Pluripotent stem cell line cell lines genetically clone cells. Medical search. Frequent questions

Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from a somatic cell. (wikipedia.org)
citation needed] Since iPSCs can be derived directly from adult tissues, they not only bypass the need for embryos, but can be made in a patient-matched manner, which means that each individual could have their own pluripotent stem cell line. (wikipedia.org)
dubious - discuss] In his Nobel seminar, Yamanaka cited the earlier seminal work of Harold Weintraub on the role of myoblast determination protein 1 (MyoD) in reprogramming cell fate to a muscle lineage as an important precursor to the discovery of iPSCs. (wikipedia.org)
iPSCs are typically derived by introducing products of specific sets of pluripotency-associated genes, or "reprogramming factors", into a given cell type. (wikipedia.org)
Unlike the first generation of iPSCs, these second generation iPSCs produced viable chimeric mice and contributed to the mouse germline, thereby achieving the 'gold standard' for pluripotent stem cells. (wikipedia.org)
Derivation of autologous induced pluripotent stem cells (iPSCs) through direct reprogramming of easily accessible somatic cells holds the potential to transform the field of regenerative medicine. (biomedcentral.com)
Here, we summarize current reprogramming methodologies with a focus on the production of transgene-free or genetically unmanipulated iPSCs and highlight important technical details that ultimately may influence the biological properties of pluripotent stem cells. (biomedcentral.com)
In 2006, a major breakthrough was reported in Japan by Takahashi and Yamanaka, who described the generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts via over expression of defined transcription factors [ 2 ]. (biomedcentral.com)
Ideally, iPSC-based therapies in the future will rely on the isolation of skin fibroblasts or keratinocytes, their reprogramming into iPSCs, and the correction of the genetic defect followed by differentiation into the desired cell type and transplantation. (biomedcentral.com)
Several experimental strategies have been developed to derive iPSCs from differentiated somatic cells (summarized in Figure 1 ). (biomedcentral.com)
iPSCs have the great advantage that they can be generated from adults suffering from a known disease and then converted ("differentiated") into any cell type in the body. (ca.gov)
Our lab has been tasked with converting control and patient blood or skin cells, collected by scientists throughout the state of California, into high quality iPSCs. (ca.gov)
On the platform, we use the CRISPR-Cas system (CASS) to modify the genome of human induced pluripotent stem cell lines (IPSCs) by indels creation (nucleotide insertion/deletions), base editing or homologous directed recombination (HDR) approaches. (istem.eu)
isogenic IPSCs lines that are controls of patient IPSCs lines in which the genetic mutation has been corrected. (istem.eu)
In a recent study published in Stem Cell Reports, researchers evaluated the use of a dual clustered regularly interspaced short palindromic repeats (CRISPR)-Cas3 system for inducing multi-exon skipping (MES) among Duchenne muscular dystrophy (DMD)-patient-derived induced pluripotent stem cells (iPSCs). (medicineworldcouncil.com)
Dual crRNAs induced a large deletion at the dystrophin exon 45 to 55 area (340 kb) among HEK293T cells and in iPSCs, which could be applied to various DMD types. (medicineworldcouncil.com)
The inward-type dual CRISPR-Cas3 method effectively caused effective deletions of up to 344 kb among HEK293T cells and DMD patient-obtained iPSCs. (medicineworldcouncil.com)
Induced Pluripotent Stem Cells (iPSCs) are cells derived from non-pluripotent cells, such as adult somatic cells, that are genetically manipulated so as to return to an undifferentiated, pluripotent state. (asu.edu)
Research on iPSCs, initiated by Shinya Yamanaka in 2006 and extended by James Thompson in 2007, has so far revealed the same properties as embryonic stem cells (ESCs), making their discovery potentially very beneficial for scientists and ethicists alike. (asu.edu)
The recent development of induced pluripotent stem cells (iPSCs) and related technologies has caught the attention of scientists, activists, politicians, and ethicists alike. (asu.edu)
IPSCs gained immediate international attention for their apparent similarity to embryonic stem cells after their successful creation in 2006 by Shinya Yamanaka and in 2007 by James Thompson and others. (asu.edu)
Unlike embryonic stem cells, iPSCs are derived from adult human cells. (jax.org)
iPSCs "can be turned into disease-relevant cells such as nerve cells, allowing researchers to study disease biology in the very types of cells that become affected. (jax.org)
And with the development of CRISPR/Cas9 techniques, it is now possible to genetically manipulate iPSCs so that the effects of disease-related mutations can be studied. (jax.org)
However, the process of making iPSCs from patients with these diseases, and the subsequent genetic engineering of the cell lines, is "difficult, expensive and time consuming," says Ward. (jax.org)
It is well known that pluripotent stem cells, including embryonic stem cells (ESCs) and iPSCs can give rise to tumors and also result in immune rejection based on the research using immunodeficient mouse models. (atlasofscience.org)
To overcome this confusion, we designed a new mouse model by combining the theory of somatic animal cloning, tetraploid complementation, ESCs and iPSCs (Figure). (atlasofscience.org)
As a result, we can theoretically perform stem cell transplantation between iPSCs, and these mice could mimic the transplantation of iPSCs from the patients into themselves. (atlasofscience.org)
In addition, the iPSCs and their derivative tissue-specific stem cells should not form tumor. (atlasofscience.org)
Firstly, we can test immuno-rejection of iPSCs and various tissue specific stem cells, and to select suitable stem cell lines for transplantation. (atlasofscience.org)
Secondly, we can assess the tumor formation of iPSCs and other stem cell lines and select suitable stem cells for therapy. (atlasofscience.org)
We suggest the stem cell researchers all over the world to investigate the applicability of iPSCs and tissue specific stem cells for therapeutic applications and take advantage of this model. (atlasofscience.org)
CRISPR/Cas9-mediated TPM1 knockout in human induced pluripotent stem cells (iPSCs) enhanced hematopoietic progenitor development, increasing total megakaryocyte and erythroid cell yields. (biomedcentral.com)
Among PSCs, the donors available for induced pluripotent stem cells (iPSCs) are greatest, providing a potentially universal cell source for all types of cell therapies including cancer immunotherapies using natural killer (NK cells). (molcells.org)
Unlike primary NK cells, those prepared from iPSCs can be prepared with a homogeneous quality and are easily modified to exert a desired response to tumor cells. (molcells.org)
There already exist several protocols to genetically modify and differentiate iPSCs into NK cells, and each has its own advantages with regards to immunotherapies. (molcells.org)
In this short review, we detail the benefits of using iPSCs in NK cell immunotherapies and discuss the challenges that must be overcome before this approach becomes mainstream in the clinic. (molcells.org)
Among stem cells, induced pluripotent stem cells (iPSCs) have a number of features that make them ideal as the starting source. (molcells.org)
In this review, we examine the manufacturing and gene engineering of NK cells generated from iPSCs (iPS-NK) for ACT. (molcells.org)
To overcome this issue, we established immortalized megakaryocyte cell lines (imMKCLs) by introducing three transgenes, c-MYC, BMI1, and BCL-XL, sequentially into hematopoietic and megakaryocytic progenitor stage cells derived from iPSCs. (biomedcentral.com)
About ten years ago, biologists discovered that they could turn skin cells into "induced pluripotent stem cells" (iPSCs) by adding the right combination of growth factors. (gizmodo.com)

The use of embryonic stem cells has been a source of considerable controversy due to its sacrifice of human embryos in the blastocyst stage, which some people view as the destruction of human life . (citizendium.org)
Human embryos fertilized in the ordinary manner and harvested in the blastocyst stage have been used as an extensive source of stem cells for research purposes, and have been shown to possess therapeutic value in laboratory animals. (citizendium.org)
The most infamous study of embryonic stem cells asserted that cloned human embryos had been created via somatic cell nuclear transfer, and stem cells had been generated from these embryos. (citizendium.org)
Ethical objections to the use of human embryonic stem cells revolve around the destruction of human embryos in the blastocyst stage to obtain the stem cells. (citizendium.org)
Interest in using stem cells from cloned human embryos has revived after success by scientists in the United States and Korea. (bioedge.org)
Nature adds that rogue scientists might implant cloned embryos into wombs to create cloned children, a possibility which is widely condemned. (bioedge.org)
Similar to embryonic stem cells (ESCs), hpSCs are pluripotent cells that can be reproduced into any type of cell within the human body without using or destroying the viable human embryos. (pharmaceutical-technology.com)
The potential use of embryonic stem cells (ESCs) for cell replacement therapies is limited by ethical concerns and the technical hurdles associated with their isolation from human embryos. (biomedcentral.com)
Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
Under such circumstances, the idea of "therapeutic cloning" was proposed, indicating the generation of ESCs from SCNT embryos for therapeutic purpose. (benthamscience.com)
However, the derivation of human NT-ESCs goes with the destruction of clone embryos, leading to fierce ethical disputes. (benthamscience.com)
Advances in the biotechnology industry have increased scientists' understanding of the human genome and enhanced their ability to genetically modify eggs, sperm, and human embryos. (nyu.edu)
These scientists destroyed the embryos and derived stem cell lines. (nationalrighttolifenews.org)
Most embryos…formed one or two pronuclei at the time of removal from TSA, whereas a slightly higher portion of embryos cleaved…suggesting that some SCNT embryos did not exhibit visible pronuclei at the time of examination… Most cleaved embryos developed to the eight-cell stage…but few progressed to compact morula…and blastocyst. (nationalrighttolifenews.org)
Activation of embryonic genes and transcription from the transplanted somatic cell nucleus are required for development of SCNT embryos beyond the eight-cell stage…Therefore, these results are consistent with the premise that our modified SCNT protocol supports reprogramming of human somatic cells to the embryonic state. (nationalrighttolifenews.org)
A story in News.Com.Au-which runs stories from several Australian newspapers celebrates the cloning breakthrough because it means no embryos are used in the process! (nationalrighttolifenews.org)
But it is an important step in research because it doesn't require the use of embryos in creating the type of stem cell capable of transforming into any other type of cell in the body. (nationalrighttolifenews.org)
The Los Angeles Times has waded in to the junk biology game, assuring us that no embryos are threatened in human cloning-WHEN THE WHOLE POINT OF HUMAN CLONING IS TO CREATE AN EMBRYO! (nationalrighttolifenews.org)
Leeb, M. & Wutz, A. Derivation of haploid embryonic stem cells from mouse embryos. (nature.com)
Stem cell technologies have been dogged by controversy because of objections over the morality of sacrificing human embryos to produce the first human embryonic stem cell lines. (schlich.co.uk)
Importantly, therapeutic cloning research continued and ultimately contributed to the development of a new technology -induced pluripotent stem cell (iPS) technology-that holds out immense promise as a way of developing stem cell treatments that are 'customised' to an individual patient and can be created without the destruction of human embryos. (oxplore.org)
Scientists have applied somatic cell nuclear transfer to clone human and mammalian embryos as a means to produce stem cells for laboratory and medical use. (asu.edu)
Citizens disagree about whether we should destroy human embryos for their stem cells-and if so, which embryos, with whose money, under what regulatory guidelines. (eppc.org)
The holy grail of regenerative medicine-whatever one's ethical beliefs about destroying embryos-is to "reprogram" regular cells from one's own body so that individuals can be the source of their own rejection-proof therapies. (eppc.org)
That is to say, we risk turning developed cells into developing embryos, and thus risk engaging in the very activities of embryo destruction and human cloning that we seek to avoid. (eppc.org)
But we need to proceed carefully, recognizing that we are gaining new powers over human origins even when we do not use human embryos, and recognizing the danger of blurring the line between cellular parts and embryonic wholes. (eppc.org)
Far more controversial-and for good reason-are stem cells derived from destroyed human embryos. (eppc.org)
In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
They derived several human embryonic stem cell lines from these cloned embryos whose DNA was an exact match to the adult cell that donated the DNA. (news-medical.net)
Stem cells may be derived from adult tissues but the most potent are extracted from developing human embryos. (edu.au)

Stem cells are not specialized and the process of their specialization is called differentiation. (benthamscience.com)
Derivation and differentiation of haploid human embryonic stem cells. (nature.com)
Somatic cells are cells that have gone through the differentiation process and are not germ cells. (asu.edu)
However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
The term stem cell can be defined by two very important qualities: the cell has the ability to self-renew and, in a more general sense, the cell has not completed differentiation into its final state. (thefutureofthings.com)
This general definition includes a wide variety of cells with varying degrees of differentiation potential. (thefutureofthings.com)
Variation of DNA methylation on the IRX1/2 genes is responsible for the neural differentiation propensity in human induced pluripotent stem cells. (reprocell.com)
Pancreatic Differentiation of Stem Cells Reveals Pathogenesis of a Syndrome of Ketosis-Prone Diabetes. (reprocell.com)
Accordingly, scientists have developed protocols for the expansion, genetic modification and differentiation of stem cells to NK cells. (molcells.org)
The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)

Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
The first type of tissue engineering using stem cells was hematopoietic stem cell transplantation (HSCT), a surgical procedure in which hematopoietic stem cells (HSCs) are infused into a host to treat a variety of blood diseases, cancers, and immunodeficiencies. (asu.edu)
UCB, a source of hematopoietic stem cells (HSCs), has garnered attention from scientific and medical communities since its first successful use in a hematopoietic stem cell transplant (HSCT) in 1988. (asu.edu)
Identifying causal variants and genes from human genetic studies of hematopoietic traits is important to enumerate basic regulatory mechanisms underlying these traits, and could ultimately augment translational efforts to generate platelets and/or red blood cells in vitro. (biomedcentral.com)
To validate this putative candidate gene and to explore its function, we used CRISPR/Cas9 genome editing to discover that cultured TPM1 -deficient induced pluripotent stem cells enhanced hematopoietic progenitor cell formation. (biomedcentral.com)
Hematopoietic cells are almost totally absent. (medscape.com)
The microenvironment, consisting of lymphocytes, macrophages, fibroblasts, endothelial elements, and stroma in which hematopoietic cells reside, creates a regulatory niche that determines the local area network. (medscape.com)
Accelerated hematopoietic cell apoptosis has been demonstrated in virtually all IBMFS. (medscape.com)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

The iPSC technology was pioneered by Shinya Yamanaka and Kazutoshi Takahashi in Kyoto, Japan, who together showed in 2006 that the introduction of four specific genes (named Myc, Oct3/4, Sox2 and Klf4), collectively known as Yamanaka factors, encoding transcription factors could convert somatic cells into pluripotent stem cells. (wikipedia.org)
Patient-matched embryonic stem cell lines can now be derived using somatic cell nuclear transfer (SCNT). (wikipedia.org)
Totipotent cells have the capacity to differentiate to all cell types, including somatic cells, germ cells, and certain cells that exist outside the embryo and are important to fetal development that are termed extraembryonic cells. (citizendium.org)
To circumvent these obstacles, considerable effort has been invested in attempting to derive ESC-like cells by reprogramming somatic cells to an embryonic state. (biomedcentral.com)
Although exciting results have been achieved by means of somatic cell nuclear transfer, cell fusion, and culture-induced reprogramming [ 1 ], these procedures are technically demanding and inefficient and therefore unlikely to become a common approach for producing patient-specific pluripotent cells. (biomedcentral.com)
Somatic cell nuclear transfer (SCNT) is a technology applied in cloning, stem cell research and regenerative medicine. (asu.edu)
One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

Comparative analysis of human induced pluripotent stem cell-derived mesenchymal stem cells and umbilical cord mesenchymal stem cells. (reprocell.com)

While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
However, to realize the full potential of hiPSCs for disease modeling and drug screening, several challenges still need to be overcome, as there is the high variability between hiPSC lines, the risk of accumulating genetic aberrations when culturing hiPSCs, and the lack of standardized procedures for hiPSC generation as such (reviewed by Volpato and Webber, 2020 ). (frontiersin.org)
In addition, as the genetic identity of the donor egg from which the ESCs are derived most likely will differ from that of potential recipients, patients who receive ESC-derived cells or tissues may face the same complications that result from organ transplantation (for example, immunorejection, graft-versus-host disease, and need for immunosuppression). (biomedcentral.com)
Wutz, A. Haploid mouse embryonic stem cells: rapid genetic screening and germline transmission. (nature.com)
Genetic modification and screening in rat using haploid embryonic stem cells. (nature.com)
The two-colored single-strand annealing reporters facilitated the separation of cells with large genetic deletions. (medicineworldcouncil.com)
Research advocates attack President Bush for "banning stem cell research," while pro-life advocates lament a Republican administration and Congress that have banned nothing-not embryo destruction, not human cloning, not fetal farming, not genetic engineering. (eppc.org)
By isolating and reprogramming brain cells with dementia-causing genetic mutations, a team at JAX offers a powerful new research tool. (jax.org)
In vivo 2-color multiphoton imaging of genetic… Fibroblasts are cells that synthesize collagen and support the maintenance of connective tissue in the skin, tendons, and joints. (atlasofscience.org)
Through cell survival-based cDNA expression screens in neural progenitor cells, we identify a genetic variant of AGS Atp5g1 that confers cell resilience to metabolic stress. (elifesciences.org)
Examining the genetic code of these resilient cells revealed that Arctic ground squirrels may have a variant form of a protein called ATP5G1. (elifesciences.org)
TALEN and CRISPR/Cas9, when applied in GS cells, will be valuable tools in the study of spermatogenesis and for revealing the genetic mechanism of spermatogenic failure. (cyberleninka.org)
2004). In order to make GS cells more widely applicable for the study of spermatogenesis at the genetic and molecular levels, it is desirable to be able to modify their genome without disturbing their spermato-genic ability. (cyberleninka.org)

The Supreme Court's decision in Association for Molecular Pathology v. Myriad Genetics , Inc. [2] could be interpreted as paving the way for patenting genetically altered genome or gamete cells. (nyu.edu)
Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)
The best crRNA pairings for inducing MES in DMD-induced pluripotent stem cells were investigated, and a reporter method for enriching genome-edited cells was devised. (medicineworldcouncil.com)
To investigate the off-target mutagenesis possibility, whole-genome sequencing (WGS) of the genetically edited clones was performed, followed by an investigation of deletions linked to potential CRISPR RNA binding regions. (medicineworldcouncil.com)
Genome editing using the dual CRISPR-Cas3 system was performed in DMD patient-obtained induced pluripotent stem cell lines FF12020, CiRA00458, and CiRA00646. (medicineworldcouncil.com)
To identify putative causal genes from these data, we performed computational modeling using available genome-wide association datasets for platelet and red blood cell traits. (biomedcentral.com)
This topical collection on " Gene Editing " aims to provide a forum for discussions on the latest technical developments in the fields of general genome engineering technologies, including (i) the establishment of cell culture systems, and (ii) the development of established and emerging organismal models by CRISPR/Cas9 or similar genome engineering tools. (mdpi.com)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification, this process remains technically difficult. (cyberleninka.org)

They hypothesized that genes important to embryonic stem cell (ESC) function might be able to induce an embryonic state in adult cells. (wikipedia.org)
The term stem cell is also used in reference to any adult cells that are capable of assisting in the restoration of adult tissue via self-renewal. (citizendium.org)
There have been many false dawns in the field of embryonic stem cell research, but these results seem to confirm that it is possible to use adult cells to create genetically matched stem cell lines. (bioedge.org)
The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)

Unlike ESCs and iPSs, hpSCs can be produced in an identical form that allows each line to be immunologically compatible with millions of patients across the world. (pharmaceutical-technology.com)
Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)

Pluripotent stem cells hold promise in the field of regenerative medicine. (wikipedia.org)
Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) provide a valuable cell source for regenerative therapy, disease modeling, and drug screening. (biomedcentral.com)
The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)
The discovery of human pluripotent stem cells (PSCs) at the turn of the century opened the door to a new generation of regenerative medicine research. (molcells.org)

I-Stem's CRISPR platform is dedicated to the production of genetically modified human pluripotent stem cell lines for the study and treatment of single gene disorders. (istem.eu)
Pathological modeling of glycogen storage disease type III with CRISPR/Cas9 edited human pluripotent stem cells. (istem.eu)
Ward was on a research team that achieved the first successful merger of stem cell-derived cell types and CRISPR screening technologies. (jax.org)
George Church, the Harvard geneticist who pioneered CRISPR technology and is now spearheading an effort to clone the wooly mammoth back into existence, told Gizmodo he believes all of the project's cell biology goals are feasible. (gizmodo.com)
Ectopic expression in mouse cells and CRISPR/Cas9 base editing of endogenous AGS loci revealed causal roles of one AGS-specific amino acid substitution in mediating cytoprotection by AGS ATP5G1. (elifesciences.org)
In the present study, we tested TALEN and double-nicking CRISPR/Cas9 on GS cells, targeting Rosa26 and Stra8 loci as representative genes dispensable and indispensable in spermatogenesis, respectively. (cyberleninka.org)
Harvested GS cell colonies showed a high targeting efficiency with both TALEN and CRISPR/Cas9. (cyberleninka.org)

Pluripotent cells may differentiate to cells of most types, and multipotent cells are capable only of differentiating to certain types within a group of cells that perform similar functions. (citizendium.org)
reported a protocol to selectively differentiate hPSCs into CMs with nodal-type characteristics, which relies on co-culture with visceral endoderm-like cells and subsequent culture in a serum-enriched medium [ 12 ]. (biomedcentral.com)
Stem cells are undifferentiated cells that can shift, or differentiate, into specialized types of cells and serve as a repair system in the body by dividing indefinitely to replenish other cells. (asu.edu)
Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
At the top of the list comes the zygote-a fertilized egg, which of course has the ability to divide and differentiate into all cell types in the body and create a new organism. (thefutureofthings.com)
As the embryonic cells divide and the daughter cells differentiate, they become increasingly specific. (thefutureofthings.com)
The ICM continues to differentiate into three germ layers-ectoderm, mesoderm and endoderm, each of which follows a specific developmental destiny that takes them along an ever-specifying path at which end the daughter cells will make up the different organs of the human body. (thefutureofthings.com)
These adult stem cells are considered multipotent, having the ability to differentiate into different cell types, albeit with a more limited repertoire than embryonic stem cells. (thefutureofthings.com)
A particular field encouraged by the foundation is stem-cell research, with the great hope that it will result in the ability to get cells to differentiate into neurons and support cells to bridge the gap of a spinal cord injury. (thefutureofthings.com)

Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)
[5] Conditions such as anemias and immunodeficiencies, for which fetal tissue attempts largely failed, are now treated routinely with adult stem cells, including umbilical cord blood stem cells, [6] even while the patient is still in the womb. (lozierinstitute.org)

The facility is also near leading Californian research institutions that have already collaborated with ISCO on basic stem cell biology and therapeutic applications. (pharmaceutical-technology.com)
The junk biology is flying in the media's descriptions of the now accomplished human cloning. (nationalrighttolifenews.org)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)

To date, over 700 samples have been received from these Tissue Collectors and derivation of the first 50 patient-derived iPSC lines has been completed on schedule. (ca.gov)
Forward and reverse genetics through derivation of haploid mouse embryonic stem cells. (nature.com)

This produces either heterozygous human parthenogenetic stem cell lines that can be precisely HLA-matched or stem cell lines histocompatible with the oocyte donors or HLA homozygous hPSC that are histocompatible with the major segments of human population. (pharmaceutical-technology.com)
The HLA genotypes of stem cells obtained from such oocytes are heterozygous and are genetically cloned to the donors. (pharmaceutical-technology.com)
First year progress on grant ID1-06557, " Generation and Characterization of High-Quality, Footprint-Free Human Induced Pluripotent Stem Cell (iPSC) Lines From 3000 Donors to Investigate Multigenic Disease" has met all agreed-upon milestones. (ca.gov)
The goal of CIRM Grant ID1-06557 is to generate high quality induced pluripotent stem cells (iPSC) from blood and skin samples from 3000 donors, many of whom suffer from untreatable medical conditions, and place them in a Repository accessible to scientists around the world. (ca.gov)
Comparison of these "patient-derived" iPSC lines with those from healthy donors can help to illuminate the underlying cause of disease and also to serve as a system for discovering drugs to treat the disease in question. (ca.gov)
Many of these donors suffer from a variety of multigenic disorders and high-quality iPSC lines should prove invaluable in improving our understanding of these devastating diseases in addition to providing a starting point for the discover of effective therapeutics. (ca.gov)

The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)
The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)

The fibroblasts were engineered so that any cells reactivating the ESC-specific gene, Fbx15, could be isolated using antibiotic selection. (wikipedia.org)
Unlike induced pluripotent stem cells (iPSs), hPSCs do not manipulate the gene expression process back to a less differentiated stage, thereby avoiding the regulatory, ethical and safety obstacles. (pharmaceutical-technology.com)
Thanks to human induced pluripotent stem cell (iPSC) and gene-editing technologies, it's possible to derive every kind of brain cell type, insert dementia -related genes and study them in culture. (jax.org)
Eventually he cloned the GFP gene, but gave up on work to express the gene in mammalian cells. (pharmaceuticalintelligence.com)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

Cross-species transplantation was possible without the rejection of the human embryonic stem cells by the mice's immune systems because the mice were genetically modified to suppress certain immune responses that would have interfered with transplantation. (citizendium.org)
Studies have increasingly focused on the potential therapeutic effects of stem cell transplantation for neurological diseases [ 10 ]. (hindawi.com)
this limitation has been overcome via ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, to produce large quantities of these cells as an attractive source for cellular transplantation [ 16 - 18 ]. (hindawi.com)
Functional CMs derived from human pluripotent stem cells (hPSCs) would provide an unprecedented cell source for disease modeling, drug discovery, and cell transplantation therapy for CVD patients [ 3 ]. (biomedcentral.com)
This discovery not only laid the foundation for the research field of reprogramming, but also provided priceless sources for stem cell transplantation. (atlasofscience.org)
Compared with current mouse models for stem cell transplantation, our "Mouse Clone Model" has several advantages. (atlasofscience.org)
Furthermore, after the selection of applicable stem cells, we can investigate the appropriate stages of different stem cells for transplantation. (atlasofscience.org)
The Rosa26-targeted GS cells differentiated into fertility-competent sperm following transplantation. (cyberleninka.org)
On the other hand, Stra8-targeted GS cells showed defective spermatogenesis following transplantation, confirming its prime role in the initiation of meiosis. (cyberleninka.org)

But they showed, for the first time, that it is possible to create cloned embryonic stem cells that are genetically identical to the person from whom they are derived. (nationalrighttolifenews.org)
Reproductive Cloning - Use of a donor cell to create a new human genetically identical to the donor. (schlich.co.uk)
First of all, all the mice cloned from one inner cell mass would be genetically identical. (atlasofscience.org)
By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)

Once the SCNT is done, the cloning is over. (nationalrighttolifenews.org)
The cloning is completed when the SCNT is accomplished. (nationalrighttolifenews.org)
SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
This has led to a lot of interest in SCNT, which is best known as the method used to pioneer whole animal cloning technology, such as Dolly the sheep. (news-medical.net)
But SCNT can also be used to clone human cells for transplant or other therapies. (news-medical.net)
Another successful attempt at human SCNT was made using cells from two adult males. (news-medical.net)

Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
… "human clone" means an embryo that, as a result of the manipulation of human reproductive material or an in vitro embryo, contains a diploid set of chromosomes obtained from a single - living or deceased - human being, fetus, or embryo. (hinxtongroup.org)
2009). Physiologic Oxygen Concentration Enhances the Stem-Like Properties of CD133+ Human Glioblastoma Cells In Vitro. (igem.org)
Cost-effective blood cell generation will require novel strategies based on better knowledge of underlying mechanisms driving in vitro development. (biomedcentral.com)

Retrieved on December 04, 2023 from https://www.news-medical.net/life-sciences/Cloning-Human-Cells.aspx. (news-medical.net)

shows with high certainty that every NT-hESC line derived here originated from the respective patient donor and that these lines were not the result of enucleation failures and subsequent parthenogenetic activation. (citizendium.org)
Cellular Dynamics International (CDI) has continued to generate passage 5 iPSC lines from donor samples submitted to us by Tissue Collectors throughout the state of California. (ca.gov)
By the end of year three, we have generated iPSC clones from 1737 donor samples (out of 2670 received). (ca.gov)
However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
Therapeutic Cloning - Use of a donor cell to create pluripotent stem cells suitable for growing tissues for implantation into the donor or other patient. (schlich.co.uk)
Allogeneic-donor-derived cells can be genetically modified to eliminate expression of HLA-A. (ashpublications.org)
HLA-A disruption from donor cells is a step toward generating allogeneic cells as an off-the-shelf therapeutic. (ashpublications.org)
Long-term engraftment of allogeneic cells necessitates eluding immune-mediated rejection, which is currently achieved by matching for human leukocyte antigen (HLA) expression, immunosuppression, and/or delivery of donor-derived cells to sanctuary sites. (ashpublications.org)
Due to the lack of normal immune system of the recipient mice, it is uncertain whether tumor-formation and immune-rejection resulted from the mice themselves or the donor stem cells. (atlasofscience.org)
Since human induced pluripotent stem cell (iPSC)-platelet products can be supplied independently from the donor, it is expected to complement current platelet products. (biomedcentral.com)
Those were spindle removal, donor cell fusion, and cytoplast activation. (news-medical.net)
The mitochondrial DNA of the stem cells, however, matched the donor egg's mitochondrial DNA. (news-medical.net)
The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)
The resulting cells were pluripotent and could be differentiated into insulin-producing beta cells to restore the function of the pancreas in the donor. (news-medical.net)

These unlimited supplies of autologous cells could be used to generate transplants without the risk of immune rejection. (wikipedia.org)

If biotech scientists have the ability to manipulate the genes of an embryo or gamete cell for non-therapeutic purposes, it could be argued that these genetically modified cells are in fact patentable "inventions," given that the material was not, in that particular sequence, naturally occurring. (nyu.edu)

We now see a patent landscape where stem cell technologies and related therapies can, with very few exceptions, be protected via patents, provided the appropriate form of claim wording is used. (schlich.co.uk)
Stem cell technologies promise to be the next transformative medical technology offering therapies for conditions and diseases that are currently beyond medical science by creating replacement or supplementary tissues for a patient. (schlich.co.uk)
To date, it has been difficult to overcome primary brain cancer resulting from GBM, primarily because the cancer-initiating cells are suspected to be highly resistant to current cancer therapies. (igem.org)
These cells have been sought after as potential therapies for diseases ranging from heart disease to Parkinson's to cancer. (news-medical.net)

This long-sought technique may eventually let doctors create replacement cells for a wide variety of tissues from bits of a patient's own skin. (nationalrighttolifenews.org)
reagents made using a patient's own cells used to regenerate disease or damaged tissues 14,15 , once the stuff of science fiction, may become science fact. (schlich.co.uk)
Today, we can derive stem cells from a range of adult and newborn tissues: liver cells, kidney cells, brain cells, fat cells, and umbilical cord blood. (eppc.org)
They are found mainly in renewing tissues, such as the skin, the inner lining of the gastrointestinal tract and blood tissues. (thefutureofthings.com)
In addition to their ability to supply cells at the turnover rate of their respective tissues, they can be stimulated to repair injured tissue caused by liver damage, skin abrasions and blood loss. (thefutureofthings.com)
The ability of our body to regenerate some of its tissues is largely owed to the reserves of adult stem cells. (thefutureofthings.com)
Cloning of human cells is a technology that holds the potential to cure many diseases and provide a source of exactly matched transplant tissues and organs. (news-medical.net)

One group from CHA University in Seoul reported in Cell Stem Cell in April that it had managed to use cell nuclei from two men, aged 35 and 75, to create embryonic stem cell lines. (bioedge.org)
Dermal fibroblasts were taken from a 35-year old male and a 75-year-old male and used to create embryonic stem cells. (news-medical.net)

The stem cell facility will produce human parthenogenic stem cells (hpSCs) that are derived from parthenogenetically induced human oocytes. (pharmaceutical-technology.com)
The new International Stem Cell Corporation (ISCO) facility will produce human parthenogenic stem cells (hPSCs). (pharmaceutical-technology.com)

The cloning method is based on the fact that cytoplasmic factors in mature, metaphase II oocytes are able to reset the identity of a transplanted adult cell nucleus to an embryonic state. (news-medical.net)

In that debate, it was helpful to draw a clear distinction between reproductive cloning and therapeutic cloning. (oxplore.org)
Reproductive cloning is cloning to produce a live born baby. (oxplore.org)
Meanwhile, reproductive cloning has become virtually a non-issue, with few seriously suggesting that we should be striving to create clones of existing people. (oxplore.org)
In cloning, a distinction between reproductive applications and research enabled clearly beneficial research to proceed while controversial applications were set aside. (oxplore.org)
and the general public debate about reproductive cloning. (edu.au)

The latter has developed ten hPSC lines, including both HLA heterozygous and homozygous lines. (pharmaceutical-technology.com)

Upon introduction of reprogramming factors, cells begin to form colonies that resemble pluripotent stem cells, which can be isolated based on their morphology, conditions that select for their growth, or through expression of surface markers or reporter genes. (wikipedia.org)
A two-color single-strand annealing (SSA)-based reporter system was developed for Cas3 to enhance the genetically edited cells, followed by distance analysis. (medicineworldcouncil.com)
The team investigated whether the genetically edited population could be enhanced by sorting cells using the two-colored single-strand annealing reporter vectors among HEK293T cells. (medicineworldcouncil.com)

Our set-up enables automated, user-independent expansion of hiPSCs under fully defined conditions, and could be exploited to generate a large number of hiPSC lines for disease modeling, and drug screening at industrial scale, and quality. (frontiersin.org)
The Keller group described a transgene-independent method to generate sinoatrial node (SAN)-like pacemaker cells, which were identified as NKX2-5-negative CMs expressing SAN markers and displaying expected electrophysiological properties [ 13 ]. (biomedcentral.com)
Ph.D., professor and director of cellular engineering, has contracted with the National Institutes of Health to generate a collection of engineered iPSC brain cell lines for the Alzheimer's research community. (jax.org)
This method grants us the ability to generate a grand population of cloned mice, and each of them would be originated from the same and sole inner cell mass of the blastocyst. (atlasofscience.org)
Using Microfluidics to Generate Human Naïve and Primed Pluripotent Stem Cells. (reprocell.com)

Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells. (nature.com)

Cord blood banks are institutions designed to store umbilical cord blood (UCB) stem cells. (asu.edu)

The cultured SSCs, named germline stem (GS) cells, are now useful in research on various aspects of spermatogenesis. (cyberleninka.org)

This is by 4 early protein factors, the differentiated cells-such as fibroblasts, T and B cells-can return to pluripotent stem cells. (atlasofscience.org)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

The use of cell lines as "biological minipumps" to chronically deliver antinociceptive molecules into the pain-processing centers of the spinal cord represents a newly developed technique for the treatment of pain [ 3 ]. (hindawi.com)
In addition, the possibility of reprogramming adult stem cells back to a "pluripotent" (or embryonic-like) state raises the biological prospect of going back too far. (eppc.org)

This avoids the key immune rejection issues associated with other stem cell classes. (pharmaceutical-technology.com)
Because the mice used in this strategy had normal immune system (as contrast with immune-deficient mice), in theory, we can avoid tumor formation and immune-rejection during stem cell therapy. (atlasofscience.org)

The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
Till's work with stem cells in bone marrow, which produces the body's blood cells, helped form the field of modern hematology, a medical discipline that focuses on diseases related to the blood. (asu.edu)
Long before the controversy emerged over human embryonic stem cells, scientists and doctors began using first-generation stem cells from adult bone marrow. (eppc.org)
Adult stem cells can be used to accelerate bone or tendon healing , and they can induce cartilage progenitor cells to produce a better matrix and repair cartilage damage . (thefutureofthings.com)
Platelets are released from megakaryocytes (MKs) in the bone marrow and circulate as anucleate blood cells with a diameter of about 2-4 μm. (biomedcentral.com)
The etiology of bone marrow failure (BMF) includes defective stem/progenitor cells and/or stroma/accessory cells/growth factors, as well as deficient nonspecific nutrients or, as in the case of acquired aplastic anemia, immune-mediated abnormalities. (medscape.com)
Inherited bone marrow failure syndromes (IBMFS) are usually the result of intrinsic stem cell/progenitor defects. (medscape.com)
Pluripotent stem cells are biology's primordial matter: place them in the right environment, and they can transform into anything, from heart cells to bone marrow to gametes. (gizmodo.com)

However, supporters of embryonic stem cell research frequently contend that even the comparison to abortion is inappropriate, since while a several month old fetus might have sufficient neurological development to be conscious in some meaningful sense, a human embryo in the blastocyst stage has so little development that one can safely conclude that it cannot exist as a conscious being. (citizendium.org)
And this week, the New York Stem Cell Foundation Research Institute announced in Nature that it had created a line of cells from a woman with Type 1 diabetes. (bioedge.org)
Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
In particular, Cellular Dynamics International (CDI) has taken lease to approximately 5000 square feet of lab space at the Buck Institute for Research on Aging in Novato, CA. The majority of this space is located within the new CIRM-funded Stem Cell Research Building at the Buck Institute and was extensively reconfigured to meet the specific needs of this grant. (ca.gov)
The construction of this cell line is the first promising step in the regulation of GAL secretion from hTERT-immortalized BMSCs, and the potential application of this system may provide a stem cell-based research platform for pain. (hindawi.com)
US researchers have reported a breakthrough in stem cell research, describing how they have turned human skin cells into embryonic stem cells for the first time. (nationalrighttolifenews.org)
The method described on Wednesday by Oregon State University scientists in the journal Cell, would not likely be able to create human clones, said Shoukhrat Mitalipov, senior scientist at the Oregon National Primate Research Center. (nationalrighttolifenews.org)
Many people feared that allowing research on cloning techniques would lead to the creation of cloned babies. (oxplore.org)
2) It will allow the creation of new stem cell lines that can be used in medical research. (oxplore.org)
Scientists use immortal human cell lines in their research to investigate how cells function in humans. (asu.edu)
But they are also less equipped to produce every cell type of the body and less able to reproduce themselves indefinitely, which makes them less appealing to scientists interested in basic research. (eppc.org)
These moral perils are surely not a reason to oppose adult stem cell research, which deserves vigorous and expanded public support. (eppc.org)
Before leaving office, President Clinton sought to get around the existing law without actually changing it, by funding research on embryonic stem cells so long as the actual embryo destruction was paid for with private dollars. (eppc.org)
As opposed to many other known alternative treatments, it has not escaped our attention that tat-GFP labeled cells show normal morphology (Maddika 2005), a property especially appreciated and crucial for any conclusions to be made in the course of our research. (igem.org)
Stem cells are at the forefront of medical research and incite some of the most controversial ethical and religious debates worldwide. (thefutureofthings.com)
While regarded by many top scientists as the Holy Grail of medicine, others consider embryonic stem-cell research sacrilegious. (thefutureofthings.com)
Recent advances in the field of stem-cell research are giving hope to millions. (thefutureofthings.com)
Their work may be the development of research tools which allowed a great discovery leading to a line of transformative research, a moment of serendipity leading to discovery of a cure, or just contributions to the development of a new field or the mentoring of a new generation of scientists and clinicians. (pharmaceuticalintelligence.com)
Although the development of research tools, whether imaging tools, vectors, animal models, cell lines, and such are not heralded, they always assist in the pivotal discoveries of our time. (pharmaceuticalintelligence.com)
Dr. Tsien however realized the importance of Douglas's cloning work as pivotal for their research, contacted Douglas (who now due to the bad economy was working at a Toyota dealership in Alabama) and invited him to the Nobel Prize Award Ceremony in Sweden as his guest. (pharmaceuticalintelligence.com)
One such guest was one of my colleagues Dr. Leonard Cohen, who worked with Dr. Irwin Rose and Avram Hershko at the Institute for Cancer Research in Philadelphia a cell-free system from clams to discover the mechanism how cyclin B is degraded during the exit from the cell cycle ( from A. Hershko's Nobel speech ). (pharmaceuticalintelligence.com)
Viable cell lines from a dozen northern white rhinos are housed at the San Diego Zoo, the Leibniz Institute for Zoo and Wildlife Research in Berlin, and other tissue banks around the world. (gizmodo.com)
Embryonic stem cell technology is still at a preliminary research stage and announcements about its potential may be premature. (edu.au)
Experts from around the world are assessing the difficult issue of the extent to which embryonic stem cell research should be allowed to proceed, and to date there is little international consensus on this matter. (edu.au)
How, then, should embryonic stem cell research be regulated in Australia? (edu.au)
This issue was considered by the House of Representatives Standing Committee on Legal and Constitutional Affairs in its report entitled Human Cloning: Scientific, Ethical and Regulatory Aspects of Human Cloning and Stem Cell Research (hereafter the Andrews Report , after the Chair of the Committee, Mr Kevin Andrews, MP) released in September 2001. (edu.au)
In this article we examine embryonic stem cell research and explore the current regulatory framework associated with this research in Australia, with particular reference to the Andrews Report . (edu.au)

Scientists are unlikely to rush into creating human embryonic stem cell lines. (bioedge.org)
ISCO will simultaneously partner with leading scientists across the world to showcase the therapeutic applicability and the potential advantages of hPSC over other stem cells. (pharmaceutical-technology.com)
These lines are being used to produce large Distribution Banks that will be made available to scientists globally. (ca.gov)
Scientists have used cloning technology to transform human skin cells into embryonic stem cells, an experiment that may revive the controversy over human cloning. (nationalrighttolifenews.org)
In the second half of the twentieth century, scientists learned how to clone organisms in some species of mammals. (asu.edu)
In July 2005, for example, scientists announced that they had engineered adult mouse stem cells into usable mouse eggs, a technique that might one day allow for the creation of human eggs from ordinary human cells. (eppc.org)
A number of large biotech companies and scientists are looking toward stem cells as the basis for a therapeutic solution to cure such illnesses as blindness, diabetes and spinal cord injuries. (thefutureofthings.com)

The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)

For high-throughput cloning of edited cells, we use this automatic sampling machine for single cells and colonies. (istem.eu)

Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
Furthermore, the first source of stem cell technology is controversial. (schlich.co.uk)

Because they can propagate indefinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease. (wikipedia.org)
Furthermore, the use of diphtheria toxin -- which is far more toxic to human cells than mouse cells -- to destroy the human neurons in the mice reversed the observed improvements in motor function. (citizendium.org)
This result suggests that the observed increase in motor function was indeed produced by neurons derived from the human embryonic stem cells. (citizendium.org)

For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)

After sixteen weeks, the injured mice who received human stem cell injections experienced a significant improvement in the motor functions that had been impaired by their injuries. (citizendium.org)
Androgenetic haploid embryonic stem cells produce live transgenic mice. (nature.com)
found that when this variant form of ATP5G1 was introduced into the cells of mice, their mitochondria was better at coping with stress conditions, such as low oxygen, low temperature and poisoning. (elifesciences.org)

The researchers stopped well short of creating a human clone. (nationalrighttolifenews.org)

Anatomy53

Organisms15

Diseases9

Chemicals and Drugs58

Analytical, Diagnostic and Therapeutic Techniques and Equipment59

Phenomena and Processes58

Disciplines and Occupations7

Technology, Industry, Agriculture3

Information Science3

Health Care2

IPSCs41

Embryos30

Differentiation14

Hematopoietic9

Somatic9

Mesenchymal stem1

Genetic13

Genome9

Adult cells4

ESCs4

Regenerative6

CRISPR7

Differentiate10

Fetal2

Biology4

Progenitor cell1

Derivation2

Donors6

Clinical4

Gene5

Transplantation9

Identical4

SCNT6

Vitro4

20231

Donor14

Autologous1

Manipulate1

Therapies4

Tissues7

Create embryonic2

HPSCs2

Embryonic state1

Reproductive5

Heterozygous1

Reporter3

Generate5

Oocyte1

Umbilical cord1

Germline1

Fibroblasts2

Biological2

Immune rejection2

Bone8

Research27

Scientists7

Therapeutic applications1

Colonies1

Source of stem2

Neurons3

Neural progenitor cells1

Mice3

HUMAN CLONE1