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Induced Pluripotent Stem CellsPluripotent Stem CellsStem CellsEmbryonic Stem CellsNuclear ReprogrammingCell DifferentiationStem Cell TransplantationHematopoietic Stem CellsOctamer Transcription Factor-3Adult Stem CellsCell DedifferentiationRegenerative MedicineCell Culture TechniquesSOXB1 Transcription FactorsCell LineageNeural Stem CellsEmbryoid BodiesCells, CulturedMultipotent Stem CellsMesenchymal Stromal CellsStem Cell ResearchHematopoietic Stem Cell TransplantationStem Cell NicheTeratomaGerm LayersFeeder CellsStage-Specific Embryonic AntigensCell ProliferationCell- and Tissue-Based TherapyFibroblastsRegenerationKruppel-Like Transcription FactorsMesenchymal Stem Cell TransplantationCell LineCell SeparationGerm CellsEmbryo, MammalianLentivirusBone Marrow CellsMice, SCIDStem Cell FactorFlow CytometryNeoplastic Stem CellsTissue EngineeringGene Expression ProfilingEpigenesis, GeneticMyocytes, CardiacGene Expression Regulation, DevelopmentalHematopoiesisHomeodomain ProteinsCell TransplantationBiological MarkersCoculture TechniquesMice, Inbred C57BLReverse Transcriptase Polymerase Chain ReactionNeuronsModels, BiologicalTranscription FactorsPhenotypeTotipotent Stem CellsGene Expression RegulationTransduction, GeneticFetal Stem CellsProto-Oncogene Proteins c-mycPlant StemsKaryotypingGenetic VectorsSignal TransductionGreen Fluorescent ProteinsMice, Inbred NODImmunohistochemistryTransgenesMice, TransgenicChimeraAntigens, CD34Gene ExpressionCell TransdifferentiationEndodermHematopoietic Stem Cell MobilizationColony-Forming Units AssayActivinsEmbryonal Carcinoma Stem CellsLeukemia Inhibitory FactorBone Morphogenetic Protein 4Embryo ResearchPlanariansNestinX Chromosome InactivationSpermatogoniaCell EngineeringCell ShapeCell SurvivalCell DivisionDNA MethylationTransplantation, AutologousMicroRNAsBlastocyst Inner Cell MassNeurogenesisKaryotypeAntigens, Differentiation
Called induced pluriIPSCsDifferentiation of human pluripotent cellsIPScNeuronsFound in bone marrowMesenchymal stemOrganoidsFibroblastsRegenerative medicineReprogrammingBone marrowHiPSCsTransplantationPhenotypesBiologyProgenitor cellsHematopoietic stemAbility to differentiateVitroGenesLineagesAutologousParkinson'sNeural cellsGenerateDopaminergic progenitorsTherapiesSelf-renewalTherapeuticHESCsMorphologyProliferationPluripotencyNeuronalCapabilityESCsEmbryonic stem cellFunctionalNucleiGenomicTissuesGeneticHuntington'sTissueHarvard Stem Cell InstituteMiceCharacteristicsResearchersBiologicalAdult stemImmatureCardiomyocytesTypesEmbryoBrainCellularTherapyMyeloidResearchScientistsHuman stem-cellPathwaysPhysiology
Called induced pluri1
IPSCs22
  • The discovery in 2006 that human and mouse fibroblasts could be reprogrammed to generate induced pluripotent stem cells (iPSCs) with qualities remarkably similar to embryonic stem cells has created a valuable new source of pluripotent cells for drug discovery, cell therapy, and basic research. (thermofisher.com)
  • Our products have been an integral part of induced pluripotent stem cell research from the initial discovery of iPSCs to current breakthroughs. (thermofisher.com)
  • To create iPSCs, scientists genetically reprogram the adult stem cells so they behave like embryonic stem cells. (healthline.com)
  • In addition to human iPSCs, it can be used to replate culture of primary neurons or to resuspend them for fact sorting or single cell sequencing. (jove.com)
  • Differentiated human induced pluripotent stem cells (iPSCs) following differentiation protocol. (cellimagelibrary.org)
  • Regenerated airway and alveolar cells derived from human iPSCs express proteins that match human respiratory cell biomarkers and play an important role in pathogenesis, such as ACE2 receptor protein in SARS-CoV-2 infections. (genengnews.com)
  • In 2012 Shinya Yamanaka and Sir John Gurdon were awarded the Nobel Prize for directly generating induced pluripotent stem cells (iPSCs) from somatic cells by introducing four genes, now collectively called the Yamanaka factors (Myc, Oct3/4, Sox2 and Klf4). (genengnews.com)
  • Regenerative medicine aims to derive specialized cell types from iPSCs that can provide disease models for therapeutic development and can be used to transplant, replace, engineer or regenerate diseased cells. (genengnews.com)
  • Specifically, Dolmetsch wanted to differentiate induced pluripotent stem cells (iPSCs) into neurons to study a related genetic disorder called Timothy syndrome. (the-scientist.com)
  • Not long after joining the lab, Pasca started to develop ways to differentiate neurons from iPSCs and derived them from individuals with and without Timothy syndrome. (the-scientist.com)
  • By adding three genes, they converted those cells into induced pluripotent stem cells (iPSCs), which have the capability to differentiate into any cell types in the body. (scienceblog.com)
  • Starting with procedures other research teams had previously worked out to convert iPSCs into keratinocytes, Xu's team demonstrated that by carefully controlling the timing of the growth factors the cells received, they could force the iPSCs to generate large numbers of epithelial stem cells. (scienceblog.com)
  • In the Xu study, the team's protocol succeeded in turning over 25% of the iPSCs into epithelial stem cells in 18 days. (scienceblog.com)
  • What's more, the process Xu used to create iPSCs involves genetic modification of human cells with genes encoding oncogenic proteins and so needs more refinement. (scienceblog.com)
  • B ) Morphology of induced pluripotent stem cells (iPSC) colonies, ( C ) proliferation rate of iPSCs, and ( D ) photoreceptor primary cilium of organoids were compared between WT and rd16 . (elifesciences.org)
  • To understand the clinical impact of RBM20 mutations, we have generated induced pluripotent stem cells (iPSCs) from adult patients and healthy controls. (stanford.edu)
  • Therefore, mMSCs were reprogrammed into induced pluripotent stem cells (iPSCs) by non-integrative chromosomal technologies and differentiated into corresponding iP-MSCs. (uni-muenchen.de)
  • From this synthetic genome some relevant parts were biologically synthesised and inserted into human induced pluripotent stem cells (also known as iPS cells or iPSCs), which were then differentiated into beating heart cells. (we-make-money-not-art.com)
  • Although remarkable progress in induced pluripotent stem cell (iPSC)-derived cell therapies has been made, very little is known about the role of mitochondria and the mechanisms involved in somatic cell reprogramming into iPSC and directed reprogramming of iPSCs in terminally differentiated cells. (bvsalud.org)
  • Skin cells are taken from a patient, reprogrammed to induced pluripotent stem cells (iPSCs), differentiated into dopaminergic progenitors, and put back in the patient. (lu.se)
  • These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
  • The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)
Differentiation of human pluripotent cells1
  • Using appropriate media conditions for differentiation of human pluripotent cells to CD34+ vascular progenitor cells, we show that temporary expression of pluripotent transcription factors and treatment with chemically-defined media, will induce differentiation of human fibroblasts to CD34+ vascular progenitor cells. (nih.gov)
IPSc15
  • Our complete portfolio of stem cell research products and services provides optimized tools for each step of the stem cell workflow: from reprogramming somatic cells to iPSC expansion, validation, and differentiation. (thermofisher.com)
  • Reprogramming of somatic cells for iPSC generation can be accomplished with a number of technologies. (thermofisher.com)
  • Analyze stem cells and their derivatives using a wide range of cellular and molecular characterization methods, as validation is critical in iPSC research. (thermofisher.com)
  • Using five control induced pluripotent stem cell (iPSC) lines and seven iPSC lines generated from fAD patients, we investigated the effects of mutations on the Aβ secretome in human neurons generated in 2D and 3D. (nature.com)
  • Induced pluripotent stem cells represent one of the most promising research advances within the past decade, making this a valuable report for both executives and investors to use to optimally position themselves to sell iPSC products. (prnewswire.com)
  • Submitter supplied) We report a novel technique to reprogram human fibroblasts into endothelial and smooth muscle cells using partial iPSC reprogramming and chemically defined media. (nih.gov)
  • Comparison of the gene expression patterns of the human iPSC-derived epithelial stem cells with epithelial stem cells obtained from human hair follicles showed that the team had succeeded in producing the cells they set out to make in the first place. (scienceblog.com)
  • That said, iPSC-derived epithelial stem cells are not yet ready for use in human subjects, Xu adds. (scienceblog.com)
  • Xu and his team mixed iPSC-derived EpSCs and mouse dermal cells to generate hair follicles to achieve the growth of the follicles. (scienceblog.com)
  • NC-MSCs are mesenchymal stem cells derived from a single donor's fibroblasts that have been dedifferentiated into an induced pluripotent stem cell (iPSC) master cell bank, thereby avoiding the need to source additional donor cells. (prnewswire.com)
  • A ) Morphology of Nrl -GFP wild-type (WT) and rd16 retinal organoids differentiated from mouse-induced pluripotent stem cells (iPSC) at various differentiation time points. (elifesciences.org)
  • Differentiation of autologous iPSC into terminally differentiated ß-like cells requires further metabolic adaptation. (bvsalud.org)
  • Our most recent initiative is having established a platform for induced pluripotent stem cells (iPSC). (lu.se)
  • We procure skin biopsies from patients, reprogram fibroblasts from these into stem cells, i.e., iPSC, which are then differentiated into insulin-producing beta-like cells. (lu.se)
  • Chad and I have both been principal investigators on the induced pluripotent stem cell (IPSC) grant. (medscape.com)
Neurons19
  • Here, the stem cells are shown expressing various markers and differentiating into neurons. (livescience.com)
  • Reprogrammed cells could then develop into a multitude of cell types, including the neurons found in the brain and spinal cord. (livescience.com)
  • This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. (jove.com)
  • This protocol facilitates the use of human pluripotent stem cell-derived neurons for high content screening applications. (jove.com)
  • Human pluripotent stem cell-derived neurons are increasingly relevant in the areas of basic research, drug development, and degenerative medicine. (jove.com)
  • Start by gently rinsing the plate of differentiated neurons with PBS. (jove.com)
  • Moreover, tranylcypromine treatment affects neurons and astrocytes, which impairs cell density and arrangement. (frontiersin.org)
  • Two years later, the team reported that neurons derived from reprogrammed skin cells of individuals with the disorder had defects in calcium signaling, a result that made the cover of Nature Medicine (17:1657-62, 2011). (the-scientist.com)
  • Now, for the first time, Dr. Su-Chun Zhang and coworkers at the University of Wisconsin-Madison have successfully generated neurons from reprogrammed nonhuman primate cells, transplanted them back into the same animal's brain, and seen them successfully and cleanly integrate into the local tissue. (sens.org)
  • Here we have used the induced pluripotent stem cell technology to produce patient-specific terminally differentiated GABA-ergic medium spiny neurons modeling a juvenile form of HD (HD76). (frontiersin.org)
  • The most vulnerable cells in HD are the striatal medium spiny neurons ( Vonsattel and DiFiglia, 1998 ). (frontiersin.org)
  • Zhang's group, led by researcher Baoyang Hu, found that the induced cells differentiate into progenitor neural cells and further into the different kinds of functional neurons that make up the brain. (scienceblog.com)
  • In the present study, to recapitulate the pathologies of these disorders in vitro , we established in vitro models by differentiating mature neurons from human induced pluripotent stem cells (hiPSCs) derived from BP and SCZ patient with contributive copy number variations, as follows: two BP patients with PCDH15 deletion and one SCZ patient with RELN deletion. (nih.gov)
  • Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
  • First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
  • Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
  • This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
  • The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)
  • Stem Derived Dopamine Neurons: Will They Replace DBS as the Leading Neurosurgical Treatment for Parkinson's Disease? (lu.se)
Found in bone marrow2
Mesenchymal stem4
Organoids8
  • She worked with a multi-institution group based at Boston University that was the first to generate thyroid cell progenitors and thyroid follicular organoids from pluripotent stem cells (PSCs) in mice, and thyroid cell progenitors from induced PSCs in humans. (wikipedia.org)
  • Recent breakthroughs in human pluripotent stem cell-derived cerebral organoids provide a valuable platform for investigating the human brain after different drugs treatments and for understanding the complex genetic background to human pathology. (frontiersin.org)
  • Here, we identified tranylcypromine, which is used to treat refractory depression, caused human-induced pluripotent stem cell-derived brain organoids neurotoxicity, leading to decreased proliferation activity and apoptosis induction. (frontiersin.org)
  • Recent breakthroughs in human pluripotent stem cells (hPSCs)-derived cerebral organoids offer a promising approach for investigating the mystery of human brain ( 19 ). (frontiersin.org)
  • The Stanford University professor helped develop a technique to grow brain organoids from induced pluripotent stem cells. (the-scientist.com)
  • One-way ANOVA was performed to compare the %GFP+ cells and viability of organoids from four different cell lines. (elifesciences.org)
  • Phenotypes of retinal organoids differentiated from induced pluripotent stem cells of Nrl -GFP rd16 mice. (elifesciences.org)
  • B ) Immunostaining of rod cell marker rhodopsin (RHO, green) and cone cell marker S-opsin (OPN1SW, red) in wild-type (WT) and rd16 organoids treated with non-toxic positive hits (B01-B05). (elifesciences.org)
Fibroblasts6
Regenerative medicine7
Reprogramming11
  • We offer choices in integration-free reprogramming technologies and services that fit your research and translational needs including our Cell Therapy Systems (CTS) Products, which are designed for clinical and translational research applications. (thermofisher.com)
  • Which stem cell reprogramming technology is right for you? (thermofisher.com)
  • Successfully reprogramming induced pluripotent stem cells so they behave like the cells they are meant to replace means that samples of the mimicked cells must be present for comparison. (livescience.com)
  • The cells, called NoveCite Cells or NC-MSCs, are made by Novellus, Inc. ("Novellus"), a Cambridge-based biotechnology company, using its patented mRNA-based cell-reprogramming process. (prnewswire.com)
  • Reprogramming" of adult differentiated cells into pluripotent stem cells is an exciting method in biology that holds enormous promise for rejuvenation biotechnology. (sens.org)
  • Olariu V, Manesso E, commitment and Peterson C. 2017 A deterministic method for estimating free energy genetic network reprogramming paths landscapes with applications to cell commitment and reprogramming paths. (lu.se)
  • those of cell reprogramming, thereby avoiding exhaustive trial- energy landscape, deterministic models, and-error simulations with rate equations for different stem cell commitment, reprogramming parameter sets. (lu.se)
  • We explore the method on three circuits for haematopoiesis and embryonic stem cell development for commitment and reprogramming scenarios and illustrate how the method can be used to determine sequential steps for onsets of external factors, essential for efficient reprogramming. (lu.se)
  • To deal with these challenges, the application of stem cells, especially cellular reprogramming of somatic cells to human pluripotent stem cells, also known as induced pluripotent stem cells, has been able to promise a new chapter in the modeling of Parkinson's disease. (bvsalud.org)
  • Also, since these cells are obtained from the reprogramming of somatic cells in the patient's body, they maintain the patient's genetic content, which can play an important role in increasing the quality of disease modeling and the validity of the results of laboratory studies. (bvsalud.org)
  • Mitochondria are the powerhouse of the cell and dynamically control fundamental biological processes including cell reprogramming, pluripotency, and lineage specification. (bvsalud.org)
Bone marrow9
HiPSCs5
  • However, despite the common ability of hiPSCs and hESCs to differentiate into all 3 germ layers, their functional equivalence at the single cell level remains to be demonstrated. (jci.org)
  • Here, we used single cell analysis to resolve the gene expression profiles of 362 hiPSCs and hESCs for an array of 42 genes that characterize the pluripotent and differentiated states. (jci.org)
  • Comparison between single hESCs and single hiPSCs revealed markedly more heterogeneity in gene expression levels in the hiPSCs, suggesting that hiPSCs occupy an alternate, less stable pluripotent state. (jci.org)
  • We aim to develop a cell culture platform to automatically differentiate human induced pluripotent stem cells (hiPSCs) for lab-on-a chip applications. (dim-elicit.fr)
  • He notes the science behind adult stem cells that can be "reprogrammed," called human induced pluripotent stem cells (hiPSCs), is still in its infancy, having become widely available in 2007. (sciencedaily.com)
Transplantation2
  • My projects investigated whether patient-derived cells are more prone to develop disease phenotypes and what can be done to protect these cells after transplantation," explained Fredrik. (lu.se)
  • Treatment includes induction chemotherapy to achieve remission and postremission chemotherapy (with or without stem cell transplantation) to avoid relapse. (msdmanuals.com)
Phenotypes1
  • However, since the patient-derived cells have a disease background it could be so that they develop disease-associated phenotypes and degenerate over time. (lu.se)
Biology3
  • We believe that the system will become a powerful tool not only in cell biology but also in other fields, including biomedical research and clinical therapy. (sciencedaily.com)
  • During a biology course, we had a guest speaker from Lund Stem Cell Center who talked about stem cells and their potential to become any type of cell in the body. (lu.se)
  • Improved understanding of the pathology of histiocytic disorders requires knowledge of the origins, biology, and physiology of the cells involved. (medscape.com)
Progenitor cells3
  • Mediates ex vivo expansion of cord blood CD34+ hematopoietic stem and progenitor cells (Huang et al. (stemcell.com)
  • Activation of OCT4 enhances ex vivo expansion of human cord blood hematopoietic stem and progenitor cells by regulating HOXB4 expression. (stemcell.com)
  • Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into forebrain neural progenitor cells. (lu.se)
Hematopoietic stem2
Ability to differentiate3
  • While the appeal of embryonic cells has been their ability to differentiate into any type of cell, there has been significant ethical, moral, and spiritual controversy surrounding their use. (prnewswire.com)
  • Pluripotent stem cells have the innate potential for unrestricted self-renewal and the ability to differentiate into any cell type in the body. (genengnews.com)
  • Mast cells are derived from pluripotent stem cells, which have the ability to differentiate into various cell types. (proprofs.com)
Vitro4
  • Owing to their manifold roles in health and disease, there have been intense efforts to synthetically generate blood vessels in vitro from human pluripotent stem cells (hPSCs). (bvsalud.org)
  • How can we specifically generate either arterial or venous endothelial cells (ECs) from hPSCs in vitro? (bvsalud.org)
  • In vitro experiments using NV virus-like particles (VLPs) directly showed NV VLP attachment to HBGA, resulting in VLP internalization into the cell ( 19 ). (cdc.gov)
  • From these critical studies, putative NV receptors were identified, and thus it was hypothesized that a successful in vitro cell culture system would most likely possess these receptors to support NV replication. (cdc.gov)
Genes6
  • To identify a set of key genes consistently altered during differentiation of hESCs regardless of differentiation conditions we have performed microarray analysis on undifferentiated hESCs (H1 and H9) and differentiated EB's and validated our results using publicly available expression array data sets. (ca.gov)
  • Intriguingly, the new study suggests the presence or absence of the genes used to reprogram skin cells to become the blank slate pluripotent cells makes no difference in terms of their capacity to differentiate. (scienceblog.com)
  • Some of the induced stem cell lines tested in the study were made using techniques that bypassed the use of genes that had been used to reprogram skin cells to become pluripotent stem cells. (scienceblog.com)
  • The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
  • En route to the patient we can also remove disease-associated genes that might compromise the transplanted cells and eventually the clinical benefit of the graft over time," described Fredrik. (lu.se)
  • The insulin-producing cells that we can make from these patients can then be used to understand the mechanisms by which these risk genes cause dysfunction of beta-cells. (lu.se)
Lineages3
  • These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
  • Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)
  • Each category of histiocytosis can be traced to reactive or neoplastic proliferation in one of these cell lineages. (medscape.com)
Autologous3
  • The focus was on evaluating the potential of using patient-specific cells (i.e., autologous grafting). (lu.se)
  • The overall aim of this thesis has been to assess the potential of autologous grafting in cell replacement therapy for PD. (lu.se)
  • Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)
Parkinson's5
  • Conditions such as Huntington's disease, Parkinson's disease, and spinal cord injuries are examples of clinical applications in which stem cells could offer benefits in halting or even reversing damage. (prnewswire.com)
  • Therefore, the procedure for modeling induced pluripotent stem cells for Parkinson's disease is explained in this chapter. (bvsalud.org)
  • For the last four years, Fredrik has explored the use of patient-specific cells in cell replacement therapy for Parkinson's disease to identify strategies to safeguard these transplanted cells from disease-related degeneration. (lu.se)
  • My Ph.D. studies revolved around cell replacement therapy for Parkinson's disease. (lu.se)
  • My thesis cover illustrates the workflow of generating patient-specific cells for cell replacement therapy for Parkinson's Disease. (lu.se)
Neural cells2
  • They transplanted human Fragile X neural cells differentiated from induced pluripotent stem cells into brains of neonatal mice and then testing for FMR1 reactivation. (fraxa.org)
  • To circumvent these obstacles, we have developed two methods for the investigation of human neural cells in culture. (lu.se)
Generate4
  • To directly differentiate to pancreatic cells, use our pancreatic progenitor kit to generate functional PDX1 + /NKX6.1 + cells that can be matured into insulin-producing beta cells. (stemcell.com)
  • But it hasn't been possible to generate sufficient number of hair-follicle-generating stem cells - until now. (scienceblog.com)
  • Dec. 16, 2021 Researchers have developed a method to generate embryo-like cell complexes from the stem cells of mice. (sciencedaily.com)
  • That means that at this point there is still some work to be done to generate ideal induced pluripotent stem cells for application. (scienceblog.com)
Dopaminergic progenitors1
  • Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
Therapies5
  • As such, this work could help lead to novel stem cell therapies and shed light on a variety of mental disorders, such as schizophrenia , autism and bipolar disorder, which may stem from problems with development, researchers say. (livescience.com)
  • Cells from corpses might play a key role in developing future stem cell therapies . (livescience.com)
  • The focus of Assoc. Prof. Elliott's laboratory is to develop pluripotent stem cell-based models of heart disease and use these models to find new therapies for heart disease. (edu.au)
  • We may never be able to choose between iPS and ES cell research because we don't know which type of cell will be best for eventual therapies. (sciencedaily.com)
  • The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)
Self-renewal6
  • Oct-4 (POU5F1) is a transcription factor that is critically involved in the self-renewal of pluripotent stem cells, and its expression is commonly used as a marker for pluripotency. (stemcell.com)
  • Discovery of consensus gene signature and intermodular connectivity defining self-renewal of human embryonic stem cells. (ca.gov)
  • Our understanding of self-renewal and differentiation capacity of human embryonic stem cells (hESCs) remains elusive on the detailed molecular mechanisms. (ca.gov)
  • Bioinformatics approach for the identification of new molecular markers defining undifferentiated hESCs, interacting partners and interconnectivity analyses may contribute to delineating molecular mechanisms of stem cell self-renewal/differentiation and can be a useful tool to identify molecular factors inducing stemness from different cell types. (ca.gov)
  • Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
  • Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)
Therapeutic2
  • A multimodal therapeutic approach involving radiotherapy is required when treating head and neck squamous cell carcinoma. (uni-muenchen.de)
  • This high throughput drug screen uses neural stem cells from Fragile X knockout mice to identify small molecules which may be therapeutic in Fragile X. (fraxa.org)
HESCs1
Morphology2
  • We demonstrated that mMSCs and iP-MSCs show similar cell characteristics in terms of morphology, clonogenic potential, differentiation, and surface phenotype. (uni-muenchen.de)
  • BC-Ppy scaffolds improved cell viability andattachment, promoting a desirable cardiomyoblast morphology. (ibecbarcelona.eu)
Proliferation6
  • Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
  • To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
  • This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
  • In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers of immature blood cells and replacement of normal marrow by malignant cells. (msdmanuals.com)
  • Malignant transformation and uncontrolled proliferation of an abnormally differentiated. (msdmanuals.com)
  • Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
Pluripotency4
  • This method is used to detect sub-genome-wide mobility changes that depend on the activity of a pluripotency-related gene in mouse embryonic stem cells. (sciencedaily.com)
  • For example, this system enabled detection of sub-genome-wide mobility changes that depend on the transcriptional activity of the Nanog gene, which encodes a key transcription factor for pluripotency, in mouse embryonic stem cells. (sciencedaily.com)
  • Transcription factors have an important role in the ability of a cell to self-renew and also differentiate into most cell types, also known as pluripotency 1 . (biolegend.com)
  • A process where fully differentiated or specialized cells revert to pluripotency or a less differentiated cell type. (bvsalud.org)
Neuronal1
  • Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)
Capability2
ESCs1
  • Molecular markers defining self-renewing pluripotent embryonic stem cells (ESCs) have been identified by relative comparisons between undifferentiated and differentiated cells. (ca.gov)
Embryonic stem cell1
  • The new Wisconsin study compared the ability of five embryonic stem cell lines with 12 induced cell lines coaxed into being using different methods. (scienceblog.com)
Functional4
  • See More Use our definitive endoderm (DE) differentiation kit to obtain functional DE cells that are capable of downstream differentiation to hepatic, intestinal, pancreatic and pulmonary cells. (stemcell.com)
  • When they mixed those cells with mouse follicular inductive dermal cells and grafted them onto the skin of immunodeficient mice, they produced functional human epidermis (the outermost layers of skin cells) and follicles structurally similar to human hair follicles. (scienceblog.com)
  • Balafkan N, Mostafavi M, Siller R, Liang KX, Sullivan G, Bindoff LA. A method for differentiating human induced pluripotent stem cells toward functional cardiomyocytes in 96-well microplates. (uib.no)
  • Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
Nuclei2
  • Cell nuclei were stained with DAPI (blue), nestin (red) and beta3-tubulin/Tuj1 (green). (cellimagelibrary.org)
  • The process that reverts CELL NUCLEI of fully differentiated somatic cells to a pluripotent or totipotent state. (bvsalud.org)
Genomic1
  • The ROLEX system is expected to be employed in studies investigating the dynamics of higher-order gene regulation via long-range genomic interactions, and for evaluating whether variability in the genomic organization of cells causes cell-to-cell heterogeneity in gene expression. (sciencedaily.com)
Tissues6
  • These stem cells come from developed organs and tissues in the body. (healthline.com)
  • The possibilities arising from these characteristics have resulted in great commercial interest, with potential applications ranging from the use of stem cells in reversal and treatment of disease, to targeted cell therapy, tissue regeneration, pharmacological testing on cell-specific tissues, and more. (prnewswire.com)
  • Studying how induced pluripotent stem cells develop into various tissues could also shed light on disorders that are due to malfunctions in development. (livescience.com)
  • These cells can be differentiated into various human tissues, including heart cells (cardiomyocytes). (stanford.edu)
  • The histiocytoses encompass a group of diverse disorders characterized by the accumulation and infiltration of variable numbers of monocytes, macrophages, and dendritic cells in the affected tissues. (medscape.com)
  • Dendritic cells display a large amount of MHC-peptide complexes at their surface and can increase the expression of costimulatory receptors and migrate to the lymph nodes, spleen, and other lymphoid tissues, where they activate specific T cells. (medscape.com)
Genetic5
  • These cells have been successfully used to treat children with blood cancers, such as leukemia, and certain genetic blood disorders. (healthline.com)
  • However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
  • In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
  • However, a new study published this week (Feb. 15) in the Proceedings of the National Academy of Sciences comparing the ability of induced cells and embryonic cells to morph into the cells of the brain has found that induced cells - even those free of the genetic factors used to program their all-purpose qualities - differentiate less efficiently and faithfully than their embryonic counterparts. (scienceblog.com)
  • It was predicted, Zhang explains, that the absence of exotic genetic factors would result in cells essentially identical to embryonic stem cells. (scienceblog.com)
Huntington's1
  • We showed how insulin deficiency in Friedreich's ataxia is likely to evolve, and that a mouse model for Huntington's disease develops diabetes due to loss of beta-cells. (lu.se)
Tissue7
  • This means they can potentially produce new cells for any organ or tissue. (healthline.com)
  • In other words, dead people can yield living cells that can be converted into any cell or tissue in the body. (livescience.com)
  • Mature cells can be made or induced to become immature cells, known as pluripotent stem cells , which have the ability to become any tissue in the body and potentially can replace cells destroyed by disease or injury. (livescience.com)
  • Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)
  • Tissue regeneration and wound healing is promoted by the survival and regenerative capacities of tissue-resident or invading stem cells. (uni-muenchen.de)
  • Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
  • However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)
Harvard Stem Cell Institute1
  • We're thrilled to have Chad Cowan, an associate professor at Harvard University who is at the Harvard Stem Cell Institute. (medscape.com)
Mice2
  • As reported in a Penn news release , Xiaowei "George" Xu, MD, PhD, associate professor of Pathology and Laboratory Medicine and Dermatology at the Perelman School of Medicine, University of Pennsylvania, and colleagues published in Nature Communications a method for converting adult cells into epithelial stem cells (EpSCs), the first time anyone has achieved this in either humans or mice. (scienceblog.com)
  • The epithelial stem cells, when implanted into immunocompromised mice, regenerated the different cell types of human skin and hair follicles, and even produced structurally recognizable hair shaft, raising the possibility that they may eventually enable hair regeneration in people. (scienceblog.com)
Characteristics3
  • Thus, induced pluripotent stem cells represent a promising combination of adult and embryonic stem cell characteristics. (prnewswire.com)
  • While previous studies of cellular aggregates derived from human stem cells could not be considered accurate human embryo models because they lacked many of the defining characteristics of a post-implementation embryo, the Weizmann synthetic embryo models had all the structures characteristic of this stage, such as the placenta and yolk sac. (israel21c.org)
  • With a $50,000 grant from FRAXA Research Foundation, Dr. Clive Svendsen and his team at the University of Wisconsin grew neural stem cells that expressed the Fragile X mutation to help scientists better understand the gene characteristics. (fraxa.org)
Researchers9
  • A distinctive feature of this report is an end-user survey of 273 researchers (131 U.S. / 143 International) that identify as having induced pluripotent stem cells as a research focus. (prnewswire.com)
  • Researchers produced pluripotent stem cells from the fibroblast cells in the brain lining of human corpses. (livescience.com)
  • In the laboratory of Prof. Jacob Hanna at the Weizmann Institute of Science , researchers created complete models of human embryos from stem cells cultured in a lab grew them up to day 14. (israel21c.org)
  • The researchers started out with human pluripotent stem cells, which can differentiate into various cell types. (israel21c.org)
  • The researchers reprogrammed the pluripotent stem cells to an earlier (naïve) stage corresponding to day 7 of a natural human embryo, around the time it implants itself in the womb. (israel21c.org)
  • Prof. Jacob Hanna (center) and his team of researchers working on the development of the stem-cell embryo models. (israel21c.org)
  • Still, he notes that stem-cell researchers are developing more workarounds, including strategies using only chemical agents. (scienceblog.com)
  • The researchers analyzed more than 2,000 scientific papers and found adult stem cells are not replacing human embryonic stems cells in the laboratory. (sciencedaily.com)
  • The researchers examined stem cell research papers published between 1998 and 2010. (sciencedaily.com)
Biological3
  • Choose from Invitrogen gene editing and transfection technologies to build cell models that can better answer biological questions. (thermofisher.com)
  • Age, stage, and biological features encountered in tumor cells are important prognostic factors and are used for risk stratification and treatment assignment. (medscape.com)
  • These biological nanopores have been designed to protect the interior of the bacterial cell from leakage of toxic compounds while selectively allowing the entry of the molecules needed for cell growth and function. (ibecbarcelona.eu)
Adult stem2
Immature2
  • Загальні відомості про лейкоз Leukemia is a malignant condition involving the excess production of immature or abnormal leukocytes, which eventually suppresses the production of normal blood cells and results in symptoms. (msdmanuals.com)
  • [ 11 ] Immature dendritic cells respond to GM-CSF (not to macrophage colony-stimulating factor [M-CSF]) and become committed to generating dendritic cells, which are "professional" antigen-presenting cells (APCs). (medscape.com)
Cardiomyocytes1
  • Figure 2: Dr. Steinmetz's lab has engineered cardiomyocytes (heart muscle cells) from DCM patients that can be used to study the disease in a dish. (stanford.edu)
Types13
  • Since stem cells have the ability to turn into various other types of cells, scientists believe that they can be useful for treating and understanding diseases. (healthline.com)
  • There are several types of stem cells that can be used for different purposes. (healthline.com)
  • They make new red blood cells, white blood cells, and other types of blood cells. (healthline.com)
  • Adult stem cells can't differentiate into as many other types of cells as embryonic stem cells can. (healthline.com)
  • They can differentiate into all types of specialized cells in the body. (healthline.com)
  • In addition, their "unspecialized" nature allows them to differentiate into a wide variety of specialized cell types. (prnewswire.com)
  • This round shape is characteristic of mast cells and helps to differentiate them from other types of cells. (proprofs.com)
  • When a person loses hair, they lose both types of cells. (scienceblog.com)
  • Embryonic stem cells are considered the "gold standard" for all pluripotent stem cells, which are cells that can differentiate into all of the 220 cell types in the human body. (scienceblog.com)
  • Despite their unpredictability, Zhang notes that induced stem cells can still be used to make pure populations of specific types of cells, making them useful for some applications such as testing potential new drugs for efficacy and toxicity. (scienceblog.com)
  • Instead, the two cell types have proven to be complementary and any disruption of federal funding, they say, would negatively impact stem cell research overall. (sciencedaily.com)
  • The incentives to use both types of cell in comparative studies are high," says Jason Owen-Smith, a sociologist at University of Michigan. (sciencedaily.com)
  • Because use of the two cell types has become so intertwined, any federal policy that would deny funding for embryonic stem cell research "would derail work with a nascent and exciting technology," says Owen-Smith. (sciencedaily.com)
Embryo5
  • During the process of harvesting embryotic stem cells, the embryo is destroyed. (healthline.com)
  • The embryo models, created from adult human skin cells and cultivated stem cells, could improve fertility research. (israel21c.org)
  • Our stem cell-derived human embryo model offers an ethical and accessible way of peering into this box. (israel21c.org)
  • A stem cell-derived human embryo model equivalent to a day-14 embryo has all the compartments that define this stage: the yolk sac (yellow) and the part that will become the embryo itself, topped by the amnion (blue) - all enveloped by cells that will become the placenta (pink). (israel21c.org)
  • It must have the right cells in the right organization, and it must be able to progress - it's about being and becoming," said Hanna, whose lab created mouse embryo models last year. (israel21c.org)
Brain5
  • Now scientists have harvested such cells from the scalps and brain linings of human corpses and reprogrammed them into stem cells. (livescience.com)
  • We were able to culture living cells from deceased individuals on a larger scale than ever done before," researcher Thomas Hyde, a neuroscientist, neurologist and chief operating officer at the Lieber Institute for Brain Development in Baltimore, told LiveScience. (livescience.com)
  • Dr. Xinyu Zhao of the Waisman Center and Department of Neuroscience at University of Wisconsin-Madison joins us in this seminar to present Interrogate the Functions of FMRP in Brain Development Using Stem Cells. (fraxa.org)
  • Within the brain, the protein is involved in the development of specialized cells that process smell. (biolegend.com)
  • We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)
Cellular3
  • Traditionally, scientists have worked with both embryonic and adult stem cells for research tools, as well as for cellular therapy. (prnewswire.com)
  • In normal cells, glucose participates in cellular energy production through glycolysis as well as through its complete catabolism via the tricarboxylic acid (TCA) cycle and oxidative phosphorylation (OXPHOS). (hindawi.com)
  • We have taken a major interest in how mitochondria are genetically regulated, given their pivotal role in cellular energy metabolism and control of insulin secretion from beta-cells. (lu.se)
Therapy9
  • From feeder-based and feeder-free media to optimized passaging reagents and substrates to media for stem cell therapy research, Gibco products enable you to culture with confidence. (thermofisher.com)
  • A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
  • The Company has requested the Division's feedback to support the most expeditious pathway into the clinic to evaluate a novel cell therapy in patients suffering from COVID-19-related ARDS. (prnewswire.com)
  • Stem cell therapy in India is a safe, affordable, and legal treatment that relieves your ailments without any medicine. (medicaltourismco.com)
  • Dr. Anand Srivastava: What is Stem Cell Therapy? (medicaltourismco.com)
  • The Indian stem cell therapy ranges between $1,500 to $30,000 in India*, which further depends on the condition being treated. (medicaltourismco.com)
  • Therefore, the cost of stem cell therapy in India lets you save nearly 50% of the price back in the USA. (medicaltourismco.com)
  • All these diseases are readily treated by Stem Cell Therapy in India at a reasonable cost. (medicaltourismco.com)
  • Medical Tourism Corporation has partnered with the Global Institute Of Stem Cell Therapy and Research (GIOSTAR). (medicaltourismco.com)
Myeloid1
Research11
  • However, more research is needed to help understand the potential uses of amniotic fluid stem cells. (healthline.com)
  • Stem cell research and experimentation have been in process for well over five decades, as stem cells have the unique ability to divide and replicate repeatedly. (prnewswire.com)
  • Assoc. Prof. David Elliott leads the Heart Disease Group at the Murdoch Childrens Research Institute and he is a principal investigator of the Novo Nordisk Foundation for Stem Cell Medicine (reNEW). (edu.au)
  • In the late 1800s, Ehrlich conducted extensive research on cells involved in immune responses. (proprofs.com)
  • New research says studying both adult and embryonic stem cells can benefit medical science, but banning the study of either type could harm studies of the other. (sciencedaily.com)
  • This is an important study that systematically examines the co-authorship networks of stem cell research articles and uses those to understand the interactions between two complementary areas of research," says Julia Lane, program director for Science of Science & Innovation Policy at the National Science Foundation (NSF), which funded the study. (sciencedaily.com)
  • The research appears in the June 9 journal Cell . (sciencedaily.com)
  • As a result, induced pluripotent stem cells do not offer an easy solution to the difficult ethical questions surrounding embryonic stem cell research," he says. (sciencedaily.com)
  • With a $90,000 grant from FRAXA Research Foundation, Dr. Peng Jin's team from Emory University School of Medicine found that Fragile X causes an increase production of new cells, so they tested large numbers of drugs to find those that can correct this. (fraxa.org)
  • 2 iPS Cell-Based Drug Discovery, Drug Research Division, Sumitomo Dainippon Pharma. (nih.gov)
  • At International Society for Stem Cell Research-the big stem cell meeting-Yamanaka revealed one more factor. (medscape.com)
Scientists3
Human stem-cell1
  • Some of these were derived from reprogrammed adult skin cells and others were the progeny of lab-cultured human stem-cell lines. (israel21c.org)
Pathways2
  • Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)
  • Dendritic cells appear to develop in several pathways. (medscape.com)
Physiology1
  • check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)