Pluripotent hematopoietic stem cells. Medical search. Frequent questions

Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. (nature.com)
Our limited understanding of the complex cellular composition, anatomy, and function of this tissue has slowed the establishment of effective tissue regenerative (repair) and/or cell replacement (transplantation) therapies. (ohsu.edu)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
Stem cell transplantation is performed after high-dose chemotherapy (HDC) to restore a cancer patient's blood and immune cell production capacity. (bdbiosciences.com)
30,000 patients with blood-related malignancies receive HDC, which, if the response is satisfactory, could subsequently be followed by hematopoietic stem cell transplantation (HSCT). (bdbiosciences.com)
1 Improvements in transplantation techniques, including the wider use of cell selection, have contributed to a significant reduction in the morbidity and mortality associated with conventional transplantation. (bdbiosciences.com)
3 Autologous stem cell transplantation almost exclusively uses peripheral blood, while for allogeneic SCT, T-lymphocytes in the peripheral blood could pose some danger. (bdbiosciences.com)
The number of viable CD45+/CD34+ cells will determine the quality of the harvested specimen for transplantation. (bdbiosciences.com)
An accurate measurement of CD34 is critical for dose requirement protocols in stem cell transplantation. (bdbiosciences.com)
The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
This book includes discussions of management and treatment strategies for hemoglobin disorders, such as transfusions, iron-chelating agents, gene therapy, and stem cell transplantation. (cshlpress.com)
Treatment is often supportive, but Janus kinase 2 ( JAK2 ) inhibitors, such as ruxolitinib , fedratinib , pacritnib, or momelotinib may decrease symptoms, and stem cell transplantation may be curative. (msdmanuals.com)
The first type of tissue engineering using stem cells was hematopoietic stem cell transplantation (HSCT), a surgical procedure in which hematopoietic stem cells (HSCs) are infused into a host to treat a variety of blood diseases, cancers, and immunodeficiencies. (asu.edu)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases (such as Krabbe disease), including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses as well as peroxisome disorders such as X-linked adrenoleukodystrophy. (medscape.com)
Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)
Some evidence indicates that at least in certain disorders, combination ERT and hematopoietic stem cell transplantation together might be superior to hematopoietic stem cell transplantation alone in patients who are appropriate candidates. (medscape.com)
The availability of both ERT and hematopoietic stem cell transplantation has prompted ongoing consideration of newborn screening efforts to diagnose lysosomal storage diseases. (medscape.com)
The idea is that these will be analogous to the O-negative "universal-donor" blood type and could be employed for all cell-based transplantation therapies in patients with immune rejection, said Dr Cowan. (medscape.com)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases, including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses. (medscape.com)

Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
All GS-2 iPSC clones displayed a normal karyotype (46XX or 46XY) and were shown to express the same RAB27A gene mutation that was present in the original somatic donor cells. (biomedcentral.com)
Especially in cases where sufficient cells cannot be obtained due to the rarity of the disorder, iPSC models have shown great potential in providing research material to develop new treatments or diagnostics, but also to study the pathophysiology of the diseases [ 10 , 12 ]. (biomedcentral.com)
Studies employ a transgenic animal model of spondyloarthritis and cells from human subjects including induced pluripotent stem cells (iPSC), to evaluate effects of HLA-B27 expression and misfolding on ER stress and its consequences for cytokine production and cellular function. (nih.gov)
In this study, we compared hematopoietic differentiation capacity of 35 human iPSC lines derived from four different tissues and four embryonic stem cell lines. (nih.gov)
The analysis revealed that hematopoietic commitment capacity (PSCs to hematopoietic precursors) is correlated with the expression level of the IGF2 gene independent of the iPSC origins. (nih.gov)
There are two types of stem cell manufacturing: embryonic stem cell production, derived from early-stage embryos, and induced pluripotent stem cell (iPSC) production, which reprograms adult cells to an embryonic-like state. (imarcgroup.com)
Though the current process for toxicity testing largely evolves around the use of in vivo animal work, more and more studies rely on human induced pluripotent stem cell (iPSC)-derived hepatocytes as a viable and up-and-coming alternative of determining toxicity. (enzolifesciences.com)

While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
mesenchymal stem cells are most commonly used because they are easy to obtain and present no ethical problems. (springer.com)
Our investigation indicates that most of these studies examined endothelial progenitor cells, hematopoietic stem cells, mesenchymal stem cells, adipose-derived stem cells, and a few others were about less-discussed types of stem cells such as cardiac stem cells, myeloblasts, and induced pluripotent stem cells. (magiran.com)

Our goal is to generate a toolbox of human embryonic stem cell lines to monitor blood forming (hematopoietic) stem cell development in culture. (ca.gov)
To help overcome these bottlenecks, we will generate a suite of human embryonic stem cell reporter lines that can be used to monitor key milestones in blood stem cell development. (ca.gov)
Before that, Dr. Weiss was the Associate Director for The Human Embryonic Stem Cell Core Facility at The Children's Hospital of Philadelphia. (uthsc.edu)
Bisulfite converted genomic DNA lysates from induced pluripotent stem cell and embryonic stem cell lines were hybridized to Illumina HumanMethylation450 BeadChip. (nih.gov)
We explore the method on three circuits for haematopoiesis and embryonic stem cell development for commitment and reprogramming scenarios and illustrate how the method can be used to determine sequential steps for onsets of external factors, essential for efficient reprogramming. (lu.se)

In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
Differentiation of induced pluripotent stem cells (iPSCs) into stromal cells and hematopoietic precursors. (nih.gov)
The variation among induced pluripotent stem cells (iPSCs) in their differentiation capacity to specific lineages is frequently attributed to somatic memory. (nih.gov)
These data demonstrated that variations in the hematopoietic differentiation capacity of iPSCs are not attributable to somatic memories of their origins. (nih.gov)
These new types of cells are called induced pluripotent stem cells (iPSCs). (healthline.com)
To create iPSCs, scientists genetically reprogram the adult stem cells so they behave like embryonic stem cells. (healthline.com)
Induced Pluripotent Stem Cells (iPSCs) are cells derived from non-pluripotent cells, such as adult somatic cells, that are genetically manipulated so as to return to an undifferentiated, pluripotent state. (asu.edu)
Research on iPSCs, initiated by Shinya Yamanaka in 2006 and extended by James Thompson in 2007, has so far revealed the same properties as embryonic stem cells (ESCs), making their discovery potentially very beneficial for scientists and ethicists alike. (asu.edu)
The recent development of induced pluripotent stem cells (iPSCs) and related technologies has caught the attention of scientists, activists, politicians, and ethicists alike. (asu.edu)
IPSCs gained immediate international attention for their apparent similarity to embryonic stem cells after their successful creation in 2006 by Shinya Yamanaka and in 2007 by James Thompson and others. (asu.edu)

The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)

Cell Stem Cell 19 , 569-572 (2016). (nature.com)
Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)

These lines will serve as tools to identify factor combinations to improve the in vitro differentiation of hESCs to functional blood stem cells. (ca.gov)
The pluripotent stem cell technology represents a major breakthrough for studying disease because these cells express human genes of interest, they can be differentiated in vitro to the three germ layers and derivative tissues, they can be genetically manipulated, and they allow for easy access to cells for in-depth structural, functional, and molecular characterization. (chop.edu)
Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
Induced pluripotent stem cells are most useful when combined with a high throughput in vitro assay that can recognize hepatotoxicity. (enzolifesciences.com)
Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
Stem cell senescence in the in vitro expansion stage is an essential concern in clinical procedures of cell therapy. (magiran.com)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)

These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
Overview of Myeloproliferative Neoplasms Myeloproliferative neoplasms are clonal proliferations of bone marrow hematopoietic stem cells, which can manifest as an increased number of functionally normal platelets, red blood cells (RBCs). (msdmanuals.com)

Hematopoietic specification from human pluripotent stem cells: current advances and challenges toward de novo generation of hematopoietic stem cells. (bvsalud.org)

In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. (bdbiosciences.com)
Depletion of Runx1 with antisense morpholino oligonucleotides abrogated the development of both blood and vessels, as demonstrated by loss of circulation, incomplete development of vasculature and the accumulation of immature hematopoietic precursors. (biologists.com)

In addition to using renewable sources of pluripotent stem cell-derived human hematopoietic lineages for therapeutic and investigative applications, she is genome editing pluripotent stem cells using the CRISPR-CAS9 technology. (chop.edu)
Join us for the next Stem Cell Stars Seminar featuring Andrea Ditadi, Research Group Leader of the Human hematopoietic development and disease modeling unit at IRCCS Ospedale San Raffaele in Italy! (lu.se)

Our studies also suggest that these hESC reporter lines will become valuable for investigators working on optimizing stem cell differentiation and culture for other tissues, as many of these reporter genes are not only restricted to HSC. (ca.gov)
Stem cell therapies hold immense promise in addressing these ailments by regenerating damaged tissues and organs. (imarcgroup.com)
These stem cells come from developed organs and tissues in the body. (healthline.com)
The ability to generate isogenic lines has eliminated the clonal heterogeneity observed when comparing cell lines of different genetic backgrounds during differentiations to derivative tissues and downstream functional applications. (chop.edu)
The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
In the future they may be used to replace cells and tissues that have been damaged or lost due to disease. (yourgenome.org)
find ways of using stem cells to replace cells or tissues that have been damaged or lost. (yourgenome.org)
If we understand stem cell development, we may be able to replicate this process to create new cells, tissues and organs . (yourgenome.org)
Cells, tissues and organs can sometimes be permanently damaged or lost by disease, injury and genetic conditions . (yourgenome.org)
Hemoglobin is an iron-containing protein in red blood cells that transports oxygen to tissues throughout the body. (cshlpress.com)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)

Wutz, A. Haploid mouse embryonic stem cells: rapid genetic screening and germline transmission. (nature.com)
Genetic modification and screening in rat using haploid embryonic stem cells. (nature.com)
Additionally, innovations in genetic editing techniques, like CRISPR-Cas9, offer precise control over stem cell characteristics, further expanding their therapeutic potential. (imarcgroup.com)
These cells have been successfully used to treat children with blood cancers, such as leukemia, and certain genetic blood disorders. (healthline.com)
She is continuing this work using human pluripotent stem cells to study pediatric and adult genetic diseases, including hematological disorders. (chop.edu)
hemoglobinopathies (e.g., sickle cell disease) and thalassemias are some of the most common human genetic diseases worldwide. (cshlpress.com)
It is an indispensable reference for biochemists, geneticists, cell and developmental biologists, physiologists, and all who are interested in reducing the medical burden of these common genetic diseases. (cshlpress.com)
Olariu V, Manesso E, commitment and Peterson C. 2017 A deterministic method for estimating free energy genetic network reprogramming paths landscapes with applications to cell commitment and reprogramming paths. (lu.se)

Highly Efficient CRISPR-Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells. (chop.edu)

Leeb, M. & Wutz, A. Derivation of haploid embryonic stem cells from mouse embryos. (nature.com)
Embryonic stem cells come from human embryos that are three to five days old. (healthline.com)
Scientists have applied somatic cell nuclear transfer to clone human and mammalian embryos as a means to produce stem cells for laboratory and medical use. (asu.edu)
Transient expression of runx1 in cloche embryos resulted in partial rescue of the hematopoietic defect. (biologists.com)

Areas of research focus are beta cell replacement therapies for the treatment of diabetes, the identification of lung progenitor cell populations involved in lung repair, and in the diagnosis of pancreatic cancer. (ohsu.edu)
The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)
2 Evaluation of harvest adequacy requires the use of reliable progenitor cell assays and this is usually achieved by CD34 cell counting using flow cytometry. (bdbiosciences.com)
Flow cytometric applications for CD34+ cell identification and enumeration provide a rapid, quantitative and reproducible method to evaluate the progenitor cell population. (bdbiosciences.com)
We searched the databases such as EMBASE, PubMed, and Web of Science with the keywords "stem cell," "progenitor cell," "satellite," "senescence" and excluded the keywords "cancer," "tumor," "malignancy" and "carcinoma" until June 2020. (magiran.com)

An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. (nature.com)
CML offers more-than-minimally manipulated cell and tissue procedures performed aseptically in our cleanroom in accordance with GMP / GTP quality compliance requirements . (cincinnatichildrens.org)
Our laboratory, in collaboration with the laboratory of Jeffrey Whitsett, M.D., at the Cincinnati Children's Hospital, is generating and characterizing a panel of monoclonal antibodies targeting cell-surface markers on different subsets of pulmonary cells involved in the process of pulmonary tissue regeneration. (ohsu.edu)
This means they can potentially produce new cells for any organ or tissue. (healthline.com)
Induced pluripotent stem cell-derived hepatocytes have a primary tissue-like phenotype, constant and indefinite disposal, and the potential to establish genotype-specific cells from different individuals, which make them an interesting means of predicting drug-induced toxicity. (enzolifesciences.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
We can grow tissue and organ structures from stem cells, which can then be studied to find out how they function and how they are affected by different drugs . (yourgenome.org)
A feedback loop involving erythropoietin helps regulate the process of erythropoiesis so that, in non-disease states, the production of red blood cells is equal to the destruction of red blood cells and the red blood cell number is sufficient to sustain adequate tissue oxygen levels but not so high as to cause sludging, thrombosis, or stroke. (wikidoc.org)
The uniqueness of tissue engineering is that it can restore normal structure in addition to repairing tissue function, and is often accomplished using stem cells. (asu.edu)
Mechanisms of bronchocontriction mediated by mast cell nerve interaction in human atopic lung tissue. (fraunhofer.de)
Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)

MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)

Lack of expression of lineage markers is used in combination with detection of several positive cell-surface markers to isolate HSCs. (wikipedia.org)
Divergent functions of hematopoietic transcription factors in lineage priming and differentiation during erythro-megakaryopoiesis. (uthsc.edu)
More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
They establish a link between clonal expansion and lineage plasticity of T cells and reveal the involvement of granzyme K-expressing CD8 T cells in TPE pathogenesis. (rupress.org)
or movements in a free energy landscape such that lineage choices are paths between stable cell states. (lu.se)

Inducible Gata1 suppression expands megakaryocyte-erythroid progenitors from embryonic stem cells. (uthsc.edu)

Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. (justia.com)
In particular, embodiments of the method involve inhibition of both Rac1 and Rac2 GTPases to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject. (justia.com)
Three pulmonary veno-occlusive disease patients who carried the compound heterozygous variants of EIF2AK4 and two healthy controls were recruited and induced pluripotent stem cells were generated from human peripheral blood mononuclear cells. (stemcellsciencenews.com)
After these stages, the cell is released from the bone marrow, and ultimately becomes an "erythrocyte" or mature red blood cell circulating in the peripheral blood. (wikidoc.org)

CD34, a transmembrane phosphoglycoprotein, is present on immature hematopoietic precursor cells and all hematopoietic colony-forming cells in bone marrow and blood, including unipotent and pluripotent progenitor cells. (bdbiosciences.com)
4 CD34 expression is historically related to hematopoietic cells and it is considered as the marker of HSCs. (bdbiosciences.com)
6 Fluorochrome-conjugated monoclonal antibodies directed against CD34 molecule can be used to identify CD34+ cells by flow cytometry. (bdbiosciences.com)
The BD ® Stem Cell Enumeration (SCE) Kit provides simultaneous enumeration of viable dual-positive CD45+/CD34+ hematopoietic stem cell populations in CD34+ absolute counts (cells/µL) as well as the percentage of the total viable leucocyte count that is CD34+ (%CD34). (bdbiosciences.com)
Single-platform flow cytometric absolute cell counting protocols have been shown to provide increased robustness of CD34 enumeration by limiting potential sources of imprecision. (bdbiosciences.com)
Of them, 287 and 274 patients collected ≥2 and ≥4×106 CD34 + cells/kg, respectively, with a median of 9.9×106 CD34 + cells/kg collected. (stemcellsciencenews.com)

His research interests are to better understand the mechanisms of normal red blood cell development and use this information to treat common blood disorders, including sickle cell disease and betathalassemia. (uthsc.edu)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)

This change in gene activity blocks the maturation (differentiation) of blood cells and leads to the production of abnormal, immature white blood cells called myeloid blasts. (medlineplus.gov)
In the process of red blood cell maturation, a cell undergoes a series of differentiations . (wikidoc.org)
In the process of maturation a basophilic pronormoblast is converted from a cell with a large nucleus and a volume of 900 µm 3 to an enucleated disc with a volume of 95 µm 3 . (wikidoc.org)

Overview of Leukemia Leukemia is a malignant condition involving the excess production of immature or abnormal leukocytes, which eventually suppresses the production of normal blood cells and results in symptoms. (msdmanuals.com)

Although hematopoietic stem cells (HSC) are the best characterized and the most clinically used adult stem cells, efforts are still needed to understand how to best ex vivo expand these cells. (stemcell.com)
Adult stem cells have a misleading name, because they are also found in infants and children. (healthline.com)
For example, hematopoietic stem cells are a type of adult stem cell found in bone marrow. (healthline.com)
Adult stem cells can't differentiate into as many other types of cells as embryonic stem cells can. (healthline.com)
Scientists have recently discovered how to turn adult stem cells into pluripotent stem cells. (healthline.com)
Adult stem cells don't present any ethical problems. (healthline.com)
The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
Adult stem cells supply new cells as an organism grows and to replace cells that get damaged. (yourgenome.org)
Induced' means that they are made in the lab by taking normal adult cells, like skin or blood cells, and reprogramming them to become stem cells. (yourgenome.org)
Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)

Patient-derived induced pluripotent stem cells recapitulate hematopoietic abnormalities of juvenile myelomonocytic leukemia. (uthsc.edu)
Dr. French and her colleagues have shown a number of hematopoietic pathologies to be successfully recapitulated through differentiation of patient-derived induced pluripotent stem cells, including Glanzmann thrombasthenia, Down syndrome, and juvenile myelomonocytic leukemia. (chop.edu)

Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
Scientists are using induced pluripotent stem cells to produce new RPE cells in the lab that can then be put into a patient's eye to replace the damaged cells. (yourgenome.org)
Seema Bachoo Immunotherapy is a type of cancer treatment that makes use of a patient's own immune system to target and destroy cancer cells, with the aim of eliminating cancer from the body. (personalizemymedicine.com)

The global stem cell manufacturing market is influenced by increasing investments in stem cell research and regenerative medicine by governments and private organizations. (imarcgroup.com)
The global stem cell manufacturing market is experiencing a significant upsurge due to the substantial investments pouring into stem cell research and regenerative medicine. (imarcgroup.com)
Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
Somatic cell nuclear transfer (SCNT) is a technology applied in cloning, stem cell research and regenerative medicine. (asu.edu)
If successful, this proposed work could have an enormous impact on regenerative medicine, leading the way to rigorously tested universal-donor stem cells that could be grown and differentiated into very large numbers of cells, made widely available to all medical institutions and used on demand to treat patients with type 1 diabetes and a variety of degenerative diseases, he concluded. (medscape.com)

The process of generating thrombocytes (BLOOD PLATELETS) from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via the MEGAKARYOCYTES. (bvsalud.org)

Our in vivo tracking tools will be broadly relevant and not restricted to studying the in vivo biology of blood forming cells. (ca.gov)
research - to help us understand the basic biology of how living things work and what happens in different types of cell during disease. (yourgenome.org)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
You can put islet cells into someone with type 1 diabetes and they can leave the hospital 3 days later with no diabetes….But you're not going to get very far with human donors," press briefing moderator Gordon C Weir, MD, professor of medicine at Harvard Medical School, and cohead of the Joslin Diabetes Center's section on islet cell and regenerative biology, told Medscape Medical News . (medscape.com)

Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)
A scientist here at the Wellcome Genome Campus working on induced pluripotant stem cells. (yourgenome.org)

The Cell Manipulations Laboratory's experienced scientists can help you develop and scale up your ex-vivo gene transfer and cell therapy clinical trials or simply transfer your existing at-scale technology to our clean facilities which will enable you to move your research to a safe and effective patient treatment plan. (cincinnatichildrens.org)
Researchers and clinicians can contact us for more information about how we can help you with your cell therapy and gene transfer needs. (cincinnatichildrens.org)
However, the supply of donor-derived islets will never meet the needs of the large number of patients who could potentially benefit from this cell-based therapy. (ohsu.edu)
Stem cell therapy in India is a safe, affordable, and legal treatment that relieves your ailments without any medicine. (medicaltourismco.com)
Dr. Anand Srivastava: What is Stem Cell Therapy? (medicaltourismco.com)
The Indian stem cell therapy ranges between $1,500 to $30,000 in India*, which further depends on the condition being treated. (medicaltourismco.com)
Therefore, the cost of stem cell therapy in India lets you save nearly 50% of the price back in the USA. (medicaltourismco.com)
All these diseases are readily treated by Stem Cell Therapy in India at a reasonable cost. (medicaltourismco.com)
Medical Tourism Corporation has partnered with the Global Institute Of Stem Cell Therapy and Research (GIOSTAR). (medicaltourismco.com)
While this paper is more focused on mechanisms, the area of wound healing is one of the most promising in the cell therapy space. (ipscell.com)
therapy - to replace lost or damaged cells that our bodies can't replace naturally. (yourgenome.org)
Cell Replacement Therapy for Parkinson's Disease - Evaluating the potential of autologous grafting. (lu.se)
What is CAR-T cell therapy? (personalizemymedicine.com)
In addition to the aging phenomenon, stem cell senescence has been investigated in various concepts such as cancer, adverse drug effects, and as a limiting factor in cell therapy. (magiran.com)

Currently, we lack markers that we can use to pinpoint the developmental stage of human ES cell derived candidate hematopoietic stem cells (HSC), and whether they have established the correct molecular machinery required for proper function. (ca.gov)

The primary myelofibrosis progeny cells stimulate bone marrow fibroblasts (which are not part of the neoplastic transformation) to produce excessive collagen. (msdmanuals.com)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)

While t(8;21) is important for leukemia development, a mutation in one or more additional genes is typically needed for the myeloid blasts to develop into cancerous leukemia cells. (medlineplus.gov)
Transcription factor RUNX1 promotes survival of acute myeloid leukemia cells. (medlineplus.gov)
Immunophenotypic and Molecular Features of Acute Myeloid Leukemia with Plasmacytoid Dendritic Cell Differentiation Are Distinct from Blastic Plasmacytoid Dendritic Cell Neoplasm. (atlasgeneticsoncology.org)
Several possible mechanisms were considered for the induction of human leukemia, such as clastogenic damage to circulatory stem cells. (who.int)

The SLAM (Signaling lymphocyte activation molecule) family is a group of more than 10 molecules whose genes are located mostly tandemly in a single locus on chromosome 1 (mouse), all belonging to a subset of the immunoglobulin gene superfamily, and originally thought to be involved in T-cell stimulation. (wikipedia.org)
hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)
Fig. 2: Analysis of cell-essential genes. (nature.com)
transcription factor (activator) for various hematopoietic-specific genes. (atlasgeneticsoncology.org)

Eventually, the cells begin to differentiate, taking on a certain function in a part of the body. (healthline.com)
They can differentiate into all types of specialized cells in the body. (healthline.com)
The breakthrough has created a way to "de-differentiate" the stem cells. (healthline.com)
This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
Stem cells are undifferentiated cells that can shift, or differentiate, into specialized types of cells and serve as a repair system in the body by dividing indefinitely to replenish other cells. (asu.edu)
Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)

To deliver on that promise, our laboratory is collaborating with the laboratories of Markus Grompe, M.D., OHSU, and Gordon Keller, Ph.D., University Health Network, Toronto, Canada, in the development and characterization of monoclonal antibodies directed against cell surface molecules expressed by pancreatic cell subsets as well as pancreatic progenitor cells derived from pluripotent stem cells. (ohsu.edu)
Characterization of hematopoietic stem and progenitor cells generated by teratoma formation with human induced pluripotent stem cells. (fraunhofer.de)
Non-small cell lung cancer primary tumor model generation, characterization and treatment. (fraunhofer.de)
Genomic characterization of lymph node-derived disseminated prostate cancer cells. (fraunhofer.de)

Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
These factors may regulate myeloid versus lymphoid balance with age, and can potentially mitigate the long-term deleterious effects of inflammation that lead to hematopoietic pathologies. (biorxiv.org)
Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)

Endothelial cell α-globin and its molecular chaperone α-hemoglobin-stabilizing protein regulate arteriolar contractility. (uthsc.edu)
Replace fenestrated endothelial cell swelling. (sadlerland.com)

Oct-4 (POU5F1) is a transcription factor that is critically involved in the self-renewal of pluripotent stem cells, and its expression is commonly used as a marker for pluripotency. (stemcell.com)
runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl , and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche . (biologists.com)

There are several types of stem cells that can be used for different purposes. (healthline.com)

Griscelli syndrome type 2 (GS-2) is a rare, autosomal recessive immune deficiency syndrome caused by a mutation in the RAB27A gene, which results in the absence of a protein involved in vesicle trafficking and consequent loss of function of in particular cytotoxic T and NK cells. (biomedcentral.com)
Immunogenicity is reduced by removing the major histocompatibility complex molecules from the stem cells with gene editing, and tolerance induced via "immune editing. (medscape.com)

Our goal is to develop tools that address major bottlenecks that have prevented the generation of blood forming stem cells in culture for therapeutic use. (ca.gov)
The advantages of stem cell manufacturing are manifold, as it offers a renewable source of cells for research, drug testing, and potential therapeutic applications. (imarcgroup.com)
She is an internationally recognized researcher involved in multi-investigator teams that utilize pluripotent stem cells for modeling human disease to study mechanism, development, and establish new therapeutic modalities. (chop.edu)
Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)

In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
In many cases, successfully mobilization is not effected in the patient and inadequate numbers of hematopoietic stem cells are harvested from these patients using current methods. (justia.com)
Quantitating the CD34+ cell population can also be useful during mobilization as well as for determining the optimal timing of apheresis sessions to make sure that enough CD34+ cells have been harvested. (bdbiosciences.com)
A Prospective, Multicenter Study on Hematopoietic Stem-Cell Mobilization with Cyclophosphamide Plus Granulocyte Colony-Stimulating Factor and 'On-Demand' Plerixafor in Multiple Myeloma Patients Treated with Novel. (stemcellsciencenews.com)

The major shortcoming in generating functional hematopoietic stem cells (HSC) from pluripotent stem cells is the lack of adequate markers for specification and maintenance of functional human HSCs. (ca.gov)
citation needed] Therefore, there are many techniques to isolate haematopoietic stem cells (HSCs). (wikipedia.org)
HSCs can be identified or isolated by the use of flow cytometry where the combination of several different cell surface markers are used to separate the rare HSCs from the surrounding blood cells. (wikipedia.org)
however, recent work has shown that this method excludes a large number of HSCs and includes an equally large number of non-stem cells. (wikipedia.org)
Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
Flow cytometric enumeration of CD34+ HSCs and progenitor cells is an established method for the evaluation of bone marrow and stem cell grafts. (bdbiosciences.com)
UCB, a source of hematopoietic stem cells (HSCs), has garnered attention from scientific and medical communities since its first successful use in a hematopoietic stem cell transplant (HSCT) in 1988. (asu.edu)

These tools will help translate the promise of stem cells to cell based therapies to treat human disease. (ca.gov)
Increasing investments in stem cell research, rising chronic disease prevalence, advancements in biotechnology, collaborations between academia and industry, stringent regulations, and expanding awareness of stem cell therapies are stimulating the market growth. (imarcgroup.com)
Apart from this, the increasing awareness and acceptance of stem cell-based therapies among patients and healthcare professionals is augmenting the market growth. (imarcgroup.com)
This driver is fueled by the promising prospects of stem cell therapies in addressing previously untreatable conditions, such as neurodegenerative diseases and spinal cord injuries. (imarcgroup.com)
and 3) facilitate the purification of cell culture expanded cell populations for cell-based therapies. (ohsu.edu)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)

Zebrafish runx1 is expressed in hematopoietic and neuronal cells during early embryogenesis. (biologists.com)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)

There are many differences between the human and murine hematopoietic cell markers for the commonly accepted type of hematopoietic stem cells. (wikipedia.org)

Cord blood banks are institutions designed to store umbilical cord blood (UCB) stem cells. (asu.edu)

Traditional purification method used to yield a reasonable purity level of mouse hematopoietic stem cells, in general, requires a large (~10-12) battery of markers, most of which were surrogate markers with little functional significance, and thus partial overlap with the stem cell populations and sometimes other closely related cells that are not stem cells. (wikipedia.org)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)

These cells can be used in future studies for the development of novel treatment options and to study the pathophysiology of GS-2 disease. (biomedcentral.com)
In particular, it plays an important role in development of hematopoietic stem cells, early blood cells that have the potential to develop into all types of mature blood cells such as white blood cells, red blood cells, and platelets. (medlineplus.gov)
Research hematology with an emphasis on understanding the mechanisms of normal blood cell development associated diseases. (uthsc.edu)
While working in these companies, Dr. Streeter led research efforts in the engineering of hematopoietic cytokines, the development of anti-tumor vaccines, and the identification of selective inhibitors of autoimmune disease. (ohsu.edu)
Dr. French is involved in a broad spectrum of projects using these cells to model human development and disease. (chop.edu)
His work is focused on T cell receptor signaling and the development of T cell subsets. (rupress.org)
EMBO Journal] The authors provide an in-depth review of the metabolic and epigenetic interplay regulating hematopoietic stem cell fate, and discuss the influence of metabolic stress stimuli, as well as alterations occurring during leukemic development. (stemcellsciencenews.com)
All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
Building on Andrea's previous work defining the origin and the mechanisms regulating the generation of blood cells during human development, the Ditadi lab investigates the regulation of normal and pathological hematopoietic development. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)

Stem cell niche. (bvsalud.org)

True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)

In the early fetus, erythropoiesis takes place in the mesodermal cells of the yolk sac . (wikidoc.org)

Anatomy46

Organisms13

Diseases15

Chemicals and Drugs38

Analytical, Diagnostic and Therapeutic Techniques and Equipment40

Psychiatry and Psychology1

Phenomena and Processes38

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science1

Named Groups2

Health Care2

Transplantation19

IPSC8

Mesenchymal stem4

Embryonic Stem5

IPSCs11

Hematopoiesis1

20162

Vitro7

Bone marrow hematopoietic2

Generation of hematopoietic1

Precursors2

Human hematopoietic2

Tissues13

Genetic8

CRISPR-Cas91

Embryos4

Progenitor cell6

Tissue14

Lineages3

Lineage5

Progenitors1

Peripheral blood5

CD346

Disorders2

Maturation3

Immature1

Adult10

Juvenile Myelomon2

Patient's3

Regenerative medicine6

Blood platelets1

Biology4

Genome2

Therapy14

Developmental1

Progeny2

Leukemia4

Genes4

Differentiate6

Characterization4

Myeloid5

Endothelial2

Transcription2

Types of stem cells1

Gene2

Therapeutic5

Mobilization5

HSCs7

Therapies6

Neuronal2

Murine1

Umbilical cord1

Populations4

Differentiation capacity1

Development10

Niche1

Self-renewal1

Yolk sac1