• Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
  • Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
  • Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
  • Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
  • Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
  • Pluripotent stem cells can differentiate into any cell type found in an organism, making them a valuable tool for tissue engineering [ 1 - 3 ]. (biomedcentral.com)
  • Stiffness affects the type of cell resulting from differentiation: cells differentiate into neural, mesenchymal, and bone with increasing scaffold stiffness [ 19 ]. (biomedcentral.com)
  • However, scientists have found a way to reprogram these mature cells so that they "de-differentiate" and return to an unspecialized, proliferative state. (jove.com)
  • Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)
  • Support the growth and differentiation of human pluripotent stem cells by using Vitronectin XF™, a defined and xeno-free cell culture matrix. (stemcell.com)
  • Controlling the differentiation of human iPSCs into functional tissues remains difficult because complicated cell signaling networks regulate this process [ 5 - 9 ]. (biomedcentral.com)
  • Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
  • Master molds with inverted DLT were produced by 2-photon lithography (2-PL). To measure the mechanotransductive potential of DLT, mesenchymal stem cells were cultivated on silicone or collagen imprints with DLT. (lww.com)
  • Stem cell research is a broad field, dealing with embryonic and adult stem cells (e.g. mesenchymal and neural stem cells) or induced pluripotent cells. (eppendorf.com)
  • Novel Mesenchymal Stem Cell Spheroids with Enhanced Stem Cell Characteristics and Bone Regeneration Ability. (tohoku.ac.jp)
  • The dermal papilla consists of an egg-shaped accumulation of mesenchymal cells surrounded by ground substance that is rich in acid mucopolysaccharides (AMPs). (medscape.com)
  • Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
  • In particular, human cells seemed able to withstand the loss of three genes - CDK2, CCNE1, and CDK4 - that are essential to cell cycle function in chimps in pluripotent stem cells and in neural progenitor cells. (genomeweb.com)
  • We used ordinary differential equations to model the different cellular populations (stem, progenitor, differentiated) present in our scaffolds based on experimental results and published literature. (biomedcentral.com)
  • Our model considers these three cell states (stem, progenitor, and differentiated). (biomedcentral.com)
  • As they reported in Cell on Tuesday, the researchers relied on a series of genome-wide CRISPR-interference screens using growth analyses and high-throughput sequencing to search for loss-of-function changes affecting the proliferation of human or chimp pluripotent stem cells. (genomeweb.com)
  • Understanding the mechanisms regulating cell cycle, proliferation and potency of pluripotent stem cells guarantees their safe use in the clinic. (upf.edu)
  • Collectively these mechanisms lead to major differences in the overall effect of the signal on stem cell behavior in terms of proliferation versus differentiation. (biomedcentral.com)
  • In addition to stiffness, porosity affects stem cell differentiation and proliferation because contact induces these processes, but excessive contact inhibits aggregate growth. (biomedcentral.com)
  • STEM-CELLBANKER® maintains cell pluripotency, normal karyotype, and proliferation post thaw whilst also providing consistent high cell viability. (onenucleus.com)
  • AMSBIO has in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. (onenucleus.com)
  • Transcription Factor CTCFL Promotes Cell Proliferation, Migration, and Invasion in Gastric Cancer via Activating DPPA2. (nih.gov)
  • abstract = "Permanent lines of pluripotent stem cells can be obtained from humans and monkeys using different techniques and from different sources-inner cell mass of the blastocyst, primary germ cells, parthenogenetic oocytes, and mature spermatogonia-as well as by transgenic modification of various adult somatic cells. (elsevierpure.com)
  • The differentiation of patient-derived induced pluripotent stem cells (iPSCs) to committed fates such as neurons, muscle and liver is a powerful approach for understanding key parameters of human development and disease. (nih.gov)
  • Extended culture of DKC1-mutant iPSCs leads to progressive telomere shortening and eventual loss of self-renewal, indicating that a similar process occurs in tissue stem cells in dyskeratosis congenita patients. (nih.gov)
  • These findings in iPSCs from dyskeratosis congenita patients reveal that undifferentiated iPSCs accurately recapitulate features of a human stem cell disease and may serve as a cell-culture-based system for the development of targeted therapeutics. (nih.gov)
  • The lab employs a diverse range of model systems including patient-derived induced pluripotent stem cells (iPSCs), neuronal cultures and mouse models. (purdue.edu)
  • To create iPSCs, mature cells, like skin fibroblasts, are taken from a patient and put into culture. (jove.com)
  • iPSCs are potentially valuable in medicine, because a patient who needs a particular cell type-for instance, someone with a damaged retina due to macular degeneration-could receive a transplant of the required cells, generated from another cell type in their own body. (jove.com)
  • To create iPSCs, mature cells such as skin fibroblasts or blood cells from a person are grown in culture. (jove.com)
  • As with other types of stem cells, scientists are learning how to promote the differentiation of specific cell types from iPSCs efficiently, so that the needed cell types can be produced in adequate quantities. (jove.com)
  • A new STEM CELLS article from Lisa Porter (University of Windsor, Ontario, Canada) reveals an epigenetic mechanism involving the cell cycle regulator SPY1 used by normal fibroblasts to override reprogramming-induced senescence, thereby increasing the number of cells amenable to becoming induced pluripotent stem cells (iPSCs). (stemcellsportal.com)
  • At this virtual meeting - Dr. Kazuo Takayama from the renowned Center for iPS Cell Research and application (CiRA) at Kyoto University will be giving a unique insight into his recent pioneering research that has successfully modelled the SARS-CoV-2 life cycle in undifferentiated iPSCs, which were previously uninfectable. (onenucleus.com)
  • iMatrix™ Recombinant Laminin-511 is a high -performance cell culture matrix, compatible with a wide range of cell types that is particularly well suited to iPSCs. (onenucleus.com)
  • In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
  • The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
  • We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)
  • Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
  • Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
  • In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)
  • Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
  • Whilst LiCl exposure did not significantly alter the proportion of cells expressing markers for stem cells/undifferentiated cells (Oct4, SSEA4), neurons (Neurofilament M), astrocytes (GFAP) or cell cycle phase, the drug caused a 1.4-fold increase in total cell number. (plos.org)
  • Induced pluripotent stem (iPS) cells have shown potential for differentiation and may become a resource of functional neurons for the treatment of PD. (globalauthorid.com)
  • This study examined whether functional neurons could be efficiently generated from iPS cells using a five-step induction procedure combined with docosahexaenoic acid (DHA) treatment. (globalauthorid.com)
  • In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
  • Several academic and industry efforts are well under owned subsidiary Cyto Therapeutics, it had received way to produce dopaminergic neurons from stem approval by the Australian government to conduct a cells under conditions compliant with use in patients. (lu.se)
  • Without this, the patient cells lost in PD could be replaced by grafted community is left trying to interpret complex scien- immature human dopaminergic neurons [3, 5]. (lu.se)
  • Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. (lww.com)
  • 1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
  • However it remains unknown which endocytic pathways are active in brain endothelial cells. (helsinki.fi)
  • In present study we characterized human brain endothelial cells, hCMEC/D3, which are widely utilized as BBB in vitro model. (helsinki.fi)
  • As brain endothelial cells are polarized in vivo, the aim of the study was to demonstrate the cell polarization of hCMEC/D3 cells and to study the activity and functionality of different endocytic pathways as a function of cell polarization. (helsinki.fi)
  • The work presents a molecular map of blood vessels in the lymph nodes and defines the subpopulations of endothelial cells involved in leukocyte recruitment. (edu.pl)
  • The development of the inflammatory process is regulated by specialized endothelial cells that control the recruitment of leukocytes from the blood into the lymphoid tissues. (edu.pl)
  • In contrast, the diversity of blood endothelial cells (BECs) and their origin during inflammatory angiogenesis were not fully characterized so far. (edu.pl)
  • The study uses scRNA-seq to define and characterize different subsets of blood endothelial cells in the mouse lymph node. (edu.pl)
  • Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
  • And while in human pluripotent stem cells, miRNAs have been directly linked to the core pluripotency network, including the cell cycle regulation and the maintenance of the self-renewing capacity, their role in the onset of differentiation in other contexts, such as determination of neural cell fate, remains poorly described. (muni.cz)
  • Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
  • To get a new insight into pluripotency, we employed a plurifaceted experimental design combining expression proteomics with the proteome-wide integral solubility alteration (PISA) assay 7 to compare pluripotent cells with their isogenic progenies and parental cells as well as with allogeneic cells. (nature.com)
  • This is due to the lack of expression of cell cycle inhibitors, which ultimately determines naïve pluripotency by holding back differentiation. (upf.edu)
  • MicroRNAs (miRNAs) critically modulate stem cell properties like pluripotency, but the fundamental mechanism remains largely unknown. (biomedcentral.com)
  • The decreasing methylation repressed by miRNAs in turn activates the top miRNAs and pluripotent core factors, creating an active circuit system to modulate pluripotency. (biomedcentral.com)
  • While miRNAs directly repress pluripotent core factors to facilitate differentiation during the differentiation state, they also help stem cells to maintain pluripotency by activating pluripotent cores through directly repressing DNA methylation systems and primarily inhibiting development in the pluripotent state. (biomedcentral.com)
  • WDR5 interacts with the pluripotency factor Oct4/POU5F1 and is required for the efficient formation of induced pluripotent stem (iPS) cells. (thermofisher.com)
  • The team subsequently focused on the examination of effects of centrosome depletion on stem cell properties to find that the centrosome loss led to downregulation of regulators of pluripotency OCT-4 and NANOG and a concomitant increase in the expression of proteins which marked the initiation of differentiation program (namely p53, PAX-6, brachyury etc. (databasefootball.com)
  • hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)
  • b Cells were collected and heated in a narrow temperature range, with one sample incubated at 37 °C for expression measurements, protein aggregates were eliminated by ultracentrifugation and the soluble proteins were digested. (nature.com)
  • Since rate of autophagy in cells was not detected, it is impossible to determine, if there were changes in autophagy that were not reflected as changes in colocalization of these two proteins. (helsinki.fi)
  • To characterize hCMEC/D3 cells for the presence of specific endocytic pathways, proteins involved into each pathway were selected. (helsinki.fi)
  • To systematically reveal the roles of miRNAs in stem cells, this study first constructed a systems network [ 17 ] of interactions between miRNAs and proteins by combining multiple published datasets. (biomedcentral.com)
  • Then, genes for multiple transcription factors are delivered into the cells using a viral vector, and the transcription factor proteins are expressed using the cell's machinery. (jove.com)
  • For drug discovery research, AMSBIO offers assays, recombinant proteins and cell lines. (onenucleus.com)
  • To elaborate the role of this complex of eight proteins, we generated mice deficient in each of them, and found that this complex plays an important role in determining whether cells live or die. (oist.jp)
  • Centrosome duplicates once per cell cycle, with proteins PLK4 and STIL playing the pivotal role in the regulation of a key step in centrosome cycle - the duplication of centrioles. (databasefootball.com)
  • Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
  • Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
  • While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
  • Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
  • We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a robotic liquid handling unit that delivers footprint-free hiPSCs within 3 weeks with state-of-the-art efficiencies. (frontiersin.org)
  • The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
  • Our findings demonstrate that human stem cells have evolved a distinct reliance on several cellular pathways, including increased dependence on genes mediating lysosomal signaling and increased robustness to re-enter cell cycle," senior and co-corresponding author Alex Pollen, a regeneration medicine, stem cell, and neurology researcher with UCSF, said in an email. (genomeweb.com)
  • With a series of follow-up analyses, including additional gene knockdown, copy number variant, and RNA sequencing analyses on pluripotent stem cells from orangutans and from several human and chimpanzee representatives, the team found that genetic differences tended to fall on pathways involved in lysosomal signaling and cell-cycle progression. (genomeweb.com)
  • We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
  • Surprisingly, during the pluripotent state, the top important miRNAs do not directly regulate the pluripotent core factors as previously thought, but they only directly target the pluripotent signal pathways and directly repress developmental processes. (biomedcentral.com)
  • Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)
  • American Journal of Respiratory Cell and Molecular Biology. (lu.se)
  • Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)
  • While previously differentiation was thought to be irreversible, mature cells can revert from the terminally differentiated state into the stem state in both nature and the lab [ 9 , 15 ]. (biomedcentral.com)
  • Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
  • Transplantation of pluripotent stem cell-derived cardiomyocytes represents one regenerative therapeutic concept that aims at adding new myocytes to the injured heart. (cnic.es)
  • We have previously demonstrated that hair follicles contain nestin-expressing pluripotent stem cells that can effect nerve and spinal cord repair upon transplantation. (googleapis.com)
  • Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
  • Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
  • Based on 16 CRISPR interference screens and essential and nonessential gene Dependency Map analyses, they went on to uncover 75 genes with species-specific growth effects in stem cells. (genomeweb.com)
  • Despite different origin, all pluripotent lines demonstrate considerable similarity of the major biological properties: active self-renewal and differentiation into various somatic and germ cells in vitro and in vivo, similar gene expression profiles, and similar cell cycle structure. (elsevierpure.com)
  • However, these recent studies have mostly focused on individual gene functions in stem cells although genome-wide data might be employed, and the conclusions drawn from these current studies are unavoidably biased on genes selected by these studies. (biomedcentral.com)
  • This complexity of miRNA interaction network presents a challenge for conventional approaches like gene-knockout to unbiasedly capture the real mechanisms of miRNA functions in stem cells. (biomedcentral.com)
  • WD repeat domain 5 (WDR5) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. (thermofisher.com)
  • His lab utilizes a combined approach of molecular genetics, pharmacology, biochemistry, cell and developmental biology to understand the role of gene-environment interactions between metal exposure and neurodevelopmental and neurodegenerative diseases including Parkinson's Disease, Alzheimer's Disease and Huntington's Disease. (purdue.edu)
  • This study explores the use of a simple human neurosphere-based in vitro model to characterise the pharmacological and toxicological effects of LiCl and VPA using gene expression changes linked to phenotypic alterations in cells. (plos.org)
  • In addition, in order to phenotypically anchor the gene expression data, changes in the heterogeneity of cell subtype populations and cell cycle phase were monitored using flow cytometry. (plos.org)
  • We demonstrated that DHA, a ligand for the RXR/Nurr1 heterodimer, significantly activated expression of the Nurr1 gene and the Nurr1-related pathway in iPS cells. (globalauthorid.com)
  • For example, we have shown that the CCR4-NOT complex is essential for insulin production and immune cell differentiation, and that it is important for gene regulation during early development. (oist.jp)
  • Now the cell "believes" that it can grow, but in reality, without the gene transcription factors it wouldn't be able to grow. (medium.com)
  • CaMKII inhibition in human primary and pluripotent stem cell-derived chondrocytes modulates effects of TGFbeta and BMP through SMAD signaling. (ca.gov)
  • Phosphatidylinositol 3 kinase (PI3K) modulates manganese homeostasis and manganese-induced cell signaling in a murine striatal cell line. (purdue.edu)
  • The involvement of microRNAs (miRNAs) in orchestrating self-renewal and differentiation of stem cells has been revealed in a number of recent studies. (muni.cz)
  • The comprehensive miRNA profiling presented here reveals novel sets of miRNAs differentially expressed during human neural cell fate determination in vitro. (muni.cz)
  • Our data also indicates that miRNA clusters enriched in NSCs share the target-determining seed sequence with cell cycle regulatory miRNAs expressed in pluripotent hESCs. (muni.cz)
  • This study systematically analyzes multiple-omics data and builds a systems physical network including genome-wide interactions between miRNAs and their targets to reveal the systems mechanism of miRNA functions in mouse pluripotent stem cells. (biomedcentral.com)
  • Globally, miRNAs directly repress the pluripotent core factors during differentiation state. (biomedcentral.com)
  • Furthermore, at the pluripotent state miRNAs predominately repress DNA methyltransferases, the core enzymes for DNA methylation. (biomedcentral.com)
  • MiRNAs vary their functions with stem cell states. (biomedcentral.com)
  • On the other hand, miRNAs like let-7 induce stem cell differentiation [ 11 ]. (biomedcentral.com)
  • Therefore, these studies only provide partial mechanisms of miRNA functions in stem cells, and the overall systems mechanisms of how miRNAs regulate stem cell processes remain largely elusive. (biomedcentral.com)
  • This present study employed systems physical network approaches [ 17 ] and constructed a comprehensive and unbiased map of genome-wide interactions between miRNAs and their targets to investigate the global basis of miRNA roles in pluripotent stem cells, from the pluripotent self-renewal state to the differentiation state. (biomedcentral.com)
  • Results of the present study lay a conceptual framework for future studies and applications of miRNAs in stem cells. (biomedcentral.com)
  • A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy. (genomeweb.com)
  • [ 1 ] Most of the neutrophils are contained in the bone marrow, either as mitotically active (one third) or postmitotic mature cells (two thirds). (medscape.com)
  • Загальні відомості про мієлопроліферативні захворювання Myeloproliferative neoplasms are clonal proliferations of bone marrow stem cells, which can manifest as an increased number of platelets, red blood cells (RBCs), or white blood cells (WBCs). (msdmanuals.com)
  • Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
  • Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)
  • Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)
  • Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
  • Use Vitronectin XF™ with mTeSR™1 , mTeSR™ Plus , TeSR™-E8™ , or TeSR™-AOF medium to provide a defined culture system for the maintenance of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells under feeder-free conditions. (stemcell.com)
  • Stemfit® is the leading feeder-free medium for ES/iPS cell culture that combines superior expansion rates with lower media usage. (onenucleus.com)
  • Short telomeres, a hallmark of dyskeratosis congenita, impair tissue stem cell function in mouse models, indicating that a tissue stem cell defect may underlie the pathophysiology of dyskeratosis congenita. (nih.gov)
  • Combining tissue engineering and stem cell biology allows us to generate three-dimensional human tissue constructs (engineered heart tissue) with organotypic function that structurally resemble native myocardium. (cnic.es)
  • Tissue engineering combines biomaterials and cells, creating functional structures that can replace damaged regions of tissue [ 1 , 2 ]. (biomedcentral.com)
  • For example, retinal cells that could be used to replace damaged retinal tissue. (jove.com)
  • Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
  • of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)
  • This review summarizes the research data on the genetic and epigenetic stability of different lines of pluripotent stem cells after long-term in vitro culture. (elsevierpure.com)
  • Gordeeva, OF & Mitalipov, SM 2008, ' Pluripotent stem cells: Maintenance of genetic and epigenetic stability and prospects of cell technologies ', Russian Journal of Developmental Biology , vol. 39, no. 6, pp. 325-336. (elsevierpure.com)
  • This innovation showcase presentation at ISCCR 2021 is sponsored by AMSBIO in association with the manufacturers Stemfit® cell culture media, iMatrix™ recombinant laminin extracellular matrix and STEMCELLBANKER® cryopreservation solutions. (onenucleus.com)
  • NEW YORK - A team led by investigators at the University of California, San Francisco has combined loss-of-function screening and comparative genomics to tease out genetic dependencies that distinguish human stem cells from those in nonhuman primates. (genomeweb.com)
  • The findings build on prior research looking at rare, large-effect mutations that distinguish humans and nonhuman primates, Pollen explained, noting that the current analyses focused on more subtle, "long tail" mutations with species-specific effects in the pluripotent stem cell type considered. (genomeweb.com)
  • Scientists hope to gain valuable insights from the study of stem cells, e.g. in developmental biology. (eppendorf.com)
  • This course is a co-arrangement between the Research School in Stem Cell Biology, the Lund University Neuroscience Graduate School, the StemTherapy FACS Core Facility at Lund Stem Cell Center, and the MultiPark Cellomics and Flow Cytometry Core Facility. (lu.se)
  • Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)
  • Fig. 2: Analysis of cell-essential genes. (nature.com)
  • Tcf1, but not Tcf3, is recruited to a palindromic motif enriched in the promoter of cell cycle repressor genes, such as p15Ink4b, p16Ink4a and p19Arf, which mediate the Wnt-dependent anti-proliferative effect in mESCs. (upf.edu)
  • Then, typically, genes for transcription factors, are delivered by viral vectors into the cell nuclei, where they are incorporated into the genome. (jove.com)
  • Furthermore, DHA dramatically upregulated the endogenous expression levels of neuroprotective genes ( Bcl-2, Bcl-xl, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor) and protected against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced apoptosis in iPS-derived neuronal precursor cells. (globalauthorid.com)
  • Furthermore, we found that DHA treatment in iPS cell-grafted rats significantly downregulated the mRNA expression of embryonic stem cell-specific genes (Oct-4 and c-Myc) in the graft and effectively blocked teratoma formation. (globalauthorid.com)
  • In this process, we use four main growth genes(Oct4, Sox2, Klf4, and c-Myc) to reprogram and "trick" the cell into becoming pluripotent. (medium.com)
  • Moreover, the team noted that the cell-cycle pathway changes unearthed in the CRISPR-based stem cell screen involved genetic shifts that appeared to be evolutionarily derived in humans relative to the ancestral forms found in chimp and orangutan stem cells. (genomeweb.com)
  • As induced pluripotent stem (iPS) cells maintain the genetic information of the donor, they can be used to model individual differences in SARS-CoV-2 infection in vitro. (onenucleus.com)
  • Our research is defining the normal mechanisms of DNA replication and repair, with the aim of understanding how it is deregulated in cancer cells. (birmingham.ac.uk)
  • The molecular mechanisms by which such stem cells preserve the ability to switch to a pluripotent state are being investigated. (ny.gov)
  • We start off with two types of cells, an oocyte (from embryos) and a somatic cell (any cell from the body) and exclude the nucleus from the oocyte and isolate the nucleus from the somatic cell. (medium.com)
  • Everything in the oocyte, excluding the nucleus, has the environment for cell growth since its origins are from an embryo and when we transfer the somatic cell nucleus it reprograms the cell to become pluripotent. (medium.com)
  • Given that p53 has been shown to mediate consequences of centrosome loss in mice and human somatic cell lines, the team examined its role in the context of centrosome loss in human stem cells. (databasefootball.com)
  • In 2006, Takahashi and Yamanaka discovered that mature cells could be reprogrammed back into a pluripotent state, which introduced the option of using patient derived stem cells as a tool for engineering tissues [ 4 ]. (biomedcentral.com)
  • We drive the models in optimization cycle towards better structures as assessed by the used metrics on DOPE score, retrospective distance restraint realization and others. (helsinki.fi)
  • In this line of research, we are particularly interested in studying the interplay between cardiac work and cell cycle activity. (cnic.es)
  • Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
  • Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
  • This expertise in cell culture and the ECM allows AMSBIO to partner with clients in tailoring cell systems to enhance organoid and spheroid screening outcomes using a variety of 3D culture systems, including organ-on-a-chip microfluidics. (onenucleus.com)
  • The symposium will cover a range of topics, including advances in single-cell analysis, genome editing, high-throughput screening, and imaging technologies. (oist.jp)
  • Cell-based double-screening method to identify a reliable candidate for osteogenesis-targeting compounds. (tohoku.ac.jp)
  • Pyruvate enters the tricarboxylic acid cycle to generate energy. (spandidos-publications.com)
  • The proposed candidate is a universal cell therapy designed to generate new articular cartilage in these defects and interrupt the cycle of degeneration. (ca.gov)
  • The DNA within the nuclei of our cells carries the information to generate the machinery of the cell, the cell itself, our tissues and then the whole human. (birmingham.ac.uk)
  • However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. (lww.com)
  • The course and physiological effects of the interaction of leukocytes with vascular wall cells have been studied for years and relatively well understood. (edu.pl)
  • Butcher: A molecular map of murine lymph node blood vascular endothelium at single cell resolution Nat Commun 2020 Jul 30;11:3798. (edu.pl)
  • In order to evaluate if activation of this axis has a protective effect in ageing-induced kidney disease we generated a transgenic rat model (TGR(SM22hACE2)) overexpressing human ACE2 in vascular smooth muscle cells. (bvsalud.org)
  • The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)
  • This document does not apply to cell lines used for in vivo application in humans, clinical applications or therapeutic use. (iso.org)
  • 2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
  • Cell Stem Cell 19 , 569-572 (2016). (nature.com)
  • Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
  • A second press release [2] has indicated stem cell source, resulting in widespread excitement that the program is planning to move forward very rapidly, with all of the patients being enrolled in the Correspondence to: Roger A. Barker, John van Geest Centre first quarter of 2016 and interim results being shared for Brain Repair, Department of Clinical Neurosciences, Univer- in October 2016. (lu.se)
  • Human embryonic kidney cells 293 (HEK-293) and hPREP knockout cell line created from them by using CRISPR/Cas9-silencing were used in the experiments. (helsinki.fi)
  • Two experiments were performed on regular HEK-cells: inhibitor experiment with KYP-2047 (1 or 10 µM) and overexpression experiment (transfection with either active or inactive hPREP plasmid). (helsinki.fi)
  • In vitro experiments withcardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibilityof BC-Ppy composites. (ibecbarcelona.eu)
  • Unexpected results came out of rescue experiments, which were done to assess how much is p53 responsible for the defects observed in stem cells after losing centrosome. (databasefootball.com)
  • Single cell experiments [ 11 ] could provide iterative validation of models with experimental results. (lu.se)
  • To systematically study such transitions, we introduce a method that simultaneously measures protein expression and thermal stability changes in cells and provide the web-based visualization tool ProteoTracker. (nature.com)
  • Fig. 1: Protein thermal stability and expression changes in cell-type transitions. (nature.com)
  • However, subsequent in depth analyses dismissed this possibility, showing that stem cells do pay a price for losing their centrosome. (databasefootball.com)