue repair. (stanford.edu)
  • In the skin, tissue injury results in fibrosis in the form of scars composed of dense extracellular matrix deposited by fibroblasts. (stanford.edu)
  • Here, we present a multimodal -omics platform for the comprehensive study of cell populations in complex tissue, which has allowed us to characterize the cells involved in wound healing across both time and space. (stanford.edu)
  • We employ a stented wound model that recapitulates human tissue repair kinetics and multiple Rainbow transgenic lines to precisely track fibroblast fate during the physiologic response to skin injury. (stanford.edu)
  • Adult stem cells are undifferentiated cells, found throughout the body after development, that multiply by cell division to replenish dying cells and regenerate damaged tissues. (wikipedia.org)
  • In addition, as the genetic identity of the donor egg from which the ESCs are derived most likely will differ from that of potential recipients, patients who receive ESC-derived cells or tissues may face the same complications that result from organ transplantation (for example, immunorejection, graft-versus-host disease, and need for immunosuppression). (biomedcentral.com)
  • Astrocyte -One of the large neuroglia cells of neural tissues. (cellmedicine.com)
  • Cell-based therapies -treatment in which stem cells are induced to differentiate into the specific cell type required to repair damaged or depleted adult cell populations or tissues. (cellmedicine.com)
  • Their team focuses on using human induced pluripotent stem cells to grow human tissues inside pigs. (the-scientist.com)
  • Human pluripotent stem cells harbor the potential to provide an inexhaustible supply of donor cells or tissues or organs for transplantation," Wu wrote in an email. (the-scientist.com)
  • This can range from the relatively pedestrian, such as a person who received a bone marrow transplant, to creatures that seem more at home in science fiction, such as animals containing cells or tissues belonging to other species. (the-scientist.com)
  • Shih CC, Forman SJ, Chu P, Slovak M. Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice. (razavihospital.ir)
  • Unguided differentiation confirms HiPSC ability to generate cell derivatives of tissues arising from the three embryonic layers. (cellapplications.com)
  • Body cells that can make copies of themselves and differentiate into different cell types or tissues are generally referred to as stem cells. (understanding-stemcells.info)
  • Their differentiation potential is limited to the maturation of genetically determined tissues in the environment of which (niches) they are found, for example, in the skin, the liver, the intestine or the hematopoietic system. (understanding-stemcells.info)
  • Even after we have fully developed, adult stem cells stick around and work on regenerating tissues as they are damaged. (medium.com)
  • MSCs make the different specialized cells found in the skeletal tissues. (tomeipowered.com)
  • Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
  • Herein, we show that salivary gland-like cells can be induced from epithelial tissues that were transdifferentiated from mouse embryonic fibroblasts (MEFs). (bvsalud.org)
  • Recognition of the vast number of undifferentiated cells associated with the stromal vascular fraction has resulted in extensive research demonstrating the heterogeneity of such cells, and their ability to participate in production of all mesodermal-derived tissues. (journalofprolotherapy.com)
  • It was found out that, with the expression of only four genes, Oct3/4, Sox2, c-Myc and Klf4, somatic stem cells could be reprogrammed into the pluripotent-like state. (ysjournal.com)
  • In 2013, George Church and his colleagues at Harvard University in Cambridge, Massachusetts published RNA-Guided Human Genome Engineering via Cas 9, in which they detailed their use of RNA-guided Cas 9 to genetically modify genes in human cells. (asu.edu)
  • Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
  • Genes Cells. (razavihospital.ir)
  • In 2006, the Japanese scientist Shinya Yamanaka used viruses to introduce the genes Oct4, Sox2, cMyc and Klf4 into mouse body cells for the first time, activating quiescent developmental genes. (understanding-stemcells.info)
  • Transcriptome analysis of AD-iPS derived neuronal cells revealed significant changes in the expression of genes associated with AD and with the constitutive as well as the inducible subunits of the proteasome complex. (biomedcentral.com)
  • Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
  • Genes expressed in LRECe revealed retention of some stem-like properties along with up-regulation of differentiation factors. (frontiersin.org)
  • Dr. Yamanaka had found a way to convert a mature skin cell into a stem cell by injecting just a few genes. (medium.com)
  • First, Dr. Yamanaka's team recognized a host of genes (24 in total) that were involved in the maintenance of ES cell phenotypes and genes that were only expressed in ES cells. (medium.com)
  • Next, they used vectors known as retroviruses to introduce these genes into cells, after which they tested pluripotency by observing the reaction of the cell to specific compounds. (medium.com)
  • The transcripts of most expressed genes, in a variety of cell types, were shown to be methylated, indicating that m6A modifications are widespread. (pharmaceuticalintelligence.com)
  • The hiSGs exhibit properties similar to those of embryonic salivary glands and are a valuable tool for the functional analysis of genes during development. (bvsalud.org)
  • We introduced four genes, Dnp63a, Tfap2a, Grhl2, and Myc (PTMG) that are known to transdifferentiate fibroblasts into oral mucosa-like epithelium in vivo into MEFs. (bvsalud.org)
  • The spheres expressed genes encoding basal/myoepithelial markers, cytokeratin 5, cytokeratin 14, acinar cell marker, aquaporin 5, and the myoepithelial marker α-smooth muscle actin. (bvsalud.org)
  • Multipotency or multidifferentiative potential is the ability to generate progeny of several distinct cell types, (for example glial cells and neurons) as opposed to unipotency, which is the term for cells that are restricted to producing a single cell type. (wikipedia.org)
  • It involves answering some simple questions: What if you took the skin cells from patients who had schizophrenia and turned them into neurons? (psychiatrictimes.com)
  • In the nineteenth century, reticular theory aimed to describe the properties of neurons, the specialized cells which make up the nervous system, but was later disconfirmed by evidence. (asu.edu)
  • HiPSC-derived Neural Stem Cells (L) and Neurons (R) . i-HNSC stained w/ Nestin (neural stem cell marker, green), SOX 2 (stem cell marker, red) & DAPI (nuclear stain, blue). (cellapplications.com)
  • Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
  • First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
  • Second, we used directly converted neurons from sporadic patient fibroblasts to study of age-related disease relevant pathology. (lu.se)
  • Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
  • This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
  • Unlike many other kinases, GSK3B is believed to be permanently active in resting cells and in neurons without extracellular stimulation and can be inactivated by Ser9 phosphorylation [ 14 ]. (biomedcentral.com)
  • Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)
  • Fetal stem cells are isolated from older embryos or fetuses (five to nine weeks old) that have miscarried, or they are harvested after termination of pregnancy. (understanding-stemcells.info)
  • Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
  • Discoveries in recent years have suggested that adult stem cells might have the ability to differentiate into cell types from different germ layers. (wikipedia.org)
  • Stem cells from the bone marrow, which is derived from mesoderm, can differentiate into liver, lung, GI tract, and skin, which are derived from endoderm and mesoderm. (wikipedia.org)
  • Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
  • HiPSC differentiate into cell derivatives from the 3 embryonic layers: Neuronal marker beta III tubulin (TUJ1), Smooth Muscle Actin (SMA) and Hepatocyte Nuclear Factor 3 Beta (HNF3b). (cellapplications.com)
  • They can no longer differentiate into almost all cells as embryonic stem cells can. (understanding-stemcells.info)
  • As the development process continues, these stem cells begin to differentiate or turn into cells that carry out only specific functions, based on their different chemical signals that they're exposed to. (medium.com)
  • Mesenchymal stem cells (MSCs), or stromal stem cells, can differentiate into many different types of … In contrast, treatments that utilize MSCs from a patientâ s fat (adipose) sample have shown weak or unreliable responses. (tomeipowered.com)
  • Concomitantly, the ability of MSCs to differentiate and give rise to multiple cell types such as adipocytes, chondrocytes, and osteoblasts … These transplants vastly increase the bodyâ s natural healing abilities and have potent anti-inflammatory and immunosuppressive responses.Â. (tomeipowered.com)
  • Mesenchymal Stem Cells Instruction Manual Product Description Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are self-renewing multipotent cells that can differentiate into a wide variety of cell types. (tomeipowered.com)
  • The potential use of embryonic stem cells (ESCs) for cell replacement therapies is limited by ethical concerns and the technical hurdles associated with their isolation from human embryos. (biomedcentral.com)
  • Their exploration has allowed the study of cells with similar states of pluripotency as found in embryonic stem cells (ESCs), breaking the ethical barrier that interfered with the study of ESCs, as well as their use in therapies, disease modeling and drug development. (ysjournal.com)
  • Pluripotent stem cells form part of the blastocyst, more specifically, the embryoblast, in which its cells are called embryonic stem cells (ESCs) and are considered pluripotent because they have various possible fates. (ysjournal.com)
  • The idea of inducing a pluripotent stem cell similar to the ESCs was executed by Shinya Yakama and his student Kazutoshi Takahashi at Kyoto University, Japan, in 2006. (ysjournal.com)
  • Molecular cues for pluripotency, genetic reprogramming of adult stem cells, and widely multipotent adult cells. (razavihospital.ir)
  • They are therefore referred to as multipotent, not pluripotent. (understanding-stemcells.info)
  • Mesenchymal stem/stromal cells (MSCs) are multipotent cells that are emerging as the most promising means of allogeneic cell therapy. (tomeipowered.com)
  • With high levels of platelet-derived growth factors and cytokines, this combination provides both a living bioscaffold and a multipotent cell replenishment source useful for enhanced musculoskeletal healing. (journalofprolotherapy.com)
  • Through established protocols 2 , fibroblasts from a skin biopsy can be returned to a pluripotent state and serve as a renewable, autologous source of replacement cells that avoids the ethical complications of hESCs. (nature.com)
  • Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
  • The overall aim of this thesis has been to assess the potential of autologous grafting in cell replacement therapy for PD. (lu.se)
  • Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)
  • Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
  • Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)
  • 1, 2 Recently, Prolotherapists have begun to utilize the potential of autologous adipose (fat)-derived stem/stromal cells (AD-SC) within non-manipulated fat graft scaffolding, combined with high-density PRP concentrates (HD-PRP) to provide a potent biological therapeutic combination. (journalofprolotherapy.com)
  • Exploring the genetic, molecular and biological features of neural differentiation process from embryonic and patient-specific stem cells may offer an unprecedented chance to understand normal and abnormal human brain development and diseases, with implications for drug development and potential cellular replacement therapy. (yale.edu)
  • The idea that cellular survival and growth could be maintained outside the body was recognized as possible almost hundred years ago when the German zoologist Wilhelm Roux described a successful experiment where he cultured chick neural crest in warm saline for a few days [ 1 ]. (intechopen.com)
  • Initially, two‐dimensional neural cultures presented an innovative landmark for investigating human neuronal and, more recently, glial biology, as well as for modeling brain neurodevelopmental and neurodegenerative diseases. (ehu.eus)
  • Soen Y, Mori A, Palmer TD, Brown PO "Exploring the regulation of human neural precursor cell differentiation using arrays of signaling microenvironments. (openwetware.org)
  • Ying QL, Stavridis M, Griffiths D, Li M, Smith A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent Ferro F et al. (razavihospital.ir)
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
  • Scalable passaging of adherent human pluripotent stem cells. (1library.org)
  • During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
  • During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
  • c-Myc has many downstream targets that enhance cell proliferation and transformation, which may also play a major role in iPS cell generation. (medium.com)
  • Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
  • To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
  • This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
  • This has allowed us to reveal potential mechanisms controlling fibroblast fate during migration, proliferation, and differentiation following skin injury, and thereby reexamine the canonical phases of wound healing. (stanford.edu)
  • Pluripotency was demonstrated by teratoma assay conducted in immunodeficient mice (Charles River, Wilmington, MA) and immunocytochemistry staining for pluripotency markers. (nature.com)
  • Reprogramming triggers a cascade of evident changes in the host cells that are recognizable morphologically and through a combination of markers and pluripotency assays. (cellapplications.com)
  • From this, he inferred that there must be some factors in these undifferentiated cells that maintain the pluripotent (capable of differentiating into many other cell types) state , and hypothesized that these same factors could also induce pluripotency in somatic cells. (medium.com)
  • Oct3/4 and Sox2 code for key transcription factors important for inducing pluripotency to cause the development of what they call induced pluripotent stem cells (iPS cells) . (medium.com)
  • State-of-the art technologies are used to investigate global molecular changes that arise during neuronal fate specification and differentiation from pluripotent embryonic stem (ES) cells. (yale.edu)
  • To better understand the neuronal specificity of IKAP loss, we examined expression of miRNAs in human olfactory ecto-mesenchymal stem cells (hOE-MSCs) from five control individuals and five FD patients. (biologists.com)
  • Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)
  • Moreover, an AD-related protein interaction network composed of APP and GSK3B among others could be generated using neuronal cells differentiated from two AD-iPS cell lines. (biomedcentral.com)
  • Alzheimer's disease (AD) is characterized by histopathological changes, designated as senile plaques and fibrillary deposits, which ultimately lead to the death of neuronal cells in particular in the cerebral cortex of the brain [ 1 , 2 ]. (biomedcentral.com)
  • neuronal-like cells. (tomeipowered.com)
  • The use of various types of stem cells for research purposes to make disease "models" in the lab for regenerative medicine and for "therapies" to cure sick patients for diseases is constantly in the news. (lifeissues.net)
  • However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. (lww.com)
  • The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)
  • Among the cell types that can be used for this purpose, mesenchymal stem cells (MSCs) are considered as promising source of stem cells in personalized cell-based therapies. (tomeipowered.com)
  • Human embryonic stem cell research is ethically and politically controversial because since it involves the destruction of human embryos that have the potential to become human beings, it is the topic of several debates addressing morals and beliefs. (ysjournal.com)
  • Researchers in Jun Wu's lab compare normal mouse embryos (left) to horse-mouse chimeric embryos (right) to identify barriers to interspecies chimerism. (the-scientist.com)
  • Moscona A. Cell suspensions from organ rudiments of chick embryos. (razavihospital.ir)
  • The only problem was, these stem cells could only be obtained from embryos . (medium.com)
  • But there is obviously a huge ethical problem with obtaining stem cells from embryos. (medium.com)
  • The first of which is their ability to divide or self-renew indefinitely, and the second their ability to generate all the cell types of the organ from which they originate, potentially regenerating the entire organ from a few cells. (wikipedia.org)
  • To generate healthy patient-derived cells, mutations might be repaired with new gene-editing technology based on the bacterial system of clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9, thereby yielding grafts that require no patient immunosuppression. (nature.com)
  • The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)
  • The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
  • Stem cells are able to generate daughter cells, which themselves possess stem cell properties. (understanding-stemcells.info)
  • If this also becomes possible in the future with reprogrammed human stem cells, it may be possible to generate a human embryo without the need for natural germ cells. (understanding-stemcells.info)
  • The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
  • Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. (lww.com)
  • 1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
  • osteoblasts, chondrocytes, endothelial-cells and also non-mesoderm-type lineages e.g. (tomeipowered.com)
  • 2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
  • Since their discovery, stem cells have ushered in a new era in regenerative medicine. (ehu.eus)
  • In particular, due to the limited regenerative capacity of the adult nervous system, the therapeutic potential of stem cells has opened up new perspectives for development of treatment strategies for brain repair after injury or disease. (ehu.eus)
  • Cardiovascular diseases often cause substantial heart damage and even heart failure due to the limited regenerative capacity of adult cardiomyocytes. (thno.org)
  • Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)
  • The therapeutic goal of regenerative wound healing has remained elusive, in part because principles of fibroblast programming and adaptive response to injury remain incompletely understood. (stanford.edu)
  • Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
  • Nano-thin and nano-porous membranes in the culture system facilitated interactions between fibroblasts and cardiomyocytes in coculture. (thno.org)
  • The cardiac-mimetic culture system may serve as an effective tool for producing a feasible number of reprogrammed cardiomyocytes from fibroblasts. (thno.org)
  • In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)
  • Evaluation of the cardiotoxicity of mitragynine and its analogues using human induced pluripotent stem cell-derived cardiomyocytes. (1library.org)
  • Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression. (1library.org)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • For example, when the variant H2AZ is incorporated into the nucleosome it increases the accessibility of DNA to specific transcription factors and promotes the conversion of somatic cells to iPS cells. (jove.com)
  • Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)
  • Czyz J, Wobus A. Embryonic stem cell differentiation: the role of extracellular factors. (razavihospital.ir)
  • F urther complicating matters, human ES cells are typically co-cultured with feeder layers of mouse fibroblast cells. (ddw-online.com)
  • Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)
  • The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)
  • However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
  • Feeder layer -Cells used in co-culture to maintain pluripotent stem cells. (cellmedicine.com)
  • Our HiPSCs display classic pluripotent stem cell morphology, with a high nucleus to cytoplasm size ratio, as well as they are amenable to be cultivated in serum-free media, independent of feeder cells and of feeder-conditioned media as colonies or high density monolayers. (cellapplications.com)
  • Embryonic germ cells -Cells found in a specific part of the embryo/fetus called the gonadal ridge that normally develop into mature gametes. (cellmedicine.com)
  • Germ cells are cells that form the germ line and are already predetermined for development of the next cell generation in the early embryo. (understanding-stemcells.info)
  • In particular, scientific developments in areas such as iPS cells open new possibilities of research and, at mid term, of therapeutic applications, but they also bring new ethical challenges and problems requiring further reflection and debate. (lifeissues.net)
  • Potentiality of embryonic stem cells: an ethical problem even with alternative stem cell sources. (razavihospital.ir)
  • Recent clinical trials evaluating allogeneic retinal grafts derived from human embryonic stem cells (hESCs) show the procedure to be safe and potentially effective 1 . (nature.com)
  • A deep commitment to academic medicine has fundamentally influenced my research interests, from years of basic molecular neuroscience research to becoming a double-boarded physician in Adult Neurology and Clinical Genetics with a continuing involvement in basic and translational research. (yale.edu)
  • To define better cellular events that are specifically vulnerable to WRN deficiency, I used RNA interference (RNAi) to silence the expression of Wrn gene and other RecQ helicases, linked to different clinical phenotypes, in primary human fibroblasts. (yale.edu)
  • Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
  • Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
  • Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
  • These pluripotent cells, as they form the embryo, can turn into any other type of cell that exists in the human body, except for the totipotent type. (ysjournal.com)
  • Blastocyst -A preimplantation embryo of about 150 cells. (cellmedicine.com)
  • Embryonic stem cells -Primitive (undifferentiated) cells from the embryo that have the potential to become a wide variety of specialized cell types. (cellmedicine.com)
  • These cells give rise to the embryonic disk of the later embryo and, ultimately, the fetus. (cellmedicine.com)
  • We think that the developmental cues that exist in the pig will help to guide the human cells inside the porcine embryo. (the-scientist.com)
  • The removal of the hES cells leads to loss of the embryo. (understanding-stemcells.info)
  • The production of hES cells in this manner is considered ethically unacceptable in Germany and is prohibited (Embryo Protection Act). (understanding-stemcells.info)
  • And so, Dr. Yamanaka set out to find a way to obtain stem cells without having to extract them from the embryo. (medium.com)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)
  • Fundamental questions in organogenesis are related to the identification of a simplified model which is able to mimic the molecular mechanisms involved in pattern organization and cell fate determination as well. (razavihospital.ir)
  • In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)
  • Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
  • The adult stem cell can be labeled in vivo and tracked, it can be isolated and then transplanted back into the organism, and it can be isolated in vivo and manipulated with growth hormones. (wikipedia.org)
  • 2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
  • In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
  • I'll start with a brief review of hiPSCs (human inducible pluripotent stem cells), then move to the data. (psychiatrictimes.com)
  • Once there was a reliable way to manufacture healthy hiPSCs, scientists could turn their attention toward their most important goal: learning how to create the cell type they wanted to study. (psychiatrictimes.com)
  • Post-thawing viability of HiPSCs is typically higher than 70%, and HiPSC have demonstrated coherent pluripotent behavior over more than 60 passages. (cellapplications.com)
  • But in order to become a part of medical history, parahuman reproduction and human genetic engineering must circumvent the recalcitrance of an antiquated culture. (lifeissues.net)
  • But what is not getting such wide reporting is the use of pluripotent stem cells (as well as many other types of cells and genetic engineering techniques) for reproductive purposes . (lifeissues.net)
  • Church and his team used RNA-guided Cas 9 technology to edit the genetic information in human cells. (asu.edu)
  • Since the virus does not go through a DNA phase, its genetic material and transgenes do not integrate into the host cell genome. (cellapplications.com)
  • From previous experiments, Dr. Yamanaka had realized that somatic cells (or normal, differentiated cells of the body) could be reprogrammed by either transferring their genetic material into oocytes (egg cells) or by fusing them with embryonic stem cells . (medium.com)
  • Genetic background drives transcriptional variation in human induced pluripotent stem cells. (1library.org)
  • However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
  • In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
  • We still have a lot of gaps in our knowledge about gut microbiomes, but with the publicly available database of cultured mouse gut bacteria and their genetic material, we are now a little closer to our goal," Thomas Clavel from the TUM stated enthusiastically. (vetscite.org)
  • Hematopoietic stem cell -A stem cell from which all red and white blood cells develop. (cellmedicine.com)
  • Microenvironment -The molecules and compounds such as nutrients and growth factors in the fluid surrounding a cell in an organism or in the laboratory, which are important in determining the characteristicsof the cell. (cellmedicine.com)
  • To circumvent these obstacles, considerable effort has been invested in attempting to derive ESC-like cells by reprogramming somatic cells to an embryonic state. (biomedcentral.com)
  • Although exciting results have been achieved by means of somatic cell nuclear transfer, cell fusion, and culture-induced reprogramming [ 1 ], these procedures are technically demanding and inefficient and therefore unlikely to become a common approach for producing patient-specific pluripotent cells. (biomedcentral.com)
  • To gain new insights into pathogenesis of specific neurological and neurodevelopmental disorders, such as autism, pluripotent stem cells are derived by reprogramming of differentiated, mature cells of affected patients. (yale.edu)
  • Our HiPSC are generated with the RNA-based Sendai virus to deliver reprogramming factors to donor skin fibroblasts. (cellapplications.com)
  • Induced pluripotent stem cells (iPS cells) are stem cells that are created by artificial reprogramming of human body cells. (understanding-stemcells.info)
  • The direct cardiac reprogramming of fibroblasts could be a promising therapeutic option for these patients. (thno.org)
  • The cellular interactions and electric stimulation supplied by the culture system dramatically enhanced the cardiac reprogramming efficiency of cardiac-specific transcriptional factor-transfected fibroblasts. (thno.org)
  • Direct cardiac reprogramming of fibroblasts is an attractive therapeutic strategy for treating cardiovascular diseases because the adult heart has a low regeneration capacity [ 1 ]. (thno.org)
  • Recent work has demonstrated that the epigenome is already rejuvenated by the maturation phase of somatic cell reprogramming, which suggests full reprogramming is not required to reverse ageing of somatic cells. (elifesciences.org)
  • This study describes a novel "maturation phase transient reprogramming" (MPTR) method to restore the epigenome of cells to a more youthful state. (elifesciences.org)
  • therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells. (jove.com)
  • Ewen Callaway investigates a new field in cell biology. (nature.com)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • Development: For advances in developmental biology and stem cells. (lu.se)
  • Stem cell technologies have opened up new avenues in the study of human biology and disease. (ehu.eus)
  • They therefore represent a more realistic cellular environment for modeling the cell biology and pathology of the nervous system. (ehu.eus)
  • To first explain what stem cells are (for those of you that don't have much biology experience), we're going to go back in time to when you were just a small mass of cells in your mother's womb. (medium.com)
  • In normal cells, glucose participates in cellular energy production through glycolysis as well as through its complete catabolism via the tricarboxylic acid (TCA) cycle and oxidative phosphorylation (OXPHOS). (hindawi.com)
  • In normal cells, the fate of pyruvate depends on many factors, one of which is oxygen availability. (hindawi.com)
  • Their study "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors" is considered one of the most important findings of the past century and made Dr. Yakama win the Nobel Prize in Physiology or Medicine of 2012. (ysjournal.com)
  • Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. (razavihospital.ir)
  • The interplay between mechanical strain and inflammation in pulmonary fibrosis remain incompletely understood.APPROACH: In this study, we used conditional KO mice to block mechanotransduction by knocking out FAK (Focal Adhesion Kinase) expression in fibroblasts, followed by induction of pulmonary fibrosis using bleomycin. (stanford.edu)
  • This article will examine advances taking place on both the lab bench and the pharmaceutical production floor that are helping to bring the promise of stem cells closer to reality. (ddw-online.com)
  • However, advances in stem cell research in the 2000s revolutionized the field, opening up new possibilities and new applications for multispecies organism research. (the-scientist.com)
  • Advances in 3D non-destructive, non-invasive analysis methods and improvements in the multi-scale techniques and bioreactors to obtain test and culture 3D cell aggregates have been made. (razavihospital.ir)
  • CSCs take charge of a cancer hierarchy, harboring stem cell-like properties involving self-renewal and aberrant differentiation potential. (bmbreports.org)