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Induced Pluripotent Stem CellsPluripotent Stem CellsEmbryonic Stem CellsDermisNuclear ReprogrammingStem CellsCell DifferentiationSkinOctamer Transcription Factor-3Surgical TapeHair FollicleCell DedifferentiationSOXB1 Transcription FactorsRegenerative MedicineStem Cell TransplantationCell Culture TechniquesEmbryoid BodiesCells, CulturedFeathersFibroblastsEpidermisFetusHematopoietic Stem CellsCell LineageTeratomaAdult Stem CellsStem Cell ResearchNeural Stem CellsGerm LayersFetal Tissue TransplantationFeeder CellsComplicityStage-Specific Embryonic AntigensKruppel-Like Transcription FactorsCell- and Tissue-Based TherapyKeratinocytesLentivirusAborted FetusCell LineStem Cell NicheMultipotent Stem CellsResearchHematopoietic Stem Cell TransplantationCell ProliferationEmbryo, MammalianEpigenesis, GeneticMesenchymal Stromal CellsGene Expression Regulation, DevelopmentalGene Expression ProfilingGerm CellsRegenerationHomeodomain ProteinsCell SeparationWound HealingMyocytes, CardiacPregnancyMice, SCIDProto-Oncogene Proteins c-mycDirected Tissue DonationMesenchymal Stem Cell TransplantationTranscription FactorsTransduction, GeneticFlow CytometryKaryotypingTissue EngineeringBiological MarkersReverse Transcriptase Polymerase Chain ReactionMaternal-Fetal ExchangePlacentaGenetic VectorsGene Expression RegulationTransgenesLifeTotipotent Stem CellsNeuronsModels, BiologicalEndodermStem Cell FactorActivinsCell TransdifferentiationCoculture TechniquesChimeraX Chromosome InactivationBone Marrow CellsPersonhoodPhenotypeHematopoiesisEmbryo ResearchTranslational Medical ResearchGene ExpressionSignal TransductionNeoplastic Stem CellsBeginning of Human LifeImmunohistochemistryBone Morphogenetic Protein 4Mice, Inbred C57BLLeukemia Inhibitory FactorKaryotypeAmniotic FluidCell Engineering
FibroblastsBovine serumEndothelial cellsDifferentiationMSCsVitroIPSCsMouse embryonicRegenerativeVivoPapilla cellsDifficult to reprogramRegenerationProgenitor cellsDifferentiate2023ProliferationAutologousEpithelialFibroblastReprogramming of somatic cellsPluripotencyESCsAdipocytesPlatelet-rich plasmaBlood vesselsConnective tissueFound in bone marrowEmbryonic stemSomatic cellsAdipose derived stemIPSCOrganoidsSevereNeonatalCentreMechanismsBoneHair folliclesAndrogenetic alopecia2022BiologyCrucialPlacentalMicroenvironmentMultipotent cellsMethodsCardiacAdherentClinicalTherapeuticAngiogenesisImplantationExtracellular vesiclesInductionVascular smoothSquamous Cell CarcGenesWoundLiver
Fibroblasts20
  • iPS were generated via a platelet lysate or fetal bovine serum-based culturing protocol from matched dermal, buccal and gingival human fibroblasts, isolated from healthy donors ( n = 2) after informed consent, via episomal plasmid transfection. (biomedcentral.com)
  • Furthermore, oral fibroblasts generally required more attempts for successful iPS generation as opposed to dermal fibroblasts. (biomedcentral.com)
  • The oral fibroblasts generally proved to be more difficult to reprogram than dermal fibroblasts. (biomedcentral.com)
  • Xeno-free dermal, buccal and gingival fibroblasts can successfully generate iPS with a comparable genotype/phenotype to their xenogenic counterparts. (biomedcentral.com)
  • They include endothelial cells, fibroblasts, and keratinocytes. (frontiersin.org)
  • In vitro experiments withcardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibilityof BC-Ppy composites. (ibecbarcelona.eu)
  • In this study, employing iPS technology, we derived and characterized iPSCs from dermal fibroblasts of an 82-year-old female patient affected by sporadic AD. (biomedcentral.com)
  • Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. (elifesciences.org)
  • 2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
  • Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
  • The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
  • In the skin, tissue injury results in fibrosis in the form of scars composed of dense extracellular matrix deposited by fibroblasts. (stanford.edu)
  • Bovine Dermal Fibroblasts are isolated from fetal bovine skin, cryopreserved at passage one and delivered frozen. (accegen.com)
  • Scientists at King s College London have, for the first time, identified the unique properties of two different types of cells, known as fibroblasts, in the skin one required for hair growth and the other responsible for repairing skin wounds. (regenerativemedicine.net)
  • Fibroblasts are a type of cell found in the connective tissue of the body s organs, where they produce proteins such as collagen. (regenerativemedicine.net)
  • It is widely believed that all fibroblasts are the same cell type. (regenerativemedicine.net)
  • However, a study on mice by researchers at King s indicates that there are at least two distinct types of fibroblasts in the skin: those in the upper layer of connective tissue, which are required for the formation of hair follicles and those in the lower layer, which are responsible for making most of the skin s collagen fibers and for the initial wave of repair of damaged skin. (regenerativemedicine.net)
  • It is possible that this reflects a loss of upper dermal fibroblasts and therefore it may be possible to restore the skin s elasticity by finding ways to stimulate those cells to grow. (regenerativemedicine.net)
  • Illustration: Skin cells: fibroblasts shown in green. (regenerativemedicine.net)
  • In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
Bovine serum4
  • Therefore, we aimed to compare the efficiency of using humanized culture conditions, specifically human platelet lysate to fetal bovine serum, for iPS generation from different sources, and to evaluate their stemness. (biomedcentral.com)
  • Although exact figures are difficult to obtain, some have estimated that one million foetal cows are sacrificed each year to obtain the world's supply of foetal bovine serum, used to grow cultured cells. (wikipedia.org)
  • Components MODM (500ml) consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. (3hbiomedical.com)
  • Furthermore, conventional MSC expansion with fetal bovine serum (FBS) causes immune- and inflammation-related problems following the exposure of xenogeneic pathogens from FBS [ 5 ]. (biomedcentral.com)
Endothelial cells9
  • 2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
  • Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. (lww.com)
  • 1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
  • 19 - 21 Other signals, such as those from endothelial cells (ECs) located at the DP, are also essential for HF maintenance. (benitonovas.com)
  • At first, the mesenchymal stem cell was thought to be the primary component of this undifferentiated cell type, however it is now evident that within the adipose extracellular matrix are also adipocytic precursors (known as progenitor cells) adherent to adipocytes, and in close approximation to a variety of additional undifferentiated multipotent and pluripotent cells, including pericytes and endothelial cells, all thought to play important roles in mesenchymal-stromal derived tissue regeneration. (journalofprolotherapy.com)
  • Endothelial cells, which form the inner lining of microvessels, create this semipermeable barrier via a combination of cellular junctions, their glycocalyx, and the basement membrane that consists of vessel-specific extracellular matrix (ECM) [ 2 ]. (biomedcentral.com)
  • Embedded within the basement membrane and in direct contact with endothelial cells are pericytes, which are crucial for microvessel maturation, maintenance, as well as proper function. (biomedcentral.com)
  • The microvascular system and especially endothelial cells play a major role in inflammation, act as an endocrine organ and tightly regulate blood coagulation [ 1 ]. (biomedcentral.com)
  • This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. (thermofisher.com)
Differentiation21
  • Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
  • Here we show the differentiation of patient specific iPSCs derived from a patient with SFTPB deficiency into lung organoids with mesenchymal and epithelial cell populations from both the proximal and distal portions of the human lung. (nature.com)
  • After differentiating the mutant and corrected cells into lung organoids, we show expression of SFTPB mRNA during endodermal and organoid differentiation but the protein product only after organoid differentiation. (nature.com)
  • Although many differentiation protocols in the literature have been successful in mimicking lung development from stem cells, there has not been an examination of how a specific mutation impacts the differentiation process including its effects on the early endoderm, as well as the proximal and distal lung epithelial cell populations in the lung organoids. (nature.com)
  • In this Chapter, we dissect the proof-of-principle studies that demonstrated that cellular reprogramming to pluripotency can be induced in vivo, in spite of unfavorable pro-differentiation signals present within the tissues. (springer.com)
  • Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity. (springer.com)
  • In this work, we show that translocation of the Pou5f1 gene fragment purported to span all essential cis-elements, including the well-known distal and proximal enhancers (DE and PE), into the Rosa26 locus impairs the self-renewal of mouse embryonic stem cells (ESCs) in the naïve pluripotency state, as well as their further advancement through the formative and primed pluripotency states, inducing overall differentiation failure. (bvsalud.org)
  • Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
  • Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
  • Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics. (razavihospital.ir)
  • Czyz J, Wobus A. Embryonic stem cell differentiation: the role of extracellular factors. (razavihospital.ir)
  • Engineering the embryoid body microenvironment to direct embryonic stem cell differentiation. (razavihospital.ir)
  • Kurosawa H. Methods for inducing embryoid body formation: in vitro differentiation system of embryonic stem cells. (razavihospital.ir)
  • However, the limited amount of stem cells and their tendency to undergo spontaneous differentiation upon extended propagation in vitro restrict their practical application. (biomedcentral.com)
  • Furthermore, MDLS could promote teratoma formation and enhanced differentiation potential of P19 cells in vivo. (biomedcentral.com)
  • Our mesenchymal stem cell osteogenic differentiation medium has been optimized for the osteogenic differentiation of mesenchymal stem cell (MSCs) in tissue culture plastic-ware. (3hbiomedical.com)
  • Efficiency of osteogenic differentiation depends on the quanlity of the mesenchymal stem cells. (3hbiomedical.com)
  • Mesenchymal stem cell Osteogenic Differentiation Medium (MODM) is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals. (3hbiomedical.com)
  • No. 0025), 5 ml of mesenchymal stem cell osteogenic differentiation supplement (MODS, Cat. (3hbiomedical.com)
  • Human mesenchymal stem cells (MSCs) are therapeutic for clinical applications because of their excellent immunomodulatory and multiple lineage differentiation abilities at tissue injury sites. (biomedcentral.com)
  • MSCs have limited pluripotency and capacity for cell differentiation compared to embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). (biomedcentral.com)
MSCs12
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • We previously demonstrated that mesenchymal stem cells (MSCs) ameliorated experimental autoimmune uveoretinitis (EAU) in rats. (nature.com)
  • Our group recently reported that CD73 on the cell membrane also contributes to the immunomodulatory capacity of MSCs 3 , suggesting that MSCs exert their immunomodulatory properties in a multifactorial manner. (nature.com)
  • Matrix metalloproteinase 1(MMP1), which was downregulated in myelodysplastic syndromes (MDS)-mesenchymal stromal cells (MSCs), was identified as an inhibitory factor of MDS cell proliferation. (cusabio.com)
  • 4 HFSCs are located in the bulge region of the follicle and they interact with mesenchymal SCs (MSCs) located in the dermal papilla (DP). (benitonovas.com)
  • 24 - 26 ECs may provide signals for BMP receptor activation in DPCs similar to those signals that promote survival of MSCs in human embryoid bodies composed of multipotent cells. (benitonovas.com)
  • 6,7 Mesenchymal stem cells (MSCs) have also been isolated from other sources, such as bone marrow, 8 the placenta, 9 muscle, 10 or blood. (jcadonline.com)
  • 11 However, the greater the abundance of adipose tissue in the human body, the significantly higher the yield of MSCs is compared to other tissues. (jcadonline.com)
  • Here, we demonstrated a preconditioning strategy with trophoblast stem cell-derived extracellular vesicles (TSC-EVs) to boost the proliferation and regenerative capacity of MSCs. (biomedcentral.com)
  • We employed cell proliferation analyses such as CCK8 and BrdU assays to determine the proliferation-promoting role of TSC-EVs on MSCs. (biomedcentral.com)
Vitro20
  • Two major alternatives to in vivo animal testing are in vitro cell culture techniques and in silico computer simulation. (wikipedia.org)
  • The testing of cosmetic products directly onto an animal can be minimized or eliminated by the use of in vitro cell growth and development. (wikipedia.org)
  • Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. (nature.com)
  • Gene therapy has been utilized successfully to repair or inactivate mutations in animal models of monogenic human diseases 19 as well as human in vitro cells 12 . (nature.com)
  • The stem cell field witnessed a genuine breakthrough when a combination of solely four transcription factors ( Oct3 / 4 , Sox2 , Klf4 and c-Myc, OSKM ) proved enough to revert, in vitro, the differentiated status of a variety of cell types back to pluripotency, giving rise to so-called induced pluripotent stem (iPS) cells. (springer.com)
  • Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)
  • The in vitro effects of MSC-Exo on immune cell migration and responder T cell proliferation were examined by chemotactic assays and lymphocyte proliferation assays, respectively. (nature.com)
  • In vitro models of human hair follicle-like tissue could be fundamental tools to better understand hair follicle morphogenesis and hair drug screening. (researchgate.net)
  • The neuronal cells expressed numerous genes associated with sub-regions within the brain thus suggesting the usefulness of our in-vitro model. (biomedcentral.com)
  • Therefore, the implantation of human cells reprogrammed in vitro represents an alternative strategy [ 16 - 19 ]. (thno.org)
  • Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
  • On the other hand, even if 3D aggregate culture methods are able to recapitulate in vitro the cell-extracellular matrix interactions properly observed in vivo, and the synthetic/natural matrix and scaffolds have biochemical and mechanical properties, in order to mimic the native extracellular matrix, both of these systems possesses some limitations and some methodological improvements are needed. (razavihospital.ir)
  • Results: The processes by which re-aggregated adult single cell types or adult and embryonic explanted tissues are able to recapitulate embryogenesis in vitro, when cultured in adhesion or embedded in 3D gels, is not surprising and is clearly under the control of a reminiscent cellular memory which recapitulates early developmental stages. (razavihospital.ir)
  • Moscona A. The Development in Vitro of Chimeric Aggregates of Dissociated Embryonic Chick and Mouse Cells. (razavihospital.ir)
  • 24 , 25 DPCs have been derived from pluripotent SCs in an attempt to study their potential for hair regeneration in vitro and in vivo. (benitonovas.com)
  • In addition, we found that in the presence of LIF, MDLS could replace feeder cells to maintain the undifferentiated state of OG2-mES cells (Oct4-GFP reporter gene mouse embryonic stem cell line), and the MDLS-expanded OG2-mES cells showed an elevated expression levels of pluripotency markers in vitro. (biomedcentral.com)
  • However, one of the bottlenecks in the stem cell therapy is that stem cells tend to differentiate into other types of cells during culture in vitro. (biomedcentral.com)
  • Therefore, finding a way to effectively maintain the pluripotency of stem cells cultured in vitro is important for the application of stem cells. (biomedcentral.com)
  • However, insufficient number of cells and lack of regenerative properties during in vitro expansion still limit the clinical applicability of MSC therapies. (biomedcentral.com)
  • The population of MSC in human tissues is insufficient, and obtaining a high cellular yield and maintaining biological capacity during in vitro expansion remains challenging [ 4 ]. (biomedcentral.com)
IPSCs7
  • Infection of iPSCs with lentiviral inserts is a highly efficient process since stem cells grow quickly, remain undifferentiated in specific cell culture conditions and can establish fully infected clones within 2-3 passages. (nature.com)
  • It is well established that somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) using a cocktail of transcription factors called Yamanaka's factors (YFs) (OCT4, SOX2, KLF4, and MYC). (bvsalud.org)
  • To investigate underlying disease mechanisms, induced pluripotent stem cells (iPSCs) allow the generation of patient-derived neuronal cells in a dish. (biomedcentral.com)
  • The AD-iPSCs were differentiated into neuronal cells, in order to generate disease-specific protein association networks modeling the molecular pathology on the transcriptome level of AD, to analyse the reflection of the disease phenotype in gene expression in AD-iPS neuronal cells, in particular in the ubiquitin-proteasome system (UPS), and to address expression of typical AD proteins. (biomedcentral.com)
  • We detected the expression of p-tau and GSK3B, a physiological kinase of tau, in neuronal cells derived from AD-iPSCs. (biomedcentral.com)
  • Treatment of neuronal cells differentiated from AD-iPSCs with an inhibitor of γ-secretase resulted in the down-regulation of p-tau. (biomedcentral.com)
  • Micha's lab collaborates closely within the science faculty on stem cell organoid models for drug research, toxicity, replacement of animal models, and with the Leiden University Medical Center (LUMC) on stem cell immunology and manufacturing (GMP) of iPSCs for clinical purposes. (universiteitleiden.nl)
Mouse embryonic3
Regenerative13
Vivo4
  • The links of in vivo reprogramming to pluripotency with tumorigenesis and teratoma formation, and the cross-talk with cellular senesce and tissue injury are also discussed. (springer.com)
  • One of the kinases that phosphorylates tau in-vivo is glycogen synthase kinase-3β (GSK3B), which is widely expressed in all tissues with elevated expression in developing brains [ 13 ]. (biomedcentral.com)
  • 2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
  • In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
Papilla cells4
  • Hair regeneration using hair follicle stem cells (HFSCs) and dermal papilla cells is a promising approach for the treatment of alopecia. (researchgate.net)
  • Dermal papilla cells (DPCs) play critical roles in hair follicle development, but the underlying mechanisms that contribute to hair regeneration have yet to be fully elucidated, particularly in terms of alterations in androgenetic alopecia patients. (researchgate.net)
  • Biologic factors that include signaling from stem cells, dermal papilla cells, and platelet-rich plasma are some of the current therapeutic agents being studied for hair restoration with milder side effects. (benitonovas.com)
  • 17 Other signals can stimulate dermal papilla cells (DPCs) that promote SC proliferation in the HF. (benitonovas.com)
Difficult to reprogram1
  • Our results have practical value in improving the efficiency of pluripotent stem cell derivation from "difficult to reprogram" somatic cells, in addition to providing some insights into the mechanisms of liver carcinogenesis in chronic hepatitis B. To conclude, HBx improves the reprogramming efficiency of YFs. (bvsalud.org)
Regeneration6
  • She was awarded with a postdoc fellow from the Ministry of Science at the Technical University of Catalonia in Biomaterials for bone tissue regeneration and since 2010 has been professor at this university. (ibecbarcelona.eu)
  • During prenatal development and postnatal cyclic hair regeneration, hair follicle morphogenesis is triggered by reciprocal interactions and the organization of the epithelial and mesenchymal cell po. (researchgate.net)
  • 11 , 12 With the emergence of stem cells (SCs), many mechanisms that lead to tissue regeneration have been discovered. (benitonovas.com)
  • With improved understanding of tissue healing and regeneration, stem cell Prolotherapy is gaining significant clinical importance and potential. (journalofprolotherapy.com)
  • Following the expansion of stem cell therapy using autologous MSC for tissue regeneration [ 3 ]. (biomedcentral.com)
  • The insights gleaned from this work will have wide-reaching implications in the area of tissue regeneration and have the potential to transform the lives of patients who have suffered major burns and trauma. (regenerativemedicine.net)
Progenitor cells2
  • Intradermal adipocyte progenitor cells are activated toward the end of the telogen phase to proliferate and differentiate into mature adipocytes. (jcadonline.com)
  • Mesenchymal stem cells are pluripotent progenitor cells that have the capacity to differentiate into several mesenchymal tissues: osteoblasts, chondrocytes and adipocytes. (3hbiomedical.com)
Differentiate2
  • The main property that sets ESC apart from other cells is their pluripotent nature, meaning they can give rise/differentiate to cells of the three primary germ layers (mesoderm, endoderm and ectoderm) [ 13 ]. (biomedcentral.com)
  • Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
20231
  • Ugur E., Porte A. de la, Qin W.H., Bultmann S., Ivanova A., Drukker M.E., Mann M., Wierer M. & Leonhardt H. (2023), Comprehensive chromatin proteomics resolves functional phases of pluripotency and identifies changes in regulatory components, Nucleic Acids Research 51(6): 2671-2690. (universiteitleiden.nl)
Proliferation2
  • Hepatitis B virus x (HBx) is a multifunctional protein coded by the Hepatitis B virus that is involved in various cellular processes such as proliferation, cell survival/apoptosis, and histone methylation. (bvsalud.org)
  • However, no inhibitory effect of MSC-Exo on IRBP-specific T cell proliferation was observed. (nature.com)
Autologous6
  • The aim of this paper is to discuss the general overview of the quality assurance and quality control implemented in the manufacturing of cell and tissue product, with emphasis on our experience in the manufacturing of MyDerm ® , an autologous bilayered human skin substitute. (biomedcentral.com)
  • The adult stem cells used were autologous and were extracted from infraumbilical adipose tissue prior to abdominoplasty. (rbcp.org.br)
  • Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
  • Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)
  • Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
  • 1, 2 Recently, Prolotherapists have begun to utilize the potential of autologous adipose (fat)-derived stem/stromal cells (AD-SC) within non-manipulated fat graft scaffolding, combined with high-density PRP concentrates (HD-PRP) to provide a potent biological therapeutic combination. (journalofprolotherapy.com)
Epithelial2
  • Alveolar type II (ATII) epithelial cells synthesize, secrete and recycle all components of surfactant and dysfunction in surfactant metabolism can result in a variety of pediatric lung diseases including respiratory distress syndrome and interstitial lung disease 3 . (nature.com)
  • ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
Fibroblast4
  • Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (springer.com)
  • Integrated spatial multiomics reveals fibroblast fate during tissue repair. (stanford.edu)
  • We employ a stented wound model that recapitulates human tissue repair kinetics and multiple Rainbow transgenic lines to precisely track fibroblast fate during the physiologic response to skin injury. (stanford.edu)
  • Through integrated analysis of single cell chromatin landscapes and gene expression states, coupled with spatial transcriptomic profiling, we are able to impute fibroblast epigenomes with temporospatial resolution. (stanford.edu)
Reprogramming of somatic cells1
  • In addition, its ability to function as a pioneer factor enables the reprogramming of somatic cells back into a pluripotent state. (bvsalud.org)
Pluripotency6
  • To gain pluripotency, the reprogramming process typically involves transfecting adult somatic cells with certain pluripotency markers. (biomedcentral.com)
  • AKT/PKB is a kinase crucial for pluripotency maintenance in pluripotent stem cells. (bvsalud.org)
  • Molecular cues for pluripotency, genetic reprogramming of adult stem cells, and widely multipotent adult cells. (razavihospital.ir)
  • Octamer-binding transcription factor-4 (Oct4), a transcription factor belongs to the POU transcription factor family Class V, is fundamental for maintaining self-renewal ability and pluripotency of stem cells. (biomedcentral.com)
  • Colony formation assay, sphere-forming ability assay, alkaline phosphatase (AP) activity assay and teratoma-formation assay were used to assess the role of modaline sulfate (MDLS) in promoting self-renewal and reinforcing pluripotency of P19 cells. (biomedcentral.com)
  • These findings demonstrated, for the first time, that MDLS could maintain self-renewal and pluripotency of stem cells. (biomedcentral.com)
ESCs1
Adipocytes2
  • In the past decade, better understanding of the cellular mechanisms responsible for successful soft tissue augmentation has been reported, focusing on the plentiful undifferentiated stromal elements rather than the survival of mature adipocytes. (journalofprolotherapy.com)
  • 26,27 Dermal adipocytes are organized in small clusters under the reticular dermis and interact with the hair follicular cells regulating the hair cycle. (jcadonline.com)
Platelet-rich plasma1
  • In the 1990's platelet rich plasma (PRP) gained acceptance in many surgical circles, and in the 2000's, Prolotherapists and other physicians in the orthopedic and sports medicine field, began using high-density PRP concentrates (HD-PRP), defined as a minimum of 4 times patient baseline platelet levels, to stimulate musculoskeletal connective tissue repair. (journalofprolotherapy.com)
Blood vessels1
  • 27 Together, dermal blood vessels and DPCs orchestrate a suitable microenvironment for the growth and survival of HFSCs. (benitonovas.com)
Connective tissue4
  • Prolotherapists have known since the 1930's that a solution as innocent as dextrose, used as an irritant and properly placed, stimulates injured musculoskeletal connective tissue to heal, often dramatically. (journalofprolotherapy.com)
  • 3 In veterinary medicine, AD-SC's have been utilized effectively for over ten years in the treatment of osteoarthritic joints 4, 5 and connective tissue injuries, showing an over 80% success rate in blinded placebo controlled canine clinical trials. (journalofprolotherapy.com)
  • Adipose tissue is a loose connective tissue composed of cells supported by an intracellular matrix as well as by vascular, lymphatic, and neural networks. (jcadonline.com)
  • Type IV collagen forms the basis of basement membranes, which are thin layers of connective tissue that separate and support various cell layers. (intactone.com)
Found in bone marrow2
Embryonic stem2
Somatic cells3
  • Induced pluripotent stem cells (iPS) can be generated from various somatic cells and can subsequently be differentiated to multiple cell types of the body. (biomedcentral.com)
  • The stemness inducing properties of HBx could also facilitate the generation of pluripotent stem cells from somatic cells. (bvsalud.org)
  • Recent work has demonstrated that the epigenome is already rejuvenated by the maturation phase of somatic cell reprogramming, which suggests full reprogramming is not required to reverse ageing of somatic cells. (elifesciences.org)
Adipose derived stem1
IPSC1
  • Kaynaklarına göre (a) embriyonik (ESC), (b) yetişkin ve (c) indüklenmiş pluripotent kök hücreler (IPSC) olarak sınıflandırılırlar (2,3). (adaveteriner.com)
Organoids1
  • We also show the presence of normal lamellar bodies and the secretion of surfactant into the cell culture medium in the organoids of lentiviral infected cells. (nature.com)
Severe4
  • Shih CC, Forman SJ, Chu P, Slovak M. Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice. (razavihospital.ir)
  • Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)
  • Prolotherapy is a proven technique for resolving musculoskeletal pain, but can have limitations if tissue damage is too severe. (journalofprolotherapy.com)
  • Blistering may be severe and granulation tissue can form on the skin around the oral and nasal cavities, fingers and toes, and internally around the upper airway. (nih.gov)
Neonatal1
Centre1
Mechanisms5
  • Micha's interest lies in discovering mechanisms that regulate the formation of the embryonic tissues using iPS cells, in order to create novel models for human disease and drug development with emphasis on neurodegeneration. (universiteitleiden.nl)
  • The lab focuses on multifaceted regulation by transcription and epigenetic factors, lncRNAs, membraneless organelles, mechanisms of self-renewal, and extensively uses organoid technology, single cell RNA sequencing, flow cytometry, and CRISPR-Cas9 genetic engineering approaches. (universiteitleiden.nl)
  • Fundamental questions in organogenesis are related to the identification of a simplified model which is able to mimic the molecular mechanisms involved in pattern organization and cell fate determination as well. (razavihospital.ir)
  • However, most of the mechanisms exerted by these factors in hair restoration are still being researched. (benitonovas.com)
  • Although an early study, our research sheds further light on the complex architecture of the skin and the mechanisms triggered in response to skin wounds. (regenerativemedicine.net)
Bone5
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
  • Despite improved surgical techniques, the tendon-to-bone healing rate is unsatisfactory due to difficulties in restoring the delicate transitional tissue between bone and tendon. (wjgnet.com)
  • The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
  • Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
  • Langerhans cell histiocytosis (LCH) can be localized and manifest as pain or may even be asymptomatic, as is the case in isolated bone lesions. (medscape.com)
Hair follicles2
  • These cells, surrounding the hair follicles, secrete signaling molecules that control the progression of the hair cycle. (jcadonline.com)
  • 22 The underlying mechanism is believed to be a higher concentration of dihydrotestosterone (DHT) to androgen-sensitive hair follicles, causing thinning of the dermal papillae and shortening of the anagen phase. (jcadonline.com)
Androgenetic alopecia1
  • In this study, we demonstrated that hypoxia-inducible factor-1a (HIF-1a) is suppressed in scalp tissues of androgenetic alopecia patients and potentially associated with hair fol. (researchgate.net)
20222
  • Drukker M.E. (18 November 2022), Cell pharmacy (Inaugural address. (universiteitleiden.nl)
  • Matheus F., Raveh T., Oro A.E., Wernig M. & Drukker M.E. (2022), Is hypoimmunogenic stem cell therapy safe in times of pandemics? (universiteitleiden.nl)
Biology4
  • They can be used to study cellular and metabolic biology without the use of an animal model or fetal tissue. (nature.com)
  • Improved understanding of the pathology of histiocytic disorders requires knowledge of the origins, biology, and physiology of the cells involved. (medscape.com)
  • After completion of his postdoctoral training with Dr Roger Brent in the Department of Molecular Biology, Massachusetts General Hospital and in the Department of Genetics, Harvard Medical School in Boston, he moved to the Telethon Institute of Genetics and Medicine (TIGEM) in Milan in 1998 to lead a research group. (agbt.org)
  • Michael Schatz, Bloomberg Distinguished Professor of Computer Science and Biology at Johns Hopkins University, is among the world's foremost experts in solving computational problems in genomics research. (agbt.org)
Crucial1
  • It plays a crucial role in providing strength, structure, and elasticity to various tissues and organs. (intactone.com)
Placental1
  • Trophoblasts are multifunctional placental cells critical in pregnancy maintenance and communication between the fetus and mother [ 12 ]. (biomedcentral.com)
Microenvironment1
  • Through them, the cells modify their microenvironment and the behavior of neighboring cells. (frontiersin.org)
Multipotent cells1
  • Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
Methods3
  • However, even though cell or tissue culture methods may reduce the number of experiments performed on intact animals, the maintenance of cells in culture normally requires the use of animal-derived serum. (wikipedia.org)
  • Several tissue culture methods that measure the rate of chemical absorption by the skin have been approved by the OECD. (wikipedia.org)
  • Advances in 3D non-destructive, non-invasive analysis methods and improvements in the multi-scale techniques and bioreactors to obtain test and culture 3D cell aggregates have been made. (razavihospital.ir)
Cardiac3
  • Our results highlight the remarkablepotential use of BC-Ppy scaffolds as a cardiac patch in tissue regenerativetherapies. (ibecbarcelona.eu)
  • Nano-thin and nano-porous membranes and heart like electric stimulus were used in the cardiac-mimetic cell-culture system. (thno.org)
  • An immunologically identical cardiac tissue can be produced by direct reprogramming from the patients' tissue without the potential risk of tumorigenesis that occurs with induced pluripotent stem cells [ 2 ] and the ethical issue using embryonic stem cells. (thno.org)
Adherent1
Clinical9
  • In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
  • The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
  • In recent years, the development in cell therapy and stem cell research has led scientist to engage in the production of cells and tissue product that is of clinical grade. (biomedcentral.com)
  • Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
  • Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
  • In this review, we discuss the potential influence of stromal vascular fraction from adipose tissue in the management of alopecia as well as its involvement in preclinical and clinical trials. (jcadonline.com)
Therapeutic1
  • It is highly recommended to perform cell manufacturing for therapeutic use in a well-designed GMP-compliant cleanroom facility. (biomedcentral.com)
Angiogenesis1
  • CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. (thermofisher.com)
Implantation2
  • Skin healing results after implantation of adult stem cells derived from adipose tissue were satisfactory. (rbcp.org.br)
  • After implantation, self-renewal and proliferative cytotrophoblasts appear in the early first trimester (6-9 weeks) and can help identify trophoblast stem cells (TSCs) [ 13 ]. (biomedcentral.com)
Extracellular vesicles3
  • The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. (frontiersin.org)
  • The exosome vesicles are a type of extracellular vesicles (EV), which are defined as lipid-bilayer spheroid structures, without replicating capacity, that are released from cells, including both prokaryotes and eukaryotes. (frontiersin.org)
  • A recent study supports the pivotal role of trophoblast exosomes in that extracellular vesicles (EVs) derived from TSCs promote fertility by regulating endometrial cell receptivity [ 17 ]. (biomedcentral.com)
Induction1
  • Additionally, we concluded that this induction takes place in different pluripotent contexts but not in terminally differentiated cells. (bvsalud.org)
Vascular smooth2
  • The treatment attenuated the weight loss, improved the survival rate by approximately 25%, suppressed epidermal thinning and dermal fat loss, improved degeneration of the vascular smooth muscle cells of the aortic arch, attenuated the development of bradycardia and increased the vitality of the mice. (bioethicsobservatory.org)
  • were the first to assess nanoparticle-like exosomes released from trophoblasts and determine the functional activity of trophoblast exosomes in human uterine spiral artery remodeling by inducing vascular smooth muscle cell migration for successful pregnancy [ 16 ]. (biomedcentral.com)
Squamous Cell Carc2
  • RHCG Suppresses Tumorigenicity and Metastasis in Esophageal Squamous Cell Carcinoma via Inhibiting NF-kappaB Signaling and MMP1 Expression. (cusabio.com)
  • in the second decade of life, surveillance for squamous cell carcinoma is appropriate. (nih.gov)
Genes3
  • Transcriptome analysis of AD-iPS derived neuronal cells revealed significant changes in the expression of genes associated with AD and with the constitutive as well as the inducible subunits of the proteasome complex. (biomedcentral.com)
  • Genes Cells. (razavihospital.ir)
  • Immunofluorescence, RT-PCR, and western blotting were used to measure expression changes of stem-related genes and activation of related signaling pathways. (biomedcentral.com)
Wound3
  • These cells were implanted into the surgical wound dermis in the suprapubic region before skin synthesis. (rbcp.org.br)
  • Here, we present a multimodal -omics platform for the comprehensive study of cell populations in complex tissue, which has allowed us to characterize the cells involved in wound healing across both time and space. (stanford.edu)
  • Collagen is involved in the wound-healing process, where it helps to form a scaffold for new tissue growth and repair. (intactone.com)
Liver2