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Embryonic Stem CellsInduced Pluripotent Stem CellsPluripotent Stem CellsStem CellsCell DifferentiationAdult Stem CellsHematopoietic Stem CellsNuclear ReprogrammingStem Cell TransplantationOctamer Transcription Factor-3Cell LineageCell Culture TechniquesNeural Stem CellsCells, CulturedSOXB1 Transcription FactorsEmbryoid BodiesEmbryo, MammalianMesenchymal Stromal CellsRegenerative MedicineGene Expression Regulation, DevelopmentalCell DedifferentiationCell LineCell ProliferationTeratomaMultipotent Stem CellsStem Cell NicheGerm LayersHematopoietic Stem Cell TransplantationStem Cell ResearchHomeodomain ProteinsTranscription FactorsSignal TransductionGene Expression ProfilingGene Expression RegulationFeeder CellsStage-Specific Embryonic AntigensMesenchymal Stem Cell TransplantationReverse Transcriptase Polymerase Chain ReactionEpigenesis, GeneticFibroblastsFlow CytometryEndodermRegenerationStem Cell FactorMice, Inbred C57BLGerm CellsCell SeparationKruppel-Like Transcription FactorsCell- and Tissue-Based TherapyNeuronsBone Marrow CellsBiological MarkersHematopoiesisPhenotypeTissue EngineeringCoculture TechniquesLeukemia Inhibitory FactorMyocytes, CardiacTotipotent Stem CellsLentivirusModels, BiologicalGene ExpressionEmbryo ResearchNeoplastic Stem CellsImmunohistochemistryRNA, MessengerTretinoinMice, SCIDBone Morphogenetic Protein 4Mice, TransgenicChimeraGreen Fluorescent ProteinsCell DivisionDNA-Binding ProteinsFetal Stem CellsCell SurvivalNeurogenesisActivinsMice, KnockoutAntigens, DifferentiationMesodermMicroRNAsGene TargetingCell ShapeBase SequenceTime FactorsKaryotypingOligonucleotide Array Sequence AnalysisDNA MethylationPlant StemsMolecular Sequence DataEmbryonic DevelopmentAntigens, CD34TransgenesGenetic VectorsColony-Forming Units AssayEmbryonal Carcinoma Stem CellsOsteogenesisPromoter Regions, GeneticAdipogenesis
FibroblastsAnalysis of human embryonic stemVitroPluripotencySomatic cellsDifferentiateIPSCsEmbryonal carcinoma cellsLineagesRegenerativeProtocolsNeurons from humanHepatocyte-like cellsTissuesProgenitor CellsNeuralCardiomyocytesEndothelialAdult stemHiPSCsBiologyMurineTransplantationHESCsESCsGrowth and differentiationSELF-RENEWALMechanismsCardiacMesenchymal stem2022GenesChondrogenic differentiationPopulationsSpecificationFetalMethylationDirect the differentiationEmbryosPancreaticCharacteristicsEndodermalOrganoidsLineageHepatocytesGermRegulatesFateGenerateInsulin-secreTissueCharacterizationDisease ModelingDevelopmentalPathwaysPrecursorEpithelial cellsOCT4DevelopmentFunctionalIntestinal2023VivoPromotesMolecular
Fibroblasts8
  • Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. (rndsystems.com)
  • Dr. Daniel Besser, Prof. Walter Birchmeier and Torben Redmer from the MDC, a member of the Helmholtz Association, used mouse embryonic fibroblasts (MEFs) in their stem cell experiments. (sciencedaily.com)
  • Pioneering work by the laboratory of Dr. Yamanaka showed that fibroblasts transduced with retroviral vectors expressing four transcription factors, Oct4, Klf4, Sox2 and cMyc can be reprogrammed to become pluripotent stem cells that appear almost indistinguishable from ESC. (bu.edu)
  • We have previously developed a single lentiviral vector expressing a stem cell cassett, named STEMCCA, capable of generating iPS cells from post-natal fibroblasts and peripheral blood with very high efficiency that became the industry standard. (bu.edu)
  • 6 Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic Stem Cells ( Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel ). (ecampus.com)
  • 7 Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts ( Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel ). (ecampus.com)
  • Researchers first multiplied skin cells (fibroblasts) from a 74 year-old donor to obtain the senescence characterized by the end of cellular proliferation. (eurekalert.org)
  • One way we did this was by converting adult fibroblasts, or skin cells, into interneurons. (lu.se)
Analysis of human embryonic stem3
  • Epigenetic Analysis of Human Embryonic Stem Cells: Basics, Computational Methods, Applications. (mpg.de)
  • Multivariate statistical analysis of epigenetic regulation with application to the analysis of human embryonic stem cells. (mpg.de)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Vitro22
  • These tissues provide a unique in vitro model to study cardiac physiology with a species-specific advantage over cultured animal cells in experimental studies. (wikipedia.org)
  • These lines will serve as tools to identify factor combinations to improve the in vitro differentiation of hESCs to functional blood stem cells. (ca.gov)
  • These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. (rndsystems.com)
  • This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. (jove.com)
  • Directing differentiation of human pluripotent stem cells into different endodermal lineages such that we may generate tissue for replacement therapies and discover novel in vitro methods to model and study human development and disease. (aiche.org)
  • Adult stem cell-based organoid technology is a versatile tool for the generation and long-term maintenance of near-native 3D epithelial tissues in vitro. (nature.com)
  • In vitro generation of human pluripotent stem cell derived lung organoids. (nature.com)
  • Sox2-low nPSCs do not show impaired neuroectoderm specification and differentiate efficiently in vitro into all embryonic germ lineages. (cam.ac.uk)
  • Strikingly, upon the removal of self-renewing cues Sox2-low nPSCs differentiate into both embryonic and extraembryonic cell fates in vitro and in vivo. (cam.ac.uk)
  • These old cells were reprogrammed in vitro to induced pluripotent stem cells (iPSC) and to rejuvenated and human embryonic stem cells (hESC): cells of all types can again be differentiated after this genuine "rejuvenation" therapy. (eurekalert.org)
  • They then completed the in vitro reprogramming of the cells. (eurekalert.org)
  • Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
  • Stage (S) 7 cells expressed key markers of mature pancreatic beta cells, including MAFA, and displayed glucose-stimulated insulin secretion similar to that of human islets during static incubations in vitro. (nih.gov)
  • The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
  • In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
  • Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
  • Although the genome-wide histone modification landscapes of mouse in vivo germ cells and in vitro PGCLCs have been profiled and several germline-specific properties of epigenetic reprogramming have been revealed, the study of genome-scale chromatin states in human FGCs is still challenging, due to the scarcity of materials and technical difficulties. (nature.com)
  • NPC markers are also useful in establishing and monitoring in vitro NPC culture differentiation. (sanbio.nl)
  • Owing to their manifold roles in health and disease, there have been intense efforts to synthetically generate blood vessels in vitro from human pluripotent stem cells (hPSCs). (bvsalud.org)
  • How can we specifically generate either arterial or venous endothelial cells (ECs) from hPSCs in vitro? (bvsalud.org)
  • In vitro experiments using NV virus-like particles (VLPs) directly showed NV VLP attachment to HBGA, resulting in VLP internalization into the cell ( 19 ). (cdc.gov)
  • From these critical studies, putative NV receptors were identified, and thus it was hypothesized that a successful in vitro cell culture system would most likely possess these receptors to support NV replication. (cdc.gov)
Pluripotency18
  • This pluripotency depends on a molecule -- E-cadherin -- hitherto primarily known for its role in mediating cell-cell adhesion. (sciencedaily.com)
  • If E-cadherin is absent, the stem cells lose their pluripotency. (sciencedaily.com)
  • In a first step they showed that the pluripotency of these stem cells is directly associated with the cell-adhesion molecule E-cadherin. (sciencedaily.com)
  • Thus, we have double proof that E-cadherin is directly associated with stem-cell pluripotency. (sciencedaily.com)
  • The Applied Biosystems TaqMan hPSC Scorecard Panel assesses pluripotency and trilineage differentiation potential using real-time qPCR assays and intuitive data analysis software. (thermofisher.com)
  • The TaqMan hPSC Scorecard Panels quantitatively confirms pluripotency (trilineage differentiation potential) for both embryonic and induced pluripotent stem cell lines. (thermofisher.com)
  • Self-renewal and pluripotency-the capacity to form any cell lineage-are inherent characteristics of induced pluripotent stem cells (iPSCs). (shinshu-u.ac.jp)
  • Induced pluripotency provides a tool to explore mechanisms underlying establishment, maintenance, and differentiation of naive pluripotent stem cells (nPSCs). (cam.ac.uk)
  • To account for this high level of functional variation, verifying the pluripotency of stem cells-from the generation of a new cell line through its expansion for an experiment-is crucial. (genengnews.com)
  • To address these difficulties, R&D Systems has developed new tools that aid in the verification of stem cell pluripotency during derivation, maintenance, and expansion. (genengnews.com)
  • Moreover, colony morphology is not truly indicative of pluripotency, since cells that are not fully reprogrammed can have similar morphology. (genengnews.com)
  • The GloLIVE™ Human Pluripotent Stem Cell Live Cell Imaging Kit and individual GloLIVE antibodies available from R&D Systems allow you to verify the pluripotency status of your stem cells by monitoring the expression of both positive and negative markers ( Figure 1 ). (genengnews.com)
  • Pluripotency of live human stem cell colonies was verified using a NorthernLights™ (NL) 493-conjugated Mouse Anti-Human SSEA-4 Monoclonal Antibody (green) and a NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (red). (genengnews.com)
  • Despite its broad use as the most common assay for assessing the pluripotency of stem cells, the teratoma assay has limitations. (genengnews.com)
  • Pluripotency means that the cells can differentiate into any cell type of the human body. (bayer.com)
  • Moreover, we find that the distal NDRs are enriched specifically for binding motifs of the pluripotency and germ cell master regulators such as NANOG, SOX17, AP2γ and OCT4 in human FGCs, indicating the existence of a delicate regulatory balance between pluripotency-related genes and germ cell-specific genes in human FGCs, and the functional significance of these genes for germ cell development in vivo . (nature.com)
  • Pluripotency factors including NANOG are continuously expressed during the transition from pluripotency to posterior epiblast to PGCs, thus bridging pluripotent and germline states. (bvsalud.org)
  • A process where fully differentiated or specialized cells revert to pluripotency or a less differentiated cell type. (bvsalud.org)
Somatic cells10
  • Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
  • The molecule also plays a crucial role in the reprogramming of somatic cells (body cells) into pluripotent stem cells. (sciencedaily.com)
  • In a second step the researchers investigated what happens when somatic cells that normally neither have E-cadherin nor are pluripotent are reprogrammed into a pluripotent stem cell state. (sciencedaily.com)
  • In this reprogramming technique, somatic cells are converted into induced pluripotent stem cells (iPSCs). (sciencedaily.com)
  • E-Cadherin is necessary for maintaining pluripotent stem cells and also for inducing the pluripotent state in the reprogramming of somatic cells," Dr. Besser said. (sciencedaily.com)
  • If E-cadherin is absent, somatic cells cannot be reprogrammed into viable pluripotent cells. (sciencedaily.com)
  • Understanding the molecular relationships is essential for using human somatic cells to develop stem cell therapy for diseases such as heart attack, Alzheimer's or Parkinson's disease or diabetes," Dr. Besser said. (sciencedaily.com)
  • NPCs can additionally be obtained by direct reprogramming of somatic cells from different cell lineage (PMID: 22445518). (sanbio.nl)
  • Thus, somatic cells (primitive streak) and PGCLCs are related-yet distinct-lineages segregated by temporally-dynamic signaling. (bvsalud.org)
  • The process that reverts CELL NUCLEI of fully differentiated somatic cells to a pluripotent or totipotent state. (bvsalud.org)
Differentiate14
  • Human engineered cardiac tissues (hECTs) are derived by experimental manipulation of pluripotent stem cells, such as human embryonic stem cells (hESCs) and, more recently, human induced pluripotent stem cells (hiPSCs) to differentiate into human cardiomyocytes. (wikipedia.org)
  • Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
  • In addition, their "unspecialized" nature allows them to differentiate into a wide variety of specialized cell types. (prnewswire.com)
  • While the appeal of embryonic cells has been their ability to differentiate into any type of cell, there has been significant ethical, moral, and spiritual controversy surrounding their use. (prnewswire.com)
  • Researchers have discovered what enables embryonic stem cells to differentiate into diverse cell types and thus to be pluripotent. (sciencedaily.com)
  • Researchers of the Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch have discovered what enables embryonic stem cells to differentiate into diverse cell types and thus to be pluripotent. (sciencedaily.com)
  • We had previously found that iSN04 promoted myogenic precursor cells (myoblasts) to differentiate into skeletal muscle cells and had hypothesized that the aptamer also enhanced differentiation of pluripotent stem cells. (shinshu-u.ac.jp)
  • This differs from previous studies which only identified conditions that allowed cells to differentiate to one fate or the other. (cam.ac.uk)
  • The defining characteristics of pluripotent stem cells, which include embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, are straightforward: their ability to self-renew and differentiate into ectoderm, mesoderm, and endoderm germ layers. (genengnews.com)
  • The most widely accepted method for determining whether a population of cells is truly pluripotent is the teratoma assay, that is, verifying in vivo a cell population's ability to differentiate into each of the three germ layers via teratoma formation in mice. (genengnews.com)
  • Neural progenitors are precursor cells able to further differentiate into various neuronal and glial cells. (sanbio.nl)
  • However, despite the common ability of hiPSCs and hESCs to differentiate into all 3 germ layers, their functional equivalence at the single cell level remains to be demonstrated. (jci.org)
  • The key to these cells' utility is their ability to differentiate into many different cell types depending on the stimulus received and they have been used in treatments for diseases such as cancer and neural degeneration, in rehabilitation of tetraplegic and paraplegic patients and even in dentistry 1 . (bvsalud.org)
  • Hematopoietic stem cells are cells isolated from blood and bone marrow that can differentiate into a variety of different specialized cells and suffer apoptosis 1 . (bvsalud.org)
IPSCs9
  • In addition to human iPSCs, it can be used to replate culture of primary neurons or to resuspend them for fact sorting or single cell sequencing. (jove.com)
  • The variation among induced pluripotent stem cells (iPSCs) in their differentiation capacity to specific lineages is frequently attributed to somatic memory. (nih.gov)
  • These data demonstrated that variations in the hematopoietic differentiation capacity of iPSCs are not attributable to somatic memories of their origins. (nih.gov)
  • Guiding iPSCs toward a target cell line has garnered much attention, but the process remains challenging. (shinshu-u.ac.jp)
  • Using various assays like RNA sequencing, cell staining and imaging, and molecular interaction and pathway analysis, the researchers investigated iSN04's effect on murine ESCs and iPSCs. (shinshu-u.ac.jp)
  • Signs of aging were erased and the iPSCs obtained can produce functional cells, of any type, with an increased proliferation capacity and longevity," explains Jean-Marc Lemaitre who directs the Inserm AVENIR team. (eurekalert.org)
  • But today, adult, mature cells - such as skin or blood cells - can be "reprogrammed" to generate induced pluripotent stem cells (iPSCs. (bayer.com)
  • Advanced culturing methods can make it possible to direct the differentiation of the iPSCs to a certain type of cell, like specific brain cells or heart cells. (bayer.com)
  • NPCs can be differentiated from embryonic stem cells (ESCs) but also from induced pluripotent stem cells (iPSCs) (PMID: 11731782). (sanbio.nl)
Embryonal carcinoma cells2
Lineages3
Regenerative14
  • At the same time, manipulating ESC differentiation toward a specific developmental pathway holds a great promise for their use in regenerative medicine. (bu.edu)
  • We now aim at using iPS cells for disease modeling disease modeling and study their potential for regenerative medicine. (bu.edu)
  • In addition to NSF, this research was funded by grants from the National Institutes of Health, and the Stanford Institute for Stem Cell Biology and Regenerative Medicine. (sciencedaily.com)
  • This has catalyzed research in both early embryogenesis as a model system for mammalian development as well as regenerative medicine as a renewable source of unspecialized cells which can be converted into nearly any cell type required by a patient. (utexas.edu)
  • By demonstrating the generation of cardiomyocytes from murine pluripotent stem cells, their concept shows promise as a regenerative therapy. (shinshu-u.ac.jp)
  • Unfortunately, regenerative medicine is not yet feasible outside a laboratory setting as available protocols to generate target cells are complicated and expensive. (shinshu-u.ac.jp)
  • Pluripotent stem cells have great potential for use in regenerative medicine and are an important tool for understanding developmental and disease mechanisms. (genengnews.com)
  • The results represent significant progress for research into iPSC cells and a further step forwards for regenerative medicine. (eurekalert.org)
  • Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
  • The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
  • BlueRock's cell therapy platform allows for the creation of a virtually unlimited number of specialized, differentiated cells that can be used to create regenerative medicines for many intractable diseases that come with significant cell loss or reduce the ability of cells to self-repair. (bayer.com)
  • Human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) are promising candidate cell sources for regenerative medicine. (jci.org)
  • Our results suggest that caution should be exercised before assuming that hiPSCs occupy a pluripotent state equivalent to that of hESCs, particularly when producing differentiated cells for regenerative medicine aims. (jci.org)
  • They may also serve as an unlimited source of therapeutically active cells for regenerative medi- cine. (lu.se)
Protocols11
  • Australia allowed the other download Human Embryonic and Induced Pluripotent Stem Cells: Lineage Specific Differentiation Protocols to impose software for methods in 1902 and Canada was also system centuries as emotional Goodreads by video until 1922. (idealnaja.pl)
  • Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
  • We have established in the lab robust differentiation protocols for the induction of intestinal specification of these disease-specific iPSC. (bu.edu)
  • However, technical challenges persist in the standardization of culture growth conditions and differentiation protocols. (the-scientist.com)
  • We show their efficacy to outline cell identity changes and unravel some of their underlying genetic mechanisms, whether during embryonic pancreas development or in experimental setups aiming at developing glucose-responsive insulin-secreting cells, such as pluripotent stem-cell differentiation or in adult islet cell reprogramming protocols. (unige.ch)
  • In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models. (wjgnet.com)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)
  • The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
Neurons from human1
  • Promotes differentiation of neurons from human and mouse embryonic stem (ES) cells (Crawford and Roelink, Elkabetz et al. (stemcell.com)
Hepatocyte-like cells3
  • Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
  • Direct and Indirect Contribution of Human Embryonic Stem Cell-Derived Hepatocyte-Like Cells to Liver Repair in Mice. (bioscience.co.uk)
  • Comparative Analysis of Human Embryonic Stem Cell and Induced Pluripotent Stem Cell-Derived Hepatocyte-Like Cells Reveals Current Drawbacks and Possible Strategies for Improved Differentiation. (mpg.de)
Tissues12
  • The possibilities arising from these characteristics have resulted in great commercial interest, with potential applications ranging from the use of stem cells in reversal and treatment of disease, to targeted cell therapy, tissue regeneration, pharmacological testing on cell-specific tissues, and more. (prnewswire.com)
  • Our studies also suggest that these hESC reporter lines will become valuable for investigators working on optimizing stem cell differentiation and culture for other tissues, as many of these reporter genes are not only restricted to HSC. (ca.gov)
  • In this study, we compared hematopoietic differentiation capacity of 35 human iPSC lines derived from four different tissues and four embryonic stem cell lines. (nih.gov)
  • Mouse embryonic stem (ES) cells are pluripotent cells, meaning that they can give rise to all tissues in the body. (utexas.edu)
  • Stem cells exist naturally in the body to replace damaged cells and tissues. (schoolscience.co.uk)
  • This research paves the way for the therapeutic use of iPS, insofar as an ideal source of adult cells is provided, which are tolerated by the immune system and can repair organs or tissues in elderly patients. (eurekalert.org)
  • Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
  • Alternatively, NPCs can be isolated from fresh nervous tissues using cell sorting with NPC markers (PMID: 11121071). (sanbio.nl)
  • Many studies have shown the utility of embryonic or adult stem cells for forming teeth and for regeneration of bone and soft tissues. (bvsalud.org)
  • Adult stem cells are classified as pluripotent and are undifferentiated cells that remain quiescent in tissues until stimulated, when they can create cell types that are compatible with the tissue in which they reside. (bvsalud.org)
  • Additionally, they can form specialized cell types from other tissues if they are transplanted 6 . (bvsalud.org)
  • Stromal cells are a mixed population that can create bone, cartilage and fat and also fibrous and connective tissues. (bvsalud.org)
Progenitor Cells4
  • Identification of multipotent luminal progenitor cells in human prostate organoid cultures. (nature.com)
  • Therefore, although S7 cells are not fully equivalent to mature beta cells, their capacity for glucose-responsive insulin secretion and rapid reversal of diabetes in vivo makes them a promising alternative to pancreatic progenitor cells or cadaveric islets for the treatment of diabetes. (nih.gov)
  • Figure 3) or PSA-NCAM are markers frequently used for intermediate progenitor cells and early immature neurons (PMID: 29625071). (sanbio.nl)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
Neural6
  • Reduces colony-forming efficiency of mouse neural stem cells (Androutsellis-Theotokis et al. (stemcell.com)
  • Exploring the genetic, molecular and biological features of neural differentiation process from embryonic and patient-specific stem cells may offer an unprecedented chance to understand normal and abnormal human brain development and diseases, with implications for drug development and potential cellular replacement therapy. (yale.edu)
  • What are neural stem cells (NSCs), neural progenitors, and neural precursors (NPCs)? (sanbio.nl)
  • Neural stem cells (NSCs) are undifferentiated neural cells with the capacity for long-term self-renewal and for differentiation into all types of neuronal and glial cells. (sanbio.nl)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
Cardiomyocytes3
  • Human pluripotent stem cells are differentiated into cardiomyocytes (hPSC-CMs) in culture through a milieu containing small-molecule mediators (e.g. cytokines, growth and transcription factors). (wikipedia.org)
  • Amend N, Thiermann H, Worek F, Wille T ( 2019 ) The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM). Toxicol Lett 308:1-6. (multichannelsystems.com)
  • However, when these pluripotent stem cells were treated after experiencing differentiating conditions for five days, specific marker genes were upregulated, and the cells committed to forming beating cardiomyocytes. (shinshu-u.ac.jp)
Endothelial3
Adult stem9
HiPSCs6
  • hESCs and hiPSCs are the primary cells used to generate hECTs. (wikipedia.org)
  • Kussauer S, David R, Lemcke H ( 2019 ) hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us. (multichannelsystems.com)
  • Here, we used single cell analysis to resolve the gene expression profiles of 362 hiPSCs and hESCs for an array of 42 genes that characterize the pluripotent and differentiated states. (jci.org)
  • Comparison between single hESCs and single hiPSCs revealed markedly more heterogeneity in gene expression levels in the hiPSCs, suggesting that hiPSCs occupy an alternate, less stable pluripotent state. (jci.org)
  • hiPSCs also displayed slower growth kinetics and impaired directed differentiation as compared with hESCs. (jci.org)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
Biology7
  • Our in vivo tracking tools will be broadly relevant and not restricted to studying the in vivo biology of blood forming cells. (ca.gov)
  • Cell Biology and Toxicology, 34(1), pp.51-64. (bioscience.co.uk)
  • These established tools enable new discoveries in fields such as immunology, inflammation and stem cell biology. (bdbiosciences.com)
  • While techniques for cell surface staining are relatively standard, optimal staining for intracellular markers often depends on the biology of the target protein. (bdbiosciences.com)
  • Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. (ecampus.com)
  • 1 Introduction to Pluripotent Stem Cells: Biology and Applications ( Maria Borowski and Gary S. Stein ). (ecampus.com)
  • Combining cell biology and electrophysiology, his work has the potential to create personalized disease models for future research. (lu.se)
Murine1
Transplantation3
  • Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
  • Using these reporter lines to monitor the induction of HSC "stemless' program, we are making progress in optimizing differentiation conditions toward generating functional HSCs for transplantation. (ca.gov)
  • Transplantation of pancreatic progenitors or insulin-secreting cells derived from human embryonic stem cells (hESCs) has been proposed as a therapy for diabetes. (nih.gov)
HESCs2
  • Pluripotent stem cells are those capable of differentiating into any type of tissue, hence the attractiveness of embryonic stem cells, or hESCs, also called ES cells, which are also pluripotent. (sciencedaily.com)
  • We describe a seven-stage protocol that efficiently converts hESCs into insulin-producing cells. (nih.gov)
ESCs4
  • The team, led by Associate Professor Tomohide Takaya from the Department of Agricultural and Life Sciences at Shinshu University, recently discovered that an anti-nucleolin aptamer, myogenetic oligodeoxynucleotide iSN04, induced myocardial differentiation in embryonic stem cells (ESCs). (shinshu-u.ac.jp)
  • Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
  • Moreover, the combination of gene modification and directed differentiation of ESCs provides perfect tool for disease modelling and drug discovery. (benthamscience.com)
  • What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)
Growth and differentiation2
  • This 3D scaffold, along with collagen - a major component of the cardiac extracellular matrix - provides the appropriate conditions to promote cardiomyocyte organization, growth and differentiation. (wikipedia.org)
  • All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
SELF-RENEWAL5
  • Despite these efforts, few genes are known to play a role in the processes of stem cell self-renewal and differentiation. (bu.edu)
  • We disproved past misconceptions in the field about the role of Yap1 concerning its function in ES cell self-renewal, showing that like the inner cell mass, Yap1 is dispensable for long-term maintenance in culture. (utexas.edu)
  • In particular, they recovered their capacity for self-renewal and their former differentiation potential, and do not preserve any traces of previous aging. (eurekalert.org)
  • A stem cell is an undifferentiated unit with powerful self-renewal properties that is capable of organizing other cell types in the body. (bvsalud.org)
  • Stem cells are defined as undifferentiated cells that have precursor properties, are capable of forming many different cell types and have the property of unlimited self-renewal 5 . (bvsalud.org)
Mechanisms7
  • This unique ability makes them ideal candidates to model early development allowing us to study the basic signaling mechanisms involved in stem cell fate determination. (bu.edu)
  • By studying the earlier events associated with intestinal differentiation comparing the normal and mutant cells we aim at discovering the basic mechanisms involved in tumor development in the gastrointestinal tract. (bu.edu)
  • Following his Ph.D., he performed a research fellowship in the lab of Jim Wells at Cincinnati Children's Hospital , where he turned his focus to understanding mechanisms that regulate embryonic development of endoderm-derived tissue (pancreas, liver, intestine) and utilizing human pluripotent stem cells (hPSCs) to understand human differentiation and development. (aiche.org)
  • Using model organisms to understand mechanisms by which the embryo develops, with a focus on specification, differentiation and organogenesis of tissue derived from the endoderm. (aiche.org)
  • However, the molecular mechanisms of how ES cells are differentiated remain much less understood. (utexas.edu)
  • To create next-generation cell therapies, we are also incorporating gene editing with an aim to increase potency and persistence, further reduce immunogenicity, or build safeguard mechanisms into the cells, making cell therapy an even more powerful tool to help patients suffering from intractable diseases. (bayer.com)
  • In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
Cardiac2
  • iSN04 treatment under differentiating conditions inhibited stem cell commitment to the cardiac lineage. (shinshu-u.ac.jp)
  • They can divide and form all types of differentiated adult cells in the body (neurones, cardiac cells, skin cells, liver cells, etc. (eurekalert.org)
Mesenchymal stem1
20221
Genes8
  • We aim at generating iPSC containing mutations in IRF5, Myd88, UNC93, TLRs and other relevant genes to test their role in mDC function compared to normal cells. (bu.edu)
  • The panel of genes was developed in collaboration with Alex Meissner, and based on his landmark publication ( Cell 144:439-452 (2011) ). (thermofisher.com)
  • This screen is capable of analyzing the expression of 48 genes simultaneously across dozens of different samples, and our gene list covers all three germ layers that arise during normal embryonic development, the trophectoderm, and epigenetic regulators of chromatin status. (utexas.edu)
  • Our signature-based screen established several categories of genes based on their comparative functions during the differentiation of ES cells. (utexas.edu)
  • Background: Bivalent chromatin domains consisting of the activating histone 3 lysine 4 trimethylation (H3K4me3) and repressive histone 3 lysine 27 trimethylation (H3K27me3) histone modifications are enriched at developmental genes that are repressed in embryonic stem cells but active during differentiation. (researchgate.net)
  • Here we show that bivalent domains and chromosome architecture for bivalent genes are dynamically regulated during the cell cycle in human pluripotent cells. (researchgate.net)
  • Central to this is the transient increase in H3K4-trimethylation at developmental genes during G1, thereby creating a 'window of opportunity' for cell-fate specification. (researchgate.net)
  • Numerous studies have shown that viral vectors can be used to implant osteoinductive growth factor genes directly into the paraspinal muscles or into cells that can subsequently be implanted next to the spine. (medscape.com)
Chondrogenic differentiation2
  • Promotes osteogenic, adipogenic, and chondrogenic differentiation of human mesenchymal cells (Jaiswal et al. (stemcell.com)
  • Promotes osteogenic, adipogenic, and chondrogenic differentiation of mouse mesenchymal cells (Tropel et al. (stemcell.com)
Populations5
  • While western blotting and other methods are useful for the examination of single proteins expressed by entire cell populations, flow cytometry allows the detection of multiple proteins simultaneously at the level of individual cells. (bdbiosciences.com)
  • However, given that bivalent domains can still be observed-albeit in lower proportion-in unipotent cells such as T cells and MEFs, an admixture of cell populations appears to be an unlikely explanation for the observed coexistence of these marks. (researchgate.net)
  • Moreover, single cell heterogeneity amongst stem cell populations may underlie important cell fate decisions. (jci.org)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
Specification3
  • The major shortcoming in generating functional hematopoietic stem cells (HSC) from pluripotent stem cells is the lack of adequate markers for specification and maintenance of functional human HSCs. (ca.gov)
  • State-of-the art technologies are used to investigate global molecular changes that arise during neuronal fate specification and differentiation from pluripotent embryonic stem (ES) cells. (yale.edu)
  • We use our simplified differentiation platform and single-cell RNA-sequencing to achieve further insights into PGCLC specification. (bvsalud.org)
Fetal4
  • However, the comprehensive chromatin state has not yet been analyzed for human fetal germ cells (FGCs). (nature.com)
  • Here we use nucleosome occupancy and methylation sequencing method to analyze both the genome-wide chromatin accessibility and DNA methylome at a series of crucial time points during fetal germ cell development in both human and mouse. (nature.com)
  • Finally, hPSC-derived PGCLCs can be easily purified by virtue of their CXCR4+PDGFRA-GARP- surface-marker profile and single-cell RNA-sequencing reveals that they harbor transcriptional similarities with fetal PGCs. (bvsalud.org)
  • VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)
Methylation3
  • Phosphorylation of EZH2 Activates STAT3 Signaling via STAT3 Methylation and Promotes Tumorigenicity of Glioblastoma Stem-Like Cells. (bioscience.co.uk)
  • https://pubmed.ncbi.nlm.nih.gov/23684459-phosphorylation-of-ezh2-activates-stat3-signaling-via-stat3-methylation-and-promotes-tumorigenicity-of-glioblastoma-stem-like-cells/ [Accessed 28 Feb. 2020]. (bioscience.co.uk)
  • Computational analysis of genome-wide DNA methylation during the differentiation of human embryonic stem cells along the endodermal lineage. (mpg.de)
Direct the differentiation1
  • We are combining novel engineered stromal cells with gene editing to explore a way to direct the differentiation of iPSC into hematopoietic progenitors and into a T cell/T reg lineage. (bu.edu)
Embryos2
Pancreatic4
Characteristics5
  • Thus, induced pluripotent stem cells represent a promising combination of adult and embryonic stem cell characteristics. (prnewswire.com)
  • Since 2007, a handful of research teams across the world have been capable of reprogramming human adult cells into induced pluripotent cells (iPSC), which have similar characteristics and potential to human embryonic stem cells (hESC). (eurekalert.org)
  • They have the physiological characteristics of "young" cells, both from the perspective of their proliferative capacity and their cellular metabolisms. (eurekalert.org)
  • Using this new "cocktail" of six factors, the senescent cells, programmed into functional iPSC cells, re-acquired the characteristics of embryonic pluripotent stem cells. (eurekalert.org)
  • To check the "rejuvenated" characteristics of these cells, the researchers tested the reverse process. (eurekalert.org)
Endodermal1
  • One focus of our lab is differentiating mouse ESC into intestinal epithelial cells in order to understand the complex signaling pathways involved in intestinal commitment from endodermal progenitors and undifferentiated stem cells. (bu.edu)
Organoids3
  • During this time, he pioneered efforts to generate 3-dimensional intestinal organoids from human pluripotent stem cells using an approach know as directed differentiation. (aiche.org)
  • We also provide an in-depth protocol for the generation of head and neck squamous cell carcinoma organoids and their subsequent use in semi-automated therapy screens. (nature.com)
  • Kretzschmar, K. & Clevers, H. Organoids: modeling development and the stem cell niche in a dish. (nature.com)
Lineage5
  • Similar uncertainties do Free Two-Day Shipping, Free responsible or academic download Human Embryonic and Induced Pluripotent Stem Cells: Lineage Specific to need robotics, Prime Video, Prime Music, and more. (idealnaja.pl)
  • Differentiation is a complex process involving actions of ES cell core factors, lineage specific regulators, epigenetic modifications, and chromatin remodelers. (utexas.edu)
  • an anti-nucleolin DNA aptamer, iSN04, can determine a cell's lineage during differentiation. (shinshu-u.ac.jp)
  • Ultimately, terminal cell differentiation commits to the cardiomyocyte lineage. (shinshu-u.ac.jp)
  • or movements in a free energy landscape such that lineage choices are paths between stable cell states. (lu.se)
Hepatocytes8
Germ4
  • Embryonic Stem Cells (ESC) are pluripotent undifferentiated cells capable of giving rise to cells from all three germ layers. (bu.edu)
  • Chromatin remodeling is important for the epigenetic reprogramming of human primordial germ cells. (nature.com)
  • Comparative molecular portraits of human unfertilized oocytes and primordial germ cells at 10 weeks of gestation. (mpg.de)
  • Generating primordial germ cell-like cells (PGCLCs) from human pluripotent stem cells (hPSCs) advances studies of human reproduction and development of infertility treatments, but often entails complex 3D aggregates. (bvsalud.org)
Regulates1
Fate2
  • This raises a pertinent question: Can regulating the fate of stem cells in clinical settings and at scale be made more economical? (shinshu-u.ac.jp)
  • Histone modifications and chromatin-associated protein complexes are crucially involved in the control of gene expression, supervising cell fate decisions and differentiation. (researchgate.net)
Generate5
  • Our goal is to generate a toolbox of human embryonic stem cell lines to monitor blood forming (hematopoietic) stem cell development in culture. (ca.gov)
  • To help overcome these bottlenecks, we will generate a suite of human embryonic stem cell reporter lines that can be used to monitor key milestones in blood stem cell development. (ca.gov)
  • To track the location and quantity of transplanted cells in recipient small animal model, we will generate hESC lines with in vivo reporter system that combines bioluminescent or PET imaging, and serum-based assay. (ca.gov)
  • We have also modified it to make it excisable and have used it to generate mouse and human iPS cells free of exogenous transgenes. (bu.edu)
  • Stem cell research endeavors to generate specific subtypes of classically defined "cell types. (bvsalud.org)
Insulin-secre1
  • Additional characterization using single-cell imaging and dynamic glucose stimulation assays revealed similarities but also notable differences between S7 insulin-secreting cells and primary human beta cells. (nih.gov)
Tissue5
  • The MDC researchers found that in contrast to the original cells, the new pluripotent cells derived from mouse connective tissue contained E-cadherin. (sciencedaily.com)
  • CML offers more-than-minimally manipulated cell and tissue procedures performed aseptically in our cleanroom in accordance with GMP / GTP quality compliance requirements . (cincinnatichildrens.org)
  • Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
  • A subset of NSCs is present in the developed nervous system and acts as a reservoir of cells for cell replacement and nervous tissue regeneration (PMID: 20110496). (sanbio.nl)
  • These cells are classified as totipotent and they can form any of the tissue types found in the adult body, in addition to having unlimited proliferation potential 6 . (bvsalud.org)
Characterization2
Disease Modeling1
Developmental2
  • Currently, we lack markers that we can use to pinpoint the developmental stage of human ES cell derived candidate hematopoietic stem cells (HSC), and whether they have established the correct molecular machinery required for proper function. (ca.gov)
  • In sum, this work defines wild-type levels of Sox2 as a restrictor of developmental potential and suggests perturbation of naive network as a mechanism to increase cell plasticity. (cam.ac.uk)
Pathways2
  • ES cells have been an invaluable resource for advancing fundamental understanding of global transcriptional and epigenetic regulations, signaling pathways, and noncoding RNA in mammalian systems. (utexas.edu)
  • While manipulating these two signaling pathways biases hPSC differentiation towards arterial and venous identities, efficiently generating these two subtypes of ECs has remained challenging until recently. (bvsalud.org)
Precursor2
  • May 20, 2020 In the bone marrow, blood stem cells via precursor cells give rise to a variety of blood cell types with various functions: white blood cells, red blood cells, or blood platelets. (sciencedaily.com)
  • We also propose that over time these DNA lesions would also accumulate in stem/precursor cells impairing their replicative capacity, thereby contributing to the complex aging phenotype. (yale.edu)
Epithelial cells1
  • FUT2 encodes an enzyme that produces histo-blood group antigens (HBGA) on the surface of epithelial cells and in mucosal secretions ( 21 , 22 ). (cdc.gov)
OCT41
  • In silico identification of a core regulatory network of OCT4 in human embryonic stem cells using an integrated approach. (mpg.de)
Development7
  • Human pluripotent stem cell-derived neurons are increasingly relevant in the areas of basic research, drug development, and degenerative medicine. (jove.com)
  • The strengths and focus of the Spence lab include using mouse models to study embryonic development of GI and associated organs, and in using 3-dimensional human models to study human development and disease. (aiche.org)
  • When it comes to the development of innovative cell therapy treatments, the development of pluripotent stem cells (PSCs) constitutes a quantum leap. (bayer.com)
  • Combinatorial binding in human and mouse embryonic stem cells identifies conserved enhancers active in early embryonic development. (mpg.de)
  • Here, we summarize how arterial or venous ECs arise during embryonic development. (bvsalud.org)
  • We explore the method on three circuits for haematopoiesis and embryonic stem cell development for commitment and reprogramming scenarios and illustrate how the method can be used to determine sequential steps for onsets of external factors, essential for efficient reprogramming. (lu.se)
  • The lack of an efficient cell culture system in which to study NoV infections has hindered development of antiviral drugs to control or limit NoV outbreaks ( 5 ). (cdc.gov)
Functional4
  • Aptamers are single-stranded pieces of DNA that bind to target proteins and are able to modulate signaling cascades during cell differentiation when a stem cell commits to a specific functional role or phenotype. (shinshu-u.ac.jp)
  • Generation of surrogate cells with stable functional identities is crucial for developing cell-based therapies. (unige.ch)
  • Intracellular flow cytometry is a powerful technique for the identification of cell types and the analysis of signaling and functional responses within cell lines and heterogeneous cell samples. (bdbiosciences.com)
  • Inserm's AVENIR "Genomic plasticity and aging" team, directed by Jean-Marc Lemaitre, Inserm researcher at the Functional Genomics Institute (Inserm/CNRS/Université de Montpellier 1 and 2), has recently succeeded in rejuvenating cells from elderly donors (aged over 100). (eurekalert.org)
Intestinal2
  • One major focus of the lab is the utilization of iPS Cells for the study of intestinal differentiation with a particular interest in Colorectal Carcinoma (CRC). (bu.edu)
  • A previous study, which used a 3-dimensional (3-D) intestinal model derived from INT-407 cells reported NoV replication and extensive cytopathic effects (CPE). (cdc.gov)
20231
  • Andreas Bruzelius defended his Ph.D. thesis 'Generating GABAergic interneurons through reprogramming and differentiation strategies' on Friday, 20 October 2023. (lu.se)
Vivo1
  • The Cell Manipulations Laboratory's experienced scientists can help you develop and scale up your ex-vivo gene transfer and cell therapy clinical trials or simply transfer your existing at-scale technology to our clean facilities which will enable you to move your research to a safe and effective patient treatment plan. (cincinnatichildrens.org)
Promotes3
  • Promotes differentiation of nociceptors from human pluripotent stem cells, in combination with several other small molecules (Chambers et al. (stemcell.com)
  • Promotes differentiation of retinal pigment epithelium from mouse ES cells (Osakada et al. (stemcell.com)
  • This kit, used in combination with the analysis of colony morphology, promotes the selection of high-quality, undifferentiated stem cell colonies, thereby reducing wasted time. (genengnews.com)
Molecular3
  • Therefore, after treatment, cells must be quickly fixed and subjected to stronger permeabilization conditions to allow the antibody to enter the nucleus and access the epitope within disrupted molecular complexes. (bdbiosciences.com)
  • At the single-cell level self-renewing Sox2-low nPSCs exhibit a naive molecular signature. (cam.ac.uk)
  • NPCs are distinct from differentiated cells of the nervous system at the molecular level. (sanbio.nl)